5 results about "Saxitoxin" patented technology

The application relates to the technical field of aquatic virus prevention and treatment, and particularly discloses application of YTX in preparation of a medicine for preventing and treating SCRV infection. The application verifies the prevention and treatment effects of YTX on SCRV infection through fish body level experiments. The experimental results show that, in a safe concentration range, YTX can significantly improve the survival rate of fish after SCRV infection by being administered through soaking or injection, the relative protection rate is more than 85%; the virus load in target organs such as kidneys and spleens is significantly reduced, the virus copy number is reduced by 2-3 logarithmic levels; and the pathological damage such as tissue necrosis and inflammatory infiltration caused by the virus is effectively reduced. The application verifies the anti-SCRV effect of YTX at the fish body level for the first time, provides key data support for development and application of YTX as a new aquatic antiviral preparation, and has important industrial application value.

The invention discloses a rapid marine product toxin detection method and system, and relates to the technical field of food safety detection.The rapid marine product toxin detection method comprises the steps that 5-10 g of a marine product sample is taken and homogenized through a portable handheld homogenizer, then 8-15 mL of a composite extracting solution is added, extraction is conducted for 8-12 min in a portable ultrasonic instrument at 28-38 DEG C, centrifugation is conducted for 3-4 min through a portable mini centrifugal machine, and the marine product toxin is obtained; taking supernate as to-be-detected liquid; the method comprises the following steps: preparing a test strip which takes a nitrocellulose membrane as a substrate and contains 3-5 parallel detection areas, and fixing aptamer-quantum dot conjugates corresponding to saxitoxin in each detection area; dropwise adding the to-be-detected liquid to a sample pad of the test strip, reacting for 4-6 minutes at 25-35 DEG C, and scanning the fluorescence intensity by using a portable fluorescence reading instrument; and the analysis module compares the threshold value output result within 15 seconds and synchronizes the result to the mobile terminal through Bluetooth. The method is completed within 30 minutes in the whole process, equipment is portable, multiple toxins can be synchronously detected on site, the screening efficiency is improved, and the food safety risk is reduced.

The application discloses a kind of kaurane diterpene compounds or its medicinal salt, its chemical structure is as shown in formula (I). The compound is extracted, isolated from the fermentation product of the fungus (Eutypella sp.D-1) of the north polar arched shell genus (Eutypella sp.D-1) with the preservation number CCTCC NO:M 2013144 for the first time.The application further provides the extraction method of the compound, including the steps of liquid fermentation of fungus, extraction obtains total extract, and separation and purification by vacuum column chromatography, reverse phase ODS column chromatography and high performance liquid chromatography. Through zebra fish and mouse in-vivo pharmacological experiment, the compound of the application can significantly improve the abnormal behavior induced by domoic acid (DA), prolong the latent period of poisoning onset, relieve the symptoms of poisoning, and exhibit good neuroprotective activity. Therefore, the compound of the application can be used as a lead compound for preparing a drug for preventing or treating domoic acid poisoning and memory loss injury caused thereby, and provides a scientific basis for developing and utilizing polar marine medicinal resources.

The invention relates to the technical field of aquatic product quality safety, in particular to a saxitoxin risk formation chain characterization monitoring system, which comprises a data acquisition module, a data processing module, a data processing module and a display module, the risk chain association module is used for constructing a multi-factor toxin association mechanism model, analyzing and quantifying influence weights of environmental factors through a causal inference algorithm and a random forest weight, and identifying key regulation points and threshold values of toxin synthesis; the risk chain mechanism analysis module carries out deduction through a digital twin simulation platform and a dynamic Bayesian network, simulates the dynamic change of a toxin anabolism pathway under different environment scenes, and obtains a risk formation chain and a risk change rule thereof; and the risk monitoring and intervention module constructs a global risk prediction model to obtain a risk level, obtains an emergency scheme from the emergency decision library according to the risk level and the toxin type, and performs intervention according to the emergency scheme. Therefore, the problems of monitoring lag, unknown causality, low intervention efficiency and the like are solved.

The invention discloses a pimarane diterpenoid compound or a pharmaceutical salt thereof. The chemical structure of the pimarane diterpenoid compound is shown as a formula (I). The compound is extracted and separated from a fermentation product of Eutypella sp.D-1 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2013144 for the first time. The invention also provides an extraction method of the compound. The extraction method comprises the following steps: carrying out liquid fermentation on fungi, extracting to obtain a total extract, and carrying out separation and purification through reduced pressure column chromatography, reversed phase ODS column chromatography and high performance liquid chromatography. As proved by in-vivo pharmacological experiments of zebra fish and mice, the compound disclosed by the invention can be used for remarkably improving behavior abnormality induced by domoic acid (DA), prolonging the poisoning attack incubation period and relieving poisoning symptoms, and shows good neuroprotective activity. Therefore, the compound disclosed by the invention can be used as a lead compound and is used for preparing a medicine for preventing or treating domoacidosis and memory deficiency injury caused by domoacidosis, and a scientific basis is provided for developing and utilizing polar marine medicinal resources.