23 results about "Molecular pathway" patented technology

The invention discloses an application of a targeted ECT2 inhibitor in preparation of a product for treating renal interstitial fibrosis. According to the application, a brand-new and unprecedented molecular pathway which activates a p53 pathway by inhibiting ribosome biogenesis (RiBi) and finally causes renal tubular cell aging and renal fibrosis is revealed and confirmed for the first time, and a solid theoretical basis is provided for precise drugs aiming at the pathway; and a major breakthrough from relevance observation to causality mechanism clarification is realized.

The present invention relates to a pharmaceutical composition for use in the treatment of both low-grade and high-grade glioblastoma in a human subject. The composition comprises a synergistic combination of the following active agents: R-Ketorolac, Bumetanide, and Niflumic acid. This specific combination is designed to exert a synergistic therapeutic effect against glioblastoma by targeting multiple molecular pathways involved in tumor progression, inflammation, and ion transport. In a further aspect of the invention, there is provided a method for selecting a subpopulation of glioblastoma subjects who are responsive to the aforementioned treatment. This method comprises identifying genetic profiles predictive of a favorable therapeutic response to the combined administration of R-Ketorolac, Bumetanide, and Niflumic acid.

The invention belongs to the technical field of transgenic and biomedical research tools, and particularly relates to a method for constructing a transgenic fish system based on a Tol2 transposon system, which comprises the following steps: constructing a myo6b-lhfpl5b-DsRed plasmid by using myo6b as a promoter, lhfpl5b as a target gene and DsRed as a red fluorescent protein tag through a microinjection method, and constructing a Tol2 transposon system-based transgenic fish system. Co-injecting the plasmid containing the Tol2 transposase mRNA and the Tol2 transposase mRNA into a fertilized egg of an AB zebra fish strain, and screening generation by generation to obtain a transgenic fish strain with stable inheritance MET channel gene lhfpl5b marked hair cells. The spatio-temporal dynamic visualization of the living whole fish to the lhfpl5b is realized, the deafness-causing molecular pathway is clarified, and an in-vivo platform is provided for drug screening and intervention strategies.

The invention relates to application of S-adenosylmethionine or salt thereof in preparation of a medicine for preventing and treating ATO cardiotoxicity, and belongs to the field of biological medicine. In order to solve the problem that a general ferroptosis inhibitor cannot prevent and treat ATO cardiotoxicity from the source, the invention provides application of S-adenosylmethionine or salt thereof in preparation of drugs for preventing and treating ATO cardiotoxicity. The invention discloses a key molecular pathway of cardiac toxicity caused by ATO: ATO methylation metabolism exhausts endogenous methyl donors of myocardial cells, causes methylation imbalance of histone H3K9me3, and causes abnormal high expression of ferroptosis key protein HO-1 and mitochondrial iron overload. On the basis, the invention creatively provides a targeted intervention strategy of exogenous supplementation of S-adenosylmethionine, methylation imbalance and ferroptosis pathway activation are blocked from the upstream, root prevention and treatment of ATO cardiotoxicity are realized, and cardiac insufficiency is improved. The targeted intervention strategy is good in safety and has the advantages of clinical transformation and long-term application.

The invention relates to a medicine for preventing and treating cerebral cavernous malformation lesion and application thereof. The invention discloses a medicine which can inhibit the occurrence and growth of a cerebral cavernous malformation focus by inhibiting a molecular pathway of CCM, and also comprises analogues or derivatives of the medicine. The treatment of the CCM generally takes the brain as a drug target, the lower toxic and side effects particularly need to be concerned, and the drug disclosed by the invention has low side effects. The medicine provided by the invention can limit the occurrence of lesions in the initial stage of CCM, and is also suitable for preventing the progress of advanced lesions.

Disclosed herein are methods of and compositions for identifying components of a molecular pathway, wherein the molecular pathway comprises a cell surface receptor. The method comprises use of ligand-coated nanoparticles and cells that internalize the ligand-coated nanoparticles. The methods allow analysis of a wide range of ligand- receptor complexes.

The invention discloses a ZmCUC2 gene, a corn plant type and ear character regulation and control method based on the gene and application, and aims to solve the technical problem that in the prior art, an effective molecular target and a technical means for regulating and controlling synergistic improvement of corn plant height and ear characters are lacked. The invention discloses a method for improving agronomic traits of corn by regulating and controlling expression and / or activity of a corn ZmCUC2 gene, and discloses that ZmCUC2 regulates and controls a molecular pathway of ear development by directly combining and activating a downstream F-box gene promoter for the first time. The method has the beneficial effects that the new corn germplasm with increased plant height, remarkably increased ear length and ear thickness and increased yield is created, and a new target and a general technical scheme are provided for high-yield molecular breeding of the corn.

The invention discloses a bacterial infection and LPS induced broiler chicken liver injury model and a construction method and application thereof, and the construction method comprises the following steps: administering 10-day-old broiler chicken with Escherichia coli E.coli O78 with a preset concentration in an intragastric administration manner; after broiler chickens are subjected to intragastric administration of escherichia coli for 18 hours, an LPS solution is injected into the intraperitoneal cavity of the broiler chickens, after the LPS solution is injected for 24 hours, the broiler chicken liver injury model is obtained, when the broiler chickens are 11 days old, the broiler chickens subjected to intragastric administration of escherichia coli are subjected to intraperitoneal injection of the LPS solution, and after the LPS solution is injected for 24 hours, the broiler chicken liver injury model is obtained. The broiler chicken liver injury model is constructed by intragastric administration of avian pathogenic escherichia coli (E.coli O78) and intraperitoneal injection of LPS (2 mg / kg), the pathology of the model is highly consistent with that of clinical broiler chicken bacterial liver injury, the liver has clinical typical lesions macroscopically, liver cells are degenerative and necrotic microscopically, and a molecular pathway activation mode is consistent with that of clinical application; the model constructed by the invention realizes specific liver injury, only the liver has specific injury, multiple organ lesions such as heart, peritoneum and the like do not exist, and the model availability is high.

The invention relates to the technical field of biological medicine, in particular to application of TRIM21 serving as a biomarker in screening or preparing a preparation for reducing ciprofloxacin exposure related abortion risk. The problems that in the prior art, key action links and molecular mechanisms of pregnancy loss caused by exposure of environment or non-therapeutic dose ciprofloxacin are not clear, and objective detection markers capable of prompting risk states in the early stage of pregnancy and relatively standard and repeatable detection methods are lacked are solved. The invention discloses an application of TRIM21 as a biomarker in screening or preparing a preparation for reducing miscarriage risk related to ciprofloxacin exposure, the pregnancy risk caused by ciprofloxacin exposure can be recognized in an early stage by detecting the expression level of TRIM21, and a candidate preparation with a protective effect is further screened. The invention provides a new thought and target for developing a novel medicine for preventing abortion.

The invention provides a construction method of an embryo-like model, which comprises the following steps: culturing primitive state stem cells in vitro or injecting the primitive state stem cells into an immunodeficient animal body for differentiation to obtain the embryo-like model. According to the method, in the process of preparing the embryo-like model, cell factors and small molecular pathway interference factors do not need to be added, gene editing is not needed, only a single starting cell type is adopted, and the method has the advantages of being simple and high in repeatability, facilitates large-scale preparation and application, and has wide application prospects. And the prepared embryo-like model has all embryo external cell lineages and all embryo internal cell lineages except for trophoderm, and can simulate the development process from an implanted embryo of a Carbonate-resistant group 3 stage to a Carbonate-resistant group 9 stage nerve embryo, so that the embryo-like model can be used for preparing various progenitor cell lineages, modeling diseases, screening drugs and carrying out scientific research.

This invention discloses a pharmaceutical composition and its application in the preparation of drugs for treating CSVD, belonging to the field of biomedical technology. The pharmaceutical composition comprises at least one GLP1R agonist and at least one VEGF-A inhibitor. Through Mendelian randomization studies, this invention discovered and revealed that the protective effect of GLP1R agonists in alleviating WMH is partly mediated by downregulating the specific molecular pathway of vascular endothelial growth factor (VEGF-A), indicating that VEGF-A is not only a pathogenic factor of CSVD but also a key downstream node for the therapeutic effect of GLP1R agonists. Based on the above findings, animal model experiments confirmed that compared to traditional single-target monotherapy, combining GLP1R agonists and VEGF-A inhibitors can synergistically enhance the effects and achieve more comprehensive and precise intervention and treatment of CSVD.

The invention belongs to the technical field of medicines, and discloses naringenin for inhibiting colorectal cancer progression through a targeted Gankyrin / PI3K / AKT / GSK-3beta / beta-catenin signal channel. The invention discloses an application of naringenin in preparation of a medicine for preventing or treating colorectal cancer. The treatment potential of NAR and related molecular pathways thereof in colorectal cancer CRC is evaluated through a bioinformatics method. Subsequently, the anti-CRC characteristic and the molecular mechanism of the NAR are verified in in-vivo and in-vitro experiments by adopting a gene interference technology and a molecular biology means. Research results show that the NAR inhibits CRC cell proliferation, migration and invasion ability and promotes cell apoptosis by regulating and controlling a Gankyrin / PI3K / AKT / GSK-3beta / beta-catenin signal channel, and the NAR has great potential as a medicine for treating colorectal cancer.

The invention relates to the technical field of medical images, in particular to a method for predicting liver cancer TACE treatment prognosis through immune infiltration related image group scoring based on transfer learning, which comprises the following steps of: acquiring a preoperative MRI image and constructing a pattern label, extracting an evolution feature block, establishing a co-occurrence relation between the image and immune expression, and establishing a TACE treatment prognosis model according to the co-occurrence relation between the image and the immune expression. The fusion gene and the pathway sequence form a splicing structure, and a prognosis distribution diagram is generated by mapping layer levels. According to the method, pattern labels are constructed based on image gray and boundary directions, evolution blocks are formed in combination with signal trends and structural stability, spatial mapping is established by using block numbers and immune expression positions, expression trends and gene identifiers are fused to construct a path aggregation structure, and survival levels are mapped according to a layer sequence. Cooperative association of image features, immune response and molecular pathways in the spatial dimension is achieved, and the focus structure recognition definition, the expression position corresponding precision and the prognosis prediction stability under multi-layer information linkage are improved.

The invention relates to application of hordenine in preparation of a medicine for treating attention deficit hyperactivity disorder, and belongs to the technical field of biological medicine. According to the invention, 1119 traditional Chinese medicine monomers are subjected to primary screening, secondary screening and dose-dependent verification by using a per1b knockout zebrafish ADHD model, and hordenine is found to be a compound capable of remarkably rescuing ADHD-related behavior abnormalities, and after the hordenine is treated at the optimal concentration of 30 mu M for 1 hour, the hordenine can be used for remarkably rescuing ADHD-related behavior abnormalities. The hordenine remarkably saves excessive movement, impulse and inattention of the per1b <- / -> zebra fish model. On the molecular level, hordenine can restore maladjustment neurotransmitter receptor gene expression to a wild type level and regulate key molecular pathways involved in synapse and neurotransmitter signal transduction. The discovery shows that hordenine has a stable and repeatable behavior rescue effect, and supports the potential of hordenine as an anti-ADHD potential drug.

The invention relates to a library construction method based on high-throughput sequencing endometrial cancer molecular typing, and relates to the technical field of medical data process.The library construction method includes the steps that a clinical index-molecular feature linked dynamic typing system is established, and sequencing data of peripheral blood LDL concentration and other clinical metabolic indexes and tumor tissue pathway genes are integrated; a multi-dimensional data analysis model is established, double typing of metabolic phenotypes and molecular pathways of endometrial cancer is realized, a basis is provided for matching differentiated treatment schemes for patients with different subtypes, the typing specificity and accuracy are improved, a high-throughput sequencing library of targeted LDL metabolism and JAK / STAT pathway genes is constructed, and a high-throughput sequencing library of targeted LDL metabolism and JAK / STAT pathway genes is established. Nucleic acid fragments of key genes such as JAK2, STAT3 and LDL receptors are enriched through a specific probe, irrelevant nucleic acid interference is eliminated, the detection sensitivity and typing accuracy of LDL-JAK / STAT pathway abnormal endometrial cancer are improved, and the technical blank of special subtype molecular typing is filled.

This invention discloses tomatoes SlARP6 The application of genes as negative regulators in improving the cold tolerance of tomatoes, the aforementioned SlARP6 The nucleotide sequence of the protein-coding region of the gene is shown in SEQ ID NO: 1, and the application method is through knockout. SlARP6 The gene significantly enhances the low-temperature resistance of tomatoes. This invention provides key gene resources for the breeding of new low-temperature resistant tomato varieties, clarifying... SlARP6 This study explores the application value of genes as negative regulators of low-temperature resistance; it also lays an important theoretical foundation for elucidating the molecular pathways of tomato's response to stress signals and the molecular mechanisms of tolerance to adverse environments, and provides new ideas for molecular breeding practices to improve plant stress resistance.

The invention relates to an accelerant for up-regulating key genes of a steroid hormone synthesis pathway of scapharca broughtonii and application of the accelerant, and belongs to the technical field of aquaculture, the accelerant is prepared from the following components in parts by mass: 15-20 parts of cholesterol, 8-10 parts of linolenic acid, 10-12 parts of phosphatidylcholine, 0.5-0.8 part of zinc sulfate, 30-40 parts of L-arginine and 0.5-0.75 * 10 <-3 > part of vitamin D3. Core genes (SF-1, StAR, 17 beta-HSD14 and CYP17) of a steroid hormone synthesis pathway of scapharca broughtonii are specifically focused, synergistic up-regulation of the genes is realized through a gonad maturation accelerator, a core molecular pathway of gonad development is directly activated, and the regulation effect is accurate and stable.

This invention discloses a material-empowered stem cell-derived exosome, its preparation method, and its application, belonging to the field of biomedical technology. The method involves the following steps: mesenchymal stem cells are seeded onto a substrate fused with N / VE-cadherin-Fc fusion protein and cultured to assemble into aggregates; the aggregates are dynamically cultured under staged shear stress; the culture medium is collected, and the exosomes are separated and purified. The preparation method of this invention overcomes the shortcomings of existing mesenchymal stem cell exosome preparation technologies, such as low yield and insufficient neurovascular repair function. It significantly activates exosome biogenesis-related molecular pathways, increases exosome yield by approximately four times, and is rich in miRNAs related to neurovascular repair. It can significantly improve neurological deficits in stroke mice and hindlimb motor function in rats with spinal cord injury, providing a highly efficient and stable cell-free therapeutic strategy for the treatment of central nervous system injuries.

A computer-implemented method for the design of immunofluorescence cellular research tests through a smart assistant in a graphical user interface. The methods include receiving a user-defined molecular pathway and generating an interactive pathway map with functional protein data and assay tool access. The assistant retrieves assay function data containing antibody and fluorophore data, including host species, cross-reactivity, and spectral properties. The user selects and the system displays an assay guide with filtered antibody options for selected proteins and a dye selection guide based on wavelength input. The assistant evaluates fluorophore compatibility with the imaging system using predictive modeling. Finally, it generates a tailored molecular experiment protocol, including selected proteins, antibodies, fluorophores, and optimized testing protocols, each with a confidence score derived from machine learning models trained on historical data.

A computer-implemented method for the design of immunofluorescence cellular research tests through a smart assistant in a graphical user interface. The methods include receiving a user-defined molecular pathway and generating an interactive pathway map with functional protein data and assay tool access. The assistant retrieves assay function data containing antibody and fluorophore data, including host species, cross-reactivity, and spectral properties. The user selects and the system displays an assay guide with filtered antibody options for selected proteins and a dye selection guide based on wavelength input. The assistant evaluates fluorophore compatibility with the imaging system using predictive modeling. Finally, it generates a tailored molecular experiment protocol, including selected proteins, antibodies, fluorophores, and optimized testing protocols, each with a confidence score derived from machine learning models trained on historical data.

The invention provides a multi-dimensional evaluation method for insulin-resistant hepatocytes, which specifically comprises the following steps: performing phenotype screening on an insulin-resistant hepatocyte model, constructing a cell model and setting experimental groups; the influence on an insulin signal channel is verified through western blot; and finally performing comprehensive evaluation: when the phenotypic index is not matched with the mechanism index, further verifying the inspection range of time gradient, mitochondrial dynamics related protein or insulin signal pathway key protein so as to determine the subsequent research direction, and the method realizes in-situ correlation analysis of morphology, function and molecular pathways, is comprehensive in evaluation dimension, and is suitable for large-scale popularization and application. And in-depth research can be efficiently guided.

The application belongs to the technical field of animal models, and provides a method for constructing a mouse testis tissue stress granule animal model, which comprises the following steps: after the mouse is anesthetized, the testis and epididymis of one side of the mouse are taken out; a sodium arsenite solution containing fast green dye is injected into the testis of the mouse through the entrance of the rete testis, and the concentration of the sodium arsenite solution is 30-50 mM; the wound of the mouse is sutured, the mouse is killed after the mouse recovers for 3 hours, and the testis tissue is separated for an immunofluorescence experiment; when obvious stress granules are formed, it is indicated that the model construction is successful. The application also provides an application of the method for constructing an animal model in identifying the generation of stress granules of spermatocytes and in identifying the generation of RNA binding protein SERBP1. The application provides a method for exploring the role of SERBP1 protein in the stress granules of the mouse testis tissue, a related mechanism and a possible molecular pathway, and provides a new theoretical basis for guiding the generation and recovery of cell stress granules under special conditions.