84 results about "Animal Sources" patented technology

Relating to the field of biotechnology, disclosed are a recombinant human elastin peptide with anti-aging effects and a use thereof. The recombinant human elastin peptide comprises an amino acid sequence shown in SEQ ID NO:1 or a fragment of the amino acid sequence shown in SEQ ID NO:1, the fragment comprises 30% or more of SEQ ID NO:1, or a mutant of the amino acid sequence shown in SEQ ID NO:1 or the fragment of the amino acid sequence shown in SEQ ID NO:1. A disclosed method for producing elastin peptides by using a yeast expression system enables the targeted synthesis of highly effective protein fragments with 100% homology to human proteins, while achieving low costs and facilitating large-scale production, avoiding animal-derived sources and viral risks. This makes the recombinant human elastin peptide safe for use as an anti-aging and anti-photoaging active ingredient in cosmetics, while also supporting a use thereof in the field of biomedical materials.

The invention discloses synthesized XVII type humanized collagen as well as a preparation method and application thereof, and particularly relates to the technical field of genetic engineering. The amino acid sequence of the recombinant XVII type collagen is as shown in SEQ ID NO. 1. According to the invention, key functional domains (five high-hydrophilicity and high-stability peptide fragments such as 641-685 and the like) in a human XVII type collagen alpha1 chain are retained, and non-functional regions are removed. The 100% human source sequence coverage rate is adopted, the immunogenicity risk of animal source collagen is completely avoided, and the biocompatibility is excellent. The functional peptide fragment is repeated twice to form a stable structure of 210 amino acids, so that the functional activity of the protein is enhanced. The theoretical isoelectric point (pI = 6.07) is close to the pH value (7.4) of human tissue fluid, the dissolvability is excellent, and the applicability is high.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.

The present invention relates to the field of biotechnology and discloses a recombinant human elastin peptide with anti-aging efficacy and its application in a composition thereof. The recombinant human elastin peptide comprises the elastin peptide amino acid sequence SEQ ID: 1 or a fragment thereof, wherein the fragment comprises 30% of SEQ ID: 1, or a fragment comprises more than 30% of SEQ ID: 1, or a mutant of the above sequence. The recombinant human elastin peptide can be directed to synthesize a protein of a highly effective fragment, with a 100% human homologous sequence, while being low-cost, easy to mass-produce, avoiding animal sources, and avoiding viral risks. This allows the recombinant human elastin peptide to be safely used in cosmetics as an anti-aging and anti-photoaging ingredient, while also providing support for its application in the field of biomedical materials.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

A foodstuff comprises animal-derived meat and a filamentous fungus, for example Fusarium venenatum. It may comprise: 3.0 to 25.0 wt% animal-derived meat on a dry mass basis; 2.0 to 25.0 wt% fungus on a dry mass basis; 50.0 to 90.0 wt% water; 0.0 to 6.0 wt% flavourant(s); and 0.0 to 15.0 wt% binder(s).

The application discloses a freeze-drying protective agent for Streptococcus pneumoniae without animal source, and belongs to the field of microorganism preservation. The freeze-drying protective agent is composed of the following components: 1-5% (weight / volume) soybean peptone, 1-5% (weight / volume) trehalose, 4-8% (weight / volume) mannitol and 0.3-1.5% (volume / volume) glycerol, and the rest is water. The freeze-drying protective agent can better maintain the activity of Streptococcus pneumoniae, and the effect is equivalent to that of a skimmed milk protective agent or a commercially available freeze-drying protective agent without animal source, but the freeze-drying protective agent does not contain animal source components and has low cost.

The present invention relates to a method for obtaining a fertiliser by pyrolysis of raw materials of animal origin, in particular selected from hair, feathers, wool, or meat meal. The product obtained by said pyrolysis can be used directly as a fertiliser or as a raw material for obtaining fertilisers.

A pharmaceutical composition comprising: an active pharmaceutical ingredient (API); 1-45 wt% non-gelatin matrix former comprising hydroxypropyl methylcellulose (hypromellose, HPMC), methyl cellulose,

The present invention provides a textured plant protein defined as a fibrous plant protein material having a protein content of at least 40 wt.% relative to dry matter wherein the textured plant protein is prepared by extruding a feed composition at a moisture content of 18.1-25.9 wt.%, the feed composition comprises: a) a source of plant protein from seeds, cereals, algae, fruits, leaves or legumes, comprising at least 65 wt.% of plant protein relative to dry matter; and b) a source of tuber protein comprising at least 75 wt.% of tuber protein relative to dry matter, and c) a filler, In addition, the invention provides food products comprising the TVP of the invention, as well as methods for obtaining these products, and the use of TVP in food products to replace meat of animal origin.

The present invention relates to an ACCS gene, a protein and the use thereof. An involved ACCS protein mutant is formed by means of mutating a proline residue at position 134 and a tryptophan residue at position 136 of an animal-derived wild-type ACCS protein into a glutamine residue and a tyrosine residue, respectively. The involved ACCS protein mutant can be used in the intervention and treatment of a variety of diseases comprising brain diseases, and provides a new regimen and approach for the intervention and treatment of brain diseases.

The invention relates to the technical field of hydrogel, in particular to diseased animal source amino acid nutritional hydrogel for smart agriculture and a preparation method thereof.The diseased animal source amino acid serves as a nutritional ingredient, and the diseased animal source amino acid is stably integrated into a hydrogel network through photo-initiation through modification treatment; the preparation method comprises the following steps: by taking multi-aldehyde hemp cellulose as a carrier, grafting with compound amino acid through Schiff base reaction to obtain grafted compound amino acid; then taking the grafted compound amino acid as a core material and starch octenylsuccinate as a wall material to prepare a compound amino acid microcapsule; one of polyvinyl alcohol, polyethylene glycol, polyacrylamide and polyvinylpyrrolidone is used as an additive to be compounded with a conventional fertilizer; arabic gum, carboxymethyl chitosan and sodium alginate are compounded to serve as a degradable hydrogel matrix, acrylic acid is grafted to a polymer network, compound amino acid microcapsules with double bonds on the surfaces serve as nutritional ingredients, and the hydrogel is prepared through light-induced polymerization.

The invention belongs to the technical field of cell biology, and particularly relates to a method for amplifying clinical-grade hair follicle stem cells on a large scale and application of the clinical-grade hair follicle stem cells in preparation of products for treating alopecia. According to the method provided by the invention, stable amplification of the hair follicle stem cells can be realized by accurately controlling passage times, and reagents used in the culture process do not contain animal sources, so that the possibility of animal source pathogen pollution does not exist. The clinical-grade hair follicle stem cells amplified in a large scale by the method provided by the invention can provide high-quality cell resources for alopecia patients, can be used for hair follicle regeneration treatment, and can be transplanted to an alopecia area to stimulate hair follicle regeneration so as to realize regeneration and growth of hair.

The invention provides a fermentation flavoring agent for dogs and a preparation method thereof, and the preparation method comprises the following steps: (1) raw material pretreatment: animal source treatment: taking 40-50 parts of dog preferred animal source raw materials, beating, coating animal fat ointment, and then mincing; carrying out plant source treatment: taking 15-25 parts of plant source raw materials, and carrying out superfine grinding to 60-120 meshes; (2) directional enzymolysis: carrying out enzymolysis on the animal-derived raw material and compound enzyme for 1.5-3 hours under the conditions that the temperature is 40-55 DEG C and the pH value is 5.8-6.8, wherein the compound enzyme consists of flavourzyme, papain and neutral protease according to the ratio of 1: (1.5-2.5): (0.3-0.7); and (3) three-stage stepped fermentation. The prepared fermented flavor agent for dogs has high palatability, strong digestion aiding ability, excellent intestinal protection effect and good storage stability, and is suitable for the requirements of dogs and industrial application.

The invention discloses a preparation method of Brassica rapa L.-derived extracellular vesicle-like particles and an application of the Brassica rapa L.-derived extracellular vesicle-like particles in pulmonary fibrosis resistance. The preparation method comprises the following steps: raw material pretreatment and homogenate preparation: cleaning and grinding the common turnip, adding the common turnip into PBS (Phosphate Buffer Solution) which is 1.5 times of the mass of the medicinal material, soaking for 180 minutes, putting into a homogenizer to prepare homogenate, filtering, standing, and taking supernatant; performing super-gradient centrifugal purification: performing gradient centrifugation, taking supernatant and discarding precipitate; carrying out ultra-high-speed centrifugal collection, namely carrying out ultra-high-speed centrifugal collection on the supernatant at 120000g for 120 minutes and taking precipitate; and re-suspending and storing: re-rotating and precipitating by using PBS to obtain BREVLPs, and storing at ultralow temperature for later use. The extracellular vesicle-like particles (BREVLPs) are prepared from the common turnip for the first time, the preparation method is simple in process, convenient to operate, wide in source of the raw material common turnip, low in cost and suitable for large-scale production, and the problems that existing animal-derived extracellular vesicle-like particles are high in culture cost and complex in process are solved.

The present invention relates to a cell culture medium comprising a hydrolysate composition. In particular, the hydrolysate composition is a plant protein hydrolysate, prepared from soy or wheat enzymatically hydrolysed with alcalase, flavourzyme, neutrase, or protamex. The cell culture medium is suited for culturing animal-derived cells in cultivated meat production, where it offers a cost-effective and sustainable alternative to conventional formulations, facilitating broader commercial adoption. Also disclosed is a method of preparing the cell culture medium.

The present invention relates to a vegan and reusable leather type material of vegetable origin, at least 50% and preferably at least 80% biobased. This material is based on a composition which comprises persimmon (Diospyros kaki) mass and a polymer derived from a polyol of vegetable origin. The invention also discloses the process for obtaining the material. The leather-type material may be used in a variety of goods products in the textile such as clothes, footwear products or accessories. The material has proven to keep the same mechanical properties as the animal origin or synthetic leather, being a sustainable alternative which, in addition, contributes to circular economy. The sustainable material, leather-type of vegetable origin, provides an alternative to known leathers, with less environmental impact.

The present invention relates to macromolecular blockers of non-specific interactions of an analyte and other components of the analytical system with the solid phase, which considerably decreases the non-specific interactions during the assay. The macromolecular blocker comprises synthetic copolymer or homopolymer, selected from the group comprising polyacrylamide, polymethacrylamide, polyacrylate, polymethacrylate, poly(N-(2-hydroxypropyl)methacrylamide), poly(2-oxazoline); to which at least one hydrophobically-active anchor is attached via a covalent bond, said hydrophobically-active anchor enabling suppression of non-specific interactions in immuno assays. The invention further relates to a method of preparing and to the use of said macromolecular blockers for capturing of specific antibodies or antigens or other molecules on the surface of the solid phase. The present invention enables to replace animal-derived proteins with a synthetic macromolecular blocker that is not only more effective in blocking activity itself, but it is also defined in its structure, has high batch-to-batch reproducibility, and does not need to be tested for the presence of viruses and other pathogens.

The invention belongs to the technical field of biological product extraction, and particularly relates to a bovine-derived Muslim chondroitin sulfate and collagen peptide co-production method based on a composite enzymolysis-membrane separation coupling technology, which comprises the steps of pretreatment of bovine cartilage, enzymolysis, membrane analysis and filtration, extraction of chondroitin and collagen peptide and the like. According to the method, animal source enzyme is replaced by microbial enzyme, religious taboo is avoided, resources are efficiently utilized, two high-value products are synchronously extracted, and the raw material cost is reduced; a membrane separation technology replaces an organic solvent, and pollution is reduced.

The invention provides fish-algae paste as well as a preparation method and application thereof, and relates to the field of feed additives. The preparation method of the fish-algae paste comprises the following steps of anchovy pretreatment, algae pretreatment and enzymatic reaction. According to the fish-algae paste as well as the preparation method and the application thereof, animal source components and algae source components are effectively combined, so that adverse effects on effective components in the preparation process can be effectively avoided, and the food calling performance and the health-care gain performance of the fish-algae paste are synchronously improved.

The present invention belongs to the field of biotechnology, in particular the field of biochemical composition and process of preparation thereof. The present invention more particularly relates to composition of ingredients as a serum alternative from an animal origin free source for fetal bovine serum and development of serum alternative from the same. More particularly, the present invention further relates to the specific composition as a serum alternative and application thereof in animal cell culture, biopharmaceutical ingredients in antibody and vaccine preparation, formulation of final drug components and other translation research work like stem cell research.

The invention discloses a serum-free culture medium for culturing Vero cells. The serum-free culture medium comprises a DMEM (Dulbecco Modified Eagle Medium), an ultrafiltration type animal-source-free soybean protein hydrolysate and a TE02 additive, the content of the DMEM culture medium is 13380 mg / L, the content of the ultrafiltration type animal-source-free soybean protein hydrolysate is 1000 to 5000 mg / L, and the content of the TE02 additive is 0.2 to 0.5 mL / L; the serum-free culture medium disclosed by the invention is superior to commercial like products in cell growth morphology, amplification multiple and passage stability; the serum-free culture can be completely supported in the cell culture process, so that the influence caused by the quality difference between different batches of serum is avoided, and the blood-derived pollution is avoided; the batch-to-batch difference in the large-scale production process is easy to control, the culture medium is stable in property, and the transportation and preservation conditions are relatively loose; the feed is clear in components, free of animal-derived components, low in protein content and high in safety.

A rotor (8) for a machine (1) for extracting puree or juice from a food product of vegetable or animal origin, being provided with a hub (11) having an external conical, or cylindrical, surface (13) from which a plurality of blades (6, Bn,m) protrudes, in which the hub (11) and the blades (6) form a monolithic body obtained with a process selected from: welding of the blades to the hub, machining of the blades and the hub from a monolithic semi-finished product. The blades (6) are arranged according to a matrix Bn,m where n is the number of blades (6) in the longitudinal direction (9) and m is the number of blades (6) along a circumferential direction, where n>≤2 and m≤2; the blades Bn,m have at least a concave portion with a concavity oriented in the direction of rotation (10) of the rotor (8). The hub (11) has an axial length (L) and each of the blades Bn,m has an axial length A less than L / n with n≤2.

To provide an immediately usable composition for subcutaneous or intradermal injection, and a method for performing restoration or cosmetic dermatology treatment.SOLUTION: Provided is an immediately usable injectable composition containing polymeric microspheres or fine particles originated from non-animal, hydrogel containing water and cellulose derivative gelatinizer, and polysorbate 80. Further provided is a method for using an immediately usable injectable composition for restoration or formation surgery, cosmetic dermatology, contour formation of a face, contour formation of a body, and gum augmentation.SELECTED DRAWING: None

The present disclosure is directed to a coated paper. The coated paper includes a cellulose layer and at least two coatings on one side of the paper. The first coating applied to the paper is a barrier coating that can be made from a plant or animal derived component, which is preferably water-soluble or water-dispersible, and more preferably water-soluble. The second coating, on the other hand, can be a heat-sealable coating. The coated paper is particularly well suited to constructing packages.

The application discloses a method for accurately quantitatively detecting sheep, pig, chicken and duck derived components in meat food based on real-time fluorescent PCR and application, and the method comprises the following steps: (1) designing upstream primers, downstream primers and probes of sheep, pig, chicken and duck marker genes; (2) extracting DNA of sheep, pig, chicken and duck standard substances and test samples respectively; (3) gradient dilution is conducted on the DNA of the sheep, pig, chicken and duck standard substances extracted in the step (2), a standard curve of the sheep, pig, chicken and duck is drawn, and a standard curve equation y=ax+b is fitted; (4) taking the DNA of the test sample extracted in the step (2) as a template, real-time fluorescent PCR amplification is conducted; the copy number of each animal source marker gene is calculated according to the standard curve equation in the step (3); and (5) the percentage content of the animal derived components is calculated according to the formula:

This invention relates to the field of stem cell culture technology, and more particularly to compositions and their applications in stem cell culture. The invention utilizes a composition formed by combining acetate, a pyruvate dehydrogenase kinase (PDK) specific inhibitor, and an HMG-CoA reductase inhibitor to enhance the ability of mesenchymal stem cells to maintain stemness and prevent differentiation during passage. Based on this composition, further incorporating various nutrients, the resulting composition provides ample nutrition to mesenchymal stem cells, maintaining their good proliferative capacity and stemness during passage, even in the absence of serum and animal-derived components, with batch-to-batch stability.