44results about How to "Good antiviral activity" patented technology

The invention relates to gene segments for coding alpha, beta or gamma interferons of pigs and an application thereof. According to the preference of codons of a Pichia stipitis expression system, high-expression codons are selected to renewedly design and synthesize new mature peptide nucleotide sequences of the alpha, beta and gamma interferon of the pigs, and recombinant yeast expression plasmids of pPICZalphaC-IFN-alpha, pPICZalphaC-IFN-beta and pPICZaplhaC-IFN-gamma are successfully constructed, which not only correctly expresses the alpha, beta and gamma interferons of the pigs in Pichia stipitis, but also enables the alpha, beta and gamma interferons of the pigs to be easier to express in the Pichia stipitis with higher expression level, and lays a foundation for the large-scale production and fermentation of the gene engineering.

The invention belongs to the technical field of biological genetic engineering, and discloses technology for constructing and expressing porcine alpha interferon (PoIFN-alpha) and interleukin 2 (PoIL-2) chimeric gene and quickly renaturing and purifying expression protein. The technology constructs mature peptide genes of PoIFN-alpha and PoIL-2 into PoIFN-alpha-linker-PoIL-2 chimeric gene through a genetic flexible linker (linker) (G4S)3 and clones the chimeric gene into a pGEM-T Easy vector by adopting an overlap extension PCR method, and subclones the chimeric gene into a pQE-30 expression vector for prokaryotic expression. The recombinant fusion protein (rPoIFN-alpha-linker-PoIL-2) can be quickly renatured and purified through urea modification, renaturation by low concentration protein renaturing solution, PBS solution dialysis and other steps. The purified rPoIFN-alpha-linker-PoIL-2 fusion protein has proliferation activity for inhibiting vesicular stomatitis virus (VSV) on a cell, is used for prevention and treatment of porcine virosis as a main ingredient of an antivirus preparation, and has high efficiency, broad spectrum, safety and low price.

The invention relates to a composite porcine alpha-IFN gene and a recombinant vector thereof, and belongs to the technical field of genetic engineering biological products. The newly designed gene sequences of composite porcine alpha-IFN are recombined to pPICZ alpha-A vectors, and the vectors are electronically transformed into yeast competent cells; and the positive identification, and the screening and the induced expression of high copy yeast strains are carried out. A pichia pastoris expression system is used for expressing foreign genes so as to be favorable for the commercial production of porcine IFN. Compared with the natural IFN which is expressed by yeast, the expression level of the protein which is expressed by genome increases by 3.17 times. The fluorescent quantitative PCR result shows that the level of PRVmRNA after PK-15 cells treated by 10-6mg/ml of the composite porcine alpha-IFN are re-infected with PRV is obviously lower than the level of PRVmRNA after the PK-15 cells which are not treated by the composite porcine alpha-IFN are infected with the PRV, and the level of PRVmRNA decreases by 37 times.

The invention discloses a bicyclol-carnosine conjugate, and a preparation method and an application thereof. The structural formula of the compound is shown in the specification. By adoption of the bicyclol-carnosine conjugate, the problem of poor oral bioavailability of bicyclol is solved. The preparation method comprises the following steps of: bonding hydroxyl groups of bicyclol and carboxyl groups of carnosine by ester bonds or bonding protected histidine and beta-alanine to the bicyclol in sequence, and then removing protecting groups to obtain the bicyclol-carnosine conjugate. The bicyclol-carnosine conjugate and pharmeceutically-accepted salts thereof can be used for treating the liver injury and improving the liver function.

The invention discloses a method for sulfonating pectin, which comprises the step of sulfonating pectin or triethylamine pectin salt by using sulfur trioxide-pyridine complex in a dimethylsulfoxide (DMSO) environment so as to obtain sulfonated pectin polysaccharide. The method adopts the following two systems: a trioxide-pyridine complex-DMSO system, and a TBA-trioxide-pyridine complex-DMSO system. The method disclosed by the invention is simple in operation and good in sulfonating effect. The sulfonated pectin polysaccharide prepared by the method is small in degradation in the reaction process, small in side reaction, has good anticoagulation, anti-tumor and anti-virus actives and a wide application range.

The invention provides a suspension culture technology of mammal cells capable of efficiently expressing recombinant cat interferon-[omega]2 (rFeIFN-[omega]2). The suspension culture technology is carried out in a bioreactor and comprises the following steps of: (1) setting the initial culture temperature to be 36.5 DEG C, the dissolved oxygen to be 40% and the pH to be 6.8 +/-0.5, and lowering the temperature to 33 DEG C on the sixth day of culture; and (2) supplementing 5%, 5%, 5%, 5% and 3% serum-free feed-batch culture medium on the third, sixth, eighth, tenth and twelfth days of culturing, maintaining the dissolved oxygen to be 40% and the pH to be 6.8 +/-0.5, and finishing culturing on the sixteenth day. The suspension culture technology has the advantages of a simple culture technological process, and long motility keeping time of cells under a condition of high-density growth, target protein expression can be 2g/L or above, antiviral activity is good, production cost is low, and the suspension culture technology is suitable for large-scale industrial application.

Disclosed herein are a natural antiviral agent having high inhibitory activity against a broad spectrum of viruses without adverse side effects, obtainable using lactic acid bacteria isolated from kimchi, and a composition comprising the natural antiviral agent. The natural antiviral agent is safe to the body and can be applied to compositions, such as fodder, beverages, food, medicine, and cosmetics, imparting antimicrobial activity thereto.

The invention relates to a preferred rice black-streaked dwarf virus RNAi multivalent target gene sequence which sequentially comprises 206 bp segment in a rice black-streaked dwarf virus S8 code region, 205 pb in an S10 code region, 203 pb in an S4 code region, wherein a BamH1 restriction enzyme cutting site and three CCG protective bases are added at the 5'end of the S8; an XhoI restriction enzyme cutting site and three ACA protective bases are added at the 3'end of S4. According to the rice black-streaked dwarf virus RNAi multivalent target gene sequence and application, based on a special design and optimization and specific to common 614 bp of RANi target sequence of three genes, namely RBSDV S8, S10, and S4, the specific rice black-streaked dwarf virus RNAi multivalent target gene sequence is acquired, cloned and structured into an RNAi carrier pBDL03 to obtain an RNA silent carrier pBDL03-S8+S10+S4; then RNA silent carrier pBDL03-S8+S10+S4 is transferred to a rice variety Taigeng 394 through an agrobacterium transformation method. T1 generation positive plants have favorable resistance on RBSDV.

The invention discloses a thiazole heterocyclic ring-containing steroid derivative as well as a preparation method and application thereof. A thiazole unit-containing steroid derivative is as shown ina formula I as shown in the following descriptions. The invention also provides a pharmacologically acceptable salt of the thiazole unit-containing steroid derivative. The preparation method providedby the invention is started from simple and easily obtained raw materials; the thiazole heterocyclic ring-containing steroid derivative is obtained through three steps of reactions. The thiazole heterocyclic ring-containing steroid derivative provided by the invention belongs to a compound with a brand-new structure, has better antiviral activity, and is suitable for the preparation of an antiviral medicine.

The invention belongs to the technical field of drug synthesis and agricultural disease control, and particularly relates to an amide-containing ferulic acid derivative as well as a preparation method and application thereof. The synthesized amide-containing ferulic acid derivative compound can be applied to preparation of plant virus resisting agents and can effectively inhibit diseases such as tomato spotted wilf virus and cucumber mosaic virus disease. The compounds Y1, Y2, Y8, Z1 and Z2 in the amide-containing ferulic acid derivative disclosed by the invention show relatively good inhibitory activity on tomato spotted wilf virus and cucumber mosaic virus. Wherein the compound Y2 shows relatively good passivation activity on tomato spotted wilf virus and cucumber mosaic virus, and is superior to positive control medicaments such as ferulic acid, ningnanmycin and virazole. The compound can be used as a medicine or medicament for preventing and treating diseases such as tomato spotted wilf virus and cucumber mosaic virus. The structure of the material is derived from natural products, and the material is environment-friendly, easy to metabolize and degrade, simple in preparation process, relatively stable in physicochemical property and wide in application prospect.

The invention belongs to the biotechnology field, in particular discloses a preparation method of attenuated salmonella transferring chicken gamma interferon. The preparation method is characterized by comprising the following steps: amplifying a gene sequence of a maturation protein of the chicken gamma interferon (chIFN-gamma); obtaining a recombinant eukaryotic expression plasmid; detecting a chIFN-gamma activity unit expressed by the recombinant eukaryotic plasmid; electrically transferring the recombinant plasmid pVAX1<c>-chIFN-gamma into the attenuated salmonella to obtain a strain. By the adoption of the attenuated salmonella transferring chicken gamma interferon, the problems that the interferon is easy to be degraded by gastric acid after being orally taken by the chicken and the protein purifying step is fussy can be avoided effectively. The chIFN-gamma can be prepared by bacteria fermentation, and is easy to store; the chIFN-gamma has obvious NDV (Newcastle disease virus) protection force for chicken, and can provide guarantee for preventing and treating the viral diseases such as NDV in a scale chicken house.

A process for preparing nano-Ag sol includes such steps as preparing the reverse-phase microemulsion from glucolipide-type surfactant, mixing the microemulsion containing reducer with the microemulsion containing silver nitrate while high-speed stirring, preparing Ag nanoparticles, demulsifying, separating, washing, and distributing them in nonpolar solvent. Its advantages are high stability and high antibacterial effect.

The invention belongs to the technical field of medicines, and particularly relates to a biphenyl diarylpyrimidine derivative containing a chiral hydroxymethylene structure as well as preparation andapplication thereof. The biphenyl diarylpyrimidine derivative with the compound structure containing a chiral hydroxymethylene structure comprises medicinal salt, stereochemical isomers, hydrates andsolvates of the biphenyl diarylpyrimidine derivative, X-ray diffraction single crystals of polycrystals or eutectic crystals and single enantiomers of the biphenyl diarylpyrimidine derivative, and precursors and derivatives of the biphenyl diarylpyrimidine derivative with the same biological function. The invention also comprises a preparation method of the compound and an application of a composition containing one or more compounds in related medicines for treating AIDS and the like. In-vitro cell level anti-HIV-1 activity experiment results show that the small molecules have relatively strong anti-HIV-1 biological activity, can significantly inhibit virus replication in MT-4 cells infected by HIV-1 viruses, have relatively low cytotoxicity, and are expected to become anti-HIV candidatedrugs.

The invention discloses a compound with anti-rotavirus activity in Thalictrum scabrifolium Franch. var. leve Franch, and a preparation method thereof. The compound is a chromone alkaloid compound, and the structural formula is as shown in formula (I). The preparation method of the compound comprises the following steps: preparing an extract by taking a Thalictrum scabrifolium Franch. var. leve Franch whole plant as a raw material; treating the extract by silica gel column chromatography to obtain a first eluate; concentrating the first eluate, and performing high-pressure liquid chromatography separation to obtain a second eluate; and finally, carrying out gel column chromatography on the second eluate to obtain the compound as shown in the formula (I). The new chromone alkaloid compound is extracted from the Thalictrum scabrifolium Franch. var. leve Franch whole plant as het raw material, and the compound has outstanding rotavirus resistance activity through rotavirus resistance experiments. The anti-rotavirus activity of the chromone alkaloid compound is disclosed for the first time, a new compound skeleton type is provided for antiviral drug screening, and the chromone alkaloid compound has important significance for development of new antiviral drugs.