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Adhesion agent for fixing articular cartilage repair implant and use of human fibrinogen in preparing the adhesion agent

A technology of human fibrinogen and articular cartilage, applied in the field of human-derived biological protein, to achieve the effect of good biocompatibility and simple operation technology

Inactive Publication Date: 2009-01-28
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no clinical reports and studies on fixing different materials (such as biofilm or tissue engineering cartilage and other grafts) by adjusting the different solidification speeds of human fibrinogen for articular cartilage repair.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, for the fixation of tissue engineered cartilage on the surface of cartilage defect in the joint cavity

[0017] ①The tissue engineering scaffold material should be washed with normal saline or other liquids, and the water should be sucked out as much as possible, immersed in human fibrinogen solution (0.2-10mg / ml) for 1-60min, and the excess liquid should be sucked off with sterile gauze;

[0018] ②Digest and adjust the cells to be transplanted, and add thrombin (2-100U / ml) in PBS liquid according to an appropriate ratio (such as 9:1 volume ratio) to form a cell suspension;

[0019] ③In the container, drop the cell suspension into the scaffold material, and then quickly transplant it to the surface of the cartilage defect in the joint cavity to evenly fill the defect area;

[0020] ④ Then drop a small amount of 50mM CaCl 2 The solution promotes the formation of biological protein gel; the whole pasting process is completed after about 10 minutes.

[0021...

Embodiment 2

[0023] Embodiment 2, for the fixation of biomaterial film on the surface of cartilage defect in the joint cavity

[0024] ①Biological material film is washed with normal saline or other liquids, suck out the water as much as possible, immerse in human fibrinogen solution (0.2-10mg / ml) for 1-60min, and absorb the excess liquid with sterile gauze;

[0025] ②According to the area and shape of the cartilage defect, trim the thin film, transplant it to the surface of the cartilage defect in the joint cavity, and evenly fill the defect area;

[0026] ③ Use a syringe with a long needle to mix thrombin (10-50U / ml) and 50mM CaCl in saline or other liquids 2 The solution is added dropwise to the interface between the film material and the internal cartilage defect to maintain a good position of the biomaterial film, and the pasting process is completed after about 1-5 minutes.

[0027] ④Move the joints repeatedly, observe that the graft does not fall off, touch the graft lightly with t...

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PUM

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Abstract

The invention discloses an adhesive used for fixing articular cartilage repairing graft, which essentially comprises human fibrinogen and thrombin used as a coagulator. The adhesive has the advantages of simple fixing technique in repairing operation, good biological compatibility and easy absorption.

Description

technical field [0001] The invention relates to a new application of human biological protein, which is used as an adhesive for fixing grafts in the treatment of cartilage defects. Background technique [0002] In the treatment of articular cartilage defects, some new grafts such as biomaterial films or tissue engineered cartilage can be transplanted on the surface of cartilage defects for repair, but there is a defect that it is difficult to fix the grafts. If arthroscopic techniques under small incisions are used, then It is difficult to suture and fix, and if open surgery is used for incision and fixation, there will be a large surgical trauma. Moreover, if the implanted fixture cannot degrade by itself and be absorbed by the surrounding tissue, it needs to be removed by surgery. [0003] Human Fibrinogen (Human Fibrinogen), also known as fibrinogen, human fibrinogen, human fibrinogen for injection, is obtained from the plasma of healthy people. Coagulation disorders ca...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/02
Inventor 段小军杨柳戴刚郭林陈光兴
Owner ARMY MEDICAL UNIV
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