39 results about "Cell scaffold" patented technology

The invention provides 3D printing bio-ink as well as a preparation method and application thereof. The 3D printing bio-ink is prepared from the following components: methacrylic anhydride gelatin, oxidized hyaluronic acid, carboxymethyl chitosan and 2-methacryloyloxyethyl phosphorylcholine. By designing the specific composition of the 3D printing bio-ink, the 3D printing bio-ink which is good in printing performance, mechanical strength and electrical conductivity and high in cell survival rate after printing is prepared, and then a 3D printing stent with high cell-stent interaction is prepared and can be used for preparing a skin injury repair material.

To provide a system, apparatus, device and method for an implantable analyte diffusion device.SOLUTION: Embodiments of the present disclosure include, for example, a medical device system including an implantable analyte diffusion device (ADID), the device comprising a cavity, a cell scaffold material (CSM) arranged within the cavity and configured to carry or otherwise retain a plurality of living cells adapted to secrete a therapeutic protein-based drug, and a porous hollow fiber membrane at least partially surrounding the cavity, wherein at least a portion of the membrane is adhered to the CSM by a high durometer adhesive that creates a hemispherical seal at both ends of the membrane. The system also includes a fill port having a dock means at a first end and a micro-diameter tube (MDT) extending from a second end. The MDT is sealed at a distal end to the fill port and at a proximal end to the cavity of the ADID to establish an integral cell fill pathway therein.SELECTED DRAWING: Figure 1

Provided is a cell scaffold material that improves the efficiency of introducing molecules into cells.　A cell scaffold material according to the present invention is for introducing molecules into cells, and contains a peptide-containing resin (A) having a synthetic resin part and a peptide part. The peptide part has a first peptide part and a second peptide part. The first peptide part and the second peptide part have different amino acid sequences. The first peptide part can bind to a first integrin, and the second peptide part can bind to a second integrin.

The invention provides a cell three-dimensional dynamic culture micro-fluidic chip which is suitable for cell three-dimensional culture, drug screening, tumor migration research and the like. The cell culture micro-device is composed of three layers including a cell sample injection layer, a cell scaffold layer and a culture solution sample injection layer. The device is characterized in that the cell sampling layer has a concave-convex structure; the culture solution sample injection layer simulates a human vascular structure and is provided with a permeable wall. Wherein the 3D printing cell growth bracket is embedded in a unit chamber of the culture solution sample injection layer and is assembled by a convex structure on the cell sample injection layer in an elastic interference fit manner, so that the bracket is prevented from being scoured and deformed by liquid. The invention has the advantages that: 1, the cells are uniformly injected and distributed in the cell scaffold; 2, a three-dimensional growth space is provided for cells; 3, nutrition is supplied to the cells, and cell metabolism waste liquid is discharged in time; 4, the bracket is stable in structure and can resist liquid scouring deformation; and 5, the micro-fluidic device can become a functional unit of an integrated micro-fluidic device and has wide applicability.

Provided is a coating solution for forming a cell scaffold with which a cell scaffold having a large thickness can be easily formed and the proliferation of cells can be enhanced. A coating solution for forming a cell scaffold according to the present invention contains a peptide-conjugated resin having a synthetic resin moiety and a peptide moiety and an alcohol solvent, a content of the peptide-conjugated resin being 0.1 wt% or more.

Provided is a production method for induced pluripotent stem cells by which induced pluripotent stem cells having favorable quality can be easily obtained in a short period of time. A production method for induced pluripotent stem cells according to the present invention includes, in the stated order: a seeding step of seeding somatic cells on a cell culture substrate including a cell scaffold; a removal step of removing suspended somatic cells without adhering to the cell scaffold; and an introducing step of introducing a reprogramming factor into somatic cells adhering to the cell scaffold.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

A cell culture insert is described herein which is intended for use with a perfused cell culture. The insert comprises a generally cylindrical retaining ring for retaining the insert in a cell culture well. The retaining ring has upper and lower openings formed by a central bore, which has a stepped profile so that a narrower portion of the bore is spaced from the lower opening. The insert also comprises a cell scaffold, a filter and a compression ring. The compression ring is sized and shaped to be received within the lower opening of the retaining ring. The cell scaffold and filter are received within the lower opening of the retaining ring and the cell scaffold is exposed to the upper opening of the retaining ring. The compression ring retains the scaffold and filter within the retaining ring against the narrower portion of the bore. A cell culture system comprising the one or more inserts, and a multiwell cell culture plate are also described herein.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The present application belongs to the technical field of biomaterial preparation, and particularly relates to a preparation method of a self-oxygen-supplying hydrogel cell scaffold, the obtained product and application. The present application realizes stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres. The method is realized through the following steps: compounds P-MA, P-Cys, ColMA and PEG-CaO2 are added to a PBS solution, a photoinitiator is added, and a'self-oxygen-supplying' composite hydrogel is obtained by irradiation with ultraviolet light of a wavelength of 365 nm. The present application provides a composite hydrogel loaded with oxygen-producing nanoparticles for 3D cell sphere culture and a new method for continuous release of O2 in a slightly acidic environment, aiming at the problems of intermediate cell death caused by hypoxia in the process of 3D culture of existing cell spheres, unclear matrigel composition, high price and the like. The present application solves the problem of internal hypoxia in the process of cell sphere culture, and clearly defines the gel composition for studying the interaction between other components and cells. In addition, the self-oxygen-supplying hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

Provided is a method for producing induced pluripotent stem cells with which it is possible to easily obtain induced pluripotent stem cells having good quality in a short time. A method for producing induced pluripotent stem cells according to the present invention comprises, in the following order: an inoculation step for inoculating somatic cells on a cell culture substrate provided with a cell scaffold material; a removal step for removing the suspended somatic cells that do not adhere to the cell scaffold material; and an introduction step for introducing a reprogramming factor into the somatic cells adhered to the cell scaffold material.

The invention belongs to the technical field of biology, and particularly relates to a cytoskeleton for promoting proliferation of corneal limbal stem cells. The raw materials of the cytoskeleton provided by the invention are chitosan and sodium hyaluronate, and the cytoskeleton has good biocompatibility and biological activity, can simulate the characteristics of an extracellular matrix, provides necessary physical and chemical support for stem cells, and does not cause immune response or cytotoxicity. The addition of the magnetic nanoparticles further enhances the performance of the cytoskeleton. Directional arrangement under the action of a magnetic field can provide structural support closer to an in-vivo microenvironment for cells, and adhesion and proliferation of the cells are promoted. The cell scaffold provided by the invention can provide a good growth microenvironment for the corneal limbus stem cells, and is beneficial to solving the problem that the corneal limbus stem cells are easy to lose activity and function in in-vitro culture.

The invention relates to the technical field of laser processing, in particular to a method and device for processing a high-longitudinal-width-ratio microstructure through axial shaping laser. According to the method, the complex coding hologram is loaded on the spatial light modulator, so that the axial intensity distribution of the Bessel beam can be accurately regulated and controlled, and the cylindrical voxel with the adjustable height can be prepared through single exposure. The voxel height can be continuously and gradually changed only by switching holograms generating Bessel beams with different focal lengths, and meanwhile, the maximum transverse size is kept unchanged. According to the method, frequent axial mechanical scanning or power adjustment is not needed in the machining process, and the machining efficiency and the operation stability can be improved; the method is suitable for rapid processing of microstructures with high longitudinal-width ratios, such as microcolumns, microtubes, cytoskeletons and binary diffraction devices.

The present invention relates to a cell culture insert 10 comprising the following features: a generally cylindrical retaining ring 12 for retaining the insert in a cell culture well (42 Fig. 3) and having upper 16 and lower 18 openings formed by a central bore 14 with a stepped profile (20, 22, 24 Fig. 2) providing a narrower portion of said bore from the lower opening; a cell scaffold 30; a filter 32; and a compression ring 34 sized and shaped to be received within the lower opening of the retaining ring; wherein the cell scaffold and filter are received within the lower opening of the retaining ring, the cell scaffold being exposed to the upper opening of the retaining ring, and wherein the compression ring retains the scaffold and filter within the retaining ring against said narrower portion (20 Fig. 2) of the bore. In preferred embodiments, a screw thread (Fig. 2) is provided on the exterior portion of the retaining ring.

The application discloses a three-dimensional cell scaffold based on a leaf vein of nandina domestica and a preparation method and application thereof, relates to the technical field of biomedical materials and tissue engineering, and discloses the following steps: taking the leaf vein of nandina domestica as a base material, and preparing the scaffold through alkali boiling, sodium periodate selective oxidation and amino-silica / chitosan composite modification. The scaffold retains the three-dimensional structure of the natural leaf vein completely, and significantly improves the cell compatibility of the material. Cell experiments prove that the scaffold has no cytotoxicity, can support the three-dimensional growth of mesenchymal stem cells and PC12 cells and the like with high survival rate, and effectively promotes cell proliferation. The method is simple, green, environmentally-friendly and low in cost, and the obtained scaffold has wide application prospects in the fields of tissue engineering, regenerative medicine and drug screening and the like.

A three-dimensional cell growth containment article is described, which includes a molded body channelized by removal of sacrificial channelizing element(s) therefrom, so that the molded body contains one or more channel(s) therein, with a matrix material in at least one of such channel(s) that is supportive of three-dimensional cell growth in the matrix material. A method for making such articles is also described, in which a molded body is formed with one or more sacrificial channelizing element(s) therein, following which the sacrificial channelizing element(s) are removed. The three-dimensional cell growth containment articles of the present disclosure may be utilized in any applications in which there exists a need to reproducibly generate three-dimensional cellular structures, e.g., islet transplantation for diabetes treatment, transplantation of hormone secreting cells, cellular scaffolds for wound healing, and generation of tissue engineering structures to regain structural usefulness for orthopedic applications.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

The application discloses a starch-based edible scaffold and a preparation method and application thereof, and belongs to the technical field of cell scaffold materials. The edible starch is used as a base material, and a semi-interpenetrating network (Semi-IPN) hydrogel scaffold is constructed through heat-induced self-assembly, and the scaffold has good biocompatibility and edible safety. The preparation method can accurately control the elastic modulus and mechanical properties of the scaffold, so that the scaffold is suitable for providing tensile stress stimulation for fish myoblasts. Thanks to the above-mentioned characteristics, the scaffold can not only effectively support the adhesion and proliferation of fish myoblasts, but also significantly promote the directional differentiation of the fish myoblasts, and form a biomimetic muscle tissue. The application provides an innovative and performance-controllable cell scaffold solution for producing fish culture meat products which do not need to be separated from the scaffold and can be directly eaten.

To provide a gelling agent capable of forming a gel composition having biocompatibility, biodegradability and cell adhesiveness.SOLUTION: The gelling agent includes a first polymer which is a derivative of a first biopolymer, a second polymer which is at least one selected from the group consisting of a second biopolymer and a derivative thereof, and a photopolymerization initiator. Of the first polymer and the second polymer, only the first polymer has a ureidopyrimidinone group, and only one of the first polymer and the second polymer has an ethylenically unsaturated group.SELECTED DRAWING: Figure 1

Provided is a cell scaffold material that can bring about good maintenance of memory T cells and good T cell proliferation.　A cell scaffold material according to the present invention is used for culturing a cell population containing T cells and contains a peptide-containing resin (A) having a synthetic resin part and a peptide part. The peptide part has a first peptide part and a second peptide part. The first peptide part and the second peptide part have different amino acid sequences. The first peptide part can bind to a first integrin, and the second peptide part can bind to a second integrin. The dispersive component γd of the surface free energy of the cell scaffold material is at least 24.5 mJ / m2 and not more than 45.0 mJ / m2, and the dipole component γp of the surface free energy of the cell scaffold material is at least 1.0 mJ / m2 and not more than 20.0 mJ / m2.

The invention relates to bioinformatics technology and specifically to an analysis method of a spatial transcriptome-based 4D-printed stem cell scaffold for enhancing diabetic skin injury healing. Spatial transcriptome sequencing is performed on tissues from both the PBS group and the combined treatment group. Cell clustering and annotation identify main cell types, followed by visualization of their spatial distributions in both groups. The proportions of various cell types are statistically quantified and plotted. Cell subsets exhibiting significant differential gene expression are enriched and verified. Using a 4D-printed stem cell scaffold combined with a piRNA inhibitor and stem cell therapy, the method reveals spatial distribution and dynamic cellular changes during wound healing. It identifies precise cellular locations and gene expression patterns within tissues, providing critical insights into the biological processes by which biomaterials promote diabetic injury repair. This approach offers new directions for future drug development.

Provided is a method for producing hematopoietic progenitor cells with which it is possible to stably obtain hematopoietic progenitor cells from pluripotent stem cells and with which it is possible to improve the efficiency of inducing differentiation from pluripotent stem cells into hematopoietic progenitor cells. This method for producing hematopoietic progenitor cells is provided with: a cell mass formation step in which pluripotent stem cells are cultured using a cell culture substrate provided with a substrate and a cell scaffold material arranged in a dot shape on a first surface of the substrate, and cell mass is formed on the surface of the cell scaffold material; and a differentiation induction step for inducing the differentiation of the obtained cell mass into a cell population containing hematopoietic progenitor cells.

The invention discloses a microsphere based on methacrylated gelatin, a preparation method of the microsphere and application of the microsphere in bio-ink, the microsphere is prepared by taking methacrylated gelatin as a core base material and selectively compounding glycosaminoglycan-methacrylated gelatin and / or lipidosome through a microfluidic technology, the size of the obtained microsphere is uniform, and the size of the microsphere is uniform. The composite components can endow the microspheres with excellent self-adhesion characteristics; a microfluidic technology is further adopted, a base material and a composite component are used as a microsphere shell phase, a cell dispersion liquid is used as a microsphere core phase, the cell-carrying microspheres with a core-shell structure are prepared, and the cell-carrying microspheres can well maintain normal survival and growth of embedded cells. The methylacryloylated gelatin-based microspheres or cell-loaded methylacryloylated gelatin-based microspheres can be used as 3D printing ink in the fields of preparation of scaffold materials, cell-loaded scaffold materials, adaptive cell three-dimensional culture, cell ball culture, high-density cell culture and tissue engineering implant materials.

Hydrogels that comprise one or more polymers and one or more milk proteins, wherein the polymer(s) and the milk protein(s) form an interconnected polymeric network via conjugation. In some embodiments, molecules of the polymer(s) are chemically modified to promote conjugation within the hydrogel. In some embodiments, molecules of the milk protein(s) are chemically modified to promote conjugation within the hydrogel. In some embodiments, molecules of both the polymer(s) and the milk protein(s) are chemically modified to promote conjugation(s) within the hydrogel to create the interconnected polymeric network. Examples of conjugation include, but are not limited to physical chain entanglement, ionic bonding, covalent bonding, secondary bonding, and hydrophobic-hydrophobic interactions, among others. The hydrogels can be enhanced with one or more cell-adhesion agents. The hydrogels can be used to form microcarriers and / or cell scaffolds for holding and proliferating living cells for creating comestible products, such as cultivated meat products, among others.

A method for preparing pet food by using animal-derived muscle stem cells, and a product obtained therefrom. The method comprises an integrated separation and culture technique for highly active muscle and adipose progenitor cells, and a large-scale three-dimensional culture and differentiation technique combined with a cell scaffold. The method can significantly improve the efficiency of separating myogenic and adipogenic progenitor cells and reduce costs.

The application belongs to the field of biomedical materials, and particularly relates to a recombinant humanized type I collagen, a DLP 3D printing biological ink, a preparation method and application, an amino acid sequence of the recombinant humanized type I collagen is shown as SEQ ID NO. 1; the biological ink comprises 50-150 mg / mL methacrylated recombinant humanized type I collagen, 2.0-5.0 mg / mL photoinitiator and 0.1-0.5 mg / mL light absorber; the biological ink has excellent printability and biological activity, a printed recombinant type I collagen scaffold has excellent biological activity, can significantly promote cell migration, proliferation and differentiation; a double-layer cell-loaded scaffold simulating a natural skin structure can be prepared, and the double-layer cell-loaded scaffold shows the effects of promoting epidermal regeneration and dermal remodeling, reducing a wound ROS level, improving a local immune microenvironment and enhancing angiogenesis, and has a good clinical application prospect.

The present invention relates to an inorganic / organic composite micro-patterned multicellular scaffold, its preparation method, and application. The inorganic / organic composite micro-patterned multicellular scaffold comprises a three-dimensional network-like scaffold framework formed by arranging inorganic / organic composite bio-ink loaded with cells A, and a scattered-point framework filled with hydrogel bio-ink loaded with cells B within the pore structure of the three-dimensional network-like scaffold framework.

The present invention is directed to a method for 3D printing of cells attached to a cell scaffold by simultaneous printing of a first composition comprising thermoresponsive microparticles and a second composition comprising cells. Further provided a printed 3D object such as an implant, comprising cells attached to the cell scaffold of the invention.

The invention relates to the technical field of preparation of medical stem cell preparations, and discloses a preparation method and a preparation system of a stem cell preparation for repairing joint injury, according to the method, autologous bone marrow MSCs are sorted through microfluidic, SOX9 genes are activated through CRISPR-Cas9 dual targeting, HLA-I / I is knocked out, then a bionic scaffold is subjected to 3D printing based on MRI data, and the stem cell preparation for repairing joint injury is obtained. The method comprises the following steps: fixing stem cells on a stent in a cross-linking manner, loading an exosome through CD63-amino covalent coupling, applying 0.5-1Hz periodic compression load culture according to a viscoelastic model, and performing gradient cryopreservation in a trehalose protective agent; the system comprises five modules: a stem cell pretreatment module, a bionic scaffold construction module, a cell-scaffold compounding module, a dynamic culture module and a cryopreservation quality inspection module. According to the method, the cartilage differentiation efficiency is improved by more than 3 times by utilizing gene editing, the exosome slow release period is prolonged to 14 days, meanwhile, the secretion amount of collagen I is improved by 89% through dynamic culture, and the freezing survival rate is greater than 96%; and the quality inspection qualification rate is 100%.