22 results about "Methyl donor" patented technology

The invention relates to a methyltransferase gene for catalyzing methylation of resveratrol and application of the methyltransferase gene, and belongs to the technical field of biology. The invention provides a gene of methyltransferase capable of catalyzing resveratrol to generate pinostilbene and pterostilbene. The gene of the methyltransferase is a gene YC26, a gene SS15 or a gene HS97-5. The methyltransferase gene provided by the invention has the advantages of high substrate conversion rate, rich products, high activity and the like, the conversion rate of resveratrol can be increased and synthesis of pterostilbene can be promoted when the methyltransferase gene is combined with other methyltransferase genes to express in different strains, additional addition of expensive methyl donors is avoided, and the production cost is reduced. Only a small amount of substrate resveratrol needs to be added or no substrate resveratrol is added.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

Non-therapeutic method for imparting a controlled tanning to the skin, comprising the step of applying to the skin a composition comprising a first compound A, which is a methyl group donor, and a second compound B, which is an acetyl-CoA donor.

The invention relates to a method for preparing irisflorentin by utilizing belamcandin, and belongs to the technical field of biology. The method comprises the following steps: carrying out C-5 site and C-3'site methylation reaction by taking white belamcandin as a substrate, taking belamcanda methyl transferase gene BcOMT03 gene encoding protein and belamcanda methyl transferase BcOMT33 gene encoding protein as catalysts and taking S-adenosine-methionine as a methyl donor to generate a corresponding glycosylation product. According to the invention, the functions of the two methyltransferase genes BcOMT03 and BcOMT33 in the blackberry lily are identified and verified for the first time, the effect of the two methyltransferase genes BcOMT03 and BcOMT33 in catalyzing the ibullamcandin to produce the irisflorentin is defined, and the blank of research on the biosynthetic pathway of the characteristic component irisflorentin of the blackberry lily is filled up; important information is provided for research on a biosynthesis mechanism of active ingredients of medicinal plants.

This invention relates to elastomer compositions, elastomer compounds for tires, and vehicle wheel tires comprising said elastomer compounds, said elastomer compositions comprising a novel secondary crosslinking system comprising at least one methylene donor reagent having general formula (I) and at least one phenolic resin as a methylene acceptor reagent, wherein R1 is ethyl. The novel secondary crosslinking system of this invention allows for the imparting of optimal stiffness, thermal stability, tensile strength, and adhesion to reinforcing elements to materials, thereby improving the crosslinking kinetics and processability of the compound, while reducing or maintaining hysteresis. These materials comprising such crosslinking systems are less harmful than conventional crosslinking systems because they do not contain resorcinol, formaldehyde, and similar substances, and are therefore advantageously suited for tire components requiring a certain stiffness and tensile strength and / or high adhesion to fabric or metal reinforcing elements and low rolling resistance. (I)

The invention relates to application of S-adenosylmethionine or salt thereof in preparation of a medicine for preventing and treating ATO cardiotoxicity, and belongs to the field of biological medicine. In order to solve the problem that a general ferroptosis inhibitor cannot prevent and treat ATO cardiotoxicity from the source, the invention provides application of S-adenosylmethionine or salt thereof in preparation of drugs for preventing and treating ATO cardiotoxicity. The invention discloses a key molecular pathway of cardiac toxicity caused by ATO: ATO methylation metabolism exhausts endogenous methyl donors of myocardial cells, causes methylation imbalance of histone H3K9me3, and causes abnormal high expression of ferroptosis key protein HO-1 and mitochondrial iron overload. On the basis, the invention creatively provides a targeted intervention strategy of exogenous supplementation of S-adenosylmethionine, methylation imbalance and ferroptosis pathway activation are blocked from the upstream, root prevention and treatment of ATO cardiotoxicity are realized, and cardiac insufficiency is improved. The targeted intervention strategy is good in safety and has the advantages of clinical transformation and long-term application.

The invention relates to a rubber composition based on at least one diene elastomer, a reinforcing filler, a crosslinking system, a lignin-based resin and a methylene donor-type hardener, the hardener being hexamethylenetetramine, denoted HMT, the mass ratio of HMT to lignin being greater than or equal to 0.15.

The invention discloses application of a PLAC8 promoter methylation promoter in preparation of a product for promoting angiogenesis of a pig placenta, and belongs to the technical field of biological medicines. Experiments prove that PLAC8 as an anti-angiogenesis factor can directly inhibit the angiogenesis function, and DNMT3A can promote placenta vascular development and improve sow farrowing performance by regulating the methylation level of a PLAC8 promoter. In addition, it is determined that daily ration supplementation of methyl donors in the pig placenta vascular development peak period is an important nutrition way for improving DNA methylation of the PLAC8 promoter region. According to the method, the placenta injury mechanism under the adverse pregnancy outcome is directly determined through combination of in-vivo and in-vitro experiments, a theoretical basis is provided for analysis of a regulation strategy for promoting pig placenta angiogenesis and improving sow reproductive performance from epigenetics, and the accuracy and credibility of a result are improved.

An environmentally friendlier rubber composition is provided that is comprised of a phenolic resin and a phenolic epoxide having adequate performance characteristics and, wherein no methylene donors are utilized that generate formaldehyde during the curing of the rubber resulting in a rubber composition that has little or no formaldehyde.

The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.

The invention discloses a method for synthesizing ergothioneine based on a chemical-enzyme method, and belongs to the technical field of biology. The method comprises the following steps: firstly, synthesizing histidine betaine by using a chemical method, and carrying out selective trimethylation reaction in an alkaline aqueous solution by using methyl iodide as a methyl donor, so that the final concentration of histidine betaine is 77g / L, and the conversion rate is 93%; then, histidine betaine sulphide enzyme EanB from an anaerobic biological argillobiosis source is selected, a histidine betaine sulphide enzyme mutant F97A / T387M / E277K / N354Q / F441S / S335K is constructed through truncation transformation and mutation, and histidine betaine is directly vulcanized to synthesize ergothioneine. According to the present invention, through the cascade reaction of the chemical-enzyme method, the synthesis from the histidine to the ergothioneine is successfully achieved, the final ergothioneine yield is 33.5 g / L, and the conversion rate is 95%;

The invention discloses a process for synthesizing methylated flavonoids derivatives by utilizing a photocatalysis technology, which comprises the following steps: firstly, placing flavonoids derivatives in a glass test tube, then adding dimethyl sulfoxide (DMSO) as a methyl donor and a solvent, and adopting eosin Y as a photocatalyst to synthesize the methylated flavonoids derivatives. And 1-azabicyclo [2.2. 2] octane (quinuclidine) is added as an alkaline additive. Under the condition of room temperature, a blue light source with the power being 18 watts and the wavelength being 455 nanometers is used for carrying out illumination stirring reaction. And separating and purifying the crude product to obtain the 3-methyl flavonoid derivative. The invention realizes a photochemical C-H alkylation reaction of dimethyl sulfoxide and flavonoid derivatives, a series of functionalized methylated flavonoid derivatives can be constructed through simple operation, and compared with the traditional synthesis method, the method has the main advantages that: 1) the reaction condition is mild, and the reaction can be smoothly carried out at room temperature; the method has the advantages of simple operation, no need of metal catalysts and strong oxidants, no need of strong acids, simple operation, no need of nitrogen protection, high yield and good tolerance of functional groups.

The present invention relates to compositions comprising a methyl group donor compound and an acetyl-CoA donor compound for use in therapy and prevention of diseases associated with dysfunction of mitochondriae, wherein the diseases associated with dysfunction of mitochondriae are nucleotide excision repair deficiency syndromes, especially Xeroderma pigmentosum A, or DNA repair deficiency syndrome ataxia telangiectasia associated with mitochondrial dysfunction. Particularly, the composition is used for therapy of nucleotide excision repair deficiency syndromes, such as Xeroderma pigmentosum A, B, C, D, E, F, G, V, the different forms of Cockayne syndrome (CS), such as Cockayne syndrome type B (CSB) and Cockayne syndrome type A (CSA), and trichothiodystrophy (TTD), or DNA repair deficiency syndrome ataxia telangiectasia associated with mitochondrial dysfunction.

The invention discloses a method for producing vitamin B12 by dynamically regulating and controlling a pH value in a fermentation process, and belongs to the technical field of biochemistry and fermentation. Specifically, pseudomonas is adopted as a production strain of vitamin B12, beet molasses or glucose is adopted as a carbon source of a fermentation medium, and betaine or choline chloride is adopted as a methyl donor; and the yield of the vitamin B12 is improved by maintaining the stability of the pH value of the fermentation liquor. Industrial fermentation is carried out in a 120m fermentation tank, the pH value is dynamically monitored and regulated, the pH value is stably maintained in a range of 7.15-7.30, the total sugar concentration is kept at 30-40g / L in the fermentation process, the continuous feeding rate of methyl donor betaine is 0.036 m / h, the fermentation lasts from 45 hours to 140 hours, and the final yield of vitamin B12 reaches 214.3 mu g / mL, so that the production efficiency is remarkably improved, and the production cost is reduced. And the high-efficiency production of the vitamin B12 is realized in the industrial fermentation process.

The invention discloses a method for synthesizing methylated products of nitrogen heterocyclic and oxygen heterocyclic compounds, which comprises the following synthesis processes: firstly, placing a target heterocyclic compound in a glass reaction container, then adding dimethyl sulfoxide (DMSO) as a methyl donor and a solvent, adopting fluorescein as a photocatalyst, adding 1, 2, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4 2, 4-diazabicyclo [2.2. 2] octane (DABCO) is used as an alkaline additive. Under the condition of room temperature, a blue light source with the power being 10 watts and the wavelength being 455 nanometers is used for carrying out illumination stirring reaction. And after the reaction is completed, purifying through a column chromatography separation technology to finally obtain the methyl-substituted nitrogen heterocyclic or oxygen heterocyclic compound. The unique photocatalytic system disclosed by the invention can efficiently realize methylation reaction of nitrogen heterocyclic ring and oxygen heterocyclic ring without depending on a metal catalyst or a strong oxidant, and excellent yield and good functional group compatibility are obtained.

The invention provides a recombinant engineering bacterium for synergistically co-producing flavone 7-O-glucoside and application of the recombinant engineering bacterium, the recombinant engineering bacterium takes saccharomyces cerevisiae SQ05 as an original strain, exogenous expression of a Pc4CL gene, an SjCHS1 gene and an MsCHI gene is realized, the gene expression quantity of a naringenin synthetic route is increased, supply of coumaric acid and cytoplasmic acetyl coenzyme is increased, and efficient synthesis of naringenin is realized. And then, exogenous MpOMT, AtGT, Cm1, 2-RhaT and OIRHM genes are inserted, so that the coordinated co-production of the flavone 7-O-glucoside is realized. Further, the synthesis proportion of isosakuranetin 7-O-glucoside and naringenin 7-O-glucoside is adjusted by increasing the expression quantity of methyltransferase and enhancing the supply of a methyl donor and a glycosyl donor. The total yield of the target product is improved through a one-bacterium productive mode, and the difficulty and cost of a downstream purification process are remarkably reduced.

The present invention is directed to a method for shortening lag phase of a microorganism, including contacting the microorganism with an effective amount of a methyl group donor and / or one-carbon group donor, thereby shortening the lag phase of the microorganism.

This invention belongs to the field of enzyme engineering technology, specifically relating to proteins with tetrahydrofolate-dependent demethylase activity and their application in the synthesis of L-5-methyltetrahydrofolate. This invention utilizes two proteins with tetrahydrofolate-dependent demethylase activity, namely *Sphingosine monocytogenes* (…). Sphingomonas paucimobilis Vanillic acid O-demethylase and Amorpha fruticosa intermediate rhizobia (from) Mesorhizobium amorphae The aminomethyltransferase derived from 6S-THF uses inexpensive vanillic acid or dicamba as methyl donors to directly methylate 6S-THF to synthesize L-5-MTHF. Combined with highly active dihydrofolate reductase and NADP cofactor regeneration system, this method effectively achieves efficient and green synthesis of L-5-methyltetrahydrofolate, and has good industrialization prospects.

The application discloses a preparation method and application of S-adenosyl-methyl cysteine, and the preparation method comprises the following reaction: S-adenosyl-L-cysteine is methylated by using a first methyltransferase or a first methyltransferase mutant and a methyl donor to obtain S-adenosyl-methyl cysteine; or S-adenosyl-L-homocysteine is methylated by using a methyltransferase or a first methyltransferase mutant and a methyl donor to obtain S-adenosyl-methionine. The method has the characteristics of low cost and simple operation, and enables S-adenosyl-methyl cysteine to replace expensive S-adenosyl methionine (SAM) as a coenzyme and a methyl donor in a methylation reaction, and is applied to methylation modification of natural products based on a coenzyme regeneration system.

The invention discloses a preparation method of ergothioneine, which comprises the following steps: adding L-histidine and dimethyl carbonate into a solvent, carrying out oil bath condensation reflux reaction under an alkaline condition to obtain an L-histidine betaine crude liquid, and carrying out vacuum distillation on the L-histidine betaine crude liquid to obtain the ergothioneine. And adding water to a constant volume, adding tNcEgt1 wet thallus, NcEgt2 wet thallus, PLP, FeSO4. 7H2O and Cys into the solution with the constant volume, and reacting to obtain ergothioneine. In the first step, dimethyl carbonate is adopted as a methyl donor to replace expensive adenosylmethionine SAM, after the reaction in the first step is finished, the ergothioneine can be synthesized through catalytic conversion under the condition of high substrate concentration by directly adding an enzyme without crystallization, and the ergothioneine synthetase Egt1 is subjected to truncated expression by combining protein engineering, so that the expression quantity is improved, and meanwhile, the yield of the ergothioneine synthetase Egt1 is increased. The catalytic efficiency is improved, and the conversion efficiency of in-vitro catalysis of 22.5 g / L substrate concentration, 28 DEG C and 24 h is 99% or above.

This invention discloses the application of DNA methyl donors in the preparation of drugs for treating severe acute pancreatitis and / or its complications, as well as a method for screening candidate drugs. The application of DNA methyl donors provided by this invention in the preparation of drugs for treating severe acute pancreatitis and / or its complications, by supplementing the methyl donor, restores the methylation level of the Foxp1 enhancer, blocks the "FOXP1-CXCR4" axis upstream, significantly reduces pancreatic microthrombus formation and alleviates tissue necrosis, and avoids the systemic side effects of directly antagonizing CXCR4.

The invention discloses a synthetic method of fluoromethane, which comprises the following steps: (1) taking trifluoromethanesulfonic anhydride and methanol as raw materials, and synthesizing methyl trifluoromethanesulfonate through esterification reaction; and (2) taking methyl trifluoromethanesulfonate as a methyl donor, and carrying out nucleophilic substitution reaction on potassium fluoride under the assistance of phase transfer agents such as crown ether / PEG (Polyethylene Glycol) to generate fluoromethane. The reaction condition is mild, esterification is completed at normal temperature and normal pressure, and the high toxicity risk of a traditional dimethyl sulfate method is avoided. The conversion rate is high, the activity of trifluoromethanesulfonic anhydride is high, excessive methanol ensures complete reaction, and the esterification efficiency is not less than 95%. The invention provides a novel process for synthesizing fluoromethane by taking methyl trifluoromethanesulfonate as a methyl donor and potassium fluoride as a fluorine source, and the novel process has the technical significance of low cost, low energy consumption and controllable equipment, and is suitable for commercial amplification.