239 results about "Post transplant" patented technology

A transport container and methods for preserving an organ at a target temperature. The transport container can maintain the organ at a temperature of 6-8⁰C for improved results after transplantation. The transport container can store the organ at a target temperature of determined based on characteristics of the organ and the donor. A processor can determine the target temperature for preservation of the organ. The transport container can contain cooling media that is colder near the bottom of the transport container and warmer near the top of the transport container to counter uneven cooling caused by gravity.

A transport container and methods for preserving a heart at a hypothermic temperature. The transport container can maintain the heart at a temperature of 6-8° C. for improved results after transplantation. The preservation of the heart within this range can improve transplantation outcomes.

Described herein are prediction models based on the transcriptomic, exomic, and / or radiological analyses on tissue samples to predict the likelihood of the original cancer (such as Hepatocellular carcinoma (HCC)) recurrence into the liver transplant. An example computer implemented method for predicting the likelihood of liver cancer recurrence 5 into a liver transplant includes receiving gene expression data related to a liver tissue sample for a subject having a liver cancer, inputting the gene expression data into a trained machine learning model, and predicting, using the trained machine learning model, a risk of recurrence of the liver cancer in the subject after liver transplantation.

The art does not provide systematic and reproducible methods to personalize dosing of multiple immunosuppressive medications after transplantation. This invention provides a method to systematize multi-drug immuno suppression management in tissue and organ transplantation using an artificial intelligence-based complex systems approach. In embodiments of this invention, immunosuppression drug dose, blood drug concentrations, donor-derived fraction of cell free DNA (dd-cfDNA %), and aspartate aminotransferase are used to indicate allograft status or a proxy for allograft status, to generate a phenotypic response surface to produce individual treatment modalities and dosages using empirically determined unique coefficients. This surface is used to calculate appropriate immunosuppression drug doses associated with the desired outcome for that patient. Embodiments of this disclosure are directed to identifying optimized combinations of inputs for the complex system of the immunosuppressed transplant patient in order to avoid transplant rejection while avoiding unnecessary toxicity and maintaining a robust enough immune response to fight infection.

The invention discloses a large-scale nursery stock transplanting method and system. The method comprises the following steps: S1, acquiring environment state parameters of a target nursery stock and environment state parameters of a target transplanting area; s2, detecting the growth distribution of the root system of the target nursery stock by adopting a multi-modal root system detection technology, and constructing a three-dimensional model of the root system distribution of the nursery stock; s3, determining an optimal root ball size and a cutting path based on a detection result in the step S2 and the morphological structure data of the nursery stock obtained in the step S1; s4, according to the cutting path determined in the step S3, accurately positioning and cutting the root ball of the target nursery stock, and binding the cut root ball; s5, according to the terrain where the target nursery stock is located and the weight of the nursery stock, the bound root ball is hoisted to a transportation tool through automatic hoisting equipment; s6, the seedlings transported to the target transplanting area are planted in the preset planting holes, and the planted seedlings are preliminarily fixed; and S7, performing post-transplantation maintenance and monitoring on the planted nursery stock.

The invention discloses a method for optimizing the maturation effect of cattle and sheep oocytes through CNP pretreatment, and relates to the technical field of biological breeding, the method comprises the following steps: S1, selecting and treating donor cells; s2, collection of oocytes; s3, in-vitro maturation culture of the oocytes: after the pre-maturation treatment, transferring the oocytes into a maturation culture solution for continuous culture under the in-vitro culture condition of air containing 5% CO2, keeping the culture temperature of the oocytes within 1-20 hours at 38.5-39 DEG C, and keeping the culture temperature of the oocytes within 20-28 hours at 39-39.5 DEG C; s4, culturing and screening reconstructed embryos; s5, preparing and transplanting a receptor animal; and S6, performing subsequent monitoring and management. According to the method, the culture temperature is kept at 38.5-39 DEG C within 1-20 hours, the culture temperature is kept at 39-39.5 DEG C within 20-28 hours, a series of enzymes and signal channels in the oocytes can be effectively activated by accurately controlling the culture temperature, and the survival rate and the development quality of the oocytes are improved.

A method of generating an isolated population of non graft versus host disease (GvHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and / or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) providing a population of at least 70% memory T cells; (b) contacting the population of memory T cells with an antigen or antigens so as to allow enrichment of antigen reactive cells; and (c) culturing the cells resulting from step (b) in the presence of cytokines so as to allow proliferation of cells comprising the Tcm phenotype. Cells generated by the method, pharmaceutical compositions and methods of treatment are also disclosed.

Described herein are methods for treating antibody mediated rejection of transplanted organs using inhibitors of IL-6. In one embodiment, the IL-6 inhibitor is Tocilizumab and is administered simultaneously or sequentially with intravenous immunoglobulin (IVIG).

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

An isolated cell having a central memory T-lymphocyte (Tcm) phenotype, the cell being tolerance-inducing cell and capable of homing to the lymph nodes following transplantation, the cell being transduced to express a cell surface receptor comprising a T cell receptor signaling module is described. Methods of generating same and using same are also described.

The invention relates to the technical field of cartilage construction, in particular to an injectable tissue engineering cartilage in-vitro construction method which comprises the following steps: S1, micro-ecological cartilage environment simulation design; s2, self-assembly of the stent and space-time guidance; and S3, constructing a reversible tissue-degradation platform. A tissue engineering system highly simulating a natural cartilage microenvironment is constructed through the technology of combining bionic design and dynamic regulation and control, the proliferation activity and functional differentiation efficiency of cartilage cells can be effectively improved, and meanwhile, by means of intelligent support design and a growth factor delivery system, the tissue engineering system can be applied to the field of cartilage tissue engineering. Accurate release of factors and cell navigation are realized; the dynamic repair technology is combined with the non-contact mechanical stimulation and immune regulation strategy, it is ensured that transplanted tissue can be continuously generated and fully integrated with host tissue, the problems of immunological rejection or insufficient stent degradation and the like are solved, the success rate and long-term stability of cartilage regeneration are remarkably improved, and the method has good clinical application prospects.

The invention discloses a gene combination for risk prediction of acute graft versus host disease and donor screening before hematopoietic cell transplantation and application of the gene combination. According to the present invention, the gene combination with the post-transplantation aGVHD occurrence prediction and diagnosis value is found through the whole genome sequencing and the target gene capture sequencing method; on one hand, comprehensive judgment and evaluation are carried out by detecting gene combination mutation conditions of donors and recipients, selection of transplantation donors is optimized, and the survival rate and the survival quality of patients are improved; on the other hand, the occurrence of aGVHD after transplantation of the recipient can be predicted by detecting the gene combination mutation condition of the donor and the recipient, and a basis is provided for potential aGVHD diagnosis; and thirdly, a new genetic basis is provided for revealing the pathogenesis of the aGVHD, and a new drug target is provided for treating the aGVHD.

The invention relates to the field of medical examination, in particular to application of a substance for detecting an SSBP2-FLT3 fusion gene in preparation of a product for detecting human leukemia. Aiming at the fusion gene SSBP2-FLT3, a specific PCR primer is designed, PCR amplification and sequencing are performed after nucleic acid is extracted from a patient sample, and the specific translocation site of the fusion gene is detected, so that the method is the most accurate, convenient and economical method. According to the application, DNA fusion gene detection and RNA fusion gene detection are combined, and meanwhile, as a molecular marker, an important basis can be provided for selecting a treatment scheme for a patient, predicting a recurrence risk and evaluating prognosis, and the molecular marker has an irreplaceable important effect in aspects of hematopoietic stem cell transplantation curative effect evaluation, pretreatment scheme optimization and post-transplantation molecular recurrence monitoring.

The invention discloses application of sorafenib in maintenance treatment of acute myelogenous leukemia after transplantation, and relates to the technical field of medicines, and the key points of the technical scheme are as follows: it is found for the first time that secretion and killing functions of NK cells can be enhanced by using sorafenib for maintenance treatment after transplantation; sorafenib enhances communication between macrophages and NK cells by promoting secretion of macrophage proinflammatory factors, so that the function of NK cells is enhanced. The invention clarifies that sorafenib enhances the GVL effect of NK cells in a low tumor load environment, discusses the molecular mechanism of sorafenib for enhancing the NK cell function by promoting macrophage metabolism reprogramming, and provides a new thought for maintaining precision and optimization of treatment after transplantation.

Disclosed are novel strategies for the treatment of patients with Parkinson's disease and other secondary Parkinsonian disorders. Disclosed are DA neuronal cells that have been modified in vitro with a genetic insertion of GDNF. The GDNF coding sequence is inserted under the transcriptional control of a promotor such that secreted proteins are made and taken up by the endogenous cells after the administered engineered cells have matured to a Neuronal Mature Cell Type to promote survival of endogenous neurons. Also disclosed are DA neuronal cells that have been modified with a genetic insertion of GBA in vitro and are hemizygous null for SNCA. The GBA coding sequence is inserted under the transcriptional control of a ubiquitous promotor and the secreted proteins are made and taken up by the graft and endogenous cells immediately upon transplantation to promote long-term graft integrity.

The utility model provides a human intestinal flora transplantation device, which belongs to the technical field of flora transplantation and comprises a sterilization cylinder and a transplantation component arranged on the inner wall of the sterilization cylinder, the transplantation component comprises a sealing cylinder, a transplantation port, a rubber sleeve, a blocking ring, degreasing cotton, a transplantation pipe, a supply pipe, a conveying pipe and a lifting component, the sealing cylinder is embedded in the inner wall of the sterilization cylinder, and the rubber sleeve is embedded in the sealing cylinder. The transplanting opening is formed in the center of the outer wall of one end of the sealing cylinder, the blocking ring is embedded in the inner wall of the sealing cylinder, the rubber sleeve is embedded in the inner wall of one end of the sealing cylinder, and the degreasing cotton is embedded in the inner wall of one end of the sealing cylinder. By means of the transplanting assembly, after transplanting is completed, efficient sterile storage can be conducted on the transplanting pipe, breeding of microorganisms is avoided, meanwhile, multiple sterilization modes are adopted, continuous sterilization is conducted on the transplanting pipe, infectious microbes are prevented from entering an in-vivo digestive system, the transplanting quality is improved, and the situation that the transplanting pipe is polluted by the external environment within the waiting time of disposable pipe implantation is avoided; meanwhile, dust attachment is avoided.

The invention relates to an acute leukemia (ALL) high-throughput flow cytometry kit, and belongs to the field of leukemia detection.16 ALL cell antigens are added on the basis of an original 8-color flow cytometry, 24 antigens in the same cell can be detected at the same time, the detection precision of minimal residual focus (MRD) is greatly improved, and the detection sensitivity is improved. The ALL cells are divided into 12 developmental stages, so that immunological typing during primary diagnosis and immunophenotype variation after bone marrow transplantation and CAR-T cell treatment can be more accurately recognized, and the method has very important guiding significance on evaluation of treatment effects of patients and adjustment of treatment schemes.

The present invention relates to a stem cell membrane-forming culture medium and its application. The stem cell membrane-forming culture medium includes a cell basal culture medium, fetal bovine serum, growth factors, an attachment skeleton material and vitamins, and relates to the preparation of a stem cell membrane-forming culture medium, the separation of stem cells, the culture of stem cells and the formation of stem cell membranes. The beneficial effects of the present invention are as follows: the membrane-forming method of the present invention utilizes the solubility property of collagen itself to form a membrane by low-temperature centrifugation, which has low operating costs and technical requirements and is conducive to large-scale production; the stem cell biological patch finally obtained by the present invention has high immunocompatibility, few adverse reactions, thin membrane thickness and controllable thickness according to temperature and centrifugation time, and because the present invention embeds stem cells into the biological patch membrane, the stem cells are slowly released as the matrix is ​​absorbed by the body after transplantation, so the onset time is longer, and the effect of repairing the ovary is better than that of the stem cell biological patch prepared by ordinary culture adsorption.

A method for handling, treating, and storing a cornea is disclosed that enables vitrification and rewarming in air and / or liquid nitrogen vapor without causing significant cell loss, cell death, or dysfunction after transplantation, as compared to untreated control corneas. The method utilizes osmotic imbalance and extreme corneal shrinkage due to corneal dehydration, and enables avoidance of cryoprotectant toxicity, even when exposed to the peak (vitrifiable) concentration of cryoprotectant above 0 °C for an extended period of time or when washing the cryoprotectant without performing osmotic buffering. The corneal device enables ideal handling and storage and air cooling inside or outside the container used for storage.

The invention belongs to the technical field of medical equipment, and particularly discloses a reversible electroporation combined medical kit for organ transplantation and transfer and application of the reversible electroporation combined medical kit. According to the medical kit for organ transplantation, a novel electric pulse device is additionally designed on the basis of original design, a large amount of emergency response generated after organ transplantation can be remarkably reduced, and the survival rate of organs is increased. The novel electric pulse device is designed to be attached to the inner wall of the box body, the designed electric pulse electrode plate is attached to the buffer pad, the buffer pad is made of an insulating non-toxic heat preservation material, and organ preservation liquid is contained in the organ preservation bag. According to the medical kit for organ transplantation, the additional electrodes can be used for applying electric pulses to organs in the organ storage bag, medicine in the organ storage liquid is promoted to enter the organs, meanwhile, the optimal applied pulse intensity is confirmed in combination with cell experiments, the potential distribution condition is simulated and confirmed in combination with simulation software, and emergency reaction generated after perfusion can be reduced; therefore, normal organ functions are ensured, and the success rate of transplantation is improved.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention relates to an isolated culture method of adipose-derived stem cells. The isolated culture method comprises the following steps: S1, aseptically acquiring adipose tissues of a human body; s2, cutting the obtained adipose tissue into small blocks of 1-2mm < 3 >, putting the small blocks into a sterile culture bottle for culture, and digesting to obtain a uniform suspension; s3, filtering the digested suspension through a cell filter, carrying out centrifugal treatment on the filtered liquid, and removing supernate to obtain cell precipitate; s4, after the cell precipitates are resuspended, a culture dish is inoculated with the cell precipitates, the cell precipitates are put into an incubator to be cultured, a culture medium is replaced regularly, the culture medium contains acetic acid, and after passage operation, adipose-derived stem cells obtained after passage culture are obtained; and S5, detecting and cryopreserving. According to the application, the stress resistance of the adipose-derived stem cells is improved through pretreatment by utilizing the protection effect of acetic acid under a cell stress condition, and the adaptability of the adipose-derived stem cells is improved by simulating an in-vivo survival microenvironment through a pretreatment strategy, so that the survival rate of the adipose-derived stem cells in the survival microenvironment after transplantation is improved, and the survival rate of the adipose-derived stem cells is improved. The application potential in tissue repair and regeneration treatment is enhanced.

The embodiment of the invention provides a pancreas islet differentiation method and equipment for microencapsulated induced pluripotent stem cells, and relates to the field of stem cell differentiation. In order to solve the problems of low differentiation efficiency, low insulin secretion function and immunological rejection after transplantation of the two-dimensional cultured iPSC, a method for inducing the iPSC into the islet cells under a three-dimensional condition is constructed, the differentiation efficiency and the functions of the islet cells are improved, immune isolation transplantation can be directly performed after differentiation, and meanwhile, new equipment is constructed. The structure is beneficial to improving the mass transfer efficiency in the induction process, and large-scale and accurate condition control can be realized. The preparation method comprises the following steps: S1, mixing iPSC with a sodium alginate solution to form calcium alginate microspheres containing the iPSC; s2, culturing the iPSC in the calcium alginate microspheres obtained in the step S1 into a cell cluster; and S3, performing stage induced differentiation on the cell cluster obtained in the step S2 to obtain the endocrine cell cluster.

Provided are a detection reagent for acute graft-versus-host disease (aGVHD) and the use of the percentage of CD38+HLA−DR+CD8+ T cell as a biomarker for aGVHD. The present application has unexpectedly found that a proportion of the CD38+HLA−DR+CD8+ T cell in peripheral blood of a patient with aGVHD is significantly greater than that in peripheral blood of a healthy donor and a patient without aGVHD after transplantation. The receiver operating characteristic (ROC) curve analysis shows that the CD38+HLA−DR+CD8+ T cell has an area under the ROC curve (AUC) of 0.84, with a sensitivity of 80.8%, and a specificity of 75.0% when used in early diagnosis of aGVHD. Results indicate that the CD38+HLA−DR+CD8+ T cell is a marker for early diagnosis of aGVHD with a prominent diagnostic effect. The marker can guide the clinical medication.

The invention provides application of circRNA 3254 in preparation of a preparation or a medicine for preventing early apoptosis of transplanted islet cells, belongs to the technical field of islet transplantation, and aims to solve the important problems of relieving the early transplanted islet apoptosis, improving the transplanted islet function and prolonging the time of completely separating from insulin after transplantation in the current islet transplantation field. A circRNA sequencing result finds that circRNA participates in a process of improving the curative effect of transplanted pancreas islet by bone marrow mesenchymal stem cells (BMSCs) for the first time, and circRNA 3254 which is not reported in literatures is screened as a research target. The specific regulation effect of circRNA 3254 on the apoptosis condition of the early transplanted pancreas islet is verified in in-vivo and in-vitro models for the first time, and a mechanism network for improving the survival rate of the transplanted pancreas islet by the BMSCs is further improved. The specific regulation effect of circRNA 3254 on the apoptosis condition of the early transplanted pancreas islet is verified in in-vivo and in-vitro models for the first time, and a mechanism network for improving the survival rate of the transplanted pancreas islet by the BMSCs is further improved.

A three-dimensional oxygen-generating bioscaffold capable of supplying cells with a continuous, controlled, and steady source of oxygen and methods of using such an oxygengenerating bioscaffold to prevent hypoxia-induced damage to cells following transplantation are disclosed. In particular, a collagen-based cryogel bioscaffold having calcium peroxide (CPO) incorporated into its matrix is provided, wherein the CPO produces oxygen upon exposure to water. The collagen-based cryogel is designed with a plurality of macropores capable of encapsulating therapeutic cells for cellular therapy. Methods of producing oxygen-generating bioscaffolds and tissue grafts comprising therapeutic cells contained in such oxygen-generating bioscaffolds as well as their use in cellular therapy are also disclosed.

The invention discloses a mesenchymal stem cell preparation and application thereof, and belongs to the technical field of cell biology. The mesenchymal stem cell preparation is a mesenchymal stem cell pretreated by a combined drug of ziyuglycoside II and shikonin. Through drug pretreatment, the antioxidant capacity of the mesenchymal stem cells is synergistically enhanced, the survival rate of the cells after transplantation is enhanced, the treatment effect of the cells on the NAFLD hepatocytes is remarkably enhanced, and lipid accumulation in the hepatocytes is effectively reduced. Therefore, the invention provides a novel method for enhancing the treatment capacity of the mesenchymal stem cells NAFLD, the method is suitable for bone marrow and umbilical cord mesenchymal stem cells, and an efficient and reliable preparation and an optimization scheme are provided for clinical stem cell treatment of the NAFLD.

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The invention discloses injectable hierarchical structure composite hydrogel for pancreas islet transplantation and a preparation method of the injectable hierarchical structure composite hydrogel, and belongs to the technical field of biomedical engineering. The preparation method comprises the following steps: introducing bubbles into an inner phase tube in a microfluidic device, introducing a hydrogel cell pre-polymerization solution into an outer phase tube, uniformly distributing the bubbles in the hydrogel cell pre-polymerization solution, then mixing with an ionic crosslinking aqueous phase solution, irradiating with blue light to obtain bubble-loaded hydrogel microfibers, and obtaining a pancreas islet-loaded short fiber stent after the bubbles are broken; mixing the oxidized sodium alginate solution with the carboxymethyl chitosan solution, and wrapping the angiogenesis promoting component to obtain a self-healing hydrogel matrix; and mixing the short fiber scaffold with the self-healing hydrogel matrix to obtain the injectable hierarchical structure composite hydrogel. The composite hydrogel has good biocompatibility, oxidation resistance and immune isolation effect, meanwhile, the hierarchical structure promotes formation of external vascularization, and long-term survival and function maintenance of transplanted plants after pancreas islet transplantation are facilitated.

The invention relates to an exosome of an organoid-derived human retinal progenitor cell as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a retinal progenitor cell exosome separated from a retinal organ differentiated from human embryonic stem cells. The exosome has a specific marker. Through hESCs culture, nerve induction, organoid formation, cell sorting and exosome extraction, the exosome with high purity and high biological activity is successfully obtained, and the retinal structure and visual function of an animal model can be remarkably changed after the exosome is transplanted to the subretinal space. The exosome has a potential application value in preparation of a human retinal degenerative disease medicine preparation, and is expected to provide a new thought and method for treatment of retinal diseases.