The present invention provides a method for extracting a
mycobacterium genome, and belongs to the field of biological detection technology. The method for extracting the
mycobacterium genome provided by the present invention uses lysase LysA and LysB for wall breaking treatment, which solves the problem of poor
mycobacterium genome extraction effect in the prior art. The total genome amount of
Mycobacterium tuberculosis extracted by the present invention can reach about 5-10 times that of the traditional classical CTAB genome extraction method, and the integrity of the obtained genome fragments is significantly improved. Compared with the use of
lysozyme overnight treatment in the traditional CTAB genome extraction method, the LysA and LysB treatment in the present invention for 2 hours can achieve several times the extraction efficiency, greatly shortening the extraction time, being superior to the traditional CTAB genome extraction method and the genome extraction kit on the market, and being conducive to the rapid and efficient extraction of complete mycobacterium genomes in clinical and laboratory settings.