Method for inactivating vesicular stomatitis viruses

A technology for vesicular stomatitis and viruses, applied in the field of virus inactivation in the biomedical industry, can solve the problems of low nucleic acid or protein yield, protein denaturation and inactivation, etc., and achieve the effect of simple inactivation method and good inactivation

Active Publication Date: 2020-05-08
苏州良辰生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While the existing chemical methods inactivate the virus, the protein in the sample is also denatured and inactivated to a certain extent, which may result in a low yield of nucleic acid or protein in the sample

Method used

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  • Method for inactivating vesicular stomatitis viruses
  • Method for inactivating vesicular stomatitis viruses
  • Method for inactivating vesicular stomatitis viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of 0.04% NaOH-0.003% hemin solution: dissolve hemin in NaOH aqueous solution.

[0027] Take 100 μL of the prepared solution, inoculate Vero cells, incubate at 37°C for 72 hours in a 5% carbon dioxide incubator, and observe Vero cells with a microscope. The results are shown in figure 2 ,from figure 2 It can be seen that the morphology of Vero cells is normal, and the solution of this embodiment has no toxicity to Vero cells.

[0028] Be that 50:1 is added VSV in the solution by the mass ratio of solution and vesicular stomatitis virus (VSV), leave standstill 60min at room temperature, measure virus titer from 6.62lgTCID according to microtitration method 50 down to 3.25lgTCID 50 . 60min virus titer decline trend see Figure 4 .

Embodiment 2

[0030] Preparation of 0.04% NaOH-0.03% hemin solution: dissolve hemin in NaOH aqueous solution.

[0031] Take 100 μL of the prepared solution, inoculate Vero cells, incubate at 37°C for 72 hours in a 5% carbon dioxide incubator, and observe Vero cells with a microscope. The results are shown in image 3 ,from image 3 It can be seen that the morphology of Vero cells is normal, and the solution of this embodiment has no toxicity to Vero cells.

[0032] Be that 50:1 is added VSV in the solution by the mass ratio of solution and vesicular stomatitis virus (VSV), leave standstill 60min at room temperature, measure virus titer from 6.62lgTCID according to microtitration method 50 down to 2.38lgTCID 50 . 60min virus titer decline trend see Figure 5 .

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Abstract

The invention relates to a method for inactivating vesicular stomatitis viruses. Thus, hematin can contact with vesicular stomatitis viruses. A reagent for inactivating the vesicular stomatitis viruses is also related. The reagent includes alkali, hemin and solvents, wherein the mass percentage concentration of the alkali is 0.01-0.1%, and the feeding mass percentage concentration of the hemin is0.001-0.1%. The reagent has no toxicity to cells and can well inactivate the vesicular stomatitis viruses; and the method is simple and can remove added inactivation reagents without post-processing operation.

Description

technical field [0001] The invention belongs to the field of virus inactivation in the biomedicine industry, and in particular relates to a method for inactivating vesicular stomatitis virus. Background technique [0002] At present, the virus inactivation methods in biochemical drugs mainly include low pH method, ethanol treatment method, heating method and so on. While the existing chemical methods inactivate the virus, the protein in the sample is also denatured and inactivated to a certain extent, which may result in a low yield of nucleic acid or protein in the sample. Contents of the invention [0003] An object of the present invention is to provide a method for inactivating vesicular stomatitis virus which is not toxic to cells. [0004] In order to solve the above technical problems, the present invention adopts the following technical solutions: [0005] One aspect of the present invention provides a method for inactivating vesicular stomatitis virus, which inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/06C12R1/93
CPCC12N7/00C12N2760/20263
Inventor 闻洁汪涛陈弈松曹忆徐林
Owner 苏州良辰生物医药科技有限公司
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