79 results about "Articular cartilage repair" patented technology

Devices formed of or including biocompatible polyhydroxyalkanoates are provided with controlled degradation rates, preferably less than one year under physiological conditions. Preferred devices include sutures, suture fasteners, meniscus repair devices, rivets, tacks, staples, screws (including interference screws), bone plates and bone plating systems, surgical mesh, repair patches, slings, cardiovascular patches, orthopedic pins (including bone filling augmentation material), adhesion barriers, stents, guided tissue repair/regeneration devices, articular cartilage repair devices, nerve guides, tendon repair devices, atrial septal defect repair devices, pericardial patches, bulking and filling agents, vein valves, bone marrow scaffolds, meniscus regeneration devices, ligament and tendon grafts, ocular cell implants, spinal fusion cages, skin substitutes, dural substitutes, bone graft substitutes, bone dowels, wound dressings, and hemostats. The polyhydroxyalkanoates can contain additives, be formed of mixtures of monomers or include pendant groups or modifications in their backbones, or can be chemically modified, all to alter the degradation rates. The polyhydroxyalkanoate compositions also provide favorable mechanical properties, biocompatibility, and degradation times within desirable time frames under physiological conditions.

A technique for the arthroscopic delivery and fixation of an articular cartilage repair device or implant is provided. The technique includes the use of a cannula tube that functions as both a cartilage cutter and a guide to pass instruments into the body arthroscopically. One such instrument is an end-cutting reamer that both prepares the subchondral bone by re-surfacing it down to a specified depth and also simultaneously drills a pilot hole in the subchondral bone to accept the cartilage repair device. A delivery device is utilized to hold and deliver the cartilage repair device to the delivery site.

The invention discloses a preparing method for infrared response high-strength hydrogel for cartilago articularis repair. The preparing method mainly includes the operation steps that polysaccharide or protein is modified in a double bond forming mode through methacrylic acid, methacrylic anhydride and methacrylate substances, so that the polysaccharide or the protein has a double-bond group in a side chain and is capable of being polymerized to form the hydrogel. Under the action of an initiator and a cross-linking agent, N-isopropyl acrylamide and the polysaccharide or the protein modified in the double bond forming mode form the double-network high-strength hydrogel through in-situ polymerization. The prepared infrared response high-strength hydrogel is high in biocompatibility and excellent in mechanical property and has good application prospects in the field of cartilago articularis repair.

The invention provides II-type collagen joint cartilage fluid and a preparation method thereof. Bacteria, viruses and other hazardous substances of the joint cartilage are removed through degreasing-> serum removing-> surfactant treatment-> oxidant treatment-> disinfection and other links. Hybrid protein and other denatured protein of the cartilage are removed through acid hydrolysis, salt precipitation, dialysis and other processes, and high-purity and high-activity II-type collagen solution is prepared. Non-toxin and non-heat raw materials and auxiliary materials and isotonic solution are mixed in an appropriate ratio, and filled in a sterile non-heat disposable injector, to prepare II-type collagen joint cartilage fluid for disposable injection. Other components of the II-type collagen joint cartilage fluid comprise the auxiliary materials and the isotonic solution. The II-type collagen joint cartilage fluid can play the roles of lubrication, pain relieving and joint activity degree improving to the injured joints, fundamentally realizes the internal repair to the cartilage tissue, and is applicable to a patient with various joint cartilage injuries and all types of arthritis.

The invention discloses a method for preparing an ultra-thin porous lamination gradient composite support of tissue engineering, belonging to the field of biomedicine material. The preparation method comprises the following steps: (1) determining materials and structures of a support to be prepared according to the condition of repair and defection; (2) preparing each layer of the support, waiting for the volatilization of a solvent, and gradually drying slurry for forming; (3) placing a formed object to a ventilation place, and demoulding the formed object after the residual solvent is completely volatilized; and (4) taking out the formed object and dry to obtain the ultra-thin porous lamination gradient composite support of the tissue engineering. The ultra-thin porous lamination composite support, the thickness of which is suitable for articular cartilage repair, can be prepared by using the method. The prepared support has favorable mechanical property, favorable biocompatibility and controllable degradation rate, is suitable for co-culture of osteoblast and chondrocyte and compound of specific growth factors, and can repair the synthetic defection of the joint cartilage tissue and the cartilage/bone tissue.

Disclosed is a layered bionic joint cartilage restoring and replacing material and the preparation method thereof. The material is a composite material of nanometer hydroxyl apatite with layered structure and polyvinyl alcohol; the layered structure means that the nanometer hydroxyl apatite content in different layer is different, and reduces in order from the bottom layer to the surface lafyer. The invention causes the nanometer hydroxyl apatite with biological activity to distribute in composite material by gradient along the thickness direction; realizes the optimization of the biological mechanical properties and the biology tribological properties of the joint cartilage restoring material, while, can effectively improve the connection strength of the implantation material and the bottom layer natural joint cartilage; and really solves the contradiction between the biological mechanical properties and the biology tribological properties of the artificial joint cartilage restoring material, and the fixing and combination strength of the implantation material and the natural body.

The invention provides an integrated three-layer composite scaffold for repairing joint cartilages, and a production method thereof. The composite scaffold comprises a cartilage layer, a calcified subchondral bone plate layer and a spongy bone layer which are sequentially superposed from bottom to top, the composite scaffold is formed in a one-shot manner through three-dimensional printing, the cartilage layer is a sodium alginate hydrogel porous layer, the calcified subchondral bone plate layer is a biological ceramic particle and sodium alginate composite hydrogel compact layer, the spongy bone layer is a biological ceramic particle and sodium alginate composite hydrogel porous layer, and the sodium alginate components in all above layers undergo ionic cross-linking to form an integrated structure, so every layer of the composite scaffold has a specific composition and a specific structure, the composite scaffold has good interlayer binding force, and the integral structure stability of the composite scaffold is improved. The invention also provides the production method of the integrated three-layer composite scaffold for repairing joint cartilages. The method has the advantages of simplicity, easiness in enforcement, easiness in control of technologic parameters, acceleration of the promotion and application of a three-dimensional printing technology in the biologic field, and wide market prospect.

The invention belongs to the field of articular cartilage repair materials, and provides a preparation method and application of high strength and porous structure polyvinyl alcohol-gallotannic acid hydrogel. The hydrogel is a potential articular cartilage replacement material. According to the preparation method, polyvinyl alcohol is adopted as a substrate material, the ice crystal forming ability of a hydrogel precursor solution is improved by introducing gallotannic acid and microbubbles, and meanwhile, the mechanical property of the hydrogel is greatly enhanced due to the intermolecular interaction between the gallotannic acid and the polyvinyl alcohol. The porous structure and high strength hydrogel is prepared at one step, the preparation process is simple, repeatability is high, theobtained hydrogel has good biocompatibility, meets a porous environment for adhesion and proliferation of cartilage cells and the mechanical property similar to natural articular cartilage, and can be applied to the field of articular cartilage replacement materials.

The present invention relates to a preparation method and uses of a composite hydrogel containing hydroxyapatite (HAP) nanoparticles, wherein an inorganic component in the composite hydrogel is HAP nanoparticles prepared in an in situ manner, an organic matrix in the composite hydrogel is an overoxidation micelle (pMIC) polyacrylamide gel prepared by using a pre-irradiation method, an overoxidation linear polymer chain polyacrylamide gel or a polyvinyl alcohol gel, and a mass ratio of the HAP to the polymer in the composite hydrogen is 1:5-1:3. According to the present invention, a freezing-defrosting circulation method is adopted to treat a gel, and an electrophoresis method is adopted to generate HAP nanoparticles inside the gel in an in situ manner, such that the HAP nanoparticles are uniformly dispersed in the gel, and HAP having a hollow sphere structure is obtained so as to enhance mechanical property and improve biological activity, such that potential application values in articular cartilage repair and tissue engineering materials are provided.

The invention discloses an adhesive used for fixing articular cartilage repairing graft, which essentially comprises human fibrinogen and thrombin used as a coagulator. The adhesive has the advantages of simple fixing technique in repairing operation, good biological compatibility and easy absorption.

An implant is configured for repair and regeneration of cartilage lesions. The implant includes a three-dimensional woven textile scaffold and a three-dimensional rigid, porous substrate. The scaffoldis bonded to the substrate such that the substrate supports the scaffold. The substrate is configured to be inserted into bone tissue including cartilage lesions such that the substrate and the scaffold replace the bone tissue including the cartilage lesions. The substrate and scaffold are further configured such that after the substrate and the scaffold have replaced the bone tissue including the cartilage lesions, the substrate and scaffold promote growth and integration of new bone tissue into the implant.

The invention discloses a preparation method of a mineralized bacterial cellulose/polyvinyl alcohol composite aquagel cartilage repairing material. The preparation method comprises the following steps of: carrying out purification treatment and dehydration and immersion treatment on bacterial cellulose to obtain a bacterial cellulose aquagel, wherein the bacterial cellulose is obtained through fermental cultivation of a bacterial strain, and the bacterial cellulose aquagel contains 30-50wt% of CaCl2 water solution; dissolving polyvinyl alcohol into an Na2HPO4 water solution to obtain a mixed solution A; and enabling the mixed solution A to enter the inside of the bacterial cellulose aquagel by using an immersion method to obtain a product, freezing and unfreezing the product for multiple times, and then, carrying out dehydration treatment in vacuum for 6-24 hours to obtain the mineralized bacterial cellulose/polyvinyl alcohol composite aquagel cartilage repairing material. The preparation method is simple in process, low in cost and free of pollution; and the prepared composite aquagel has favorable mechanical property, chemical stability and biocompatibility and has wide application prospect in articular cartilage repair, meniscus repair and other cartilage repair.

The invention discloses near-infrared response hyaluronic acid hydrogel for articular cartilage repair and a preparation method of the near-infrared response hyaluronic acid hydrogel. The hydrogel is prepared from azobenzene derivative modified hyaluronic acid and cyclodextrin derivative modified hyaluronic acid as raw materials through a cross-linking reaction according to the mass ratio of 1: 1. The azobenzene derivative is 4-o-methoxy substituted azobenzene modified by a long alkyl chain, and the cyclodextrin derivative is cyclodextrin modified by hexamethylenediamine. The azobenzene derivative modified hyaluronic acid and the cyclodextrin derivative modified hyaluronic acid are dissolved in proportion, the two solutions are evenly mixed, and standing or ultrasonic gel forming is conducted. The hydrogel has self-healing property and injectable property based on non-covalent cross-linked hydrogel, and can be subjected to partial gel-sol conversion under the stimulation of near-infrared light, so that the density of a cross-linked network on the surface of the gel is reduced to form a hydration layer, and due to the mechanics based on near-infrared light response and the formation of the hydration layer, therefore, the product has potential significance in the field of cartilage repair.

The invention discloses a mechanical stimulation method for improving the in-vitro preservation effect of articular cartilage. The method comprises the steps as follows: articular cartilage tissue ofanimals or human is acquired sterilely in vitro; the articular cartilage tissue is preserved in a tissue culture solution under certain pressure, humidity and air temperature, cartilage transplant blocks are subjected to periodical mechanical stimulation every 3 days with a special rolling mechanical stimulation method according to the parameter combination (0.1-3 HZ, 1-120 min and 0.1-3 Mpa) of stimulation frequency, time and pressure intensity, and then, the culture solution is replaced for preservation; the cell survival rate and Young modulus of cartilage tissue are detected in different periods. By means of the method, the cell survival rate and mechanical property of the cartilage tissue are notably improved, the duration of the action is significantly prolonged, and the in-vitro preservation effect of the cartilage tissue is improved. The method can be applied to cartilage tissue culture preservation tissue banks, and the utilization rate of cartilage tissue banks and the repairquality of articular cartilage are significantly improved.

The invention discloses a PLGA cytoskeleton for articular cartilage repair and a preparation method and application of the PLGA cytoskeleton for articular cartilage repair. The PLGA cytoskeleton comprises a PLGA porous scaffold, cartilage cells, mesenchymal stem cells and adipose-derived stem cells, and the cartilage cells, the mesenchymal stem cells and the adipose-derived stem cells are plantedin the PLGA porous scaffold. The PLGA porous scaffold is a hierarchical composite scaffold formed by PLA (polylactic acid) and PGA (polyglycolic acid) polymerization products in different polymerization ratios and comprises three areas including an outside area, a middle area and an inside area. The cartilage cells are subjected to injection inoculation in pores of the outside area of the PLGA porous scaffold; the cartilage cells and the adipose-derived stem cells are subjected to injection inoculation in pores of the middle area; the cartilage cells and the mesenchymal stem cells are subjected to injection inoculation in pores of the inside area of the PLGA porous scaffold. By combination of a gradient-ratio artificially synthesized high polymer material and natural biological protein, ahierarchical composite structure is achieved. According to experiments, the PLGA cytoskeleton has a great repair effect on cartilage defect.

The invention relates to the technical field of tissue engineering articular cartilage repair, and specifically relates to a double cross-linked integrated seamless composite hydrogel scaffold for articular cartilage repair. The hydrogel scaffold successively includes a subchondral bone layer, a calcified interface layer and a cartilaginous layer from bottom to top; the subchondral bone layer is the hydrogel including bioceramic particles and alginate; the calcified interface layer is the polyelectrolyte electrostatic complex hydrogel including the alginate and polycation; the cartilaginous layer is the hydrogel including the alginate; and the alginate hydrogel in the subchondral bone layer, the calcified interface layer and the cartilaginous layer firstly forms ionic crosslinked hydrogeland then forms a double cross-linked integrated seamless structure through covalent crosslinking. The mechanical strength of the hydrogel scaffold is significantly superior to ionic crosslinked hydrogel scaffolds, so that interface bonding strength can be increased; and through the polyelectrolyte composite hydrogel membrane layer in the middle of the hydrogel scaffold, isolation effects on cartilage and subchondral bone microenvironment can be realized.

The invention relates to an articular cartilage repair stent and a preparation method and application thereof. The articular cartilage repair stent comprises, from bottom to top: cancellous bone; a first cortical bone; and a first platelet-rich plasma gel layer, the first platelet-rich plasma gel layer is bonded to an upper surface of the first cortical bone, and the cancellous bone and the firstcortical bone are incompletely decalcified bone. When the articular cartilage repair stent is matched with a microfracture to repair articular cartilage defects, the microfracture provides seed cells,the cancellous bone and the first cortical bone are used as stent materials, the first platelet-rich plasma gel provides growth factors, the resulting new cartilage is hyaline cartilage rather than fibrous cartilage, the new hyaline cartilage is large in number and high in elastic modulus and hardness, and the repair area is complete and has good flatness.

The invention discloses a porous semi-degraded hydrogel material used for cartilage repair and a preparation method thereof, belonging to the technical field of articular cartilage repair. The preparation method is specifically characterized by adding mixed liquor of degradable PLGA (poly(lactic-co-glycolic acid)) microspheres with biological activities and a compound pore-foaming agent to a hydrogel solution and finally obtaining the porous semi-degraded hydrogel material through several times of physical crosslinking of freezing and unfreezing after stirring the solution uniformly at high temperature. The hydrogel material has the obvious advantages that the advantage of biological activities of the PLGA microspheres and the advantage of excellent biomechanical properties of the hydrogel material are combined; the porous structure of the prepared porous hydrogel material containing the PLGA microspheres is suitable for cell seeding and migration; regeneration of cartilage tissues is induced by degradation of the PLGA microspheres, so that the problem of connection between repair materials and surrounding tissues can be effectively solved; and meanwhile, the excellent mechanical properties of the hydrogel material are conductive to ensuring the mechanical strength of implanting initial materials.

The invention relates to an articular cartilage repairing material based on oxidized hyaluronic acid-type II collagen and autologous concentration bone marrow nucleated cells and a preparation method.The articular cartilage repairing material is prepared from oxidized hyaluronic acid, type II collagen and autologous concentration bone marrow nucleated cells; the autologous concentration bone marrow nucleated cells in the material have potential in self-renewal and chondrogenic differentiation, participate in cartilage defect repairing, and thus realize a perfect cartilage defect repairing effect; the repaired tissue appears like hyaline cartilage and is obviously superior to the blank control group in surface flatness, integration degree with adjacent normal cartilage, content of type IIcollagen, GAG content, forms of calcified cartilage and subchondral bone and the like, and has potential in clinical transformation.

Disclosed is a health product for diminishing inflammation, reducing pain and facilitating renovation of articular cartilage, which comprises the constituents of tiocondramina 800-1500mg, chondroitin sulfate 600-1000mg, fresh ginger extract 400-600mg, and green tea extract 200-400mg. The medicament can be made into tablets by mixing the raw materials homogeneously.

The invention relates to the technical field of tissue engineering articular cartilage repair, and in particular relates to a composite scaffold for hip articular cartilage repair and a preparation method of the composite scaffold. The scaffold is provided with a porous magnesium scaffold layer wrapped by bioceramics and a polymer hydrogel layer from the bottom to the top in sequence, the hydrogellayer is loaded with chondrocytes, and a three-layer structure including a subchondral bone layer and a calcification interface layer and a cartilage layer is formed after chondrocytes are implantedin a body. After the composite scaffold provided by the invention encounters a body fluid in the microenvironment of in-vivo tissue, the porous magnesium component in the scaffold is oxidized to generate hydrogen, the generated gas cannot be immediately diffused due to the limitation of a surface ceramic membrane, a gas film is formed locally, a stable isolation layer is formed, and an osteogenicmicroenvironment is isolated from a cartilage microenvironment, so that cells and cytokines in the osteogenic microenvironment below the cartilage microenvironment are prevented from entering the cartilage microenvironment, cartilage ossification can be prevented, the stability of the cartilage microenvironment is effectively maintained, and the composite scaffold is suitable for large-area cartilage repair, wherein the diameter of the repaired area is greater than 1 cm.

The invention provides a preparation method and application of a cell-stent composite material for cartilage injury repairing, and belongs to the fields of bone tissue engineering and regenerative medicines. Reprogrammed cells with multi-directional differentiation potential and relatively good security are adopted as seed cells for preparing the cell-stent composite material, a biological material which is prepared from konjac glucomannan and sodium hyaluronate through mixing is adopted as a stent, and the cell-stent composite material is prepared from the cells and the stent by using a co-culture method. The cell-stent composite material is relatively simple in preparation method, is capable of effectively repairing cartilage injury, and has wide application prospects in the fields of bone tissue engineering and regenerative medicines, particularly in the field of joint cartilage repairing.