33 results about "Vesicular Stomatitis" patented technology

The subject invention relates to viruses that are able to replicate and thereby kill neoplastic cells with a deficiency in the IFN-mediated antiviral response, and their use in treating neoplastic disease including cancer and large tumors. RNA and DNA viruses are useful in this regard. The invention also relates to methods for the selection, design, purification and use of such viruses for cancer therapy.

The present invention relates to an immunogenic or vaccine composition to induce an immune response or protective immune response against vesicular stomatitis virus (VSV) in an animal susceptible to VSV. The composition may include a pharmaceutically or veterinarily acceptable vehicle or excipient, and a vector. The vector may contain at least one heterologous nucleic acid molecule(s), expresses in vivo in the animal VSV antigen(s), immunogen(s) or epitope(s) thereof, e.g., VSV G protein and / or VSV N protein and / or VSV M protein. The heterologous nucleic acid molecule(s) may be adjusted to the vector / mammalian cell system by codon optimization. The composition can contain an adjuvant, such as carbomer. Methods for making and using such a composition, including prime-boost regimes and including as to differential diagnosis, are also contemplated.

The invention belongs to the technical field of gene engineering, and more specifically relates to a vesicular stomatitis virus recombinant N protein antigen, and a preparation method thereof. The amino acid sequence of the vesicular stomatitis virus recombinant N protein antigen is represented by SEQ ID No.1; and the nucleotide sequence of a gene used for coding the vesicular stomatitis virus recombinant N protein antigen is represented by SEQ ID No.2. The vesicular stomatitis virus recombinant N protein antigen is used for preparing test strips used for rapid detection of vesicular stomatitis virus antibodies; specificity and sensitivity are high; detection is rapid; operation is simple; detection sample amount is small; detection cost is relatively low; and an excellent detection method is provided for vesicular stomatitis virus antibody detection and vesicular stomatitis epidemiological investigation.

The invention discloses a gene chip for detecting six viruses of zoonosis-borne diseases and the application thereof. The gene chip for the viruses of the zoonosis-borne diseases is a gene chip for detecting at least one virus in six viruses of hepatitis E virus, Nipah virus, rabies virus, Rift Valley fever virus, foot and mouth disease virus and Vesicular stomatitis virus, and is fixed with a hepatitis E virus detection probe, a Nipah virus detection probe, a rabies virus detection probe, a Rift Valley fever virus detection probe, a foot and mouth disease virus detection probe and a Vesicular stomatitis virusdetection probe. The invention also discloses a method for detecting the Rift Valley fever virus, the Nipah virus, the foot and mouth disease virus, the Vesicular stomatitis virus, the hepatitis E virus and / or the rabies virus. The invention can stably and specifically implement generic detection of the six viruses of the zoonosis-borne diseases, has the advantages of simpleness, convenience, quickness, good specificity and high sensitivity, and can be used for detecting clinical samples.

The invention discloses a detection kit, primer and probe capable of simultaneously detecting and identifying a foot-and-mouth disease and vesicular stomatitis. The primer and the probe are shown as sequence tables SEQ ID NO:1 to SEQID NO:14. The invention further discloses a one-step-method multiplex fluorescence RT-PCR detection kit rapid, accurate and convenient to use and simultaneously detecting and identifying the foot-and-mouth disease and the vesicular stomatitis. By adopting the detection method, one-time sampling and one-time analysis can be achieved, the purpose of simultaneously detecting and distinguishing three types of important viruses can be achieved, the working amount and cost for detection is reduced, epidemic disease detection can be completed within the shortest time, and time is won for disease prevention and treatment.

The invention discloses a primer for detecting vesicular stomatitis viruses, a detection kit composed of the primer and a preparation method of the detection kit. The primer for detecting the vesicular stomatitis viruses has gene sequences, namely SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4. A related experiment shows that a nucleic acid of the VSV can be specifically and rapidly amplified by the primer sequence and a specific band can be detected by nucleic acid electrophoresis; and meanwhile, the primer has the specificity. The primer can be applied to preparation of a GeXP diagnostic kit for rapidly identifying the VSV and applied to epidemiologic studies of vesicular stomatitis.

The invention discloses primers which can be used for detecting aftosa (type A, type O, and type Asia 1), swine pox and vesicular stomatitis at the same time, a kit consisting of the primers and a preparation method, and a use method of the kit for a dis-illness diagnosis purpose. The invention provides 10 gene sequences of the primers for detecting type A, type O, and type Asia 1 viruses of aftosa, and the kit further comprises two general primers besides the ten primers. Related experiments show that sensitivity of the use method in the invention is higher than sensitivity of general fluorogenic quantitative PCR technology. Compared with the fluorogenic quantitative PCR technology, the detection time is greatly saved; augmentation of non-specific products is avoided; phenomenon of preferences of primers is effectively avoided; and detection sensitivity is improved.

The world is plagued with several viruses some of which have prevention and treatment tools available, while every so often a new strain will show up without sensitivity to existing drugs. The present invention provides plant-based flavonoid pharmaceutical compositions for inhibition of kinases, particularly phosphatidylinositol-4-kinases (PI4Kiiiβ), AAK1, BIKE, GAK and other transcription factors required for viral entry, replication and survival, and consequent for prevention and treatment of RNA viruses including but not limited to adenoviruses, alphaviruses, coronaviruses, enteroviruses, flaviviruses, hepatitis, herpes, influenza viruses, measles, picornaviruses, vesicular stomatitis and associated disorders. A method for synthesizing the flavonoids and formulation into therapeutic products are also disclosed.

Based on a highly conserved domain of a foot-and-mouth disease virus 3D protein coding gene, a vesicular stomatitis virus N protein coding gene and a swine vesicular disease virus VP1 protein coding gene, the invention designs a specific primer and a probe and develops a multiple fluorescence RT-PCR detection method used for simultaneously detecting the three animal viruses. The detection method can detect 102 copied plasmids containing target amplification sequences in 1 h. The method has high sensitivity and good specificity, and can achieve rapid high-throughput fluorescence RT-PCR detection for single-virus infection or mixed infection by a plurality of viruses.