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Cell-biodegradable material compound and its preparation method and application

A technology for degrading materials and complexes, applied in the field of cell-biodegradable material complexes and their preparation, can solve the problems of ethics and immune rejection restricting the application of embryonic stem cells, and achieves excellent effects, good histocompatibility, mechanical Excellent performance

Inactive Publication Date: 2008-03-26
南昌大学第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to their different sources, stem cells are divided into embryonic stem cells and adult stem cells. Although embryonic stem cells can differentiate into various cell types and form various tissues or organs of the body, ethical issues and risks such as immune rejection limit the application of embryonic stem cells.

Method used

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  • Cell-biodegradable material compound and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing a cell-biodegradable material composite, the method comprising the following steps:

[0028](1) Construction of PPOCP three-dimensional porous copolyester cell scaffolds, PPOCP with adjustable degradation behavior, excellent mechanical properties, and excellent biocompatibility, combined with salt porogen and normal temperature sub-mold to prepare stem cells suitable for planting The three-dimensional porous cell scaffold, the method comprising: using a grinding mill to pulverize sodium chloride particles, respectively passing through 60-mesh and 80-mesh stainless steel screens to prepare sodium chloride particles of 180-250 μm, and dissolving the PPOCP polymer in chloroform , add sodium chloride particles and stir evenly to make a suspension, pour it into a cylindrical container mold, control the porosity of the bracket to 90%, and the pore diameter to 180-250 μm, wait for the chloroform to volatilize for more than 12 hours in a ventilated place, ...

Embodiment 2

[0033] The composite cell carrier graft obtained in Example 1 for repairing tissue defects was transplanted in vivo to repair skin tissue damage: (1) New Zealand white rabbits were selected to replicate the full-thickness skin defect model, and the stem cells constructed in vitro were composited with the PPOCP carrier graft Transplanted in the skin defect area; (2) Observing the wound repair after operation, it can be seen that the surface of the graft is dry, and as time goes by, the white foamy graft gradually degrades, the wound and the graft are fresh and moist, and the graft completely forms new skin in about 4 weeks. However, the wound surface of the PPOCP material transplanted without stem cells shrinks significantly, and the growth of rabbit hair on the new skin is less, while the wound repaired with stem cells and PPOCP composites does not shrink significantly, and the appearance is basically close to normal skin. Frozen section HE staining The results showed that the ...

Embodiment 3

[0035] According to the needs of the tissue, the PPOCP material was molded into an oval capsule, and the composite cell carrier graft obtained in Example 1 for repairing tissue defects was transplanted in vivo to repair bladder tissue defects: (1) Experimental dogs were used to preserve the trigone of the bladder Subtotal cystectomy, implanted stem cells in vitro combined with PPOCP carrier grafts into the bladder defect area for bladder reconstruction; (2) Urodynamic observations were performed at 4, 8, 12, and 16 weeks after surgery, showing The bladder capacity repaired with stem cells and PPOCP composites is close to the preoperative normal level, and the bladder compliance is no different from that before operation, while the bladder capacity of PPOCP materials transplanted without stem cells is only half of the preoperative volume, which can only provide a small storage capacity. The water capacity and bladder compliance were only half of the preoperative normal level; X-...

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Abstract

The present invention relates to a cytobiological degradable material compound, its preparation method and application. Said compound is formed from polybutylene terephthalate / polyethylene glycol / digolactic acid (PBT / PEG / OLA) ternary copolyester (PPOCP) three-dimensional porous cell scaffold and stem cell, and the described stem cell is planted on the PPOCP three-dimensional porous cell scaffold. Said invention can be used for preparing composite cell carrier graft to repair injury of several tissue organs of skin, fat, urethra, bladder, soft bone tissue, muscular tissue and muscle tendon, etc.

Description

technical field [0001] The invention relates to medical tissue engineering, in particular to a cell-biodegradable material compound and its preparation method and application. Background technique [0002] The defect and / or loss of function of human tissues and organs caused by various reasons such as disease and trauma seriously endanger human health, and the repair of damaged tissues and organs has always been one of the difficult problems in the medical field. In recent years, with the development of cell biology and biomaterial science, tissue engineering has emerged as a new frontier subject in the high-tech field, which includes biology, materials science, engineering and medicine. Cross and merge. Tissue engineering refers to the application of the principles and technologies of engineering science and life science to develop biomaterial substitutes that can restore, maintain and improve the functions of damaged tissues and organs. The basic principle and method are ...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/26A61L27/60
Inventor 汪泱王共先陈义旺崔苏萍谢安朱祥军
Owner 南昌大学第一附属医院
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