88 results about "Testicular tissue" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention provides a kit for detecting spermatogenic ability of azoospermia patients and application thereof, and belongs to the technical field of reproductive medicines.The kit comprises the following reagents: testis tissue digestive juice and testis single cell flow staining fluid; the testis single cell flow type staining solution comprises a DNA (Deoxyribose Nucleic Acid) staining solution Hoechst 33342 and an RNA (Ribonucleic Acid) staining solution # imgabs0 # RNASelectTM green fluorescent cell staining agent; according to the kit, double staining of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) is carried out on testis single cells, and a fluorescence activated cell sorting technique is matched, so that refining of testis single cell subgroups of azoospermia patients is realized, and the spermatogenic ability of the azoospermia patients is determined according to a testis single cell subgroup subdivision result. The kit disclosed by the invention is used for pre-operation evaluation of a microscopic sperm extraction operation, is used for judging whether a patient has spermatogenic ability and is suitable for microscopic sperm extraction or not, can also be used for guiding the patient to further clarify the cause of disease, and has important clinical significance.

The invention relates to the technical field of reproductive biology, in particular to a method for efficiently constructing pig testis organs in vitro. Comprising the following steps: S1, enzymolysis of testicular tissues of piglets; and S2, testicular cell resuspension counting: uniformly suspending the testicular cells obtained after enzymolysis in the step S1, sequentially collecting the cells into a 96-well plate with a U-shaped bottom, and adding a DMEM base culture medium containing 5% of FBS, 10% of KSR and 1% of P / S into each well to complete resuspension culture of the cells. S3, testicular organ in-vitro culture: separating testicular cell masses, transferring the separated cell masses into a 96-well plate with a U-shaped bottom, and adding the cell masses into a DMEM base culture medium containing 5% of FBS, 10% of KSR and 1% of P / S for culture; and the quantity of cells in the 96-well plate with the U-shaped bottom is 5 * 104 cells per well. After the culture system is optimized, the experimental process is simplified, the testicular organs can be directly cultured in the 96-well plate with the U-shaped bottom, the replacement frequency is reduced, the pollution risk is reduced, the number of adopted cells is remarkably reduced, and the culture efficiency is improved.

The invention relates to the technical field of reproductive medicine, in particular to a high-quality sperm acquisition method based on a testis tissue sample of an azoospermia patient, which comprises the following steps: inputting a first frame of a testis tissue sample microscopic video sequence into a target detection model, outputting a sperm detection frame and confidence, and taking the position of the detection frame as a starting point of a motion trail; a dynamic confidence matching strategy is adopted, firstly, a prediction trajectory is matched with a high-confidence detection result, then, a real leak detection target is complemented with a low-confidence target, multi-stage Hungary matching is achieved through confidence guidance, and all sperm movement trajectories obtained in each frame are obtained; and based on multiple frames of images corresponding to all sperm movement tracks corresponding to the microscopic video sequence, selecting the multiple frames of images according to a preset number of confidence coefficients to construct a candidate morphological analysis sample set, and performing kinematics parameter calculation and morphological analysis to obtain high-quality target sperms. According to the method, the recognition efficiency of high-quality sperms is improved, the sperms with higher quality can be obtained, and the subsequent fertilization success rate is further improved.

The invention discloses a porcine circular RNA, a porcine circLDHC, an overexpression vector and a construction method and application thereof, and relates to the technical field of livestock molecular biology. The porcine circular RNA is formed by cyclizing 3rd to 6th exons of an LDHC gene, and the nucleotide sequence of the porcine circular RNA is as shown in SEQ ID NO: 1. The porcine circLDHC is obtained by carrying out reverse transcription on porcine circular RNA (Ribonucleic Acid). According to the invention, a specific amplification primer is designed for circLDHC, and the real existence of circLDHC is verified through PCR (Polymerase Chain Reaction) amplification, sequencing and RNase R enzyme digestion. Fluorescent quantitative PCR analysis finds that circLDHC is highly expressed in sexual mature testis tissues of pigs, the expression level of circLDHC is related to testis maturation, and meanwhile, the expression quantity of circLDHC in testis of 75-day-old Meishan boars is remarkably higher than that of circLDHC in testis of same-day-old Duroc boars (plt; 0.01) of the substrate. The invention further finds that the over-expressed circLDHC inhibits the proliferation of the porcine sertoli cells, promotes the maturation of the porcine sertoli cells, lays a foundation for further researching the start of the puberty period of the boars, and provides a new thought for analyzing the sexual precocious puberty mechanism of the boars and improving the reproductive performance.

The invention provides a method for culturing primary spermatogonial stem cells of Tibetan sheep, and belongs to the technical field of cell separation and culture. The culture method provided by the invention comprises the following steps: disinfecting and cleaning testis tissues of Tibetan sheep lambs; shearing tissue blocks, and digesting and cleaning to obtain a single-cell suspension of the seminiferous tubule; purifying the single-cell suspension, and culturing without a feeding layer or with the feeding layer; the culture without the feeding layer refers to culture in a laminin-coated culture container by using a feeding-layer-free MEM alpha culture medium or a StemProTM-34SFM complete culture medium; culture with a feeding layer refers to culture in a mouse fibroblast line feeding layer or a Tibetan sheep primary testicular sertoli feeding layer, and a feeding layer MEMS alpha culture medium is added. According to the culture method provided by the invention, the Tibetan sheep spermatogonial stem cells with higher purity can be obtained through separation, the subsequent proliferation of the spermatogonial stem cells can be remarkably promoted, and the apoptosis of the spermatogonial stem cells can be inhibited.

The invention discloses an experimental device and method for simulating rat reproduction influence under exposure of a low-pressure anoxic environment, relates to the technical field of reproduction influence experiments, and provides the following scheme that the experimental device comprises a low-pressure experimental module fixed on the ground and a vacuum pump erected on the top surface of the low-pressure experimental module, the extraction end of the vacuum pump penetrates into an inner cavity of the low-pressure experiment module through a pipeline, a hatch is formed in the left side of the low-pressure experiment module, and a hatch door is installed on the end face of the hatch through a hinge. According to the technical scheme, by simulating the day and night temperature difference at the altitude of 4000 m and 6000 m and 12 DEG C / 2 DEG C in the low-pressure experiment module and simulating the plateau environment to feed rats, the plateau low-oxygen exposure environment can be closer to the plateau low-oxygen exposure environment, and by conducting slice detection on testicular tissue of the test rats, damage data of the low-pressure low-oxygen exposure environment to the male reproductive system is obtained. Therefore, the method has certain good reproducibility on reproductive system injury in a low-pressure and low-oxygen exposure environment.

The invention discloses application of mangiferin in preparation of a protective agent for testicular sertoli cells and preparation of a medicine for treating male infertility diseases, and relates to the technical field of medicine application. Wherein the mangiferin can play a role in protecting the sertoli cells, and the protection role is reflected in ways of improving the cell viability of the sertoli cells, inhibiting the apoptosis of the sertoli cells, inhibiting the oxidative stress of the sertoli cells, inhibiting the inflammatory response of the sertoli cells and the like; meanwhile, mangiferin can be used for treating male infertility diseases in ways of relieving weight loss, repairing testis, improving endocrine system functions, inhibiting oxidative stress of testis tissues, inhibiting inflammatory response of the testis tissues and the like. Wherein the mangiferin can inhibit the inflammatory reaction mainly by inhibiting the activation of NLRP3 inflammasome to inhibit the NLRP3 pathway, so that the inflammation and even pyroptosis of testicular sertoli cells are remarkably reduced to improve spermatogenesis, and the male infertility disease is well treated.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention relates to application of SMAD6 in auxiliary diagnosis and prognosis evaluation of TGCT (Transforming Growth Computed Tomography). According to the invention, a novel TGCT prognosis biomarker (SMAD6) is identified through difference analysis, Cox regression and survival analysis on the basis of a TGCT sample in multiple centers. Subsequently, the expression level of SMAD6 in normal testicular tissues and TGCT is evaluated through immunohistochemical staining. Finally, the relationship between the SMAD6 and biological characteristics, mutation landscape, immune cell infiltration and immunotherapy response is also studied. The invention defines that SMAD6 is a risk factor of poor TGCT prognosis. An immunohistochemical result shows that the SMAD6 is highly expressed in the TGCT tissue. GSEA shows that SMAD6 is related to activation of tumor-related pathways and inhibition of immune-related pathways. In addition, high SMAD6 expression is associated with lower CD8 + T cell infiltration, while patients with low SMAD6 expression gain more benefits in immunotherapy. The invention fully reveals the potential of SMAD6 as a TGCT prognosis and immunotherapy reaction biomarker, and provides a new scientific basis and theoretical basis for personalized treatment of TGCT.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The invention relates to the technical field of spermatogonial stem cell separation, in particular to a Tibetan sheep primary spermatogonial stem cell separation, purification and culture method and application thereof. The method comprises the following steps: (1) treating testicular tissues of Tibetan sheep by using mixed enzyme liquid and enzymatic hydrolysate in sequence to obtain single-cell suspension of seminiferous tubules; (2) purifying the single-cell suspension of the seminiferous tubules obtained in the step (1) by using a differential adhesion method to obtain spermatogonial stem cells; and (3) inoculating the spermatogonial stem cells obtained in the step (2) into a feeding layer for culture. The method is simple and easy to implement, the primary spermatogonial stem cells of the Tibetan sheep can be obtained through effective separation and purification, and the purity of the spermatogonial stem cells is high.

The invention discloses application of semen cuscutae-fructus lycii in preparation of a medicine for regulating and controlling an SIRT1 / Nrf2 signal channel to improve oligoasthenospermia, and belongs to the technical field of biology. According to the application of the semen cuscutae-fructus lycii in preparing the medicine for regulating and controlling the SIRT1 / Nrf2 signal channel to improve the oligoasthenospermia, disclosed by the invention, the semen cuscutae-fructus lycii regulates and controls the SIRT1 / Nrf2 expression of testicular tissues, enhances the anti-oxidative stress capability, up-regulates the BTB related protein expression level and repairs BTB damage, so that the spermatogenic function of OAS rats is improved, and the effect of improving the oligoasthenospermia is achieved. And the total flavonoids can be a material basis for playing a drug treatment effect. A powerful experimental support is provided for research on a mechanism of treating OAS by exerting the anti-oxidative stress effect of the semen cuscutae-fructus lycii, and a theoretical basis is provided for research on Ningxia genuine medicinal materials.

The present disclosure provides an iterative process for identifying culture conditions that maintain identity, growth, and survival of testicular cells in vitro. Testicular cell culturing systems for supporting human spermatogenesis and culture using identified culture conditions are also provided. The methods and the culture conditions can be used to culture healthy and viable spermatozoa with lower rates of deleterious or de novo mutations or epigenetic perturbations from fertile and infertile men for future use with assisted reproductive technologies.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

Disclosed herein are methods of reducing pathological inflammation and effects thereof through administration of tissues associated with regenerative and / or tolerogenic activity. In some embodiments, such tissues can include placental, ocular, testicular, and osseous tissues. Augmentation of therapeutic effects of said tissues may be achieved through contact with Injury Mimetics.

The invention belongs to the technical field of medicines, and particularly discloses application of afzelin in preparation of an anti-testis aging medicament, and testis aging resistance is particularly improvement or recovery of an aged testis tissue structure. The invention also provides a medicament for resisting testis aging. The medicament contains afzelin. The invention provides a new application of afzelin, the afzelin can be used for saving senescence testicular tissue structure disorder, remarkably reducing the epididymal sperm deformation rate and increasing the number of supporting cells and undifferentiated spermatogonium cells, the new application value of afzelin is developed, and a theoretical basis is provided for developing afzelin into anti-reproductive aging drugs.

The utility model provides a portable testicular tissue sampling and freezing device, and belongs to the field of medical instruments. The sampling and freezing device comprises a sampling component, a freezing component and a freezing component, wherein the sampling component is used for sampling testicular tissues to be treated; the processing assembly is used for washing and fixing the testicular tissues sampled by the sampling assembly; the cooling assembly is used for cooling the testicular tissue treated by the treatment assembly; the freezing assembly is used for cryopreserving the testicular tissue cooled by the cooling assembly, and the sampling assembly, the processing assembly, the cooling assembly and the freezing assembly are all stored in a portable transfer box. According to the portable testicular tissue sampling and freezing device, the problem of emergency fertility preservation of soldiers can be solved, the device is portable, testicular tissue samples of rescued objects can be rapidly collected in time, and the testicular tissues can be rapidly frozen and stored for fertility preservation.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The invention discloses a testis Leydig cell senescence marker and application thereof, the senescence marker is CCN5 protein, the senescence condition of the Leydig cell can be specifically reflected by detecting the CCN5 level of testis tissue, the senescence marker can be used as an index for evaluating Leydig cell senescence and testis senescence, and meanwhile, the senescence marker is an effective supplement for a diagnostic scheme of late onset hypogonadism (LOH). Furthermore, the invention also provides application of a preparation for inhibiting CCN5 protein expression in preparation of a product for improving testis Leydig cell senescence or treating diseases caused by testis Leydig cell senescence, the testis Leydig cell senescence can be improved and the LOH symptom can be treated by reducing CCN5 in a targeted manner, and treatment can be performed aiming at the core phenotypes of'lipid droplet loss' and'fibrosis' of testis Leydig cell senescence. The method is a treatment scheme different from exogenous testosterone supplement, stem cell injection, Senlytics drugs and exosome therapy.

The invention discloses a preparation method of a novel testicular long sperm tail marker detection kit, belongs to the technical field of biological medicines, and relates to a method for detecting testicular long sperm tails by using specific expression Vha68-3 protein in testis tissues. The kit is used for detecting the testis long sperm tail expression mode by detecting the expression condition of the 18-33rd amino acid ASNEDARNSSGSLNQD-C of the Vha68-3 protein in the testis tissue of the drosophila melanogaster. By detecting the expression mode of the Vha68-3 protein in the testis tissue, the differentiation condition of the extension process of the long testis sperms can be effectively judged, the kit prepared from the Vha68-3 protein can be effectively applied to detection of the expression distribution condition of the tails of the long testis sperms, and the Vha68-3 protein has important application value in research of the extension process of the long testis sperms.

An embodiment relates to polydeoxyribonucleotide (PDRN)-containing exosomes, a composition including same, and a preparation method therefor, wherein the PDRN-containing exosomes of the embodiment are derived from a fish testis tissue and include high-purity, low-molecular weight PDRN. In addition, exosomes containing a significant amount of low molecular weight PDRN can be induced through a simple process of ultrasonic treatment. Furthermore, application of ultrasound to the separated exosomes can prepare PDRN exosomes that are made to have low molecular weights, whereby the exosomes exhibit high intracellular uptake rates and thus can be advantageously used for manufacturing a tissue regeneration or anti-inflammatory composition.

The invention discloses a method for rapidly extracting protamine sulfate, which comprises the following steps: S1, mixing testis tissue and a sodium chloride solution, pulping, standing, centrifuging, and filtering to obtain a precipitate; s2, mixing the precipitate with a sulfuric acid solution, carrying out ultrasonic extraction by using at least two ultrasonic waves with different frequencies at the same time, and centrifuging and filtering to obtain an extracting solution; and S3, carrying out alcohol precipitation on the extracted mixed solution, and drying to obtain protamine sulfate. In the process of extracting protamine by using sulfuric acid, a plurality of ultrasonic waves with different frequencies are adopted to carry out ultrasonic treatment at the same time to generate more cavitation effects and mechanical effects, so that more protamine is dissolved out, and the extraction rate of protamine sulfate is improved.

The invention discloses an application of Atg8b in a sperm release disorder diagnostic kit, the kit takes low-expression Atg8b in testis tissue as a marker of sperm release disorder NOA, and the kit uses Atg8b mRNA to detect the expression level of Atg8b in testis sample tissue. The expression level of Atg8b in the testicular tissue is detected to effectively judge whether the expression of the sperm release disorder NOA exists or not, the expression level of Atg8b in the testicular tissue with the sperm release disorder is remarkably reduced, the Atg8b serves as a key protein, testicular end epithelial cells are mainly regulated and controlled through a cell adhesion mechanism, the sperm release process is mediated, and the sperm release disorder NOA can be effectively detected. Finally, the sperm release disorder type NOA drosophila melanogaster is formed, and Atg8b is an important diagnostic marker for sperm release disorder and has good clinical diagnostic value in a sperm release disorder diagnostic kit.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

Provided in the present invention is a culture solution for promoting in-vitro human spermatogenesis and the use thereof. The culture solution comprises the following components in volume fraction: 80%-95% of αMEM, and 5%-20% of a serum substitute and 50-100 ng / mL transforming growth factor β family activin A, 10-50 ng / mL stem cell growth factor, 10-50 ng / mL bone morphogenetic protein, 10-7-10-5 M retinoic acid, 10-50 mM testosterone, 100-200 ng / mL follicle-stimulating hormone, and 50-100 mg / mL pituitary extract. Testicular tissue with meiotic arrest of human germ cells is separated into small pieces, and same are cultured for 10-30 days in the culture solution for promoting in-vitro human spermatogenesis, such that human spermatids can be obtained. The culture solution of the present invention promotes the in-vitro maturation of human male germ cells, which provides a basis for research on human spermatogenesis, and provides technical support for the clinical treatment of male infertility.

The invention belongs to the technical field of reproductive biology, and particularly provides a method for separating and culturing testicular perichondrial muscle-like cells of an adult mouse. The method comprises the following steps: removing interstitial cells from testis tissues of an adult mouse, and digesting the testis tissues by pancreatin and DNA enzyme to obtain a testis single-cell suspension; and culturing by adopting a new isolated culture medium until the culture dish is full of cells, and carrying out cell passage, cell cryopreservation and resuscitation and cell identification by adopting passage culture mediums with different concentrations. According to the method for separating and culturing the mouse peridermoid cells, the cell separation degree is far higher than that in the prior art, operation is easy, time is saved, the technical blank that the mouse peridermoid cells are difficult to separate and culture is filled, and the method is suitable for large-scale popularization and application. And convenience is provided for research on interaction of the mouse testicular peritubular muscle-like cells with other testicular somatic cells and germ cells.

The invention discloses application of a xanthoceras sorbifolia extract in preparation of a medicine for preventing and / or treating electromagnetic radiation damage. Experiments show that the xanthoceras sorbifolia bunge flower extract can improve the weight loss of rats caused by microwave radiation; brain tissue structure change damage caused by microwave radiation is improved; the reduction of the learning and memory ability of the rats caused by microwave radiation is improved; the brain sea horse tissue amino acid neurotransmitter disorder caused by microwave radiation is improved; electroencephalogram alpha and beta wave abnormity caused by microwave radiation is improved; the sperm motility reduction caused by microwave radiation is improved; the rat serum sex hormone disorder caused by microwave radiation is improved; rat testicular tissue oxidative stress injury caused by microwave radiation is improved; the abnormal energy metabolism of testicular tissues caused by microwave radiation is improved; heart electrophysiological abnormality and heart tissue ultrastructure abnormality caused by microwave radiation are improved; the immune function decline caused by microwave radiation is improved; the influence of microwave radiation on the content of IL-2, 4 is improved; the influence of microwave radiation on the thymus and spleen tissue structure is improved.