Recombinant human granulosa cell enzyme solution and preparation method of same

A technology of granule cells and enzyme solution, which is applied in the field of recombinant human granule cell enzyme solution and its preparation, can solve the problems of high cost and deterioration, and achieve the effects of improving safety and eliminating the possibility of animal-derived pathogens and toxins

Pending Publication Date: 2017-07-04
瑞柏生物(中国)股份有限公司
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Problems solved by technology

[0004] The hyaluronidase solution in my country's assisted reproductive technology is almost completely dependent on imported finished products. Not only is the cost high, but also the effectiveness of t

Method used

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Embodiment Construction

[0026] The present invention will be further described below in combination with specific implementation methods.

[0027] The recombined human granulocyte enzyme solution of the present invention is prepared by dissolving the dry powder of recombined human granulocyte enzyme for treating ovum in a saline solution containing antibiotics and indicators.

[0028] The antibiotic is gentamicin with a concentration of 5-11 μg / ml; the indicator is phenol red with a concentration of 9.0-10.0 μg / ml.

[0029] The salt solution is a compound solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it includes: sodium chloride 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, magnesium sulfate 0.18-0.22mmol / L, calcium chloride 1.92-2.12mmol / L, sodium bicarbonate 3.90-4.10mmol / L, buffer 19.99- 22.00mmol / L, glucose 2.68-2.88mmol / L, sodium pyruvate 0.31-0.33...

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Abstract

The invention relates to a recombinant human granulosa cell enzyme solution and a preparation method of same and belongs to the technical field of artificial assisted reproduction. The recombinant human granulose cell enzyme solution is prepared by dissolving recombinant human granulosa cell enzyme dry powder, which is obtained by processing ovum, in a salt solution containing antibiotics and an indicator. In the invention, by means of a physiological work solution, which is prepared by means of non-animal-sourced recombinant bio-engineering high-tech hyaluronidase (a.k.a. recombinant human granulosa cell enzyme) dry powder, is 80 units (I.U.) and has functional available concentration, not only are granule cells surrounding the ovum effectively digested, which is beneficial to introcytoplasmic sperm injection (ICSI) technology of the ovum, but also possibility of animal-sourced pathogen and toxin are effectively excluded, thereby greatly improving safety of assisted reproduction.

Description

technical field [0001] The invention relates to a recombinant human granulocyte enzyme solution and a preparation method thereof, belonging to the technical field of artificial assisted reproduction. Background technique [0002] Hyaluronidase (hyaluronidase) is a general term for enzymes that can reduce the molecular weight of hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability of tissues. Hyaluronidase can effectively digest the hyaluronic acid between the granulosa cells, so that the granulosa cells around the eggs can be mechanically removed through the egg peeling pipette (needle), followed by single sperm injection or egg freezing techniques. [0003] In the prior art, the conventional hyaluronidase is mainly derived from the testicular tissue of animals, such as cattle and sheep, and there is a potential risk of animal-derived pathogens and toxins, which brings safety problems to assist...

Claims

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Application Information

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IPC IPC(8): C12N9/26
CPCC12N9/2474C12Y302/01035
Inventor 冯怀亮冯博王学强
Owner 瑞柏生物(中国)股份有限公司
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