6 results about "Cartilaginous Tissue" patented technology

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

This invention discloses SFMBT2 The application of genes and bromocriptine in repairing damaged cartilage and preventing osteoarthritis. Experiments have found that the gene expressed in chondrocytes... SFMBT2 The gene helps maintain the normal phenotype of chondrocytes and can mediate the axial regulation of downstream ATF3-PI3K / AKT signaling. Cartilage tissue-specific knockout mice of this gene exhibit a significant spontaneous osteoarthritis phenotype and exacerbate the progression of DMM surgery-induced PTOA. Gene complementation targeting SMBT2 can significantly activate chondrocyte activity. Among various small molecule drugs targeting SMBT2 protein predicted using docking models, bromocriptine can significantly promote the repair of damaged cartilage by activating the biological function of chondrocytes.

The application provides a nucleic acid drug delivery carrier for efficiently targeting cartilage tissue, wherein a cationic lipid DOTAP is additionally added in a traditional four-component lipid nanoparticle to obtain a five-component lipid nanoparticle, and the five-component lipid nanoparticle is obtained by screening different ionizable lipids, phospholipid types, optimizing proportion relationships, particle sizes and electric potentials, and the five-component lipid nanoparticle has a hydration particle size less than 100 nm and a positive surface charge. In-vitro experiments show that the positive electric lipid nanoparticle has a transfection efficiency on chondrocytes obviously higher than that of a negative electric lipid nanoparticle, and can penetrate a whole layer of cartilage in a short time. Animal experiments show that the positive electric lipid nanoparticle has a significantly prolonged retention time in a joint cavity, a higher protein expression amount, and is mainly concentrated in cartilage tissue and almost not expressed in synovial membrane and other tissues. In a word, the application successfully realizes efficient targeting of cartilage tissue by using a simpler strategy, and has a simple method, uniform and stable quality, and can be produced in a large scale, and has an excellent conversion prospect.

FIELD: traumatology; orthopedics; physiotherapy.SUBSTANCE: used to treat post-traumatic osteoarthrosis of the knee joint after surgery. On the 15–17th day of the postoperative period, local cutaneous exposure to nitric oxide is carried out at a pressure of 450–500 ppm for 5–8 minutes on each affected joint. The course of treatment consists of 10-12 daily procedures and is repeated after 3–4 months.EFFECT: method increases the effectiveness of treatment, halts disease progression, and achieves cartilage tissue regeneration in damaged areas through the combined anti-inflammatory and stimulating effects of nitric oxide under strictly defined pressure and exposure parameters, which allows for the achievement of sustained regression of osteoarthritis stages and the prevention of relapses.1 cl, 4 ex

This invention discloses a method, targets, and applications for the diagnosis and treatment of hemophilic arthritis. The method includes: obtaining cartilage and synovial membrane samples from patients with hemophilic arthritis undergoing total knee arthroplasty; performing single-cell RNA sequencing, single-cell ATAC sequencing, and spatial transcriptomics analysis to determine the main cell types and key cell subpopulations constituting the microenvironment of hemophilic arthritis; identifying the molecular characteristics and differentiation processes of chondrocyte and synovial fibroblast subpopulations; measuring intercellular interactions in the knee joint microenvironment of the samples; and identifying specific regulatory transcription factors in hemophilic arthritis through paired epigenetic analysis to identify diagnostic and therapeutic targets for hemophilic arthritis. This method integrates single-cell multi-omics analysis to analyze the cellular characteristics of synovial and cartilage tissues and their interactions in the epigenetic pathogenic mechanism, identifying potential therapeutic targets.

This invention discloses a method for preparing dried cartilaginous fish skeleton specimens, aiming to address the common technical pain points in existing cartilaginous fish skeleton specimen preparation techniques, such as easy shrinkage and deformation of cartilage tissue, narrow applicable size range, lengthy production cycle, high chemical toxicity, or high operational difficulty. This method, through optimized process design, comprises five core steps: material processing, bleaching, dehydration, shaping, and specimen treatment. Combined with safe and non-toxic conventional materials, it successfully achieves standardized preparation of dried specimens of medium to large-sized complete cartilaginous fish skeletons with a snout length of over 20 cm. This invention not only significantly shortens the production cycle, but also uses safe, non-toxic, and environmentally friendly materials such as hydrogen peroxide, ethanol, and mixed desiccants, generating no harmful waste. Furthermore, it has a wide range of applications, efficiently preparing skeleton specimens of various cartilaginous fish such as rays and sharks, and can be extended to the preparation of fish ocular bones and cartilaginous tissue specimens of reptiles and mammals. It has extremely high practical value and broad prospects for promotion in fields such as biological teaching, scientific research display, and species resource preservation.