Systemic lupus erythematosus lesion immune abnormality detection method based on circRNA

A lupus erythematosus and immune abnormality technology, applied in the fields of immunity and molecular biological detection, can solve problems such as difficulty, unclear diagnostic markers and treatment markers for the pathogenesis of lupus erythematosus, and difficulty in finding regulatory target operations.

Pending Publication Date: 2020-07-07
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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Problems solved by technology

Further technical problems: it is difficult to fundamentally find the target to regulate the occurrence of the disease, and the operation is difficult, and the controllability is poor, etc.
[0006] In summary, the existing problems in the prior art are: the pathogenesis, diagnostic markers and therapeuti

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  • Systemic lupus erythematosus lesion immune abnormality detection method based on circRNA
  • Systemic lupus erythematosus lesion immune abnormality detection method based on circRNA
  • Systemic lupus erythematosus lesion immune abnormality detection method based on circRNA

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[0057] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0058] In view of the problems existing in the prior art, the present invention provides a method for detecting immune abnormalities of circRNA in systemic lupus erythematosus lesions. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0059] like figure 1 As shown, the circRNA provided by the embodiments of the present invention includes the following steps in the systemic lupus erythematosus lesion immune abnormality detection method:

[0060] S101: Collect PBMCs samples of peripheral blood mononuclear cells, and use RNA-seq sequencing technology to conduct a comprehensi...

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Abstract

The invention belongs to the immunity and molecular biological detection technology field, and discloses a systemic lupus erythematosus lesion immune abnormality detection method based on circRNA. Themethod comprises the steps of collecting peripheral blood mononuclear cell PBMCs specimen, and carrying out detection analysis on gene expression through an RNA-seq sequencing technology; picking out20 genes with significant difference; based on circRNA involved transcription post regulation, performing matching analysis on candidate genes, and screening out miRNA with targeted combination; andby performing summarizing on the targeted combined miRNA, screening out miRNA with representative research value from SLE. From cell and molecular levels, the mode of action and molecular mechanism inlymphocyte immune balance abnormal regulation SLE pathogenesis can be further researched deeply, the mediated signal pathway is explicit and the molecular pathway for developing functional effects ofcircRNA is determined.

Description

technical field [0001] The invention belongs to the technical field of immunity and molecular biology detection, and in particular relates to a method for detecting abnormal immunity of circRNA in systemic lupus erythematosus lesions. Background technique [0002] Currently, closest available technology: Immunological tests such as anti-dsDNA antibodies, complement activity, and immune complexes have long been classic technical markers of SLE disease activity. However, the main obstacles are 1. The related genes and expression products are also related to other autoimmune diseases, and the specificity is not high; 2. The body changes slightly in the early stage of SLE, and the amount of related proteins produced by the lesion is very small, or even cannot appear; There are large differences between. This technology starts from the etiological genetic factors and combines RNA-seq high-throughput detection methods to find disease-specific genetic variables, and explores the w...

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Application Information

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IPC IPC(8): C12Q1/6883G16B20/00
CPCC12Q1/6883G16B20/00C12Q2600/158C12Q2600/178
Inventor 赵兴旺倪兵游弋郭俊恺张龙龙葛兰王娟王春又赵承磊刘文英吴奇俊高翠娥宋志强
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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