18 results about "Cross linked hydrogels" patented technology

This invention relates to a composition for promoting hair growth, comprising a dynamically cross-linked hydrogel loaded with dermal papilla cells. The dynamically cross-linked hydrogel includes adamantane-grafted hyaluronic acid, gelatin, and cyclodextrin modified with cross-linking groups. A three-dimensional network of the dynamic hydrogel is formed through host-guest interactions and the cross-linking of the cross-linking groups. The invention also provides a method for preparing the composition for promoting hair growth, comprising mixing adamantane-grafted hyaluronic acid and cyclodextrin modified with cross-linking groups to obtain a hydrogel prepolymer; adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a cross-linking reaction of the cross-linking groups to form a dynamically cross-linked hydrogel loaded with dermal papilla cells. The composition can be directly delivered to the depth of hair follicles in the scalp via mesotherapy injection to activate dormant hair follicles from the root, promote new hair growth, and thicken existing hair. The hair regeneration effect is rapid and long-lasting.

The invention relates to a composition comprising a cell-free lipoaspirate-derived preparation comprising a lipid fraction and / or an aqueous fraction of a lipoaspirate and at least one biodegradable crosslinked hydrogel. The invention further relates to a device and a kit comprising the composition. The composition, the device and the kit can be generally applied for aesthetic treatment of soft-tissue loss or deficit.

The present application relates to a kind of polyamide aerogel film with coordination-covalent double crosslinking network structure and its preparation method and application, with carboxyl-containing diamine, carboxyl-free diamine, diacyl chloride and blocking agent as raw material, solution polymerization is carried out in the presence of organic base, and the polyamide oligomer solution containing polymerizable double bond in end group is obtained;The purified and dried polyamide oligomer is dissolved in aqueous solution containing organic base, and metal salt is added to form coordination crosslinking hydrogel film, and double crosslinking network is constructed by initiating end group double bond polymerization by ultraviolet irradiation, and finally freeze-dried to obtain polyamide aerogel film.The polyamide aerogel film of the present application has high porosity, excellent flexibility and mechanical stability, can efficiently and selectively adsorb heavy metal ions in water body, and can be regenerated efficiently and recycled multiple times by mild acid desorption-alkali activation combined regeneration process, aiming at providing a kind of high-performance, low-cost and sustainable solution for water treatment and environmental remediation field.

The application belongs to the technical field of medical polymer materials, and particularly relates to an alkoxy silane PEG crosslinking agent, a physical / chemical double crosslinking hydrogel and application thereof. The application utilizes a small molecule alkoxy silane which is insoluble in water to modify the end of a PEG macromolecule, and after forming a homogeneous system with a physical hydrogel, the alkoxy silane is slowly hydrolyzed and crosslinked, and then a physical / chemical double crosslinking hydrogel is formed. Moreover, the physical / chemical double crosslinking hydrogel disclosed in the application combines the advantages of a physical hydrogel and a chemical hydrogel: the water system before crosslinking has good temperature sensitivity and shear thinning characteristics, and after mixing various functional additives at low temperature, the water system can be delivered through a medical needle tube and a catheter; the physical crosslinking thermally induced gel is in situ gelled at an application site and is beneficial to subsequent chemical crosslinking; after being injected into a target site, the chemical crosslinking reaction gradually occurs, and after complete crosslinking, the mechanical properties and stability of the hydrogel are greatly improved.

In some aspects, the present disclosure pertains to systems for forming hydrogels that comprise: (a) a reactive multi-functional compound comprising (i) a residue of a polyol that comprises two or more hydroxyl groups and (ii) a plurality of activated ester groups, each of which is linked to the polyol residue through a residue of a cyclic anhydride and (b) a reactive polymer comprising a plurality of nucleophilic groups that are reactive with the plurality of activated ester groups of the reactive multi-functional compound. In other aspects, the present disclosure provides methods of treatment comprising administering to a subject a mixture of the reactive multi-functional compound and the multi-arm polymer to a patient. In other aspects, the present disclosure provides crosslinked hydrogels that are formed from the reactive multi-functional compound and the multi-arm polymer.

In some aspects, the present disclosure pertains to systems that comprise (a) a reactive polymer comprising a plurality of first hydrophilic polymer segments and a plurality of first reactive moieties, (b) a reactive multifunctional compound comprising a plurality of second reactive moieties, and (c) gold nanoparticles that are functionalized with a plurality of second hydrophilic polymer segments. In other aspects, the present disclosure pertains to crosslinked reaction products formed from such systems. In further aspects, the present disclosure pertains to methods of treatment using systems and such crosslinked reaction products.

The application discloses a kind of low molecular weight fucoidan loaded dynamic borate ester crosslinking antiallergic hydrogel construction method and application to resist atopic dermatitis, belong to medical biomaterial field, to solve the poor performance of existing fucoidan hydrogel carrier, drug release effect is not good.The present application extracts and purifies natural fucoidan with Sargassum fusiforme as raw material, and obtains low molecular weight fucoidan by mild degradation using hydrogen peroxide-vitamin C system;3-carboxyphenylboronic acid is grafted to gelatin by N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbonyldiimidazole hydrochloride mediated reaction, and then polyvinyl alcohol is obtained by dynamic borate ester crosslinking to obtain crosslinked hydrogel.The hydrogel obtained by the present application has good self-repairing and biocompatibility, can long-acting release active ingredients, and the hydrogel is applied to resist atopic dermatitis.

The application belongs to the technical field of biological medicine, and particularly relates to a double-network hybrid cross-linked hydrogel, a preparation method and application in 3D organoid culture. The double-network hybrid cross-linked hydrogel is formed by natural cross-linking of a hydrogel precursor material at room temperature, and the double-network hybrid cross-linking includes covalent cross-linking and hydrogen bond cross-linking formed between the hydrogel precursor materials; the hydrogel precursor material includes mercapto-hyaluronic acid-hexanediamine-cytosine, mercapto-hyaluronic acid-hexanediamine-guanine and a polyethylene glycol compound with a multi-arm star structure. The hydrogel has both the mechanical stability of covalent cross-linking and the dynamic responsiveness of hydrogen bond cross-linking, and can be quickly formed under physiological conditions, thereby providing a new matrix material for organoid culture, which is safe and stable, has clear components and can dynamically simulate a tumor microenvironment.

The invention relates to a composition comprising a cell-free lipoaspirate-derived preparation comprising a lipid fraction and / or an aqueous fraction of a lipoaspirate and at least one biodegradable crosslinked hydrogel. The invention further relates to a device and a kit comprising the composition. The composition, the device and the kit can be generally applied for aesthetic treatment of soft-tissue loss or deficit.

This invention provides a method for preparing dermal papilla cell spheres, comprising mixing hydrophobic hyaluronic acid grafted with hydrophobic groups and cyclodextrin modified with crosslinking groups to obtain a hydrogel prepolymer, adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a crosslinking reaction of the crosslinking groups to form a dynamically crosslinked hydrogel loaded with cells, and then adding a cell culture medium. The hydrophobic groups are selected from tert-butylbenzene, ibuprofen, menthol, etc. This method can rapidly obtain dermal papilla cell spheres and maintain the stemness of dermal papilla cells and the induction ability of hair follicle tissue, which can be used to achieve hair follicle tissue repair and regeneration, and promote hair growth. This invention also relates to the application of the method for preparing dermal papilla cell spheres in the preparation of compositions that promote hair follicle tissue repair, regeneration, or hair growth, wherein the composition includes a dynamically crosslinked hydrogel loaded with dermal papilla cells, which can be used to prevent and treat hair loss.

This invention relates to the field of biomedical technology, disclosing a dual-network hydrogel for treating inflammatory bowel disease, its preparation method, and its application. The preparation method of the dual-network hydrogel is as follows: glycyrrhizic acid is dissolved in deionized water, and then β-lactoglobulin is added to obtain solution A; zinc chloride and 2,3,4-trihydroxybenzoic acid are dissolved together in deionized water to obtain solution B; solutions A and B are mixed in a certain proportion and stirred to obtain the dual-network hydrogel. The main framework of the dual-network hydrogel in this invention consists of a first-level network formed by self-crosslinking of glycyrrhizic acid and zinc ions, and a second-level network formed by crosslinking of β-lactoglobulin and 2,3,4-trihydroxybenzoic acid through Schiff bases and hydrogen bonds. This invention provides a dual-crosslinked hydrogel whose own framework is the therapeutic active ingredient, possessing good plasticity and adhesion, resisting harsh external environments, exhibiting strong anti-inflammatory capabilities, and effectively alleviating colitis in mice.

This invention belongs to the field of biomaterials and discloses a protein-based hydrogel in-situ crosslinking agent. The in-situ crosslinking agent is characterized by being composed of a first precursor solution and a second precursor solution. The first precursor solution is prepared by dissolving thiolated polyethylene glycol (PEGSH) and protein in a buffer solution and adjusting the pH to 7.2 with NaOH. The second precursor solution is prepared by dissolving CuSO4 in deionized water. This protein-based hydrogel in-situ crosslinking agent possesses injectability, rapid gelation properties, and the hydrogel formed after crosslinking exhibits strong self-healing ability, low swelling ratio, good elasticity, and is not easily crushed. Furthermore, it can rapidly disintegrate under the action of a degelating agent.

In some aspects, the present disclosure provides a hydrogel comprising (a) a reactive multi-armed polymer comprising a plurality of reactive moieties and (b) a crosslinking reaction product of a multifunctional crosslinking compound having a plurality of complementary reactive moieties that react with the reactive moieties of the reactive multi-armed polymer, the hydrogel comprising hydrolysable moieties as described herein dispersed throughout the hydrogel. Other aspects of the present disclosure relate to reactive multi-armed polymers and multifunctional crosslinking compounds for use in forming such hydrogels, as well as crosslinkable systems for forming such hydrolysable hydrogels.

The application provides a pH and ROS dual-response double-crosslinking network injectable hydrogel as well as a preparation method and application thereof. The hydrogel comprises solution A and solution B. The solution A contains oxidized polysaccharides, the oxidized polysaccharides have aldehyde groups, and the oxidized polysaccharides are grafted with boronic acid groups. The solution B contains high-molecular materials with amino groups, and the high-molecular materials are grafted with o-diphenol groups. The injectable hydrogel provided by the application is based on high-molecular materials, in-situ gelation in the body after injection, and a double-crosslinking hydrogel network containing pH-sensitive Schiff base bonds and ROS-sensitive borate ester bonds, so that the hydrogel has excellent mechanical properties, responsiveness and stability. Through the synergistic effect of the double-crosslinking network, the balance between the stability of the hydrogel in a normal physiological environment and the rapid response of the hydrogel in a pathological microenvironment is achieved.

The invention belongs to the technical field of food-borne pathogenic bacterium detection, and discloses a preparation method of an electrochemical luminescence biosensor based on carbon material enhanced porphyrin hydrogel. The method comprises the following steps: synthesizing a sodium alginate-chitosan-TPPS (Thermoplastic Polystyrene) composite hydrogel; au NPs is prepared; modifying the electrode; and preparing the detection liquid. According to the invention, 5, 10, 15, 20-tetra (4-sulfophenyl) porphyrin is used as a functional material, the functional material is innovatively introduced into a sodium alginate-chitosan bi-crosslinking hydrogel system, the problems of poor stability and difficulty in modification of a water-soluble porphyrin material in sensing application are effectively overcome, and meanwhile, the conductivity and ECL signal strength of the porphyrin hydrogel are enhanced by adopting the carbon nanotubes, so that the sensing performance of the water-soluble porphyrin hydrogel is improved. Therefore, accurate detection of food-borne pathogenic bacteria represented by staphylococcus aureus is achieved, and the sensing electrode is easy and convenient to prepare, high in operability, rapid in response, high in sensitivity, high in controllability and suitable for on-site rapid detection and portable analysis requirements in the fields of food production and processing, circulation supervision, public health monitoring and the like.