Systemic lupus erythematosus lesion immune abnormality detection method based on circRNA

Abstract

The invention belongs to the immunity and molecular biological detection technology field, and discloses a systemic lupus erythematosus lesion immune abnormality detection method based on circRNA. Themethod comprises the steps of collecting peripheral blood mononuclear cell PBMCs specimen, and carrying out detection analysis on gene expression through an RNA-seq sequencing technology; picking out20 genes with significant difference; based on circRNA involved transcription post regulation, performing matching analysis on candidate genes, and screening out miRNA with targeted combination; andby performing summarizing on the targeted combined miRNA, screening out miRNA with representative research value from SLE. From cell and molecular levels, the mode of action and molecular mechanism inlymphocyte immune balance abnormal regulation SLE pathogenesis can be further researched deeply, the mediated signal pathway is explicit and the molecular pathway for developing functional effects ofcircRNA is determined.

Description

technical field [0001] The invention belongs to the technical field of immunity and molecular biology detection, and in particular relates to a method for detecting abnormal immunity of circRNA in systemic lupus erythematosus lesions. Background technique [0002] Currently, closest available technology: Immunological tests such as anti-dsDNA antibodies, complement activity, and immune complexes have long been classic technical markers of SLE disease activity. However, the main obstacles are 1. The related genes and expression products are also related to other autoimmune diseases, and the specificity is not high; 2. The body changes slightly in the early stage of SLE, and the amount of related proteins produced by the lesion is very small, or even cannot appear; There are large differences between. This technology starts from the etiological genetic factors and combines RNA-seq high-throughput detection methods to find disease-specific genetic variables, and explores the w...

AI Technical Summary

Problems solved by technology

Further technical problems: it is difficult to fundamentally find the target to regulate the occurrence of the disease, and the operation is difficult, and the controllability is poor, etc.

[0006] In summary, the existing problems in the prior art are: the pathogenesis, diagnostic markers and therapeutic markers of the existing systemic lupus erythematosus are unclear.

[0007] The difficulty of solving the above technical problems: it is difficult to fundamentally find the target to regulate the occurrence of the disease and the operation is difficult, and the controllability of variables is poor, etc.

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