27 results about "Cell scaffold" patented technology

To provide a system, apparatus, device and method for an implantable analyte diffusion device.SOLUTION: Embodiments of the present disclosure include, for example, a medical device system including an implantable analyte diffusion device (ADID), the device comprising a cavity, a cell scaffold material (CSM) arranged within the cavity and configured to carry or otherwise retain a plurality of living cells adapted to secrete a therapeutic protein-based drug, and a porous hollow fiber membrane at least partially surrounding the cavity, wherein at least a portion of the membrane is adhered to the CSM by a high durometer adhesive that creates a hemispherical seal at both ends of the membrane. The system also includes a fill port having a dock means at a first end and a micro-diameter tube (MDT) extending from a second end. The MDT is sealed at a distal end to the fill port and at a proximal end to the cavity of the ADID to establish an integral cell fill pathway therein.SELECTED DRAWING: Figure 1

Provided is a cell scaffold material that improves the efficiency of introducing molecules into cells.　A cell scaffold material according to the present invention is for introducing molecules into cells, and contains a peptide-containing resin (A) having a synthetic resin part and a peptide part. The peptide part has a first peptide part and a second peptide part. The first peptide part and the second peptide part have different amino acid sequences. The first peptide part can bind to a first integrin, and the second peptide part can bind to a second integrin.

Provided is a coating solution for forming a cell scaffold with which a cell scaffold having a large thickness can be easily formed and the proliferation of cells can be enhanced. A coating solution for forming a cell scaffold according to the present invention contains a peptide-conjugated resin having a synthetic resin moiety and a peptide moiety and an alcohol solvent, a content of the peptide-conjugated resin being 0.1 wt% or more.

Provided is a production method for induced pluripotent stem cells by which induced pluripotent stem cells having favorable quality can be easily obtained in a short period of time. A production method for induced pluripotent stem cells according to the present invention includes, in the stated order: a seeding step of seeding somatic cells on a cell culture substrate including a cell scaffold; a removal step of removing suspended somatic cells without adhering to the cell scaffold; and an introducing step of introducing a reprogramming factor into somatic cells adhering to the cell scaffold.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The invention relates to the technical field of laser processing, in particular to a method and device for processing a high-longitudinal-width-ratio microstructure through axial shaping laser. According to the method, the complex coding hologram is loaded on the spatial light modulator, so that the axial intensity distribution of the Bessel beam can be accurately regulated and controlled, and the cylindrical voxel with the adjustable height can be prepared through single exposure. The voxel height can be continuously and gradually changed only by switching holograms generating Bessel beams with different focal lengths, and meanwhile, the maximum transverse size is kept unchanged. According to the method, frequent axial mechanical scanning or power adjustment is not needed in the machining process, and the machining efficiency and the operation stability can be improved; the method is suitable for rapid processing of microstructures with high longitudinal-width ratios, such as microcolumns, microtubes, cytoskeletons and binary diffraction devices.

The application discloses a three-dimensional cell scaffold based on a leaf vein of nandina domestica and a preparation method and application thereof, relates to the technical field of biomedical materials and tissue engineering, and discloses the following steps: taking the leaf vein of nandina domestica as a base material, and preparing the scaffold through alkali boiling, sodium periodate selective oxidation and amino-silica / chitosan composite modification. The scaffold retains the three-dimensional structure of the natural leaf vein completely, and significantly improves the cell compatibility of the material. Cell experiments prove that the scaffold has no cytotoxicity, can support the three-dimensional growth of mesenchymal stem cells and PC12 cells and the like with high survival rate, and effectively promotes cell proliferation. The method is simple, green, environmentally-friendly and low in cost, and the obtained scaffold has wide application prospects in the fields of tissue engineering, regenerative medicine and drug screening and the like.

A three-dimensional cell growth containment article is described, which includes a molded body channelized by removal of sacrificial channelizing element(s) therefrom, so that the molded body contains one or more channel(s) therein, with a matrix material in at least one of such channel(s) that is supportive of three-dimensional cell growth in the matrix material. A method for making such articles is also described, in which a molded body is formed with one or more sacrificial channelizing element(s) therein, following which the sacrificial channelizing element(s) are removed. The three-dimensional cell growth containment articles of the present disclosure may be utilized in any applications in which there exists a need to reproducibly generate three-dimensional cellular structures, e.g., islet transplantation for diabetes treatment, transplantation of hormone secreting cells, cellular scaffolds for wound healing, and generation of tissue engineering structures to regain structural usefulness for orthopedic applications.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The application discloses a starch-based edible scaffold and a preparation method and application thereof, and belongs to the technical field of cell scaffold materials. The edible starch is used as a base material, and a semi-interpenetrating network (Semi-IPN) hydrogel scaffold is constructed through heat-induced self-assembly, and the scaffold has good biocompatibility and edible safety. The preparation method can accurately control the elastic modulus and mechanical properties of the scaffold, so that the scaffold is suitable for providing tensile stress stimulation for fish myoblasts. Thanks to the above-mentioned characteristics, the scaffold can not only effectively support the adhesion and proliferation of fish myoblasts, but also significantly promote the directional differentiation of the fish myoblasts, and form a biomimetic muscle tissue. The application provides an innovative and performance-controllable cell scaffold solution for producing fish culture meat products which do not need to be separated from the scaffold and can be directly eaten.

To provide a gelling agent capable of forming a gel composition having biocompatibility, biodegradability and cell adhesiveness.SOLUTION: The gelling agent includes a first polymer which is a derivative of a first biopolymer, a second polymer which is at least one selected from the group consisting of a second biopolymer and a derivative thereof, and a photopolymerization initiator. Of the first polymer and the second polymer, only the first polymer has a ureidopyrimidinone group, and only one of the first polymer and the second polymer has an ethylenically unsaturated group.SELECTED DRAWING: Figure 1

Provided is a cell scaffold material that can bring about good maintenance of memory T cells and good T cell proliferation.　A cell scaffold material according to the present invention is used for culturing a cell population containing T cells and contains a peptide-containing resin (A) having a synthetic resin part and a peptide part. The peptide part has a first peptide part and a second peptide part. The first peptide part and the second peptide part have different amino acid sequences. The first peptide part can bind to a first integrin, and the second peptide part can bind to a second integrin. The dispersive component γd of the surface free energy of the cell scaffold material is at least 24.5 mJ / m2 and not more than 45.0 mJ / m2, and the dipole component γp of the surface free energy of the cell scaffold material is at least 1.0 mJ / m2 and not more than 20.0 mJ / m2.

The invention relates to bioinformatics technology and specifically to an analysis method of a spatial transcriptome-based 4D-printed stem cell scaffold for enhancing diabetic skin injury healing. Spatial transcriptome sequencing is performed on tissues from both the PBS group and the combined treatment group. Cell clustering and annotation identify main cell types, followed by visualization of their spatial distributions in both groups. The proportions of various cell types are statistically quantified and plotted. Cell subsets exhibiting significant differential gene expression are enriched and verified. Using a 4D-printed stem cell scaffold combined with a piRNA inhibitor and stem cell therapy, the method reveals spatial distribution and dynamic cellular changes during wound healing. It identifies precise cellular locations and gene expression patterns within tissues, providing critical insights into the biological processes by which biomaterials promote diabetic injury repair. This approach offers new directions for future drug development.

Provided is a method for producing hematopoietic progenitor cells with which it is possible to stably obtain hematopoietic progenitor cells from pluripotent stem cells and with which it is possible to improve the efficiency of inducing differentiation from pluripotent stem cells into hematopoietic progenitor cells. This method for producing hematopoietic progenitor cells is provided with: a cell mass formation step in which pluripotent stem cells are cultured using a cell culture substrate provided with a substrate and a cell scaffold material arranged in a dot shape on a first surface of the substrate, and cell mass is formed on the surface of the cell scaffold material; and a differentiation induction step for inducing the differentiation of the obtained cell mass into a cell population containing hematopoietic progenitor cells.

The invention discloses a microsphere based on methacrylated gelatin, a preparation method of the microsphere and application of the microsphere in bio-ink, the microsphere is prepared by taking methacrylated gelatin as a core base material and selectively compounding glycosaminoglycan-methacrylated gelatin and / or lipidosome through a microfluidic technology, the size of the obtained microsphere is uniform, and the size of the microsphere is uniform. The composite components can endow the microspheres with excellent self-adhesion characteristics; a microfluidic technology is further adopted, a base material and a composite component are used as a microsphere shell phase, a cell dispersion liquid is used as a microsphere core phase, the cell-carrying microspheres with a core-shell structure are prepared, and the cell-carrying microspheres can well maintain normal survival and growth of embedded cells. The methylacryloylated gelatin-based microspheres or cell-loaded methylacryloylated gelatin-based microspheres can be used as 3D printing ink in the fields of preparation of scaffold materials, cell-loaded scaffold materials, adaptive cell three-dimensional culture, cell ball culture, high-density cell culture and tissue engineering implant materials.

Hydrogels that comprise one or more polymers and one or more milk proteins, wherein the polymer(s) and the milk protein(s) form an interconnected polymeric network via conjugation. In some embodiments, molecules of the polymer(s) are chemically modified to promote conjugation within the hydrogel. In some embodiments, molecules of the milk protein(s) are chemically modified to promote conjugation within the hydrogel. In some embodiments, molecules of both the polymer(s) and the milk protein(s) are chemically modified to promote conjugation(s) within the hydrogel to create the interconnected polymeric network. Examples of conjugation include, but are not limited to physical chain entanglement, ionic bonding, covalent bonding, secondary bonding, and hydrophobic-hydrophobic interactions, among others. The hydrogels can be enhanced with one or more cell-adhesion agents. The hydrogels can be used to form microcarriers and / or cell scaffolds for holding and proliferating living cells for creating comestible products, such as cultivated meat products, among others.

A method for preparing pet food by using animal-derived muscle stem cells, and a product obtained therefrom. The method comprises an integrated separation and culture technique for highly active muscle and adipose progenitor cells, and a large-scale three-dimensional culture and differentiation technique combined with a cell scaffold. The method can significantly improve the efficiency of separating myogenic and adipogenic progenitor cells and reduce costs.

The application belongs to the field of biomedical materials, and particularly relates to a recombinant humanized type I collagen, a DLP 3D printing biological ink, a preparation method and application, an amino acid sequence of the recombinant humanized type I collagen is shown as SEQ ID NO. 1; the biological ink comprises 50-150 mg / mL methacrylated recombinant humanized type I collagen, 2.0-5.0 mg / mL photoinitiator and 0.1-0.5 mg / mL light absorber; the biological ink has excellent printability and biological activity, a printed recombinant type I collagen scaffold has excellent biological activity, can significantly promote cell migration, proliferation and differentiation; a double-layer cell-loaded scaffold simulating a natural skin structure can be prepared, and the double-layer cell-loaded scaffold shows the effects of promoting epidermal regeneration and dermal remodeling, reducing a wound ROS level, improving a local immune microenvironment and enhancing angiogenesis, and has a good clinical application prospect.

There is provided a cyclic peptide containing a cyclic segment containing an RGD sequence and having 8 to 14 amino acid residues. A thioether bond is formed between an amino acid residue Xa located on a most N-terminal side of the cyclic segment and an amino acid residue Xb located on a most C-terminal side of the cyclic segment. However, in a case where one of the amino acid residue Xa and the amino acid residue Xb is a cysteine residue, an α carbon of the other amino acid residue of the amino acid residue Xa and the amino acid residue Xb is separated from a sulfur atom of the cysteine residue by five or more atoms.

Provided is a production method for induced pluripotent stem cells in which induced pluripotent stem cells having high undifferentiation and differentiation potency are easily obtained. A production method for induced pluripotent stem cells according to the present invention includes the steps of: introducing an reprogramming factor into somatic cells; and culturing the somatic cells into which the reprogramming factor has been introduced in the presence of a cell scaffold containing a peptide-conjugated polyvinyl acetal resin.

The present invention generally relates to nanoscale wires and nanoelectronics, which in some aspects may be embedded in biological structures, such as tissues, organoids, organs, organisms, and the like. For example, one aspect is generally directed to stretchable cell scaffolds, which may be connected to electrical circuits. In some cases, a biological structure may form around the scaffold and deform the cell scaffold as it forms, thereby causing the cell scaffold to become embedded within the biological structure. The scaffold may be connectable in certain embodiments to an external device, e.g., to determine a property of the cell scaffold (e.g., an electrical property), and / or to apply a stimulus (e.g., an electrical stimulus) to the biological structure. Other aspects of the invention are generally directed to methods of making or using such cell scaffolds, kits including such cell scaffolds, biological structures containing such cell scaffolds, or the like.

The inventions provided herein relate to tissue markers and uses thereof, e.g., to mark a target tissue site (e.g., a biopsy site in a breast tissue) or to produce a cell scaffold. The tissue markers described herein are designed to be resistant to fast migration (e.g., immediate migration after implantation through a needle track) and slow migration (e.g., over an extended period of time) upon implantation at a target tissue site (e.g., a biopsy site in a breast tissue), without using an adhesive. Additionally or alternatively, the tissue markers described herein can be readily detectable by at least one imaging modality, e.g., but not limited to magnetic resonance imaging, X-ray imaging, ultrasound imaging, or a combination thereof.

There is provided a cyclic peptide having an amino acid sequence represented Formula (1). In the formula, Xa and Xb, and Xc and Xd each independently represent amino acid residues crosslinked through a thioether bond; X1 to X5 each independently represent an amino acid residue; R represents an arginine residue; G represents a glycine residue; D represents an aspartic acid residue; and m1 to m5 each independently represent an integer of 0 or more. However, the total number of amino acid residues represented by Xa, Xb, Xc, and Xd and represented by X1, X3, and X4 is 7 to 16.

The invention discloses a hydrogel microstructure preparation device and method. The device comprises a lifting type supporting assembly, a motor, a liquid storage device and a collecting pool. The motor is installed at the lifting end of the supporting assembly, the output end of the motor is connected with the liquid storage device, and the collecting pool is arranged below the liquid storage device; the liquid storage device comprises a liquid storage cylinder and one or more capillary tubes connected with the bottom of the liquid storage cylinder in an inclined mode. The device provided by the invention is simple in structure and convenient to use, can be used for preparing hydrogel microstructures with different sizes and different forms, and has the advantages of low cell damage, high structural uniformity, high preparation flux, high operation stability and the like when being used for 3D culture of cells and construction of cell scaffolds.

The invention relates to the technical field of biological detection, and provides a replaceable cell chip based on a bionic self-circulation microenvironment and an electrochemical in-situ monitoring method. The cell chip comprises a chip main body formed by curing polydimethylsiloxane, and a detection chamber and an electrode accommodating chamber are vertically distributed in the chip main body in a laminated manner; a detection electrode containing a cytoskeleton is arranged in the pluggable electrode plug assembly, and a guide groove design is adopted in the plugging process, so that stable contact and sealing performance of the electrode and a chamber interface are ensured; the double-liquid self-circulation micro-channel system is connected with the chip body through a liquid inlet and a liquid outlet, and a peristaltic pump is adopted to drive double-liquid circulation to realize synchronous cell culture and detection. The service life of the chip is remarkably prolonged through the replaceable design of the electrode plug, and the cell activity state and the drug response characteristic can be continuously monitored under the condition that the culture process does not need to be interrupted in combination with the real-time sensing function of the detection cavity and the dynamic microenvironment regulation and control of the double-liquid circulation system.

Disclosed herein are partially ordered polypeptides, which include a plurality of disordered domains and a plurality of structured domains. The partially ordered polypeptides may have phase transition behavior and form aggregates at, above, or below certain temperatures. Further provided are cellular scaffolds comprised of the partially ordered polypeptides.