90 results about "Cartilaginous Tissue" patented technology

The present application relates to chondrocyte compositions and kits comprising morselized cartilage tissue particles and a biodegradable matrix. Applications and methods of using the chondrocyte composition for cartilage grafts, repairing cartilage injuries and defects, and joint repair are provided.

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention relates to bionic bio-ink as well as a preparation method and application thereof, and relates to the technical field of 3D bio-printing. The preparation method of the bionic bio-ink comprises the following steps: sequentially adding methylacryloylated recombinant human collagen, o-nitrobenzyl modified methylacryloylated hyaluronic acid, elastin-like polypeptide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphonate into a solvent, stirring and uniformly mixing to obtain an ACEM hydrogel precursor solution, and carrying out ultrasonic treatment on the ACEM hydrogel precursor solution to obtain the bionic bio-ink. Uniformly mixing the ACEM hydrogel precursor solution with a photoresist and seed cells to obtain the bionic biological ink. The sequence of the elastin-like polypeptide is as shown in SEQ ID NO. 1. The biological ink is better in mechanical strength, retains the advantages and functions of natural protein, can be quickly photo-cured into a high-strength and dual-network hydrogel structure, and has excellent printability, biocompatibility and cartilage tissue regeneration promoting capacity.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention belongs to the technical field of pet bone joint prevention and treatment, and particularly provides a stem cell exosome and application thereof in preparation of medicine for preventing and treating pet bone joint diseases. The RANKL monoclonal antibody 5F16 and the mesenchymal stem cell exosome are combined for the first time to be used for preventing and treating the bone joint diseases of the pets, and test analysis shows that the preparation can effectively reduce the levels of IL-1beta, TNF-alpha and IL-6 in serum of the pets caused by bone joint injuries, inhibit secretion of the IL-1beta, the TNF-alpha and the IL-6, repair the bone joint injuries, improve and promote cartilage tissue repair, prevent and treat the bone joint diseases of the pets and prevent and treat the bone joint diseases of the pets. Good application prospects are realized.

The utility model relates to a thighbone side guide plate based on two-point bridging type knee joint unicompartmental knee arthroplasty, which comprises a first auxiliary plate, a second auxiliary plate and a third auxiliary plate, modeling is carried out through knee joint CT data before an operation, and two cartilage-free bone surfaces around a thighbone operation area are set as supporting points, so that the thighbone side guide plate can be used for knee joint unicompartmental knee arthroplasty. A 3D printing technology is used for printing a guide plate used for thighbone side and tibia side positioning and osteotomy, the guide plate is provided with a first auxiliary plate, a second auxiliary plate and a third auxiliary plate, the second auxiliary plate and the third auxiliary plate can be directly attached to the bone surface of cartilage-free tissue, the position of the first auxiliary plate above an operation area is achieved through two-point positioning, and the position of the second auxiliary plate above the operation area is achieved through two-point positioning. The first auxiliary plate does not need to be attached to the bone surface of the operation area, that is, the first auxiliary plate is not affected by cartilage tissue above the operation area, the precision error caused by the cartilage tissue of the osteotomy area is avoided, an operator can be guided more rapidly and accurately to conduct positioning and osteotomy in the operation, and the operation time and process are shortened.

The invention provides a biological liquid medicine for promoting cartilage tissue regeneration by being injected into knee joints. The biological liquid medicine comprises the following components: 3%-4% of modified xanthan gum, 1.5%-2.5% of chitosan lactate wrapped by lipidosome, 0.2%-0.6% of strontium bromide, 1%-2% of disodium hydrogen phosphate, 1%-2% of sodium dihydrogen phosphate and the balance of water for injection to 100%, the modified xanthan gum is carboxymethylated xanthan gum. According to the biological liquid medicine, modified xanthan gum, chitosan lactate wrapped by lipidosome and strontium bromide are added, long-acting retention of the modified xanthan gum provides a stable release environment for the lipidosome and the strontium bromide, the lipidosome accurately delivers the chitosan lactate to an injured part, and the lipidosome and the strontium bromide act on ions to strengthen cartilage cell proliferation and matrix secretion, so that the cartilage cell proliferation and matrix secretion are enhanced; the three components are combined to improve the cartilage tissue damage repair degree of the knee joint and improve the cartilage tissue regeneration effect.

The invention discloses a parasitic loranthus exosome-like nano vesicle and application thereof in preparation of a medicine for treating temporomandibular joint osteoarthritis. The taxillus sutchuenensis exosome-like nano-vesicles are prepared by combining a differential ultracentrifugation method with an ultrafiltration method. Researches show that the taxillus sutchuenensis exosome-like nano-vesicles can treat temporomandibular joint osteoarthritis through the following aspects: 1) an anti-inflammatory function: effectively reducing expression of inflammatory factors; 2) a cartilage degeneration inhibition function: blocking a cartilage matrix degradation pathway, and slowing down cartilage tissue damage; the taxillus sutchuenensis exosome-like nano-vesicles have the advantages that the taxillus sutchuenensis exosome-like nano-vesicles have obvious protection and repair effects on relieving temporal-mandibular joint osteoarthritis related bone and cartilage damage, and accordingly the taxillus sutchuenensis exosome-like nano-vesicles have good application prospects.

The invention provides a mesenchymal stem cell medicine for promoting knee joint cartilage regeneration as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: reacting polyethyleneimine with linoleoyl chloride to prepare linoleoyl chloride modified polyethyleneimine for preparing curcumin lipidosome, and reacting with multifunctional peptide to prepare multifunctional lipidosome; the mesenchymal stem cells are mixed with self-assembled RADA16 polypeptide embedded with TGF-beta1 and BMP-6 and subcultured mesenchymal stem cells, the mixture is added into autologous platelet-enriched plasma, and the mesenchymal stem cell medicine for promoting knee joint cartilage regeneration is prepared. According to the invention, curcumin is embedded by lipidosome, mesenchymal stem cells can promote osteogenesis differentiation by using provided nutrients, and interference of inflammatory microenvironment is effectively prevented. Meanwhile, the self-assembled RADA16 polypeptide wraps TGF-beta1 and BMP-6, the synergistic effect is beneficial to regeneration and repair of cartilage tissue, proliferation of bone cells is promoted, and cartilage repair and regeneration are facilitated.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

The invention relates to the field of image processing and biomechanics, in particular to a wrist joint mechanical detection method based on finite element analysis, which comprises the steps of acquiring medical image data, preprocessing images, endowing material attributes, setting mechanical loads and applying boundary conditions, solving finite elements and analyzing results. Through finite element analysis, the mechanical behaviors of the wrist joint under different loads can be accurately simulated, and the stress and strain distribution conditions of the wrist joint can be comprehensively analyzed. According to the method, the stress condition of the bone can be detected, the mechanical response of the cartilage tissue can be analyzed in detail, richer and more accurate information is provided for clinical diagnosis, and independent segmentation and assignment processing can be carried out on the lesion part. According to the method, the influence of lesion tissues on the mechanical characteristics of the wrist joint is fully considered, personalized analysis is carried out on patients, a more accurate treatment scheme can be formulated for each patient, and the treatment effect is improved.

This application belongs to the technical field of stem cell therapy, and discloses a composition for treating degenerative arthritis and its application. The composition contains adipose stem cells in a mass fraction of 20-60%, bone marrow mesenchymal stem cells in a mass fraction of 10-30%, and traditional Chinese medicine in a mass fraction of 10-50%. By improving the culture medium of adipose stem cells, this application obtains adipose stem cells with stronger proliferation ability, and its miRNA-140 is highly expressed. After being compounded with bone marrow mesenchymal stem cells and turmeric powder in proportion, it can effectively inhibit inflammatory factors and accelerate the recovery of cartilage tissue.

The invention provides inter alia a method for enzymatic digestion of an extracellular matrix protein in a tissue sample, comprising the steps of:—providing an aqueous medium that comprises (i) a matrix metalloproteinase and (ii) a cation that is Ca2+ at a concentration of at least 2 mmol / L;—contacting said aqueous medium with a tissue sample that comprises an extracellular matrix protein under conditions that allow for enzymatic digestion of said extracellular matrix protein; wherein said tissue sample is a cartilage tissue sample.

The present application relates to the technical field of cell and tissue engineering, and particularly relates to a culture medium and method for preparing hypertrophic cartilage tissue. The hypertrophic cartilage induction culture medium can effectively improve the induction and differentiation efficiency of hypertrophic cartilage cells or tissue, and the prepared hypertrophic cartilage tissue has high biocompatibility and activity and performs excellently in promoting bone defect repair. The culture medium group for inducing iPSC differentiation to prepare hypertrophic cartilage cell membrane pieces can accurately and efficiently induce iPSC to differentiate into hypertrophic cartilage cells, and can further form hypertrophic cartilage cell membrane pieces; the hypertrophic cartilage cell membrane pieces can quickly induce the regeneration of osteogenic centers, and provide a solution for treating large-area bone defects, and have good application prospect.

The purpose of the present invention is to provide a cartilage tissue body that has a sufficient mechanical strength and thickness, that does not contain scaffold components derived from other animals, and that is highly safe. The present invention provides a method for producing a scaffold-free three-dimensional cartilage tissue, the method comprising a step for culturing a modularized polyimide porous membrane on which chondrocytes are seeded while allowing the membrane to float in a culture medium in a state in which the membrane is not fixed in a cell culture vessel for floating culture, while having shear stress applied to the modularized polyimide porous membrane. The present invention also provides: a scaffold-free three-dimensional cartilage tissue body; and a modularized polyimide porous membrane for producing a scaffold-free three-dimensional cartilage tissue body.

The present application discloses a bone cartilage repair scaffold and a preparation method thereof, wherein the bone cartilage repair scaffold includes a subchondral bone layer, a calcified cartilage layer and a cartilage layer connected in sequence, the calcified cartilage layer includes a calcified cartilage upper layer, a calcified cartilage middle layer, a calcified cartilage lower layer, and a porous gel embedded in the calcified cartilage upper layer and the calcified cartilage middle layer. In an embodiment of the present application, the above-mentioned bone cartilage repair scaffold and a preparation method thereof are adopted, and a layered structure is adopted to simulate human physiological cartilage. Through the layering and gradient porosity design of the calcified cartilage layer, not only the flexibility of the scaffold is improved and the performance of the scaffold is improved, but also it can ensure that the scaffold can quickly recruit the patient's autologous blood after being implanted into the bone cartilage defect site, thereby guiding bone marrow mesenchymal stem cells to differentiate into chondrocytes in the cartilage layer and promote the generation of cartilage tissue.

The application provides application of lactate in preparation of a medicine for promoting growth and repair of bone / cartilage tissue, and the medicine has the effect of any one of 1) to 2): 1) promoting growth of bone / cartilage tissue; and 2) promoting repair and healing of bone / cartilage tissue. The lactate includes one or more of magnesium lactate, sodium lactate, potassium lactate, ferrous lactate, chromium lactate, copper lactate, manganese lactate, zinc lactate, lithium lactate, aluminum lactate, chitosan lactate, halofuginone lactate, trimethoprim lactate, 1-ethyl-3-methyl imidazole L-(+)-lactate, 2-hydroxyethyl-trimethyl ammonium L-(+)-lactate and L-lactic acid-tetrabutyl ammonium salt. The chitosan lactate and the like can promote growth and repair of bone / cartilage tissue for the first time, and a new way is provided for growth and repair of bone / cartilage tissue.

This invention provides methods for optimal implantation of a solid substrate for promoting cell or tissue growth or restored function in an osteochondral, bone or cartilage tissue in a subject in need thereof. The methods include selecting and preparing a solid substrate for promoting cell or tissue growth or restored function for implantation, which solid substrate has a length and width or that promotes a tight fit within the boundaries of the implantation site and is further characterized by a height sufficient such that when a first terminus of said solid substrate is implanted within a bone in a site for implantation, a second terminus of said solid substrate is at a height at least 2 mm less than an articular cartilage layer surface or is proximal to a tide mark region in said implantation site and optionally applying a biocompatible polymer layer to an apical surface of said implant, which layer does not exceed the articular cartilage layer surface in height. Tools for implementation of optimal positioning are described including a unique tool (5-120) for trimming cartilage at the implantation site.

The invention discloses a multivalent triangular DNA origami delivery system as well as a preparation method and application thereof, which can be used as a novel method for treating osteoarthritis, and is particularly suitable for patients with cartilage degeneration and joint inflammation caused by age, obesity, joint reuse, sport injury or genetic factors and the like. Specific siRNAs (small interfering RNAs) are delivered by the use of multivalent triangular DNA origami. According to the scheme, the expression of inflammation and degeneration related genes, such as Stat3, MMP3 and MMP13, in cartilage cells in joints can be specifically inhibited, so that the inflammatory response is relieved, cartilage decomposition is inhibited, and repair and regeneration of cartilage tissues are promoted. The invention provides a method for treating osteoarthritis, which has the advantages of strong targeting property, small side effect and obvious curative effect, is suitable for clinical treatment and basic medical research, and has wide application prospect.

The invention discloses an osteochondral repair scaffold and a preparation method thereof.The osteochondral repair scaffold comprises a subchondral bone layer, a calcified cartilage layer and a cartilage layer which are sequentially connected, and the calcified cartilage layer comprises an upper calcified cartilage layer, a middle calcified cartilage layer and a lower calcified cartilage layer; according to the osteochondral repair scaffold and the preparation method thereof, the layered structure is adopted to simulate the physiological cartilage of the human body, and through the layering and gradient porosity design of the calcified cartilage layer, the flexibility of the scaffold is improved; the use performance of the scaffold is improved, and it can be ensured that the scaffold can rapidly recruit autoblood of a patient after being implanted into an osteochondral defect part, so that mesenchymal stem cells are guided to be differentiated into cartilage cells in a cartilage layer, and cartilage tissue generation is promoted.

The present invention relates to the field of medical device technology, and discloses an intelligent endplate grinding tissue fragment separation and collection device, comprising a grinding head and a handle; the grinding head comprises an outer sleeve, and a central control shaft is coaxially rotated in the outer sleeve; multiple sets of rotating arms are arranged along the radial direction of the central control shaft, and the other end of the rotating arm is hinged to a telescopic rod, the end of the telescopic rod passes through the outer sleeve and is connected to a double-edged blade, the double-edged blade is arranged tangentially along the outer sleeve, and the double-edged blade includes a hard bone blade and a cartilage blade arranged back to back. The central control shaft rotates so that one of the hard bone blade and the cartilage blade is tilted and the other is attached to the outer sleeve; a drive module, a power supply, and a controller are arranged in the handle, a first drive unit of the drive module is connected to the central control shaft, and a second drive unit is connected to a rotating drum at the bottom of the outer sleeve. In the present invention, the double-edged blade of the grinding head can achieve seamless connection between grinding cartilage tissue and grinding bony endplates, eliminating the need to change instruments and equipment back and forth during surgery, thereby improving surgical efficiency.

The application provides a method for improving the content of cartilage glycosaminoglycan by using cationic polymer, and the method promotes the content of glycosaminoglycan in cartilage tissue to be obviously increased by treating cartilage with a preparation containing polybrene, creates a good microenvironment for cartilage repair, and promotes the cartilage to be better repaired and regenerated; the method can improve the content of glycosaminoglycan in the differentiated tissue of mesenchymal stem cells by culturing the mesenchymal stem cells in an induction medium containing polybrene, promotes the mesenchymal stem cells to be better differentiated into cartilage, and has a wide clinical application prospect.

The present invention is to provide a 3D cartilage organoid block prepared by differentiating mesenchymal stem cells into 3D spheroid cartilage tissues, a basic unit for the 3D cartilage spheroid block. The inventors found that both the amount of GAG matrix and the expression of the collagen type2 increased. Therefore, the method of this invention provides clinically applicable cartilage tissues by effectively enhancing the function of the cartilage differentiation constructs according to 2D culture. The 3D cartilage organoid block can be usefully applied to the area, such as, articular cartilage regeneration and plastic surgery, where cartilage tissues restoration is required.

The present disclosure relates generally to joint health supplement compositions derived from a xenogeneic cartilaginous tissue source, methods of making such compositions, as well as methods of using such compositions, for example, to treat joint pain or other joint ailments due to arthritis in human and animal populations both at risk and actively presenting with such joint pain or other joint ailments. One aspect of the present disclosure can include a nutritional joint health supplement composition including a cartilaginous tissue material harvested from a xenogeneic tissue source and at least a trace amount of a detergent, wherein the nutritional joint health supplement composition has a beneficial component profile and reduced cellular and DNA content.

The present invention provides a simple, low-cost method for producing highly pure proteoglycan from a crushed cartilage tissue, a squeezed product of the crushed cartilage tissue, or an extract of the crushed or squeezed product. The crushed cartilage tissue, the squeezed product of the crushed cartilage tissue, or an extract of the crushed or squeezed product is immersed in or added to an aqueous solution containing salt, and alcohol is added to the resulting liquid composition in an amount to give a final concentration of 10 to 60% by volume to precipitate proteoglycans; alternatively, the crushed cartilage tissue, the squeezed product of the crushed cartilage tissue, or an extract of the crushed or squeezed product is immersed in or added to an aqueous solution containing salt and alcohol in an amount to give a final concentration of 10 to 60% by volume to precipitate proteoglycans.

This invention relates to a novel method for producing cartilage tissue in vitro, as well as methods for therapeutic uses and screening of the cartilage tissue produced therefrom.

The application discloses a SOD-containing patch for protecting bone joints and cartilage tissue and belongs to the fields of biological medicine and medical devices. The patch is in the form of a polymer microneedle, comprising a base layer and a needle tip layer; the needle tip layer comprises microneedles arranged in an array on the base layer, and the microneedles are loaded with SOD; and the base material of the microneedles is a high-molecular compound containing sodium hyaluronate and chondroitin sulfate. The patch has good anti-inflammatory, repairing and pain-relieving effects, all active ingredients in the patch are substances contained in the human body, the safety is extremely good, and the SOD is wrapped with sodium hyaluronate-chondroitin sulfate composite hydrogel, so that the SOD can be prevented from softening due to moisture absorption, thereby guaranteeing the treatment effect.

This invention discloses a composite gel for osteochondral repair, its preparation method, and its applications. The main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized type III collagen (rhCol-III), with sodium hyaluronate (HA), fibrinogen, and thrombin as auxiliary raw materials. In this invention, RSF solution and rhCol-III solution are first mixed, and RSF-rhCol microparticles are obtained through freeze-drying and mechanical pulverization. The microparticles are then dispersed in HA solution to obtain the composite gel. The gel is then mixed with thrombin solution, where thrombin catalyzes the cleavage of fibrinogen into fibrin. The fibrin monomers are then cross-linked to obtain an injectable composite gel formulation that can immediately gel. The RSF-rhCol composite gel prepared by this invention can adapt to defect areas and has a certain degree of adhesion. It also exhibits excellent biocompatibility, promotes the migration of bone marrow mesenchymal stem cells, induces cell differentiation, and promotes cartilage tissue regeneration.

The invention provides an osteoarthritis diagnostic marker screening method based on a proteomics technology, and relates to the technical field of medical treatment. The method comprises the following steps: acquiring a cartilage tissue sample and a normal cartilage tissue sample of a patient with osteoarthritis, performing proteomic detection on the samples by adopting a data independent acquisition technology to obtain protein expression data, screening out differential expression proteins from the protein expression data, and determining whether the differential expression proteins are different or not. And determining the function and pathway characteristics of the differential expression protein through bioinformatics analysis, and verifying the application of the differential expression protein as the diagnostic marker according to the function and pathway characteristics.

The present invention relates to the fields of image processing and biomechanics, and specifically is a wrist joint mechanical detection method based on finite element analysis, including: acquiring medical imaging data, image preprocessing, assigning material properties, setting mechanical loads and applying boundary conditions, finite element solution and result analysis. Through finite element analysis, the present invention can accurately simulate the mechanical behavior of the wrist joint under different loads, and comprehensively analyze its stress and strain distribution. The present invention can not only detect the stress conditions of the bones, but also conduct a detailed analysis of the mechanical response of the cartilage tissue, providing richer and more accurate information for clinical diagnosis, and can also perform separate segmentation and assignment processing for the lesions. The present invention fully considers the impact of the lesion tissue on the mechanical properties of the wrist joint, conducts personalized analysis for patients, and can formulate a more accurate treatment plan for each patient to improve the treatment effect.