59 results about "Testicular tissue" patented technology

The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.

The invention relates to the technical field of reproductive medicine, in particular to a high-quality sperm acquisition method based on a testis tissue sample of an azoospermia patient, which comprises the following steps: inputting a first frame of a testis tissue sample microscopic video sequence into a target detection model, outputting a sperm detection frame and confidence, and taking the position of the detection frame as a starting point of a motion trail; a dynamic confidence matching strategy is adopted, firstly, a prediction trajectory is matched with a high-confidence detection result, then, a real leak detection target is complemented with a low-confidence target, multi-stage Hungary matching is achieved through confidence guidance, and all sperm movement trajectories obtained in each frame are obtained; and based on multiple frames of images corresponding to all sperm movement tracks corresponding to the microscopic video sequence, selecting the multiple frames of images according to a preset number of confidence coefficients to construct a candidate morphological analysis sample set, and performing kinematics parameter calculation and morphological analysis to obtain high-quality target sperms. According to the method, the recognition efficiency of high-quality sperms is improved, the sperms with higher quality can be obtained, and the subsequent fertilization success rate is further improved.

The invention discloses application of mangiferin in preparation of a protective agent for testicular sertoli cells and preparation of a medicine for treating male infertility diseases, and relates to the technical field of medicine application. Wherein the mangiferin can play a role in protecting the sertoli cells, and the protection role is reflected in ways of improving the cell viability of the sertoli cells, inhibiting the apoptosis of the sertoli cells, inhibiting the oxidative stress of the sertoli cells, inhibiting the inflammatory response of the sertoli cells and the like; meanwhile, mangiferin can be used for treating male infertility diseases in ways of relieving weight loss, repairing testis, improving endocrine system functions, inhibiting oxidative stress of testis tissues, inhibiting inflammatory response of the testis tissues and the like. Wherein the mangiferin can inhibit the inflammatory reaction mainly by inhibiting the activation of NLRP3 inflammasome to inhibit the NLRP3 pathway, so that the inflammation and even pyroptosis of testicular sertoli cells are remarkably reduced to improve spermatogenesis, and the male infertility disease is well treated.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

This invention provides a biomarker for spermatogenesis disorders and its application, wherein the biomarker is the MNDA molecule. This application characterizes the functional abnormalities or spermatogenesis disorders of Sertoli cells by detecting the expression level of MNDA molecules on cells in testicular tissue sections, and can therefore be used for the clinical diagnosis of non-obstructive azoospermia.

Disclosed herein are methods of reducing pathological inflammation and effects thereof through administration of tissues associated with regenerative and / or tolerogenic activity. In some embodiments, such tissues can include placental, ocular, testicular, and osseous tissues. Augmentation of therapeutic effects of said tissues may be achieved through contact with Injury Mimetics.

The invention belongs to the technical field of medicines, and particularly discloses application of afzelin in preparation of an anti-testis aging medicament, and testis aging resistance is particularly improvement or recovery of an aged testis tissue structure. The invention also provides a medicament for resisting testis aging. The medicament contains afzelin. The invention provides a new application of afzelin, the afzelin can be used for saving senescence testicular tissue structure disorder, remarkably reducing the epididymal sperm deformation rate and increasing the number of supporting cells and undifferentiated spermatogonium cells, the new application value of afzelin is developed, and a theoretical basis is provided for developing afzelin into anti-reproductive aging drugs.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

An embodiment relates to polydeoxyribonucleotide (PDRN)-containing exosomes, a composition including same, and a preparation method therefor, wherein the PDRN-containing exosomes of the embodiment are derived from a fish testis tissue and include high-purity, low-molecular weight PDRN. In addition, exosomes containing a significant amount of low molecular weight PDRN can be induced through a simple process of ultrasonic treatment. Furthermore, application of ultrasound to the separated exosomes can prepare PDRN exosomes that are made to have low molecular weights, whereby the exosomes exhibit high intracellular uptake rates and thus can be advantageously used for manufacturing a tissue regeneration or anti-inflammatory composition.

The invention discloses an application of Atg8b in a sperm release disorder diagnostic kit, the kit takes low-expression Atg8b in testis tissue as a marker of sperm release disorder NOA, and the kit uses Atg8b mRNA to detect the expression level of Atg8b in testis sample tissue. The expression level of Atg8b in the testicular tissue is detected to effectively judge whether the expression of the sperm release disorder NOA exists or not, the expression level of Atg8b in the testicular tissue with the sperm release disorder is remarkably reduced, the Atg8b serves as a key protein, testicular end epithelial cells are mainly regulated and controlled through a cell adhesion mechanism, the sperm release process is mediated, and the sperm release disorder NOA can be effectively detected. Finally, the sperm release disorder type NOA drosophila melanogaster is formed, and Atg8b is an important diagnostic marker for sperm release disorder and has good clinical diagnostic value in a sperm release disorder diagnostic kit.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The invention belongs to the technical field of reproductive biology, and particularly provides a method for separating and culturing testicular perichondrial muscle-like cells of an adult mouse. The method comprises the following steps: removing interstitial cells from testis tissues of an adult mouse, and digesting the testis tissues by pancreatin and DNA enzyme to obtain a testis single-cell suspension; and culturing by adopting a new isolated culture medium until the culture dish is full of cells, and carrying out cell passage, cell cryopreservation and resuscitation and cell identification by adopting passage culture mediums with different concentrations. According to the method for separating and culturing the mouse peridermoid cells, the cell separation degree is far higher than that in the prior art, operation is easy, time is saved, the technical blank that the mouse peridermoid cells are difficult to separate and culture is filled, and the method is suitable for large-scale popularization and application. And convenience is provided for research on interaction of the mouse testicular peritubular muscle-like cells with other testicular somatic cells and germ cells.

The invention discloses application of a xanthoceras sorbifolia extract in preparation of a medicine for preventing and / or treating electromagnetic radiation damage. Experiments show that the xanthoceras sorbifolia bunge flower extract can improve the weight loss of rats caused by microwave radiation; brain tissue structure change damage caused by microwave radiation is improved; the reduction of the learning and memory ability of the rats caused by microwave radiation is improved; the brain sea horse tissue amino acid neurotransmitter disorder caused by microwave radiation is improved; electroencephalogram alpha and beta wave abnormity caused by microwave radiation is improved; the sperm motility reduction caused by microwave radiation is improved; the rat serum sex hormone disorder caused by microwave radiation is improved; rat testicular tissue oxidative stress injury caused by microwave radiation is improved; the abnormal energy metabolism of testicular tissues caused by microwave radiation is improved; heart electrophysiological abnormality and heart tissue ultrastructure abnormality caused by microwave radiation are improved; the immune function decline caused by microwave radiation is improved; the influence of microwave radiation on the content of IL-2, 4 is improved; the influence of microwave radiation on the thymus and spleen tissue structure is improved.

The invention provides a gene influencing sexual precocity of deep-county pigs and a screening method thereof, and belongs to the field of research on sexual maturity of pigs, the gene influencing sexual precocity of deep-county pigs comprises an SRD5A1 gene and / or a CYP11B2 gene, testicular tissues of deep-county pigs before and after sexual maturity are subjected to transcriptome sequencing, differential expression gene functions are analyzed, and the sexual precocity of deep-county pigs before and after sexual maturity is obtained. And screening out candidate genes related to the sexual maturity of the deep county pigs. And then performing transcriptome sequencing on testis tissues of the sexually mature deep county pig and the hybrid pig of the sexually mature deep county pig and the large white pig, analyzing functions of differential expression genes, and screening specific expression genes of the sexual maturity of the deep county pig. Integrating the results of the two times of transcriptome sequencing, analyzing the functions of the coincident genes, and screening candidate genes SRD5A1 and CYP11B2 which influence the sexual precocity of the pigs in the deep county according to the gene functions; and a foundation is laid for subsequent research on precocious puberty of pigs in deep county.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The invention relates to the technical field of poultry reproductive biology, and particularly discloses a phosphorylated protein marker for fasting-induced chicken blood testis barrier remodeling, a screening method and application. The marker comprises a Ser1573 phosphorylation site which is tightly connected with the protein ZO-1 and a Ser1620 phosphorylation site of the ZO-1. The screening method comprises the following steps: collecting testicular tissues of cocks at key time points of fasting and recovering foraging, carrying out proteomics and phosphorylation detection, analyzing and screening different phosphorylation sites in combination with bioinformatics, and carrying out multi-dimensional verification through Western blot, immunofluorescence and cell function experiments. The invention provides a key molecular marker for evaluating the testis functional state of the cock and optimizing a limited feeding scheme in breeding of the breeder cock, and has important production and application values.

This invention relates to the application of SMAD6 in the auxiliary diagnosis and prognostic assessment of TGCT. Based on TGCT samples, this invention identifies a novel TGCT prognostic biomarker (SMAD6) through differential analysis, Cox regression, and survival analysis. Subsequently, the expression level of SMAD6 in normal testicular tissue and TGCT was assessed by immunohistochemical staining. Finally, the relationship between SMAD6 and biological characteristics, mutant landscape, immune cell infiltration, and immunotherapy response was investigated. This invention clarifies that SMAD6 is a risk factor for poor prognosis in TGCT. Immunohistochemical results show that SMAD6 is highly expressed in TGCT tissues. GSEA indicates that SMAD6 is associated with the activation of tumor-related pathways and the inhibition of immune-related pathways. Furthermore, high SMAD6 expression is associated with lower CD8 levels. + T-cell infiltration is associated with low SMAD6 expression, and patients with low SMAD6 expression benefit more from immunotherapy.

The invention discloses an application of curcumin in improving puberty male reproductive capacity damage caused by chemotherapeutic drugs. Aiming at the problems of testicular germ cell quantity reduction, oxidative stress injury, long-term fertility damage and the like caused by cytosine arabinoside chemotherapy, curcumin is applied while or before the cytosine arabinoside is applied. The application is realized through combined administration or a pharmaceutical composition containing curcumin and cytarabine. Experiments show that the scheme can effectively relieve testicular tissue form damage, reduce germ cell apoptosis and antagonize oxidative stress, and significantly improve the sperm quality of adult individuals. The invention provides a new potential strategy for synchronously protecting the reproductive function of a clinical child tumor patient during chemotherapy.

The invention relates to the technical field of medicines, and discloses an application of lupin or an extract thereof in preparation of a drug for relieving reproductive toxicity of chemotherapeutic drugs, the lupin is selected from lupin plants, preferably lupin variety Jenabillup, and the chemotherapeutic drugs comprise busulfan, cyclophosphamide or cis-platinum. The administration dosage of the lupin or the extract thereof is 0.1-100 mg / kg body weight, the administration period is continuous administration for at least 5 weeks, experiments show that the lupin can significantly improve the sperm concentration and sperm motility of chemotherapy model animals and improve pathological damage of testicular tissues, the mechanism relates to antioxidant, anti-inflammatory and anti-apoptosis effects, and the lupin or the extract thereof can be used for preparing a chemotherapy model animal. The invention provides a safe and effective new strategy for fertility protection of chemotherapy patients.

The invention discloses a method for improving inhibition of slow heat stress on male rabbit breeding, which is executed by a computer system and comprises the following steps: acquiring environmental data and physiological indexes such as male rabbit ear temperature and respiratory rate in real time through a multi-modal sensor; the core algorithm constructs a slow heat stress cumulative damage model containing a time decay factor, and quantifies the integral effect of the heat load and the body repair process. The system generates multi-dimensional optimal control vectors of environment cooling, nutrition intervention and LED red light repair through a physiology-environment coordinated regulation and control model on the basis of an accumulated damage index generated by the model. According to the method, the limitation of traditional single threshold value control is broken through, and full-period precise prevention and control of physical cooling open circuit, nutrition restoration follow-up and illumination rehabilitation ending is achieved. Experiments prove that the scheme can effectively protect testis tissues, shorten the reproductive function recovery period and improve the semen quality, the breeding and medicament cost is reduced while the reproductive performance is guaranteed, and the method is suitable for different scales of rabbit farms.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to sperm-producing decoction as well as a preparation method, application and a product thereof. The sperm-producing decoction is prepared from the following raw materials in parts by weight: 5 to 15 parts of radix salviae miltiorrhizae, 5 to 15 parts of rhizoma chuanxiong, 5 to 15 parts of radix angelicae sinensis, 10 to 25 parts of herba epimedii, 10 to 25 parts of fructus cnidii, 10 to 25 parts of semen cuscutae, 5 to 15 parts of fructus rubi and 5 to 15 parts of fructus schisandrae chinensis. The spermatogenic decoction can improve testis injury caused by an anti-inflammatory and anti-immune drug tripterygium wilfordii hormone, improve oxidative injury of testis tissues, regulate the expression level of antioxidant stress related proteins Nrf2 and HO-1, improve the phenomena of testis diminishment, seminiferous tubule damage, sperm number reduction, sperm motility weakening, testis spermatogenic cell apoptosis and the like, and can be used for preparing the anti-inflammatory and anti-immune drug. And the expression level of apoptosis-related proteins Bcl-2 and Bax of the testicular spermatogenic cells is regulated.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The invention relates to the technical field of animal science and animal production, in particular to application of melatonin in preparation of a medicine for relieving cadmium-induced pig testicular tissue injury. Cadmium has testicular toxicity, which shows that cadmium exposure changes the transcriptional level of testicular genes, causes oxidative stress, activates inflammation and NF-kB signal channels, mediates abnormal iron metabolism, causes cell ferroptosis, and finally causes testicular tissue cell structure damage. It is found for the first time that an anti-oxidation defense system is remodeled to a certain extent through addition of melatonin, oxidative stress, inflammation and ferroptosis induced by cadmium exposure are inhibited, and testicular tissue cell structure damage is relieved. The invention provides a theoretical basis for relieving the damage of the environmental pollutant cadmium by using the melatonin in production practice.

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent for inhibiting PPAR alpha expression in preparation of a medicine for treating male infertility diseases, wherein a PPAR alpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the number of sperm in a Cd+GW6471 group is higher than that in a Cd+NAC group, the serum testosterone level in the Cd+GW6471 group is also higher than that in the Cd+NAC group, and the results of testicular tissue pathological staining also show that GW6471 can more significantly restore the decrease in the number of mature spermatogenic tubules caused by Cd, GW6471 restores the decrease in the number of SYCP3 positive spermatocytes caused by Cd, so that the sperm production efficiency is restored, and therefore the application of the reagent for inhibiting PPAR alpha expression in preparation of the medicine for treating male infertility diseases is proposed.

The invention belongs to the technical field of culture media, and discloses a culture medium for in-vitro culture of cynomolgus monkey testicular tissue. Through an innovative culture technology and in combination with a culture formula of adding ANGPTL4 and IGF1, the survival rate and spermatogenesis efficiency of testis tissues are optimized, an efficient and reliable technical means is provided for reproductive medicine research and fertility preservation, and the method is particularly suitable for pre-adolescent individuals or wild protection animals or humans needing to preserve fertility potential. The innovative rotary culture technology and the culture scheme that 20 ng / mL of ANGPTL4 and 20 ng / mL of IGF1 are additionally supplemented are beneficial to the integrity of testicular tissues in the in-vitro culture process and promote proliferation and differentiation of germ cells, and the spermatogenesis efficiency is improved. And a feasible technical scheme and a new insight can be provided for the research of male animal in-vitro spermatogenesis.

The invention relates to the technical field of animal reproductive biology, and discloses a single-cell multi-omics method for analyzing a cattle testis development and spermatogenesis regulation mechanism, which comprises the following steps: (a) acquiring testis tissue samples of cattle at different development stages; (b) respectively carrying out single cell RNA sequencing (scRNA-seq) and single cell nucleus ATAC sequencing (snATAC-seq) on the testicular tissue sample, so as to obtain transcriptome data and chromatin accessibility data. According to the single-cell multi-omics method for analyzing the regulation and control mechanism of testis development and spermatogenesis of the cattle, by integrating single-cell RNA sequencing and single-cell nucleus ATAC sequencing, nine main cell types and specific marker genes (including newly found marker genes such as CPEB1, ADGRG1 and the like) of testis of the Simmental cattle from birth to puberty are systematically identified; the composition change rule of each cell type in different development stages is finely described, and a comprehensive cell map is provided for understanding the development of the bovine testis.

The invention relates to application of ginsenoside Rg4 as a unique active ingredient in preparation of a medicine for improving male delayed gonad hypofunction, belonging to the field of biological medicine. According to the invention, the action mechanism of the Rg4 for improving LOH is defined for the first time: the Rg4 can specifically up-regulate the mRNA expression quantity of StAR and StARD7 in a testis tissue, and promote the transport of cholesterol to the inner mitochondrial membrane of a testis Leydig cell from the transcriptional level; the Rg4 improves the mRNA expression quantity of P450scc, 3 beta-HSD in testicular tissues by promoting the efficient transport of cholesterol, enhances the conversion of cholesterol to pregnenolone and the catalytic synthesis of subsequent steroids, and improves the endogenous testosterone synthesis capability; the Rg4 can recover the negative feedback regulation function of an HPG axis, regulate secretion of pituitary LH and FSH to physiological steady state, and avoid abnormal fluctuation of hormone level. The invention fills the technical blank of Rg4 in the field of LOH gene regulation and control.