128 results about "Animal Sources" patented technology

The invention relates to method for obtaining collagen from animal sources such as fresh or saltwater fish, chicken, eggs or from parts of these animal sources that can be considered waste, and to a collagen hydrolysate obtained as a result of this method.

Relating to the field of biotechnology, disclosed are a recombinant human elastin peptide with anti-aging effects and a use thereof. The recombinant human elastin peptide comprises an amino acid sequence shown in SEQ ID NO:1 or a fragment of the amino acid sequence shown in SEQ ID NO:1, the fragment comprises 30% or more of SEQ ID NO:1, or a mutant of the amino acid sequence shown in SEQ ID NO:1 or the fragment of the amino acid sequence shown in SEQ ID NO:1. A disclosed method for producing elastin peptides by using a yeast expression system enables the targeted synthesis of highly effective protein fragments with 100% homology to human proteins, while achieving low costs and facilitating large-scale production, avoiding animal-derived sources and viral risks. This makes the recombinant human elastin peptide safe for use as an anti-aging and anti-photoaging active ingredient in cosmetics, while also supporting a use thereof in the field of biomedical materials.

The invention discloses an animal source type I collagen liposome as well as a preparation method and application thereof. The method comprises the following steps: respectively dispersing two phospholipids and cholesterol in an organic solvent, carrying out rotary evaporation on the organic solvent, carrying out ultrasonic elution by using a buffer solution, homogenizing to form liposome balloons with uniform particle sizes, dialyzing by using the buffer solution, centrifuging to obtain liposome suspended matters, adding the suspended matters into an animal-derived I-type collagen solution with fixed concentration and pH, and carrying out freeze drying to obtain the collagen liposome. Stirring for a certain time at constant temperature, and centrifuging to obtain a final substance. The collagen coated with the lipidosome has a complete triple helix structure, and the D90 of the lipidosome is 680.51 nm. The liposome can be used for high-end cosmetics, such as essence, emulsion, cream, cream and other products.

The invention discloses synthesized XVII type humanized collagen as well as a preparation method and application thereof, and particularly relates to the technical field of genetic engineering. The amino acid sequence of the recombinant XVII type collagen is as shown in SEQ ID NO. 1. According to the invention, key functional domains (five high-hydrophilicity and high-stability peptide fragments such as 641-685 and the like) in a human XVII type collagen alpha1 chain are retained, and non-functional regions are removed. The 100% human source sequence coverage rate is adopted, the immunogenicity risk of animal source collagen is completely avoided, and the biocompatibility is excellent. The functional peptide fragment is repeated twice to form a stable structure of 210 amino acids, so that the functional activity of the protein is enhanced. The theoretical isoelectric point (pI = 6.07) is close to the pH value (7.4) of human tissue fluid, the dissolvability is excellent, and the applicability is high.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.

A method of decellularization of animal tissue, comprising the steps of: mechanical homogenization of animal-derived tissue, incubation of the homogenized tissue from step a) in a solution of trypsin and EDTA, subjecting the filtered tissue to incubation in a PBS buffer solution with a pH in the range of 7.2-7.6 containing a non-ionic detergent at a concentration of 4 - 2% and NH4OH at a concentration of 0.2 - 0.08%, rinsing the tissue with a PBS buffer solution containing an antimicrobial agent, incubation in a deoxyribonuclease solution at a concentration of 0.001 to 0.003% (w / v), rinsing the decellularized tissues obtained with a PBS buffer solution containing an antimicrobial agent, wherein the non-ionic detergent is a compound with a molecular weight of 596 g / mol and additional steps of incubating the tissue in the solution PBS buffer with a pH of 7.2- 7.6 containing a non-ionic detergent at a concentration of 1.5-0.5% and NH4OH ata concentration of 0.2-0.08%, where the incubation is carried out at a temperature below room temperature for 36-48h, and incubation of the tissue in a PBS buffer solution with a pH of 7.2-7.6 containing a non- ionic detergent at a concentration of 0.75-0.25% and NH4OH at a concentration of 0.2-0.08%, where the incubation is carried out at a temperature below room temperature for 36-48h. Bioink containing decellularized tissue of animal origin.

The invention discloses a cryopreservation solution suitable for fibroblasts and a preparation method and application thereof, and relates to the technical field of cell cryopreservation solutions, the cryopreservation solution suitable for fibroblasts is prepared from the following components: a cryoprotectant, a cell nutrient, an antioxidant and a solvent, and does not contain animal-derived serum or dimethyl sulfoxide; the cryoprotectant comprises a propylene glycol solution and an ethylene glycol solution, trehalose is adopted as the cell nutrient; glutathione is adopted as the antioxidant; the volume ratio of the propylene glycol solution to the ethylene glycol solution to the trehalose to the glutathione to the compound electrolyte is 10: 10: 5: 1: 74; the fibroblast cryopreservation liquid is simple in component, free of DSMO and animal-derived serum, simple and convenient in preparation process, long in validity period, convenient in storage condition, free of programmed cooling and suitable for cryopreservation of fibroblasts; the recovery rate of the cryopreserved living cells is higher than 75.0%, the average recovery rate is up to 85%, the proliferation capacity of the cryopreserved and recovered cells is high, and the cell activity is kept stable.

The present invention relates to the field of biotechnology and discloses a recombinant human elastin peptide with anti-aging efficacy and its application in a composition thereof. The recombinant human elastin peptide comprises the elastin peptide amino acid sequence SEQ ID: 1 or a fragment thereof, wherein the fragment comprises 30% of SEQ ID: 1, or a fragment comprises more than 30% of SEQ ID: 1, or a mutant of the above sequence. The recombinant human elastin peptide can be directed to synthesize a protein of a highly effective fragment, with a 100% human homologous sequence, while being low-cost, easy to mass-produce, avoiding animal sources, and avoiding viral risks. This allows the recombinant human elastin peptide to be safely used in cosmetics as an anti-aging and anti-photoaging ingredient, while also providing support for its application in the field of biomedical materials.

To provide a method for producing a decellularized tissue or organ that enables short-term completion of decellularization of tissue or organ while ensuring superior structural retention in the produced decellularized tissue or organ.SOLUTION: Disclosed is a method for producing a decellularized tissue or organ, the method comprising: a step for incorporating a DNA-degrading enzyme into an animal-derived tissue or organ; and a step for applying a hydrostatic pressure of 100-500 MPa in a medium that contains the DNA-degrading enzyme to the animal-derived tissue or organ into which the DNA-degrading enzyme has been incorporated.SELECTED DRAWING: Figure 2

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

A foodstuff comprises animal-derived meat and a filamentous fungus, for example Fusarium venenatum. It may comprise: 3.0 to 25.0 wt% animal-derived meat on a dry mass basis; 2.0 to 25.0 wt% fungus on a dry mass basis; 50.0 to 90.0 wt% water; 0.0 to 6.0 wt% flavourant(s); and 0.0 to 15.0 wt% binder(s).

The invention relates to the technical field of medicines, in particular to novel application of galactooligosaccharide. The novel use of galactooligosaccharide inhibits the formation of gallstone. Main reagents and materials are galactooligosaccharide; a mouse calculus model feed; the experimental animals are derived from animal varieties and strains: mice, C57BL / 6J; sex: male; week age: 4 week age; the body weight is 16-20 g; the experimental environment is SPF environment feeding, and the feeding condition is that the room temperature is 20-26 DEG C; the relative humidity is 40-70%; automatic illumination is achieved, and light and shade are alternated for 12 h; the drinking water is RO membrane reverse osmosis sterile water; the feeding density is defaulted to be 4 per cage, and the padding is replaced once a week; the experimental process comprises the following steps: starting an experiment after experimental feeding of all mice for one week, and randomly dividing 36 5-week male C57BL / 6J mice into three groups, namely 12 mice in each group. The invention provides a new application of galactooligosaccharide, which can inhibit the formation of gallstone and provides a new thought for preventing the occurrence of the gallstone.

The invention relates to the technical field of bacterial culture, particularly discloses a culture method free of animal source components and suitable for Akk growth, and aims to solve the problem that viruses, toxins, allergens and the like are possibly carried in the culture process in the prior art. Obtaining Akk in a frozen state, and unfreezing at room temperature; inoculating into a target culture medium according to the inoculum size of 10%, standing and culturing for 48 hours in a preset air environment, and activating three generations to restore the vitality of thalli; the target culture medium comprises the following components: 11.38 g / L of yeast extract powder, 34.13 g / L of neutral soy peptone, 3.5 g / L of glucose, 4.2 g / L of disodium hydrogen phosphate, 3 g / L of galactooligosaccharide, 3 g / L of N-acetyl glucosamine, 1.5 g / L of threonine and 0.5 g / L of L-cysteine. The culture medium provided by the invention is suitable for Akk growth, has simple components, does not contain animal-derived components, does not carry viruses, toxins, allergens and the like, and meets the requirements of people.

The present invention relates generally to devices and methods for reducing the water content of inorganic and / or organic products or compounds of plant or animal origin, in particular to devices and methods for removing a majority of water present in products or compounds of plant or animal origin, the present invention relates to a method for preparing inorganic compounds or products to be used in other processes or to obtain finished products, such that it can be preserved (even over a long period of time) without undesirable fermentation and significant modifications in nutritional and organoleptic properties of organic compounds, and to prepare inorganic compounds or products to be used in other processes or to obtain finished products.

The application discloses a freeze-drying protective agent for Streptococcus pneumoniae without animal source, and belongs to the field of microorganism preservation. The freeze-drying protective agent is composed of the following components: 1-5% (weight / volume) soybean peptone, 1-5% (weight / volume) trehalose, 4-8% (weight / volume) mannitol and 0.3-1.5% (volume / volume) glycerol, and the rest is water. The freeze-drying protective agent can better maintain the activity of Streptococcus pneumoniae, and the effect is equivalent to that of a skimmed milk protective agent or a commercially available freeze-drying protective agent without animal source, but the freeze-drying protective agent does not contain animal source components and has low cost.

Compositions comprising oils of plant or animal origin, such as fatty acid alkyl esters, are stabilized against oxidation using oxidized aminophenol antioxidants. The aminophenol includes one or two carbon-containing groups including hydroxyl groups and / or ether groups separated from an N atom by one or more carbon atoms. The oxidized aminophenol antioxidants effectively control oxidation in moderate and even low amounts, can be used in a variety of different oil and fatty acid methyl ester formulations, and are effective at elevated temperatures, and exhibit significantly superior performance to conventional antioxidants.

The present invention relates to a method for obtaining a fertiliser by pyrolysis of raw materials of animal origin, in particular selected from hair, feathers, wool, or meat meal. The product obtained by said pyrolysis can be used directly as a fertiliser or as a raw material for obtaining fertilisers.

A pharmaceutical composition comprising: an active pharmaceutical ingredient (API); 1-45 wt% non-gelatin matrix former comprising hydroxypropyl methylcellulose (hypromellose, HPMC), methyl cellulose,

The present invention provides a textured plant protein defined as a fibrous plant protein material having a protein content of at least 40 wt.% relative to dry matter wherein the textured plant protein is prepared by extruding a feed composition at a moisture content of 18.1-25.9 wt.%, the feed composition comprises: a) a source of plant protein from seeds, cereals, algae, fruits, leaves or legumes, comprising at least 65 wt.% of plant protein relative to dry matter; and b) a source of tuber protein comprising at least 75 wt.% of tuber protein relative to dry matter, and c) a filler, In addition, the invention provides food products comprising the TVP of the invention, as well as methods for obtaining these products, and the use of TVP in food products to replace meat of animal origin.

The present invention relates to an ACCS gene, a protein and the use thereof. An involved ACCS protein mutant is formed by means of mutating a proline residue at position 134 and a tryptophan residue at position 136 of an animal-derived wild-type ACCS protein into a glutamine residue and a tyrosine residue, respectively. The involved ACCS protein mutant can be used in the intervention and treatment of a variety of diseases comprising brain diseases, and provides a new regimen and approach for the intervention and treatment of brain diseases.

The invention relates to the technical field of hydrogel, in particular to diseased animal source amino acid nutritional hydrogel for smart agriculture and a preparation method thereof.The diseased animal source amino acid serves as a nutritional ingredient, and the diseased animal source amino acid is stably integrated into a hydrogel network through photo-initiation through modification treatment; the preparation method comprises the following steps: by taking multi-aldehyde hemp cellulose as a carrier, grafting with compound amino acid through Schiff base reaction to obtain grafted compound amino acid; then taking the grafted compound amino acid as a core material and starch octenylsuccinate as a wall material to prepare a compound amino acid microcapsule; one of polyvinyl alcohol, polyethylene glycol, polyacrylamide and polyvinylpyrrolidone is used as an additive to be compounded with a conventional fertilizer; arabic gum, carboxymethyl chitosan and sodium alginate are compounded to serve as a degradable hydrogel matrix, acrylic acid is grafted to a polymer network, compound amino acid microcapsules with double bonds on the surfaces serve as nutritional ingredients, and the hydrogel is prepared through light-induced polymerization.

This invention relates to a system of generation of ammonia from urea. Urea reacts to urease to generate ammonia. The ammonia generated could be cooled to liquified and collected in containers. The reaction chamber is heated about 60 degrees Celsius to 80 degrees Celsius. The urine and urease extracted from legumes like soyabean are added and stirred for well mixing. The ammonia generated would pass through the filter system for air. Then it would dehumidify to dry. The ammonia is either liquified or directly passed to the next chamber as gaseous form for production of urea.

The invention belongs to the technical field of cell biology, and particularly relates to a method for amplifying clinical-grade hair follicle stem cells on a large scale and application of the clinical-grade hair follicle stem cells in preparation of products for treating alopecia. According to the method provided by the invention, stable amplification of the hair follicle stem cells can be realized by accurately controlling passage times, and reagents used in the culture process do not contain animal sources, so that the possibility of animal source pathogen pollution does not exist. The clinical-grade hair follicle stem cells amplified in a large scale by the method provided by the invention can provide high-quality cell resources for alopecia patients, can be used for hair follicle regeneration treatment, and can be transplanted to an alopecia area to stimulate hair follicle regeneration so as to realize regeneration and growth of hair.

A method and compositions of treatment for improving skin health and sleep quality using supplementation, comprising the step of applying a supplement formulation to a human subject via a selected administration route, the supplement formulation comprising therapeutically effective amounts of a hormone supplement, a plurality of collagen peptides, and a plurality of elastin peptides. The hormone supplement may comprise either HGH or IGF-1, or precursors or analogues thereof, and the collagen peptides may be sourced from a plurality of animal-based sources, which may include at least one of chicken bone, bovine hide, or fish tissues.

The invention provides an O-type foot-and-mouth disease nanoparticle antigen NP-OVPn as well as a preparation method and application thereof. The NP-OVPn provided by the invention is obtained by the following steps: after intracellular self-assembled O-type foot-and-mouth disease nanoparticle antigen NP-OVPn-T is subjected to TEV enzyme cleavage, removing T fusion-promoting tag protein through nickel affinity chromatography purification, and enabling antigen epitopes to be fully exposed on the surface of nanoparticle carrier protein NP. According to the FMDV O-type NP-OVPn nanoparticle antigen, three structural proteins of FMDV O-type VP1, VP2 and VP3 are displayed at the same time in the form of nanoparticles, the FMDV O-type NP-OVPn nanoparticle antigen can well and uniformly react with antibodies generated after immunization or infection of three animals including pigs, cattle and sheep, and the problem that the source of the FMDV O-type antigen is limited in FMDV clinical diagnosis is solved. The invention also provides a vaccine developed on the basis of the NP-OVPn nanoparticle antigen, and an FMDV O-type LB-ELISA antibody detection kit. The kit can be simultaneously used for quantitative detection of FMDV O-type specific antibodies from three different animals, namely pigs, cows and sheep, and has the advantages of high sensitivity, good specificity and high accuracy.

A masking composition for masking anomalous characteristics of proteins of non-animal origin is provided. The masking composition comprises a) a mixture of a labiatae extract and vitamin C, wherein the mixing ratio of the extract to the vitamin C is 1: 1 to 1: 20; b) one or more peptides; c) an extract obtained or obtainable from a plant of the genus Coffee; d) a modifier imparting umami; and e) one or more masking agents selected from the group consisting of fatty acids, terpenes, carbonyl compounds, sulfur compounds, sweet brown substances, esters, sweeteners, lactones, juice derivatives, and combinations thereof.

The invention provides a fermentation flavoring agent for dogs and a preparation method thereof, and the preparation method comprises the following steps: (1) raw material pretreatment: animal source treatment: taking 40-50 parts of dog preferred animal source raw materials, beating, coating animal fat ointment, and then mincing; carrying out plant source treatment: taking 15-25 parts of plant source raw materials, and carrying out superfine grinding to 60-120 meshes; (2) directional enzymolysis: carrying out enzymolysis on the animal-derived raw material and compound enzyme for 1.5-3 hours under the conditions that the temperature is 40-55 DEG C and the pH value is 5.8-6.8, wherein the compound enzyme consists of flavourzyme, papain and neutral protease according to the ratio of 1: (1.5-2.5): (0.3-0.7); and (3) three-stage stepped fermentation. The prepared fermented flavor agent for dogs has high palatability, strong digestion aiding ability, excellent intestinal protection effect and good storage stability, and is suitable for the requirements of dogs and industrial application.

The invention discloses a preparation method of Brassica rapa L.-derived extracellular vesicle-like particles and an application of the Brassica rapa L.-derived extracellular vesicle-like particles in pulmonary fibrosis resistance. The preparation method comprises the following steps: raw material pretreatment and homogenate preparation: cleaning and grinding the common turnip, adding the common turnip into PBS (Phosphate Buffer Solution) which is 1.5 times of the mass of the medicinal material, soaking for 180 minutes, putting into a homogenizer to prepare homogenate, filtering, standing, and taking supernatant; performing super-gradient centrifugal purification: performing gradient centrifugation, taking supernatant and discarding precipitate; carrying out ultra-high-speed centrifugal collection, namely carrying out ultra-high-speed centrifugal collection on the supernatant at 120000g for 120 minutes and taking precipitate; and re-suspending and storing: re-rotating and precipitating by using PBS to obtain BREVLPs, and storing at ultralow temperature for later use. The extracellular vesicle-like particles (BREVLPs) are prepared from the common turnip for the first time, the preparation method is simple in process, convenient to operate, wide in source of the raw material common turnip, low in cost and suitable for large-scale production, and the problems that existing animal-derived extracellular vesicle-like particles are high in culture cost and complex in process are solved.