14 results about "RuBisCO" patented technology

The invention discloses an enrichment method suitable for trace leaf plasma membrane protein, and belongs to the technical field of biology. According to the method, a mode of combining column type separation with density gradient centrifugation is adopted, components highly enriched in plasma membrane protein can be obtained through the processes of adjusting the material dosage, the centrifugation mode and the like, and the enrichment purity is improved while the separation time is saved. Compared with an untreated component, the plasma membrane labeled protein H + ATPase in the enriched component is obviously increased; meanwhile, compared with components only subjected to column type separation, chloroplast labeled protein RuBisCO ACT, cell nucleus labeled protein Histone H3 and cytoplasm labeled protein UGP are remarkably reduced. According to the method, an effective system suitable for extracting and purifying the plasma membrane protein of the catharanthus roseus leaves is established for the first time, and the method has reference significance for constructing plasma membrane protein enrichment of other plant leaves.

This invention discloses a diagnostic and predictive method and system for rice post-drought rehydration recovery capacity based on photosynthetic and molecular indicators, belonging to the field of rice drought resistance diagnostic technology. This invention collects leaf samples from rice at the normal water supply period, the end of drought, and the rehydration recovery period, respectively, to obtain net photosynthetic rate, Rubisco enzyme activity, and expression levels of several characteristic genes, constructing normalized photosynthetic recovery indicators, biochemical recovery indicators, and molecular recovery indicators; and constructs a comprehensive diagnostic index by weighted coupling of these indicators. This method effectively reduces the fluctuation bias caused by drought environmental disturbances to single indicators; while being compatible with special physiological scenarios such as abnormally elevated enzyme activity under drought stress, it improves the robustness of diagnostic results by averaging the expression levels of multiple genes, achieving quantitative diagnosis and accurate prediction of rice rehydration compensation capacity; it solves the problem of existing technologies using single indicators and difficulty in quantifying the physiological homeostasis reconstruction process.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and / or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and / or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg / g; and a heavy metal content of less than about 60 μg / g.

The application provides a use of an AP1 gene, and relates to the technical field of agricultural molecular breeding. The AP1 gene or a coded protein thereof is used for regulating the agronomic traits of plants or preparing an inhibitor or a composition for regulating the agronomic traits of plants; the regulation of the agronomic traits of plants specifically comprises: increasing the photosynthetic rate of plant leaves under salt stress, increasing the chlorophyll content, promoting the germination rate and / or total leaf area per plant, and the like. The AP1 gene can be used as a target for regulating the traits of plants and applied to plant breeding. The AP1 gene can effectively regulate the fluctuating light use efficiency of plants, increase the photosynthetic efficiency and Rubisco protein content under high-salt stress, and increase the resistance to adversity such as high-salt conditions.

Provided are methods of producing an isolate of soluble proteins from moringa leaves, and methods of preparing a powder of soluble proteins from moringa leaves. Also provided are compositions-of-matter comprising a chlorophyll-free preparation of soluble proteins of moringa leaves, wherein the soluble proteins comprise large and small Rubisco protein subunits, a preparation comprising the composition-of-matter, and edible, cosmetic and pharmaceutical compositions comprising same.

To provide a method for maintaining or increasing the content of rubisco protein in algal cells.SOLUTION: A method for producing a protein, comprising culturing an alga in a medium containing sulfate ions and / or thiosulfate ions under conditions that do not deplete the sulfate ions and / or thiosulfate ions, thereby maintaining or increasing the content of a rubisco protein in the algal body of the alga.SELECTED DRAWING: Figure 1

The application discloses application of a corn ZmRbcL_n1 gene in improvement of photosynthesis efficiency of plants and relates to the technical field of plant genetic engineering. Specifically, the protein coded by the corn ZmRbcL_n1 gene is different from the traditional RbcL protein structure, the protein structure has a molecular chaperone domain for promoting complex assembly, and fluorescence quantification shows that the corn ZmRbcL_n1 gene is highly expressed in leaves, which indicates that the protein may promote photosynthetic carbon fixation of leaves by mechanisms such as promoting RuBisco complex assembly. Further, the application constructs an expression vector for over-expressing the corn ZmRbcL_n1 gene, and the gene is introduced into Arabidopsis thaliana plants by an agrobacterium-mediated method, and it is found that over-expression of the gene can promote biomass accumulation and seed yield of the Arabidopsis thaliana, so that it is indicated that the corn ZmRbcL_n1 gene has great application prospect in improving photosynthesis and increasing carbon fixation efficiency of plants.

The present invention relates to microgel compositions comprising about 1.0 wt% to about 15 wt% RuBisCO protein and water or a plant-based milk. Further, microgel emulsion compositions are described. The microgel compositions and microgel emulsion compositions according to the present invention can be used in the preparation of dairy analogues, in particular acidified dairy analogues such as yogurt. The present invention further relates to the method of preparation of such compositions.

The application discloses a high-temperature stress vegetation stomatal regulation modeling system and method based on Bayesian inference, and relates to the technical field of ecological process simulation, wherein: a data acquisition module is used for collecting and preprocessing meteorological driving data; a stomatal conductance calculation module is used for calculating basic stomatal conductance based on a Jarvis stomatal conductance empirical model; a high-temperature stress correction module is used for correcting Rubisco enzyme related parameters based on a temperature power function, and constructing a high-temperature stress stomatal conductance correction function through a Logistic function; a correction parameter inversion module is used for inversing optimal parameters in combination with measured carbon flux data, Bayesian theorem and a Monte Carlo-Markov chain algorithm; and a carbon flux simulation module is used for substituting the optimal parameters into the high-temperature stress stomatal conductance correction function to correct the basic stomatal conductance under high-temperature stress, obtaining corrected stomatal conductance, and then coupling to an ecological process model to simulate a carbon flux process. The application can improve the simulation accuracy of the model under extreme high temperature.

The present invention relates to microgel compositions comprising about 1.0 wt% to about 15 wt% RuBisCO protein and water or a plant-based milk. Further, microgel emulsion compositions are described. The microgel compositions and microgel emulsion compositions according to the present invention can be used in the preparation of dairy analogues, in particular acidified dairy analogues such as yogurt. The present invention further relates to the method of preparation of such compositions.

This invention discloses a method for rapidly isolating and purifying Rubisco from microalgae. The method includes the following steps: lysing the microalgae and filtering to obtain a supernatant of a crude protein extract; combining the supernatant of the crude protein extract with anion-exchange magnetic beads; separating the magnetic beads and eluting sequentially with a gradient elution buffer containing 40-550 mM NaCl to obtain Rubisco from the microalgae. Experiments have shown that the above-mentioned rapid method for isolating and purifying Rubisco can effectively isolate and purify Rubisco from the Chlamydomonas reinhardtii CC-125 strain. It is not only simple to operate, requiring only one step of directional enrichment, but also quick, with the entire process taking ≤3 hours. More importantly, it yields Rubisco with high purity and very few contaminating proteins. Therefore, the method provided by this invention can rapidly isolate and purify Rubisco and has significant application value.

High protein content flowable Rubisco gels and process for preparation thereof are described. The gels are useful for the formulation of high protein consumer products, such as sports gels.

The present invention provides methods for the high-level production of recombinant human erythropoietin (rhuEPO) derivative, asialoerythropoietin (asialo-rhuEPO), in plants. The methods for producing asialo-rhuEPO comprise making a plant or at least one plant cell that comprises a promoter that drives expression in a plant cell operably linked to a polynucleotide encoding a human erythropoieting fusion protein and a promoter that drives expression in a plant cell operably linked to a polynucleotide encoding N-glycosylation modification enzyme, particularly a mammalian β1, 4-galactosyltransferase. The present invention further provides plants, plant cells, and seeds that have been genetically modified to produce high levels of asialo-rhuEPO. Additionally, provided are methods for purifying asialo-rhuEPO from plant tissues. Such methods comprise removing chlorophyll and / or RuBisCO protein from an aqueous extract of plant tissue comprising asialo-rhuEPO, binding the asialo-rhuEPO in the extract to an immune affinity column, and eluting the bound asialo-rhuEPO from immune affinity column.

The application discloses a carbon-fixing Vibrio fibrius and application thereof, and belongs to the technical field of microorganisms. Cellvibrio sp. The Vibrio fibrius is preserved in the China General Microbiological Culture Collection Center on May 9, 2025, and the preservation number is CGMCC No. 34469. The Vibrio fibrius GT-5 shows a significant carbon-fixing capacity in a carbon-free culture medium. Cellvibrio sp The RubisCO enzyme activity reaches 78.8 U / mL, the RubisCO enzyme can catalyze the combination of C5 and CO2, can be applied to the field of microbial carbon fixation, and the discovery widens the biological function cognition of the genus and provides a new method for fixing CO2. Cellvibrio ​