33 results about "Vesicular Stomatitis" patented technology

Based on a highly conserved domain of a foot-and-mouth disease virus 3D protein coding gene, a vesicular stomatitis virus N protein coding gene and a swine vesicular disease virus VP1 protein coding gene, the invention designs a specific primer and a probe and develops a multiple fluorescence RT-PCR detection method used for simultaneously detecting the three animal viruses. The detection method can detect 102 copied plasmids containing target amplification sequences in 1 h. The method has high sensitivity and good specificity, and can achieve rapid high-throughput fluorescence RT-PCR detection for single-virus infection or mixed infection by a plurality of viruses.

The invention relates to a kit for detecting vesicular stomatitis antibody and a preparation method thereof. The kit for detecting vesicular stomatitis antibody comprises a reagent I and a reagent II placed respectively, wherein the reagent I is a vesicular stomatitis virus antihelion peridium enzyme linked immunosorbent assay plate and the reagent II is monoclonal antibody IgG-HRP enzyme conjugates, wherein the monoclonal antibody IgG-HRP enzyme conjugates is idiocrasy monoclonal antibody of mouse-anti vesicular stomatitis virus of horse radish peroxidase mark. The invention also provides a preparation method of the kit. The kit adopts competitive ELISA to detect the vesicular stomatitis in serum, and can be used for rapid diagnosis, detection, quarantine and epidemiological investigation of vesicular stomatitis.

The invention discloses primers which can be used for detecting aftosa (type A, type O, and type Asia 1), swine pox and vesicular stomatitis at the same time, a kit consisting of the primers and a preparation method, and a use method of the kit for a dis-illness diagnosis purpose. The invention provides 10 gene sequences of the primers for detecting type A, type O, and type Asia 1 viruses of aftosa, and the kit further comprises two general primers besides the ten primers. Related experiments show that sensitivity of the use method in the invention is higher than sensitivity of general fluorogenic quantitative PCR technology. Compared with the fluorogenic quantitative PCR technology, the detection time is greatly saved; augmentation of non-specific products is avoided; phenomenon of preferences of primers is effectively avoided; and detection sensitivity is improved.

The present invention relates to an immunogenic or vaccine composition to induce an immune response or protective immune response against vesicular stomatitis virus (VSV) in an animal susceptible to VSV. The composition may include a pharmaceutically or veterinarily acceptable vehicle or excipient, and a vector. The vector may contain at least one heterologous nucleic acid molecule(s), expresses in vivo in the animal VSV antigen(s), immunogen(s) or epitope(s) thereof, e.g., VSV G protein and/or VSV N protein and/or VSV M protein. The heterologous nucleic acid molecule(s) may be adjusted to the vector/mammalian cell system by codon optimization. The composition can contain an adjuvant, such as carbomer. Methods for making and using such a composition, including prime-boost regimes and including as to differential diagnosis, are also contemplated.

The invention relates to a pharmaceutical composition for treating tumors or cancers and application thereof, in particular to a pharmaceutical composition which comprises vesicular stomatitis virusesdelivered through direct injection or systemic administration or intratumoral delivery and is combined with PDL1 antibody intravenous administration to generate a synergistic effect in treatment of metastatic lung cancer. Besides, the oncolytic virus after gene mutation and the PDL1 antibody have the characteristic of recognizing tumor cells, normal cells are not damaged, and the oncolytic virusand the PDL1 antibody are sequentially combined for use to generate a synergistic effect in curative effect.

The invention discloses a primer for detecting vesicular stomatitis viruses, a detection kit composed of the primer and a preparation method of the detection kit. The primer for detecting the vesicular stomatitis viruses has gene sequences, namely SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4. A related experiment shows that a nucleic acid of the VSV can be specifically and rapidly amplified by the primer sequence and a specific band can be detected by nucleic acid electrophoresis; and meanwhile, the primer has the specificity. The primer can be applied to preparation of a GeXP diagnostic kit for rapidly identifying the VSV and applied to epidemiologic studies of vesicular stomatitis.

The present invention relates to vesicular stomatitis virus (VSV) matrix (M) protein mutants. One mutant M protein includes a glycine changed to a glutamic acid at position (21), a leucine changed to alanine at position (111) and a methionine changed to an arginine at position (51). Another M protein mutant includes a glycine changed to a glutamic acid at position (22) and a methionine changed to an arginine at positions (48) and (51). These new rVSVs having the mutant M are significantly attenuated and lost virulence, including neurovirulence, and are capable of inducing an immune responses against an antigen of interest. In addition, a rVSV serotype Indiana having the first described M mutant is capable of efficient replication at 31° C., and of poor replication or incapable of replication at about 37° C. or higher.

The present invention relates to vesicular stomatitis virus (VSV) matrix (M) protein mutants. One mutant M protein includes a glycine changed to a glutamic acid at position (21), a leucine changed to a phenylalanine at position (111) and a methionine changed to an arginine at position (51). Another M protein mutant includes a glycine changed to a glutamic acid at position (22) and a methionine changed to an arginine at positions (48) and (51). Yet another VSV M protein mutant includes a glycine changed to a glutamic acid at position (22), a leucine changed to a phenylalanine at position (110) and a methionine changed to an arginine at positions (48) and (51). The present invention is directed also to recombinant VSVs (rVSV) having these M mutants and to vaccines based on the rVSV having the M mutants of the present invention. These new rVSVs having the mutant M were significantly attenuated and lost virulence, including neurovirulence, and are capable of inducing an immune responses against an antigen of interest. In addition, a rVSV serotype Indiana having the first described M mutant is capable of efficient replication at 31° C., and of poor replication or incapable of replication at about 37° C. or higher.

The invention belongs to the technical field of gene engineering, and more specifically relates to a vesicular stomatitis virus recombinant N protein antigen, and a preparation method thereof. The amino acid sequence of the vesicular stomatitis virus recombinant N protein antigen is represented by SEQ ID No.1; and the nucleotide sequence of a gene used for coding the vesicular stomatitis virus recombinant N protein antigen is represented by SEQ ID No.2. The vesicular stomatitis virus recombinant N protein antigen is used for preparing test strips used for rapid detection of vesicular stomatitis virus antibodies; specificity and sensitivity are high; detection is rapid; operation is simple; detection sample amount is small; detection cost is relatively low; and an excellent detection method is provided for vesicular stomatitis virus antibody detection and vesicular stomatitis epidemiological investigation.

The invention discloses a liquid-phase protein chip kit for simultaneously detecting four types of foreign and new animal diseases. Four types of activated fluorescent magnetic microspheres are respectively coupled with the expressed, purified and verified peste des petits ruminants virus protein (PPRV-N), vesicular stomatitis virus protein (VSV-N), schmallenberg virus protein (SBV-N) and bluetongue virus protein (BTV-VP7). The liquid-phase protein chip kit has the characteristics that the four types of foreign and new animal diseases, such as peste des petits ruminants, vesicular stomatitis,schmallenberg and bluetongue, can be simultaneously detected; the parallel detection effect is realized, and the flux is high; about 400 items of 100 samples can be completed each time; the operationis simple, the time is short, and the detection can be completed within 1h or above; the usage amount of samples is small, and multiple items can be detected by only using 0.1 to 1mu L of serum; the specificity and sensitivity are good.

The invention discloses a gene chip of vesicular stomatitis viruses and/or swine vesicular disease viruses and a reagent kit. By means of the gene chip, the detection reagent kit and a detection method, the vesicular stomatitis viruses and/or the swine vesicular disease viruses can be accurately and effectively detected. Besides, the gene chip and the detection reagent kit are high in specificity and sensitivity, low in time consumption, high in detection speed and good in application prospect.

The invention discloses a detection kit, primer and probe capable of simultaneously detecting and identifying a foot-and-mouth disease and vesicular stomatitis. The primer and the probe are shown as sequence tables SEQ ID NO:1 to SEQID NO:14. The invention further discloses a one-step-method multiplex fluorescence RT-PCR detection kit rapid, accurate and convenient to use and simultaneously detecting and identifying the foot-and-mouth disease and the vesicular stomatitis. By adopting the detection method, one-time sampling and one-time analysis can be achieved, the purpose of simultaneously detecting and distinguishing three types of important viruses can be achieved, the working amount and cost for detection is reduced, epidemic disease detection can be completed within the shortest time, and time is won for disease prevention and treatment.

The invention discloses a test kit for epidemic prevention of black cattle and a preparation method thereof. The test kit comprises a detection box body, detection tubes, pressing pistons and samplingneedles, wherein the detection box body is cylindrical; a rotating rod is arranged in and rotationally connected with the detection box body; a knob is fixed to the upper end of the rotating rod; fixed rods are fixed to the side faces of the rotating rod; fixed sleeves are fixed to the ends, away from the rotating rod, of the fixed rods; three fixed rods are in the circumferential direction of the rotating rod; the detection tubes are placed in the two fixed sleeves; each detection tubes is internally connected with the corresponding pressing piston in a sliding mode; and the outer wall of the detection box body is transparent. The detection kit disclosed by the invention can be used for simultaneously detecting various diseases, including main diseases of black cattle, such as foot-and-mouth disease viruses, vesicular stomatitis viruses, bovine lumpy skin disease viruses and the like; the detection efficiency of the kit is high; and meanwhile, the whole structure of the kit is convenient to disassemble, the kit is convenient to clean after detection, pathogen residues can be prevented, and a detection structure is accurate.

The present invention relates to a mutant polypeptide comprising the amino acid sequence of the ectodomain of glycoprotein G of a vesicular stomatitis vims (VSV) strain, wherein said ectodomain comprises the amino acid sequence as set forth in SEQ ID NO: 2 or a sequence having at least 50% of identity with the amino acid sequence as set forth in SEQ ID NO: 2, with at least one substitution of an amino acid residue selected from the group consisting of: a) any amino acid residue located from position 421 to position 429 and any amino acid residue located from position 17 to position 25 of SEQ ID NO:2; or b) any amino acid residue located from a position equivalent to position 421 to a position equivalent to position 429 of SEQ ID NO: 2 and any amino acid residue located from a position equivalent to position 17 to a position equivalent to position 25 of SEQ ID NO: 2, after optimal global alignment with SEQ ID NO:2.

The invention relates to a carboxamide compound and application thereof, belonging to the technical field of preparation of drugs used for preventing and treating animal viral epidemic diseases. The carboxamide compound disclosed by the invention is 2-imino-2H-chromene-3-carboxamide, and the structural formula of the carboxamide compound is shown in the specification. The carboxamide compound disclosed by the invention has no obvious biotoxicity, can effectively control the proliferation of vesicular stomatitis viruses in BHK21 cells at a low dose, delays the occurrence of cytopathic effect, is capable of completely blocking the proliferation of viruses at a large dose, wins over a time window for the prevention and control of epidemic diseases caused by the vesicular stomatitis viruses, and provides a new drug for the treatment of affected individuals.