8 results about "Alpha interferon" patented technology

The invention discloses a bovine I-type alpha interferon-ferritin fusion protein, a mutant thereof, a preparation method and an application of the bovine I-type alpha interferon-ferritin fusion protein. The bovine I-type alpha interferon is fused with a ferritin subunit, and interferon molecules are highly repeatedly and orderly displayed on the surface of a ferritin nanocage by utilizing the self-assembly characteristic of ferritin, so that the expression level, the structural stability and the antiviral activity of the interferon are remarkably improved. The fusion protein is further subjected to single-site or multi-site rational design mutation, and a mutant with significantly improved antiviral activity and stability is obtained. According to the invention, a silkworm or insect cell eukaryotic expression system is adopted to express the fusion protein or the mutant thereof, and the expression system is safe to operate, simple and convenient in procedure, low in cost and extremely beneficial to large-scale industrial production; the prepared fusion protein or mutant nanoparticles have application prospects in preparation of drugs or reagents for preventing or treating bovine viral diseases.

The present invention relates to the field of genetic engineering technology, specifically disclosing a raccoon dog interferon-α and β-defensin fusion protein, its encoding gene, and its application. The raccoon dog interferon-α and β-defensin fusion protein has the amino acid sequence shown in SEQ ID NO:1. The present invention also provides a gene encoding the fusion protein, a recombinant expression vector, and a host cell. The fusion protein provided by the present invention possesses the dual biological activities of raccoon dog interferon-α and β-defensin, and has certain antiviral and antibacterial activities.

The application discloses a bovine type I alpha interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type I alpha interferon is fused with a ferritin subunit, the self-assembly characteristics of the ferritin are utilized, the interferon molecules are highly repeated and orderly displayed on the surface of the ferritin nanocage, and the expression level, structural stability and antiviral activity of the interferon are significantly improved. The fusion protein is further subjected to rational design mutation at a unit point or multiple points, and a mutant with significantly improved antiviral activity and stability is obtained. The fusion protein or the mutant thereof is expressed by using a silkworm or insect cell eukaryotic expression system, the expression system is safe in operation, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production, and the prepared fusion protein or mutant nanometer particle has application prospects in the preparation of medicines or reagents for preventing or treating bovine viral diseases.

The present invention belongs to the field of interferon genetic engineering, and discloses recombinant pigeon alpha interferon and its application in the preparation of medicines for resisting pigeon paramyxovirus infection. The present invention constructs recombinant pigeon interferon piINF 5, realizes efficient expression and purification in CHO cell expression system, significantly prolongs its half-life and improves stability. Recombinant pigeon interferon piINF 5 has no toxic effect on DF1 and VERO cells, and can effectively stimulate the transcription level of interferon-stimulated genes, can significantly inhibit the replication of pigeon paramyxovirus, reduce viral copy number and hemagglutination value. In vivo animal attack experiment shows that the recombinant pigeon interferon piINF 5 can well protect pigeons from the attack of pigeon paramyxovirus, and the survival rate reaches 80%, and there is no detoxification in the first five days after the attack, and only 20% of animals have detoxification in the later stage. The present invention provides an efficient and stable new drug selection for the treatment of pigeon paramyxovirus disease, with good commercial application prospects.

The invention belongs to the field of biotechnology and veterinary immune preparations, and relates to a porcine IL-2-IFN-alpha-T alpha 1 interferon fusion protein and a preparation method and application thereof.The amino acid sequence of the interferon fusion protein is shown as SEQ ID NO.1, and the interferon fusion protein is composed of recombinant porcine interleukin 2, flexible connecting peptide, recombinant porcine alpha interferon, flexible connecting peptide and thymosin; the amino acid sequence of the recombinant porcine interleukin 2 is as shown in SEQ ID NO. 2; the amino acid sequence of the recombinant porcine alpha interferon is as shown in SEQ ID NO.3; the amino acid sequence of the thymosin is as shown in SEQ ID NO. 4; the amino acid sequence of the flexible connecting peptide is as shown in SEQ ID NO.5. The porcine IL-2-IFN-alpha-T alpha 1 interferon fusion protein provided by the invention has good biological activity and no cytotoxicity, has high antiviral property and immune regulation function, and can effectively improve the antiviral ability of pigs.

The present application relates to a kind of pig alpha interferon mutant and its preparation method and application, the mutant amino acid sequence is as shown in SEQ ID NO.2.Preparation, with original PIFN-alpha gene as template, according to CHO-K1 cell codon bias optimization gene sequence, again with murine IGk signal peptide replaces traditional CHO signal peptide, while the glutamine of N-terminal 155th is replaced by the alanine with anti-degradation characteristics, by introducing N-terminal regular anti-degradation site to extend protein half-life, finally, the nucleotide sequence encoding the mutant is obtained by directional optimization.It is second, the present application is by constructing the recombinant expression vector pCHO1.0-PIFN-Bip containing double promoter synergistic expression system, and it is transferred into CHO cell and is expressed, successfully realize the efficient expression of pig alpha interferon in CHO cell, and it also has significant effect to be applied to the treatment of pig viral infectious disease.

The invention belongs to the technical field of microbial genetic engineering, and discloses a chicken interferon-alpha polypeptide, a coding gene thereof, a recombinant bacterium thereof and application thereof, the chicken interferon-alpha polypeptide is any one of the following: 1) the amino acid sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.5, and leucine at the 35th site is mutated into threonine; 2) the amino acid sequence of the gene is shown as SEQ ID NO.7, leucine at the 35th site is mutated into threonine, and tyrosine at the 122nd site is mutated into glycine; 3) the amino acid sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.9, leucine at the 35th site is mutated into threonine, glutamine at the 82nd site is mutated into arginine, and tyrosine at the 122nd site is mutated into glycine. The coding gene of the chicken interferon-alpha polypeptide is any one of the following genes: 1) the nucleotide sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.6, SEQ ID NO.8, SEQ ID NO.10, SEQ ID NO.18, SEQ ID NO.20 or SEQ ID NO.22;

The invention belongs to the technical field of protein engineering, and particularly relates to a sheep alpha interferon and sheep interleukin 2 fusion protein and a preparation method thereof. The preparation method comprises the following steps: connecting a ShIFNalpha-IL2 fragment to a pET32a vector to obtain a recombinant plasmid pET32a-ShIFNalpha-IL2; transforming into an E.coli BL21 competent cell, and inducing expression; and centrifuging, collecting the strain, resuspending, carrying out ultrasonic treatment, injecting inclusion body BB, completely draining, injecting the supernatant filtrate, completely draining, injecting inclusion body BB, washing, and eluting the target protein in the column by using inclusion body EB to obtain the ShIFNalpha-IL2 recombinant protein. Through SDS-PAGE electrophoresis detection, separation and purification of the fusion protein of the sheep interferon alpha and the sheep interleukin 2 are realized, and the target protein with relatively high purity is obtained. The fusion protein also has a high expression level, can effectively prolong the retention time of interferon in vivo, and improves the half-life period of interferon.