35 results about "Sperm injection" patented technology

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. It may be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1 like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

The invention relates to a recombinant human granulosa cell enzyme solution and a preparation method of same and belongs to the technical field of artificial assisted reproduction. The recombinant human granulose cell enzyme solution is prepared by dissolving recombinant human granulosa cell enzyme dry powder, which is obtained by processing ovum, in a salt solution containing antibiotics and an indicator. In the invention, by means of a physiological work solution, which is prepared by means of non-animal-sourced recombinant bio-engineering high-tech hyaluronidase (a.k.a. recombinant human granulosa cell enzyme) dry powder, is 80 units (I.U.) and has functional available concentration, not only are granule cells surrounding the ovum effectively digested, which is beneficial to introcytoplasmic sperm injection (ICSI) technology of the ovum, but also possibility of animal-sourced pathogen and toxin are effectively excluded, thereby greatly improving safety of assisted reproduction.

The invention provides a micro fertilization method, which mainly comprises the following steps of: 1) taking fresh seminal fluid or frozen thawed seminal fluid; 2) taking fresh oocyte cells after the human chorionic gonadotropin (HCG) is injected for 14 to 18 hours; 3) respectively injecting the sperm in the fresh seminal fluid and the live sperm in the frozen thawed seminal fluid into the oocyte cells obtained in the second step to obtain injection oocyte; 4) activating the injection oocyte by using 5mumol/L ionomycin and 2mmol/L 6-dimethyaminopurine (6-DMAP) to obtain fertilized ovum; and 5) placing the fertilized ovum into culture liquid consisting of TCM199, 10 percent fetal bovine serum (FBS), 1.25 mM sodium pyruvate and 0.1 mM ethylene diamine tetraacetic acid (EDTA) to be cultured. The oocyte cell cytoplasm introcytoplasmic sperm injection method provided by the invention is simple and has the advantages that the operation is easy, and in addition, the large-scale production and popularization is convenient.

The invention relates to a polyvinylpyrrolidone solution and a preparation method thereof, and belongs to the field of assisted reproductive technology (ART). The polyvinylpyrrolidone solution provided by the invention is prepared by adding taurine, serine, threonine, glycine, proline, chelating agent EDTA and polyvinylpyrrolidone into a salt solution. Not only the integrity of sperm plasma membranes can be effectively maintained, but also EDTA can chelate calcium ions, reduce the loss of calcium ions during self-motion removal of sperms, in order to improve the fertilization rate of sperm injection, and also provide a strong guarantee for the safety of assisted reproductive technology.

Co-injection of sperm heads and exogenous nucleic acid encoding the transgene into unfertilized mouse oocytes resulted in embryos expressing the transgene, reflecting the conjugation of the nucleic acid and sperm heads prior to co-injection. Non-selective transfer of coinjected embryos into surrogate mothers yielded offspring expressing the integrated transgene.

The invention relates to an automatic ICSI system capable of accurately identifying, which comprises a power supply module, a main control module, a sample optical observation module, a user interaction module, a sperm capturing unit, an ovum fixing unit and a single sperm injection unit, the sample optical observation module, the user interaction module, the sperm capturing unit, the ovum fixing unit and the single sperm injection unit are all electrically connected with the main control module, and the operation method of the system comprises three steps of sperm capturing, ovum fixing and single sperm injection. According to the automatic ICSI system capable of accurately identifying and the operation method, the system can replace manual work to realize automation, is stable and reliable in experimental structure, adopts an AI image system to identify healthy and high-quality sperms and intelligently identify the spindle body part of an ovum, can perform needle insertion and injection on the best-quality sperms through the most appropriate position, greatly reduces the damage to the ovum, and the probability of various fertilization failures or embryo development potential underground caused by human factors is reduced to the greatest extent.

The invention belongs to the field of medicines, and discloses application of a histone methyltransferase inhibitor to improvement of animal round sperm injection embryo development efficiency. The invention discloses the application of the histone methyltransferase inhibitor in improving the animal round sperm injection embryo development efficiency for the first time. Compared with an existing round sperm injection technology, the blastocyst development rate, implantation rate and living yield of the round sperm injection embryo of the animal can be remarkably improved through the histone methyltransferase inhibitor under the condition that transgenic risks are not introduced; in addition, the round sperms treated by the histone methyltransferase inhibitor can be injected to generate the live F0 generation to normally breed offspring, a good round sperm injection embryo in-vitro culture system is established, and the feasibility of the round sperm injection technology is improved.

A sperm injection tube for artificial insemination of swine has a tube and a plug mounted on an end of the tube. The plug has a plug body, a guiding portion, and a blocking portion. The guiding portion is formed around the plug body and has multiple guiding fins near the injection hole. The blocking portion is formed around the plug body and has at least one blocking fin near the connecting hole. Each guiding fin is umbrella-shaped and flexible. When inserting the sperm injection tube into the uterus, the guiding fins are concentrated toward the plug body to led the plug to easily pass through the cervix. The blocking portion block the cervix to prevent the semen flowing out and to keep the tube from being pushed out to raise the conception rate of the sow.

A method of enhancing embryo implantation in a subject is disclosed which comprises administering to the uterine cavity of the subject a formulation comprising copper and/or zinc in an amount effective to stimulate endometrial production of leukaemia inhibitory factor (LIF) and/or vascular endothelial growth factor (VEGF). Alternatively, a device may be inserted into the uterine cavity of the subject, wherein the device comprises copper and/or zinc, for a period of time that is effective to stimulate endometrial production of LIF and/or VEGF. The method is suitable for use with women undergoing treatment by any of the assisted reproductive technologies, such as those involving the transfer of embryos such as in vitro fertilisation (IVF) and variants including IVF-ICSI (intracytoplasmic sperm injection) and in vitro maturation (IVM) treatments, as well as intrauterine-insemination (IUI) therapy. However, the method is also applicable for women wanting to improve their prospects of pregnancy through natural conception.

The invention belongs to the technical field of medicines and relates to a sperm freezing method based on ICSI micromanipulation. The method comprises the following steps: (1) preparing two ICSI dishes, introducing sperm samples into one ICSI dish, and introducing one or multiple drops of sperm freezing protecting liquid into the other ICSI dish; (2) extracting motile sperms from the ICSI dish containing the sperm samples by virtue of a single sperm injection needle, and introducing the motile sperms into liquid drops in the sperm freezing protecting liquid in the other ICSI dish; and (3) putting the ICSI dish containing the liquid drops of the sperm freezing protecting liquid into liquid nitrogen, freezing, and carrying out preservation. The method has the beneficial effects that a carrier is saved, the intermediate links of a small amount of sperms in the freezing and unfreezing processes are remarkably reduced, the activity ratio of the unfrozen sperms is high, the loss rate is low,and the utilization rate of the sperms is high.

The invention discloses a follicle puncture detection method based on near-infrared vision. The follicle puncture detection method comprises the following steps: firstly, grabbing a follicle punctureoperation surface by a near-infrared camera, wherein the operation surface comprises a follicle to be injected and an injection tube carrying sperms, and realizing injection tube calibration accordingto the fact that the injection tube carrying the sperms is a unique moving object in the operation surface; and determining an intersection point with follicular zona pellucida along the moving direction of the calibrated injection tube, determining an expected position of a position identifier according to the intersection point, and finally completing detection according to whether the positionidentifier appears at the expected position. Position identification is set in a self-adaptive mode according to the light and shade difference of gray level images of the zona pellucida, substancesin the zona pellucida, the sperms in the injection needle and seminal plasma under near-infrared light, follicle puncture is judged to be successful when the position identification appears in the visual field, and automatic control injection is achieved. In an in-vitro fertilization and embryo transplantation combined technology, the puncture effect can be effectively stabilized and the success rate of one-time puncture injection can be improved by means of injecting single sperms in egg pulp.

A method for preserving spermatozoa (sperm) by air-drying. A semen sample is received and the sperm are separated from contaminants in the semen sample. The sperm may be separated from the semen sample by layering the semen sample onto a two-layer discontinuous percoll layer followed by centrifugation or by centrifuging the semen sample. The sperm concentrate is spread onto a sterile plate and allowed to air dry. The air-dried sperm may be stored at 0° C. to 8° C. The air-dried sperm may be recovered by adding a warm physiological solution to the air-dried sperm. The recovered sperm may be used for in vitro fertilisation or for intracystoplasmic sperm injection (ICSI).

The invention belongs to the field of medicines, and discloses application of a DNA methyltransferase inhibitor to improvement of animal round sperm injection embryo development efficiency. The invention discloses the application of the DNA methyltransferase inhibitor in improving the development efficiency of the animal round sperm injection embryo for the first time; compared with an existing round sperm injection technology, the blastocyst development rate of the round sperm injection embryo can be remarkably improved through the DNA methyltransferase inhibitor under the condition that transgenic risks are not introduced; furthermore, excellent round sperm injection embryo in-vitro culture system is established, and the feasibility of a round sperm injection technology is improved.