Double cross-linked integrated seamless composite hydrogel scaffold for articular cartilage repair

Abstract

The invention relates to the technical field of tissue engineering articular cartilage repair, and specifically relates to a double cross-linked integrated seamless composite hydrogel scaffold for articular cartilage repair. The hydrogel scaffold successively includes a subchondral bone layer, a calcified interface layer and a cartilaginous layer from bottom to top; the subchondral bone layer is the hydrogel including bioceramic particles and alginate; the calcified interface layer is the polyelectrolyte electrostatic complex hydrogel including the alginate and polycation; the cartilaginous layer is the hydrogel including the alginate; and the alginate hydrogel in the subchondral bone layer, the calcified interface layer and the cartilaginous layer firstly forms ionic crosslinked hydrogeland then forms a double cross-linked integrated seamless structure through covalent crosslinking. The mechanical strength of the hydrogel scaffold is significantly superior to ionic crosslinked hydrogel scaffolds, so that interface bonding strength can be increased; and through the polyelectrolyte composite hydrogel membrane layer in the middle of the hydrogel scaffold, isolation effects on cartilage and subchondral bone microenvironment can be realized.

Description

technical field [0001] The invention relates to the technical field of tissue engineering articular cartilage repair, in particular to a subchondral bone-cartilage integrated double-crosslinked composite hydrogel scaffold used for articular cartilage repair throughout the whole process and a preparation method thereof. Background technique [0002] Articular cartilage injury is a common clinical disease. Articular cartilage lacks blood vessels, nerves, and lymphatic tissues, and contains very few cells, so its self-repair ability is limited. Therefore, it is difficult to restore articular cartilage damage to a healthy state no matter whether it is caused by physical or chemical factors. At present, the commonly used clinical methods for the treatment of cartilage injuries, such as minimally invasive drilling, microfracture, mosaic transplantation, periosteum or perichondrium transplantation, etc., although they can alleviate the pain of patients to a certain extent, the long...

AI Technical Summary

Problems solved by technology

However, the above-mentioned electrostatic complexation technology, ion cross-linking technology, and 3D printing technology still have poor interface bonding force, and the osteochondral interface delamination phenomenon is prone to occur under the action of joint movement shear force

However, the iterative freeze-drying technology and ultraviolet cross-linking technology cannot realize the preparation of integrated interface seamless scaffolds loaded with cells because the preparation conditions are not mild during the process, resulting in cell death.

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