174 results about "Cell scaffold" patented technology

The present invention relates to protein matrix materials and devices and the methods of making and using protein matrix materials and devices. More specifically the present invention relates to protein matrix materials and devices that may be utilized for various medical applications including, but not limited to, drug delivery devices for the controlled release of pharmacologically active agents, encapsulated or coated stent devices, vessels, tubular grafts, vascular grafts, wound healing devices including protein matrix suture material and meshes, skin / bone / tissue grafts, biocompatible electricity conducting matrices, clear protein matrices, protein matrix adhesion prevention barriers, cell scaffolding and other biocompatible protein matrix devices. Furthermore, the present invention relates to protein matrix materials and devices made by forming a film comprising one or more biodegradable protein materials, one or more biocompatible solvents and optionally one or more pharmacologically active agents. The film is then partially dried, rolled or otherwise shaped, and then compressed to form the desired protein matrix device.

The invention is directed to methods and compositions for preparing matrices for controlled delivery of at least one therapeutic or biological agent to a target site in a subject. This is accomplished using nanoparticles coupled to the therapeutic or biological agent that are incorporated within the matrix or reacted on the surface of the matrix.

The invention provides fibrin-based, biocompatible materials useful in promoting cell growth, wound healing, and tissue regeneration. These materials are provided as part of several cell and tissue scaffolding structures that provide particular application for use in wound-healing and tissue regenerating. Methods for preparing these compositions and using them are also disclosed as part of the invention. A variety of peptides may be used in conjunction with the practice of the invention, in particular, the peptide IKVAV, and variants thereof. Generally, the compositions may be described as comprising a protein network (e.g., fibrin) and a peptide having an amino acid sequence that comprises a transglutaminase substrate domain (e.g., a factor XIIIa substrate domain) and a bioactive factor (e.g., a peptide or protein, such as a polypeptide growth factor), the peptide being covalently bound to the protein network. Other applications of the technology include their use on implantable devices (e.g., vascular graphs), tissue and cell scaffolding. Other applications include use in surgical adhesive or sealant, as well as in peripheral nerve regeneration and angiogenesis.

A hydratable, highly absorbent keratin solid fiber or powder capable of absorbing a large weight excess of water may be produced by partially oxidizing hair keratin disulfide bonds to sulfonic acid residues and reacting the sulfonic acid residues with a cation. The neutralized suspension can be filtered, washed, and dried, leaving keratin solid which can be shredded into fibers and further ground into powder. Addition of water to the solid produces a hydrogel. The powder or hydrogel may be useful as an absorbent material, as a therapeutic for skin, or as an excipient. The keratin materials can be incorporated into nonwoven films. The hydrogel may be used as biocompatible viscoelastic filler for implant applications. Both the hydrogel and nonwoven materials are also suitable for use as tissue engineering scaffolds.

A cell-scaffold composition that comprised of at least five layers of biocompatible material, each of which layers is coated with a biological sealant material and contains different cells The cell-scaffold composition is located in a bioreactor that contains devices for mechanically stimulating each of the cells in each of the layers at distinct frequencies.

The present invention relates to protein biocoacervates and biomaterials and the methods of making and using protein biocoacervates and biomaterials. More specifically the present invention relates to protein biocoacervates and biomaterials that may be utilized for various medical applications including, but not limited to, drug delivery devices for the controlled release of pharmacologically active agents, coated medical devices (e.g. stents, valves . . . ), vessels, tubular grafts, vascular grafts, wound healing devices including protein suture biomaterials and biomeshes, dental plugs and implants, skin / bone / tissue grafts, tissue fillers, protein biomaterial adhesion prevention barriers, cell scaffolding and other biocompatible biocoacervate or biomaterial devices.

The invention is directed to methods and compositions for monitoring remodeling of an artificial tissue construct using image or contrast enhancing agents. The invention allows the growth, development, and remodeling of the artificial tissue to be monitored.

The invention is directed to methods and compositions for preparing matrices for controlled delivery of at least one therapeutic or biological agent to a target site in a subject. This is accomplished using nanoparticles coupled to the therapeutic or biological agent that are incorporated within the matrix or reacted on the surface of the matrix.

The present invention relates to protein biocoacervates and biomaterials and the methods of making and using protein biocoacervates and biomaterials. More specifically the present invention relates to protein biocoacervates and biomaterials that may be utilized for various medical applications including, but not limited to, drug delivery devices for the controlled release of pharmacologically active agents, coated medical devices (e.g. stents, valves . . . ), vessels, tubular grafts, vascular grafts, wound healing devices including protein suture biomaterials and biomeshes, dental plugs and implants, skin / bone / tissue grafts, tissue fillers, protein biomaterial adhesion prevention barriers, cell scaffolding and other biocompatible biocoacervate or biomaterial devices.

Apparatus and methods are described for generating autologous tissue grafts, the apparatus including a point of care SVF isolation unit that includes a tissue digestion chamber in fluid communication with a lipid separating chamber, whereby SVF cells are isolated without centrifugation; and a cell seeding chamber in fluid communication with the SVF isolation unit, said cell seeding chamber adapted to support a cell scaffold. Methods and materials for cell seeding, including through the provision of micro rough scaffold surfaces, are also provided.

The invention belongs to the technical field of biological materials and tissue repair and particularly relates to a multi-layer porous scaffold and a preparation method thereof. A suitable porous material is selected, after being clipped according to set size and requirements, the material is bonded by pore-forming adhesive which contains mixture of components of polymer / pore-forming particles / solvent, and pore-forming agent is removed after solidification and bonding, thus preparing the porous scaffold which has multi-layer structures communicated with each other. The scaffold has multi-layer structures, porous transition layers are among the layers of the scaffold, and the pores in an upper layer and a lower layer of the scaffold are communicated with each other; and the scaffold is suitable for transition of different cells and fusion of tissues in a process of tissue remodeling, and weakened separation between layers of the remolding tissue is avoided. The scaffold is suitable for a bionic three-dimensional cell scaffold which repairs a multi-layer tissue and other application fields.

A vascular stent carries living therapeutic cellular material. The stent in widely implantable over the vascular system, and allows either localized or systemic delivery of the therapeutic products produced by the cellular material to thereby enhance patient treatment.

Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and / or microplates.

The present invention discloses a colloid scaffolds for tissue engineering, comprising collagen and hyaluronic acid (HA). The collagen and the hyaluronic acid (HA) are mixed to form colloidal suspension. The ratio of the collagen and the hyaluronic acid is less than or equal to 300, and the best is less than or equal to 200. The colloidal scaffolds more comprises micro-fibers or nano-fibers which are used to enhance cell attachment and increase the strength of colloid scaffolds and reduce water loss and contraction of colloid scaffolds.

The invention relates to a cross-linked hyaluronic acid cell-scaffold material and its preparation method and application. The cross-linked hyaluronic acid cell-scaffold material is obtained by crosslinking of high-molecular hyaluronate and low-molecular hyaluronate. The proportion of hyaluronate disaccharide molecules which take part in the crosslinking is 0.5%-20%, and expansion rate of the hyaluronate disaccharide molecules in an isotonic solution is 80%-110%. The preparation method of the cell-scaffold material comprises two freeze drying steps. Hyaluronate mixed with a cross-linking agent is formed firstly; after a heating reaction, water is added for swelling to form gel; and freeze drying is then carried out to obtain the porous scaffold material. The scaffold has abundant pores, has a certain mechanical strength and pore size and has good hydroscopicity and biocompatibility. The scaffold can be used as a tissue engineering cell-scaffold in promoting cartilage injury repairing and also can be used for preparing a haemostasis anti-adhesion material.

The present invention concerns biologically-active cell-free scaffolds composed of extracts of cellular and / or extracellular compartments for use in tissue regeneration. The present invention also contemplates the novel concept of redesigning the biological scaffold by seeding cells thereon followed by cell elimination. Cells are seeded on the scaffold for a period of time during which a dynamic interaction occurs between the scaffold and the seeded cells, resulting in reshaping of the scaffold architecture and integration of newly synthesized matrix elements. Redesigning may improve the physical and biological characteristics of the scaffold, and also improve the matching of the scaffold to treat a specific target tissue or a specific patient, by seeding tissue-specific cells or by seeding cells which are autologous to a patient, respectively.

The present invention relates generally to a tissue preparation including tissue cells and extracts thereof useful for promoting or facilitating the growth, development and differentiation of cells and tissues. More particularly, the present invention provides muscle-derived material comprising intact or extracted extracellular matrix and / or cells as well as cytokines, growth factors and other components. The muscle preparations of the present invention resemble basement membrane and are derived from cellular-based material. The muscle preparation may be used in vitro or in vivo as inter alia, a cellular scaffold in various tissue engineering applications and in other cell culture systems for nurturing and enriching a range of cell types including, but not limited to, precursor and stem cells such as pre-adipogenic cells. The muscle preparation is also useful as a base for creams, such as in the cosmetic and topical therapeutic industries and as a matrix or additive in the food industry.

A cellular scaffold that is suitable for tissue regeneration, cell culture and in vitro assays. The invention relates to a layered cell scaffold that is seeded with mesenchymal and ectodermal cells. The layered cellular scaffold comprises an inoculum of mesenchymal cells and ectodermal cells positioned between two opposing scaffolds in a sandwich configuration. The layered cell scaffold provides a functional skin equivalent that is suitable for transplantation and in vitro cell-based assays.

The present invention provides a kind of bone tissue filling material. Spongy bone of ox is first treated to become inorganic matter, soaked in sodium pyrophosphate solution, dried and high temperature calcined to produce calcined bone tissue filling material. The filling material is soaked in suspension of zinc chloride, dried and high temperature calcined to obtain the zinc-containing calcined porous bone tissue filling material. The porous bone tissue filling material has low cost, good biocompatibility, homogeneous and communicated pores, certain mechanical strength, capacity of promoting bone growth and other features, and is suitable for filling bone deficiency, spinal column fusion and cell rack in bone tissue engineering, etc.

A device and method for achieving load-bearing living-tissue-to-living-tissue coupling comprises a myriad of fine fibers extending directly from within the substance of one tissue to within the substance of the other tissue. Fibers are similar in cross-sectional area to, or smaller than, host tissue cells. This enables fibers to provide a scaffolding into which proliferating cells of each tissue may grow to form a collagenous matrix enveloping individual fibers and transferring mechanical loads between each tissue's extracellular matrix and the fibers. Also taught are devices and methods (1) for delivering bundles of independent fibers into soft or hard tissue, (2) for transiently reducing tissue drag during insertion, (3) for temporarily stabilizing position during tissue ingrowth, and (4) for spatial distribution of fiber bending stress in the event of a hard tissue.

Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and / or microplates.

The invention provides fibrin-based, biocompatible materials useful in promoting cell growth, wound healing, and tissue regeneration. These materials are provided as part of several cell and tissue scaffolding structures that provide particular application for use in wound-healing and tissue regenerating. Methods for preparing these compositions and using them are also disclosed as part of the invention. A variety of peptides may be used in conjunction with the practice of the invention, in particular, the peptide IKVAV, and variants thereof. Generally, the compositions may be described as comprising a protein network (e.g., fibrin) and a peptide having an amino acid sequence that comprises a transglutaminase substrate domain (e.g., a factor XIIIa substrate domain) and a bioactive factor (e.g., a peptide or protein, such as a polypeptide growth factor), the peptide being covalently bound to the protein network. Other applications of the technology include their use on implantable devices (e.g., vascular graphs), tissue and cell scaffolding. Other applications include use in surgical adhesive or sealant, as well as in peripheral nerve regeneration and angiogenesis.

The invention provides a biological scaffold manufacturing method based on 3D printing and a biological scaffold. The method comprises the following steps: observing and analyzing different cell tissues; manufacturing a 3D structure capable of supporting a cell scaffold by adopting a water-soluble material; filling all inner pores of the 3D structure with a biodegradable material; and then, putting in a water solution for dissolving and removing the water-soluble material, thereby obtaining the biological scaffold composed of the biodegradable material. The pore diameters of the inner pores of the manufactured biological scaffold are different, thereby being favorable for growth of cells; and the biological scaffold manufacturing method based on 3D printing is simple and quick and is capable of meeting the manufacturing requirements.

The invention discloses an inducer and a method for inducing differentiation of adipose-derived stem cells to cartilage cells, belonging to the technical field of cell biology. According to the technical scheme, the inducer comprises TGF-Beta 3, FGF-18, dexamethasone, insulin, selenium, transferrin, linoleic acid, BSA, sodium pyruvate solution, L-ascorbic acid-2-phosphate and proline. The inducer and the method have the advantages that the synthesis and metabolism of the cartilage cells are rapidly promoted, the generation of cartilage is promoted, the expression of cartilage extracellular matrix II type collagen and glycosaminoglycan is remarkably improved, and the time required for in-vitro induction culture is reduced; and the inducer and the method are widely suitable for various culture systems, especially a pellet system and a three-dimensional cell scaffold culture system.

The present invention relates to a cytobiological degradable material compound, its preparation method and application. Said compound is formed from polybutylene terephthalate / polyethylene glycol / digolactic acid (PBT / PEG / OLA) ternary copolyester (PPOCP) three-dimensional porous cell scaffold and stem cell, and the described stem cell is planted on the PPOCP three-dimensional porous cell scaffold. Said invention can be used for preparing composite cell carrier graft to repair injury of several tissue organs of skin, fat, urethra, bladder, soft bone tissue, muscular tissue and muscle tendon, etc.

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g / L of EDTA (ethylene diamine tetraacetic acid), 10,000 U / L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g / L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

A cellular scaffold that is suitable for tissue regeneration, cell culture and in vitro assays. The invention relates to a layered cell scaffold that is seeded with mesenchymal and ectodermal cells. The layered cellular scaffold comprises an inoculum of mesenchymal cells and ectodermal cells positioned between two opposing scaffolds in a sandwich configuration. The layered cell scaffold provides a functional skin equivalent that is suitable for transplantation and in vitro cell-based assays.