A kind of preparation method of mixed acid simulated cellulase

A technology of cellulase and mixed acids, applied in chemical instruments and methods, organic compound/hydride/coordination complex catalysts, physical/chemical process catalysts, etc., to achieve good fiber degradation effect, high preparation rate, and simple process Effect

Inactive Publication Date: 2017-05-10
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the results of this experiment do not directly catalyze the hydrolysis of cellulose, so there are few reports on the research on simulated cellulose.

Method used

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  • A kind of preparation method of mixed acid simulated cellulase
  • A kind of preparation method of mixed acid simulated cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put 0.1 g of oxalic acid, 0.1 g of succinic acid and 0.05 g of sulfuric acid into a three-necked round bottom flask, slowly add 100 g of ionic liquid [AMIM]Ac at room temperature, and stir magnetically at 80°C to obtain a colorless liquid. Ether extraction and ethyl acetate were used to extract 3 times to remove impurities; and low-boiling impurities such as water were removed by vacuum distillation to obtain the simulated enzyme.

[0023] Enzymatic hydrolysis of cellulose with simulated enzyme: Add 20g of simulated enzyme into the microwave reaction tube, weigh 0.2g of microcrystalline cellulose and add it into the microwave reaction tube. Put it in a microwave reactor with a microwave of 500W and react at 190°C to degrade the cellulose for 4 minutes and the conversion rate can reach 96.5%.

Embodiment 2

[0025] Put 0.1 g of malonic acid, 0.1 g of succinic acid and 0.05 g of sulfuric acid into a three-neck round bottom flask, slowly add 100 g of ionic liquid [MMIM][DMP] at room temperature, and stir magnetically at 20°C to obtain a light yellow liquid. Ether extraction and ethyl acetate were used to extract 3 times to remove impurities; and low-boiling impurities such as water were removed by vacuum distillation to obtain the simulated enzyme.

[0026] Enzymatic hydrolysis of cellulose with simulated enzyme: Add 20g of simulated enzyme into the microwave reaction tube, weigh 0.2g of microcrystalline cellulose and add it into the microwave reaction tube. Place in a microwave reactor with a microwave of 500W and react at 190°C to degrade the cellulose for 4 minutes and the conversion rate can reach 97.2%.

Embodiment 3

[0028] Put 0.1 g of malonic acid, 0.1 g of succinic acid and 0.01 g of hydrochloric acid into a three-neck round bottom flask, slowly add 100 g of ionic liquid [BmpyM]Cl at room temperature, and stir magnetically at 20°C to obtain a light yellow liquid. Ether extraction and ethyl acetate were used to extract 3 times to remove impurities; and low-boiling impurities such as water were removed by vacuum distillation to obtain the simulated enzyme.

[0029] Enzymatic hydrolysis of cellulose with simulated enzyme: Add 20g of simulated enzyme into the microwave reaction tube, weigh 0.2g of microcrystalline cellulose and add it into the microwave reaction tube. Place in a microwave reactor with a microwave of 500W and react at 190°C to degrade the cellulose for 4 minutes and the conversion rate can reach 98.3%.

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Abstract

The invention discloses a method for preparing a simulated cellulose enzyme from mixed acid. According to the method, the simulated enzyme is synthesized at high selectivity and high yield through one-step synthesis reaction by taking polybasic carboxylic acid, inorganic acid and an ionic liquid as raw materials. The synthesized enzyme can be used for efficiently degrading cellulose to prepare reducing sugar. The simulated enzyme can be recycled for many times. The simulated cellulose enzyme prepared by using the method is good in catalytic effect and is not easily degraded so as to be the simulated cellulose enzyme with an application potential.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of mixed acid imitation cellulase. Background technique [0002] Enzyme (German: Enzym, derived from Greek: εvζυμov, "in the yeast") refers to a polymer substance with biocatalytic function. In the enzyme-catalyzed reaction system, the reactant molecule is called a substrate, and the substrate is converted into another molecule through the catalysis of the enzyme. Almost all cellular processes require the participation of enzymes to improve efficiency. Similar to other non-biological catalysts, enzymes speed up the reaction rate by reducing the activation energy (expressed as Ea or ΔG) of chemical reactions. Most enzymes can increase the rate of the reaction they catalyze by millions of times; in fact, enzymes are Provide another path with lower activation energy requirements, so that more reacting particles can have kinetic energy not less t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/04
Inventor 李强季更生李天程张大钊朱珣之屠洁
Owner JIANGSU UNIV OF SCI & TECH
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