179 results about "Cartilaginous Tissue" patented technology

A stitching device useful for implanting suture in tissue, for example, cartilaginous tissue. The device has a frame and an elongated tubular member mounted thereto. A suture capture needle having a suture capture opening extends from a distal end of the tube. A needle cannula having a lumen is slidably mounted in a passage of the tubular member. Actuation of a trigger member pivotally mounted to the frame moves a distal end of the cannula needle and a section of suture contained in a lumen in the cannula needle through the suture capture opening causing the suture section to be retained in the opening.

The present invention is a system for a partially biological disc replacement that stimulates natural fibrous, cartilaginous or other tissue growth in the DDD cavity, resulting in a partial biological disc replacement. Multiplicities of fibronous pieces of fibro-cartilaginous tissue promoting material are inserted into the DDD cavity inducing tissue growth. The fibro-cartilaginous tissue

A cartilaginous tissue repair device with a biocompatible, bioresorbable three-dimensional silk or other fibre lay and a biocompatible, bioresorbable substantially porous silk-based or other hydrogel partially or substantially filling the interstices of the fibre lay; with or without an integral means of firmly anchoring the device to a patient's bone.

A tap drill for dental implant for forming a space for coupling a fixture of the implant in an alveolar bone, includes: a shank part coupled with a torque transferring mechanism; and a tap part connected with the shank part, the tap part having: a tapping screw thread formed on the outer peripheral surface thereof for forming a space to insert and fix a fixture of the dental implant thereto; and cutting blades radially formed from the center of a front end face thereof, which is perpendicular to a rotary axis of the tap drill, whereby the tap drill can simultaneously perform a tapping work, on the outer peripheral surface of the tap part, against a cartilaginous tissue or an alveolar bone of a patient's body, and a cutting work on the front end face of the tap part while the tap part is rotated by the torque transferring mechanism.

The present invention provides a method for expanding a population of chondrocytes that maintains chondrocyte phenotype during the expansion by culturing the population in a defined serum-free expansion medium containing one or more cytokines and under low attachment conditions. The method further solves diffusion problems during the subsequent stage of extracellular matrix production by use of a perforated polycarbonate substrate that results in a randomly organized cultured neocartilage tissue. Chondrocytes expanded and cultured in this manner can be used in various medical applications to repair cartilaginous tissues that have been injured by trauma or disease.

The present invention provides a method for expanding a population of chondrocytes that maintains chondrocyte phenotype during the expansion by culturing the population in a defined serum-free expansion medium containing one or more cytokines and under low attachment conditions. The method further solves diffusion problems during the subsequent stage of extracellular matrix production by use of a perforated polycarbonate substrate that results in a randomly organized cultured neocartilage tissue. Chondrocytes expanded and cultured in this manner can be used in various medical applications to repair cartilaginous tissues that have been injured by trauma or disease.

Methods and devices for the regulation of matrix metalloproteinase gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for reduction of matrix metalloproteinase gene expression and exposing cartilage tissue to the fields generated by specific and selective signals so as to regulate matrix metalloproteinase gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, cartilage defects, and tumor metastasis.

Defects in a cartilaginous tissue are filled by: (a) mixing (i) a first reagent composition preferably comprising an amine-free hydroxyalkyl (preferably hydroxymethyl) phosphine crosslinking agent with (ii) a second reagent composition comprising a bioelastic polymer, the bioelastic polymer preferably comprising elastomeric units, the elastomeric units preferably selected from the group consisting of bioelastic pentapeptides, tetrapeptides, and nonapeptides; to produce a therapeutic composition; and then (b) administering the therapeutic composition to the cargilagenous tissue. Compositions and kits for carrying out the method are also described.

A stitching device useful for implanting suture in tissue, for example, cartilaginous tissue. The device has a frame and an elongated tubular member mounted thereto. A suture capture needle having a suture capture opening extends from a distal end of the tube. A needle cannula having a lumen is slidably mounted in a passage of the tubular member. Actuation of a trigger member pivotally mounted to the frame moves a distal end of the cannula needle and a section of suture contained in a lumen in the cannula needle through the suture capture opening causing the suture section to be retained in the opening.

The invention provides a compound betamethasone suspension injection, which is a compound preparation composed of low-solubility betamethasone dipropionate and high-solubility betamethasone sodium phosphate. Long-lasting, it can be used for the treatment of musculoskeletal and cartilage tissue diseases, allergic diseases, skin diseases, collagen diseases, tumors and other clinical diseases.

The invention provides a tissue engineered bone-cartilage composite tissue transplant and a preparation method thereof, and belongs to the tissue engineering field. The method comprises the following steps: seed cells are implanted on a scaffold material and cultured in vitro to form the tissue engineered bone-cartilage composite tissue transplant; the seed cells are osteogenic stem cells; the scaffold material comprises an osteogenic part and a chondroblast part, and the osteogenic part is added with an osteoblast induction factor and modified by a type-I collagen in a preparation process; and the chondroblast part is added with a chondroblast induction factor and modified by a type-II collagen. Bone marrow mesenchymal stem cells are successfully induced into osteocytes and chondrocytes, and bone tissues and cartilage tissues are formed after the obtained transplant is transplanted in vivo. Satisfactory effect is obtained from the tissue engineered bone-cartilage composite tissue which is constructed by the transplant.

Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.

The invention discloses a new cartilaginous affinity polypeptide sequence and a screening method thereof. In the invention, according to an improved phage display technique, a cell and tissue layers combined extracorporeal screening method is applied to cartilaginous affinity polypeptide screening for the first time so as to screen out the polypeptide sequence DWRVIIPPRPSA with chondrocyte affinity and tissue specificity, and thus, the drawback of simple cell screening or tissue screening in the prior art is overcome, and the cellular affinity and the tissue specificity are well combined. The invention also relates to the application of the polypeptide in the preparation of a targeted PEI nano-carrier for the gene therapy of mediated articular cartilage diseases, which realize the decoration on the PEI carrier by the cartilaginous affinity polypeptide, offers the cartilaginous tissue specificity and the cellular affinity to the carrier, and realizes the targeted therapy to the cartilage. In addition, the invention takes the chondrocyte itself as the screening target, which has better targeted effect and clinical practical value compared with the mode of taking the matrixes around the chondrocyte as the screening target in the prior art.

An articular cartilage graft and a preparation method thereof are provided. The prepared articular cartilage graft is composed of a superficial layer, a middle layer and a deep layer from outside to inside; the thickness, shape and size of each layer are all matched with a cartilage injury part, and thus preoperative shaping is not required; after grafting, the articular cartilage graft has layer distribution corresponding to distribution of each layer of surrounding normal cartilages, is conducive to intercellular signal transmission, transduction and regulation, and can be better integrated with surrounding normal cartilage tissues; the articular cartilage graft has structure characteristics consistent with those of the natural cartilages, the collagen type II content is gradually decreased from the superficial layer to the deep layer, the GAG content is increased gradually from the superficial layer to the deep layer, and compression resistance and wear resistance are good; and chondrocytes in the cartilage graft are wrapped with an extracellular matrix, have low immunogenicity, allow generation of immunologic rejection to be avoided after grafting, can survive for a long term and exert functions, and improve cartilage repair long-term curative effects.

Methods and devices for the regulation of aggrecan gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up regulation or down regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for aggrecan gene expression and exposing cartilage tissue to the fields generated by specific and selective signals so as to regulate aggrecan gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, and cartilage defects.

The invention discloses a preparation method of a silk fibroin-poly(hydroxybutyrate-hydroxyvalerate) composite fiber membrane, which comprises the steps of: mixing a prepared silk fibroin solution with a poly(hydroxybutyrate-hydroxyvalerate) solution to prepare a spinning solution, and conducting electrostatic spinning and aftertreatment. Based on the electrostatic spinning method, the composite fiber membrane is prepared, and the fiber membrane has good biocompatibility of the silk fibroin and the excellent mechanical performance of the poly(hydroxybutyrate-hydroxyvalerate) simultaneously, is more applicable to the biomedical field, and is expected to be used as a material for engineering of skin, nerves and cartilaginous tissues. The prepared composite fiber membrane has higher porosity, good mechanical performance, good air permeability and moisture permeability and large liquid absorption amount, can be degraded in human body and can promote tissue repair; and the preparation method has simple process.

The present invention relates to a cytobiological degradable material compound, its preparation method and application. Said compound is formed from polybutylene terephthalate / polyethylene glycol / digolactic acid (PBT / PEG / OLA) ternary copolyester (PPOCP) three-dimensional porous cell scaffold and stem cell, and the described stem cell is planted on the PPOCP three-dimensional porous cell scaffold. Said invention can be used for preparing composite cell carrier graft to repair injury of several tissue organs of skin, fat, urethra, bladder, soft bone tissue, muscular tissue and muscle tendon, etc.

The invention relates to a hollow stent for inducing the regeneration and moulding of cartilage / bone tissues. The structure of the stent comprises an overall three dimensional hollow structure; and a plurality of holes in the surface. The hardness should meet the requirements of cartilage / bone tissues in the repaired parts. The stent should have a good biocompatibility and is made of a biologically degradable material. The biologically degradable material can be made into filling and repairing materials such as artificial auricular cartilage stents, artificial nose bridges, and the like, and can also be made into artificial bone stents with various sizes. The prepared stents with special structures can be combined with cultured cartilage / bone cells in vitro and then implanted into human body. Or, small cartilage blocks and cartilage sheets are filled into the hollow stents. The stents can be implanted into human body at first, and then cartilage / bone cells are injected into the stents. The inoculated cartilage / bone cells proliferate and pass down in the hollow three-dimensional stents, bones or cartilage tissues with the shape of the stents gradually form during the period, and tissues with similar functions and shapes can be obtained.

Device for the regeneration and prevention of degeneration of the cartilage and subchondral bone, the proliferation of chondrocytes and for blocking or reducing the fibroblastic evolution of chondrocytes and mesenchymal cells by means of electromagnetic waves comprising a device for generating a periodic signal u(t) and a power amplifier suitable for applying the signal u(t) to a pair of solenoids for the generation of an electromagnetic field M(t) addressed towards a portion of human / animal body containing cartilage. Setting means are provided for the generation of an electromagnetic field having intensity between 0.5 and 2 milliTesla, frequency between 37 and 75 Hz and period of application between 1 hour and 9 hours.

The invention discloses a PHBV (Poly (HydroxyButyrate-hydroxyValerate)) nano fiber support material, a preparation method and application thereof. The preparation method comprises the following steps of: (1) dissolving PHBV powder with the molecular weight of 3*105 in an organic solvent to compound a solution with the concentration of 7.5%, and then stirring for 3-5 hours in a water bath at 50 DEG C by using magnetic force; (2) after fully dissolving the PHBV, hermetically placing the solution; (3) after the solution fully forms hydrogel, taking the hydrogel out, adding acetone for replacing the organic solvent, and then replacing the acetone by using deionized water; and (4) and freezing and drying the PHBV hydrogel to obtain the PHBV nano fiber support material. The PHBV nano fiber support material has the advantages of low cost, simple preparation process, good biocompatibility, high porosity and controllable support structure and fiber morphology; and because the morphological structure of the PHBV nano fiber support material is similar to that of an extracellular matrix in cartilaginous tissue cells of a human body, the PHBV nano fiber support material can promote the adhesion, the propagation and the differentiation of cartilaginous cells and is very suitable for restoring and reconstructing cartilaginous tissues.

The invention discloses a preparation method of functional food having the effects of nourishing cartilaginous tissues and resisting joint inflammation. The functional food comprises the following raw materials in percentage by weight: 3 to 40 percent of manggis extract, 5 to 30 percent of marasmius androsaceus extract, 5 to 40 percent of D-glucosamine hydrochloride, 0.1 to 10 percent of chondroitin sulfate and 1 to 15 percent of collagen. The preparation method comprises methods for controlling proportional content of the raw material components, extracting active ingredients of two raw materials and preparing a preparation. The functional food preparation has the effects of effectively nourishing the cartilaginous tissues of human bodies, diminishing inflammation and relieving pain.

The invention provides a method for preparing cartilage tissues from artificial in-vitro sources by the aid of human urine cells in an in-vitro manner. The method includes steps of (1), a), reprogramming the human urine cells to generate induced pluripotent stem cells and directionally differentiating the induced pluripotent stem cells to obtain mesenchymal stem cells, or b), carrying out transdifferentiation to generate the mesenchymal stem cells; (2), cultivating the mesenchymal stem cells obtained at the step (1) in cartilage induced differentiation media to obtain the cartilage tissues from the artificial in-vitro sources. The method has the advantages that the cartilage tissues are constructed in the in-vitro manner and can be used for replacing damaged or lesion tissue cells, so that the purpose of repairing cartilage can be achieved, and the effective method is provided for constructing the cartilage tissues in the in-vitro manner and has huge application value.

The invention belongs to the technical field of medicine; in order to solve the problem that no efficient and safe cartilage siRNA delivery system is provided for limiting application of RNAi in treating cartilage diseases, the invention provides a cartilage siRNA liposome delivery system and a preparation method of the delivery system. The delivery system consists of lipidosome, a lipidosome modifier and a small nucleic acid drug, wherein the lipidosome is phosphatidylcholine, cholesterol or Dlin-KC2-DMA; the lipidosome modifier is a polyethylene glycol lipid conjugate; and the small nucleic acid drug is Ihh siRNA. According to the invention, a liposome nano based cartilage RNA interference delivery system is modified, and the formed composite has positive charges, is safe and small in volume, and can be distributed in a full-lamellar cartilage tissue. Carrier experiments verify that siRNA can be delivered into cartilage cells to successful knock out corresponding genes inside the cartilage cells, therefore a bottleneck of gene knockout in the cartilage tissue is broken through; and the siRNA, as a target gene, can be used for treating PTOA (post-traumatic osteoarthritis).

The invention discloses a 'method for preparing a decellularization cartilage matrix material', and belongs to the technical field of biological material preparation. The method comprises the following steps: (1) carrying out immobilization treatment on human or animal cartilage tissue in an immobilization agent; (2) washing the cartilage tissue after the immobilization treatment by using an appreciate solution, wherein the washing time can be more times; (3) carrying out decellularization treatment on the washed cartilage tissue in an alkali solution; (4) carrying out soaking washing treatment on the cartilage tissue after the decellularization treatment, wherein the immobilization agent is glutaraldehyde, formaldehyde or peracetic acid, the appropriate solution may be distilled water, and the alkali solution is selected from 4-15% NaOH or KOH solutions, and a washing liquid used in soaking washing treatment is normal saline. The decellularization cartilage matrix material prepared byusing the method has a porosity of 70-90% and a pore size of 12-67mu m.

The present invention makes available an optimal concentration of a hedgehog polypeptide for modulating growth and / or cartilage production by chondrocytes. The present invention allows for improvements in the culturing of chondrocytes to develop cartilaginous tissue ex vivo suitable for implantation to replace damaged or deteriorated cartilage in a patient.

The invention discloses a novel high-strength microgel composite hydrogel which comprises the following components in percentage by weight: 30-70% of 2-acrylamido-2-methylpropanesulfonic acid, 5-20% of acrylamide, 5-10% of N-hydroxymethyl acrylamide, 10-50% of water and 2-5% of nano clay. The composite hydrogel has the advantages of higher mechanical strength and high water absorptivity; the strength and water content of the composite hydrogel are similar to those of the human body cartilaginous tissue; and thus, the composite hydrogel is a replaceable material for cartilaginous tissue repair.

The present invention relates to an ultrasonic device for the treatment of arthritis, and more particularly, to an ultrasonic device for the treatment or prevention of degenerative arthritis, which is so structured that it generates ultrasonic waves with an intensity of 200-800 mW / cm2 and applies low-intensity ultrasonic energy of 100-400 mW / cm2 to cartilage cells deep in the skin's dermis. The inventive ultrasonic device comprises: a main control unit including an ultrasonic oscillation control unit allowing the generation of ultrasonic waves with a frequency of 0.1-5 MHz and low intensity of 200-800 mW / cm2; an ultrasonic generator for generating ultrasonic waves according to the control state of the main control unit; an ultrasonic vibrator that vibrates according to ultrasonic waves inputted from the ultrasonic generator; and a probe for transferring ultrasonic vibration to a joint to be treated. The ultrasonic device can induce the regeneration of cartilage tissue by the activation of cartilage tissue, and thus, can fundamentally treat degenerative arthritis.

The invention relates to a deer bone collagen soft capsule and a preparation method thereof. Bone collagen is also named as structural protein, accounts for 30 to 40 percent of the total amount of human protein, and is distributed in tendons for connecting human muscles, cartilaginous tissues and connective tissues for connecting joints and a dermis layer of skin. The preparation method of the deer bone collagen soft capsule comprises the following steps of: selecting materials; crushing; stewing; separating; grinding; performing enzymolysis; performing secondary separation; freezing; drying; passing through a soft capsule production line to prepare the finished product; and performing sterilization and packaging. The method is used for preparing the deer bone collagen soft capsule.

The present invention relates to a composition for cartilaginous tissue repair and to a production method therefor. The present invention comprises the steps of: (a) dissolving freeze-dried fibrinogen in an aprotinin solution; (b) dissolving freeze-dried thrombin in a stabilizing solution; (c) mixing an enriched collagen solution with thrombin and the stabilizing solution; and installing the fibrinogen solution (a) to one side of a dual kit and the solution (c) containing the collagen to the other side, and then mixing and injecting into damaged cartilaginous tissue. In the present invention, which is constituted as described above, biomaterials such as collagen and fibrin are mixed so as to allow damaged cartilaginous tissue to be repaired to a state allowing transplantation onto the tissue, and efficient regeneration is induced, thereby making it possible to reduce surgery-related stress on people and animals while inducing relatively rapid and efficient cartilage repair and regeneration.