The invention provides a serum pancreatic cancer exosome specific protein, which comprises a protein ADAM10, a protein CFHR1, a protein APOA4, a protein testicular tissue protein Li 70, a protein HEL-S-78p, a protein PGLYRP2, a protein IGFALS, a protein BCHE, a protein ANPEP, a protein LBP, a protein C9, a protein PZP and a protein ANXA11. The invention further provides a re-verification process of the serum pancreatic cancer exosome specific protein, which comprises the steps of S1, separating the exosome-free serum and the exosome in a serum sample; S2, preparing an exosome PBS solution by adopting the exosome separated in the step S1; S3, checking the ultra-micro structure of the exosome through a transmission electron microscope; and S4, detecting the expression of some maker proteinson the surface of the exosome by through Western blot analysis. The serum pancreatic cancer exosome specific protein of the invention can be used for preparing related product for pancreatic cancer diagnosis, the re-verification process of the serum pancreatic cancer exosome specific protein has the advantages of absolute specificity, high throughput, no interference and ability of recognizing macromolecular isomers and small molecules.