111 results about "Animal Sources" patented technology

The invention relates to method for obtaining collagen from animal sources such as fresh or saltwater fish, chicken, eggs or from parts of these animal sources that can be considered waste, and to a collagen hydrolysate obtained as a result of this method.

Relating to the field of biotechnology, disclosed are a recombinant human elastin peptide with anti-aging effects and a use thereof. The recombinant human elastin peptide comprises an amino acid sequence shown in SEQ ID NO:1 or a fragment of the amino acid sequence shown in SEQ ID NO:1, the fragment comprises 30% or more of SEQ ID NO:1, or a mutant of the amino acid sequence shown in SEQ ID NO:1 or the fragment of the amino acid sequence shown in SEQ ID NO:1. A disclosed method for producing elastin peptides by using a yeast expression system enables the targeted synthesis of highly effective protein fragments with 100% homology to human proteins, while achieving low costs and facilitating large-scale production, avoiding animal-derived sources and viral risks. This makes the recombinant human elastin peptide safe for use as an anti-aging and anti-photoaging active ingredient in cosmetics, while also supporting a use thereof in the field of biomedical materials.

The invention discloses synthesized XVII type humanized collagen as well as a preparation method and application thereof, and particularly relates to the technical field of genetic engineering. The amino acid sequence of the recombinant XVII type collagen is as shown in SEQ ID NO. 1. According to the invention, key functional domains (five high-hydrophilicity and high-stability peptide fragments such as 641-685 and the like) in a human XVII type collagen alpha1 chain are retained, and non-functional regions are removed. The 100% human source sequence coverage rate is adopted, the immunogenicity risk of animal source collagen is completely avoided, and the biocompatibility is excellent. The functional peptide fragment is repeated twice to form a stable structure of 210 amino acids, so that the functional activity of the protein is enhanced. The theoretical isoelectric point (pI = 6.07) is close to the pH value (7.4) of human tissue fluid, the dissolvability is excellent, and the applicability is high.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer and a method for detecting salmonella and a drug-resistant gene thereof and application of the primer and the method. The gene of the salmonella is invA, and the drug resistance genes are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the gene of the salmonella is invA, and the drug resistance genes of the salmonella are blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA; the primers comprise inner primers and outer primers of invA, blaCTX-M, blaNDM-1, blaTEM-1 and blaOXA, a primer group is designed according to conserved regions of a salmonella invA gene and part of beta-lactam drug-resistant genes blaNDM-1, blaTEM-1, blaOXA and blaCTX-M, a detection method of SYBR-LAMP and HNB-LAMP technologies is established, and screening of part of beta-lactam drug-resistant genes carried by animal-derived pathogenic strains is realized.

A method of decellularization of animal tissue, comprising the steps of: mechanical homogenization of animal-derived tissue, incubation of the homogenized tissue from step a) in a solution of trypsin and EDTA, subjecting the filtered tissue to incubation in a PBS buffer solution with a pH in the range of 7.2-7.6 containing a non-ionic detergent at a concentration of 4 - 2% and NH4OH at a concentration of 0.2 - 0.08%, rinsing the tissue with a PBS buffer solution containing an antimicrobial agent, incubation in a deoxyribonuclease solution at a concentration of 0.001 to 0.003% (w / v), rinsing the decellularized tissues obtained with a PBS buffer solution containing an antimicrobial agent, wherein the non-ionic detergent is a compound with a molecular weight of 596 g / mol and additional steps of incubating the tissue in the solution PBS buffer with a pH of 7.2- 7.6 containing a non-ionic detergent at a concentration of 1.5-0.5% and NH4OH ata concentration of 0.2-0.08%, where the incubation is carried out at a temperature below room temperature for 36-48h, and incubation of the tissue in a PBS buffer solution with a pH of 7.2-7.6 containing a non- ionic detergent at a concentration of 0.75-0.25% and NH4OH at a concentration of 0.2-0.08%, where the incubation is carried out at a temperature below room temperature for 36-48h. Bioink containing decellularized tissue of animal origin.

The present invention relates to the field of biotechnology and discloses a recombinant human elastin peptide with anti-aging efficacy and its application in a composition thereof. The recombinant human elastin peptide comprises the elastin peptide amino acid sequence SEQ ID: 1 or a fragment thereof, wherein the fragment comprises 30% of SEQ ID: 1, or a fragment comprises more than 30% of SEQ ID: 1, or a mutant of the above sequence. The recombinant human elastin peptide can be directed to synthesize a protein of a highly effective fragment, with a 100% human homologous sequence, while being low-cost, easy to mass-produce, avoiding animal sources, and avoiding viral risks. This allows the recombinant human elastin peptide to be safely used in cosmetics as an anti-aging and anti-photoaging ingredient, while also providing support for its application in the field of biomedical materials.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

A foodstuff comprises animal-derived meat and a filamentous fungus, for example Fusarium venenatum. It may comprise: 3.0 to 25.0 wt% animal-derived meat on a dry mass basis; 2.0 to 25.0 wt% fungus on a dry mass basis; 50.0 to 90.0 wt% water; 0.0 to 6.0 wt% flavourant(s); and 0.0 to 15.0 wt% binder(s).

The invention relates to the technical field of medicines, in particular to novel application of galactooligosaccharide. The novel use of galactooligosaccharide inhibits the formation of gallstone. Main reagents and materials are galactooligosaccharide; a mouse calculus model feed; the experimental animals are derived from animal varieties and strains: mice, C57BL / 6J; sex: male; week age: 4 week age; the body weight is 16-20 g; the experimental environment is SPF environment feeding, and the feeding condition is that the room temperature is 20-26 DEG C; the relative humidity is 40-70%; automatic illumination is achieved, and light and shade are alternated for 12 h; the drinking water is RO membrane reverse osmosis sterile water; the feeding density is defaulted to be 4 per cage, and the padding is replaced once a week; the experimental process comprises the following steps: starting an experiment after experimental feeding of all mice for one week, and randomly dividing 36 5-week male C57BL / 6J mice into three groups, namely 12 mice in each group. The invention provides a new application of galactooligosaccharide, which can inhibit the formation of gallstone and provides a new thought for preventing the occurrence of the gallstone.

The application discloses a freeze-drying protective agent for Streptococcus pneumoniae without animal source, and belongs to the field of microorganism preservation. The freeze-drying protective agent is composed of the following components: 1-5% (weight / volume) soybean peptone, 1-5% (weight / volume) trehalose, 4-8% (weight / volume) mannitol and 0.3-1.5% (volume / volume) glycerol, and the rest is water. The freeze-drying protective agent can better maintain the activity of Streptococcus pneumoniae, and the effect is equivalent to that of a skimmed milk protective agent or a commercially available freeze-drying protective agent without animal source, but the freeze-drying protective agent does not contain animal source components and has low cost.

Compositions comprising oils of plant or animal origin, such as fatty acid alkyl esters, are stabilized against oxidation using oxidized aminophenol antioxidants. The aminophenol includes one or two carbon-containing groups including hydroxyl groups and / or ether groups separated from an N atom by one or more carbon atoms. The oxidized aminophenol antioxidants effectively control oxidation in moderate and even low amounts, can be used in a variety of different oil and fatty acid methyl ester formulations, and are effective at elevated temperatures, and exhibit significantly superior performance to conventional antioxidants.

The present invention relates to a method for obtaining a fertiliser by pyrolysis of raw materials of animal origin, in particular selected from hair, feathers, wool, or meat meal. The product obtained by said pyrolysis can be used directly as a fertiliser or as a raw material for obtaining fertilisers.

A pharmaceutical composition comprising: an active pharmaceutical ingredient (API); 1-45 wt% non-gelatin matrix former comprising hydroxypropyl methylcellulose (hypromellose, HPMC), methyl cellulose,

The present invention provides a textured plant protein defined as a fibrous plant protein material having a protein content of at least 40 wt.% relative to dry matter wherein the textured plant protein is prepared by extruding a feed composition at a moisture content of 18.1-25.9 wt.%, the feed composition comprises: a) a source of plant protein from seeds, cereals, algae, fruits, leaves or legumes, comprising at least 65 wt.% of plant protein relative to dry matter; and b) a source of tuber protein comprising at least 75 wt.% of tuber protein relative to dry matter, and c) a filler, In addition, the invention provides food products comprising the TVP of the invention, as well as methods for obtaining these products, and the use of TVP in food products to replace meat of animal origin.

The present invention relates to an ACCS gene, a protein and the use thereof. An involved ACCS protein mutant is formed by means of mutating a proline residue at position 134 and a tryptophan residue at position 136 of an animal-derived wild-type ACCS protein into a glutamine residue and a tyrosine residue, respectively. The involved ACCS protein mutant can be used in the intervention and treatment of a variety of diseases comprising brain diseases, and provides a new regimen and approach for the intervention and treatment of brain diseases.

The invention relates to the technical field of hydrogel, in particular to diseased animal source amino acid nutritional hydrogel for smart agriculture and a preparation method thereof.The diseased animal source amino acid serves as a nutritional ingredient, and the diseased animal source amino acid is stably integrated into a hydrogel network through photo-initiation through modification treatment; the preparation method comprises the following steps: by taking multi-aldehyde hemp cellulose as a carrier, grafting with compound amino acid through Schiff base reaction to obtain grafted compound amino acid; then taking the grafted compound amino acid as a core material and starch octenylsuccinate as a wall material to prepare a compound amino acid microcapsule; one of polyvinyl alcohol, polyethylene glycol, polyacrylamide and polyvinylpyrrolidone is used as an additive to be compounded with a conventional fertilizer; arabic gum, carboxymethyl chitosan and sodium alginate are compounded to serve as a degradable hydrogel matrix, acrylic acid is grafted to a polymer network, compound amino acid microcapsules with double bonds on the surfaces serve as nutritional ingredients, and the hydrogel is prepared through light-induced polymerization.

The invention belongs to the technical field of cell biology, and particularly relates to a method for amplifying clinical-grade hair follicle stem cells on a large scale and application of the clinical-grade hair follicle stem cells in preparation of products for treating alopecia. According to the method provided by the invention, stable amplification of the hair follicle stem cells can be realized by accurately controlling passage times, and reagents used in the culture process do not contain animal sources, so that the possibility of animal source pathogen pollution does not exist. The clinical-grade hair follicle stem cells amplified in a large scale by the method provided by the invention can provide high-quality cell resources for alopecia patients, can be used for hair follicle regeneration treatment, and can be transplanted to an alopecia area to stimulate hair follicle regeneration so as to realize regeneration and growth of hair.

A method and compositions of treatment for improving skin health and sleep quality using supplementation, comprising the step of applying a supplement formulation to a human subject via a selected administration route, the supplement formulation comprising therapeutically effective amounts of a hormone supplement, a plurality of collagen peptides, and a plurality of elastin peptides. The hormone supplement may comprise either HGH or IGF-1, or precursors or analogues thereof, and the collagen peptides may be sourced from a plurality of animal-based sources, which may include at least one of chicken bone, bovine hide, or fish tissues.

The invention provides an O-type foot-and-mouth disease nanoparticle antigen NP-OVPn as well as a preparation method and application thereof. The NP-OVPn provided by the invention is obtained by the following steps: after intracellular self-assembled O-type foot-and-mouth disease nanoparticle antigen NP-OVPn-T is subjected to TEV enzyme cleavage, removing T fusion-promoting tag protein through nickel affinity chromatography purification, and enabling antigen epitopes to be fully exposed on the surface of nanoparticle carrier protein NP. According to the FMDV O-type NP-OVPn nanoparticle antigen, three structural proteins of FMDV O-type VP1, VP2 and VP3 are displayed at the same time in the form of nanoparticles, the FMDV O-type NP-OVPn nanoparticle antigen can well and uniformly react with antibodies generated after immunization or infection of three animals including pigs, cattle and sheep, and the problem that the source of the FMDV O-type antigen is limited in FMDV clinical diagnosis is solved. The invention also provides a vaccine developed on the basis of the NP-OVPn nanoparticle antigen, and an FMDV O-type LB-ELISA antibody detection kit. The kit can be simultaneously used for quantitative detection of FMDV O-type specific antibodies from three different animals, namely pigs, cows and sheep, and has the advantages of high sensitivity, good specificity and high accuracy.

A masking composition for masking anomalous characteristics of proteins of non-animal origin is provided. The masking composition comprises a) a mixture of a labiatae extract and vitamin C, wherein the mixing ratio of the extract to the vitamin C is 1: 1 to 1: 20; b) one or more peptides; c) an extract obtained or obtainable from a plant of the genus Coffee; d) a modifier imparting umami; and e) one or more masking agents selected from the group consisting of fatty acids, terpenes, carbonyl compounds, sulfur compounds, sweet brown substances, esters, sweeteners, lactones, juice derivatives, and combinations thereof.

The invention provides a fermentation flavoring agent for dogs and a preparation method thereof, and the preparation method comprises the following steps: (1) raw material pretreatment: animal source treatment: taking 40-50 parts of dog preferred animal source raw materials, beating, coating animal fat ointment, and then mincing; carrying out plant source treatment: taking 15-25 parts of plant source raw materials, and carrying out superfine grinding to 60-120 meshes; (2) directional enzymolysis: carrying out enzymolysis on the animal-derived raw material and compound enzyme for 1.5-3 hours under the conditions that the temperature is 40-55 DEG C and the pH value is 5.8-6.8, wherein the compound enzyme consists of flavourzyme, papain and neutral protease according to the ratio of 1: (1.5-2.5): (0.3-0.7); and (3) three-stage stepped fermentation. The prepared fermented flavor agent for dogs has high palatability, strong digestion aiding ability, excellent intestinal protection effect and good storage stability, and is suitable for the requirements of dogs and industrial application.

The invention discloses a preparation method of Brassica rapa L.-derived extracellular vesicle-like particles and an application of the Brassica rapa L.-derived extracellular vesicle-like particles in pulmonary fibrosis resistance. The preparation method comprises the following steps: raw material pretreatment and homogenate preparation: cleaning and grinding the common turnip, adding the common turnip into PBS (Phosphate Buffer Solution) which is 1.5 times of the mass of the medicinal material, soaking for 180 minutes, putting into a homogenizer to prepare homogenate, filtering, standing, and taking supernatant; performing super-gradient centrifugal purification: performing gradient centrifugation, taking supernatant and discarding precipitate; carrying out ultra-high-speed centrifugal collection, namely carrying out ultra-high-speed centrifugal collection on the supernatant at 120000g for 120 minutes and taking precipitate; and re-suspending and storing: re-rotating and precipitating by using PBS to obtain BREVLPs, and storing at ultralow temperature for later use. The extracellular vesicle-like particles (BREVLPs) are prepared from the common turnip for the first time, the preparation method is simple in process, convenient to operate, wide in source of the raw material common turnip, low in cost and suitable for large-scale production, and the problems that existing animal-derived extracellular vesicle-like particles are high in culture cost and complex in process are solved.

The present invention relates to a cell culture medium comprising a hydrolysate composition. In particular, the hydrolysate composition is a plant protein hydrolysate, prepared from soy or wheat enzymatically hydrolysed with alcalase, flavourzyme, neutrase, or protamex. The cell culture medium is suited for culturing animal-derived cells in cultivated meat production, where it offers a cost-effective and sustainable alternative to conventional formulations, facilitating broader commercial adoption. Also disclosed is a method of preparing the cell culture medium.

The present invention relates to a vegan and reusable leather type material of vegetable origin, at least 50% and preferably at least 80% biobased. This material is based on a composition which comprises persimmon (Diospyros kaki) mass and a polymer derived from a polyol of vegetable origin. The invention also discloses the process for obtaining the material. The leather-type material may be used in a variety of goods products in the textile such as clothes, footwear products or accessories. The material has proven to keep the same mechanical properties as the animal origin or synthetic leather, being a sustainable alternative which, in addition, contributes to circular economy. The sustainable material, leather-type of vegetable origin, provides an alternative to known leathers, with less environmental impact.

The present invention relates to macromolecular blockers of non-specific interactions of an analyte and other components of the analytical system with the solid phase, which considerably decreases the non-specific interactions during the assay. The macromolecular blocker comprises synthetic copolymer or homopolymer, selected from the group comprising polyacrylamide, polymethacrylamide, polyacrylate, polymethacrylate, poly(N-(2-hydroxypropyl)methacrylamide), poly(2-oxazoline); to which at least one hydrophobically-active anchor is attached via a covalent bond, said hydrophobically-active anchor enabling suppression of non-specific interactions in immuno assays. The invention further relates to a method of preparing and to the use of said macromolecular blockers for capturing of specific antibodies or antigens or other molecules on the surface of the solid phase. The present invention enables to replace animal-derived proteins with a synthetic macromolecular blocker that is not only more effective in blocking activity itself, but it is also defined in its structure, has high batch-to-batch reproducibility, and does not need to be tested for the presence of viruses and other pathogens.

The invention belongs to the technical field of biological product extraction, and particularly relates to a bovine-derived Muslim chondroitin sulfate and collagen peptide co-production method based on a composite enzymolysis-membrane separation coupling technology, which comprises the steps of pretreatment of bovine cartilage, enzymolysis, membrane analysis and filtration, extraction of chondroitin and collagen peptide and the like. According to the method, animal source enzyme is replaced by microbial enzyme, religious taboo is avoided, resources are efficiently utilized, two high-value products are synchronously extracted, and the raw material cost is reduced; a membrane separation technology replaces an organic solvent, and pollution is reduced.

The invention provides fish-algae paste as well as a preparation method and application thereof, and relates to the field of feed additives. The preparation method of the fish-algae paste comprises the following steps of anchovy pretreatment, algae pretreatment and enzymatic reaction. According to the fish-algae paste as well as the preparation method and the application thereof, animal source components and algae source components are effectively combined, so that adverse effects on effective components in the preparation process can be effectively avoided, and the food calling performance and the health-care gain performance of the fish-algae paste are synchronously improved.