43 results about "Testicular tissue" patented technology

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

Disclosed herein are methods of reducing pathological inflammation and effects thereof through administration of tissues associated with regenerative and / or tolerogenic activity. In some embodiments, such tissues can include placental, ocular, testicular, and osseous tissues. Augmentation of therapeutic effects of said tissues may be achieved through contact with Injury Mimetics.

The invention belongs to the technical field of medicines, and particularly discloses application of afzelin in preparation of an anti-testis aging medicament, and testis aging resistance is particularly improvement or recovery of an aged testis tissue structure. The invention also provides a medicament for resisting testis aging. The medicament contains afzelin. The invention provides a new application of afzelin, the afzelin can be used for saving senescence testicular tissue structure disorder, remarkably reducing the epididymal sperm deformation rate and increasing the number of supporting cells and undifferentiated spermatogonium cells, the new application value of afzelin is developed, and a theoretical basis is provided for developing afzelin into anti-reproductive aging drugs.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

An embodiment relates to polydeoxyribonucleotide (PDRN)-containing exosomes, a composition including same, and a preparation method therefor, wherein the PDRN-containing exosomes of the embodiment are derived from a fish testis tissue and include high-purity, low-molecular weight PDRN. In addition, exosomes containing a significant amount of low molecular weight PDRN can be induced through a simple process of ultrasonic treatment. Furthermore, application of ultrasound to the separated exosomes can prepare PDRN exosomes that are made to have low molecular weights, whereby the exosomes exhibit high intracellular uptake rates and thus can be advantageously used for manufacturing a tissue regeneration or anti-inflammatory composition.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The invention belongs to the technical field of reproductive biology, and particularly provides a method for separating and culturing testicular perichondrial muscle-like cells of an adult mouse. The method comprises the following steps: removing interstitial cells from testis tissues of an adult mouse, and digesting the testis tissues by pancreatin and DNA enzyme to obtain a testis single-cell suspension; and culturing by adopting a new isolated culture medium until the culture dish is full of cells, and carrying out cell passage, cell cryopreservation and resuscitation and cell identification by adopting passage culture mediums with different concentrations. According to the method for separating and culturing the mouse peridermoid cells, the cell separation degree is far higher than that in the prior art, operation is easy, time is saved, the technical blank that the mouse peridermoid cells are difficult to separate and culture is filled, and the method is suitable for large-scale popularization and application. And convenience is provided for research on interaction of the mouse testicular peritubular muscle-like cells with other testicular somatic cells and germ cells.

The invention discloses application of a xanthoceras sorbifolia extract in preparation of a medicine for preventing and / or treating electromagnetic radiation damage. Experiments show that the xanthoceras sorbifolia bunge flower extract can improve the weight loss of rats caused by microwave radiation; brain tissue structure change damage caused by microwave radiation is improved; the reduction of the learning and memory ability of the rats caused by microwave radiation is improved; the brain sea horse tissue amino acid neurotransmitter disorder caused by microwave radiation is improved; electroencephalogram alpha and beta wave abnormity caused by microwave radiation is improved; the sperm motility reduction caused by microwave radiation is improved; the rat serum sex hormone disorder caused by microwave radiation is improved; rat testicular tissue oxidative stress injury caused by microwave radiation is improved; the abnormal energy metabolism of testicular tissues caused by microwave radiation is improved; heart electrophysiological abnormality and heart tissue ultrastructure abnormality caused by microwave radiation are improved; the immune function decline caused by microwave radiation is improved; the influence of microwave radiation on the content of IL-2, 4 is improved; the influence of microwave radiation on the thymus and spleen tissue structure is improved.

The invention provides a gene influencing sexual precocity of deep-county pigs and a screening method thereof, and belongs to the field of research on sexual maturity of pigs, the gene influencing sexual precocity of deep-county pigs comprises an SRD5A1 gene and / or a CYP11B2 gene, testicular tissues of deep-county pigs before and after sexual maturity are subjected to transcriptome sequencing, differential expression gene functions are analyzed, and the sexual precocity of deep-county pigs before and after sexual maturity is obtained. And screening out candidate genes related to the sexual maturity of the deep county pigs. And then performing transcriptome sequencing on testis tissues of the sexually mature deep county pig and the hybrid pig of the sexually mature deep county pig and the large white pig, analyzing functions of differential expression genes, and screening specific expression genes of the sexual maturity of the deep county pig. Integrating the results of the two times of transcriptome sequencing, analyzing the functions of the coincident genes, and screening candidate genes SRD5A1 and CYP11B2 which influence the sexual precocity of the pigs in the deep county according to the gene functions; and a foundation is laid for subsequent research on precocious puberty of pigs in deep county.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The invention relates to the technical field of poultry reproductive biology, and particularly discloses a phosphorylated protein marker for fasting-induced chicken blood testis barrier remodeling, a screening method and application. The marker comprises a Ser1573 phosphorylation site which is tightly connected with the protein ZO-1 and a Ser1620 phosphorylation site of the ZO-1. The screening method comprises the following steps: collecting testicular tissues of cocks at key time points of fasting and recovering foraging, carrying out proteomics and phosphorylation detection, analyzing and screening different phosphorylation sites in combination with bioinformatics, and carrying out multi-dimensional verification through Western blot, immunofluorescence and cell function experiments. The invention provides a key molecular marker for evaluating the testis functional state of the cock and optimizing a limited feeding scheme in breeding of the breeder cock, and has important production and application values.

The invention discloses an application of curcumin in improving puberty male reproductive capacity damage caused by chemotherapeutic drugs. Aiming at the problems of testicular germ cell quantity reduction, oxidative stress injury, long-term fertility damage and the like caused by cytosine arabinoside chemotherapy, curcumin is applied while or before the cytosine arabinoside is applied. The application is realized through combined administration or a pharmaceutical composition containing curcumin and cytarabine. Experiments show that the scheme can effectively relieve testicular tissue form damage, reduce germ cell apoptosis and antagonize oxidative stress, and significantly improve the sperm quality of adult individuals. The invention provides a new potential strategy for synchronously protecting the reproductive function of a clinical child tumor patient during chemotherapy.

The invention relates to the technical field of medicines, and discloses an application of lupin or an extract thereof in preparation of a drug for relieving reproductive toxicity of chemotherapeutic drugs, the lupin is selected from lupin plants, preferably lupin variety Jenabillup, and the chemotherapeutic drugs comprise busulfan, cyclophosphamide or cis-platinum. The administration dosage of the lupin or the extract thereof is 0.1-100 mg / kg body weight, the administration period is continuous administration for at least 5 weeks, experiments show that the lupin can significantly improve the sperm concentration and sperm motility of chemotherapy model animals and improve pathological damage of testicular tissues, the mechanism relates to antioxidant, anti-inflammatory and anti-apoptosis effects, and the lupin or the extract thereof can be used for preparing a chemotherapy model animal. The invention provides a safe and effective new strategy for fertility protection of chemotherapy patients.

The invention discloses a method for improving inhibition of slow heat stress on male rabbit breeding, which is executed by a computer system and comprises the following steps: acquiring environmental data and physiological indexes such as male rabbit ear temperature and respiratory rate in real time through a multi-modal sensor; the core algorithm constructs a slow heat stress cumulative damage model containing a time decay factor, and quantifies the integral effect of the heat load and the body repair process. The system generates multi-dimensional optimal control vectors of environment cooling, nutrition intervention and LED red light repair through a physiology-environment coordinated regulation and control model on the basis of an accumulated damage index generated by the model. According to the method, the limitation of traditional single threshold value control is broken through, and full-period precise prevention and control of physical cooling open circuit, nutrition restoration follow-up and illumination rehabilitation ending is achieved. Experiments prove that the scheme can effectively protect testis tissues, shorten the reproductive function recovery period and improve the semen quality, the breeding and medicament cost is reduced while the reproductive performance is guaranteed, and the method is suitable for different scales of rabbit farms.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The invention relates to the technical field of animal science and animal production, in particular to application of melatonin in preparation of a medicine for relieving cadmium-induced pig testicular tissue injury. Cadmium has testicular toxicity, which shows that cadmium exposure changes the transcriptional level of testicular genes, causes oxidative stress, activates inflammation and NF-kB signal channels, mediates abnormal iron metabolism, causes cell ferroptosis, and finally causes testicular tissue cell structure damage. It is found for the first time that an anti-oxidation defense system is remodeled to a certain extent through addition of melatonin, oxidative stress, inflammation and ferroptosis induced by cadmium exposure are inhibited, and testicular tissue cell structure damage is relieved. The invention provides a theoretical basis for relieving the damage of the environmental pollutant cadmium by using the melatonin in production practice.

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent for inhibiting PPAR alpha expression in preparation of a medicine for treating male infertility diseases, wherein a PPAR alpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the number of sperm in a Cd+GW6471 group is higher than that in a Cd+NAC group, the serum testosterone level in the Cd+GW6471 group is also higher than that in the Cd+NAC group, and the results of testicular tissue pathological staining also show that GW6471 can more significantly restore the decrease in the number of mature spermatogenic tubules caused by Cd, GW6471 restores the decrease in the number of SYCP3 positive spermatocytes caused by Cd, so that the sperm production efficiency is restored, and therefore the application of the reagent for inhibiting PPAR alpha expression in preparation of the medicine for treating male infertility diseases is proposed.

The invention belongs to the technical field of culture media, and discloses a culture medium for in-vitro culture of cynomolgus monkey testicular tissue. Through an innovative culture technology and in combination with a culture formula of adding ANGPTL4 and IGF1, the survival rate and spermatogenesis efficiency of testis tissues are optimized, an efficient and reliable technical means is provided for reproductive medicine research and fertility preservation, and the method is particularly suitable for pre-adolescent individuals or wild protection animals or humans needing to preserve fertility potential. The innovative rotary culture technology and the culture scheme that 20 ng / mL of ANGPTL4 and 20 ng / mL of IGF1 are additionally supplemented are beneficial to the integrity of testicular tissues in the in-vitro culture process and promote proliferation and differentiation of germ cells, and the spermatogenesis efficiency is improved. And a feasible technical scheme and a new insight can be provided for the research of male animal in-vitro spermatogenesis.

The invention relates to the technical field of animal reproductive biology, and discloses a single-cell multi-omics method for analyzing a cattle testis development and spermatogenesis regulation mechanism, which comprises the following steps: (a) acquiring testis tissue samples of cattle at different development stages; (b) respectively carrying out single cell RNA sequencing (scRNA-seq) and single cell nucleus ATAC sequencing (snATAC-seq) on the testicular tissue sample, so as to obtain transcriptome data and chromatin accessibility data. According to the single-cell multi-omics method for analyzing the regulation and control mechanism of testis development and spermatogenesis of the cattle, by integrating single-cell RNA sequencing and single-cell nucleus ATAC sequencing, nine main cell types and specific marker genes (including newly found marker genes such as CPEB1, ADGRG1 and the like) of testis of the Simmental cattle from birth to puberty are systematically identified; the composition change rule of each cell type in different development stages is finely described, and a comprehensive cell map is provided for understanding the development of the bovine testis.

The invention relates to application of ginsenoside Rg4 as a unique active ingredient in preparation of a medicine for improving male delayed gonad hypofunction, belonging to the field of biological medicine. According to the invention, the action mechanism of the Rg4 for improving LOH is defined for the first time: the Rg4 can specifically up-regulate the mRNA expression quantity of StAR and StARD7 in a testis tissue, and promote the transport of cholesterol to the inner mitochondrial membrane of a testis Leydig cell from the transcriptional level; the Rg4 improves the mRNA expression quantity of P450scc, 3 beta-HSD in testicular tissues by promoting the efficient transport of cholesterol, enhances the conversion of cholesterol to pregnenolone and the catalytic synthesis of subsequent steroids, and improves the endogenous testosterone synthesis capability; the Rg4 can recover the negative feedback regulation function of an HPG axis, regulate secretion of pituitary LH and FSH to physiological steady state, and avoid abnormal fluctuation of hormone level. The invention fills the technical blank of Rg4 in the field of LOH gene regulation and control.

The invention belongs to the technical field of biology, and provides a vitrification rapid cryoprotectant for preserving pre-puberty male testis tissue and a freezing method aiming at the problems that an existing testis tissue preserving method is unstable in preserving effect, long in consumed time and complex in operation. The cryoprotectant comprises a cryoprotectant A, a cryoprotectant B and a cryoprotectant C, the cryoprotectant A comprises a DMEM (Dulbecco Modified Eagle Medium), fetal calf serum and ethylene glycol; the cryoprotectant B comprises a DMEM (Dulbecco Modified Eagle Medium), fetal calf serum and ethylene glycol; the cryoprotectant C comprises a DMEM (Dulbecco Modified Eagle Medium), fetal calf serum, cane sugar and ethylene glycol. According to the method, the pre-puberty male testicular tissue can be effectively preserved at low temperature, the high cell viability is obtained, and the structural integrity of the testicular tissue is well preserved.

This invention provides a method for isolating spermatogonial stem cells from frozen testicular tissue, belonging to the field of cell biology. The isolation method provided by this invention includes the following steps: thawing frozen testicular tissue; digesting the thawed tissue with collagenase IV and DNase I until the seminiferous tubules are exposed and their walls become rough; washing with DPBS buffer, centrifuging, and culturing until cells migrate; collecting the migrated cells and purifying them for spermatogonial stem cell enrichment. The method described in this invention can maximally preserve the internal environment of spermatogonial stem cells, maintain cell viability, and reduce cell damage, and can be used for isolating spermatogonial stem cells from frozen testicular tissue.

The invention discloses application of sodium 4-phenylbutyrate in a product for improving dyszoospermia of obese males, and belongs to the technical field of biological medicines. High-fat feed is adopted to construct a rat obesity model, the protection effect of 4-PBA on the reproductive function of obese rats is studied, and experimental results show that after the 4-PBA intervenes the obese rats, the testosterone content can be obviously increased, the estradiol level can be reduced, the sperm motility can be improved, the sperm malformation rate can be reduced, the spermatogenic dysfunction can be obviously relieved, and the anti-obesity effect on testis tissue morphology, testis tissue morphology, testis tissue morphology, testis tissue morphology and testis tissue morphology can be improved. The functions of supporting cell mitochondria, endoplasmic reticulum and MAM structures are obviously improved, and a new treatment strategy and a new drug target are provided for intervening and controlling obesity induced testis dyszoospermia and improving fertility of obese males.

The invention discloses a human immature testis in-vitro culture contamination model and a construction method thereof, and belongs to the technical field of biomedicine. According to the method, the human testis tissue is directly used and is closer to the physiological and toxicological response of the human body, the testis beside the benign testis tumor are taken, and a sample is easier to obtain; the culture at 34 DEG C better conforms to the physiological environment of the testis, and tissue degeneration at 37 DEG C is avoided; providing two models, namely 10 <-5 > M (4-day model, accelerated toxicity characterization for mechanism screening) and 10 <-7 > M (10-day model, prolonged contamination time and real reaction of time accumulation effect of toxicity); multi-level evaluation systems of morphology, molecular biology, transcriptomics and the like are integrated; the method can be used for testicular toxicity screening and mechanism research of other environmental toxicants.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The invention provides a mononuclear transcriptomics technology-based analysis method for interaction of testicular sertoli cells and germ cells of plateau livestock, and belongs to the technical field of bioinformatics. The analysis method comprises the following steps: carrying out mononuclear transcriptome sequencing by taking testis tissues of plateau livestock at different development stages as materials, dividing a supporting cell subset and a germ cell subset according to marker genes, drawing a quasi-time differentiation track, screening differential expression genes and enriching functions, and constructing a supporting cell and germ cell interaction molecular network. According to the method, molecular characteristics, differentiation tracks and interaction networks of supporting cells and germ cells of the testis of the Tibetan sheep in different development stages are analyzed, and the problems that in the prior art, cell resolution is insufficient, development dynamics are deficient, variety specificity information is deficient and the like are solved; a brand new technical approach and theoretical basis are provided for clarification of cell molecular basis of low reproductive capacity of Tibetan sheep and mining of key regulatory factors.

The application belongs to the field of animal reproductive biotechnology, and discloses application of support cell exosomes in promoting in-vitro culture of pig testis tissue, the in-vitro culture comprising: placing pig testis tissue pieces on a three-dimensional culture support and culturing by using a sequential medium system; wherein the sequential medium system comprises M1 medium used for initial culture and M2 medium used for subsequent culture, the M1 medium comprising StemPro-34 SFM basic medium, and the M2 medium comprising alpha-MEM basic medium. The technical problems of poor in-vitro culture effect of testis tissue and difficulty in maintaining the spermatogenesis process in the prior art are solved.