59 results about "Cross linked hydrogels" patented technology

The invention relates to the technical field of biomedical material modification, in particular to a sulfhydrylation biocompatible polymer derivative, a preparation method thereof, extracellular matrix imitating injectable in-situ cross-linked hydrogel and application thereof. According to the invention, a pre-acylation strategy is firstly carried out on the biocompatible polymer containing both amino and carboxyl, so that a three-dimensional cross-linked byproduct formed by coupling of self amido bonds is avoided, meanwhile, the number of side chain carboxyl for sulfhydrylation modification is increased, and the accurate and adjustable pre-acylation rate can optimize the physical and chemical properties of the derivative; meanwhile, a disulfide bond is used as a protective precursor, active sulfydryl is introduced through amido bond coupling, self-oxidation of the active sulfydryl in the reaction process is avoided, sulfydryl activity is guaranteed, hydrazine-containing reagents are avoided, safety is further improved, virus residues and immunological rejection risks are avoided, universality is high, and the application prospect is wide; and in combination with a high-concentration preparation method, the method has the advantage of large-scale production, and further widens the industrial application potential.

The invention discloses a photosensitizer, dynamic antibacterial hydrogel containing lipidosome wrapping the photosensitizer and a preparation method and application of the dynamic antibacterial hydrogel, and belongs to the technical field of biological materials. The invention provides a novel D-A-D type photosensitizer TPT which is simple in structure and simple in synthetic route, and from the angles of safety, practicability and use specificity of hydrogel dressing, modified hyaluronic acid and modified carboxymethyl chitosan are used as raw materials, cationic liposome wrapping the novel photosensitizer TPT is carried, and the hydrogel dressing is prepared. The efficient antibacterial dynamic cross-linked hydrogel material is prepared. The hydrogel disclosed by the invention has two pH response bonds, namely an imine bond and a boric acid ester bond, and has good moisture retention and biocompatibility; meanwhile, the hydrogel is loaded with the cationic liposome wrapping the novel photosensitizer TPT, so that the hydrogel has excellent active oxygen yield. The hydrogel disclosed by the invention aims to quickly resist bacteria, promote wound healing, reduce infection risk and improve the success rate of treatment through the targeted drug release function of the intelligent hydrogel.

The invention discloses a core amino acid sequence for driving any recombinant protein to be self-assembled into supramolecular hydrogel and application of the core amino acid sequence, and aims to solve the problems of complex synthesis, toxic residue, insufficient biocompatibility, difficulty in performance regulation and control and the like of the traditional chemical crosslinking hydrogel. The whole length of Ure2 (1-19) to Ure2 (1-71) and any truncated body and mutant are covered, and the shortest sequence Ure2 (1-19) has efficient driving self-assembly. The core amino acid sequence is fused to any recombinant protein, the obtained fusion protein can form nanofiber-shaped self-assembly aggregation when the concentration is lower than 1 mg / mL, and can form supramolecular hydrogel with the characteristics of shear thinning effect, injectability and the like when the concentration is greater than or equal to 1 mg / mL, and the formation of the hydrogel does not need any additional artificial interference. The preparation can be finished only by standing for a period of time.

This invention relates to a composition for promoting hair growth, comprising a dynamically cross-linked hydrogel loaded with dermal papilla cells. The dynamically cross-linked hydrogel includes adamantane-grafted hyaluronic acid, gelatin, and cyclodextrin modified with cross-linking groups. A three-dimensional network of the dynamic hydrogel is formed through host-guest interactions and the cross-linking of the cross-linking groups. The invention also provides a method for preparing the composition for promoting hair growth, comprising mixing adamantane-grafted hyaluronic acid and cyclodextrin modified with cross-linking groups to obtain a hydrogel prepolymer; adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a cross-linking reaction of the cross-linking groups to form a dynamically cross-linked hydrogel loaded with dermal papilla cells. The composition can be directly delivered to the depth of hair follicles in the scalp via mesotherapy injection to activate dormant hair follicles from the root, promote new hair growth, and thicken existing hair. The hair regeneration effect is rapid and long-lasting.

The invention relates to a composition comprising a cell-free lipoaspirate-derived preparation comprising a lipid fraction and / or an aqueous fraction of a lipoaspirate and at least one biodegradable crosslinked hydrogel. The invention further relates to a device and a kit comprising the composition. The composition, the device and the kit can be generally applied for aesthetic treatment of soft-tissue loss or deficit.

The present application relates to a kind of polyamide aerogel film with coordination-covalent double crosslinking network structure and its preparation method and application, with carboxyl-containing diamine, carboxyl-free diamine, diacyl chloride and blocking agent as raw material, solution polymerization is carried out in the presence of organic base, and the polyamide oligomer solution containing polymerizable double bond in end group is obtained;The purified and dried polyamide oligomer is dissolved in aqueous solution containing organic base, and metal salt is added to form coordination crosslinking hydrogel film, and double crosslinking network is constructed by initiating end group double bond polymerization by ultraviolet irradiation, and finally freeze-dried to obtain polyamide aerogel film.The polyamide aerogel film of the present application has high porosity, excellent flexibility and mechanical stability, can efficiently and selectively adsorb heavy metal ions in water body, and can be regenerated efficiently and recycled multiple times by mild acid desorption-alkali activation combined regeneration process, aiming at providing a kind of high-performance, low-cost and sustainable solution for water treatment and environmental remediation field.

The application belongs to the technical field of medical polymer materials, and particularly relates to an alkoxy silane PEG crosslinking agent, a physical / chemical double crosslinking hydrogel and application thereof. The application utilizes a small molecule alkoxy silane which is insoluble in water to modify the end of a PEG macromolecule, and after forming a homogeneous system with a physical hydrogel, the alkoxy silane is slowly hydrolyzed and crosslinked, and then a physical / chemical double crosslinking hydrogel is formed. Moreover, the physical / chemical double crosslinking hydrogel disclosed in the application combines the advantages of a physical hydrogel and a chemical hydrogel: the water system before crosslinking has good temperature sensitivity and shear thinning characteristics, and after mixing various functional additives at low temperature, the water system can be delivered through a medical needle tube and a catheter; the physical crosslinking thermally induced gel is in situ gelled at an application site and is beneficial to subsequent chemical crosslinking; after being injected into a target site, the chemical crosslinking reaction gradually occurs, and after complete crosslinking, the mechanical properties and stability of the hydrogel are greatly improved.

In some aspects, the present disclosure pertains to systems for forming hydrogels that comprise: (a) a reactive multi-functional compound comprising (i) a residue of a polyol that comprises two or more hydroxyl groups and (ii) a plurality of activated ester groups, each of which is linked to the polyol residue through a residue of a cyclic anhydride and (b) a reactive polymer comprising a plurality of nucleophilic groups that are reactive with the plurality of activated ester groups of the reactive multi-functional compound. In other aspects, the present disclosure provides methods of treatment comprising administering to a subject a mixture of the reactive multi-functional compound and the multi-arm polymer to a patient. In other aspects, the present disclosure provides crosslinked hydrogels that are formed from the reactive multi-functional compound and the multi-arm polymer.

The invention discloses a composite hydrogel grouting material and a preparation method and application thereof, and belongs to the technical field of hydrogel grouting materials, the composite hydrogel grouting material is a two-component system, and the two-component system comprises a component A and a component B; the component A and the component B are mixed to form an ionic covalent bi-crosslinking system, that is, calcium lactate / calcium gluconate in the component B and sodium alginate in the component A are rapidly subjected to ionic crosslinking, initial setting within 15-38 seconds is achieved, and it is ensured that slurry is rapidly formed after being injected into a flowing water channel and cannot be washed away by water flow; meanwhile, acrylamide and N, N '-methylene bisacrylamide form covalent crosslinking under the action of an initiator-accelerant, so that the structural stability of the gel is improved. The contradiction that the existing single cross-linked hydrogel is fast in gelation and low in strength and high in strength and slow in gelation is broken, and efficient leaking stoppage can be realized in a flowing water environment with the flow velocity of 0.1-1.0 m / s.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

The invention discloses an injectable photo-crosslinking hydrogel loaded with pseudolaric acid as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) respectively dissolving Gelma and HAMA in sterile PBS (Phosphate Buffer Solution) according to the dosage required by a final system; 2) mixing the two solutions, and adding a photoinitiator LAP pre-dissolved in the sterile PBS at 60 DEG C; 3) dissolving golden larch bark acetic acid in dimethyl sulfoxide to prepare a medicine solution with the concentration of 1.0 mg / mL, and then adding the medicine solution into the precursor solution under the condition of continuous stirring; and 4) carrying out 405 nm illumination crosslinking on the obtained precursor solution to form the hydrogel. The hydrogel three-dimensional network can continuously and controllably release pseudolaric acid, maintains a long-acting effective drug concentration at an infected part, shows a strong inhibition effect on a candida albicans standard strain and a clinical drug-resistant strain, and has a good application prospect in a model for treating candida vaginitis and fungal skin wound infection. And all the components show a remarkable'anti-infection-repair promoting 'integrated treatment effect.

The present invention relates to a method for dynamic filtration of a cross-linked biopolymer-based hydrogel to remove unwanted molecules from the gel. In particular, the invention relates to dynamic filtration of a hyaluronic acid hydrogel using a dynamic filtration construction with rotating and semipermeable filter discs.

In some aspects, the present disclosure pertains to systems that comprise (a) a reactive polymer comprising a plurality of first hydrophilic polymer segments and a plurality of first reactive moieties, (b) a reactive multifunctional compound comprising a plurality of second reactive moieties, and (c) gold nanoparticles that are functionalized with a plurality of second hydrophilic polymer segments. In other aspects, the present disclosure pertains to crosslinked reaction products formed from such systems. In further aspects, the present disclosure pertains to methods of treatment using systems and such crosslinked reaction products.

A hydrogel-based biological delivery vehicle used to effectively deliver drug and biological material to tissue or organ sites. More specifically, a hydrogel binding matrix having a biopolymer backbone containing carboxyl groups. Tyramine may be substituted for at least a portion of the carboxyl groups, so that, when hydrogen peroxide is added, it causes creation of covalent bonds between tyramine molecules and cross-links the hydrogel binding matrix, thereby enabling the hydrogel binding matrix to transition from liquid to gel state. The hydrogel binding matrix, in its liquid form, is capable of encapsulating drug reservoirs to create a homogenous liquid with evenly distributed particles containing drugs or target molecules. As the hydrogel binding matrix solidifies into a gel state, the newly created cross-links do not disrupt or react with the drugs or target molecules contained within the drug reservoirs. This hydrogel-based biological delivery vehicle can be used in several medical applications.

The invention discloses a low-energy photocuring ophthalmic sealant and a preparation method and application thereof.The ophthalmic sealant is in-situ photocuring bi-crosslinking biological hydrogel, a functional complementary composite system is constructed through collaborative optimization, and the ophthalmic sealant mainly comprises methacrylated gelatin (GelMA), a cornea extracellular matrix component, glutamine transaminase and a photoinitiator. According to the invention, a double-network construction strategy combining low-energy photocuring and enzymatic crosslinking is adopted, and curing can be triggered under the extremely low optical power density not higher than 5 mW / cm, so that the clinical application bottlenecks of tissue damage caused by too high energy of a traditional photocuring material for eyes and insufficient mechanical property and stability of a single crosslinking system are overcome. The sealing agent has excellent tissue adhesion, mechanical stability and biocompatibility in a double-crosslinking hydrogel state, can realize minimally invasive sutureless repair of corneal defects, and effectively promotes functional regeneration of corneal tissues.

The invention discloses a preparation method of an AHP-8 and jellyfish collagen co-crosslinked hydrogel microneedle patch. The preparation method comprises the following steps: (1) preparing jellyfish collagen; (2) preparing modified jellyfish collagen; (3) preparing a mixed solution of modified jellyfish collagen and poly (N-isopropylacrylamide); (4) preparing an AHP-8 poly (N-isopropylacrylamide) mixed solution; (5) preparing the microneedle patch in the microneedle patch silica gel mold; and (6) irradiating by using an ultraviolet lamp for crosslinking, and demolding to obtain the AHP-8 and jellyfish collagen co-crosslinked hydrogel multi-layer microneedle patch. The microneedle patch disclosed by the invention has the advantages of low sensitization, high-efficiency aging resistance and intelligent release transdermal drug delivery.

The invention relates to the technical field of biological medicine, and discloses dual-network hydrogel for treating inflammatory bowel disease as well as a preparation method and application of the dual-network hydrogel. The preparation method of the double-network hydrogel comprises the following steps: dissolving glycyrrhizic acid into deionized water, and then adding beta-lactoglobulin to obtain a solution A; dissolving zinc chloride and 2, 3, 4-trihydroxybenzoic acid in deionized water to obtain a solution B; mixing the solution A and the solution B in proportion, and stirring for reaction to obtain the double-network hydrogel. A main framework of the double-network hydrogel is composed of a first-stage network formed by glycyrrhizic acid and zinc ions through self-crosslinking and a second-stage network formed by beta-lactoglobulin and 2, 3, 4-trihydroxybenzoic acid through Schiff base and hydrogen bond crosslinking. The invention provides the dual-crosslinked hydrogel with the skeleton being a therapeutic effective component and good plasticity and adhesion, and the dual-crosslinked hydrogel has the advantages of resistance to external severe environment and strong anti-inflammatory ability, and can effectively relieve mouse colitis.

The invention relates to a photoresponsive self-initiated cross-linked hydrogel, and raw materials, a preparation method and application thereof. The method comprises the following steps: carrying out amidation reaction on a carboxyl-containing natural polymer and aminobenzophenone to obtain the photoresponsive self-initiated cross-linked hydrogel; or the amino-containing natural polymer and carboxyl benzophenone are subjected to an amidation reaction to obtain the amino-containing natural polymer. The photoresponsive self-initiation cross-linked polymer can be used as a macromolecular photoinitiator of a photosensitive polymer, and an aqueous solution of a single polymer of the photoresponsive self-initiation cross-linked polymer can be cross-linked under the irradiation of light with specific wavelength to form hydrogel, so that the photoinitiator can be effectively prevented from generating toxicity due to permeation.

The application belongs to the technical field of biological medicine, and particularly relates to a double-network hybrid cross-linked hydrogel, a preparation method and application in 3D organoid culture. The double-network hybrid cross-linked hydrogel is formed by natural cross-linking of a hydrogel precursor material at room temperature, and the double-network hybrid cross-linking includes covalent cross-linking and hydrogen bond cross-linking formed between the hydrogel precursor materials; the hydrogel precursor material includes mercapto-hyaluronic acid-hexanediamine-cytosine, mercapto-hyaluronic acid-hexanediamine-guanine and a polyethylene glycol compound with a multi-arm star structure. The hydrogel has both the mechanical stability of covalent cross-linking and the dynamic responsiveness of hydrogen bond cross-linking, and can be quickly formed under physiological conditions, thereby providing a new matrix material for organoid culture, which is safe and stable, has clear components and can dynamically simulate a tumor microenvironment.

The invention relates to a melatonin-loaded bi-crosslinking hydrogel and an application thereof. According to the invention, polylysine (PL-GA) grafted with gallic acid is used as an intelligent carrier, hyaluronic acid (HA-PBA-GMA) grafted with phenylboronic acid and glycidyl methacrylate is used as a responsive network skeleton and a stable skeleton precursor, and melatonin is loaded on the PL-GA to form a drug-loading functional unit (MT at PL-GA); mT < PL-GA > and an HA-PBA-GMA solution are mixed, and the bi-crosslinked hydrogel with environmental responsiveness and structural stability is prepared through'dynamic boric acid ester bond crosslinking 'and'photo-initiated free radical polymerization'. The melatonin loading rate of the double-crosslinking hydrogel is high, the double-crosslinking hydrogel has excellent stability and self-healing performance, meanwhile, the mechanical property of the double-crosslinking hydrogel is matched with the mechanical environment of a wound surface, and the double-crosslinking hydrogel can release drugs in a responsive mode according to the wound microenvironment, so that the double-crosslinking hydrogel is very suitable for treatment of local wounds.

The invention provides a cross-linked hydrogel microneedle for transdermal drug delivery and a preparation method of the cross-linked hydrogel microneedle. Mixing gelatin and cross-linking group modified cyclodextrin to form a hydrogel prepolymer, mixing the hydrogel prepolymer with cells, and initiating a cross-linking reaction in the presence of a chemical cross-linking initiator or a photoinitiator under the illumination condition to obtain cell-loaded cross-linked hydrogel; the preparation method comprises the following steps: respectively dissolving carboxymethyl chitosan, MTGase enzyme and silk fibroin peptide powder in a PBS (Phosphate Buffer Solution) to obtain three initial solutions; placing the methacrylated chitosan solution in a centrifugal tube, adding a photoinitiator, and carrying out vortex and ultrasonic treatment until the photoinitiator is completely dissolved to obtain a filling solution; compared with the prior art, the hydrogel microneedle has the following beneficial effects that the hydrogel microneedle is prepared by pouring the hydrogel into a mold and then demolding, the medicine carrying capacity of the hydrogel microneedle prepared by the invention is as high as 36.06%, the medicine carrying efficiency is as high as 73.26%, the medicine carrying capacity is high, the medicine components are dissolved and released after the microneedle is in contact with tissue fluid, and the treatment purpose is achieved.

The invention discloses dynamic hydrogel with an effect of inhibiting melanogenesis and a preparation method of the dynamic hydrogel. The hydrogel is based on a dynamic covalent cross-linked network of carboxymethyl chitosan (CMCS) and aldehyde sodium alginate (ASA), and is compounded with functional ultraviolet shielding nano TiO2 particles, so that the hydrogel has excellent ultraviolet barrier property. By optimizing the nano TiO2 particles, the activity of tyrosinase can be inhibited while efficient blocking of ultraviolet rays is realized, and melanin generation is reduced. The dynamic hydrogel provided by the invention has ultraviolet shielding and melanin inhibition functions, can reduce the pigmentation risk, and has more comprehensive application value in the fields of skin repair and cosmetic medicine.

The application belongs to the technical field of hydrogel and provides a dynamic cross-linking hydrogel and a preparation method and application thereof, and comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing a phenylboronic acid modified group; adding dextranase before hydrogel formation, and introducing polydopamine-coated calcium peroxide nanoparticles; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the dextranase concentration is 10-100 U, and the concentration of the polydopamine-coated calcium peroxide nanoparticles is 0.1-1% w / v. The hydrogel of the application shows good cell compatibility, pro-angiogenic capacity and biological activity for promoting dental pulp regeneration in a plurality of in-vitro and in-vivo experiments, is suitable for the field of tissue engineering such as dental pulp injury repair, and effectively solves technical problems such as insufficient cell survival support of conventional hydrogels in three-dimensional culture.

The invention discloses a preparation method of a photo-crosslinking hydrogel microneedle loaded with a finasteride drug, which comprises the following three steps: preparing drug nanoparticles, synthesizing a hydrogel precursor and preparing the photo-crosslinking hydrogel microneedle. The preparation method specifically comprises the steps of preparing finasteride (FIN)-loaded polyhydroxyalkanoate (PHA) nanoparticles (F-PHANPs), preparing F-PHANPs-loaded HAMA hydrogel (F-HAMA) and preparing a hydrogel microneedle, introduction of the F-PHANPs enhances the mechanical performance of the hydrogel, meanwhile, the hardness is remarkably enhanced, and the hydrogel has excellent drug release performance and biocompatibility and can be used for preparing the hydrogel microneedle. Potential application prospects are shown in the field of treating androgenetic alopecia through transdermal drug delivery.

The invention discloses a preparation method and application of injectable photo-crosslinked GelMA / MPDA hydrogel, and belongs to the technical field of medical biological material preparation. The method comprises the following steps: preparing methacrylic anhydride gelatin (GelMA) and polydopamine beads (MPDA) with a mesoporous structure; the preparation method comprises the following steps: mixing a GelMA aqueous solution, MPDA and a photoinitiator, and stirring in a dark place to prepare the injectable photo-crosslinked GelMA / MPDA hydrogel. After being cured, the injectable hydrogel has a uniform honeycomb porous structure and has good injectability and biocompatibility, the photothermal conversion performance of the material is remarkably improved, tumor thermal therapy triggered by near-infrared light is achieved, drug loading and light-controlled release are achieved through mesoporous adsorption and chemical action, and the injectable hydrogel has a good application prospect. Therefore, a novel active controllable drug delivery platform is provided for photothermal-chemical synergistic treatment of tumors.

According to the hydrogel material disclosed by the invention, the gelatin / gallic acid conjugate with a specific concentration is taken as a base material, and the base material is cross-linked by utilizing a horse radish peroxidase / hydrogen peroxide combined enzyme catalysis system, so that an additional photo-thermal response material does not need to be added in the gelling process of the hydrogel; in the cross-linking process, the hydrogel serves as a cross-linked structure for forming the interior of the hydrogel and shows excellent photo-thermal responsiveness, and the hydrogel can respond to photo-thermal temperature rise to improve the self deformability, so that irregular wounds are better covered; therefore, the invention provides a hydrogel material which has good biological safety, oxidation resistance and bacteriostasis and can be used for wound injury repair.

The invention relates to a composition comprising a cell-free lipoaspirate-derived preparation comprising a lipid fraction and / or an aqueous fraction of a lipoaspirate and at least one biodegradable crosslinked hydrogel. The invention further relates to a device and a kit comprising the composition. The composition, the device and the kit can be generally applied for aesthetic treatment of soft-tissue loss or deficit.