196results about How to "Affect activity" patented technology

Rationally-designed LAGLIDADG (SEQ ID NO: 37) meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

A preservative and flavoring system for tea based beverages. The system contains a tea extract, an antimicrobially active amount of cinnamic acid or an acidic derivative thereof and an acidulant in an amount that maintains the pH of the beverage below pH 4.5. The system preferably includes an additional preservative such as a weak acid preservative. It can be used to prepare an ambient-stable tea based beverage and is suitable for cold filling.

The present invention relates to dietary supplement phytochemical compositions, comprising calcium, potassium double salt of (−)-hydroxycitric acid and glucosamine hydrochloride, and optionally boswellic acids, curcuminoids, 5-hydroxytryptophan, chondroitin sulfate and L-carnitine. The claimed compositions are useful in dietary supplements, nutritional supplements or pharmaceutical preparations for weight loss and inflammatory epidemics.

The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.

Targeted transcriptional effectors (transcription activators and transcription repressors) derived from meganucleases are described. Also described are nucleic acids encoding same, and methods of using same to regulate gene expression. The targeted transcriptional effectors can comprise (i) a meganuclease DNA-binding domain lacking endonuclease cleavage activity that binds to a target recognition site; and (ii) a transcription effector domain.

Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Poly(ADP-ribose) polymerase-1 (PARP-1) is a central signaling enzyme in a cell nucleus. PARP-1 is a target for the development of radio and chemo sensitizing agents in cancer treatment as well as providing protection from stroke. An SH3 domain and an SH3 ligand domain have now been discovered on the PARP-1 protein. These domains are involved in PARP-1 activation. This discovery makes possible the use of bioinformatics tools for the design of new drugs and strategies for drug target selection, specifically targeting the PARP-1 enzyme.

The invention relates to a method for processing micro-polluted water. The method utilizes the technology of integrating a membrane bioreactor with coagulating sedimentation and biological denitrogeneration, overcomes the defects of the processing technology of a conventional membrane bioreactor, realizes stable treatment effect, can simultaneously remove organic substances, nitrogen, phosphor and chromaticity in the water, and has the good effect of wide application range.

The invention discloses a method and a system for assisting the safe driving of a driver, and a mobile terminal. The method comprises the steps of starting a driving mode, transferring the mobile terminal to a silent mode; when receiving an incoming call or a short message, judging that whether the calling user of the incoming call or the short message exists in a prestoring database; and if yes, closing the silent mode of the mobile terminal, voice broadcasting the name of the calling user of the incoming call or voice reading the short message, and otherwise, continuing keeping the mobile terminal in the silent mode. According to the method and the system for assisting the safe driving of the driver, and the mobile terminal provided by the invention, the driver only can answer the calls of important contacts in driving, thus the disadvantage that the life and working of the user are influenced by missing the important calls or short messages is avoided; and on the other end, the incoming calls or short messages from most contacts are set to the silent mode, thus the disadvantage that the driving of the driver is influenced by the excessive incoming calls or short messages is avoided.

The invention discloses a traditional Chinese medicine composition for regulating blood lipids, blood pressure and blood glucose and a preparation method thereof. The composition is prepared from the following raw materials and auxiliary materials in parts by weight: 10-15 parts of gynostemma pentaphylla, 5-9 parts of broadleaf holly leaf, 5-9 parts of folium cortex eucommiae, 5-9 parts of folium mori, 5-9 parts of oriental wormwood, 3-6 parts of selfheal, 3-6 parts of hawthorn, 3-6 parts of persimmon leaf, 3-6 parts of the root of kudzu vine, 1-4 parts of Chinese yam, 1-4 parts of cyrtomium fortunei, 1-4 parts of radix achyranthis bidentatae, 1-4 parts of semen cassiae, 1-4 parts of rheum officinale, and 1-4 parts of red flower. The traditional Chinese medicine composition has the advantage that gynostemma pentaphylla taken as the main medicine is combined with other traditional Chinese medicines, thereby playing a synergistic effect. Pharmacodynamic tests and clinical observation prove that the composition has the characteristics of becoming effective rapidly and having small toxic and side effects in regulating blood lipids, blood pressure and blood glucose.

The invention discloses a method of replacing 10kV distribution transformer, the method spans a full insulated bypass system which is shunt-wounded with the original high voltage and low voltage conversion system, the full insulated bypass system can replace the original system when the original high voltage and low voltage conversion system is cut to replace the distribution transformer, when the replaced new distribution transformer runs normally, the full insulated bypass system is backouted, the method can replace the distribution transformer which is to be replaced without cutting off load of low voltage side and power off, it do not affect life and production of client.

Polypeptides with tubulin acetyltransferase activity are described, as are nucleic acids encoding said polypeptides, and methods of use. The invention further provides enhancers and inhibitors of tubulin acetyltransferase activity, as well as cells having altered tubulin transferase activity.

The invention relates to a Chinese herbal medicine clematis drying equipment that is heated evenly and can be weighed in real time. It includes a heat drying box, a clematis fixing groove, a base and a hot air box. The top of the guide rail is vertically installed with a rotating shaft through an electromagnetic slider, fixed cross bars are installed on both sides of the turntable, and the top of the fixed cross bar away from the end of the turntable is equipped with a clematis fixing slot, and the middle position of the top of the base is There is a heat drying box for welding. The present invention installs a pressure sensor at the joint between the clematis fixing groove and the fixing cross bar, so that when the clematis is drying, the weight of the clematis inside each clematis fixing groove can be known at any time, without subsequent labor It is very convenient to carry out weighing, which greatly improves the working efficiency.

The invention discloses a method for treating organic waste gas in a chemical plant. The organic waste gas sequentially pass through a flowmeter, a flow regulating valve, a concentration monitor, a dust removal flame-retardant device, an air inlet fan, a heat exchanger, a catalyst chamber, an exhaust fan, an alkaline tower, a humidifier, a biological active packing layer containing scrap iron, a 1# electrode, a 2# electrode, a 3# electrode, a 4# electrode, a circulating water system, a thermostat, a spraying system, a three-way valve and a biological filter. According to the method, the concentration of the organic waste gas can be regulated into a proper concentration range of the waste gas treatment system. According to the method, different waste gas treatment methods can be selected according to the concentration of the organic waste gas. In the biological treatment section of the method, acidic substances in the waste gas are removed by adopting an alkali washing method, and the condition that the biological activity is influenced in the biological filter is avoided; and in the biological treatment section of the method, the scrap iron is contained in the packing, the packing is stirred periodically by utilizing the variable electrode magnetism, the phenomena that the gas flow is influenced due to blockage and the reaction is non-uniform are avoided, a stirrer can be replaced, and the inner reaction space is saved.

The invention proposes a ultrahigh-speed magnetic suspension solid-liquid separation device. A stator of a disc motor is fixedly connected to the center position of the top of a shell; the top of the shell is fixedly connected with an axial electromagnetic coil; the side surface of the shell is fixedly connected with a side electromagnetic coil; a first opening is formed in the bottom of the shell; a rotating bin is positioned in the shell; the center position at the top of the rotating bin is fixedly connected with a rotor of the disc motor; the top of the rotating bin is fixedly connected with a top permanent magnet having a magnetic field effect with the axial electromagnetic coil; the side surface of the rotating bin is fixedly connected with a side permanent magnet having a magnetic field effect with the side electromagnetic coil; a second opening is formed in the bottom of the rotating bin; and a nozzle is positioned in the rotating bin. The ultrahigh-speed magnetic suspension solid-liquid separation device has the beneficial effects of high separation rotating speed, easy adjustment of the separation rotating speed, prevention of the temperature rise, no solution pollution, no motor corrosion, simple procedures, higher efficiency and lower cost.

The invention relates to the field of automobile accessories, in particular to an automobile exhaust purifier capable of automatically removing coarse carbon particles. The automobile exhaust purifiercomprises a left outer cylinder, a right outer cylinder and a honeycomb carrier, the left outer cylinder and the inner wall of the right outer cylinder communicate and are connected detachably, innercylinders are installed in the left outer cylinder and the right outer cylinder, and the honeycomb carrier is arranged between the two inner cylinders. Honeycomb holes of the honeycomb carrier are internally coated with catalyst layers. Electromagnets and outer magnets are arranged on the inner cylinders and in a cavity of the left outer cylinder and a cavity of the right outer cylinder, an innermagnet is arranged in each inner cylinder, a coarse carbon filter net is arranged on each inner magnet, and the inner magnets and the outer magnets correspond to each other in position. Coarse carbonparticles on the inner cavities and the coarse carbon filter nets can be automatically removed, the coarse carbon particles are prevented from affecting the activity of catalysts after being broughtinto a catalytic device, meanwhile, the catalytic device can be rapidly disassembled to remove the coarse carbon particles, and the catalyst activity of the catalytic device is ensured, and hydrocarbon, NOx, carbon monoxide and the like in automobile exhaust can be better purified.

The invention relates to a proteolytic enzyme which is capable of forming fibrin when it reacts with fibrinogen, a fibrin-glue kit and a fibrin-glue formulation comprising an enzymatically-permissive concentration of a visualization agent and to their use in methods for prevention and/or reduction of adhesions and/or methods for promotion of blood coagulation sealing or filling body surfaces.

The invention relates to the technical field of agricultural bactericide preparations, in particular to a young garlic shoot preservative, which comprises natamycin, epsilon-polylysine, an antioxidant, a chelant and a mixture of other aids. The young garlic shoot preservative is applied to storing and refreshing young garlic shoot, and can pertinently inhibit the growth of molds in the storage of the young garlic shoot and reduce the use of a chemical preservative.

The invention provides a kit for quantitatively detecting a heart-type fatty acid binding protein. The kit comprises a heart-type fatty acid binding protein calibrator, a magnetic particle coupled with an anti-biotin antibody, an acridine derivative-labeled heart-type fatty acid binding protein monoclonal antibody, a biotin-labeled heart-type fatty acid binding protein monoclonal antibody, a chemiluminescent substrate and a quality control substance. A preparation method of the kit comprises the following steps: preparing the calibrator from a pure heart-type fatty acid binding protein raw material; preparing the acridine derivative-labeled antibody; preparing a biotinylated antibody; coupling a magnetic particle with the anti-biotin antibody; sub-packaging the calibrator, a marker mixturesolution and the chemiluminescent substrate; and performing assembling to obtain the finished product. The kit has the advantages of high sensitivity, good specificity, high accuracy of a quantitative detection result, low use cost, and easiness in promotion and application.

The invention relates to a bio-membrane reactor realizing synchronous nitrification, denitrification and anaerobic ammoxidation and a fast realization method. The interior of a reactor body is provided with a spiral hollow fiber membrane tube, the hollow fiber membrane tube extends from the upper part of the reactor body to the lower part of the reactor body along the central axis of the reactor body, a space spirally surrounded by the hollow fiber membrane tube is internally provided with a non-woven packing, and the edge of the non-woven packing is 0.5-3 cm away from the hollow fiber membrane tube; one end of the spiral hollow fiber membrane tube is closed, and the other end is externally connected with an aeration system. The bio-membrane reactor successfully makes a coupling reaction start up after running for 80-160 days, and efficiently removes nitrogen and organic matters in sewage; the method can realize synchronous nitrification-denitrification-anaerobic ammoxidation reaction in the same reactor, effectively shortens the start-up time, moreover, simplifies the process flow, and saves the operation cost.

The invention discloses a preparation method of novel tissue engineering skin. The method includes steps of jointly culturing an amniotic membrane decellularized scaffold and a mesenchymal stem cell to be a carrier, inoculating horn cell; culturing by a serum-free culturing system, and acquiring a complete tissue engineering skin membrane. Through the method, the method can culture a complete skin membrane and the skin membrane can be applied to the skin scar repair and leucoderma treatment. The horn cell is originated from body skin or prepuce of the newborn. The carrier is low in immunogenicity while also has biological activity; the serum-free culturing system can realize the in-vitro serum-free cultivation and amplification of the horn cell, and improve cell growth, multiplication, cell form, activity and function instead of bad influence; the skin membrane cultivated by the method is approached to natural skin; after the skin-grafting culturing, it can be applied to the scar repair of scaled and other injured skins as well as treatment of skin disease such as leucoderma.

Disclosed are methods and compositions for identifying, producing, and using pathologically-activated targeting compounds. Pathologically-activated compounds are compound that only have an effect, or have a disproportionate effect, on a target molecule when a pathological condition exists.