117 results about "Cartilaginous Tissue" patented technology

The present application relates to chondrocyte compositions and kits comprising morselized cartilage tissue particles and a biodegradable matrix. Applications and methods of using the chondrocyte composition for cartilage grafts, repairing cartilage injuries and defects, and joint repair are provided.

The invention relates to the technical field of biology, in particular to a method for preparing a high-activity small-molecule cartilage extract from animal cartilage tissue, the cartilage extract and application of the cartilage extract, and the method for preparing the high-activity small-molecule cartilage extract from the animal cartilage tissue comprises the following steps: S1, soaking; s2, bone breaking; s3, removing cartilage fat by adopting a skimming and centrifugal separation process; s4, enzymolysis: performing compound shearing on compound protease to obtain enzymatic hydrolysate; s5, filtering; and S6, freeze drying. Through the application of various new technologies, the content of type II collagen in the prepared cartilage extract reaches 65% or above, the content of GAGS reaches 30% or above, the content of hyaluronic acid reaches 17% or above, and the problem that in the prior art, biological activity is damaged due to the processes of degreasing through a chemical degreasing agent, physical high temperature, chemical degradation and high-temperature drying is solved; the biological activity of the natural extract is completely reserved by adopting a physical degreasing and multi-enzyme multi-level directional composite enzymolysis technology and a freeze-drying technology at the temperature of minus 40 DEG C or below.

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention relates to bionic bio-ink as well as a preparation method and application thereof, and relates to the technical field of 3D bio-printing. The preparation method of the bionic bio-ink comprises the following steps: sequentially adding methylacryloylated recombinant human collagen, o-nitrobenzyl modified methylacryloylated hyaluronic acid, elastin-like polypeptide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphonate into a solvent, stirring and uniformly mixing to obtain an ACEM hydrogel precursor solution, and carrying out ultrasonic treatment on the ACEM hydrogel precursor solution to obtain the bionic bio-ink. Uniformly mixing the ACEM hydrogel precursor solution with a photoresist and seed cells to obtain the bionic biological ink. The sequence of the elastin-like polypeptide is as shown in SEQ ID NO. 1. The biological ink is better in mechanical strength, retains the advantages and functions of natural protein, can be quickly photo-cured into a high-strength and dual-network hydrogel structure, and has excellent printability, biocompatibility and cartilage tissue regeneration promoting capacity.

The invention discloses composite gel for osteochondral repair as well as a preparation method and application of the composite gel. Main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized III-type collagen (rhCol-III), and auxiliary raw materials of the composite gel are sodium hyaluronate (HA), fibrinogen and thrombin. The preparation method comprises the following steps: firstly, mixing an RSF solution with an rhCol-III solution, obtaining RSF-rhCol particles through a freeze drying technology and mechanical pulverization, dispersing the particles in an HA solution to obtain composite gel, mixing the gel with a thrombin solution, catalyzing fibrinogen to be split and converted into fibrin by thrombin, and performing freeze drying to obtain the rhCol-RhCol-RhCol-RhCol-RhCol-RhCol-RhCol. And crosslinking the fibrous protein monomer to obtain the injectable composite gel preparation capable of immediately gelling. The prepared RSF-rhCol composite gel can adapt to a defect area, has a certain adhesion effect, has excellent biocompatibility, promotes migration of bone marrow mesenchymal stem cells, induces cell differentiation and promotes cartilage tissue regeneration.

The invention discloses a cartilage regeneration and repair method and system based on an irreversible electroporation technology. The method comprises the following steps: establishing an electroporation model of a cell membrane, simulating the distribution of an electric field and the potential change of the cell membrane, and predicting a membrane perforation effect; analyzing potential change according to an electroporation model, and combining energy consumption change of the cell membrane to obtain a critical condition of cell membrane pore formation; in combination with critical conditions, establishing an adaptive adjustment feedback mechanism, controlling the adjustment of electroporation parameters, and performing cartilage related gene transfection and cartilage repair; setting a comprehensive evaluation function, evaluating the repairing effect of the cartilage tissue, and adjusting electroporation parameters and a gene transfection strategy. The system comprises an electroporation model modeling module, a critical analysis module, an adaptive mechanism establishment module, a transfection repair module and an evaluation adjustment module. According to the method, the transfection efficiency of cartilage repair related genes can be improved and cartilage regeneration can be promoted by accurately regulating and controlling electroporation parameters and combining a self-adaptive feedback mechanism.

The present invention relates to the field of biomedical materials, and in particular to a method for preparing a decellularized cartilage matrix material derived from pig rib cartilage, comprising the following steps: (1) freezing and thawing the pig rib cartilage for 5 times; (2) soaking the pig rib cartilage frozen and thawed in step (1) in a sodium borohydride solution; (3) soaking the pig rib cartilage treated with sodium borohydride in step (2) in a sodium dodecyl sulfate solution; and (4) enzymolyzing the pig rib cartilage obtained in step (3) with a mixed solution of DNase and RNase. The present invention is suitable for decellularization of cartilage tissue with a thickness of 300 microns, and utilizes the spontaneous generation of hydrogen by the sodium borohydride solution to effectively expand the cartilage tissue, and then the surfactant SDS fully destroys the cell membrane, effectively removes the genetic material, and retains the effective components or extracellular matrix of the natural cartilage to the greatest extent.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention discloses an application method for revealing RIPK3 as a pathological index of osteoarthritis based on transcriptomics analysis, and belongs to the technical field of molecular biology and genetic engineering.The method comprises the following steps that S1, a Trizol reagent is used for conducting total RNA extraction on an articular cartilage specimen, and the RIN number gt is selected; 7.0, constructing a sequencing library by using the high-quality RNA sample; s2, carrying out quality inspection on the constructed library, and carrying out mRNA sequencing by using an Illumina NovaseqTM6000 sequencer after all the libraries are qualified in quality inspection; s3, carrying out bioinformatics analysis on data generated by high-throughput sequencing; s4, different parts of the cartilage tissue of the OA patient are labeled with RIPK3 through immunofluorescence, it is proved that expression of the RIPK3 in cartilage cells has strong correlation with the pathology of the OA focus, and the feasibility of taking the RIPK3 as a clinical pathology index is proved.

The present invention discloses a recombinant collagen and its use in cartilage repair matrix. The recombinant collagen provided by the present invention comprises a sequence shown in SEQ ID No.1; the amino acid sequence of the recombinant collagen comprises N basic repeating units, and the basic repeating unit contains n1 of the following characteristic amino acid sequences: "G‑Xaa1‑Xaa2‑G‑E‑Xaa3"; the 3' end and the 5' end of the basic repeating unit are connected to form the above-mentioned characteristic amino acid sequence. The recombinant collagen provided by the present invention has a relatively obvious integrin binding activity, has the effects of promoting cell adhesion, proliferation, differentiation, repairing cartilage tissue defects, etc., and has good application prospects.

The invention belongs to the technical field of pet bone joint prevention and treatment, and particularly provides a stem cell exosome and application thereof in preparation of medicine for preventing and treating pet bone joint diseases. The RANKL monoclonal antibody 5F16 and the mesenchymal stem cell exosome are combined for the first time to be used for preventing and treating the bone joint diseases of the pets, and test analysis shows that the preparation can effectively reduce the levels of IL-1beta, TNF-alpha and IL-6 in serum of the pets caused by bone joint injuries, inhibit secretion of the IL-1beta, the TNF-alpha and the IL-6, repair the bone joint injuries, improve and promote cartilage tissue repair, prevent and treat the bone joint diseases of the pets and prevent and treat the bone joint diseases of the pets. Good application prospects are realized.

The utility model relates to a thighbone side guide plate based on two-point bridging type knee joint unicompartmental knee arthroplasty, which comprises a first auxiliary plate, a second auxiliary plate and a third auxiliary plate, modeling is carried out through knee joint CT data before an operation, and two cartilage-free bone surfaces around a thighbone operation area are set as supporting points, so that the thighbone side guide plate can be used for knee joint unicompartmental knee arthroplasty. A 3D printing technology is used for printing a guide plate used for thighbone side and tibia side positioning and osteotomy, the guide plate is provided with a first auxiliary plate, a second auxiliary plate and a third auxiliary plate, the second auxiliary plate and the third auxiliary plate can be directly attached to the bone surface of cartilage-free tissue, the position of the first auxiliary plate above an operation area is achieved through two-point positioning, and the position of the second auxiliary plate above the operation area is achieved through two-point positioning. The first auxiliary plate does not need to be attached to the bone surface of the operation area, that is, the first auxiliary plate is not affected by cartilage tissue above the operation area, the precision error caused by the cartilage tissue of the osteotomy area is avoided, an operator can be guided more rapidly and accurately to conduct positioning and osteotomy in the operation, and the operation time and process are shortened.

The invention provides a biological liquid medicine for promoting cartilage tissue regeneration by being injected into knee joints. The biological liquid medicine comprises the following components: 3%-4% of modified xanthan gum, 1.5%-2.5% of chitosan lactate wrapped by lipidosome, 0.2%-0.6% of strontium bromide, 1%-2% of disodium hydrogen phosphate, 1%-2% of sodium dihydrogen phosphate and the balance of water for injection to 100%, the modified xanthan gum is carboxymethylated xanthan gum. According to the biological liquid medicine, modified xanthan gum, chitosan lactate wrapped by lipidosome and strontium bromide are added, long-acting retention of the modified xanthan gum provides a stable release environment for the lipidosome and the strontium bromide, the lipidosome accurately delivers the chitosan lactate to an injured part, and the lipidosome and the strontium bromide act on ions to strengthen cartilage cell proliferation and matrix secretion, so that the cartilage cell proliferation and matrix secretion are enhanced; the three components are combined to improve the cartilage tissue damage repair degree of the knee joint and improve the cartilage tissue regeneration effect.

The invention discloses a parasitic loranthus exosome-like nano vesicle and application thereof in preparation of a medicine for treating temporomandibular joint osteoarthritis. The taxillus sutchuenensis exosome-like nano-vesicles are prepared by combining a differential ultracentrifugation method with an ultrafiltration method. Researches show that the taxillus sutchuenensis exosome-like nano-vesicles can treat temporomandibular joint osteoarthritis through the following aspects: 1) an anti-inflammatory function: effectively reducing expression of inflammatory factors; 2) a cartilage degeneration inhibition function: blocking a cartilage matrix degradation pathway, and slowing down cartilage tissue damage; the taxillus sutchuenensis exosome-like nano-vesicles have the advantages that the taxillus sutchuenensis exosome-like nano-vesicles have obvious protection and repair effects on relieving temporal-mandibular joint osteoarthritis related bone and cartilage damage, and accordingly the taxillus sutchuenensis exosome-like nano-vesicles have good application prospects.

The invention provides a mesenchymal stem cell medicine for promoting knee joint cartilage regeneration as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: reacting polyethyleneimine with linoleoyl chloride to prepare linoleoyl chloride modified polyethyleneimine for preparing curcumin lipidosome, and reacting with multifunctional peptide to prepare multifunctional lipidosome; the mesenchymal stem cells are mixed with self-assembled RADA16 polypeptide embedded with TGF-beta1 and BMP-6 and subcultured mesenchymal stem cells, the mixture is added into autologous platelet-enriched plasma, and the mesenchymal stem cell medicine for promoting knee joint cartilage regeneration is prepared. According to the invention, curcumin is embedded by lipidosome, mesenchymal stem cells can promote osteogenesis differentiation by using provided nutrients, and interference of inflammatory microenvironment is effectively prevented. Meanwhile, the self-assembled RADA16 polypeptide wraps TGF-beta1 and BMP-6, the synergistic effect is beneficial to regeneration and repair of cartilage tissue, proliferation of bone cells is promoted, and cartilage repair and regeneration are facilitated.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

This invention relates to a method for generating, repairing and / or maintaining connective tissue in a subject. In one embodiment, the invention relates to a method for generating, repairing and / or maintaining cartilage tissue in a subject. The present invention also relates to a method of treating and / or preventing a disease in a subject arising from degradation and inflammation of connective tissue.

The invention relates to the field of image processing and biomechanics, in particular to a wrist joint mechanical detection method based on finite element analysis, which comprises the steps of acquiring medical image data, preprocessing images, endowing material attributes, setting mechanical loads and applying boundary conditions, solving finite elements and analyzing results. Through finite element analysis, the mechanical behaviors of the wrist joint under different loads can be accurately simulated, and the stress and strain distribution conditions of the wrist joint can be comprehensively analyzed. According to the method, the stress condition of the bone can be detected, the mechanical response of the cartilage tissue can be analyzed in detail, richer and more accurate information is provided for clinical diagnosis, and independent segmentation and assignment processing can be carried out on the lesion part. According to the method, the influence of lesion tissues on the mechanical characteristics of the wrist joint is fully considered, personalized analysis is carried out on patients, a more accurate treatment scheme can be formulated for each patient, and the treatment effect is improved.

This application belongs to the technical field of stem cell therapy, and discloses a composition for treating degenerative arthritis and its application. The composition contains adipose stem cells in a mass fraction of 20-60%, bone marrow mesenchymal stem cells in a mass fraction of 10-30%, and traditional Chinese medicine in a mass fraction of 10-50%. By improving the culture medium of adipose stem cells, this application obtains adipose stem cells with stronger proliferation ability, and its miRNA-140 is highly expressed. After being compounded with bone marrow mesenchymal stem cells and turmeric powder in proportion, it can effectively inhibit inflammatory factors and accelerate the recovery of cartilage tissue.

The invention provides inter alia a method for enzymatic digestion of an extracellular matrix protein in a tissue sample, comprising the steps of:—providing an aqueous medium that comprises (i) a matrix metalloproteinase and (ii) a cation that is Ca2+ at a concentration of at least 2 mmol / L;—contacting said aqueous medium with a tissue sample that comprises an extracellular matrix protein under conditions that allow for enzymatic digestion of said extracellular matrix protein; wherein said tissue sample is a cartilage tissue sample.

The present application relates to the technical field of cell and tissue engineering, and particularly relates to a culture medium and method for preparing hypertrophic cartilage tissue. The hypertrophic cartilage induction culture medium can effectively improve the induction and differentiation efficiency of hypertrophic cartilage cells or tissue, and the prepared hypertrophic cartilage tissue has high biocompatibility and activity and performs excellently in promoting bone defect repair. The culture medium group for inducing iPSC differentiation to prepare hypertrophic cartilage cell membrane pieces can accurately and efficiently induce iPSC to differentiate into hypertrophic cartilage cells, and can further form hypertrophic cartilage cell membrane pieces; the hypertrophic cartilage cell membrane pieces can quickly induce the regeneration of osteogenic centers, and provide a solution for treating large-area bone defects, and have good application prospect.

The purpose of the present invention is to provide a cartilage tissue body that has a sufficient mechanical strength and thickness, that does not contain scaffold components derived from other animals, and that is highly safe. The present invention provides a method for producing a scaffold-free three-dimensional cartilage tissue, the method comprising a step for culturing a modularized polyimide porous membrane on which chondrocytes are seeded while allowing the membrane to float in a culture medium in a state in which the membrane is not fixed in a cell culture vessel for floating culture, while having shear stress applied to the modularized polyimide porous membrane. The present invention also provides: a scaffold-free three-dimensional cartilage tissue body; and a modularized polyimide porous membrane for producing a scaffold-free three-dimensional cartilage tissue body.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The application provides application of lactate in preparation of a medicine for promoting growth and repair of bone / cartilage tissue, and the medicine has the effect of any one of 1) to 2): 1) promoting growth of bone / cartilage tissue; and 2) promoting repair and healing of bone / cartilage tissue. The lactate includes one or more of magnesium lactate, sodium lactate, potassium lactate, ferrous lactate, chromium lactate, copper lactate, manganese lactate, zinc lactate, lithium lactate, aluminum lactate, chitosan lactate, halofuginone lactate, trimethoprim lactate, 1-ethyl-3-methyl imidazole L-(+)-lactate, 2-hydroxyethyl-trimethyl ammonium L-(+)-lactate and L-lactic acid-tetrabutyl ammonium salt. The chitosan lactate and the like can promote growth and repair of bone / cartilage tissue for the first time, and a new way is provided for growth and repair of bone / cartilage tissue.

The invention belongs to the technical field of stem cell treatment, and discloses a composition for treating degenerative arthritis and application of the composition, and the composition comprises the following components in percentage by mass: 20-60% of adipose-derived stem cells, 10-30% of bone marrow mesenchymal stem cells and 10-50% of traditional Chinese medicines. According to the application, the adipose-derived stem cells with stronger multiplication capacity are obtained by improving a culture medium of the adipose-derived stem cells, miRNA-140 of the adipose-derived stem cells is highly expressed, inflammatory factors can be effectively inhibited after the adipose-derived stem cells are compounded with bone marrow mesenchymal stem cells and turmeric powder in proportion, and the recovery of cartilage tissues is accelerated.

This invention provides methods for optimal implantation of a solid substrate for promoting cell or tissue growth or restored function in an osteochondral, bone or cartilage tissue in a subject in need thereof. The methods include selecting and preparing a solid substrate for promoting cell or tissue growth or restored function for implantation, which solid substrate has a length and width or that promotes a tight fit within the boundaries of the implantation site and is further characterized by a height sufficient such that when a first terminus of said solid substrate is implanted within a bone in a site for implantation, a second terminus of said solid substrate is at a height at least 2 mm less than an articular cartilage layer surface or is proximal to a tide mark region in said implantation site and optionally applying a biocompatible polymer layer to an apical surface of said implant, which layer does not exceed the articular cartilage layer surface in height. Tools for implementation of optimal positioning are described including a unique tool (5-120) for trimming cartilage at the implantation site.

Provided in the present invention is the use of lactate in the preparation of a drug for promoting the growth and repair of bone / cartilage tissue. The drug has the following effect of either 1) or 2): 1) promoting the growth of bone / cartilage tissue; or 2) promoting the repair and healing of bone / cartilage tissue. The lactate comprises one or more of magnesium lactate, sodium lactate, potassium lactate, ferrous lactate, chromium lactate, copper lactate, manganese lactate, zinc lactate, lithium lactate, aluminum lactate, chitosan lactate, halofuginone lactate, trimethoprim lactate, 1-ethyl-3-methylimidazolium L-(+)-lactate, 2-hydroxyethyl-trimethylammonium L-(+)-lactate and L-lactic acid-tetrabutylammonium salt. It is found in the present invention for the first time that chitosan lactate, etc. can promote the growth and repair of bone / cartilage tissue, thereby providing a new way to grow and repair bone / cartilage tissue.

The present invention relates to a blunt-tipped cartilage filling device and a surgical vehicle with the device. The device comprises a hard hollow rod, a blunt-tipped scraper and a handle. A hollow channel is arranged at the center of the hard hollow rod. The blunt-tipped scraper is fixedly arranged at the first end of the hard hollow rod; the blunt-tipped scraper comprises a blunt-tipped flattening disk and a through hole arranged at the center of the blunt-tipped flattening disk; the handle is sleeved and fixed on the hard hollow rod, and the second end of the hard hollow rod passes through the handle; an inlet is opened axially at the second end, and the inlet is used to communicate with an injection device, so that the separated and collected cartilage tissue of the patient himself or the cartilage tissue cultured in a laboratory is directly filled into the cartilage injury site of the patient through the inlet at the second end of the hard hollow rod, the hollow channel of the hard hollow rod and the through hole at the center of the blunt-tipped flattening disk of the blunt-tipped scraper in sequence; the blunt-tipped scraper is used to scrape and compact the cartilage tissue filled into the cartilage injury site of the patient.

The invention belongs to the technical field of biomedical materials, and particularly relates to collagen hydrogel loaded with gold nanoclusters and a preparation method and application thereof.The hydrogel is collagen wrapping the gold nanoclusters, the gold nanoclusters are connected with targeted polypeptide, and the targeted polypeptide is a collagen hydrogel loaded with the gold nanoclusters and a preparation method and application of the collagen hydrogel loaded with the gold nanoclusters. The hydrogel not only has a mutually communicated porous structure and relatively low swelling and enzymatic degradation resistance, but also has good mechanical strength and injectable performance; the hydrogel can intelligently respond to high-level active oxygen and regulate and control controllable release of the gold nanoclusters; the hydrogel has excellent biocompatibility and biological activity, and can remarkably promote cell proliferation and cartilage differentiation of bone marrow mesenchymal stem cells; the cartilage tissue is recognized in a targeted manner, the regeneration of the cartilage tissue is promoted, and the material can be applied to clinical treatment of osteoarthritis.

The invention discloses a multivalent triangular DNA origami delivery system as well as a preparation method and application thereof, which can be used as a novel method for treating osteoarthritis, and is particularly suitable for patients with cartilage degeneration and joint inflammation caused by age, obesity, joint reuse, sport injury or genetic factors and the like. Specific siRNAs (small interfering RNAs) are delivered by the use of multivalent triangular DNA origami. According to the scheme, the expression of inflammation and degeneration related genes, such as Stat3, MMP3 and MMP13, in cartilage cells in joints can be specifically inhibited, so that the inflammatory response is relieved, cartilage decomposition is inhibited, and repair and regeneration of cartilage tissues are promoted. The invention provides a method for treating osteoarthritis, which has the advantages of strong targeting property, small side effect and obvious curative effect, is suitable for clinical treatment and basic medical research, and has wide application prospect.