Kit for pancreas decellularized scaffold and preparation and reseeding methods of scaffold

An acellular scaffold and kit technology, applied in medical science, prosthesis and other directions, can solve the problems of inability to achieve replacement therapy, insufficient vascular network, simple structure of the pancreas, etc. Complete effect of cell depletion

Inactive Publication Date: 2015-02-18
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Pancreatic tissue engineering is an innovative way to construct a transplantable pancreas. However, due to the lack of suitable scaffold materials, the const

Method used

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  • Kit for pancreas decellularized scaffold and preparation and reseeding methods of scaffold
  • Kit for pancreas decellularized scaffold and preparation and reseeding methods of scaffold
  • Kit for pancreas decellularized scaffold and preparation and reseeding methods of scaffold

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Embodiment Construction

[0030] The kit for preparing the mouse whole pancreas bioscaffold in this example includes perfusate 1, perfusate 2 and perfusate 3.

[0031] Among them, the perfusion solution 1 is 0.25 g / L EDTA, 10000 U / L heparin sodium PBS buffer solution; the perfusion solution 2 is a mixture of 1% Triton-X 100 and 0.1% ammonia water, and the perfusion solution 3 is 0.01 g DNase Ⅰ.

[0032] Among them, the pH of the phosphate buffer solution in the perfusate 1 is 7.35; the pH of the solvent deionized water in the perfusate 2 and 3 is 7.35.

[0033] In this implementation, the preparation methods of perfusate 1, perfusate 2 and perfusate 3 are as follows:

[0034] The configuration of perfusate 1: Dissolve 125 mg EDTA and 0.4 ml heparin sodium in 1*PBS solution with a pH of 7.35, and dilute to 500 ml, of which 0.4 ml heparin sodium contains 10000 U heparin sodium.

[0035] The configuration of perfusate 2: Dissolve 10 ml Triton X-100 and 1 ml ammonia water in deionized water with pH=7.35, ...

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Abstract

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g/L of EDTA (ethylene diamine tetraacetic acid), 10,000 U/L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g/L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

Description

technical field [0001] The invention relates to a kit for pancreatic decellularized scaffolds, a preparation method of the scaffold and a replanting method. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia caused by defective insulin secretion or its biological action, or both. Diet adjustment, physical exercise, oral hypoglycemic drugs and exogenous insulin injection can reduce the incidence of diabetic microvascular and macrovascular lesions and related complications to a certain extent, but they cannot achieve a curative effect. Hyperglycemia-related organs Damage remains the leading cause of death in people with diabetes. [0003] β cells have the function of secreting insulin. Transplantation of pancreatic or islet cells is currently the only treatment that can achieve long-term stable control of blood sugar levels in patients with type 1 diabetes. At present, under the condition that the number of ideal pancreases ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38
Inventor 王志伟陆玉华郭益冰黄龑李晓红凌长春朱慧陆晶晶王雷邬迪范向军朱铭言
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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