Technique and process for frozen-preserving Chinese sturgeon semen under super-low temperature
An ultra-low temperature freezing, Chinese sturgeon technology, applied in the preservation of human or animal body, household refrigeration devices, lighting and heating equipment, etc., can solve the problem of not being able to meet the needs of sperm preservation, and achieve the effect of stable pH value
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Embodiment 1
[0028] Embodiment 1: Take the freezing 500 milliliters of Chinese sturgeon fresh essence as an example below to illustrate the specific operation process:
[0029] 1. Squeeze the abdomen of the mature male Chinese sturgeon and collect more than 500ml of fresh semen. During the collection process, the sperm should be absolutely avoided from contacting with water. The collected fresh semen should be put into the sealed container after oxygenation according to the amount of 100ml per bag. Put 5 bags in total in plastic bags, and then store them in a refrigerator with a temperature of 4°C;
[0030] 2. Microscopic examination under a microscope of 10×10 times, the computer-aided detection of fresh semen has an average liveness of 85%, the average curve velocity (VCL) of more than 160 microns / second, and the average linear velocity of more than 90 microns / second , it can be frozen when it reaches the standard;
[0031] 3. Preparation of Chinese sturgeon sperm dilution: 2000 ml dilu...
Embodiment 2
[0038] Embodiment 2: Take 1000 milliliters of frozen Chinese sturgeon as an example below, and 4000 milliliters of diluent is needed, and its configuration is as follows:
[0039] 20 ml of 1 mole of Tris-HCl, 30 ml of 1 mole of sucrose, 2 ml of 1 mole of potassium chloride, fixedly dissolved in distilled water to 1000 ml, adjusted to pH 8.5 with HCl, prepared 4 times, a total of 4000 ml was prepared, stored in a refrigerator at 4°C for later use ;
[0040] Other operating methods are the same as in Embodiment 1 above.
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