Method for inactivating vesicular stomatitis viruses
A technology for vesicular stomatitis and viruses, applied in the field of virus inactivation in the biomedical industry, can solve the problems of low nucleic acid or protein yield, protein denaturation and inactivation, etc., and achieve the effect of simple inactivation method and good inactivation
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Examples
Embodiment 1
[0026] Preparation of 0.04% NaOH-0.003% hemin solution: dissolve hemin in NaOH aqueous solution.
[0027] Take 100 μL of the prepared solution, inoculate Vero cells, incubate at 37°C for 72 hours in a 5% carbon dioxide incubator, and observe Vero cells with a microscope. The results are shown in figure 2 ,from figure 2 It can be seen that the morphology of Vero cells is normal, and the solution of this embodiment has no toxicity to Vero cells.
[0028] Be that 50:1 is added VSV in the solution by the mass ratio of solution and vesicular stomatitis virus (VSV), leave standstill 60min at room temperature, measure virus titer from 6.62lgTCID according to microtitration method 50 down to 3.25lgTCID 50 . 60min virus titer decline trend see Figure 4 .
Embodiment 2
[0030] Preparation of 0.04% NaOH-0.03% hemin solution: dissolve hemin in NaOH aqueous solution.
[0031] Take 100 μL of the prepared solution, inoculate Vero cells, incubate at 37°C for 72 hours in a 5% carbon dioxide incubator, and observe Vero cells with a microscope. The results are shown in image 3 ,from image 3 It can be seen that the morphology of Vero cells is normal, and the solution of this embodiment has no toxicity to Vero cells.
[0032] Be that 50:1 is added VSV in the solution by the mass ratio of solution and vesicular stomatitis virus (VSV), leave standstill 60min at room temperature, measure virus titer from 6.62lgTCID according to microtitration method 50 down to 2.38lgTCID 50 . 60min virus titer decline trend see Figure 5 .
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Description
Claims
Application Information
- IPC
- C12N7/06; C12R1/93
- CPC
- C12N7/00; C12N2760/20263
- Inventors
- 闻洁; 汪涛