4 results about "Alpha interferon" patented technology

The application discloses a bovine type I alpha interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type I alpha interferon is fused with a ferritin subunit, the self-assembly characteristics of the ferritin are utilized, the interferon molecules are highly repeated and orderly displayed on the surface of the ferritin nanocage, and the expression level, structural stability and antiviral activity of the interferon are significantly improved. The fusion protein is further subjected to rational design mutation at a unit point or multiple points, and a mutant with significantly improved antiviral activity and stability is obtained. The fusion protein or the mutant thereof is expressed by using a silkworm or insect cell eukaryotic expression system, the expression system is safe in operation, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production, and the prepared fusion protein or mutant nanometer particle has application prospects in the preparation of medicines or reagents for preventing or treating bovine viral diseases.

The invention belongs to the field of biotechnology and veterinary immune preparations, and relates to a porcine IL-2-IFN-alpha-T alpha 1 interferon fusion protein and a preparation method and application thereof.The amino acid sequence of the interferon fusion protein is shown as SEQ ID NO.1, and the interferon fusion protein is composed of recombinant porcine interleukin 2, flexible connecting peptide, recombinant porcine alpha interferon, flexible connecting peptide and thymosin; the amino acid sequence of the recombinant porcine interleukin 2 is as shown in SEQ ID NO. 2; the amino acid sequence of the recombinant porcine alpha interferon is as shown in SEQ ID NO.3; the amino acid sequence of the thymosin is as shown in SEQ ID NO. 4; the amino acid sequence of the flexible connecting peptide is as shown in SEQ ID NO.5. The porcine IL-2-IFN-alpha-T alpha 1 interferon fusion protein provided by the invention has good biological activity and no cytotoxicity, has high antiviral property and immune regulation function, and can effectively improve the antiviral ability of pigs.

The present application relates to a kind of pig alpha interferon mutant and its preparation method and application, the mutant amino acid sequence is as shown in SEQ ID NO.2.Preparation, with original PIFN-alpha gene as template, according to CHO-K1 cell codon bias optimization gene sequence, again with murine IGk signal peptide replaces traditional CHO signal peptide, while the glutamine of N-terminal 155th is replaced by the alanine with anti-degradation characteristics, by introducing N-terminal regular anti-degradation site to extend protein half-life, finally, the nucleotide sequence encoding the mutant is obtained by directional optimization.It is second, the present application is by constructing the recombinant expression vector pCHO1.0-PIFN-Bip containing double promoter synergistic expression system, and it is transferred into CHO cell and is expressed, successfully realize the efficient expression of pig alpha interferon in CHO cell, and it also has significant effect to be applied to the treatment of pig viral infectious disease.

The invention belongs to the technical field of microbial genetic engineering, and discloses a chicken interferon-alpha polypeptide, a coding gene thereof, a recombinant bacterium thereof and application thereof, the chicken interferon-alpha polypeptide is any one of the following: 1) the amino acid sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.5, and leucine at the 35th site is mutated into threonine; 2) the amino acid sequence of the gene is shown as SEQ ID NO.7, leucine at the 35th site is mutated into threonine, and tyrosine at the 122nd site is mutated into glycine; 3) the amino acid sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.9, leucine at the 35th site is mutated into threonine, glutamine at the 82nd site is mutated into arginine, and tyrosine at the 122nd site is mutated into glycine. The coding gene of the chicken interferon-alpha polypeptide is any one of the following genes: 1) the nucleotide sequence of the chicken interferon-alpha polypeptide is shown as SEQ ID NO.6, SEQ ID NO.8, SEQ ID NO.10, SEQ ID NO.18, SEQ ID NO.20 or SEQ ID NO.22;