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44 results about "Gateway Technology" patented technology

The Gateway cloning System, invented and commercialized by Invitrogen since the late 1990s, is a molecular biology method that enables researchers to efficiently transfer DNA-fragments between plasmids using a proprietary set of recombination sequences, the "Gateway att" sites, and two proprietary enzyme mixes, called "LR Clonase", and "BP Clonase". Gateway Cloning Technique allows transfer of DNA fragments between different cloning vectors while maintaining the reading frame. Using Gateway, one can clone subclone DNA segments for functional analysis. The system requires the initial insertion of a DNA fragment into a plasmid with two flanking recombination sequences called “att L 1” and “att L 2”, to develop a “Gateway Entry clone” (special Invitrogen nomenclature).

Thing Internet gateway of heterogeneous wireless network and control method thereof

InactiveCN101951693ARealize ad hoc networkOvercoming Accessibility LimitationsNetwork topologiesWireless network protocolsStructure of Management InformationThe Internet
The invention discloses a thing Internet gateway of a heterogeneous wireless network and a control method thereof. Function, such as protocol conversion, route selection, data exchange and the like, is provided in the network interworking process of different structures or protocols of the thing Internet. The gateway is a multi-layer cascading gateway and comprises a main gateway used for communication, sensor network management and topology management, and sub-gateways responsible for converting and penetrating a short-range wireless protocol and receiving front-end sensing network data, wherein each of the sub-gateways is connected with the main gateway. The limitation to the access ability of a sensor network is overcome, so that various front-end sensing nodes access a thing Internet platform without barriers; free networking of the sensor network front end is realized by taking full advantage of the conventional communication capacity and cascading gateway technology at the same time; the problems of the failure management and performance management of the sensor network front end are effectively solved; and the safety, the reliability and the stability of the sensor network and neurones are enhanced.
Owner:天津中启创科技有限公司

Application gateway technology and system for realizing content interchange of Internet protocol version 4/Internet protocol version 6 (IPv4/IPv6) websites

InactiveCN102694754AQuick migrationAddressing the lack of appsNetwork connectionsDomain nameInternet content
The invention discloses an application gateway technology and an application gateway system for realizing content interchange of Internet protocol version 4 / Internet protocol version 6 (IPv4 / IPv6) websites, and relates to the field of data communication. A method for realizing the content interchange of the IPv4 / IPv6 websites comprises the following steps that: an application gateway is arranged between a cross-network user and a website, the user can perform cross-network hypertext transfer protocol (HTTP) access by the proxy of the gateway, and the gateway can access a data packet Payload to perform IPv4 / IPv6 conversion so as to help an IPv4 website rapidly to move to an IPv6 network; and an HTTP access request of the user is guided to a specified platform by modifying an IP address and a domain name to the form of using the domain name of an IPv6 application gateway as a father domain name, and an IPv4 user can initiate HTTP access to an IPv6 website. According to the technology and the method, an Internet content provider (ICP) can be assisted in moving the IPv4 website to the IPv6 network under the condition that an application provider does not update and modify the conventional system; and meanwhile, the IPv6 network is provided for the IPv4 user to access, and the problem of blank access due to network outlinks is solved.
Owner:睿哲科技股份有限公司

A vehicle network safety protection method based on Internet of Vehicles and a vehicle network architecture

InactiveCN109729056AImprove securityDefend against cyber attacksTransmissionElectronic controllerNetwork architecture
The invention provides a vehicle network safety protection method based on Internet of Vehicles and a vehicle network architecture. The method comprises the following steps: establishing a plurality of security defense layers for a whole vehicle network, wherein the plurality of security defense layers at least comprise an Internet of Vehicles defense layer, a whole vehicle electronic and electrical architecture defense layer, a vehicle-mounted network defense layer and an automobile electronic controller physical defense layer; For each security defense layer, selecting a preset security defense technology corresponding to the security defense layer to realize network security protection of the whole vehicle, wherein a firewall technology is correspondingly adopted by the Internet of Vehicles defense layer, and a central gateway technology is correspondingly adopted by the whole vehicle electronic and electrical architecture defense layer; Wherein the vehicle-mounted network defense layer correspondingly adopts a network message MAC encryption technology, and the automobile electronic controller physical defense layer correspondingly adopts an EVITA-based security hardware moduletechnology. The network attack can be effectively resisted, and the network security of the whole vehicle is improved.
Owner:CH AUTO TECH CORP CO LTD

CAN bus communication system

The present invention relates to a CAN bus communication system and discloses a bus type topological structure. In the specific technical proposal, the bus type topological structure of vehicle consists of a CAN bus and an LIN bus, and has five network channels which are connected through gateways so as to share the information. The five network channels comprise a high-speed CAN network channel which mainly consists of a power component and a chassis component, a low-speed CAN network channel which mainly consists of a vehicle body control display system and a meter sound display system, and three LIN network channels which mainly consist of a rainfall sensor, an anti-theft speaker, an auxiliary instrument, a left backdoor and a right backdoor. In the network topological structure with a plurality of channels and gateways, the network channels are arranged reasonably; the load rate of the network is controlled within a reasonable range; and the structure enhances the real-time performance of the network communication. Simultaneously, the adoption of the gateway technology realizes the management of the entire network and enhances the reliability of the network, when the information is shared.
Owner:CHERY AUTOMOBILE CO LTD

ZIGBEE transfer power line carrier wave gateway technology

The invention relates to a ZigBee transfer power line carrier wave gateway technology, consisting of a power carrier wave transmitting and receiving circuit, micro-processors and a coupling transformer, a data packet received from a power line communication terminal is transmitted to a micro-processor B by interfaces such as I2C, UART and the like located between a micro-processor A and the micro-processor B. ZigBee 2.4GHz transmitting and receiving circuit and the micro-processor B realize ZigBee protocol of ZigBee communication terminal, the user data packet transmitted from the micro-processor A is transmitted out by ZigBee communication, thus achieving the aim of the ZigBee transfer power line carrier wave gateway. The invention has the characteristics of simple application, long cell service life, networking capability, high reliability, low cost and the like.
Owner:许浩坤

Internal network architecture of hybrid electrical vehicle

The invention discloses an internal network architecture of a hybrid electrical vehicle. The internal network architecture comprises conventional power system nodes and electric drive system nodes. The conventional power system nodes are arranged on a high-speed CAN (controller area network) bus to form a conventional power system sub-network. The electric drive system nodes are arranged on another high-speed CAN bus to form an electric drive system sub-network. A gateway connected with the conventional power system sub-network and the electric drive system sub-network is a vehicle control unit of the hybrid electrical vehicle. By the internal network architecture, the two high-speed CAN buses with different functions are integrated in the same network by adopting a gateway technology, so that the internal network architecture is distinguished from a conventional single-channel network communication system. The dual-channel network reduces the load of a single-channel network and improves the real-time performance of each sub-network. Simultaneously, the security of the network is improved, namely control based on the sub-networks has the advantage that the other sub-network may not be influenced when one sub-network fails, information exchange is performed by the gateway to share information, and the characteristics of high efficiency and low cost of the CAN bus are fully utilized.
Owner:CHERY AUTOMOBILE CO LTD

Multi-gateway technology of intelligent housing system

The invention discloses a multi-gateway technology of an intelligent housing system. The multi-gateway technology of the invention comprises internet, a main router, a main gateway, sub-routers and sub-gateways. There is one main router and one main gateway only, and there are a plurality of sub-router and sub-gateways. The main router is connected with the main gateway and the internet, and the sub-routers are connected with the sub-gateways and the main router; the main gateway and the sub-gateways are connected via a network wiring system, and the network address must be the same to ensure the gateways to be on same network segment. The sub-routers and the main router are connected by using a concentrator. The multi-gateway technology of the invention has the beneficial effect of high-speed transmission, reliable transmission and unlimited transmission distance, and improves dynamic response performance of the intelligent housing system. The multi-gateway technology of the invention is widely applied to a control system of an intelligent home.
Owner:FOSHAN BOLANGDE TECH

Application of Arabidopis thaliana transcription factor at3g46080 gene

The invention relates to an application of an Arabidopis thaliana transcription factor at3g46080 gene. By constructing a CDS sequence of the Arabidopsis thaliana at3g46080 gene into the upstream of aFLAG protein label by a Gateway technology, Arabidopsis thaliana is conversed, which affects the disease phenotype of Arabidopsis thaliana, screening and identification are carried out, and finally the transgenic plants can be obtained. The analysis of the screened plants carried out so that the plant has enhanced resistance to Pseudomonas lycoposum by Arabidopis thaliana, the gene has the same function for homologous genes in crops, and thus has important significance in production.
Owner:XUZHOU NORMAL UNIVERSITY

Early warning emergency linkage mechanism implementation frame based on CAP (Common Alerting protocol)

The invention discloses an early warming emergency linkage mechanism implementation frame based on a CAP (Common Alerting protocol), which provides supports of system communication, data exchange and information aggregation based on distributed multi-channel early warning information issuing. On the base of CAP1.0 (Common Alerting protocol 01), the frame defines a data exchange format, a data communication encryption and decryption mode and a data collection and aggregation mode, provides conversion support for multiple early warning information content formats by adopting a converged communication gateway technology and realizes automatic conversion of information formats required by multiple issuing channels; due to adoption of a message queue technology, all information can be safely and reliably transmitted, and information issuing and monitoring measures can be provided. The usability, the monitorability and the manageability of all parts of an emergency linkage platform can be ensured trough a tcp / ip long connection mode; the communication security is guaranteed through a digital certificate encryption and decryption mode. The frame provides early warning support guarantee for weather, earthquake, public health and various other emergent events.
Owner:PCI TECH GRP CO LTD

Gene NtAOS1 for improving jasmonic acid content in tobacco leaves, cloning method and application of NtAOS1

ActiveCN110305884AIncrease jasmonic acid contentOxidoreductasesIsomerasesAgricultural scienceNicotiana tabacum
The invention discloses a gene NtAOS1 for improving jasmonic acid content in tobacco leaves, a cloning method and an application of the NtAOS1. The nucleotide sequence of the NtAOS1 which can improvethe content of jasmonic acid in tobacco is shown in SEQ ID NO. 1; the amino acid sequence encoding the protein is shown in SEQ ID NO. 2; a nucleic acid interference (RNAi) technology is used to designa segment of interference fragment, a gateway technology is used to construct plant expression vector and transform tobacco, and a qPCR technology is used to select NtAOS1 gene transcription level to significantly reduce transgenic plants. Detection of jasmonic acid content shows that the decreased expression level of the gene NtAOS1 results in significantly higher jasmonic acid content than that of wild-type control. The invention provides a material basis for applying the gene NtAOS1 and coded protein thereof to tobacco stress resistance breeding and subsequent research on jasmonic acid synthesis and metabolism pathway in tobacco.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI

Method for constructing multi-gene expression vector through combination of isocaudarner with Gateway clone technology

The invention discloses a method for constructing a multi-gene expression vector through combination of isocaudarner with a Gateway clone technology. The method comprises steps as follows: (1), an M35S-8GWN vector, an MNOS-8GWN vector and an MOCS-8GWN vector are constructed; (2), target genes are cloned, the genes are assembled to the M35S-8GWN vector, the MNOS-8GWN vector and the MOCS-8GWN vector through Not I and Sbf I respectively, and multiple gene expression cassettes are formed; (3), the multiple gene expression cassettes are connected in series through the isocaudarner AsiS I and Pac I; (4), the multiple gene expression cassettes are connected to the target expression vector through a Gateway LR reaction. The invention further discloses the three entry vectors M35S-8GWN, MNOS-8GWN and MOCS-8GWN used for constructing the multi-gene plant expression vector.
Owner:CHONGQING UNIV

Port mapping method and port mapping system under wide area network (WAN) environment

The invention provides a port mapping method and a port mapping system under a wide area network (WAN) environment. The port mapping method comprises steps that a, peripheral is inserted into a client side, and device information is encapsulated on a software service side and transmitted to a gateway; b, the gateway receives the device information transmitted by the client side and transmits the device information after being processed to a virtual desktop; and c, the virtual desktop receives the device information, loads a device drive, decompresses the device information and restores the device, so that the device mapping is successful. Due to the adoption of the port mapping method and the port mapping system under the WAN environment, through the principle that the peripheral transmission is analyzed under a traditional way, for the access of a peripheral device under the WAN environment, signal cheat is carried out for the device by utilizing an access gateway technology, the response time of the peripheral device is prolonged, the peripheral control information is cheated, and the peripheral access under the WAN situation is completed. Therefore, an effect for accessing the peripheral device under the WAN environment by utilizing a way for accessing the local peripheral device through a physical personal computer (PC) can be realized.
Owner:ISOFTSTONE INFORMATION TECHNOLOGY (GROUP) CO LTD

Gateway inlet vector pEn-L4*-PrbcS-*T-GFP-L3*, construction method thereof and application thereof

The invention relates to a gateway inlet vector for the gateway technology, and in particular to a gateway inlet plasmid vector (pEn-L4*-PrbcS-*T-GFP-L3*). The vector comprises sequences L4 and L3, rubisco, a small subunit gene photo-inductive promoter (PrbcS), a chloroplast stroma positioning sequence (T*) and a green fluorescent protein (GFP) reporter gene, which are required for the LR recombination reaction in the gateway technology. A construction method and application of the vector are that: one plant expression vector connected in series with two target gene expression boxes can be quickly constructed by using the vector and another gateway inlet vector containing L1 and L2 sequences through the LR recombination reaction so as to realize conversion operation of two target genes through one conversion event. The expression of the target gene can be controlled with the PrbcS promoter in the vector and high-level expression of the target gene in the leaves of a plant can be realized by substituting the GFP gene in the vector with the target gene.
Owner:KUNMING UNIV OF SCI & TECH

Broad host range plasmid carrying xylose metabolism related gene and construction method thereof

The invention discloses a wide host plasmid carrying xylose metabolism related genes and construction method thereof, belonging to the field of molecular biology techniques. The plasmid contains four xylose metabolism related enzyme genes, xylose isomerase (xylA), xylulokinase (xylB), transaldolase (talA) and transketolase (tktA), which are derived from E.coli DH5alpaha strain. The plasmid construction method uses the Gateway technology, to respectively design specific primers of four target genes xylA, xylB, talA and tktA, takes the E.coli DH5alpaha strain as the origin stain of the target genes to amplify the corresponding gene through the PCR; the PCR product performs BP recombination with the corresponding pDONR vector, and then the obtained Entry clones perform LR recombination with the pDEST14, to obtain the pDEST14-ABAT plasmid; the pDEST14-ABAT plasmid is connected to the Xha I single zyme cutting vector pBBR1MCS2 through the Xba I and Nhe I double zyme cutting product to obtained the recombinant plasmid pBBR1MCS2-ABAT. The superior strain shifted in the plasmid can greatly reduce production costs of cellulosic ethanol.
Owner:TSINGHUA UNIV

shRNA for inhibiting mouse TRAF6 gene expression and application thereof

The invention discloses shRNA for inhibiting mouse TRAF6 gene expression and application thereof. The deoxyribonucleic acid (DNA) sequence of the shRNA for inhibiting the mouse TRAF6 gene expression is GAATCACTTGGCACGACACTT. The shRNA for inhibiting the mouse TRAF6 gene expression is prepared by the following steps of: designing the DNA sequence which contains the shRNA for inhibiting the mouse TRAF6; cloning the DNA sequence to a pENTR / U6 vector; and transferring the DNA sequence which is positioned on a recombinant vector pENTR / U6-TRAF6-shRNA by Gateway technology to obtain CS-RfA-EG-TRAF6-shRNA recombinant plasmids which can totally inhibit the expression of the mouse TRAF6 gene. Therefore, the recombinant plasmids are extremely significant to research on the effect of the TRAF6 and the treatment of diseases caused by the TRAF6.
Owner:JINAN UNIVERSITY

Plant RNAi expression vector and construction method and application thereof

The invention belongs to the technical field of genetic engineering, and particularly relates to a plant RNAi expression vector and a construction method and application thereof. The plant RNAi expression vector containing a CaMV35S promoter, an intron, an OCS terminator, and two opposite-direction Gateway boxes is constructed based on commercial plant expression vectors pCambia2301 and pHANNIBIAL. The plant RNAi expression vector can be used for construction of a plant gene silencing vector by using Gateway technology; the present invention also establishes the construction method of the plant RNAi expression vector and application of the plant RNAi expression vector in constructing of a target-gene-containing RNAi silencing vector by use of fusion PCR for a one-step Gateway reaction.
Owner:ZHOUKOU NORMAL UNIV

Application of arabidopsis transcription factor AT3G46090 gene in cultivation of disease-resistant transgenic plants

The invention provides the application of an Arabidopsis thaliana transcription factor, namely the AT3G46090 gene in cultivation of disease-resistant transgenic plants. The CDS sequence of the Arabidopsis thaliana AT3G46090 gene is constructed to upper stream with an FLAG protein tag through the Gateway technology, Arabidopsis thaliana plants are transformed, screened and identified, and finally, the transgenic plants are obtained. The screened plants are analyzed, and it is found that the AT3G46090 gene has the effect of enhancing the resistance of the plants to the pseudomonas syringae tomato subspecies, the expression of the gene affects the disease resistance phenotype of the plants, and homologous genes in crops possibly have the same function, so the gene has important significance in production.
Owner:XUZHOU NORMAL UNIVERSITY

Application modular integration method and device

The invention discloses an application modular integration method and device, and relates to the technical field of computers. A specific embodiment of the method comprises the steps of receiving a data transmission request, wherein the data transmission request comprises a URL path; determining an application example according to the URL path and a preset configuration strategy matched with the URL path; and sending the data transmission request to the application instance through a gateway. The implementation mode is based on the gateway technology and the configuration strategy, forwardingof the HTTP request can be controlled in a fine-grained mode, modular integration of large Web applications can be achieved, the Web applications provide services through the gateway, the Web applications are called in multiple pages, the cross-domain problem does not exist, and the cross-domain data sharing problem of an existing system is solved. And meanwhile, development, testing and deployment can be performed by taking a single page as a minimum unit, that is, the page is an integrated minimum unit, independent deployment of each page is supported, and faults can be well isolated.
Owner:BEIJING JINGDONG SHANGKE INFORMATION TECH CO LTD +1

Intelligent electric furnace combining internet-of-things technology and mobile management application

The invention discloses an intelligent electric furnace combining an internet-of-things technology and an mobile management application. The intelligent electric furnace comprises an electric furnace body, a data acquisition system, an automatic data transmission system, a data receiving system, an equipment manager background and a user operation system. The data acquisition system comprises a temperature sensor and a signal output module, a pressure sensor and a signal output module, a flow sensor and a signal output module, a water quality sensor and a signal output module, a voltage mutual inductor and a current mutual inductor, or meter values are manually input in a regularly timed manner; the automatic data transmission system comprises a signal conversion device and an automatic internet transmission technology based on an intelligent gateway technology; the data receiving system comprises a data receiving server and a background data processing device; the equipment manager background is used for analyzing and processing data; the user operation system has the automatic equipment diagnosis function, the regular diagnosis or spot check information management function and the equipment maintenance, repair and management functions.
Owner:昆山良昕环保节能有限公司

Application of arabidopsis transcription factor AT5G59820 gene in cultivation of disease-resistant transgenic plants

The invention relates to an application of arabidopsis thaliana transcription factor AT5G59820 gene in cultivation of disease-resistant transgenic plants, the CDS sequence of the arabidopsis thaliana transcription factor AT5G59820 gene is constructed to the upstream with an FLAG protein tag through the Gateway technology, the arabidopsis thaliana plant is transformed, screened and identified, and finally the transgenic plants are obtained. The screened plants are analyzed and found to have the effect of enhancing the resistance of arabidopsis thaliana to tomato pseudomonas syringae subspecies, that is, the gene affects the disease resistance phenotype of the plants, and homologous genes in crops possibly have the same or similar functions, so that the gene has important significance in production.
Owner:XUZHOU NORMAL UNIVERSITY

Information transmission optimization method applicable to large-scale distributed joint simulation

ActiveCN105740045ASolve the bottleneck problem of reduced operating efficiencyReduce loadData switching networksSoftware simulation/interpretation/emulationInformation transmissionCoordinate time
The present invention discloses an information transmission optimization method applicable to large-scale distributed joint simulation. The information transmission optimization method comprises the following steps: S1: generating a gateway configuration initialization file by using a multi-federation configuration module; S2: generating a multi-cast address mapping table by using a communication multi-cast address management module; and S3: coordinating time advances of federations by using a time-unified module. The outstanding characteristics of the information transmission optimization method are as follows: a multi-federation architecture and a highly extensible HLA gateway technology are adopted; and by generating a multi-federation unified multi-cast address mapping table, the gateway gets rid of the function of inter-federation information communication, and the load is reduced. Moreover, by establishing a special time-unified module, the function of multi-cast simulation time coordination is completed.
Owner:BEIJING SIMULATION CENT

A multimedia communication safe proxy gateway and safety proxy method

The present invention relates to safety agent gateway and safety agent method for multimedia communication and combined with fire wall technology, and the multimedia communication penetrates the fire wall without degrading the safety of fire wall. The safety agent gateway includes H.323 protocol control module, data relay module, safety control module and network keeper module, and is located in the joint between the inner network and outer network and with fire wall set. The safety agent method includes the agent module and network keeper module linking technology; the automatically sensing safety agent gateway technology and the medium routing technology.
Owner:西安西邮双维通信技术有限公司

A hybrid vehicle internal network architecture

The invention discloses an internal network architecture of a hybrid electrical vehicle. The internal network architecture comprises conventional power system nodes and electric drive system nodes. The conventional power system nodes are arranged on a high-speed CAN (controller area network) bus to form a conventional power system sub-network. The electric drive system nodes are arranged on another high-speed CAN bus to form an electric drive system sub-network. A gateway connected with the conventional power system sub-network and the electric drive system sub-network is a vehicle control unit of the hybrid electrical vehicle. By the internal network architecture, the two high-speed CAN buses with different functions are integrated in the same network by adopting a gateway technology, so that the internal network architecture is distinguished from a conventional single-channel network communication system. The dual-channel network reduces the load of a single-channel network and improves the real-time performance of each sub-network. Simultaneously, the security of the network is improved, namely control based on the sub-networks has the advantage that the other sub-network may not be influenced when one sub-network fails, information exchange is performed by the gateway to share information, and the characteristics of high efficiency and low cost of the CAN bus are fully utilized.
Owner:CHERY AUTOMOBILE CO LTD

Bluetooth mesh multi-gateway local and remote control coexistence method and device

In order to solve the problem that in the prior art, a Bluetooth mesh multi-gateway technology can easily generate conflict during the local control and remote control coexistence, the invention provides a Bluetooth mesh multi-gateway local and remote control coexistence method and device. The method comprises the following steps of obtaining instruction information and instruction serial number issued by locality or gateways; judging whether the equipment connection state simultaneously has local connection and remote gateway connection or not; if so, shielding the instruction information issued by the gateway by equipment according to the issued instruction serial number; processing the instruction information issued by the locality.
Owner:HANGZHOU TUYA INFORMATION TECH CO LTD

TYLCV and ToCV bivalent RNAi expression vector established through Gateway technology and application of expression vector

The invention discloses a TYLCV and ToCV bivalent RNAi expression vector established through a Gateway technology and application of the expression vector. The bivalent RNAi expression vector is namedas pRNAi-TOCV-TY. A nucleotide sequence of the RNAi expression vector is shown in SEQ ID NO.5. According to the expression vector, an RNAi technology is used for a lot of research work, through the Gateway technology, the TYLCV and ToCV coat protein gene bivalent RNAi expression vector is established, and through experiment methods such as genetic transformation and resistance identification, a transgenic lycopersicon esculentum Mill. plant resisting the two viruses is obtained. The invention further discloses application of the pRNAi-TOCV-TY expression vector in effective prevention and control over lycopersicon esculentum Mill. with composite infection of the ToCV and TYLCV, particularly application of the pRNAi-TOCV-TY expression vector in effective obstruction for lycopersicon esculentum Mill. virus replication in plant bodies.
Owner:天津市农业科学院
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