Gene therapy for the regeneration of chondrocytes or cartilage type cells

A chondrocyte and cell technology, applied in gene therapy, cells and microorganisms modified by the introduction of foreign genetic material, etc.

Pending Publication Date: 2016-06-01
FIGENE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no general scientific evidence to support the use of gene therapy in cartilage regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Gene therapy for regeneration of chondrocytes or chondrocytes

[0173] An individual in need of regeneration of chondrocytes or chondrocyte-type cells is a subject of one or more methods of and / or exposed to one or more compositions of the present disclosure. The individual may be at risk of requiring regeneration of chondrocytes or chondrogenic cells, or the individual may be diagnosed as requiring regeneration of chondrocytes or chondrogenic cells. Individuals at risk for needing chondrocytes or chondrogenic cell regeneration may be at risk for any reason, including becoming or having been athletes, individuals with joint injuries, obese individuals, those whose occupation or lifestyle requires physical exertion, including past Lifting too much, or predisposed to have a medical condition that deleteriously affects joints or cartilage (eg, such as having a family history or having one or more known markers and / or risk factors for susceptibility to the medical condition...

Embodiment 2

[0177] Examples of Materials and Methods

[0178] In certain embodiments, the following methods are used in the present disclosure.

[0179] Acquisition and expansion of human Wharton's glial cells

[0180] Human Wharton's jelly cells (hWJC) were isolated from human umbilical cord Wharton's jelly (Devarajan et al., 2013) according to our previously published protocol and collected after notification (KU-IRB#15402). hWJCs were cultured in conventional hWJC medium (10% fetal bovine serum (FBS-MSCQualified) and 1% penicillin-streptomycin in low glucose DMEM (Life Technologies, Carlsbad, CA)). The hWJC medium was changed three times a week and the hWJC were incubated in a cell culture grade incubator at 37 °C and 5% CO 2 down to maintain. hWJCs were snap frozen at passage 2 (P2) until needed for experiments. hWJCs were thawed and expanded from P2 to P5 before being used for experiments. All experiments were performed in triplicate for each umbilical cord.

[0181] plasmid

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PUM

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Abstract

Embodiments of the invention concern the regeneration of chondrocytes and cartilage-type cells. In certain embodiments, one or more genes are employed for the regeneration of chondrocytes and ca1iilage-type cells. In particular embodiments, one or more gene therapy regimens are employed for the regeneration of chondrocytes and cartilage-type cells. In particular aspects, embodiments concern cartilage repair, such as articular cartilage repair. More particularly, embodiments for the disclosure concern using gene therapy for the attraction, generation and / or regeneration of chondrocytes or other cartilage-type cells and / or the generation and / or repair of cartilage tissue. In specific embodiments of the disclosure, gene therapy is provided that is capable of attracting and / or generating desired cells in vivo.

Description

[0001] This application claims priority to US Provisional Patent Application Serial No. 61 / 875,509, filed September 9, 2013, which is hereby incorporated by reference in its entirety. field of invention [0002] The present invention relates generally to at least the fields of medicine, surgery, anatomy, biology, cell biology and / or molecular biology. Background of the invention [0003] cartilage regeneration [0004] Injuries to joints and articular cartilage are common; more than 6 million people in the United States see a doctor each year for a variety of knee, wrist, and ankle problems. Progressive wear and tear of the articular cartilage can lead to progressive loss of cartilage tissue, further exposing the ends of the bones and leaving them unprotected. This eventually deteriorates into the most common form of arthritis - osteoarthritis (or degenerative joint disease). [0005] Osteoarthritis has been reported to affect 33.6% (12.4 million) of adults 65 years of age...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00
CPCA61K48/0075C12N15/85C12N2800/107A61P19/02C12N5/10A61K48/0058
Inventor P·奥西伦
Owner FIGENE
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