50 results about "Post transplant" patented technology

An isolated cell having a central memory T-lymphocyte (Tcm) phenotype, the cell being tolerance-inducing cell and capable of homing to the lymph nodes following transplantation, the cell being transduced to express a cell surface receptor comprising a T cell receptor signaling module is described. Methods of generating same and using same are also described.

The invention belongs to the technical field of medical equipment, and particularly discloses a reversible electroporation combined medical kit for organ transplantation and transfer and application of the reversible electroporation combined medical kit. According to the medical kit for organ transplantation, a novel electric pulse device is additionally designed on the basis of original design, a large amount of emergency response generated after organ transplantation can be remarkably reduced, and the survival rate of organs is increased. The novel electric pulse device is designed to be attached to the inner wall of the box body, the designed electric pulse electrode plate is attached to the buffer pad, the buffer pad is made of an insulating non-toxic heat preservation material, and organ preservation liquid is contained in the organ preservation bag. According to the medical kit for organ transplantation, the additional electrodes can be used for applying electric pulses to organs in the organ storage bag, medicine in the organ storage liquid is promoted to enter the organs, meanwhile, the optimal applied pulse intensity is confirmed in combination with cell experiments, the potential distribution condition is simulated and confirmed in combination with simulation software, and emergency reaction generated after perfusion can be reduced; therefore, normal organ functions are ensured, and the success rate of transplantation is improved.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention discloses a mesenchymal stem cell preparation and application thereof, and belongs to the technical field of cell biology. The mesenchymal stem cell preparation is a mesenchymal stem cell pretreated by a combined drug of ziyuglycoside II and shikonin. Through drug pretreatment, the antioxidant capacity of the mesenchymal stem cells is synergistically enhanced, the survival rate of the cells after transplantation is enhanced, the treatment effect of the cells on the NAFLD hepatocytes is remarkably enhanced, and lipid accumulation in the hepatocytes is effectively reduced. Therefore, the invention provides a novel method for enhancing the treatment capacity of the mesenchymal stem cells NAFLD, the method is suitable for bone marrow and umbilical cord mesenchymal stem cells, and an efficient and reliable preparation and an optimization scheme are provided for clinical stem cell treatment of the NAFLD.

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

Nanoparticles, methods, and kits are provided for supplying nutrients and other therapeutic agents to transplanted cells. Nutrient deprivation is a significant factor which contributes to poor outcome of many cell transplants because cells receive insufficient nutrients until they are able to establish a functional microcirculation to support their metabolic and physiological needs after transplantation. Nanoparticles are provided for use in supplying nutrients and other therapeutic agents to transplanted cells to improve cell survival. Such nanoparticles can be used to supply nutrients and other factors to transplanted cells until the transplanted cells are able to develop a new microcirculation.

The invention relates to the technical field of greening transplanting, and discloses a road greening disc transplanting and maintaining all-in-one machine based on a soil moisturizing structure, the road greening disc transplanting and maintaining all-in-one machine comprises a supporting frame, a mounting frame and a base plate, the mounting frame and the base plate are arranged on the supporting frame, a transplanting mechanism is arranged between the mounting frame and the base plate, an auxiliary assembly is arranged on the base plate, and the auxiliary assembly comprises a maintaining part and a moisturizing part. In the working process, maintenance and moisture preservation work of transplanted green plants is achieved. The road greening disc transplanting and maintaining all-in-one machine based on the soil moisturizing structure can effectively solve the problems that in the prior art, when facing a large-scale green belt reconstruction project, the whole process depends on manual digging and carrying, a large amount of manpower needs to be consumed, meanwhile, due to the fact that the efficiency is low, the whole transplanting period is remarkably prolonged, and some small green plants are difficult to transplant in the transplanting process. In the prior art, the root system is exposed in the air for a long time, and physiological damage is easily caused by excessive water loss, so that the transplanted green plant is directly poor in growth.

This invention discloses a co-culture immune isolation device after cell transplantation, belonging to the technical field of cell culture devices. The device includes a culture plate with several first cavities inside and placement slots outside the first cavities. A cover plate is provided above the culture plate, and several second cavities are provided on the cover plate. A sealing ring is provided on the outside of the second cavities, and a liquid inlet mechanism is provided inside the cover plate, connected to the second cavities. This invention uses the above-mentioned co-culture immune isolation device after cell transplantation to achieve co-culture of multiple cells by setting multiple independent first cavities, preventing cell migration or direct contact and avoiding immune rejection. The protective cover and liquid inlet mechanism achieve closed-loop automatic liquid inlet, continuously diffusing signal molecules into the first cavities to maintain co-culture homeostasis and achieve co-culture of multiple cells.

The present disclosure is generally directed to the use of biomaterial scaffolds engineered with SA-FasL for the transplantation of stem cell derived β-cells as a treatment for Type I diabetes. Early engraftment post-transplantation and subsequent maturation of these β-cells may be limited by the initial inflammatory response, which impacts the ability to sustain normoglycemia at long times. The survival and development of immature hPSC-derived β-cells transplanted on poly(lactide-co-glycolide) (PLG) microporous scaffolds into the peritoneal fat, a site being considered for clinical translation, was investigated. The scaffolds were modified with biotin for binding of a streptavidin-FasL (SAFasL) chimeric protein to modulate the local inflammatory microenvironment. The presence of FasL impacted infiltration of monocytes and neutrophils and altered their phenotypic response. Conditioned media generated from scaffolds explanted at day 4 did not impact hPSC-derived β-cell survival and maturation in vitro, which was not observed with unmodified scaffolds. Following transplantation, β-cell viability and differentiation were improved with SA-FasL modification. A sustained increase in insulin positive cell ratio was observed with SA-FasL modified relative to unmodified scaffolds. These results demonstrate that SA-FasL-modified scaffolds can mitigate initial inflammatory response and enhance β-cell engraftment and differentiation.

The present invention relates to the field of immunotherapy, in particular to diseases associated with Epstein-Barr virus (EBV, also known as human gamma herpesvirus 4), such as cancer or post-transplant lymphoproliferative disease, in particular to adoptive T cell therapy or T cell receptor (TCR) gene therapy. The present invention provides a combination of nucleic acids encoding at least two TCR constructs or corresponding proteins or host cells, wherein each TCR construct is capable of specifically binding to its respective epitope in the context of a respective MHC I, and wherein the epitopes are derived from different antigens expressed by the same infectious agent or cancer, such as EBV antigens. The present invention further provides specific nucleic acids encoding a TCR alpha chain construct (TRA) and / or a TCR beta chain construct (TRB) of a TCR construct specific for an epitope complexed with human MHC I, wherein the epitope is an epitope of an Epstein-Barr virus protein, the TCR construct being specific for an epitope from LMP2A, LMP1 or EBNA3C. Proteins encoded by said nucleic acids, corresponding host cells, as well as pharmaceutical compositions and kits are also an object of the present invention.

The present invention provides compositions and methods for promoting the engraftment of exogenous hematopoietic stem cell grafts and for maintaining the pluripotency and hematopoietic function of these cells after transplantation. [Solution] The present invention provides compositions and methods useful for the depletion of cells such as CD45+, CD135+, CD34+, CD90+, and / or CD110+ cells, and, in particular, for the treatment of various hematopoietic disorders, metabolic disorders, cancer, and autoimmune diseases. For example, antibodies, their antigen-binding fragments, ligands, and conjugates that can be applied to achieve the treatment of these conditions by depleting populations of D45+, CD135+, CD34+, CD90+, or CD110+ cells in a patient such as a human are described herein.

The present disclosure provides engineered T cells with reduced expression or knock out of an immunophilin, and which express a chimeric antigen receptor (CAR) and / or comprise one or more modifications that disrupt MHC class I and / or MHC class II alleles and increase expression of a tolerogenic factor. The present disclosure also provides methods of treating a subject having or suspected of having a post-transplant disorder by administering T cells with reduced expression or knock out of an immunophilin. Also disclosed are pharmaceutical compositions comprising T cells with reduced expression of an immunophilin, for use in treating a subject having or suspected of having a post-transplant disorder.

The invention belongs to the field of bioelectromagnetism, and particularly discloses a method for improving functions of frozen and thawed ovarian tissues by adopting an mT-level pulsed magnetic field. According to the method, ovarian tissue which is subjected to vitrification freezing and resuscitation is exposed in a pulsed magnetic field with specific parameters for intervention treatment; wherein the intensity of the pulsed magnetic field is 5 mT, the current period is 1000-6000 [mu] s, the duty ratio is 50%, the pulse repetition number is 30, and the pulse interval is 60 ms. According to the application, a non-intrusive and drug-residue-free physical intervention means is adopted, inflammatory response can be effectively inhibited, oxidative stress injury can be relieved, the number of functional mitochondria is increased by up-regulating mitochondrial fusion genes (such as Mfn1 and Mfn2), the energy metabolism state of cells is fundamentally improved, the survival rate of transplanted ovarian tissue is remarkably increased, the functional life of the ovarian tissue is prolonged, and the survival rate of the ovarian tissue is increased. The method is simple to operate, low in cost and good in clinical application prospect.

The invention discloses a multi-phase CT image-based early recurrence risk prediction method and system after hepatocellular carcinoma liver transplantation, and belongs to the technical field of medical image processing and artificial intelligence. The method comprises the following steps: S1, multi-phase CT image acquisition and standardization preprocessing; s2, sketching a tumor area and a peritumor area to form a target volume; s3, extracting radiomics features and deep learning features; s4, performing multiple rounds of feature screening, and combining clinical risk factors to construct a deep learning radiomics column graph prediction model; s5, risk prediction and layering; and S6, combining with the traditional clinical standard to realize increment value integration. The system comprises an image access and preprocessing module, a feature calculation engine, a model management module, a risk assessment module and a clinical decision support module. The problems of insufficient information utilization, difficulty in clinical integration and the like in the prior art are solved by jointly analyzing the tumor and the peritumoral region and fusing multi-dimensional features, the early recurrence prediction precision is improved, and support is provided for clinical decision making.

The present invention relates to glycyrrhizin-glycol chitosan conjugate-coated nanoparticles, islet cells, prepared using same, for transplantation, and an MRI imaging composition comprising same. If transplanted, the islet cells comprising the nanoparticles can suppress a post-transplantation immune response. The present invention can provide islet cells for transplantation that can be transplanted to a certain region by magnetic force induction and can be tracked by MRI.

The application relates to the field of biological medicine, and particularly relates to a cold storage solution and application of a combined traditional Chinese medicine compound biological stent drug delivery system in eardrum repair. The cold storage solution comprises a basic solution and L-alanyl-L-glutamine; the basic solution comprises a STEEN solution. The cold storage solution can promote metabolism and synthesis of glutathione of fat grafts, regulate ROS mitochondrial oxidative stress, reduce cold storage damage of the fat grafts, and improve the cold storage quality of the fat grafts. Meanwhile, the application uses Shengji Yuhong collagen sponge which contains an important classical prescription for promoting repair, forms a functional biological material with supporting and pro-angiogenic effects, forms a linkage mode of "double-stage regulation before and after transplantation", closely combines the actual difficulties of cell damage of fat groups and reduction of repair success rate caused by long eardrum repair time in the clinic, explores a brand-new research mode for improving the eardrum repair scheme, and has far-reaching clinical significance.

The application provides a gene set and kit for evaluating the curative effect after allogeneic hematopoietic stem cell transplantation of hematopathy, and belongs to the field of gene detection. The application develops a new post-transplantation curative effect evaluation panel detection design method. The panel comprises: 1. single nucleotide polymorphism (SNP) sites characteristic of individual genetic characteristics, which can be used for graft implantation evaluation; 2. high-frequency mutation sites of blood tumor hotspot genes, which can be used for MRD detection and disease state evaluation; and 3. HLA gene characteristic SNP sites on the short arm of human chromosome 6, which can be used for HLA gene typing, combined with the state of graft implantation, to analyze the occurrence of HLA-loss. The technical scheme of the application can solve the above-mentioned multiple contents of post-transplantation curative effect evaluation at one time, is more accurate and sensitive than the previous detection method, and can greatly reduce the detection cost and shorten the clinical report time.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for treating repeated planting failure and a preparation method and application thereof. The traditional Chinese medicine composition disclosed by the invention has the effects of removing blood stasis, tonifying kidney and warming meridians by promoting blood circulation, removing blood stasis and tonifying kidney; furthermore, the uterus-warming and kidney-tonifying treatment traditional Chinese medicine composition is used for warming meridians and uterus, tonifying kidney and nourishing blood to improve the intimal receptivity; further, the post-transplantation implantation miscarriage prevention treatment traditional Chinese medicine composition is used for achieving the effects of strengthening heart, assisting embryo, nourishing blood, preventing miscarriage and supporting early pregnancy; the three groups of traditional Chinese medicine components are subjected to progressive three-stage treatment to realize systematic intervention from eliminating evil to strengthening the body resistance and then to miscarriage prevention, so that the embryo implantation rate and the clinical pregnancy rate of a patient with repeated implantation failure are improved.

The present application relates to a kind of acute leukemia (ALL) high flux flow detection kit, belong to leukemia detection field, the present application is based on the original 8 color flow to increase 16 kinds of ALL cell antigen, can realize the simultaneous detection of 24 kinds of antigens in the same cell, not only greatly improve the detection precision of micro residual lesion (MRD), and by being divided into 12 development stages to ALL cell, can more accurately identify immunotyping when first diagnosis, and immunophenotypic aberration after bone marrow transplantation and after CAR-T cell therapy, this has very important guiding significance to the evaluation of patient treatment effect and the adjustment of treatment scheme.

The present invention relates to nano-graphene oxide having therapeutic effects on post-transplant complications such as graft-versus-host disease, nano-graphene oxide surface-modified with a zwitterionic polymer, macrophages treated with the nano-graphene oxide, and applications thereof. It was confirmed that administration of nano graphene oxide (NGO), NGO modified with a zwitterionic polymer, or macrophages treated with the NGO improves survival rates and effectively reduces tissue damage and inflammation in an animal model of acute graft-versus-host disease (GVHD), which occurs after transplantation. NGO regulates the activity and differentiation direction of immune cells, particularly macrophages, thereby reducing inflammation-induced (M1 type) macrophages, increasing the induction of immunomodulatory (M2 type) macrophages and regulatory T cells (Treg), and providing an immune homeostasis recovery effect even in patient-derived immune cells. Therefore, the present invention can be effectively utilized for treatment of immune diseases caused by post-transplant complications such as graft-versus-host disease.

Disclosed are methods for evaluating genetic models that are predictive of autoimmune disease phenotype or status. The methods comprise obtaining genotype data from bone marrow transplant recipients and donors. The autoimmune disease phenotype of the transplant recipient after the transplantation may be used evaluate the effect of genotype on the autoimmune disease. Phenotype comparisons may be made to the transplant donor and / or the transplant recipient prior to the transplantation. The methods may be used to validate genetic models associated with autoimmune disease. The genetic models may be based on one or more genetic variants associated with susceptibility to or protection from an autoimmune disease.

A kind of cell composition, including the cell cluster consisting of adipose-derived stem cells, endothelial cells and fibroblasts, as fat tissue regeneration unit.It is proved that the cell cluster composition of the application not only promotes the generation of fat, but also makes the generation of fat and angiogenesis and collagen formation etc.parallel, effectively promote the ability of fat tissue regeneration, while solving the problem of survival rate and insufficient vascularization after ADSCs transplantation.The combination of fat tissue regeneration unit and pGelMA not only solves the problem of vascularized fat tissue regeneration, but also promotes the formation of tissue similar to natural mammary tissue, providing a solution for realizing clinical breast reconstruction.

The invention relates to a long-acting hydrogen release system for assisting cell transplantation as well as a preparation method and application thereof, belongs to the technical field of medical treatment, and provides a long-acting hydrogen release system for assisting cell transplantation in order to provide a substance capable of performing targeted delivery to a cell transplantation part and continuously releasing high-dose H2. The long-acting hydrogen release system comprises a hydrogen release material with a particle size of 5 nm to 10 [mu] m and a hydrogel matrix, and the cells are dispersed in the system; the concentration of the hydrogen release material is 0.01 to 100 [mu] g / mL, and the content of the cells is 10000 to 100000000 cells / mL. The long-acting hydrogen release system is used for maintaining the activity and directional differentiation of transplanted cells in an in-vitro differentiation / balling process and maintaining the activity and characteristics of the transplanted cells in a pathological microenvironment after transplantation. In a malignant pathological microenvironment, hydrogen is continuously supplied, so that the activity of stem cell spheres assembled / differentiated into cartilage cell microspheres and the hyaline cartilage phenotype are effectively maintained, and the activity of transplanted stem cells and the hyaline cartilage differentiation potential are maintained by continuously supplying hydrogen; and continuously supplying hydrogen to maintain the activity and transparency of the transplanted cartilage cells.

The invention relates to a device for the transplantation of a Descemet's membrane-endothelium graft (5), comprising a longitudinal tube (1), the tube (1) having an inner cavity (2), an inlet opening (3) at its proximal end (PE) through which it is possible to introduce the graft (5) into the cavity (2), and an outlet opening (4) at its distal end (DE) through which it is possible to eject the graft (5) from the cavity (2), particularly into the anterior eye chamber of a patient, a guiding element (6) situated in the cavity (2) of the tube (1) extending from the proximal end area of the tube (1) or device towards the distal end area of the tube (1) or device and having an increasing cross section at least along a part of its extension, particularly by increasing the width of the guiding element (6) more than the height of the guiding element (6), wherein the guiding element (6) extending beyond the outlet opening (4) of the tube into the exterior environment of the tube (1), and having the distal end (6b) of the guiding element (6) being positioned outside of the tube (1) in front of the outlet opening (4) of the tube (1).

Methods and kits for screening, diagnosing, detecting or predicting a patient outcome / risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of one or more biomarkers selected from IL-6, IL-8, sTNFR1 and sTREM-1 in EVLP perfusate are described. The methods involve for example,i. obtaining one or more test EVLP perfusate samples of a donor lung;ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of one or more biomarkers selected from IL-8, IL-6, sTNFR1 and sTREM-1; andiii. a) comparing the polypeptide level of the one or more biomarkers in the perfusate sample with a control or cut-off level, wherein the differential level is indicative of outcome / risk of after transplant or of an EVLP outcome; orb) using the polypeptide level of one or several of the one or more biomarkers in combination, as part of an algebraic calculation of outcome / risk.

Provided is an intervertebral disc therapeutic agent that exhibits favorable post-transplantation engraftment. The intervertebral disc therapeutic agent contains a scaffold-free artificial tissue in which synovium-derived mesenchymal stem cells have formed a three-dimensional structure.

The invention belongs to the technical field of cell culture, and particularly relates to a culture medium for culturing regulatory T cells and a method for amplifying the regulatory T cells. The culture medium is composed of a T cell serum-free culture medium, IL-2, TGF-beta and rapamycin; 300 U to 600 U of IL-2, 5 ng to 10 ng of TGF-beta and 80 nmol to 120 nmol of rapamycin are added into each milliliter of the T cell serum-free culture medium. By improving the culture medium mother liquor and cell factor components of the Treg, the Treg with higher purity and more stable inhibition capability and phenotype is obtained, and a technical support is provided for maintaining immune tolerance by adopting a Treg cell treatment means after transplantation.

The application discloses a heterocyclic derivative with a structure shown in formula (I) or a stereoisomer, a pharmaceutically acceptable salt thereof. The heterocyclic derivative or the stereoisomer has high activity in inhibiting HCMV, and the activity is increased by 8-40 times compared with that of letermovir, and the patients with HCMV virus infection after allogeneic hematopoietic stem cell transplantation, kidney transplantation and lung transplantation can be better protected in clinic.

The present invention relates to a method for isolating dopaminergic neurons and to a pharmaceutical composition for the treatment of Parkinson's disease containing dopaminergic neurons isolated by said method. The method for isolating dopaminergic neurons comprises a step for isolating dopaminergic neurons that are positive for trophoblastic glycoprotein (TPBG), such that the dopaminergic neurons isolated by this method exhibit greater cellular efficacy upon transplantation and greater transplant safety, thus enabling their effective use in cell transplantation for the treatment of Parkinson's disease.

The invention discloses a method for predicting the risk of new pneumonia after hematopoietic stem cell transplantation based on pharyngeal microecology-immunity combination, and the method comprises the steps: carrying out the risk assessment solving through a pre-trained explainable prediction model of new pneumonia according to sampling data, and obtaining the risk prediction of new pneumonia. The method comprises the following steps: performing two-stage'fixed feature locking 'anti-leakage framework processing on sample data, and locking a core feature list and a corresponding binomial interaction structure to obtain a fixed feature locking anti-leakage framework; and based on the training set, probability mean value fusion is carried out through a fixed feature locking anti-leakage framework and L1 / L2 hybrid integrated logistic regression, and new pneumonia risk prediction is obtained. The invention discloses a risk prediction method and system for new pneumonia after hematopoietic stem cell transplantation based on pharyngeal microecology-immunity combination, and a storage medium. Prediction data processing of new pneumonia after transplantation is realized, and a plurality of detection parameters are fused to output a prediction risk through a new pneumonia risk prediction model.