172 results about "Cross linked hydrogels" patented technology

The invention relates to a dynamic-covalent hybrid cross-linked hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A-phenylboronic acid modified polymer derivative in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; dissolving a component C-double bond group modified polymer derivative in a biocompatible medium to obtain a solution C; and mixing the solution A, the solution B and the solution C with certain concentrations for a certain time, and performing illumination under a photoinitiator for a certain time to obtain the dynamic-covalent hybrid cross-linked hydrogel material. Compared with single dynamic bond crosslinking or covalent bond crosslinking, the prepared hydrogel material has machinability and mechanical stability; the hydrogel material has plasticity, shear thinning, self-repairing property and the like in the early stage of crosslinking; enough mechanical strength and long-term stability are realized in the later stage of crosslinking.

The invention relates to a protein-based phenylboronic acid ester dynamic crosslinking hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A, namely a phenylboronic acid modified protein polymer derivative, in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; and mixing the solution A and the solution B with a certain concentration for a certain time to obtain the protein-based phenylboronic acid ester dynamic crosslinking hydrogel material. The boric acid ester dynamic cross-linking bond belongs to reversible chemical cross-linking reaction, and the prepared protein-based hydrogel material has enough mechanical strength and cheap operability. The protein-based hydrogel material disclosed by the invention can provide the effects of cell adhesion, proliferation and differentiation promotion, tissue regeneration and the like, can load and slowly release beneficial components for promoting tissue repair, is an ideal tissue engineering scaffold material, and can be applied to defect regeneration and repair of soft tissues, cartilages, bones and the like.

According to some embodiments, a patch for organ and / or tissue application is provided which comprises a biocompatible polymeric material having an auxetic architecture and further matching a stiffness ratio and / or Poisson's ratio of an organ or tissue to which the patch is applied. According to some further embodiments, biocompatible patches and methods of making the same are provided comprising providing a layer of hydrogel and forming an auxetic architecture by selectively crosslinking areas of the hydrogel via exposure to light, wherein the auxetic architecture is selected to match a stiffness ratio and Poisson's ratio of an organ to which the biocompatible patch is applied.

The invention belongs to the technical field of probiotic preparations, and particularly relates to bifidobacterium longum subsp. Longum and application thereof in resisting helicobacter pylori. The bifidobacterium longum subsp. Longum provided by the invention has a good inhibition effect on helicobacter pylori. Furthermore, the bifidobacterium longum subsp. Longum is effectively embedded through enzyme cross-linking and ionic cross-linking by adopting the bifidobacterium longum subsp. Longum, so that not only can the embedding rate of the strain be improved, but also the survival rate of the strain can be remarkably improved in a digestion environment, and the efficient planting of the strain is realized; particularly, the effect of resisting helicobacter pylori infection can be obviously improved. Therefore, the invention has important practical significance on prevention and treatment of helicobacter pylori infection, and can also provide a technical basis for application of anti-helicobacter pylori functional products in the future.

The invention relates to the technical field of preparation of medical supplies, in particular to a surgical membrane and a preparation process. During specific use, firstly, a polyurethane base membrane is selected and subjected to plasma treatment; spraying a nano SiO2 dispersion liquid containing a photoinitiator on the surface of the treated polyurethane base film, and then performing ultraviolet patterning irradiation to form a micro-bulge structure to obtain a base material layer; projecting the colloidal fluid to a temperature gradient mask through a digital micromirror device, so that the colloidal fluid is subjected to regional thermal polymerization on the substrate layer to form an intelligent adhesive layer with viscosity gradient; by adopting an aerosol jet printing technology, super absorbent fibers and polyvinyl alcohol-borax dynamic crosslinking hydrogel are co-deposited to form a drainage layer, so that the technical problems that a surgical membrane prepared by a surgical membrane preparation mode in the prior art is inflexible in adhesion and poor in surface structure performance, and a preparation process is difficult to accurately control are solved.

The invention discloses a hydrogel-acellular matrix composite patch containing functionalized extracellular vesicles and a preparation method of the hydrogel-acellular matrix composite patch. The composite patch comprises a hydrogel layer and an acellular pericardium matrix material layer, and an extracellular vesicle-macrophage membrane fusion body (EV-MM) is entrapped in the cross-linked hydrogel. From two aspects of improving the targeting of EVs to the cardiac infarction part and enhancing the retention of the EVs at the cardiac infarction part, the distribution condition of the EVs at the cardiac infarction part is improved, so that the action efficiency of the EVs is improved, and the treatment effect is improved.

The invention provides a novel recombinant III type collagen and a PEG (Polyethylene Glycol) cross-linked hydrogel thereof. The invention also provides an application of the PEG cross-linked hydrogel as a tissue filling agent.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

In some aspects, the present disclosure provides systems for forming hydrogels that comprise (i) a reactive multi-arm polymer that comprises three or more polymer arms linked to a core region, each arm comprising a polyether segment and a reactive moiety comprising a cyclic imide carboxy-C1-C5-alkyl ester end group or each arm comprising a polyether segment and a reactive moiety comprising a C2-C6-isocyanoalkyl end group and (ii) a polyamino compound comprising at least two amino (-NH2) groups, wherein the reactive multi-arm polymer and the polyamino compound react to form a crosslinked hydrogel that does not contain ester groups and has long-term stability in vivo. In some aspects, the present disclosure provides methods of treatment using such systems and hydrolysis-resistant crosslinked hydrogels formed from such systems.

The invention is applicable to the technical field of hydrogel, and provides dynamic cross-linked hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing phenylboronic acid modification groups; glucanase is added before the hydrogel is formed, and calcium peroxide nanoparticles coated with polydopamine are introduced; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the concentration of the glucanase is 10-100 U, and the concentration of the calcium peroxide nanoparticles coated with the polydopamine is 0.1-1% w / v. The hydrogel disclosed by the invention shows good cell compatibility, angiogenesis promoting capability and bioactivity for promoting dental pulp regeneration in multiple in-vitro and in-vivo experiments, is suitable for tissue engineering fields such as dental pulp injury repair and the like, and effectively solves the technical problem that conventional hydrogel is insufficient in cell survival support in three-dimensional culture and the like.

The invention discloses an anti-tumor intelligent double-crosslinking hydrogel drug delivery system based on a purple phosphorus nanosheet and a preparation method and application thereof, the system comprises a hydrogel carrier, a photodynamic carrier material and a drug, and the hydrogel carrier is a drug delivery carrier formed by crosslinking polydimethyldiguanide with Fe < 3 + > and a crosslinking agent; the photodynamic carrier material is a purple phosphorus nanosheet; the medicine is a medicine with two effects of inhibiting hypoxia-inducible factor-1 alpha and lowering PD-L1 down. According to the invention, the purple phosphorus nanosheet and the polydimethyldiguanide / Fe < 3 + > bi-crosslinking hydrogel are taken as carriers to prepare the tumor microenvironment response type hydrogel drug delivery system with good pH responsiveness, injectability and self-healing property, so that an effective solution is provided for tumor precise drug delivery mediated by a new material, namely the purple phosphorus nanosheet, as a nano-drug carrier; the invention also provides an effective photodynamic therapy strategy for anti-tumor application of the intelligent double-crosslinking hydrogel and precise treatment of breast cancer.

In some aspects, the present disclosure pertains to a method of forming radiopaque crosslinked hydrogel particles comprising (a) mixing a reactive multi-arm polymer comprising a plurality of cyclic imide ester groups, a reactive multifunctional compound comprising a plurality of amino groups, and a radiocontrast agent under conditions in which a pH environment surrounding the reactive multi-arm polymer, the reactive multifunctional compound and the radiocontrast agent increases from an acidic pH to a basic pH, thereby forming a radiopaque hydrogel and (b) subjecting the hydrogel to a particle size reduction process, thereby forming the radiopaque crosslinked hydrogel particles. Other aspects of the present disclosure pertain to radiopaque crosslinked hydrogel particles formed from such methods, suspensions of such radiopaque crosslinked hydrogel particles, and medical supplies that contain such radiopaque crosslinked hydrogel particles.

The application discloses a mussel-inspired multifunctional double-network crosslinked hydrogel wound dressing. A hydrogel network formed by dynamic covalent crosslinking between carboxymethyl chitosan and oxidized sodium alginate is utilized; further, a natural antioxidant, gallic acid, with a catechol structure is introduced to enhance the tightness of the hydrogel network combination and endow the hydrogel with certain adhesion and antioxidant properties; finally, a mussel adhesive fluid containing catechol and iron is imitated to have strong tissue adhesion behavior under alkaline conditions, which is suitable for dynamic wound treatment, so as to prepare a mussel-inspired multifunctional double-network crosslinked hydrogel wound dressing. In addition, catechol-Fe 3+ Metal coordination also shows strong antibacterial effect, and under the irradiation of near-infrared light, light energy can be converted into heat energy for preventing and treating wound infection, shortening wound closure time and preventing scar formation.

In some aspects, the present disclosure pertains to systems for forming crosslinked reaction products that comprises (a) a first composition that comprises a first multifunctional molecule that comprises a plurality of first reactive moieties that comprise azide groups and (b) a second composition that comprises a second multifunctional molecule that comprises a plurality of second reactive moieties that comprise cyclic alkyne groups, where at least one of the first and second multifunctional molecules is a reactive multi-arm polymer and where at least one of the first and second multifunctional molecules comprises an iodinated core. Other aspects of the present disclosure include crosslinked networks formed from such first and second multifunctional molecules and methods of treatment based on crosslinked networks formed from such first and second multifunctional molecules.

The invention relates to the technical field of fire-fighting materials, in particular to a fire-retardant thermosensitive self-crosslinking hydrogel fire extinguishing agent and a preparation method thereof. In order to solve the technical problems that an existing hydrogel fire extinguishing agent is limited in fire retardance effect, and especially for a high-temperature fire source, the existing hydrogel fire extinguishing agent is difficult to achieve a good fire retardance effect, the invention discloses a fire retardance type thermosensitive self-crosslinking hydrogel fire extinguishing agent which comprises the following components in percentage by mass: 0.05-1% of aryl sulfonylated carboxymethyl cellulose, 0.05-1% of a cross-linking agent, 0.05-1% of a cross-linking agent and the balance of water. 0.1%-2% of a first thickening agent; 0.1%-2% of a second thickening agent; 0.3 to 3 percent of a surfactant; 0.5-5% of a first flame retardant; 0.5 to 5% of a second flame retardant; and the other component is a solvent. The fire-retardant thermosensitive self-crosslinking hydrogel fire extinguishing agent can be used for rapid extinguishing and after-combustion inhibition of a high-temperature (less than or equal to 700 DEG C) fire source. And a good fire retardant effect is achieved.

The invention relates to the technical field of biomedical material modification, in particular to a sulfhydrylation biocompatible polymer derivative, a preparation method thereof, extracellular matrix imitating injectable in-situ cross-linked hydrogel and application thereof. According to the invention, a pre-acylation strategy is firstly carried out on the biocompatible polymer containing both amino and carboxyl, so that a three-dimensional cross-linked byproduct formed by coupling of self amido bonds is avoided, meanwhile, the number of side chain carboxyl for sulfhydrylation modification is increased, and the accurate and adjustable pre-acylation rate can optimize the physical and chemical properties of the derivative; meanwhile, a disulfide bond is used as a protective precursor, active sulfydryl is introduced through amido bond coupling, self-oxidation of the active sulfydryl in the reaction process is avoided, sulfydryl activity is guaranteed, hydrazine-containing reagents are avoided, safety is further improved, virus residues and immunological rejection risks are avoided, universality is high, and the application prospect is wide; and in combination with a high-concentration preparation method, the method has the advantage of large-scale production, and further widens the industrial application potential.

There is provided a method of producing a hydrogel. A first polymer functionalized with a homocysteine thiolactone of formula I is reacted to open the ring and obtain a pendant thiol group, wherein any three of the R2 are H, and the remaining R2 is selected from H, C1-C4 branched or linear alkyl, methoxy, ethoxy, and halogen;. (I) The pendant thiol group of the first polymer are then crosslinked with a pendant vinyl group on a second polymer in the presence of a gel former to obtain the hydrogel.

The invention relates to a self-healing bionic intervertebral disc capable of improving service stability and a nucleus pulposus preparation method of the self-healing bionic intervertebral disc, and belongs to the field of bionic implant prostheses. The self-healing bionic intervertebral disc capable of improving the service stability is composed of an upper end plate, a bionic fibrous ring layer, a lower end plate and a bionic nucleus pulposus, the bionic nucleus pulposus adopts dynamic cross-linked hydrogel to simulate the viscoelasticity and moisture regulation function of a natural nucleus pulposus, and the buffering and self-healing characteristics of the bionic intervertebral disc are achieved; the bionic fiber ring layer achieves gradient mechanical properties through directionally-arranged composite materials, and three-dimensional physiological movement of the artificial intervertebral disc close to the lumbar vertebra of a human body is achieved. The bionic intervertebral disc provided by the invention is matched with viscoelasticity of a natural intervertebral disc, recovers natural matching with three-dimensional physiological movement of a human spine, can buffer dynamic load and maintain intervertebral height, can perform micro-injury self-repair through dynamic crosslinking of chemical bonds in bionic nucleus pulposus, and has a good application prospect. And the kinematic mechanical stability of long-term service in a human body is realized.

The invention discloses a preparation method and application of bamboo nanocellulose and bi-crosslinking hydrogel. The preparation method of the hydrogel comprises the following steps: (1) dispersing the bamboo nanocellulose; (2) preparing a lignin-Fe solution; and (3) preparation of the bi-crosslinked hydrogel: adding the bamboo nanocellulose suspension and acrylic acid into water, then adding ammonium persulfate and N, N '-methylene bisacrylamide, finally adding the lignin-Fe solution to obtain a precursor solution, and pouring the precursor solution into a mold to obtain the bi-crosslinked hydrogel. The moso bamboo is used as a raw material, cellulose is extracted from the plant which is extremely high in carbon sequestration capacity and rapid in production and then is processed into nano cellulose, a novel utilization way is provided for the moso bamboo, and the hydrogel prepared from the moso bamboo is good in environment friendliness, green, natural and non-toxic, can be in direct contact with skin, and has a good application prospect. And the application and development of the material in the fields of environmental protection, biomedical treatment, electronic skin, wearable sensors and the like are facilitated.

In some aspects, the present disclosure provides systems for forming hydrogels that comprise (i) a reactive multi-arm polymer that comprises three or more polymer arms linked to a core region, each arm comprising a polyether segment and a reactive moiety comprising a cyclic imide carboxy-C1-C6-alkyl ester end group or each arm comprising a polyether segment and a reactive moiety comprising a C2-C6-isocyanoalkyl end group and (ii) a polyamino compound comprising at least two amino (—NH2) groups, wherein the reactive multi-arm polymer and the polyamino compound react to form a crosslinked hydrogel that does not contain ester groups and has long-term stability in vivo. In some aspects, the present disclosure provides methods of treatment using such systems and hydrolysis-resistant crosslinked hydrogels formed from such systems.

This disclosure provides a wearable electrochemical biosensor for detecting at least one target analyte in interstitial fluid of a subject, comprising, a microneedle array comprising a highly- crosslinked hydrogel polymer; at least one electrochemical chip comprising at least one transducer surface configured for generating a signal in the presence of the at least one target analyte; and a low crosslinked hydrogel polymer, wherein the low crosslinked hydrogel polymer is configured to couple the microneedle array with the at least one transducer surface of the electrochemical chip. Methods of making and use thereof are also disclosed.

The invention relates to the technical field of hydrogel, in particular to photocuring 3D printing ink and a preparation method thereof, synchronous curing bi-crosslinking hydrogel and application thereof. According to the invention, based on the characteristic that an initiator (persulfate) commonly used in a hydrogel preparation process has strong oxidizing property and can react with reducing metal ions to generate free radicals, Fe < 2 + > released by a cationic photoinitiator containing Fe < 2 + > in ultraviolet light reacts with persulfate to generate Fe < 3 + > and free radicals; the preparation of the water-soluble anionic polymer and water-soluble vinyl monomer double-network cross-linked hydrogel is synchronously realized. According to the invention, the problems of insufficient conductivity, stretchability and flexibility of hydrogel prepared by utilizing photocuring 3D printing are solved.

The present invention relates to the field of medical repair materials and particularly to a double-crosslinked hydrogel composite material, a preparation method therefor, and use thereof. The double-crosslinked hydrogel composite material comprises a physical-chemical double-crosslinked copolymer formed by reacting at least one type of acrylic monomer and at least one type of zwitterionic monomer in the presence of nanoparticles and a crosslinking agent. The double-crosslinked hydrogel composite material of the present invention has the characteristics of high mechanical strength, high adhesion strength, and swelling resistance, is low in cost, and features a preparation process that is simple and rapid and easy to regulate. Therefore, the material has very high application value.

In some aspects, the present disclosure provide systems for forming crosslinked reaction products, which comprise (a) a first composition that comprises a first multifunctional molecule that comprises a plurality of first reactive moieties that comprise oxyamine groups and (b) a second composition that comprises a second multifunctional molecule that comprises a plurality of second reactive moieties that comprise aldehyde or ketone groups, wherein at least one of the first and second multifunctional molecules is a multi-arm polymer and wherein at least one of the first and second multifunctional molecules comprises one or more covalently attached iodine groups. Other aspects of the present disclosure pertain to crosslinked networks that are formed using such systems and methods of treatment using such systems.

The present invention discloses a self-degradable double-crosslinked hydrogel temporary plugging agent, a preparation method and application thereof, and belongs to the technical field of oil and gas extraction. The self-degradable double-crosslinked hydrogel temporary plugging agent of the present invention, based on the total mass of water, the raw materials for preparing the self-degradable double-crosslinked hydrogel temporary plugging agent include the following components in percentage by mass: 4%-8% acrylamide, 0.5%-0.8% initiator, 0.2%-0.6% polyethylene glycol-diacrylate and polydopamine dispersion; the polydopamine dispersion is obtained by dissolving dopamine hydrochloride in an alkaline aqueous solution and polymerizing it. The temporary plugging agent of the present invention solves the problems of uncontrollable gel breaking time, narrow adjustment window and low gel strength of the single ester-based crosslinking system. The strongly reducing free dopamine monomer not only inhibits the polymerization of acrylamide monomer and prolongs the gel time, but also shortens the length of the polyacrylamide molecular chain after gelation, and reduces the viscosity of the solution after the gel is spontaneously degraded.

The invention discloses a photosensitizer, dynamic antibacterial hydrogel containing lipidosome wrapping the photosensitizer and a preparation method and application of the dynamic antibacterial hydrogel, and belongs to the technical field of biological materials. The invention provides a novel D-A-D type photosensitizer TPT which is simple in structure and simple in synthetic route, and from the angles of safety, practicability and use specificity of hydrogel dressing, modified hyaluronic acid and modified carboxymethyl chitosan are used as raw materials, cationic liposome wrapping the novel photosensitizer TPT is carried, and the hydrogel dressing is prepared. The efficient antibacterial dynamic cross-linked hydrogel material is prepared. The hydrogel disclosed by the invention has two pH response bonds, namely an imine bond and a boric acid ester bond, and has good moisture retention and biocompatibility; meanwhile, the hydrogel is loaded with the cationic liposome wrapping the novel photosensitizer TPT, so that the hydrogel has excellent active oxygen yield. The hydrogel disclosed by the invention aims to quickly resist bacteria, promote wound healing, reduce infection risk and improve the success rate of treatment through the targeted drug release function of the intelligent hydrogel.

In certain embodiments, the present disclosure provides medical graft devices having covalently crosslinked matrix protein hydrogel coatings. In some forms, such coatings are configured to reduce the formation of adhesions upon implantation.

The application relates to the technical field of medical materials, and discloses a functionalized hydrogel for treating atopic dermatitis and a preparation method and application thereof, the preparation method comprising the following steps: taking g-C3N4 powder as raw material to prepare C3N4 gel with a three-dimensional network structure; taking g-C3N4 powder and CDs powder as raw material, adding a matrix solution, and uniformly ultrasonically dispersing; adding a crosslinking agent solution to make the solution fully undergo crosslinking reaction to prepare a CDs / g-C3N4 crosslinking solution; pouring the above-mentioned CDs / g-C3N4 crosslinking solution into the above-mentioned C3N4 gel, standing for gelation, forming a functionalized modified carbon nitride gel three-dimensional network structure, and freeze-drying to obtain a functionalized three-dimensional crosslinking hydrogel. The functionalized three-dimensional crosslinking hydrogel prepared by the application has the advantages of degradability and the ability to penetrate through an intact skin barrier; and can be used for treating atopic dermatitis.

According to the hydrogel material disclosed by the invention, the gelatin / gallic acid conjugate with a specific concentration is taken as a base material, and the base material is cross-linked by utilizing a horse radish peroxidase / hydrogen peroxide combined enzyme catalysis system, so that an additional photo-thermal response material does not need to be added in the gelling process of the hydrogel; in the cross-linking process, the hydrogel serves as a cross-linked structure for forming the interior of the hydrogel and shows excellent photo-thermal responsiveness, and the hydrogel can respond to photo-thermal temperature rise to improve the self deformability, so that irregular wounds are better covered; therefore, the invention provides a hydrogel material which has good biological safety, oxidation resistance and bacteriostasis and can be used for wound injury repair.

A bioerodible crosslinked hydrogel based on a multi-arm polyoxazoline having a cage-like silicon-oxygen core is provided. In some aspects, the disclosure relates to reactive multi-arm polymers having a silicon-oxygen cage core and a plurality of polyoxazoline-containing arms extending from the core, the polyoxazoline-containing arms comprising a first end covalently bonded to the silicon-oxygen cage core and a second end comprising a moiety comprising a reactive end group. In other aspects, the disclosure relates to systems comprising such reactive multi-arm polymers and multifunctional compounds comprising functional groups reactive with the reactive end groups of the reactive multi-arm polymers. Other aspects relate to medical hydrogels formed by crosslinking such reactive multi-arm polymers with such multifunctional compounds, and to methods of treatment comprising administering to a subject a mixture comprising such reactive multi-arm polymers together with such multifunctional compounds.