70 results about "Cell scaffold" patented technology

The inventions provided herein relate to injection applicators, tissue markers and uses thereof, e.g., to mark a target tissue site (e.g., a biopsy site in a breast tissue) or to produce a cell scaffold. The tissue markers described herein are designed to be resistant to fast migration (e.g., immediate migration after implantation through a needle track) and slow migration (e.g., over an extended period of time) upon implantation at a target tissue site (e.g., a biopsy site in a breast tissue), without using an adhesive. Additionally or alternatively, the tissue markers described herein can be readily detectable by at least one imaging modality, e.g., but not limited to magnetic resonance imaging, X-ray imaging, ultrasound imaging, or a combination thereof.

The invention discloses a preparation method and application of a multi-material cell-loaded 3D scaffold, and the preparation method comprises the following steps: S1, preparing at least two kinds of printing ink; s2, various printing inks prepared in the step S1 are added into 3D printing equipment, and a 3D support is printed according to a designed model; the printing ink comprises a main material and a photoinitiator, the main material comprises one or a mixture of a photosensitive material and a non-photosensitive material, and at least one kind of printing ink further comprises a cell-loaded suspension. The processing and manufacturing efficiency and convenience can be improved, the manufacturing cost is reduced, meanwhile, dynamic culture and testing of the carrier cells are achieved, and the scientific research efficiency is improved.

Provided is a method for producing induced pluripotent stem cells by which induced pluripotent stem cells having high undifferentiated and differentiated capabilities can be easily obtained. This method for producing induced pluripotent stem cells comprises: a step for introducing a reprogramming factor into somatic cells; and a step for culturing the somatic cells into which the reprogramming factor is introduced in the presence of a cytoskeleton material containing a peptide-containing polyvinyl acetal resin.

The invention belongs to the technical field of biological material preparation, and particularly relates to a preparation method of a self-oxygen-supply hydrogel cell scaffold, an obtained product and application. According to the invention, stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres are realized. The method is realized by the following steps: adding compounds P-MA, P-Cys, ColMA and PEG-CaO2 into a PBS (Phosphate Buffer Solution), adding a photoinitiator, and irradiating and curing by ultraviolet light with the wavelength of 365nm to obtain the self-oxygen-supply composite hydrogel. The invention provides a novel method for applying composite hydrogel loaded with oxygen-producing nano-particles to 3D cell sphere culture and continuously releasing O2 in a slightly acidic environment to solve the problems of death of intermediate cells caused by oxygen deficit in the 3D culture process of existing cell spheres, indefinite components of matrigel, high price and the like. The problem of internal oxygen deficit in the cell ball culture process is solved, and the gel component is defined to be used for researching the effect between other components and cells. In addition, the self-oxygen-supply hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The invention provides 3D printing bio-ink as well as a preparation method and application thereof. The 3D printing bio-ink is prepared from the following components: methacrylic anhydride gelatin, oxidized hyaluronic acid, carboxymethyl chitosan and 2-methacryloyloxyethyl phosphorylcholine. By designing the specific composition of the 3D printing bio-ink, the 3D printing bio-ink which is good in printing performance, mechanical strength and electrical conductivity and high in cell survival rate after printing is prepared, and then a 3D printing stent with high cell-stent interaction is prepared and can be used for preparing a skin injury repair material.

Described are methods and devices for the generation of hydrogel particles with micrometer and submicrometer dimensions using oxygen-inhibited partial polymerization, and the particles generated therefrom. The described methods generate particles with dimensions independent of the starting polymerizable solution dimension, for example, a microdroplet. Further, microfluidic flow parameters (e.g. viscosity, flow rate) and photopolymerization process parameters (e.g. optical exposure intensity and duration) are controlled to generate particles with tunable crosslinking density-determined properties including elasticity, diffusivity, and biomolecular display for diverse applications such as drug delivery, tissue engineering cell scaffolds, and single- and multiple-cell therapeutics. Similarly, gradients of crosslinking density-determined properties can be created within single particles through the selection of optical exposure intensity and duration. In addition to conventional spherical shapes, a suite of non-spherical shapes may be generated by manipulating the dimensions of the microfluidic channels and other related physical and process parameters.

To provide a system, apparatus, device and method for an implantable analyte diffusion device.SOLUTION: Embodiments of the present disclosure include, for example, a medical device system including an implantable analyte diffusion device (ADID), the device comprising a cavity, a cell scaffold material (CSM) arranged within the cavity and configured to carry or otherwise retain a plurality of living cells adapted to secrete a therapeutic protein-based drug, and a porous hollow fiber membrane at least partially surrounding the cavity, wherein at least a portion of the membrane is adhered to the CSM by a high durometer adhesive that creates a hemispherical seal at both ends of the membrane. The system also includes a fill port having a dock means at a first end and a micro-diameter tube (MDT) extending from a second end. The MDT is sealed at a distal end to the fill port and at a proximal end to the cavity of the ADID to establish an integral cell fill pathway therein.SELECTED DRAWING: Figure 1

Provided is a cell scaffold material that improves the efficiency of introducing molecules into cells.　A cell scaffold material according to the present invention is for introducing molecules into cells, and contains a peptide-containing resin (A) having a synthetic resin part and a peptide part. The peptide part has a first peptide part and a second peptide part. The first peptide part and the second peptide part have different amino acid sequences. The first peptide part can bind to a first integrin, and the second peptide part can bind to a second integrin.

The invention relates to the field of cell culture, and provides a printing device and a printing system.The printing device comprises a feeding converter, a printing spray head and a printing bin, and the discharging end of the printing spray head extends into an inner cavity through an opening in the printing bin; and a butt-joint piece, used for inputting a printing material, of the feeding converter communicates with a feed port butt-joint piece of the corresponding printing spray head, so that a cell raw material and a hydrogel raw material are introduced, and a cell scaffold is printed into a culture dish in the printing bin. In the printing process, the discharging end and the culture dish are located in the printing bin, closed printing is achieved, and the infection risk can be effectively reduced. After printing is finished, the feeding converter corresponding to the feeding port can switch the butt joint piece, gas is introduced into the first channel and the second channel to discharge remaining cell raw materials and hydrogel raw materials, and printing materials are effectively cut off at the discharging end, so that manual cutting is omitted, pollution reduction is facilitated, meanwhile, manpower can be saved, and large-scale production is facilitated.

The present invention relates to the field of cell culture, and provides a printing device and a printing system. The printing device includes a feed converter, a print nozzle, and a print chamber. The discharge end of the print nozzle extends into the inner cavity through an opening on the print chamber. The docking piece of the feed converter used to input the printing material is connected to the corresponding feed port docking piece of the print nozzle, so that the cell raw material and the hydrogel raw material are introduced to print the cell scaffold in the culture dish in the print chamber. During the printing process, the discharge end and the culture dish are located in the print chamber to achieve closed printing, which can effectively reduce the risk of infection. After the printing is completed, the feed converter corresponding to the feed port can switch the docking piece, and gas is introduced into the first channel and the second channel to discharge the remaining cell raw material and hydrogel raw material, effectively cutting off the printing material at the discharge end, thereby eliminating the need for manual operation and cutting, which is beneficial to reducing pollution, while also saving manpower and facilitating large-scale production.

The invention provides a cell three-dimensional dynamic culture micro-fluidic chip which is suitable for cell three-dimensional culture, drug screening, tumor migration research and the like. The cell culture micro-device is composed of three layers including a cell sample injection layer, a cell scaffold layer and a culture solution sample injection layer. The device is characterized in that the cell sampling layer has a concave-convex structure; the culture solution sample injection layer simulates a human vascular structure and is provided with a permeable wall. Wherein the 3D printing cell growth bracket is embedded in a unit chamber of the culture solution sample injection layer and is assembled by a convex structure on the cell sample injection layer in an elastic interference fit manner, so that the bracket is prevented from being scoured and deformed by liquid. The invention has the advantages that: 1, the cells are uniformly injected and distributed in the cell scaffold; 2, a three-dimensional growth space is provided for cells; 3, nutrition is supplied to the cells, and cell metabolism waste liquid is discharged in time; 4, the bracket is stable in structure and can resist liquid scouring deformation; and 5, the micro-fluidic device can become a functional unit of an integrated micro-fluidic device and has wide applicability.

Provided is a coating solution for forming a cell scaffold with which a cell scaffold having a large thickness can be easily formed and the proliferation of cells can be enhanced. A coating solution for forming a cell scaffold according to the present invention contains a peptide-conjugated resin having a synthetic resin moiety and a peptide moiety and an alcohol solvent, a content of the peptide-conjugated resin being 0.1 wt% or more.

The present invention relates to a conditioning chamber (10) for conditioning tissue cells, comprising chamber walls (20) which surround a chamber volume (30) for receiving a cell scaffold (200), wherein at least one of the chamber walls (20) has a wave transfer portion (62) for receiving shock waves from a shock wave generator (140) and transferring the received shock waves to the chamber volume (30).

Provided is a production method for induced pluripotent stem cells by which induced pluripotent stem cells having favorable quality can be easily obtained in a short period of time. A production method for induced pluripotent stem cells according to the present invention includes, in the stated order: a seeding step of seeding somatic cells on a cell culture substrate including a cell scaffold; a removal step of removing suspended somatic cells without adhering to the cell scaffold; and an introducing step of introducing a reprogramming factor into somatic cells adhering to the cell scaffold.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

Provided is a coating solution for forming a cell scaffold with which a cell scaffold having a large thickness can be easily formed and the proliferation of cells can be enhanced. A coating solution for forming a cell scaffold according to the present invention contains a peptide-conjugated resin having a synthetic resin moiety and a peptide moiety and an alcohol solvent, a content of the peptide-conjugated resin being 0.1 wt % or more.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

A cell culture insert is described herein which is intended for use with a perfused cell culture. The insert comprises a generally cylindrical retaining ring for retaining the insert in a cell culture well. The retaining ring has upper and lower openings formed by a central bore, which has a stepped profile so that a narrower portion of the bore is spaced from the lower opening. The insert also comprises a cell scaffold, a filter and a compression ring. The compression ring is sized and shaped to be received within the lower opening of the retaining ring. The cell scaffold and filter are received within the lower opening of the retaining ring and the cell scaffold is exposed to the upper opening of the retaining ring. The compression ring retains the scaffold and filter within the retaining ring against the narrower portion of the bore. A cell culture system comprising the one or more inserts, and a multiwell cell culture plate are also described herein.

The invention belongs to the field of biomedical engineering materials, and provides a degradable piezoelectric / conductive cytoskeleton for nerve injury repair, which is prepared according to the following steps: immersing a crosslinked gelatin fiber membrane into excessive graphene oxide liquid, irradiating with ultraviolet light, stirring, and reducing with Na2S2O4. The gelatin fiber membrane with the surface coated with the graphene, which is obtained by the method, can be used for culturing neural stem cells, and the obtained fiber membrane attached with the neural stem cells can be used as a medical engineering material for neural restoration. The graphene and the gelatin fiber are tightly combined to form a continuous phase, so that the conductive performance is excellent, piezoelectric signals generated by the gelatin can be collected and transmitted by the graphene, and more remarkable electric output is generated. The cell scaffold can induce neural differentiation of stem cells in a lead-free mode that electric signals are generated through ultrasonic driving, the risk of infection of external leads is avoided, and the cell scaffold is safer and has remarkable clinical value; and the used materials are gradually degraded in vivo, so that long-term influence in vivo is avoided.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The present invention relates generally to nano-scale lines and nano-electronic devices, which in some aspects can be embedded in biological structures, such as tissues, organoids, organs, organisms, and the like. For example, one aspect generally relates to a stretchable cytoskeleton that can be connected to an electrical circuit. In some cases, a biological structure may be formed around the scaffold and deformed when formed, thereby causing the cell scaffold to be embedded in the biological structure. In certain embodiments, the scaffold may be connected to an external device, for example, to determine a property (e.g., an electrical property) of the cellular scaffold, and / or to apply a stimulus (e.g., an electrical stimulus) to the biological structure. Other aspects of the invention generally relate to methods of making or using such cell scaffolds, kits comprising such cell scaffolds, biological structures comprising such cell scaffolds, and the like.

The present application belongs to the technical field of biomaterial preparation, and particularly relates to a preparation method of a self-oxygen-supplying hydrogel cell scaffold, the obtained product and application. The present application realizes stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres. The method is realized through the following steps: compounds P-MA, P-Cys, ColMA and PEG-CaO2 are added to a PBS solution, a photoinitiator is added, and a'self-oxygen-supplying' composite hydrogel is obtained by irradiation with ultraviolet light of a wavelength of 365 nm. The present application provides a composite hydrogel loaded with oxygen-producing nanoparticles for 3D cell sphere culture and a new method for continuous release of O2 in a slightly acidic environment, aiming at the problems of intermediate cell death caused by hypoxia in the process of 3D culture of existing cell spheres, unclear matrigel composition, high price and the like. The present application solves the problem of internal hypoxia in the process of cell sphere culture, and clearly defines the gel composition for studying the interaction between other components and cells. In addition, the self-oxygen-supplying hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

Provided is a method for producing induced pluripotent stem cells with which it is possible to easily obtain induced pluripotent stem cells having good quality in a short time. A method for producing induced pluripotent stem cells according to the present invention comprises, in the following order: an inoculation step for inoculating somatic cells on a cell culture substrate provided with a cell scaffold material; a removal step for removing the suspended somatic cells that do not adhere to the cell scaffold material; and an introduction step for introducing a reprogramming factor into the somatic cells adhered to the cell scaffold material.

The invention belongs to the technical field of biology, and particularly relates to a cytoskeleton for promoting proliferation of corneal limbal stem cells. The raw materials of the cytoskeleton provided by the invention are chitosan and sodium hyaluronate, and the cytoskeleton has good biocompatibility and biological activity, can simulate the characteristics of an extracellular matrix, provides necessary physical and chemical support for stem cells, and does not cause immune response or cytotoxicity. The addition of the magnetic nanoparticles further enhances the performance of the cytoskeleton. Directional arrangement under the action of a magnetic field can provide structural support closer to an in-vivo microenvironment for cells, and adhesion and proliferation of the cells are promoted. The cell scaffold provided by the invention can provide a good growth microenvironment for the corneal limbus stem cells, and is beneficial to solving the problem that the corneal limbus stem cells are easy to lose activity and function in in-vitro culture.

The invention belongs to the technical field of tissue engineering scaffold in-vitro culture, and particularly relates to a dynamic culture system and culture method for acellular scaffold tissue. The dynamic culture system comprises: a housing in communication with an oxygen supply device; the culture dishes are arranged on the upper portion of the shell in a stacked mode, and two connectors are formed in the bottom wall of each culture dish; the perfusion system comprises an oxygenator and a peristaltic pump which are communicated in sequence, and a polytetrafluoroethylene hollow fiber pipe in the oxygenator and the peristaltic pump are respectively connected with one interface; the joint is positioned in the culture dish and is communicated with the interface connected with the oxygenator; the power supply is used for supplying power to the peristaltic pump; the controller is used for controlling the operation of the peristaltic pump. The dynamic culture system effectively supports three-dimensional tissue growth, and overcomes the defects of a traditional system. The device is compact in design, simple and convenient to operate and capable of maintaining complex tissue constructs, so that the device becomes a powerful tool for tissue engineering application and regenerative medicine research.

In one embodiment, the present invention provides a composition, wherein the composition is a porous scaffold, wherein the pores of the scaffold are from 1 to 500 microns, the composition comprising: a) a cross-linkable protein selected from the group consisting of collagen and gelatin; b) a cross-linker which induces cross-linking of the cross-linkable protein; and c) a liquid.

Systems, methods, and devices disclosed herein include three-dimensional bioprinting technologies which produce cell scaffolds with a high degree of complexity and precision. A bioprinting ink mixture can be formed with coated gold nanoparticles in an alginate-gelatin solution. Upon bioprinting a 3D scaffold microstructure from the bioink mixture, an imaging procedure is performed on the 3D scaffold microstructure using the coated gold nanoparticles as a contrasting agent. A microstructure characterization is determined from the imaging procedure, and a virtual 3D reconstruction of the microstructure characterization can be generated for presentation on a graphical user interface (GUI) of a computing device. These techniques can be used to determine whether the resultant internal core of the microstructure properly mimics the architecture of the native tissue extracellular matrix (ECM) present in-vivo by determining the degree of pore interconnectivity which can improve cell distribution, attachment, and growth in the 3D scaffold microstructure.

The invention relates to the technical field of laser processing, in particular to a method and device for processing a high-longitudinal-width-ratio microstructure through axial shaping laser. According to the method, the complex coding hologram is loaded on the spatial light modulator, so that the axial intensity distribution of the Bessel beam can be accurately regulated and controlled, and the cylindrical voxel with the adjustable height can be prepared through single exposure. The voxel height can be continuously and gradually changed only by switching holograms generating Bessel beams with different focal lengths, and meanwhile, the maximum transverse size is kept unchanged. According to the method, frequent axial mechanical scanning or power adjustment is not needed in the machining process, and the machining efficiency and the operation stability can be improved; the method is suitable for rapid processing of microstructures with high longitudinal-width ratios, such as microcolumns, microtubes, cytoskeletons and binary diffraction devices.

The present invention relates to a cell culture insert 10 comprising the following features: a generally cylindrical retaining ring 12 for retaining the insert in a cell culture well (42 Fig. 3) and having upper 16 and lower 18 openings formed by a central bore 14 with a stepped profile (20, 22, 24 Fig. 2) providing a narrower portion of said bore from the lower opening; a cell scaffold 30; a filter 32; and a compression ring 34 sized and shaped to be received within the lower opening of the retaining ring; wherein the cell scaffold and filter are received within the lower opening of the retaining ring, the cell scaffold being exposed to the upper opening of the retaining ring, and wherein the compression ring retains the scaffold and filter within the retaining ring against said narrower portion (20 Fig. 2) of the bore. In preferred embodiments, a screw thread (Fig. 2) is provided on the exterior portion of the retaining ring.

The present invention provides an oral soft tissue repair device and method based on a biological cell scaffold, relating to the field of biological oral technologies. The device includes a bionic adaptive biological cell scaffold, a multi-dimensional precision 3D printing system, and an intelligent implantation and regulation device. The bionic adaptive biological cell scaffold is made of a composite of collagen, hyaluronic acid, and a biodegradable poly(lactic-co-glycolic acid) copolymer. The bionic adaptive biological cell scaffold has a multi-layer spiral pore network, and the inner wall of the pore network is embedded with microfluidic channels. Chemical-sensitive microvalves are arranged in the microfluidic channels. By integrating the bionic adaptive biological cell scaffold, the multi-dimensional precision three-dimensional printing system, and the intelligent implantation and regulation device, and combining finite element analysis and the particle swarm optimization algorithm, the present invention can accurately simulate the chewing dynamics and saliva secretion characteristics of patients, effectively solving the problem of the lack of dynamic adaptability in the static design of traditional scaffolds.