Heating and blending instrument suitable for plaque forming cell determination agar solid phase method
By designing a heating mixer, the eccentric turntable and electric heating coil are used to achieve uniform heating and mechanical shaking of agarose, which solves the problem of agarose solidification and uneven shaking of manual shaking at high temperatures, and improves the accuracy of experimental results.
Patent Information
- Application Number
- CN202420670990.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-02
- Publication Date
- 2025-01-10
- Estimated Expiration
- 2034-04-02
AI Technical Summary
In the agar solid phase method for plaque-forming cells, agarose is inhomogeneously insulated in a high-temperature water bath, which leads to solidification, which affects the experimental results, and is shaken and mixed evenly, affecting the accuracy of the test results.
A heating mixer is designed, including an eccentric turntable and a shaking turntable, equipped with an electric heating coil and thermal insulation layer to achieve uniform heating of agarose and mechanical shake, ensuring the temperature is controlled at 45-50℃, and it is reduced by mechanical shake. Manual mixing error.
It effectively avoids the solidification of agarose, ensures temperature uniformity, and achieves a better mixing effect through mechanical shake, making the experimental results more accurate.
Smart Images
Figure CN222324946U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of immunogenicity evaluation of medical devices, and in particular relates to a heating and mixing instrument suitable for agar solid phase method for measuring plaque-forming cells. Background Art
[0002] Among the methods for evaluating the immunogenicity of medical devices, the agar solid phase method for plaque-forming cell determination is used to evaluate the effects of medical devices / materials on the body's humoral immune function. There is a key step in this method, which is to quickly mix 1% agarose with 0.2mL of 10% SRBC (sheep red blood cells) and spleen cell suspension, and then add it to a 6-well plate and shake it quickly to spread the suspension on the bottom of the 6-well plate. The agarose after high-temperature sterilization needs to be kept warm in a water bath at 45-50°C in the standard. If the seal is not good after packaging, it is easy to cause water to enter the test tube and cannot be used for subsequent experiments. At the same time, the temperature of the water bath is not easy to control, which can easily cause uneven heating of the agarose, resulting in coagulation of the agarose, affecting the experimental results. If the temperature is increased, when mixing the cell suspension and SRBC, the activity of the spleen cells will be affected due to the high temperature, thereby affecting the experimental results; finally, when shaking and mixing the 6-well plate, manual shaking is used, and there are large differences in each shaking, which affects the observation of the experimental results. Utility Model Content
[0003] The technical problem to be solved by the utility model is to provide a heating mixer suitable for agar solid phase method for plaque forming cell determination, which can effectively keep agarose warm to prevent it from solidifying and has a good mixing effect.
[0004] The technical scheme adopted by the utility model is: a heating and mixing instrument suitable for agar solid phase method for determining plaque forming cells, comprising a workbench, a driving motor is installed in the workbench, an eccentric turntable is arranged at a position corresponding to the driving motor on the surface of the workbench, the bottom circle center of the eccentric turntable is connected to the driving shaft of the driving motor, a vertical eccentric shaft is formed on the top surface of the eccentric turntable, and a power supply connector is arranged at the top center of the eccentric turntable, a shaking turntable is arranged on the top surface of the eccentric shaft, a connecting hole matching the eccentric shaft is opened at the bottom center of the shaking turntable, and a connecting groove is opened at the bottom of the shaking turntable corresponding to the power supply connector, an electric heating coil matching the power supply connector is arranged in the connecting groove, corresponding grooves are opened on the shaking turntable corresponding to the porous cell culture plate, each groove is matched with each downwardly protruding hole of the porous cell culture plate in a one-to-one correspondence, the shaking turntable is a hollow structure, the electric heating coils are respectively extended to the outer bottom surface of each groove to form a plurality of small coils, and a heat preservation layer is arranged around the shaking turntable on the workbench.
[0005] Preferably, an operation panel is provided obliquely on one side of the workbench.
[0006] Preferably, the thermal insulation layer is an electric heating plate.
[0007] Preferably, a transparent cover matching the enclosed area is provided on the workbench in the area enclosed by the thermal insulation layer.
[0008] The beneficial effects of the utility model are:
[0009] (1) By setting an eccentric turntable and a shaking turntable, the eccentric turntable drives the shaking turntable to rotate eccentrically, so that the shaking turntable produces reciprocating shaking, which can make the porous cell culture plate placed on the shaking turntable shake evenly, thereby achieving the purpose of shaking;
[0010] (2) By arranging an electric heating coil and a small coil in the shaking turntable, the grooves on the shaking turntable and the culture wells of the multi-porous cell culture plate are preheated and kept warm, and the sterilized agarose can be directly dispensed into the culture wells of the multi-porous cell culture plate. Due to the preheating and heat preservation of the bottom of the groove, the temperature of the small coil can be effectively controlled to be 45-50°C, thereby avoiding the coagulation of agarose at low temperature or the influence of high temperature on the activity of the spleen cells mixed subsequently;
[0011] (3) The shaking turntable is plugged into the eccentric turntable, and the shaking turntable with the corresponding number of holes can be selected according to different multi-porous cell culture plates;
[0012] (4) A heat preservation layer is arranged around the shaking turntable to further achieve the purpose of heat preservation.
[0013] The utility model is applied to the agar solid phase method for determining plaque-forming cells, can effectively ensure the temperature of agarose, and has a better mixing effect through mechanical shaking, so that the test result is more accurate. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a structural schematic diagram of the utility model.
[0015] In the figure: 1. workbench; 2. driving motor; 3. eccentric turntable; 4. eccentric shaft; 5. power connector; 6. shaking turntable; 7. connecting hole; 8. connecting groove; 9. electric heating coil; 10. groove; 11. small coil; 12. insulation layer. DETAILED DESCRIPTION
[0016] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
[0017] Example
[0018] like Figure 1As shown, the heating mixer suitable for the agar solid phase method for determining plaque-forming cells provided in this embodiment comprises a workbench 1, one side of the workbench 1 is an inclined surface, and an operation panel is installed on the inclined surface, a driving motor 2 is installed in the workbench 1, and the driving shaft of the driving motor 2 is vertically upward, and is connected to the bottom center of an eccentric turntable 3 after passing through the surface of the workbench 1, an eccentric shaft 4 is formed on the top surface of the eccentric turntable 3, and an upwardly protruding power connector 5 is also installed on the top surface of the eccentric turntable 3, the power connector 5 and the switch of the driving motor 2 are connected to the operation panel through a wire, and a shaking turntable 6 is provided on the top surface of the eccentric turntable 3. In this embodiment, a 6-well cell culture plate is taken as an example, and the shaking turntable 6 adopts a hollow structure, and 6 grooves 10 corresponding to the 6-well cell culture plate are evenly opened on the top surface of the shaking turntable 6, and the bottom of the groove 10 is close to the concave A small coil 11 is installed on the bottom surface of the groove 10, and a connecting hole 7 matching the eccentric shaft 4 is opened at the bottom center of the shaking turntable 6, and a connecting groove 8 is opened at the bottom of the shaking turntable 6 corresponding to the power connector 5. When the shaking turntable 6 is connected to the eccentric shaft 4 through the connecting hole 7, the power connector 5 extends into the connecting groove 8, and an electric heating coil 9 is installed in the connecting groove 8. When the power connector 5 extends into the connecting groove 8, the power connector 5 is connected to the electric heating coil 9. After turning on the power connector 5 switch on the operation panel, the electric heating coil 9 is powered. The small coils 11 are all extended and dispersed by the electric heating coil 9. Therefore, when the electric heating coil 9 is energized, the small coils 11 are energized and heated; an insulation layer 12 is provided around the shaking turntable 6 on the workbench 1, and the insulation layer 12 is an electric heating plate, and a transparent cover matching the enclosed area is provided in the area enclosed by the insulation layer 12 on the workbench 1.
[0019] When in use, taking a 6-well cell culture plate as an example, turn on the power on the operation panel to heat the electric heating plate of the insulation layer 12, and at the same time heat the small coil 11, so that the heating temperature of the insulation layer 12 and the small coil 11 is controlled at 45-50°C. After the small coil 11 is heated, it preheats the groove 10 of the shaking turntable 6. After the preheating is completed, the sterilized agarose is directly dispensed into the culture wells of the 6-well cell culture plate, and then the culture wells of the 6-well cell culture plate are directly corresponding to the grooves 10 of the shaking turntable 6 and then embedded and placed. At this time, the small coil 11 and the insulation layer 12 are powered on and heated. In the hot state, the agarose in the 6-well cell culture plate is kept warm to prevent the agarose from solidifying, and then the driving motor 2 is turned on through the operation panel, and the driving motor 2 drives the eccentric turntable 3 to rotate, and the eccentric turntable 3 drives the shaking turntable 6 to make eccentric circular motion. At this time, the cell suspension and SRBC are added to the 6-well cell culture plate, so that the purpose of mixing is achieved while adding the sample, reducing the error of manual mixing, and making the test result more accurate. After the sample addition and mixing are completed, the 6-well cell culture plate is taken out, placed at room temperature until the agar solidifies naturally, and then placed in an incubator for culture.
[0020] The above is only a preferred implementation of the present invention, but the protection scope of the present invention is not limited thereto, and any modification and replacement based on the technical solution and inventive concept provided by the present invention should be included in the protection scope of the present invention.
Claims
1. A heating and mixing apparatus suitable for agar solid phase method for plaque forming cell assay, characterized in that: The invention comprises a workbench (1), wherein a driving motor (2) is installed in the workbench (1), an eccentric turntable (3) is arranged at a position on the surface of the workbench (1) corresponding to the driving motor (2), the bottom center of the eccentric turntable (3) is connected to the driving shaft of the driving motor (2), a vertical eccentric shaft (4) is formed on the top surface of the eccentric turntable (3), and a power supply connector (5) is arranged at the top center of the eccentric turntable (3), a shaking turntable (6) is arranged on the top surface of the eccentric shaft (4), a connecting hole (7) matching the eccentric shaft (4) is opened at the bottom center of the shaking turntable (6), and the shaking turntable (6) A connection groove (8) is provided at the bottom corresponding to the power connector (5), and an electric heating coil (9) matched with the power connector (5) is provided in the connection groove (8). A corresponding groove (10) is provided on the shaking turntable (6) corresponding to the multi-porous cell culture plate, and each groove (10) is matched with each downwardly protruding hole of the multi-porous cell culture plate in a one-to-one correspondence. The shaking turntable (6) is a hollow structure, and the electric heating coil (9) is respectively extended to the outer bottom surface of each groove (10) to form a plurality of small coils (11). A heat preservation layer (12) is provided around the shaking turntable (6) on the workbench (1).
2. The heating and mixing apparatus suitable for the agar solid phase method for determining plaque forming cells according to claim 1, characterized in that: An operating panel is obliquely provided on one side of the workbench (1).
3. The heating and mixing apparatus suitable for the agar solid phase method for determining plaque forming cells according to claim 1 or 2, characterized in that: The thermal insulation layer (12) is an electric heating plate.
4. The heating and mixing apparatus suitable for the agar solid phase method for determining plaque forming cells according to claim 3, characterized in that: A transparent cover body matching the enclosed area is also provided on the workbench (1) in the area enclosed by the thermal insulation layer (12).