The invention discloses a method for preparing simulated
cellulase. The method comprises the following steps: taking a
cellulose material, washing, removing the impurities,
drying,
grinding, and screening, thereby obtaining
cellulose powder; uniformly mixing and stirring the obtained
cellulose powder with
ionic liquid according to a
mass ratio of 1-20:100, preserving the temperature, performing assisted
microwave treatment so as to expand the fibers, washing to remove the
ionic liquid, and
drying to remove the
moisture, thereby obtaining a primary sample; adding the obtained primary sample into a
crucible, putting the
crucible into a
tube furnace, and performing heating treatment under
gas protection; adding a mixed acid, adding water and washing to remove the impurities, and
drying to obtain dried
powder, thereby obtaining the sample powder; adding the obtained sample powder into an
organic base, stirring, heating, reacting, and washing by using a cleaning
solvent, thereby obtaining the simulated
cellulase. The simulated
cellulase prepared by the method is high in
binding ability with a substrate (a cellulose material) and good in
thermal stability, high-temperature
catalysis can be performed, the
catalytic effect is good, the cost of the raw materials is low, and the cellulase can be repeatedly utilized.