34 results about "RuBisCO" patented technology

Provided is a polypeptide combination, which comprises a Rubisco polypeptide from a species of the family Chlamydomonadaceae, and a fusion polypeptide comprising an EPYC1 polypeptide of a species of the family Chlamydomonadaceae and a mineralizing peptide, wherein the mineralizing peptide can convert a metal-containing compound into a compound capable of converting light energy into chemical energy, such as a metal oxide. Further provided are a polynucleotide or a vector encoding the polypeptide combination, a microorganism containing the polypeptide combination, the polynucleotide or the vector, and a method of using the microorganism.

Described herein are genelight cultures, extracts and related methods. In one aspect, the method of making a genelight culture or extract includes the steps of (a) making a DNA construct containing genes for producing a heat shock protein, RuBisCO large subunit 1, tonB, hydrogenase, and a P-type ATPase, (b) introducing the DNA construct into host microbial cells via transformation or transfection, and (c) culturing the microbial cells to produce the genelight cultures and extracts. The compositions of these cultures and extracts can be tailored to have specific properties such as the ability to provide power to a light emitting diode. The cultures and extracts have further uses including enhancing the growth of plants and as supplemental nutrients of cultures of industrially important microorganisms. The cultures and extracts further have UV-protective properties. Also described herein are microbial electric circuits containing the cultures and extracts described herein.

The present invention comprises the deployment of a product known as RubisCO Climate Vaccine® or simply RCV which contains a specialised adapted culture of psychrophile algae and accelerators. This RCV is inoculated into targeted tropospheric clouds. The cumulatively large air-liquid optical interface area of clouds enables conditions for potential exponential photosynthetic algal growth and replication during which carbon dioxide is processed and oxygen released directly in the troposphere. Optimal inoculation sites are determined by altitude, humidity, temperature, weather patterns and predicted direction and duration of travel for precipitation, The biomass produced in this process is intended to be precipitated over mid-ocean where it is sequestered within natural ocean food chains or sedimented as detritus essentially permanently. This nature-based solution through repeated deployments offers a feasible scalable pathway toward potential climate change reversal within a decade with minimal or no adverse environmental impact.

The invention discloses an enrichment method suitable for trace leaf plasma membrane protein, and belongs to the technical field of biology. According to the method, a mode of combining column type separation with density gradient centrifugation is adopted, components highly enriched in plasma membrane protein can be obtained through the processes of adjusting the material dosage, the centrifugation mode and the like, and the enrichment purity is improved while the separation time is saved. Compared with an untreated component, the plasma membrane labeled protein H + ATPase in the enriched component is obviously increased; meanwhile, compared with components only subjected to column type separation, chloroplast labeled protein RuBisCO ACT, cell nucleus labeled protein Histone H3 and cytoplasm labeled protein UGP are remarkably reduced. According to the method, an effective system suitable for extracting and purifying the plasma membrane protein of the catharanthus roseus leaves is established for the first time, and the method has reference significance for constructing plasma membrane protein enrichment of other plant leaves.

This invention discloses a diagnostic and predictive method and system for rice post-drought rehydration recovery capacity based on photosynthetic and molecular indicators, belonging to the field of rice drought resistance diagnostic technology. This invention collects leaf samples from rice at the normal water supply period, the end of drought, and the rehydration recovery period, respectively, to obtain net photosynthetic rate, Rubisco enzyme activity, and expression levels of several characteristic genes, constructing normalized photosynthetic recovery indicators, biochemical recovery indicators, and molecular recovery indicators; and constructs a comprehensive diagnostic index by weighted coupling of these indicators. This method effectively reduces the fluctuation bias caused by drought environmental disturbances to single indicators; while being compatible with special physiological scenarios such as abnormally elevated enzyme activity under drought stress, it improves the robustness of diagnostic results by averaging the expression levels of multiple genes, achieving quantitative diagnosis and accurate prediction of rice rehydration compensation capacity; it solves the problem of existing technologies using single indicators and difficulty in quantifying the physiological homeostasis reconstruction process.

The invention discloses a method for producing astaxanthin in cotton BKT Gene and application, belonging to the field of plant genetic engineering technology. In cotton, the enhanced constitutive 2×35S promoter is used to drive the tobacco Rubisco signal peptide to induce BKT The gene is expressed in cotton chloroplasts, successfully establishing an astaxanthin biosynthetic pathway, enabling astaxanthin production in various cotton tissues, including leaves, stems, anthers, sepals, and seeds. Testing of transgenic cotton seeds revealed a significant increase in weight and size, but a decrease in gossypol content. Therefore, the method of the present invention can create transgenic cotton plants rich in astaxanthin, with larger and heavier cotton seeds, lower gossypol content, and a more nutritious and less toxic profile. This method can be used to create cotton plants that are bio-factories for astaxanthin production, providing a stable, low-cost, and effective alternative to traditional astaxanthin sources.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and / or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and / or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg / g; and a heavy metal content of less than about 60 μg / g.

Compositions and methods of the invention relate to modified yeast cells that heterologously express a RuBisCo enzyme from specific iron oxidizing bacteria species. The modified yeast cells exhibit reduced glycerol and acetic acid accumulation in fermentation while maintaining high ethanol production, making them useful for large scale ethanol production from starch substrates, where glycerol and acetic acid represent undesired by-products.

The invention discloses an application of a soybean RuBisCO activating enzyme family gene GmRCA11 in improvement of soybean quality. The nucleotide sequence of the soybean GmRCA11 protein coding gene GmRCA11 is shown as SEQ ID NO. 1, and the nucleotide sequence of the soybean GmRCA11 protein coding gene Genetic transformation is carried out on the PBA002-GmRCA11 overexpression vector in soybeans, and it is found that compared with a receptor Jack, the protein content in grains of an overexpression material is remarkably increased. Therefore, the soybean GmRCA11 protein coding gene GmRCA11 disclosed by the invention can be applied to the aspect of improving the soybean protein through genetic engineering.

The application provides a use of an AP1 gene, and relates to the technical field of agricultural molecular breeding. The AP1 gene or a coded protein thereof is used for regulating the agronomic traits of plants or preparing an inhibitor or a composition for regulating the agronomic traits of plants; the regulation of the agronomic traits of plants specifically comprises: increasing the photosynthetic rate of plant leaves under salt stress, increasing the chlorophyll content, promoting the germination rate and / or total leaf area per plant, and the like. The AP1 gene can be used as a target for regulating the traits of plants and applied to plant breeding. The AP1 gene can effectively regulate the fluctuating light use efficiency of plants, increase the photosynthetic efficiency and Rubisco protein content under high-salt stress, and increase the resistance to adversity such as high-salt conditions.

Provided are methods of producing an isolate of soluble proteins from moringa leaves, and methods of preparing a powder of soluble proteins from moringa leaves. Also provided are compositions-of-matter comprising a chlorophyll-free preparation of soluble proteins of moringa leaves, wherein the soluble proteins comprise large and small Rubisco protein subunits, a preparation comprising the composition-of-matter, and edible, cosmetic and pharmaceutical compositions comprising same.

The invention discloses an application of a coding gene GmRCA03 of a soybean Rubisco activating enzyme. The invention further discloses application of the soybean Rubisco activating enzyme encoding gene GmRCA03 as shown in SEQ ID NO.1 in genetic engineering modification of the photosynthetic rate and the seed yield of soybeans. Through overexpression of the GmRCA03 gene, the photosynthetic rate and the seed yield of soybeans can be improved. Mutation of the gene reduces the photosynthetic rate and seed yield of soybean. The overexpressed GmRCA03 gene reduces the lodging rate and improves the photosynthetic rate seed yield in a soybean and corn strip-shaped composite planting mode. The soybean Rubisco activating enzyme coding gene GmRCA03 disclosed by the invention can be transformed into soybeans through genetic engineering, and finally, the photosynthetic rate and the seed yield of the soybeans are positively regulated and controlled.

To provide a method for maintaining or increasing the content of rubisco protein in algal cells.SOLUTION: A method for producing a protein, comprising culturing an alga in a medium containing sulfate ions and / or thiosulfate ions under conditions that do not deplete the sulfate ions and / or thiosulfate ions, thereby maintaining or increasing the content of a rubisco protein in the algal body of the alga.SELECTED DRAWING: Figure 1

The application discloses application of a corn ZmRbcL_n1 gene in improvement of photosynthesis efficiency of plants and relates to the technical field of plant genetic engineering. Specifically, the protein coded by the corn ZmRbcL_n1 gene is different from the traditional RbcL protein structure, the protein structure has a molecular chaperone domain for promoting complex assembly, and fluorescence quantification shows that the corn ZmRbcL_n1 gene is highly expressed in leaves, which indicates that the protein may promote photosynthetic carbon fixation of leaves by mechanisms such as promoting RuBisco complex assembly. Further, the application constructs an expression vector for over-expressing the corn ZmRbcL_n1 gene, and the gene is introduced into Arabidopsis thaliana plants by an agrobacterium-mediated method, and it is found that over-expression of the gene can promote biomass accumulation and seed yield of the Arabidopsis thaliana, so that it is indicated that the corn ZmRbcL_n1 gene has great application prospect in improving photosynthesis and increasing carbon fixation efficiency of plants.

The invention discloses a Bayesian inference-based high-temperature stress vegetation stomata adjustment modeling system and method, and relates to the technical field of ecological process simulation. A data acquisition module is used for acquiring and preprocessing meteorological driving data; the stomatal conductance calculation module is used for calculating basic stomatal conductance based on a Jarvis stomatal conductance empirical model; the high-temperature stress correction module is used for correcting Rubisco enzyme related parameters based on a temperature power function and constructing a high-temperature stress stomatal conductance correction function through a Logistic function; the correction parameter inversion module is used for inverting optimal parameters in combination with the actually measured carbon water flux data, the Bayesian theorem and the Monte Carlo-Markov chain algorithm; and the carbon-water flux simulation module is used for substituting the optimal parameters into a high-temperature stress stomatal conductance correction function to perform high-temperature stress correction on the basic stomatal conductance, and after the corrected stomatal conductance is obtained, the corrected stomatal conductance is coupled to the ecological process model to simulate the carbon-water flux process. The simulation precision of the model at extreme high temperature can be improved.

This invention provides a method for designing scaffold proteins that mediate RuBisCO aggregation in crop plants. Using a known scaffold protein as a template, this method generates sequences through the following steps: a. RFdiffusion scaffold protein backbone generation; b. MPNN sequence generation; c. AlphaFold structure prediction; and d. Optimizing the optimal sequence by evaluating the generated scaffold protein sequences using one or more scoring metrics. Unlike previous research focused on RuBisCO itself, this invention shifts the focus to the design and optimization of scaffold proteins. By optimizing the parameter evaluation method for protein design, novel scaffold proteins were designed, addressing the bottleneck of traditional methods in forming the RuBisCO concentration module and opening up a new research direction.

Provided is a polypeptide combination, which comprises a Rubisco polypeptide from a species of the family Chlamydomonadaceae, and a fusion polypeptide comprising an EPYC1 polypeptide of a species of the family Chlamydomonadaceae and a mineralizing peptide, wherein the mineralizing peptide can convert a metal-containing compound into a compound capable of converting light energy into chemical energy, such as a metal oxide. Further provided are a polynucleotide or a vector encoding the polypeptide combination, a microorganism containing the polypeptide combination, the polynucleotide or the vector, and a method of using the microorganism.

The present invention provides a combination of polypeptides comprising a Rubisco polypeptide from a species of the family Chlamydomonas, and an EPYC1 polypeptide from a species of the family Chlamydomonas. The invention also provides polynucleosides or vectors encoding the polypeptide combinations, combinations comprising the polypeptides, microorganisms comprising the polynucleosides or vectors, and methods of using the microorganisms.

The present invention relates to microgel compositions comprising about 1.0 wt% to about 15 wt% RuBisCO protein and water or a plant-based milk. Further, microgel emulsion compositions are described. The microgel compositions and microgel emulsion compositions according to the present invention can be used in the preparation of dairy analogues, in particular acidified dairy analogues such as yogurt. The present invention further relates to the method of preparation of such compositions.

The purpose of the present invention is to provide a method for maintaining or increasing the content of rubisco protein in an alga body. Provided is a method for producing a protein, which makes it possible to maintain or increase the content of rubisco protein in an alga body of an alga by adding sulfate ions and / or thiosulfate ions to a culture medium and culturing the alga under conditions where the sulfate ions and / or the thiosulfate ions are not depleted.

The application discloses a high-temperature stress vegetation stomatal regulation modeling system and method based on Bayesian inference, and relates to the technical field of ecological process simulation, wherein: a data acquisition module is used for collecting and preprocessing meteorological driving data; a stomatal conductance calculation module is used for calculating basic stomatal conductance based on a Jarvis stomatal conductance empirical model; a high-temperature stress correction module is used for correcting Rubisco enzyme related parameters based on a temperature power function, and constructing a high-temperature stress stomatal conductance correction function through a Logistic function; a correction parameter inversion module is used for inversing optimal parameters in combination with measured carbon flux data, Bayesian theorem and a Monte Carlo-Markov chain algorithm; and a carbon flux simulation module is used for substituting the optimal parameters into the high-temperature stress stomatal conductance correction function to correct the basic stomatal conductance under high-temperature stress, obtaining corrected stomatal conductance, and then coupling to an ecological process model to simulate a carbon flux process. The application can improve the simulation accuracy of the model under extreme high temperature.

The invention relates to application of 2-hydroxyphosphono-1, 4-naphthalenedione in a photosynthetic agent for plant leaves and the plant photosynthetic agent, and the plant photosynthetic agent comprises the following raw materials in percentage by mass: 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4- The cleaning agent is prepared from 0.5%-2% of 1, 4-naphthalenedione, 5%-10% of a surfactant, 3%-8% of a penetrating agent, 0.1%-0.5% of a stabilizer, 0.2%-1% of a thickening agent, 1%-3% of a nutrition auxiliary agent, 1%-2% of a buffering agent and the balance of a cosolvent. According to the invention, HPN is combined with Rubisco active sites to preferentially block the combination of O2 and promote the combination of CO2 at the same time, so that light breathing is inhibited; the phosphoryl and naphthalenedione structures can change the local environment of active sites (such as reducing the solubility of O2 or increasing the concentration of CO2) and enhance the activity of carboxylase; hPN is combined with metal ions (such as Mg < 2 + >) of Rubisco, enzyme conformation change is induced, the affinity of CO2 is optimized, and the efficiency of photosynthesis is improved.

The present invention relates to microgel compositions comprising about 1.0 wt% to about 15 wt% RuBisCO protein and water or a plant-based milk. Further, microgel emulsion compositions are described. The microgel compositions and microgel emulsion compositions according to the present invention can be used in the preparation of dairy analogues, in particular acidified dairy analogues such as yogurt. The present invention further relates to the method of preparation of such compositions.

The invention discloses an engineering strain for efficiently producing L-methionine as well as a construction method and application of the engineering strain, and belongs to the technical field of gene engineering. The engineering strain overexpresses the following genes: a metA * gene, a yjaH gene, a metC gene, a cysEM201R gene, a serA * gene, a cysDN gene, a cysB gene, a pck gene, a zwf gene, a crp gene, a csgD gene, a prsD128A gene, a spoT gene, a RuBisCO gene, a groEL-groES gene and a metB * gene; expressing a prK gene; according to the engineering strain, the following genes are deleted on a genome: a metJ gene, a lacI gene, an rhtA gene, a metQIN gene, a pykF gene, a pykA gene, a purR gene, a sucCD gene and an aceA gene. The engineering strain disclosed by the invention can be used for efficiently producing L-methionine.

This invention discloses a method for rapidly isolating and purifying Rubisco from microalgae. The method includes the following steps: lysing the microalgae and filtering to obtain a supernatant of a crude protein extract; combining the supernatant of the crude protein extract with anion-exchange magnetic beads; separating the magnetic beads and eluting sequentially with a gradient elution buffer containing 40-550 mM NaCl to obtain Rubisco from the microalgae. Experiments have shown that the above-mentioned rapid method for isolating and purifying Rubisco can effectively isolate and purify Rubisco from the Chlamydomonas reinhardtii CC-125 strain. It is not only simple to operate, requiring only one step of directional enrichment, but also quick, with the entire process taking ≤3 hours. More importantly, it yields Rubisco with high purity and very few contaminating proteins. Therefore, the method provided by this invention can rapidly isolate and purify Rubisco and has significant application value.

Described herein are genelight cultures and extracts and methods of making and using thereof. In one aspect, the method of making a genelight culture or extract includes the steps of (a) making a DNA construct containing genes for producing a heat shock protein, RuBisCO large subunit 1, tonB, hydrogenase, and a P-type ATPase, (b) introducing the DNA construct into host microbial cells via transformation or transfection, and (c) culturing the microbial cells to produce the genelight cultures and extracts. The compositions of these cultures and extracts can be tailored to have specific properties such as the ability to provide power to a light emitting diode. The cultures and extracts have further uses including enhancing the growth of plants and as supplemental nutrients of cultures of industrially important microorganisms. The cultures and extracts further have UV-protective properties. Also described herein are microbial electric circuits containing the cultures and extracts described herein.

The invention discloses a carbon fiber fixing vibrio and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Cellvibrio sp. Is separated, the strain is preserved in the China General Microbiological Culture Collection Center on May 9, 2025, and the preservation number is CGMCC No.34469. The invention also discloses a method for preparing the Cellvibrio sp. The Cellvibrio sp GT-5 shows a remarkable carbon sequestration capability in a culture medium without a carbon source. The activity of the RubisCO enzyme reaches 78.8 U / mL, and the RubisCO enzyme can catalyze the combination of C5 and CO2 and can be applied to the field of microbial carbon sequestration, so that the biological function cognition of Cellvibrio is widened, and a new method is provided for immobilizing CO2.

High protein content flowable Rubisco gels and process for preparation thereof are described. The gels are useful for the formulation of high protein consumer products, such as sports gels.

The invention discloses an application of a soybean RuBisCO activating enzyme family gene GmRCA11 in improvement of soybean quality. The nucleotide sequence of the soybean GmRCA11 protein coding gene GmRCA11 is shown as SEQ ID NO. 1, and the nucleotide sequence of the soybean GmRCA11 protein coding gene Genetic transformation is carried out on the PBA002-GmRCA11 overexpression vector in soybeans, and it is found that compared with a receptor Jack, the protein content in grains of an overexpression material is remarkably increased. Therefore, the soybean GmRCA11 protein coding gene GmRCA11 disclosed by the invention can be applied to the aspect of improving the soybean protein through genetic engineering.

The application discloses an engineering bacterium for synthesizing PHA by using carbon dioxide and a construction method and application thereof. First, the Rubisco gene from Methanosaeta sp. GP6 is integrated on the genome, and the original promoter is replaced; then, the hydrogenase gene from Citrobacter freundii ATCC 29220 is integrated on the genome, the lactate dehydrogenase gene in the competitive pathway is knocked out, and the PHA synthesis gene is overexpressed, so as to further improve the PHA yield. The engineering bacterium provided by the application adopts a high-density fermentation culture method, the PHA yield in a 5L fermenter can reach 83.5% of the cell dry weight, and the cell dry weight reaches 22.14g / L. The application has important application significance for industrialized production of PHA.