46 results about "Single amino acid" patented technology

The invention provides Cas12a mutants from Murraya bovis AAX08, and methods of using the Cas12a mutants. The Mb2Cas12a mutant comprises a single amino acid substitution, a plurality of amino acid substitutions, a structural domain exchange, or all of the above. Compared with a wild type moraxella bovine eye AAX08Cas12a enzyme, the mutants have enhanced DNA (Deoxyribose Nucleic Acid) lysis activity in plants.

The present disclosure relates to a codon optimization technique based on token classification. In a model training phase, a collected protein sequence is tokenized by using a single amino acid as a token, and a nucleic acid sequence encoding a protein and corresponding to the protein sequence is tokenized by using a single codon as a token; a plurality of different synonymous codons in the tokenized nucleic acid sequence encoding the protein and corresponding to a single amino acid in the tokenized protein sequence are sorted in a descending order of frequency of occurrence in an expression system of the protein, and a classification label is set, thereby training a natural language model. In a prediction phase of the nucleic acid sequence encoding the protein, a protein sequence to be subjected to codon optimization is tokenized. The tokenized protein sequence is input into the trained natural language model to predict a classification label of a synonymous codon corresponding to a single amino acid in the tokenized protein sequence. An optimized nucleic acid sequence is obtained on the basis of a mapping relationship between the synonymous codon and the classification label thereof.

The invention belongs to the technical field of gene engineering and cell transformation, and discloses a gene engineering construction method and application of anti-apoptosis human mesenchymal stem cells. Through HDAC1 K412 site specific lactic acid mutation, breakthrough improvements are realized: for example, CRISPR-Cas9-mediated accurate gene editing is utilized, only a single amino acid site is changed, that is, K412R mutation simulates persistent lactic acid, and whole genome apparent modification disturbance can be avoided; the modification does not depend on an exogenous metabolic substrate, and the negative feedback effect of traditional metabolic intervention is overcome; the mutant continuously inhibits a P53 apoptosis pathway, so that the oxidative stress mediated apoptosis resistance of the MSC is remarkably improved. According to the design concept of one target spot and one regulation, the core contradiction that long-term effectiveness and specificity cannot be considered in the prior art is fundamentally solved, and a brand new normal form is provided for stem cell treatment.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The invention discloses a method for detecting polypeptide sequence difference and post-translational modification thereof based on a nanopore system, the nanopore system is constructed in the method, and the nanopore system comprises an alpha-hemolysin nanopore and octa-amino modified gamma-cyclodextrin embedded in the nanopore; the amino acid sequence of the alpha-hemolysin nanopore comprises mutation sites M113R and T145R, and the alpha-hemolysin nanopore is characterized in that the alpha-hemolysin nanopore According to the detection method, polypeptide with negative charges enters a nanopore under the driving of external voltage, an ion current blocking signal is recorded, and the polypeptide sequence difference and post-translational modification are analyzed according to blocking amplitude, duration, noise characteristics and event frequency. The method can detect a peptide chain with the length of 2-10 amino acid residues, can distinguish the post-translational modification state in homopolypeptide composed of 4-10 amino acids and polypeptide by single amino acid resolution, and can be used for protein enzyme digestion product analysis, oligopeptide drug detection and rapid polypeptide identification under the condition of no mass spectrum.

The invention relates to the technical field of biology, in particular to a protective monoclonal antibody targeting BCG (bacillus calmette guerin) BCG3965 as well as a preparation method and application of the protective monoclonal antibody. The antibody or an antigen binding fragment comprises a CDR sequence selected from at least one of the following sequences or a sequence with one amino acid substituted, deleted or increased: a heavy chain variable region CDR sequence: SEQ ID NO: 3-5; and the light chain variable region CDR sequences are as shown in SEQ ID NO: 7-9. The monoclonal antibody 5F10 shows efficient antituberculous activity in vivo and in vitro through specific targeting of BCG3965 protein on the surface of mycobacterium tuberculosis, mainly including the aspects of remarkably enhancing the phagocytosis of macrophages on tubercle bacillus, inhibiting tubercle bacillus growth and the like, and in addition, the monoclonal antibody 5F10 is clear in sequence and structure, easy to develop and modify, and capable of being used for preparing antituberculous drugs for treating mycobacterium tuberculosis. The monoclonal antibody 5F10 disclosed by the invention has the advantages that the monoclonal antibody 5F10 is not easy to induce pathogens to generate drug resistance by an immune-mediated treatment mechanism, and the monoclonal antibody 5F10 is applied to prevention and treatment of tuberculosis, not only provides a new choice for treatment of tuberculosis, but also provides an important technical basis for response of drug-resistant strains, development of diagnostic reagents, vaccine development and the like, and is wide in application prospect.

The application discloses a protein post-translational modification prediction method based on multi-head attention, and belongs to the technical field of biological information, and comprises the following steps: preprocessing protein sequences with post-translational modification annotation information to obtain a legal character set, a second protein sequence data set and a benchmark data set; obtaining three kinds of vocabulary tables based on the legal character set; performing multi-scale division on protein sequence samples in the second protein sequence data set to obtain a single amino acid scale data set, a dipeptide scale data set and a tripeptide scale data set; training a corpus by using a Skip-gram model structure to obtain three kinds of word vector models; performing label prediction on protein sequence fragments of the benchmark data set by using a multi-head attention model to obtain protein sequence fragment prediction results; and the multi-scale word vector is used to comprehensively utilize polypeptide composition information in the original protein sequence, and the dependence on biological feature engineering is reduced.

Methods of generating a limb-girdle muscular dystrophy (LGMD) functional score, as well as methods of predicting and treating LGMD are provided. The present disclosure teaches methods of generating a LGMD functional score through single amino acid mutagenesis and deep mutation scanning (DMS) data including a mutant reads high expression and a mutant reads low expression.

The application discloses a protein secondary structure prediction method based on single-amino acid sequence characteristics, and belongs to the field of bioinformatics. The application solves the problem that the prediction effect of a model obtained by using an existing method is poor due to the limitation of evolutionary information feature acquisition and the fact that the model ignores the interdependent relationship between secondary structure labels in an amino acid sequence. In the feature processing stage, the application fuses feature representations generated by a protein language model and features obtained by using a hybrid coding mode of word embedding and position embedding. The fused features contain rich evolutionary information and fully acquire position information of amino acid residues in the sequence and the dependent relationship between the amino acid residues. The fused features are sequentially output through a Transformer encoder, a CNN and a BiLSTM to output global feature information, and the interaction between secondary structures of proteins in the sequence is considered by introducing a CRF, so that the protein secondary structure prediction accuracy is ensured. The method of the application can be applied to protein secondary structure prediction.

Described is a method for treating or preventing an infection, such as an infection associated with Staphylococcus epidermidis such as a Health-Care Associated infection, a wound, a sore, an ulcer, a burn. The method includes applying a treatment preparation to a treatment site, the treatment preparation has a first component having a single amino acid, said single amino acid being leucine, and a second component having an active chlorine compound.

The invention belongs to the technical field of carbon dioxide fixation. The invention provides a biological carbon sequestration enzyme mutant. The biological carbon sequestration enzyme mutant is obtained after threonine at the 398th site of RPE Rubisco is mutated; the amino acid sequence of the RPE Rubisco is as shown in SEQ ID NO. 1 in a sequence table. The threonine mutation is that threonine is mutated into alanine. According to the invention, through mutation of a single amino acid, the catalytic efficiency of Rubisco is successfully improved, and especially, the turnover number of enzyme is greatly increased and reaches 35.84 S <-1 >. Through reasonable structural design, the trade-off effect between kcat and km is successfully avoided, so that the Rubisco keeps high CO2 affinity while the activity is improved. The method is simple, is suitable for design and modification of other carboxylases, and has a wide application prospect.

The application provides a polypeptide and protein sequencing method, a kit and application. The polypeptide sequencing method comprises the following steps: S1, attaching a target polypeptide to a nanopore; S2, introducing a recognition molecule specifically recognizing a terminal amino acid of the target polypeptide into a nanopore sequencing system; under the action of an electric field, the detection of the terminal amino acid of the target polypeptide is realized by utilizing the electric signal change generated in the binding and dissociation process of the recognition molecule and the target polypeptide. By utilizing the unique electric signal change generated in the binding and dissociation process of the recognition molecule and the terminal amino acid of the target polypeptide, the detection of a single amino acid is realized.

The invention provides a synthetic method of bionic collagen hexapeptide, and belongs to the technical field of biological medicine. According to the method, single amino acid is used as a raw material, condensation, selective deprotection and fragment condensation are sequentially carried out through an activated ester method, and finally the bionic collagen hexapeptide shown in the formula (I) is prepared. According to the method, an expensive solid-phase synthesis carrier is abandoned, the raw material cost is low, a liquid-phase synthesis route is simple and convenient to operate, large-scale amplification is easy, side reactions are effectively controlled through a reasonable protection strategy, the product purity is high, and an efficient and economical preparation way is provided for development of related biological materials. Formula (I).

A lipid-vitamin conjugate based on an amino acid structure and a synthesis method therefor. The head of the lipid-vitamin conjugate consists of a single amino acid or a plurality of amino acids, and the tail thereof consists of a hydrophobic vitamin and a fatty chain. The molecular weight of the amino acid-based lipid-vitamin conjugate is 1000-2000 Da. The synthesis method is a solid-phase synthesis method based on polypeptide synthesis, and the method comprises: first attaching the carbon terminus of an amino acid to a 2-chlorotrityl chloride resin, then carrying out condensation-washing-deprotection-washing cycle synthesis, and finally, coupling a vitamin and a fatty chain to the nitrogen terminus to obtain a lipid-vitamin conjugate based on an amino acid structure. The synthesized lipid-vitamin conjugate is cleaved from the resin by means of a cleavage solution, and soluble by-products are removed from the organic solvent by means of a non-polar solvent. The method features ease of operation, high product purity, minimal complex factors requiring control, and high reproducibility.

This invention discloses a nanopore peptide sequencing method based on host-guest interactions. The method includes the following steps: 1) sequentially hydrolyzing the peptide to be sequenced into monomeric amino acids using a proteolytic enzyme; 2) linking the monomeric amino acids to a load molecule containing the guest molecule structure; 3) performing single-channel recording in a nanopore after the host molecule and guest molecule have bound. This invention provides a de novo sequencing method that utilizes the sequential hydrolysis of peptides by exopeptidases to obtain the peptide sequence information; by linking the load molecule to a single amino acid, the current blocking ratio of the characteristic signals generated after the binding of 20 encoding amino acids to the load molecule is accurately measured. The characteristic current is contributed by a single amino acid, eliminating decoding difficulties; the target peptide dosage is small, and the detection limit is low; the experimental operation is safe and simple, with most reactions carried out in an aqueous phase, without involving toxic reagents, making it green and safe.

SIRP1alpha-41BBL fusion proteins are provided. Accordingly, there is provided a SIRPalpha-41BBL fusion protein comprising a single amino acid linker between the SIRPalpha and the 41BBL. Also there is provided a SIRPalpha-41BBL fusion protein in a form of at least a homo-trimer. Also provided are polynucleotides and nucleic acid constructs encoding the SIRP1alpha-41BBL fusion protein, host-cells expressing the SIRP1alpha-41BBL fusion protein and methods of use thereof.

The invention relates to a GA sensitive type male sterility gene and application thereof in corn hybrid seed production, and belongs to the technical field of plant gene breeding, the open reading frame sequence of the gene is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, the mutation of the gene enables male flower filaments of a receptor plant not to stretch out and not to bloom and loosen, and the gene can be applied to corn hybrid seed production. Further, a receptor plant shows male sterility. The invention finds and verifies that the mutation of the GA sensitive male sterility gene can cause the maize to show the male sterility character, and the mutant material with single amino acid replacement has no significant change in vegetative growth and fruiting quantity, but still stably shows the phenotype of GA sensitive male sterility, and the obtained mutant material can be used for hybrid seed production and can be applied to the field of hybrid seed production. The fertility of the mutant material can be recovered by spraying gibberellin in one week of heading, and the mutant material can be used for selfing seed reproduction, so that a new direction and a theoretical basis are provided for corn hybrid seed production.

This invention belongs to the fields of molecular genetics and genetic engineering, and discloses a peanut plant type regulating gene, AhERECT1, its mutants, and their applications. The nucleotide sequence of the peanut plant type regulating gene AhERECT1 is shown in SEQ ID NO.1. Its mutant is caused by a single amino acid deletion due to the deletion of nucleotides 38-40 (CTC) in the open reading frame of the gene shown in SEQ ID NO.1, and its nucleotide sequence is shown in SEQ ID NO.2. This invention obtained the AhERECT1 gene through linkage mapping, fine mapping, and transcriptome analysis. Using CRISPR / Cas9 gene editing technology, the erect peanut variety Yuhua 9326 was successfully modified into a semi-vine type through targeted editing. The gene and its mutants of this invention provide key gene resources and efficient technical support for the precise improvement of peanut plant type, and can be widely applied to the breeding of peanut varieties with different plant types, which is of great significance for improving peanut yield and quality.

This invention belongs to the field of biotechnology, specifically relating to a hybridoma cell line secreting monoclonal antibodies against Clostridium perfringens ETX and its applications. Based on natural toxins, this invention establishes an indirect ELISA detection method using a non-toxic ETX single-amino acid site protein mutant. A series of hybridoma cell lines secreting monoclonal antibodies against Clostridium perfringens ETX were screened. These hybridoma cell lines can stably and efficiently secrete both neutralizing monoclonal antibodies and non-neutralizing monoclonal antibodies, and can be mass-produced on a large scale. This invention is the first to establish an antigen ELISA detection method for Clostridium perfringens ETX using monoclonal antibodies. This method features simple sample handling, low cost, rapid reaction, and high specificity. It can also provide a reference for the diagnosis of Clostridium perfringens infection and lay the foundation for research on alternative methods for testing the efficacy of related vaccines.

The invention relates to the technical field of polypeptide drugs, and provides a synthesis method of abparatide, which comprises the following steps: by taking Rink Amide AM resin, Rink MBHA resin or Rink Amide resin as a solid-phase carrier, sequentially coupling single amino acid and 19-21 tripeptide fragments or 19-22 tetrapeptide fragments from N terminal to C terminal through a solid-phase synthesis method according to a primary sequence of abparatide, so as to obtain abparatide peptide resin; the abparatide peptide resin is cracked to obtain abparatide, and a coupling agent / alkali adopted for coupling is selected from at least one of Oxyma / DIC, COMU / DIEA, COMU / TMP, PyOxim / DIEA and TPTU / DIEA. The abparatide crude peptide synthesized by the method disclosed by the invention is high in yield and high in purity, and the synthesis method disclosed by the invention shortens the production time, reduces the production cost and is beneficial to commercial production.

The invention discloses a rice essential gene ELD1 and application of the rice essential gene ELD1 in the aspect of adjusting the heading period. A new gene ELD1 for regulating and controlling the heading stage of rice is cloned through a forward genetics means. The ELD1 has the effect of inhibiting rice heading under the long-day condition, and early heading is caused by amino acid mutation. Although ELD1 knockout homozygosis causes embryo lethal, the yield of a plot is only reduced by 5.9% and the daily average yield is not obviously changed under the condition that the eld1 mutant is 10 days early in heading. According to the invention, directed evolution of the ELD1 is successfully realized by using a CRISPR-based cytosine base editor, a new heading stage material is created, and it is indicated that application of the ELD1 to rice heading stage breeding is feasible. Therefore, the invention provides a valuable target spot for regulating and controlling the heading period of the rice so as to improve the regional adaptability.

The invention relates to a culture medium suitable for cell culture and a preparation method thereof. The media can be prepared essentially by dissolving or mixing individual media components in water or in a suitable aqueous buffer. The important step of culture medium preparation is a sterilization step. The medium according to the invention may comprise a protein hydrolysate as an amino acid source. The invention also provides advantageous methods of hydrolyzing proteins into shorter peptide chains and / or single amino acids.

The present application relates to a GA sensitive male sterile gene and its application in corn hybrid seed production, and belongs to the technical field of plant gene breeding. The open reading frame sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the protein encoded by the gene is shown as SEQ ID NO. 2. The mutation of the gene makes the anther filaments of the male flowers of the recipient plants unable to stretch out and shed pollen, and further makes the recipient plants exhibit male sterility. The present application finds and verifies that the mutation of the GA sensitive male sterile gene can cause the corn to exhibit male sterility, and the single amino acid substitution mutant material has no significant change in the vegetative growth and seed setting amount, but still stably exhibits the phenotype of GA sensitive male sterility. The obtained mutant material can be used for hybrid seed production, and spraying gibberellin when the corn is in the stage of heading for one week can restore the fertility of the mutant material and be used for self-crossing breeding, thereby providing a new direction and theoretical basis for corn hybrid seed production.

The invention discloses a thymus dependent lymphocyte antigen epitope peptide of a lung cancer associated antigen Cyfra21-1 and application, and belongs to the field of medical immunology and oncology. The invention relates to a thymus dependent lymphocyte antigen epitope peptide of a lung cancer associated antigen Cyfra21-1. The amino acid sequence of the epitope peptide comprises any one or more of the following: EVKIRDWYQK; a YQKQGPGPSR (YQKQGPGPSR) A DYSHYYTTI (Transmission Time Interval); the amino acid sequence is an amino acid sequence obtained by removing or replacing a single amino acid of the amino acid sequence. The epitope peptide disclosed by the invention can be specifically combined with cytotoxic thymus dependent lymphocytes to stimulate the cytotoxic thymus dependent lymphocytes to activate, proliferate and differentiate, so that an anti-tumor immune effect is exerted; the antigen peptides can be used for preparing therapeutic and preventive vaccines for lung cancer, can also be used for preparing quantitative detection reagents for detecting specific T cells of the lung cancer related antigen Cyfra21-1, and have potential application value in prevention, treatment and diagnosis of lung cancer.

The invention discloses a bactericide synergist, a corrosion biofilm sterilization composition and a use method, and relates to the technical field of oil and gas field microbial corrosion protection, the bactericide synergist comprises D tyrosine with the concentration of 0.1-2 ppm, and a single amino acid has a good synergistic effect on a bactericide, and the invention also provides the corrosion biofilm sterilization composition. Comprising dibromo cyanoacetamide, nano-silver, limonene and a bactericide synergist, has antibacterial and microbial corrosion resistant properties, and can be used in microbial corrosion protection operation of oil and gas fields.

The invention is applicable to the technical field of plant genetic engineering, and provides a soybean 1-aminocyclopropane-1-carboxylic acid synthase mutant based on single amino acid deletion, and the soybean 1-aminocyclopropane-1-carboxylic acid synthase mutant is named as # imgabs 0 # and # imgabs 1 #; the amino acid sequence of the # imgabs2 is as shown in SEQ ID NO. 1; the amino acid sequence of the # imgabs3 # is as shown in SEQ ID NO. 2. A functional site modification technology of the soybean 1-aminocyclopropane-1-carboxylic acid synthase is utilized to obtain the 1-aminocyclopropane-1-carboxylic acid synthase active mutant in which the activity of the 1-aminocyclopropane-1-carboxylic acid synthase is completely lost and the activity of C-S lyase is partially retained, and a novel target and a gene resource are provided for creation of soybean stress-resistant varieties. The soybean 1-aminocyclopropane-1-carboxylic acid synthase and the 1-aminocyclopropane-1-carboxylic acid synthase in other plants have high conservative property at the corresponding D site, and are important regulation and control sites of the double-enzyme activity of the 1-aminocyclopropane-1-carboxylic acid synthase, so that the same effect can be generated by performing similar deletion in homologous proteins of soybeans and other plants.

The invention discloses a tomato transcription factor SlTGA9 mutant and application thereof, lysine K at the 132 site of SlTGA9 is mutated into alanine A, and a mutant SlTGA9mut is obtained; the gene nucleotide sequence of the mutant SlTGA9mut is as shown in SEQ ID NO: 3, and the amino acid sequence of the mutant SlTGA9mut is as shown in SEQ ID NO: 4; according to the invention, only a single amino acid site is changed, so that multiple-effect side effects caused by traditional gene editing are avoided. The overexpression of the SlTGA9mut can significantly accelerate the ripening process of the fruits, and does not affect the growth and development of the plants. The mutant protein is stably expressed in a cell nucleus, so that the continuity of a regulation function is ensured. The inhibition effect of SlTGA9 on fruit ripening is relieved through single-point mutation, and an efficient and safe tomato breeding technology is provided. The mutant can be used for cultivating an early-maturing and high-quality new tomato variety, and has remarkable economic value and application prospect.

The invention provides a synthetic method of cyclohexapeptide-9, and belongs to the technical field of skin care product raw materials. According to the synthesis method, single amino acid is taken as a starting raw material, and cyclic hexapeptide-9 shown in the formula (I) is efficiently prepared through step-by-step condensation by an activated ester method, orthogonal selective deprotection and coupling reaction. The method overcomes the problems of a solid-phase synthesis method, avoids the use of expensive dipeptide raw materials in an existing liquid-phase synthesis method, and has the advantages of low raw material cost, simple synthesis route, controllable side reaction, high product purity and easiness in process amplification. Formula (I)

Provided are vitamin AD soft capsules and a method for producing the same, which have improved stability and bioavailability in soft capsule formulations. [Solution] We have discovered that adding CGKRK peptide as an anti-aging agent to a gelatin-based wall material has a superior anti-gelatin aging effect than a single amino acid, and that even with a lower added amount, it can significantly alleviate the problems of soft capsule collapse, delayed dissolution, and reduced dissolution rate, and further improve the stability and bioavailability of vitamin AD soft capsule formulations.

The invention provides an intermittent urinary catheter comprising a surface comprising at least one amphipathic antimicrobial peptide, wherein only a single amino acid of the at least one amphipathic antimicrobial peptide is tryptophan or a derivative thereof, and at least one amino acid is arginine or a derivative thereof.