12 results about "Mature oocyte" patented technology

The present invention belongs to the field of molecular biology and genetics, and in particular relates to a method for editing a bovine gene based on Pro-iCHI. The present invention provides a method for editing a bovine gene based on Pro-iCHI. Protamine is transiently expressed in gene-edited b-haSCs, which are then injected to mature oocytes to obtain reconstructed embryos. Protamine can eliminate abnormal DNA methylation resulting from oocyte intracytoplasmic haSCs injection and enable the nucli to compress into sperm-like structures, and the obtained bovine Pro-iCHI embryos can successfully develop into blastocysts, with a blastocyst rate comparable to that of the embryos obtained by in vitro fertilization. Moreover, in the present invention, a protamine-encoding gene is inserted into a Saccharomyces cerevisiae protein expression vector for transient expression, which ensures that abnormal DNA methylation is erased, without integration into the genome resulting in the insertion of exogenous genes.

The present invention relates to a culture medium composition for in vitro maturation of oocytes, comprising piperine as an active ingredient, and an in vitro embryo production method using same. The culture medium composition for in vitro maturation of oocytes, comprising piperine as an active ingredient, of the present invention, can qualitatively improve oocytes by inhibiting reactive oxygen species, and in vitro embryo production efficiency can be increased using in vitro matured oocytes obtained from the medium of the present invention.

The invention belongs to the technical field of embryo in-vitro culture, and discloses a method for in-vitro production of high-quality embryos by using frozen boar semen. In the in-vitro production process of the pig embryo, the fertilization rate and the quality of the in-vitro cultured embryo are improved by using the frozen semen treated by the antifreeze protein NT5C1B and the mature oocyte through in-vitro fertilization. Wherein the selected protein can reduce the generation of ice crystallization in the freezing process of the semen, improve the quality and fertilization ability of the frozen semen, and provide a basis for high-quality development of embryos after in-vitro fertilization. The application provided by the invention is simple and convenient to operate and remarkable in effect, and does not generate any harmful influence on animals. The invention provides a powerful theoretical basis for in-vitro production of high-quality embryos.

The application belongs to the technical field of pest control, and discloses application of a transcription factor CG17328 in control of drosophila melanogaster. The application discloses application of CG17328 in regulation of insect reproductive capacity. The application firstly screens a transcription factor for regulating the 'dumping' process of nurse cells in oogenesis of drosophila melanogaster by using transcriptome sequencing, genetics and molecular biology and other related technologies and means. The transcription factor is a ZAD-C2H2 type zinc finger protein CG17328. CG17328 is mainly located in the nuclei of nurse cells and oocytes in the late stage of oogenesis. CG17328 can affect the oogenesis process by regulating the 'dumping' process of nurse cells. The loss of CG17328 expression leads to significant decrease of the number of mature oocytes generated by ovaries and the hatching rate of eggs, which indicates that the loss or inhibition of CG17328 expression can achieve the control effect on drosophila melanogaster.

This invention provides a method to improve age-related decline in ovarian function by supplementing quiescent oocytes within primordial follicles. [Solution] The present invention provides a method for inducing mature oocytes, comprising the steps of: preparing a single-cell suspension from the ovary of a mammal other than a human; separating the single-cell suspension into oocytes and ovarian somatic cells (supporting cells) within a primordial follicle using a flow cytometer; re-aggregating the oocytes and ovarian somatic cells within the separated primordial follicle; and maturing the re-aggregated primordial follicle in vitro.

The invention discloses a culture solution for promoting in-vitro maturation of bovine oocytes and application of the culture solution, and belongs to the technical field of assisted reproduction. The invention provides the culture solution which is composed of brand new components and is used for promoting in-vitro maturation of the bovine oocytes, support is provided for maturation of the oocytes from multiple aspects of carrier transportation, energy metabolism regulation, signal transduction, extracellular environment and the like, the in-vitro maturation quality of the bovine oocytes can be effectively promoted, and the survival rate of the bovine oocytes is increased. The quality and consistency of nucleus maturation and cytoplasm maturation of oocytes are obviously improved; the cultured mature oocytes are used for in-vitro fertilization, so that the fertilization proportion and the blastocyst hatching rate can be remarkably improved; the provided culture solution is appropriate in component number, clear in proportion, simple and easy to operate in preparation method, short in time consumption, good in repeatability and suitable for batch production, and has a good application prospect in maturation culture of bovine in-vitro oocytes.

The application relates to the field of super-low-temperature cryopreservation of mammalian oocytes, and particularly relates to application of miR-32 in improving the development capacity of pig immature oocytes after freezing. Pig immature oocytes are frozen and cultured in vitro, and mature oocytes which are cultured to expel the first polar body are incubated in transfection liquid containing a miR-32 nucleotide sequence for 2-6 hours; the pig immature oocytes are GV stage oocytes; and the miR-32 nucleotide sequence is shown in SEQ ID NO:1. It is found that miR-32 can be used as a key marker molecule of damage of vitrification oocytes, and by transfecting miR-32 mimics into the frozen oocytes, the early embryo development capacity of the oocytes can be improved, a new solution is provided for improving the maturation quality of the frozen oocytes, and various embryo biological technology researches are facilitated.

A system for predicting the number of mature oocytes in a subject, comprising: a data acquisition module, used for obtaining data of the age, basal anti-Mullerian hormone (AMH) level, basal follicle-stimulating hormone (FSH) level or basal antral follicle count (AFC), and dynamic change of an inhibin B level in an early follicular phase (ΔINHB / difference between inhibin B on the sixth day and inhibin B on the 2nd day of menstruation in an ovulation induction cycle) of the subject; and a mature oocyte number calculation module, used for calculating the data obtained in the data acquisition module, so as to calculate the number of retrieved mature oocytes (NROs) in the subject. According to the system and method of the present application, the dynamic change of the inhibin B level in the early follicular phase is used as an evaluation index to replace an AFC index having a plurality of defects in the prior art, so that a better effect of predicting the NROs is achieved. The system can be used for predicting the NROs in an ovulation induction process (such as the sixth day of menstruation in the ovulation induction cycle) according to a dynamic change index, thereby adjusting the dosage of an ovulation induction drug.

A method of providing a culture of oogonia stem cells comprising oogonia stem cells is provided. The method may further include culturing the oogonia stem cells with granulosa and theca cells to differentiate the oogonia stem cells into oocytes. In some embodiments, the culturing comprises including the oogonia stem cells in an in vitro follicle construct or a microcapsule comprising said granulosa and theca cells. Further described herein is a method of forming a bioengineered follicle construct capable of releasing a mature oocyte. An in vitro fertilization method using the mature oocyte is also provided.

A method for inducing immature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells. A method for producing mature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells, and co-culturing the cell obtained after the introduction and ovarian somatic cells.

A method of providing a culture of oogonia stem cells comprising oogonia stem cells is provided. The method may further include culturing the oogonia stem cells with granulosa and theca cells to differentiate the oogonia stem cells into oocytes. In some embodiments, the culturing comprises including the oogonia stem cells in an in vitro follicle construct or a microcapsule comprising said granulosa and theca cells. Further described herein is a method of forming a bioengineered follicle construct capable of releasing a mature oocyte. An in vitro fertilization method using the mature oocyte is also provided.

The invention relates to the technical field of animal breeding biology, and particularly discloses a method for improving the development efficiency of in-vitro embryos of young cattle and sheep, which comprises the following steps: collecting oocytes from an in-vitro ovary, washing by using a conventional in-vitro maturation solution added with enoxone, and drying to obtain the in-vitro embryos of the young cattle and sheep. Carrying out pretreatment culture for 2-4 hours in a conventional in-vitro maturation culture solution added with enoxone, and then transferring into the conventional in-vitro maturation culture solution to continuously culture for 20-26 hours; and carrying out in-vitro fertilization on the mature oocytes to obtain fertilized eggs, and carrying out in-vitro culture for 8-10 days. According to the method provided by the invention, the cleavage rate and blastocyst development rate of oocytes of calves and lambs after in-vitro fertilization are improved by adding Enoxilone into an in-vitro maturation culture solution.