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Carboxamide compound and application thereof

A compound and carboxylamine technology, applied in the field of drug preparation for preventing and treating animal viral diseases, can solve problems such as failure to effectively control the growth or proliferation of vesicular stomatitis virus, and achieve the advantages of overcoming high cost, preventing proliferation and low biological toxicity. Effect

Active Publication Date: 2019-11-19
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no drugs that effectively control the growth or proliferation of vesicular stomatitis virus

Method used

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  • Carboxamide compound and application thereof
  • Carboxamide compound and application thereof
  • Carboxamide compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Baby hamster kidney cells (BHK21) were cultured in DMEM medium containing 10% fetal calf serum at 37°C and 5% CO 2 When cultured to 90% confluence under ambient conditions, the compound 2-imino-2H-chromene-3-carboxamide was added to the 6-well plate at a dose of 100 μl / well, and the effect of the compound on the growth of BHK21 cells was observed after 24 hours. Figure 4 is the effect of 2-imino-2H-chromene-3-carboxamide on the growth of BHK21 cells, wherein Figure 4 -A is the carboxylamine compound group, Figure 4 -B is a blank control group. From Figure 4 It can be seen that the state of the cells after adding the carboxylamine compound is not significantly different from that of the complete blank group, indicating that the compound has no biological toxicity.

Embodiment 2

[0041] Baby hamster kidney cells (BHK21) were cultured in DMEM medium containing 10% fetal calf serum at 37°C and 5% CO 2 When cultured in the environment until 90% confluence, 20 μl of vesicular stomatitis virus was added to the 6-well plate, and the culture was continued for 2 hours, and then the compound 2-imino-2H-chromene-3- Carboxamide was continued to be cultured in the coexistence environment of poison and drug, and the cytopathic state of BHK21 cells and the proliferation state of virus were observed after 24 hours.

[0042] Figure 5 To intervene the cytopathic effect 24 hours after VSV infects BHK21 with the dose of 5 μ l / well compound, wherein, Figure 5 -A: cell state after coexistence of poison and drug (5 μl / well) for 24 hours, Figure 5 -B: Infected and untreated control, Figure 5 -C: uninfected control group: from Figure 5 It can be seen that after the intervention of low-dose 2-imino-2H-chromene-3-carboxamide, compared with the non-drug-treated control ...

Embodiment 3

[0044] Baby hamster kidney cells (BHK21) were cultured in DMEM medium containing 10% fetal calf serum at 37°C and 5% CO 2 When cultured in the environment until 90% confluence, 20 μl of vesicular stomatitis virus was added to the 6-well plate, and the culture was continued for 2 hours, and then the compound 2-imino-2H-chromene-3- Carboxamide was continued to be cultured in the coexistence environment of poison and drug, and the cytopathic state of BHK21 cells and the proliferation state of virus were observed after 24 hours.

[0045] Figure 7 In order to intervene the cytopathic effect 24 hours after VSV infects BHK21 with the dose of 20 μ l / well compound, wherein, Figure 7 -A: cell state after coexistence of poison and drug (5 μl / well) for 24 hours, Figure 7 -B: Infected and untreated control, Figure 7 -C: uninfected control group: from Figure 7 It can be seen that after the intervention of a medium dose of 2-imino-2H-chromene-3-carboxamide, compared with the non-dru...

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Abstract

The invention relates to a carboxamide compound and application thereof, belonging to the technical field of preparation of drugs used for preventing and treating animal viral epidemic diseases. The carboxamide compound disclosed by the invention is 2-imino-2H-chromene-3-carboxamide, and the structural formula of the carboxamide compound is shown in the specification. The carboxamide compound disclosed by the invention has no obvious biotoxicity, can effectively control the proliferation of vesicular stomatitis viruses in BHK21 cells at a low dose, delays the occurrence of cytopathic effect, is capable of completely blocking the proliferation of viruses at a large dose, wins over a time window for the prevention and control of epidemic diseases caused by the vesicular stomatitis viruses, and provides a new drug for the treatment of affected individuals.

Description

technical field [0001] The invention relates to the technical field of preparation of medicaments for preventing and treating animal viral diseases, in particular to a carboxylamine compound and its application in the preparation of medicaments for intervening or controlling the growth or proliferation of vesicular stomatitis virus. Background technique [0002] Vesicular stomatitis is an acute, febrile, zoonotic infectious disease caused by vesicular stomatitis virus. The pathogen VSV virus is a member of Rhabdoviridae and Vesiculovirus, and the virus particles are bullet-shaped or cylindrical, with a size of about 150-180nm×50-70nm. There is an envelope structure outside the nucleocapsid of VSV virus, and there are evenly and densely distributed fibrils with a length of about 10 nm on the envelope. The genome is single-stranded negative-strand RNA, encoding five main structural proteins: N, NS, M, G, and L. The nucleocapsid of VSV virus has a helical symmetrical structur...

Claims

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Application Information

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IPC IPC(8): C07D311/58A61P31/14
CPCA61P31/14C07D311/58
Inventor 李志勇李爱欣解银丽杨建设韩立闯卢曾军刘在新秦晓东张志东
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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