2 results about "Human fibrinogen" patented technology

The application discloses a human fibrinogen high-efficiency separation device and relates to the technical field of biological medicine processing. The device comprises a tank body, a stirring mechanism and a conveying mechanism. The stirring mechanism comprises a stirring shaft and a stirring paddle. The conveying mechanism comprises a fluid channel and a liquid outlet. The stirring paddle is internally provided with a mounting cavity in communication with the fluid channel and the liquid outlet. A sliding part is slidably arranged in the mounting cavity. The device further comprises a magnetic driving assembly for driving the sliding part to reciprocatingly slide in the mounting cavity so as to extrude and spray the fluid from the liquid outlet. In the application, when the stirring paddle rotates to a specific position, the sliding part extrudes the mounting cavity under the magnetic attraction force of the permanent magnet, so that the pre-cooled ethanol is rapidly sprayed from the liquid outlet. The spraying mode makes the diffusion range of the pre-cooled ethanol in the blood plasma larger and the diffusion speed faster than those of the traditional mode, thereby avoiding the instantaneous concentration of the pre-cooled ethanol in the local part being too high, effectively preventing the denaturation of the protein in the blood plasma, avoiding the local ethanol concentration being too high and preventing the protein denaturation.

The application provides a fibrinogen precipitation device and a purification process. The fibrinogen precipitation device comprises a shell, a driving device and a feed pipe. A liquid storage cavity is arranged in the shell to accommodate a first liquid containing fibrinogen. The driving device is connected to the shell to ensure that the first liquid is in a flowing state. The feed pipe is arranged through the shell to deliver a second liquid. The feed pipe comprises an extension arranged obliquely. The extension is partially inserted into the first liquid. An angle alpha is formed between the direction of the extension and the flowing direction of the first liquid flowing through the feed pipe, wherein 0 < alpha < 90 degrees. The fibrinogen precipitation device can significantly reduce the proportion of precipitate A generated after the addition of ethanol, reduce protein denaturation to improve product stability, significantly reduce the residual amount of inactivator in the product, and be beneficial to the control of the impurity content of human fibrinogen process.