5 results about "Single amino acid" patented technology

The application provides a polypeptide and protein sequencing method, a kit and application. The polypeptide sequencing method comprises the following steps: S1, attaching a target polypeptide to a nanopore; S2, introducing a recognition molecule specifically recognizing a terminal amino acid of the target polypeptide into a nanopore sequencing system; under the action of an electric field, the detection of the terminal amino acid of the target polypeptide is realized by utilizing the electric signal change generated in the binding and dissociation process of the recognition molecule and the target polypeptide. By utilizing the unique electric signal change generated in the binding and dissociation process of the recognition molecule and the terminal amino acid of the target polypeptide, the detection of a single amino acid is realized.

This invention belongs to the fields of molecular genetics and genetic engineering, and discloses a peanut plant type regulating gene, AhERECT1, its mutants, and their applications. The nucleotide sequence of the peanut plant type regulating gene AhERECT1 is shown in SEQ ID NO.1. Its mutant is caused by a single amino acid deletion due to the deletion of nucleotides 38-40 (CTC) in the open reading frame of the gene shown in SEQ ID NO.1, and its nucleotide sequence is shown in SEQ ID NO.2. This invention obtained the AhERECT1 gene through linkage mapping, fine mapping, and transcriptome analysis. Using CRISPR / Cas9 gene editing technology, the erect peanut variety Yuhua 9326 was successfully modified into a semi-vine type through targeted editing. The gene and its mutants of this invention provide key gene resources and efficient technical support for the precise improvement of peanut plant type, and can be widely applied to the breeding of peanut varieties with different plant types, which is of great significance for improving peanut yield and quality.

This invention relates to a culture medium suitable for cell culture and a process for preparing the same. The culture medium can essentially be prepared by dissolving or mixing individual medium components in water or a suitable aqueous buffer. A key step in the preparation of the medium is a sterilization step. The culture medium according to the present invention may contain protein hydrolysates as an amino acid source. A favorable process for protein hydrolysis to shorter peptide chains and / or single amino acids is also provided by this invention.

The application relates to the technical field of post-translational modification site prediction, and discloses a multi-modal cross-view protein post-translational modification site prediction method and system, which comprises the following steps: acquiring protein peptide segments and constructing sequence modality-based feature representation, decomposing the protein into single amino acid units and capturing local structure information of each residue, constructing structure modality-based feature representation according to the local structure information, combining the sequence modality-based feature representation and the structure modality-based feature representation to construct initial cycle state features, using RIDCGA to perform cycle iteration fusion on the initial cycle state features to obtain fusion features, and performing protein post-translational modification site prediction according to the fusion features. The application can improve the prediction accuracy in a complex real biological data scene.

This invention belongs to the field of preparation and stabilization technology of functional inorganic nanocomposites, specifically disclosing an ACC nanoparticle stabilizer and its application. This invention uses a single amino acid such as aspartic acid, glutamic acid, and lysine, or a mixture of multiple amino acids, as the amino acid source, and phosphoric acid solution as the phosphorus source. By controlling the reaction temperature at 130-220℃ and the reaction time at 1-5 hours, hydrothermal polymerization is achieved to prepare ACC nanoparticle stabilizers. The hydrothermal method does not require complex catalysts, has mild reaction conditions, and is suitable for acidic, alkaline, and mixed amino acid systems, exhibiting strong versatility. The hydrothermal product can be directly concentrated and used without separation and purification, which not only reduces preparation costs but also helps improve the stability of ACC nanoparticles, solving the problem of insufficient long-term stability and easy crystallization of ACC nanoparticles. It has the advantages of being green and efficient, highly controllable, and biosafe.