5 results about "Cumulus Cell" patented technology

The invention discloses application of a compound shown in a formula I in promoting in-vitro cultured oocyte development or in-vitro embryo development, and in the formula I, R1 is selected from halogenated alkyl; r2 is selected from H, halogen or C1-6 alkyl; n is selected from 0, 1, 2 or 3. According to the application, the expansion area of cumulus cells in the cumulus-oocyte complex can be increased; the oxidative damage of the cumulus-oocyte complex is relieved; the first polar body discharge rate of the oocytes is improved; the abnormal distribution rate of mitochondria in oocytes is reduced; the membrane potential of mitochondria in the oocyte is improved; and the cleavage rate and blastocyst formation rate of fertilized eggs can be improved, and the apoptosis rate of oocytes can be reduced.

Cumulus cell (CC) gene expression is explored as an additional method of morphological scoring to select an embryo with the highest chance of pregnancy. The present invention relates to a novel method for identifying biomarker genes for assessing the ability of mammalian oocytes to produce viable pregnancy after fertilization based on the use of live birth and embryonic development as endpoint criteria for the oocytes for exon level analysis of potential biomarker genes. The invention further provides biomarker genes of CC expression thus identified, as well as prognostic models based on biomarker genes identified using the methods of the invention.

Provided herein are methods for improving the efficiency of in vitro fertilization of animals. The method comprises (a) obtaining an oocyte from the animal, wherein the oocyte comprises an oocyte body and a zona pellucida; (b) obtaining sperm from the animal; (c) maturing the oocytes in a maturing medium; (d) removing cumulus cells from the oocytes; (e) drilling in the zona pellucida of the oocyte with a laser; (f) contacting the oocyte with the sperm; (g) co-incubating the oocytes with sperms, whereby the incubation enables in vitro fertilization of the oocytes and sperms to produce animal embryos, and whereby drilling is performed in the zona pellucida of the oocytes by laser to improve the efficiency of in vitro fertilization of the production of animal embryos.

The present invention provides a method for improving the efficiency of in vitro fertilization in animals. The method comprises: (a) obtaining oocytes from the animal, wherein the oocytes include the oocyte body and the zona pellucida; (b) obtaining sperm from the animal; (c) maturing the oocytes in a maturation medium; (d) removing cumulus cells from the oocytes; (e) creating holes in the zona pellucida of the oocytes with a laser; (f) contacting the oocytes with the sperm; and (g) incubating the oocytes with the sperm, wherein the incubation enables in vitro fertilization of the oocytes and sperm, and an animal embryo is produced; and the efficiency of in vitro fertilization for producing an animal embryo is improved by creating holes in the zona pellucida of the oocytes with a laser. In certain embodiments, the cumulus cells are removed from the oocytes by treatment with hyaluronidase, pipetting, and / or vortexing. In one embodiment, the oocytes are placed on a microscope stage prior to step (e).

The invention discloses a method for predicting OPU-IVF blastocyst development and embryo production performance by identifying a multi-gene expression pattern in an oocyte and a cumulus cell, which comprises the following steps: detecting expression patterns of mitochondrial functional genes ISCU and IGF2BP3 in the oocyte and an oocyte-cumulus cell interaction gene CTNND1; the method further comprises the step of detecting expression modes of an oxidative phosphorylation and metabolism regulation pathway SOX4, a gene KLF12 participating in cell cycle control and apoptosis regulation, an oxidative phosphorylation and metabolism regulation pathway OGT and an oocyte marker FST in cumulus cells. The expression modes of the genes can predict the embryonic development potential of oocytes after in-vitro maturation and in-vitro fertilization, important genetic information prediction is provided for predicting the reproductive performance of livestock, especially applying the in-vitro fertilization technology to embryo production of large livestock, and the breeding and breeding process of high-value livestock populations is promoted.