86 results about "Post transplant" patented technology

A transport container and methods for preserving an organ at a target temperature. The transport container can maintain the organ at a temperature of 6-8⁰C for improved results after transplantation. The transport container can store the organ at a target temperature of determined based on characteristics of the organ and the donor. A processor can determine the target temperature for preservation of the organ. The transport container can contain cooling media that is colder near the bottom of the transport container and warmer near the top of the transport container to counter uneven cooling caused by gravity.

A transport container and methods for preserving a heart at a hypothermic temperature. The transport container can maintain the heart at a temperature of 6-8° C. for improved results after transplantation. The preservation of the heart within this range can improve transplantation outcomes.

The invention discloses a large-scale nursery stock transplanting method and system. The method comprises the following steps: S1, acquiring environment state parameters of a target nursery stock and environment state parameters of a target transplanting area; s2, detecting the growth distribution of the root system of the target nursery stock by adopting a multi-modal root system detection technology, and constructing a three-dimensional model of the root system distribution of the nursery stock; s3, determining an optimal root ball size and a cutting path based on a detection result in the step S2 and the morphological structure data of the nursery stock obtained in the step S1; s4, according to the cutting path determined in the step S3, accurately positioning and cutting the root ball of the target nursery stock, and binding the cut root ball; s5, according to the terrain where the target nursery stock is located and the weight of the nursery stock, the bound root ball is hoisted to a transportation tool through automatic hoisting equipment; s6, the seedlings transported to the target transplanting area are planted in the preset planting holes, and the planted seedlings are preliminarily fixed; and S7, performing post-transplantation maintenance and monitoring on the planted nursery stock.

Described herein are methods for treating antibody mediated rejection of transplanted organs using inhibitors of IL-6. In one embodiment, the IL-6 inhibitor is Tocilizumab and is administered simultaneously or sequentially with intravenous immunoglobulin (IVIG).

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

An isolated cell having a central memory T-lymphocyte (Tcm) phenotype, the cell being tolerance-inducing cell and capable of homing to the lymph nodes following transplantation, the cell being transduced to express a cell surface receptor comprising a T cell receptor signaling module is described. Methods of generating same and using same are also described.

The invention discloses application of sorafenib in maintenance treatment of acute myelogenous leukemia after transplantation, and relates to the technical field of medicines, and the key points of the technical scheme are as follows: it is found for the first time that secretion and killing functions of NK cells can be enhanced by using sorafenib for maintenance treatment after transplantation; sorafenib enhances communication between macrophages and NK cells by promoting secretion of macrophage proinflammatory factors, so that the function of NK cells is enhanced. The invention clarifies that sorafenib enhances the GVL effect of NK cells in a low tumor load environment, discusses the molecular mechanism of sorafenib for enhancing the NK cell function by promoting macrophage metabolism reprogramming, and provides a new thought for maintaining precision and optimization of treatment after transplantation.

The invention relates to an acute leukemia (ALL) high-throughput flow cytometry kit, and belongs to the field of leukemia detection.16 ALL cell antigens are added on the basis of an original 8-color flow cytometry, 24 antigens in the same cell can be detected at the same time, the detection precision of minimal residual focus (MRD) is greatly improved, and the detection sensitivity is improved. The ALL cells are divided into 12 developmental stages, so that immunological typing during primary diagnosis and immunophenotype variation after bone marrow transplantation and CAR-T cell treatment can be more accurately recognized, and the method has very important guiding significance on evaluation of treatment effects of patients and adjustment of treatment schemes.

The invention belongs to the technical field of medical equipment, and particularly discloses a reversible electroporation combined medical kit for organ transplantation and transfer and application of the reversible electroporation combined medical kit. According to the medical kit for organ transplantation, a novel electric pulse device is additionally designed on the basis of original design, a large amount of emergency response generated after organ transplantation can be remarkably reduced, and the survival rate of organs is increased. The novel electric pulse device is designed to be attached to the inner wall of the box body, the designed electric pulse electrode plate is attached to the buffer pad, the buffer pad is made of an insulating non-toxic heat preservation material, and organ preservation liquid is contained in the organ preservation bag. According to the medical kit for organ transplantation, the additional electrodes can be used for applying electric pulses to organs in the organ storage bag, medicine in the organ storage liquid is promoted to enter the organs, meanwhile, the optimal applied pulse intensity is confirmed in combination with cell experiments, the potential distribution condition is simulated and confirmed in combination with simulation software, and emergency reaction generated after perfusion can be reduced; therefore, normal organ functions are ensured, and the success rate of transplantation is improved.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention discloses a mesenchymal stem cell preparation and application thereof, and belongs to the technical field of cell biology. The mesenchymal stem cell preparation is a mesenchymal stem cell pretreated by a combined drug of ziyuglycoside II and shikonin. Through drug pretreatment, the antioxidant capacity of the mesenchymal stem cells is synergistically enhanced, the survival rate of the cells after transplantation is enhanced, the treatment effect of the cells on the NAFLD hepatocytes is remarkably enhanced, and lipid accumulation in the hepatocytes is effectively reduced. Therefore, the invention provides a novel method for enhancing the treatment capacity of the mesenchymal stem cells NAFLD, the method is suitable for bone marrow and umbilical cord mesenchymal stem cells, and an efficient and reliable preparation and an optimization scheme are provided for clinical stem cell treatment of the NAFLD.

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The invention discloses a method for improving the normal fertilization rate of an in-vitro ICSI embryo of a dairy cow and reducing the abortion rate, which comprises the following steps: preparing an ICSI egg from X-sex controlled sperm and an oocyte which is cultured by a mature oocyte and is used as a donor by adopting a microinjection mode, the ICSI eggs are incubated in an oocyte maturation culture solution (OM) for 3-5 h and then transferred to ETH to be activated for 4-6 min so as to promote normal fertilization of the ICSI eggs, then embryo culture is conducted, and then sexual control embryos are obtained; the ETH refers to an oocyte maturation culture solution containing 6 to 8 percent of ethanol. The normal fertilization rate of the ICSI embryos produced by the method is remarkably improved, and the early abortion rate is remarkably reduced in inspection after transplantation.

Nanoparticles, methods, and kits are provided for supplying nutrients and other therapeutic agents to transplanted cells. Nutrient deprivation is a significant factor which contributes to poor outcome of many cell transplants because cells receive insufficient nutrients until they are able to establish a functional microcirculation to support their metabolic and physiological needs after transplantation. Nanoparticles are provided for use in supplying nutrients and other therapeutic agents to transplanted cells to improve cell survival. Such nanoparticles can be used to supply nutrients and other factors to transplanted cells until the transplanted cells are able to develop a new microcirculation.

The invention relates to the technical field of greening transplanting, and discloses a road greening disc transplanting and maintaining all-in-one machine based on a soil moisturizing structure, the road greening disc transplanting and maintaining all-in-one machine comprises a supporting frame, a mounting frame and a base plate, the mounting frame and the base plate are arranged on the supporting frame, a transplanting mechanism is arranged between the mounting frame and the base plate, an auxiliary assembly is arranged on the base plate, and the auxiliary assembly comprises a maintaining part and a moisturizing part. In the working process, maintenance and moisture preservation work of transplanted green plants is achieved. The road greening disc transplanting and maintaining all-in-one machine based on the soil moisturizing structure can effectively solve the problems that in the prior art, when facing a large-scale green belt reconstruction project, the whole process depends on manual digging and carrying, a large amount of manpower needs to be consumed, meanwhile, due to the fact that the efficiency is low, the whole transplanting period is remarkably prolonged, and some small green plants are difficult to transplant in the transplanting process. In the prior art, the root system is exposed in the air for a long time, and physiological damage is easily caused by excessive water loss, so that the transplanted green plant is directly poor in growth.

This invention discloses a co-culture immune isolation device after cell transplantation, belonging to the technical field of cell culture devices. The device includes a culture plate with several first cavities inside and placement slots outside the first cavities. A cover plate is provided above the culture plate, and several second cavities are provided on the cover plate. A sealing ring is provided on the outside of the second cavities, and a liquid inlet mechanism is provided inside the cover plate, connected to the second cavities. This invention uses the above-mentioned co-culture immune isolation device after cell transplantation to achieve co-culture of multiple cells by setting multiple independent first cavities, preventing cell migration or direct contact and avoiding immune rejection. The protective cover and liquid inlet mechanism achieve closed-loop automatic liquid inlet, continuously diffusing signal molecules into the first cavities to maintain co-culture homeostasis and achieve co-culture of multiple cells.

The present invention provides a cell composition for treating detrusor underactivity and a method for preparing the same. The cell composition comprises a cell spheroid formed by seeding a plurality of mesenchymal stem cells in a culture device comprising a plurality of culture wells, wherein each of the culture wells has a surface coated with a low-protein-adhesion layer, and culturing the cells under the conditions to induce spheroid formation. Through the cell composition of the present invention, three-dimensional structured cell spheroids are formed to promote intercellular interactions and physiological function expression, thereby enhancing cell survival rates and functional performance after transplantation. In particular, the cell composition effectively facilitates the repair and restoration of contractile function of bladder muscle tissue, thereby improving the clinical symptoms associated with detrusor underactivity.

The invention belongs to the field of biological medicine, and particularly discloses a culture method of domesticated mesenchymal stem cells (MSCs), which comprises the following steps: separating plasma and platelet lysis buffer from autologous peripheral blood, and obtaining CTL and Treg through magnetic bead sorting; the method comprises the following steps: sequentially co-culturing umbilical cord MSCs for three generations: co-culturing the P1 generation and CTL in a culture medium containing 30% of alpha MEM, 68% of DMEM / F12, 1% of autologous plasma and 1% of platelet lysis buffer, and adding 0.1-1 [mu] M of resveratrol and 10-50 [mu] M of spermidine; co-culturing P2 and Treg in a culture medium containing 50% of alpha MEM, 48% of DMEM / F12, 1% of autologous plasma and 1% of platelet lysis buffer; the P3 generation is cultured and harvested in 90% of alpha MEM, 5% of DMEM / F12 and 5% of PRP. The obtained MSCs surface marker is CD105 < + > / CD90 < + > / CD73 < + > / CD45 <-> / CD34 <-> / HLA-DR <->, the expression quantity of CD24 is low (CD24low), the expression quantity of CD47 is high (CD47high), the expression quantity of PD-L1 is high (PD-L1 high), the IDO activity is larger than or equal to 25 U / mg, the retention rate of the lung after vein transplantation is smaller than or equal to 12%, and the survival period is larger than or equal to 28 days. The cell is suitable for treating ARDS or hepatic fibrosis.

The present disclosure is generally directed to the use of biomaterial scaffolds engineered with SA-FasL for the transplantation of stem cell derived β-cells as a treatment for Type I diabetes. Early engraftment post-transplantation and subsequent maturation of these β-cells may be limited by the initial inflammatory response, which impacts the ability to sustain normoglycemia at long times. The survival and development of immature hPSC-derived β-cells transplanted on poly(lactide-co-glycolide) (PLG) microporous scaffolds into the peritoneal fat, a site being considered for clinical translation, was investigated. The scaffolds were modified with biotin for binding of a streptavidin-FasL (SAFasL) chimeric protein to modulate the local inflammatory microenvironment. The presence of FasL impacted infiltration of monocytes and neutrophils and altered their phenotypic response. Conditioned media generated from scaffolds explanted at day 4 did not impact hPSC-derived β-cell survival and maturation in vitro, which was not observed with unmodified scaffolds. Following transplantation, β-cell viability and differentiation were improved with SA-FasL modification. A sustained increase in insulin positive cell ratio was observed with SA-FasL modified relative to unmodified scaffolds. These results demonstrate that SA-FasL-modified scaffolds can mitigate initial inflammatory response and enhance β-cell engraftment and differentiation.

The present application relates to the use of ZNF683 as a target in the diagnosis or therapeutic prognosis monitoring, and treatment of diseases related to the regulation of T cell immune tolerance. In particular, the present application studies the regulatory effect of ZNF683 on human primary T cells, and tests the feasibility of ZNF683 as a target for the prognosis and treatment of GVHD after clinical transplantation.

A method of producing oligodendrocytes recapitulates the major steps of oligodendrocyte differentiation in a significantly shorter time than the 75-95 days required for the previous protocol. The cannabinoid receptor agonist, WIN55212-2, induces NSC differentiation and promotes OLIG2+OPC induction. WIN-derived OLIG2+ progenitor cells differentiate into PDGFR α+ OPCs, which are highly migratory, can further differentiate into mature OLs with strong myelination ability. When WIN-derived OPCs were transplanted to the lesion site, the motor ability of spinal cord injury (SCI) mice was significantly improved from the second week after transplantation. Immunostaining results showed that WIN-derived OPCs could differentiate into mature OLs and myelinate injured axons. The method and product have wide applications in the field of cellular therapy, especially for spinal cord injury with remarkable therapeutic effects.

The present invention relates to the field of immunotherapy, in particular to diseases associated with Epstein-Barr virus (EBV, also known as human gamma herpesvirus 4), such as cancer or post-transplant lymphoproliferative disease, in particular to adoptive T cell therapy or T cell receptor (TCR) gene therapy. The present invention provides a combination of nucleic acids encoding at least two TCR constructs or corresponding proteins or host cells, wherein each TCR construct is capable of specifically binding to its respective epitope in the context of a respective MHC I, and wherein the epitopes are derived from different antigens expressed by the same infectious agent or cancer, such as EBV antigens. The present invention further provides specific nucleic acids encoding a TCR alpha chain construct (TRA) and / or a TCR beta chain construct (TRB) of a TCR construct specific for an epitope complexed with human MHC I, wherein the epitope is an epitope of an Epstein-Barr virus protein, the TCR construct being specific for an epitope from LMP2A, LMP1 or EBNA3C. Proteins encoded by said nucleic acids, corresponding host cells, as well as pharmaceutical compositions and kits are also an object of the present invention.

The invention belongs to the technical field of biological medicine, and particularly relates to composite hydrogel for promoting hiPSC-CMs in-situ maturation and a preparation method and application thereof. According to the preparation method disclosed by the invention, a compound liposome modified by quaternary ammonium chitosan and loaded with Torrin 1 is constructed, a unique three-dimensional network structure (PDN) is constructed by compounding different chitosan, and the compound liposome and the PDN are combined to obtain the composite hydrogel for embedding and delivering hiPSC-CMs. Wherein residual amine of the quaternary ammonium chitosan can slowly release drugs in an acidic microenvironment after myocardial infarction, and the cell cycle is inhibited; the PDN structure enhances the retention rate of cell transplantation; the loaded hiPSC-CMs can restore the contraction function, reduce spontaneous depolarization and action potential, reduce the risk of lethal arrhythmia and promote in-situ maturation of transplanted cells. Experiments show that the gel loaded with the hiPSC-CMs can significantly promote myocardial tissue maturation after myocardial infarction, effectively slow down ventricular remodeling and improve heart functions.

The present invention provides compositions and methods for promoting the engraftment of exogenous hematopoietic stem cell grafts and for maintaining the pluripotency and hematopoietic function of these cells after transplantation. [Solution] The present invention provides compositions and methods useful for the depletion of cells such as CD45+, CD135+, CD34+, CD90+, and / or CD110+ cells, and, in particular, for the treatment of various hematopoietic disorders, metabolic disorders, cancer, and autoimmune diseases. For example, antibodies, their antigen-binding fragments, ligands, and conjugates that can be applied to achieve the treatment of these conditions by depleting populations of D45+, CD135+, CD34+, CD90+, or CD110+ cells in a patient such as a human are described herein.

The present disclosure provides engineered T cells with reduced expression or knock out of an immunophilin, and which express a chimeric antigen receptor (CAR) and / or comprise one or more modifications that disrupt MHC class I and / or MHC class II alleles and increase expression of a tolerogenic factor. The present disclosure also provides methods of treating a subject having or suspected of having a post-transplant disorder by administering T cells with reduced expression or knock out of an immunophilin. Also disclosed are pharmaceutical compositions comprising T cells with reduced expression of an immunophilin, for use in treating a subject having or suspected of having a post-transplant disorder.

The invention belongs to the field of bioelectromagnetism, and particularly discloses a method for improving functions of frozen and thawed ovarian tissues by adopting an mT-level pulsed magnetic field. According to the method, ovarian tissue which is subjected to vitrification freezing and resuscitation is exposed in a pulsed magnetic field with specific parameters for intervention treatment; wherein the intensity of the pulsed magnetic field is 5 mT, the current period is 1000-6000 [mu] s, the duty ratio is 50%, the pulse repetition number is 30, and the pulse interval is 60 ms. According to the application, a non-intrusive and drug-residue-free physical intervention means is adopted, inflammatory response can be effectively inhibited, oxidative stress injury can be relieved, the number of functional mitochondria is increased by up-regulating mitochondrial fusion genes (such as Mfn1 and Mfn2), the energy metabolism state of cells is fundamentally improved, the survival rate of transplanted ovarian tissue is remarkably increased, the functional life of the ovarian tissue is prolonged, and the survival rate of the ovarian tissue is increased. The method is simple to operate, low in cost and good in clinical application prospect.

The invention discloses a multi-phase CT image-based early recurrence risk prediction method and system after hepatocellular carcinoma liver transplantation, and belongs to the technical field of medical image processing and artificial intelligence. The method comprises the following steps: S1, multi-phase CT image acquisition and standardization preprocessing; s2, sketching a tumor area and a peritumor area to form a target volume; s3, extracting radiomics features and deep learning features; s4, performing multiple rounds of feature screening, and combining clinical risk factors to construct a deep learning radiomics column graph prediction model; s5, risk prediction and layering; and S6, combining with the traditional clinical standard to realize increment value integration. The system comprises an image access and preprocessing module, a feature calculation engine, a model management module, a risk assessment module and a clinical decision support module. The problems of insufficient information utilization, difficulty in clinical integration and the like in the prior art are solved by jointly analyzing the tumor and the peritumoral region and fusing multi-dimensional features, the early recurrence prediction precision is improved, and support is provided for clinical decision making.

The present invention relates to glycyrrhizin-glycol chitosan conjugate-coated nanoparticles, islet cells, prepared using same, for transplantation, and an MRI imaging composition comprising same. If transplanted, the islet cells comprising the nanoparticles can suppress a post-transplantation immune response. The present invention can provide islet cells for transplantation that can be transplanted to a certain region by magnetic force induction and can be tracked by MRI.

The invention relates to the technical field of transplantation medicine and pharmacology crossing, in particular to application of Xuebijing injection in preparation of a medicine for inhibiting instant blood-mediated inflammatory response after portal vein pancreas islet transplantation. According to the application, the application is innovatively applied to prevention and treatment of instant blood-mediated inflammatory response in pancreas islet transplantation, complement activation, blood coagulation cascade and inflammatory cell infiltration are synergistically inhibited through multiple targets, and the curative effect bottleneck of a traditional single anti-coagulation or anti-inflammatory strategy is broken through; the invention provides a Chinese and western medicine combined patent solution which has the advantages of efficient regulation and safety for overcoming early-stage key inflammatory injury after transplantation.

The invention features the use of coelenterazine or similar analogs as a real-time reporter for systemic administration of ROS or RNS levels in the animal cell and organ environment. This real-time measurement can then be used to assess the severity of free radical dependent disease states, such as neurodegenerative disease, ischemia-reperfusion injury after organ transplantation, acute radiation syndrome injury, and the like. Methods may include real-time measurement of therapeutic efficacy of PrC-210 as a radical scavenger capable of eliminating oxygen and nitrogen radicals.