The invention relates to a rabbit hemorrhagic disease virus(RHDV) colloidal gold test strip, and belongs to the technical field of immune tests of the colloidal gold. One end of a bottom plate of the test strip is a colloidal gold-RHDV polyclonal antibody combination glass fiber film, and an antibody solid-phase cellulose nitrate film is arranged on the middle part of the bottom plate; a RHDV single-resistance test line, namely T line and a goat anti-rabbit IgG control line, namely C line are arranged on the bottom plate; and the other end of the bottom plate is absorbent paper. When a tested sample is carried with the RHDV, the RHDV can specifically combine the anti-RHDV polyclonal antibody labeled by the colloid so as to be identified by the anti-RHDV monoclonal antibody, namely the double-antibody sandwich specific combination happens, so that whether the sample is carried with the RHDV can be tested. The invention also provides a RHDV immunological test method established by utilizing the monoclonal antibody and the polyclonal antibody, which has the advantages of quickness, accuracy, convenience and the like, and the method is particularly suitable for the on-the-spot test and preliminary screening test and has bright prospect.