44 results about "Untreated control" patented technology

A method of preparing a tobacco material for use in a smoking article is provided, including (i) mixing a tobacco material, water, and an additive selected from the group consisting of lysine, glycine, histidine, alanine, methionine, glutamic acid, aspartic acid, proline, phenylalanine, valine, arginine, di- and trivalent cations, asparaginase, saccharides, phenolic compounds, reducing agents, compounds having a free thiol group, oxidizing agents, oxidation catalysts, plant extracts, and combinations thereof; (ii) heating the mixture; and (iii) incorporating the heat-treated mixture into a smoking article as a smokable material. A smoking article in the form of a cigarette is also provided that includes a tobacco material pre-treated to inhibit reaction of asparagine to form acrylamide in mainstream smoke. Upon smoking, the smoking article is characterized by an acrylamide content of mainstream smoke that is reduced relative to an untreated control smoking article.

A germicidal surface-covering assembly that includes at least two different donnable or drapable garments. Each garment defines at least one treated surface that is susceptible to pathogen contamination in a physical contamination event when used as intended in an environment subject to contamination (e.g., a clinical environment, a laboratory or a workplace). Each treated surface is adapted to provide a time-dependent reduction in the number of pathogens available at that treated surface after a physical contamination event, such that at least a predetermined time after a physical contamination event at a first location on a first treated surface of a first garment, a first physical contact between the first location on the first treated surface and a second location on a second treated surface of a second garment results fewer viable pathogens on the second treated surface as compared to an untreated control. Moreover, at least a predetermined time after the first physical contact, a second physical contact between the second location on the second treated surface and a third location on a third treated surface of a third garment results in fewer viable pathogens on the third treated surface as compared to an untreated control.

A method of preparing a tobacco material for use in a smoking article is provided, including (i) mixing a tobacco material, water, and an additive selected from the group consisting of lysine, glycine, histidine, alanine, methionine, glutamic acid, aspartic acid, proline, phenylalanine, valine, arginine, di- and trivalent cations, asparaginase, saccharides, phenolic compounds, reducing agents, compounds having a free thiol group, oxidizing agents, oxidation catalysts, plant extracts, and combinations thereof; (ii) heating the mixture; and (iii) incorporating the heat-treated mixture into a smoking article as a smokable material. A smoking article in the form of a cigarette is also provided that includes a tobacco material pre-treated to inhibit reaction of asparagine to form acrylamide in mainstream smoke. Upon smoking, the smoking article is characterized by an acrylamide content of mainstream smoke that is reduced relative to an untreated control smoking article.

The subject invention concerns materials and methods for evaluating the susceptibility of bacterial cells to an antibiotic or other antimicrobial compound or agent. In one embodiment, a sample comprising a microbial population is exposed to an antibiotic of interest. The sample is then processed using FISH-RiboSyn methods to determine the specific growth rate of the antibiotic-exposed microbes as compared to an untreated control. The subject invention also concerns materials and methods for determining the most suitable and / or effective antibacterial treatment for a person or animal having a bacterial infection.

The invention discloses a white spirit aging accelerating agent, an aging hastening method and an aging tank. The white spirit aging accelerating agent is prepared by steps as follows: a fibrous ferric loaded attapulgite based aging accelerating assistant and attapulgite are mixed, pelleted and calcinated to prepare 10-20-mesh particles as the white spirit aging accelerating agent for industrial production application; the aging accelerating agent and liquor in certain mass-volume ratio are arranged in the aging tank provided with a jacket, hot water is introduced into the jacket to guarantee the aging temperature, intermittent stirring and ventilation are performed, and aging is performed for certain time. The aging accelerating agent is safe, is free of toxic and side effects and can be recycled; the aging accelerating process is simple, and conditions are easy to control; 20 d of strong-flavor white spirits processed with the method is equivalent to white spirits stored for 1 year under the common production conditions, and chromatograph skeleton components of the white spirits are remarkably different from those of unprocessed contrast white spirits; and meanwhile, according to sensory evaluation of a wine taster, the processed white spirits have remarkable stale flavor, well-balanced wine body, more mellow and thick soft-sweet taste and more refreshing aftertaste.

Disclosed are methods of polarizing macrophages to exhibit M2 phenotype, including introducing an effective amount of gingiva-derived mesenchymal stem cells to an environment comprising a population of macrophages such that the macrophages are in fluid communication with the gingiva-derived mesenchymal stem cells. Also disclosed are methods of promoting cutaneous wound healing including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells, thereby resulting in at least one of accelerated wound closure, rapid re-epithelialization, improved angiogenesis and improved tissue remodeling relative to untreated controls. Also disclosed are methods for attenuating contact hypersensitivity in a patient, the methods including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells at a time at least timeframe selected from the group consisting of before sensitization, after sensitization and before challenge and after challenge, thereby attenuating contact hypersensitity.

Methods of treating liver disease in a subject, including administering to the subject an effective amount of one or more modified green tea polyphenols to reduce, decrease, limit or prevent one or more symptoms of liver disease relative to an untreated control subject are provided. In a preferred embodiment the one or more modified green tea polyphenols are administered at a dose of 400 mg / kg body weight five times weekly. In some embodiments the disclosed methods further include administering to the subject one or more additional pharmaceutically active agents. In one embodiment the one or more additional pharmaceutically active agents is a chemotherapeutic agent.

The invention discloses an aging accelerating method on the basis of baijiu aging accelerating agents. The aging accelerating method includes arranging the aging accelerating agents and baijiu liquid in an aging tank with a jacket according to certain mass and volume ratios, filling the jacket with hot water to guarantee the aging temperatures and intermittently carrying out stirring, gas filling and aging on the baijiu liquid for a certain period of time; mixing fibrous iron-loaded attapulgite-based aging accelerating auxiliaries and attapulgite with one another to obtain mixtures, and granulating and calcining the mixtures to obtain granules with the sizes of 10-20 meshes. The granules are used as the baijiu aging accelerating agents applied to industrial production. The aging accelerating method has the advantages that the baijiu aging accelerating agents are safe, are free of toxic and side effects and can be reused; aging accelerating processes are simple, and conditions are easy to control; the level of strong-flavor baijiu treated by the aid of the aging accelerating method for 20 d is equivalent to the level of baijiu which is stored under usual production conditions for approximately 1 year, and chromatography framework ingredients of the strong-flavor baijiu are obviously different from chromatography framework ingredients of untreated comparison baijiu; as shown by sensory evaluation of baijiu tasters, obvious age flavor effects can be realized by the treated baijiu, a baijiu body of the baijiu is harmonious, and the baijiu is soft, sweet and mellow and has clean and cool aftertaste.

This invention describes discovery and development of a biological system of plant growth promotion and environmental improvement by carbon sequestration and / or nitrogen utilization by application of a microbial agent, e.g., a highly effective strain of T. viride, particularly strains NRRL B-50520 and / or K5. This strain outperforms the best current strains of Trichoderma used for this purpose available commercially. The highly active products also are expected to increase plant productivity and improve quality of fruits, vegetables, flowers or other plant products. The invention also describes and demonstrates that strain combinations including Bacillus amyloliquifaciens AS2 or AS3, as well as disclosed metabolites, promote plant growth. This is true across monocots and dicots, seed treatments and foliar sprays. In the field plant height, shoot weight, root weight, and photosynthesis (Spad meter readings) were all increased in comparison to the untreated control as well as the leading biological seed treatments. The invention also describes metabolite utilization for enhancing plant growth.

The present invention discloses a method for producing a commercial non-GM tomato crop exhibiting at least one inheritable improved trait by exposing said population of plants to a predetermined light treatment regime, monitoring at least one trait of said tomato plants comparing to said control population and selecting the plants having the best improved trait.The tomatoes obtained by the method of this invention exhibit an improved room temperature (17-27° C.) shelf life of at least 45 days after harvesting, as compared to about 10 days for untreated control tomatoes.

Methods of treating liver disease in a subject, including administering to the subject an effective amount of one or more modified green tea polyphenols to reduce, decrease, limit or prevent one or more symptoms of liver disease relative to an untreated control subject are provided. In a preferred embodiment the one or more modified green tea polyphenols are administered at a dose of 400 mg / kg body weight five times weekly. In some embodiments the disclosed methods further include administering to the subject one or more additional pharmaceutically active agents. In one embodiment the one or more additional pharmaceutically active agents is a chemotherapeutic agent.

The invention discloses a physical sterilization, disinfection and preservation method by utilizing two-way three-layer butterfly-type five-section composite preserved fresh-cut lily. The temperature is manually and automatically controlled, the inert gases are pressurized, and the lily raw materials are subjected to dormance treatment, so that the mildew rate of the fresh lily raw materials is reduced, the inherent scented, crispy, smooth, delicious, original ecological and pure natural characteristics and white color of the fresh lily are maintained, and the treatment cost is reduced. Moreover, the fresh-cut lily treated by the method has the characteristics that the nutritional ingredients are not lost, the original appearance is kept, the inherent scented, crispy, smooth, delicious and raw white like jades characteristics of the lily are maintained, and the storage period is prolonged, and compared with an untreated control sample, the method has the advantage that the preservation and storage period can be prolonged by over 5 times.

The invention discloses a spice prepared by carrying out microbial fermentation on walnut diaphragma juglandis and used for cigarettes, and applications thereof. The preparation method comprises: using walnut diaphragma juglandis as a raw material, carrying out amplification culture on Kineococcus cavernae SYSUZL-1, fermenting the walnut diaphragma juglandis by using the cultured Kineococcus cavernae SYSUZL-1, carrying out shake culture for 24-48 h at a temperature of 30 DEG C, carrying out reflux extraction, filtering to remove the residue, and carrying out pressure reducing concentration on the supernatant to obtain the spice for cigarettes, wherein the preservation number of the Kineococcus cavernae SYSUZL-1 is CCTCC M2016738, and the microbiological characteristic analysis results and the 16S rRNA gene sequence analysis results show that the Kineococcus cavernae SYSUZL-1 is the new microorganism species. Compared to the untreated control, the product obtained through the co-fermentation of the Kineococcus cavernae SYSUZL-1 and the walnut diaphragma juglandis has the following advantages that the special acid aroma is increased while the original characteristic aroma of the walnut is maintained, the richness of the aroma is increased, and the irritation to the larynx is decreased.

A product comprising a natural acellular xenogenic vascular tissue matrix having at least an 80% reduction in DNA content as compared to an untreated control vascular tissue matrix and being antigenically inert by being substantially free of epitopes capable of reacting with pre-formed human antibodies and also without having the ability to substantially activate complement. The invention also includes methods of preparing such products and uses of the products especially in bypass surgery.

The invention discloses a method for treating seeds for promoting germination of oilseed rape and improving the cold resistance of the oilseed rape. The method is characterized by comprising steps of 1), preparing the seeds, to be more specific, preparing the oilseed rape seeds to be sown during production and conventionally screening the seeds for standby application; 2), treating the selected oilseed rape seeds by the aid of chemical agents, to be more specific, soaking the selected oilseed rape seeds in 0.05% H2O2 solution at the room temperature of 20-25 DEG C for 4-8h or at the room temperature of 10-15 DEG C for 8-12h, and then repeatedly washing the oilseed rape seeds by the aid of tap water; 3), dehydrating and drying the seeds, to be more specific, spreading and drying the treated seeds in shade places under a room-temperature condition for 1-5 days, and storing the seeds in sealed bags for standby application after the water contents of the seeds are reduced and reach the water contents of the seeds in pretreatment states. The method has the advantages that as proved by tests, germination rates and germination indexes of the oilseed rape seeds treated by the aid of the method are higher than germination rates and germination indexes of untreated control group, and the oilseed rape is high in growth speed after the seeds are germinated and can grow robustly.

A method for suppressing the formation of ammonia comprising providing a carrier material to a container having a headspace; providing a bacteria, an acidifier and an odor inhibitor to the carrier material, the bacteria comprising Staphylococcus-xylosus or Staphylococcus-cohnii bacteria, and the odor inhibitor comprising a salt of an aminopolycarboxylic acid compound; and applying animal waste tothe carrier material; wherein there is a 5 to 98 percent improvement of ammonia content in the headspace as compared to an untreated control comprising a container containing the carrier material andthe bacteria and not contain the odor inhibitor.

The invention provides application of melatonin in enhancing aroma of Shine-Muscat grape fruits and belongs to the technical field of plant growth regulation. According to the application, the melatonin can significantly enhance the aroma of Shine-Muscat grape fruits. After application, compared with untreated controls, the total quantity of aromatic substances of fruits is increased by 4 times orabove after melatonin treatment, the type of aromatic substances of fruits after melatonin application is 1.65 times that of control fruits, the total quantity of aromatic substances is 4.32 times that of the controls, the aromatic substances with Muscat characteristics are increased by 4 times or above, and the characteristic aromatic substance with the highest content is 2-Hexenal, (E)-, whichis 4.54 times that of the controls. The melatonin treatment can increase the contents of alcohols, aldehydes, terpenes and other aromatic substances.

The invention belongs to the technical field of biological medicines, and discloses an MSTN nano antibody, a construction method and application thereof. Constructing a nano antibody library of sheepMSTN gene expression product; obtaining a specific nano antibody aiming at an MSTN gene expression product through a phage display technology; and constructing a bivalent sheep MSTN nano antibody; theMSTN nano antibody has the functions of increasing animal muscle content and reducing animal fat content on mice. When the weight gain was mainly muscle weight gain, the sheep MSTN nano antibody showed that the weight gain was higher than that of untreated control group; mice showed that the muscle weight of the nano antibody group was significantly higher than that of the control group, and thepercentage of muscle in the whole body was also significantly higher than that of the control group. Sheep MSTN nano antibodies can promote muscle growth in animals.

The invention discloses a white spirit aging accelerating agent, an aging hastening method and an aging tank. The white spirit aging accelerating agent is prepared by steps as follows: a fibrous ferric loaded attapulgite based aging accelerating assistant and attapulgite are mixed, pelleted and calcinated to prepare 10-20-mesh particles as the white spirit aging accelerating agent for industrial production application; the aging accelerating agent and liquor in certain mass-volume ratio are arranged in the aging tank provided with a jacket, hot water is introduced into the jacket to guarantee the aging temperature, intermittent stirring and ventilation are performed, and aging is performed for certain time. The aging accelerating agent is safe, is free of toxic and side effects and can be recycled; the aging accelerating process is simple, and conditions are easy to control; 20 d of strong-flavor white spirits processed with the method is equivalent to white spirits stored for 1 year under the common production conditions, and chromatograph skeleton components of the white spirits are remarkably different from those of unprocessed contrast white spirits; and meanwhile, according to sensory evaluation of a wine taster, the processed white spirits have remarkable stale flavor, well-balanced wine body, more mellow and thick soft-sweet taste and more refreshing aftertaste.

The invention discloses a cultivation method of sympodial bamboos used for producing high-cellulose-content fibers. The cultivation method of the sympodial bamboos used for producing the high-cellulose-content fibers comprises the following steps: preparing a compound solution used for adjusting and controlling a cellulose content of the sympodial bamboos, wherein the compound solution comprises the following components: polypeptide urea with a concentration of 5-20 mg / L, a gibberellic acid (GA3) with a concentration of 5-15 mg / L, 2-diethylaminoethyl hexanoate with a concentration of 15-25 mL / L, potassium dihydrogen phosphate with a concentration of 80-120 mg / L, Zn(NO3)2.EDTA with a concentration of 8-12 mg / L, sodium molybdate with a concentration of 0.1-0.2 mg / L and MgSO4.7H2O with a concentration of 30-60 mg / L, and a pH value of the compound solution is 6-6.5; adjusting the basic soil fertility through fertilization; and planting 40 sympodial bamboos per mu according to spacing in rows and spacing between the rows of 4*4 m, and keeping 10-15 annual or biennial bamboos of each sympodial bamboo. According to the cultivation method of the sympodial bamboos used for producing the high-cellulose-content fibers, cultivation of a sympodial bamboo raw material used for high-quality fibers is regarded as the purpose, operation is simple, the bamboos grow fast, the bamboo cellulose content is high, the cellulose content of the biennial bamboos is, on average, 14.1% higher than that of untreated controls, and directional cultivation of a bamboo forest used for the high-quality fibers can be achieved.

The invention discloses a cigarette spice prepared by microbiological fermentation of prunella vulgaris and application of the cigarette spice. The cigarette spice is obtained by taking the prunella vulgaris as a raw material, performing amplification culture on lysobacter C8-1 to ferment the prunella vulgaris, performing shake culture for 24-48h at 30 DEG C, and then performing reflux extraction,slag removal by filtration and vacuum concentration of supernatant liquid. The preservation number of the strain is CGMCC 1.16271. Microbiological characteristic analysis and 16S rRNA gene sequence analysis show that the lysobacter is a new microbiological strain. Compared with untreated controls, the product obtained by co-fermentation of the lysobacter C8-1 and the prunella vulgaris has the advantages that under the condition of keeping the original characteristics and smell of the prunella vulgaris, the bitter taste is reduced, and the effect of promoting the secretion of saliva or body fluid is obvious.

Disclosed are methods of polarizing macrophages to exhibit M2 phenotype, including introducing an effective amount of gingiva-derived mesenchymal stem cells to an environment comprising a population of macrophages such that the macrophages are in fluid communication with the gingiva-derived mesenchymal stem cells. Also disclosed are methods of promoting cutaneous wound healing including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells, thereby resulting in at least one of accelerated wound closure, rapid re-epithelialization, improved angiogenesis and improved tissue remodeling relative to untreated controls. Also disclosed are methods for attenuating contact hypersensitivity in a patient, the methods including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells at a time at least timeframe selected from the group consisting of before sensitization, after sensitization and before challenge and after challenge, thereby attenuating contact hypersensitivity.

The present invention provides compositions and methods for effective treatment of a lysosomal acid lipase deficiency (LALD) disease, in particular, Wolman's disease and Cholesteryl Ester Storage Disease (CESD). Among other things, the present invention provides a method of treating a lysosomal acid lipase deficiency (LALD) disease, including administering to an individual suffering from or susceptible to the LALD disease a therapeutic effective amount of a lysosomal acid lipase periodically at an administration interval such that lipid level in liver, spleen and / or small intestine is reduced by at least 20% as compared to an untreated control.

A product comprising a natural acellular xenogenic vascular tissue matrix having at least an 80% reduction in DNA content as compared to an untreated control vascular tissue matrix and being antigenically inert by being substantially free of epitopes capable of reacting with pre-formed human antibodies and also without having the ability to substantially activate complement. The invention also includes methods of preparing such products and uses of the products especially in bypass surgery.

The present invention relates to a method for reducing wetwood content in green lumber by a treatment with a selected fungal species, such as Phlebiopsis gigantea or Gliocladium roseum, prior to kiln drying. In the laboratory conditions, the biological treated boards reduced wood moisture content (MC) by 22-37% more than untreated boards. The time required for drying biological treated boards was approximately reduced by 10% compared with untreated controls, and by 33% compared with freshly-saw lumber. After drying, the biological treated boards reduced the total deformation with stain, warping and checking by 14-25%.

The present disclosure relates to a seed of a plant, a seedling or a plant derived from the seed containing an increased internal concentration of glycine-betaine compared to untreated controls. The present disclosure further relates to a method to produce a seed of a plant, a seedling or a plant derived from said seed containing an increased internal concentration of glycine-betaine which will confer to the seed, seedling or plant derived from said seed an enhanced germination potential and / or increased tolerance against various environmental stresses compared to untreated controls. The present disclosure further relates to uses of glycine-betaine for enhancing the germination potential of a seed and / or for increasing the tolerance of a seed against environmental stress. The present disclosure also relates to seedlings and plants, or parts thereof, derived from a seed of the present invention having an increased international concentration of glycine-betaine. The present disclosure further relates to seedlings or plants, or parts thereof, obtainable by the methods of the present invention.

Here we show that SEG / SEI presented from a HLA-DQ8 (HLA-DQB*0302 and HLA-DQA*0301) platform prevent the de novo outgrowth (vaccination) of Lewis lung carcinoma (LLC) and B16-F10 melanoma and retard the growth of established tumors with no significant toxicity. Vaccination of DQ8 tg mice with irradiated LLC or B16-F10 melanoma followed by SEG / SEI immunization and live tumor challenge resulted in 100 and 66% survival respectively for 200 days compared to a median survival of 20 days for untreated controls (p<0.001). In vaccination studies, DQ8 tg mice showed a surge in IFNγ serum levels reaching 3000 fold above baseline devoid of a parallel spike in TNFα levels above baseline levels. Presentation of the SEG / SEI superantigen from a MHC-DQ8 platform, therefore, augments the therapeutic index of these SAgs inducing a tumoricidal response against Lewis lung carcinoma and B16 melanoma accompanied by a sharp increase of therapeutic IFNγ levels absent toxic levels of TNFα.

Here we show that SEG / SEI presented from a HLA-DQ8 (HLA-DQB*0302 and HLA-DQA*0301) platform prevent the de novo outgrowth (vaccination) of Lewis lung carcinoma (LLC) and B16-F10 melanoma and retard the growth of established tumors with no significant toxicity. Vaccination of DQ8 tg mice with irradiated LLC or B16-F10 melanoma followed by SEG / SEI immunization and live tumor challenge resulted in 100 and 66% survival respectively for 200 days compared to a median survival of 20 days for untreated controls (p<0.001). In vaccination studies, DQ8 tg mice showed a surge in IFNγ serum levels reaching 3000 fold above baseline devoid of a parallel spike in TNFα levels above baseline levels. Presentation of the SEG / SEI superantigen from a MHC-DQ8 platform, therefore, augments the therapeutic index of these SAgs inducing a tumoricidal response against Lewis lung carcinoma and B16 melanoma accompanied by a sharp increase of therapeutic IFNγ levels absent toxic levels of TNFα.

The invention discloses a tobacco spice prepared by microbial fermentation of Elatagnus conteta Roxb. fruits and application thereof. The tobacco spice is prepared by: taking Elatagnus conteta Roxb. fruits as the raw materials, subjecting Blastococcus cavernae sp. SYSUZL-2 to enlarged culture, then fermenting Elatagnus conteta Roxb. fruits, conducting shaking culture at 30DEG C for 24-48h, and then carrying out reflux extraction, filtration and deslagging, and supernatant vacuum concentration. The strain has a preservation number of CCTCC M2016739. Microbiological characteristic analysis and 16S rRNA gene sequence analysis indicate that the Blastococcus cavernae sp. is a new microbial species. Compared with an untreated control product, the strain and Elatagnus conteta Roxb. fruit co-fermentation product can endow the prepared cigarettes with increased sweetness, the liquid engendering effect is obvious, the smoke is soft and fine, and the aroma richness is increased.

The invention discloses an application of NaHS as a H2S donor to inhibition of abscission of plant organs. H2S gas prepared from a NaHS liquid by volatilization is used for fumigating arabidopsis thaliana and tomato plants, then abscission of flower organs and capsule cracking of arabidopsis thaliana can be delayed remarkably, and pedicel abscission of the tomatoes can be inhibited remarkably; an acceleration action of an abscission accelerator ethylene on abscission can be weakened by H2S; the expression level of marker protein (polygalacturonase, cellulase and cell wall expansion protein) encoding genes in an organ abscission process is detected in an H2S treating process, a result proves that the expression level is different from that of an untreated control group, and the expression quantity of related genes in pedicels treated with H2S rises slowly, which means H2S inhibits the abscission process on the molecular level. A reagent is single and easily available, the operation is simple, and one practical and effective method is provided for delaying abscission of the plant organs.