9 results about "Human fibrinogen" patented technology

The application discloses a human fibrinogen high-efficiency separation device and relates to the technical field of biological medicine processing. The device comprises a tank body, a stirring mechanism and a conveying mechanism. The stirring mechanism comprises a stirring shaft and a stirring paddle. The conveying mechanism comprises a fluid channel and a liquid outlet. The stirring paddle is internally provided with a mounting cavity in communication with the fluid channel and the liquid outlet. A sliding part is slidably arranged in the mounting cavity. The device further comprises a magnetic driving assembly for driving the sliding part to reciprocatingly slide in the mounting cavity so as to extrude and spray the fluid from the liquid outlet. In the application, when the stirring paddle rotates to a specific position, the sliding part extrudes the mounting cavity under the magnetic attraction force of the permanent magnet, so that the pre-cooled ethanol is rapidly sprayed from the liquid outlet. The spraying mode makes the diffusion range of the pre-cooled ethanol in the blood plasma larger and the diffusion speed faster than those of the traditional mode, thereby avoiding the instantaneous concentration of the pre-cooled ethanol in the local part being too high, effectively preventing the denaturation of the protein in the blood plasma, avoiding the local ethanol concentration being too high and preventing the protein denaturation.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

The invention belongs to the technical field of blood product preparation, and particularly relates to a method for filtering, separating and purifying human fibrinogen from plasma. The method sequentially comprises the following steps: balancing cellulose, mixing the balanced cellulose with raw material plasma, adsorbing and filtering to obtain cellulose precipitate; leaching the cellulose precipitate, soaking the cellulose precipitate in the precipitate dissolving solution, and filtering to obtain filtrate; carrying out first ion exchange chromatography on the filtrate; and carrying out secondary ion exchange chromatography to obtain a protein solution with fibrinogen purity of more than 80%. According to the technical scheme, the technical problems that in the prior art, the process steps of purifying and enriching the human fibrinogen from the plasma cryoprecipitate are tedious, and the utilization rate of the human fibrinogen is not ideal can be solved. According to the cryoprecipitated protein solution prepared by the method, VIII factor, vWF factor and fibrinogen products can be separated at the same time through chromatography, the utilization rate of plasma protein is improved, and the method has ideal popularization and application prospects.

The utility model relates to the technical field of human fibrinogen production, and discloses a centrifugal machine for human fibrinogen production, which comprises a machine body shell, a top cover hinged to one side of the machine body shell, a circular partition plate fixedly connected to the inner side of the machine body shell, and a driving motor fixedly mounted on the inner bottom wall of the machine body shell, the output end of the driving motor is fixedly connected with a driving rod, the top end of the driving rod vertically and upwards penetrates through the circular partition plate, the outer wall of the top of the driving rod is fixedly sleeved with a supporting plate, the outer wall of the driving rod is sleeved with a connecting block, the connecting block is arranged above the supporting plate, and the top of the driving rod is in threaded sleeving with a nut used for fixing the connecting block. A plurality of placing assemblies used for placing centrifugal tubes are fixed to the outer wall of the connecting block in an annular array mode, through cooperation of the clamping fixing assemblies and the placing assemblies, the device can conduct centrifugal operation on the test tubes in the inclined state and the vertical state at the same time, and the device can meet various centrifugal requirements through the design.

The invention relates to a preparation process for extracting human fibrinogen from a component I, and belongs to the technical field of blood products. The process comprises the following steps: dissolving an FI precipitate in a dissolving solution A containing sodium citrate, lysine hydrochloride and heparin sodium, and filtering to obtain a filtrate A; adding PEG (Polyethylene Glycol) into the filtrate A until the final concentration is 5%, purifying and separating, collecting PEG precipitate, dissolving and filtering to obtain filtrate B; adding polysorbate 80 and tributyl phosphate into the filtrate B to carry out S / D inactivation; cooling the inactivated liquid, adding an ethanol solution for purification and separation, collecting the precipitate, and dissolving to obtain a filtrate C; adding glycine into the filtrate C until the final concentration is 1.8 mol / L, performing purification and separation, collecting precipitates, and dissolving to obtain filtrate D; performing ultrafiltration dialysis on the filtrate D to obtain a stock solution; and diluting the stock solution to a proper concentration, sterilizing, filtering, sub-packaging, freeze-drying, and carrying out dry heat inactivation treatment to obtain a finished product. The method is simple in process and low in cost, and the prepared human fibrinogen is high in purity, good in safety and suitable for clinical hemostasis and tissue repair.

A preparation method for human fibrinogen, in which a component I precipitate is used as a raw material, a specific solvent is used in combination with a low-temperature ethanol method to prepare a human fibrinogen bulk solution, and virus inactivation is performed by combining an S / D virus inactivation method and a dry heat virus inactivation method, thereby further guaranteeing the safety of the human fibrinogen. The prepared human fibrinogen has high purity and high reconstitution speed, a reconstitution solution is clear and only slightly opalescent, and the coagulation time is short.

The utility model relates to the technical field of stirring devices, and discloses a stirring device suitable for human fibrinogen, which comprises a stirring box, the top end of a box cover is fixedly connected with a plurality of connecting frames, a circular plate is fixedly connected between the bottom ends of the connecting frames, a scraping assembly is arranged from the top end to the bottom end of the box cover, and the bottom end of the scraping assembly is provided with a scraping plate. A stirring assembly is arranged between the top end and the bottom end of the circular plate, two cleaning assemblies are arranged in the stirring assembly, a telescopic assembly is arranged at the top end of the stirring assembly, and a transmission assembly is arranged between the top end of the box cover and the top end of the circular plate; according to the human fibrinogen stirring device, the scraping assembly, the stirring assembly, the cleaning assembly, the telescopic assembly and the transmission assembly are arranged in a matched manner, so that water spraying, scraping and cleaning can be conveniently carried out in the stirring box, the human fibrinogen stirring device has various functions, and the interior of the stirring device can be conveniently cleaned in time before subsequent use; therefore, the problems of cross contamination, microbial contamination and the like are not easy to occur.

The invention belongs to the technical field of glycoprotein, and particularly relates to a method for preparing human fibrinogen. The method comprises the following steps: by taking a low-temperature ethanol method component I as a raw material, carrying out PEG (Polyethylene Glycol) precipitation one-step purification to enable the product to reach 90% or more, carrying out S / D virus inactivation, removing an S / D inactivator by utilizing chromatographic separation, replacing salt in an eluent by utilizing a dialysis buffer solution, degerming, freeze-drying and carrying out dry heat inactivation to obtain a finished product. Mild PEG precipitation separation is adopted for crude preparation of the product, compared with a traditional low-temperature ethanol method, the change of a protein structure caused by ethanol precipitation is not needed, the molecular structure tends to be natural, detection shows that the redissolution time of the product is within 5 min, the purity reaches up to 90% or above, and the product can be used for clinical blood coagulation and can also be used for a component of blood coagulation gel.

The application provides a fibrinogen precipitation device and a purification process. The fibrinogen precipitation device comprises a shell, a driving device and a feed pipe. A liquid storage cavity is arranged in the shell to accommodate a first liquid containing fibrinogen. The driving device is connected to the shell to ensure that the first liquid is in a flowing state. The feed pipe is arranged through the shell to deliver a second liquid. The feed pipe comprises an extension arranged obliquely. The extension is partially inserted into the first liquid. An angle alpha is formed between the direction of the extension and the flowing direction of the first liquid flowing through the feed pipe, wherein 0 < alpha < 90 degrees. The fibrinogen precipitation device can significantly reduce the proportion of precipitate A generated after the addition of ethanol, reduce protein denaturation to improve product stability, significantly reduce the residual amount of inactivator in the product, and be beneficial to the control of the impurity content of human fibrinogen process.