96 results about "Sperm injection" patented technology

A microinjection device is provided that includes an injection element defining a longitudinal axis, and that further includes a motor. The injection element is rotatable about the longitudinal axis by the rotational motor. The injection element is for penetrating a target, such as a cell. A microinjection system is provided that includes the microinjection device and a control unit. The control unit is for controlling a rotational amplitude and a frequency of oscillation of the injection element. A method for penetrating a target to facilitate injecting material therein is provided that includes providing the material to an injection element, contacting the target with a distal end of the injection element, rotating the injection element about a longitudinal axis to form a hole in the target, and penetrating the target with the injection element via the hole formed in the target. A method for performing intra-cytoplasmic sperm injection is provided that includes providing a solution comprising sperm to an injection element, contacting an oocyte with a distal end of the injection element, rotating the injection element alternately clockwise and counterclockwise about a longitudinal axis to form a hole in the oocyte, penetrating the oocyte with the distal end of the injection element via the hole formed in the oocyte, and expelling the solution comprising sperm into the penetrated oocyte.

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF) / intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices

A device for transferring cells present in fluid media is provided which is especially useful for in vitro fertilization techniques and intracytoplasmic sperm injection procedures.

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF) / intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices.

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.

A method for automated, artificial-intelligence-based COC retrieval includes using an artificial intelligence / machine learning system (AI / ML system) to optically scan a follicular fluid sample to produce an image object using an imaging system that includes a microscopy system, a camera system, and a lighting system. The method includes comparing the image object to a predetermined threshold using an AI / ML system, wherein the predetermined threshold is at least in part an optical pattern with a probability of corresponding to a cumulus-oocyte-complex (COC). The method includes identifying a COC within the follicular fluid sample based at least in part on the image object satisfying the predetermined threshold. The method includes determining a COC location within the follicular fluid sample based at least in part on identifying a region of the image object corresponding to an optical pattern within the image object that satisfies the predetermined threshold.

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. It may be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1 like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

The invention relates to a recombinant human granulosa cell enzyme solution and a preparation method of same and belongs to the technical field of artificial assisted reproduction. The recombinant human granulose cell enzyme solution is prepared by dissolving recombinant human granulosa cell enzyme dry powder, which is obtained by processing ovum, in a salt solution containing antibiotics and an indicator. In the invention, by means of a physiological work solution, which is prepared by means of non-animal-sourced recombinant bio-engineering high-tech hyaluronidase (a.k.a. recombinant human granulosa cell enzyme) dry powder, is 80 units (I.U.) and has functional available concentration, not only are granule cells surrounding the ovum effectively digested, which is beneficial to introcytoplasmic sperm injection (ICSI) technology of the ovum, but also possibility of animal-sourced pathogen and toxin are effectively excluded, thereby greatly improving safety of assisted reproduction.

The invention provides a micro fertilization method, which mainly comprises the following steps of: 1) taking fresh seminal fluid or frozen thawed seminal fluid; 2) taking fresh oocyte cells after the human chorionic gonadotropin (HCG) is injected for 14 to 18 hours; 3) respectively injecting the sperm in the fresh seminal fluid and the live sperm in the frozen thawed seminal fluid into the oocyte cells obtained in the second step to obtain injection oocyte; 4) activating the injection oocyte by using 5mumol / L ionomycin and 2mmol / L 6-dimethyaminopurine (6-DMAP) to obtain fertilized ovum; and 5) placing the fertilized ovum into culture liquid consisting of TCM199, 10 percent fetal bovine serum (FBS), 1.25 mM sodium pyruvate and 0.1 mM ethylene diamine tetraacetic acid (EDTA) to be cultured. The oocyte cell cytoplasm introcytoplasmic sperm injection method provided by the invention is simple and has the advantages that the operation is easy, and in addition, the large-scale production and popularization is convenient.

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. Itmay be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1-like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

The invention relates to the field of assisted reproduction, and discloses a composition for promoting sperm activation and a sperm activation solution. The sperm activation solution comprises a basicculture solution and a composition, wherein the composition comprises 2-10mg / L of ATP sodium salt, 5-15 U / mL of heparin, 5-10 [mu]g / mL of spot adhesion protein, 1-2.5[mu]g / mL of allene oxide synthase, 4-4.5 [mu]g / mL of acetyl coenzyme A acetyltransferase 2, 0.9-1.1 mmol / L of phosphatidylserine, 5.5-10.5 [mu]g / mL of coenzyme Q10, 3.5-6.5 ng / mL of linolenic acid and 0.45-0.5 [mu]g / mL of recombinantheat shock protein HSPA8. The invention also discloses a preparation method for the sperm activation solution. According to the sperm activation solution, the time of sperm injection and ovum in-vitro operation can be greatly shortened; the fertilization rate and the blastocyst formation rate are increased; the pregnancy rate is directly or indirectly increased; meanwhile, the sperm activation solution is effectively suitable for activation and optimization of sperms in an oocyte intracytoplasmic single sperm microinjection technology (ICSI) and an in-vitro fertilization (IVF) process in an in-vitro fertilization assisted reproduction technology.

The present invention relates to a long acting biologically active luteinizing hormone (LH) compound comprising an LH agonist linked to a pharmaceutically acceptable molecule providing an in vivo plasma half-life of the LH agonist or LH compound which is increased substantially compared to the in vivo plasma half-life of an LH agonist administered in the same manner as the LH compound. The present invention relates to methods for controlled ovarian stimulation which can be used in conjunction with assisted reproduction technologies such as in vitro fertilisation, intra cytoplasmatic sperm injection, intra uterine insemination and in vitro maturation. In other aspects the invention relates to methods for inducing folliculogenesis and methods for providing luteal support for the corpora lutea.

An intracytoplasmic sperm injection support device having a simple device configuration is provided. [Solution] An intracytoplasmic sperm injection support device 2 that supports intracytoplasmic sperm injection by injecting sperm held in a sperm injection tool 3 into an egg held by an egg holding device, comprising: an acquisition unit that acquires information including the relative positional relationship between the sperm injection tool 3 and the egg; and a control unit that controls the movement of a stage 11 on which the egg holding device is positioned to inject sperm into the egg and perform intracytoplasmic sperm injection, based on the information acquired by the acquisition unit.

A method for automated, artificial-intelligence-based egg identification using optical coherence tomography (OCT) includes positioning an OCT imaging system head in proximity to a biological sample containing an oocyte, wherein the OCT is operatively coupled to an artificial intelligence / machine learning system (AI / ML system) and an imaging system, wherein the imaging system includes a camera system, and a lighting system. The method includes creating at least one three-dimensional image of the oocyte using the OCT, AI / ML system, and imaging system. The method includes using the AI / ML system to analyze the three-dimensional image, wherein an analysis includes detection of a polar body's presence or absence based at least in part on planar views of the oocyte.

The present disclosure generally relates to micromanipulation devices and methods for the manipulation of mammalian sub-cellular structures. Uses include intra cytoplasmic sperm injection procedures, embryo biopsy procedures, trophectoderm biopsy procedures, mitochondrial transfer procedures, pronuclear transfer procedures, maternal spindle transfer procedures, cumulus cell biopsy procedures and other sub-cellular manipulation procedures. Devices comprise a cellular manipulation dish further comprising multiple sub- cellular manipulation units having a microwell and a cell deposit well wherein the cell deposit opening is disposed adjacent to the microwell opening.

The invention relates to a polyvinylpyrrolidone solution and a preparation method thereof, and belongs to the field of assisted reproductive technology (ART). The polyvinylpyrrolidone solution provided by the invention is prepared by adding taurine, serine, threonine, glycine, proline, chelating agent EDTA and polyvinylpyrrolidone into a salt solution. Not only the integrity of sperm plasma membranes can be effectively maintained, but also EDTA can chelate calcium ions, reduce the loss of calcium ions during self-motion removal of sperms, in order to improve the fertilization rate of sperm injection, and also provide a strong guarantee for the safety of assisted reproductive technology.

Co-injection of sperm heads and exogenous nucleic acid encoding the transgene into unfertilized mouse oocytes resulted in embryos expressing the transgene, reflecting the combination of nucleic acid and sperm heads before co-injection. Embryos obtained by co-injection were non-selectively transferred to surrogate mothers, producing offspring expressing the integrated transgene.

The invention discloses a method for carrying out ICSI (intracytoplasmic sperm injection) on a pig. The method for carrying out ICSI on the pig comprises the following steps: (1) hydrolyzing sperms in vitro animal semen with lipoidase or lipoidase solution, and collecting a hydrolysis product, so that the sperms used for preparing ICSI fertilized ova; (2) injecting the sperms used for preparing the ICSI fertilized ova into in vitro oocytes to obtain ICSI ova; and (3) carrying out electric activation on the ICSI ova and cultivating, so that the ICSI fertilized ova are obtained. The method for carrying out ICSI on the pig has the advantages that bad effects on depolymerization of pig sperms, formation of male pronucleus and later development due to addition of PVP can be avoided, and regions such as plasma membranes and perforatorium of the pig sperms can not be damaged, so that release of oocyte activation promoting factors from the pig subjected to ICSI operation can be facilitated, and the formation rate of the male pronucleus and the formation rate of blastocysts of embryos after fertilization are increased.

The present disclosure provides a microscopic insemination support device having a simple configuration. The present disclosure relates to a microscopic insemination support device that supports microscopic insemination in which sperm held by a sperm injection tool is injected into an egg held by an egg holding device. The microscopic insemination support device comprises an acquisition unit configured to acquire information including a relative positional relationship between the sperm injection tool and the egg, and a control unit configured to, on the basis of the information acquired by the acquisition unit, control movement of a stage on which the egg holding device is disposed so as to perform microscopic insemination by injecting sperm into the egg.

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence / machine learning system (AI / ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI / ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

The invention belongs to the field of medicine, and discloses application of a histone H3K79 methyltransferase inhibitor in improving the development efficiency of animal round sperm injection embryos. The invention discloses the application of the histone H3K79 methyltransferase inhibitor in improving the development efficiency of the animal round sperm injection embryos for the first time. Compared with an existing round sperm injection technology, the application has the advantages that under the condition that the transgenic risk is not introduced, the blastocyst development rate of the round sperm injection embryos can be remarkably increased through the histone H3K79 methyltransferase inhibitor, a good round sperm injection embryo in-vitro culture system is established, and the feasibility of the round sperm injection technology is improved.

The invention relates to an automatic ICSI system capable of accurately identifying, which comprises a power supply module, a main control module, a sample optical observation module, a user interaction module, a sperm capturing unit, an ovum fixing unit and a single sperm injection unit, the sample optical observation module, the user interaction module, the sperm capturing unit, the ovum fixing unit and the single sperm injection unit are all electrically connected with the main control module, and the operation method of the system comprises three steps of sperm capturing, ovum fixing and single sperm injection. According to the automatic ICSI system capable of accurately identifying and the operation method, the system can replace manual work to realize automation, is stable and reliable in experimental structure, adopts an AI image system to identify healthy and high-quality sperms and intelligently identify the spindle body part of an ovum, can perform needle insertion and injection on the best-quality sperms through the most appropriate position, greatly reduces the damage to the ovum, and the probability of various fertilization failures or embryo development potential underground caused by human factors is reduced to the greatest extent.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

A method for automated ICSI includes receiving at least one droplet containing an egg in a dish placed on a stage. The method includes using an artificial intelligence / machine learning system (AI / ML system) and an imaging system to detect a zona pellucida. The imaging system includes a microscopy system, a camera system, and a lighting system. The method includes holding the egg using a robotic microtool and lowering a robotic pipettor into the droplet. The method includes using the AI / ML system and imaging system to determine an area at which to hold the egg and positioning the robotic microtool to that area. The method includes using the AI / ML system and imaging system to instruct the robotic microtool to apply negative pressure to hold the egg to the robotic pipettor. The method includes using the AI / ML system and imaging system to determine a target location where zona ablation should be performed.

To provide a sperm selection aid that enables the selection of good quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. [Solution] A sperm sorting assistance device comprising: an index calculation unit that calculates at least one index related to the quality of sperm recognized in a video; an evaluation unit that relatively evaluates the quality of first sperm recognized in a first time interval in a video and second sperm recognized in a second time interval different from the first time interval in a video, based on at least one index; and a display control unit that displays information relating to at least one index of the first sperm to the first sperm in the first time interval in a video, and displays the relative quality evaluation of the first sperm and the second sperm evaluated by the evaluation unit.

The invention relates to the technical field of medical instruments, in particular to a surgical device for artificial insemination in reproductive medicine, which comprises a syringe and a support, a control panel and a hose fixed at the top of the syringe are fixedly connected to the outside of the support, and semen injection holes are uniformly formed in the top end of the hose; the suction assembly is fixedly mounted on the syringe and is used for sucking semen; the supporting assembly is movably arranged outside the hose and used for assisting the hose in entering the vagina; according to the scheme, through the fixed connection mode of the hose and the syringe, the operation steps are simplified, through mutual cooperation of the first piston, the first connecting rod, the filter membrane and other structures, seminal fluid can be adsorbed into the syringe through the suction assembly, then seminal fluid outflow caused by loosening of a connector can be avoided, the operation success rate is further increased, and the pregnancy rate of a patient is further guaranteed.

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample on a staging mechanism, wherein the staging mechanism includes at least one motor to position the vessel at a specified orientation, using an artificial intelligence / machine learning (AI / ML system) system. The method includes confirming a position of the vessel, using the AI / ML system. The method includes directing a robotic pipettor to place a pipette tip within the semen sample at a position specified by the AI / ML system. The method includes aspirating and mixing the semen sample using the robotic pipettor. The method includes receiving an image object from an imaging system that includes a microscopy system, a camera system, and a lighting system. The method includes determining a semen liquefaction state of the semen sample based at least in part on the image object, using the AI / ML system.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.

The invention belongs to the field of medicines, and discloses application of a histone methyltransferase inhibitor to improvement of animal round sperm injection embryo development efficiency. The invention discloses the application of the histone methyltransferase inhibitor in improving the animal round sperm injection embryo development efficiency for the first time. Compared with an existing round sperm injection technology, the blastocyst development rate, implantation rate and living yield of the round sperm injection embryo of the animal can be remarkably improved through the histone methyltransferase inhibitor under the condition that transgenic risks are not introduced; in addition, the round sperms treated by the histone methyltransferase inhibitor can be injected to generate the live F0 generation to normally breed offspring, a good round sperm injection embryo in-vitro culture system is established, and the feasibility of the round sperm injection technology is improved.

To provide an intracytoplasmic sperm injection support device with a simple device configuration. [Solution] Sperm held in sperm injection tool 3 is held by egg holding device An intracytoplasmic sperm injection (ICSI) support device 2 that assists in intracytoplasmic sperm injection (ICSI) in which sperm is injected into an egg, comprising a sperm injection tool 3 and an egg An acquisition unit that acquires information including the relative positional relationship with the child, and the information acquired by the acquisition unit Based on this, an egg retention device is positioned to perform intracytoplasmic sperm injection (ICSI) by injecting sperm into the egg. An intracytoplasmic sperm injection support device 2 comprises a control unit that controls the movement of stage 11.