13 results about "Decellularized scaffold" patented technology

The present invention provides A method of producing a decellularised extracellular matrix (ECM) scaffold of at least a portion of a lobular organ with no common artery, the method comprising: a) closing afferent blood vessels to substantially seal a target lobular organ or portion thereof with no common and / or major artery within a non-human donor or a dead / brain dead human donor; b) optionally: (i) cleaning coagulum and / or blood from at least a portion of the closed afferent blood vessels; and / or (ii) perfusing the organ or portion thereof to confirm closure of the afferent blood vessels; c) removing the sealed organ or portion thereof from the donor; and d) perfusing the sealed organ or portion thereof with detergent and enzymatic solutions to obtain the decellularised ECM scaffold. Methods for producing an artificial organ, and artificial organs produced by the methods are also provided.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

The present invention relates to decellularized plant tissue, methods for producing decellularized plant tissue, and the use of decellularized plant tissue in wound treatment. The method comprises the steps of: providing plant tissue, treating the plant tissue with an organic solvent, rinsing the plant tissue with a first wash buffer comprising an aqueous solution of a maximum of 3 wt. % of at least one surfactant, and treating the plant tissue with a second wash buffer comprising an aqueous solution of at least one bleaching agent, characterized in that the rinsing takes place in an adaxial-abaxial or abaxial-adaxial direction.

This application relates to a decellularized scaffold for cartilage repair in the biomedical field, its preparation method, and application. The preparation method comprises: preparing a decellularized matrix from animal cartilage; chemically bonding the decellularized matrix with a lipid compound to obtain a double-bond-modified decellularized matrix; copolymerizing and cross-linking the double-bond-modified decellularized matrix to obtain a decellularized matrix hydrogel; and immersing the decellularized matrix hydrogel in a dipeptide solution for modification to obtain the decellularized scaffold. The decellularized scaffold of this application introduces both mechanical and electrical stimulation effects into the scaffold, addressing deficiencies in previous research and demonstrating good biocompatibility and repair effectiveness in both in vivo and in vitro experiments.

This invention relates to the field of biomedical materials technology, providing a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold material, its preparation method, and its applications. The preparation method includes: decellularizing adipose tissue to obtain adipose tissue decellularized scaffold; heparinizing the scaffold to obtain a heparinized adipose tissue decellularized scaffold; loading hepatocyte growth factor and vascular endothelial cell growth factor onto the scaffold to obtain a heparinized dual-growth factor adipose tissue decellularized scaffold; seeding induced pluripotent stem cell-derived hepatocytes onto the scaffold, and culturing to obtain a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold (RAT). This invention uses rat inguinal adipose tissue to prepare RAT material, which is low-cost, readily available, has a simple preparation process, and is easy to scale up. The RAT material of this invention can achieve liver tissue damage repair and hepatocyte regeneration, providing a new treatment strategy for acute liver failure.

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

The invention discloses a preparation method of fat-derived decellularized scaffold hydrogel. The method comprises the following steps: 1, shearing a DAT block into chippings, and carrying out ball milling to obtain DAT powder; 2, adding DAT powder into an HCl solution, and then adding pig gastric mucosa pepsin for enzymolysis to obtain an enzymatic hydrolysate system; 3, adjusting the pH and temperature of the enzymatic hydrolysate system, and then adding methacrylic anhydride Ma for grafting to obtain a grafting liquid system; 4, performing centrifugal dialysis on the grafting liquid system, and performing freeze drying to obtain a DAT hydrogel precursor DM; and 5, dissolving the DAT hydrogel precursor DM, adding an initiator, and irradiating under ultraviolet light to obtain the DM hydrogel. On the basis of physical, chemical and enzymolysis methods, the water solubility of the fat-derived decellularized scaffold DAT is improved, then methacrylic anhydride is grafted, the photo-crosslinking reaction is performed, the preparation of the hydrogel based on the fat-derived decellularized scaffold is realized, and the hydrogel has a three-dimensional porous structure and is suitable for the field of tissue repair.

The invention provides a bioengineering kidney construction method based on an organoid and a decellularized scaffold, and the method comprises the following steps: injecting blood vessel promoting stem cells into a kidney decellularized scaffold material, carrying out fluid circulation perfusion culture for several days, and after the kidney decellularized scaffold material is re-cellularized, injecting a kidney organoid (KIO) into the scaffold material for perfusion culture. The bioengineering kidney obtained by the method disclosed by the invention can be used as a graft for replacing renal functions. The invention also provides a kidney organoid culture amplification method, the organoid obtained by the method not only contains kidney parenchymal cells such as glomerular cells and renal tubule cells, but also contains blood vessels and immune cells, and a good kidney parenchymal cell source can be provided for tissue engineering kidney preparation.

The present invention relates to a method for producing a highly functional artificial organ using aptamers, comprising blood vessels capable of transporting perfusate. When used as a coating agent for a decellularized scaffold, the aptamer according to the present invention enhances vascular adhesion, activity, and angiogenic potential, thereby enabling more effective vascular reconstruction than existing antibodies. Consequently, a vascularized artificial liver prepared using this aptamer reduces thrombosis in vivo and enhances liver function, and is therefore expected to be applicable to methods for treating diseases using artificial organs prepared using the aptamers according to the present invention.

To provide a composition for producing artificial organs comprising aptamers, to provide a composition for blood vessel coating comprising aptamers, to provide a method for producing artificial organs using aptamers, and to provide the produced artificial organs.SOLUTION: The present invention provides a composition for producing artificial organs comprising aptamers. When the aptamers are used as a coating agent of a decellularized scaffold, the composition enhances blood vessel adhesion ability, viability, and angiogenesis potential, thereby enabling more efficient reconstruction of vasculature than existing antibodies.SELECTED DRAWING: Figure 5a

The invention discloses a thymus decellularized scaffold as well as a preparation method, a kit and application thereof. The method comprises the following steps: 1) flushing thymus with a cleaning agent; 2) washing the cleaned thymus with a detergent A; 3) washing the washed thymus with a detergent B; 4) washing the washed thymus with a preservative; 5) cleaning the flushed thymus by using a cleaning agent; and 6) adding a preservative to prepare the thymus decellularized scaffold. The cleaning agent contains EDTA, antibiotics and PBS; the detergent A contains lauryl sodium sulfate and antibiotics; the detergent B contains anhydrous magnesium sulfate, anhydrous calcium chloride, Triton X-100 and antibiotics; and the preservative contains antibiotics and PBS. The method for amplifying thymus stromal cells in vitro based on the thymus decellularized scaffold is beneficial to directional induction of development and differentiation of hematopoietic stem progenitor cells into T cells, and has wide application potentials in large-scale culture of T cells in the future and in the field of T cell immunotherapy.

The present invention provides a method of producing a decellularised extracellular matrix (ECM) scaffold of at least a portion of a lobular organ with no common artery, the method comprising: a) closing afferent blood vessels to substantially seal a target lobular organ or portion thereof with no common and / or major artery within a non-human donor or a dead / brain dead human donor; b) optionally: (i) cleaning coagulum and / or blood from at least a portion of the closed afferent blood vessels; and / or (ii) perfusing the organ or portion thereof to confirm closure of the afferent blood vessels; c) removing the sealed organ or portion thereof from the donor; and d) perfusing the sealed organ or portion thereof with detergent and enzymatic solutions to obtain the decellularised ECM scaffold. Methods for producing an artificial organ, and artificial organs produced by the methods are also provided.

The present invention provides compositions and methods for producing insulin beta cells from pluripotent stem cells. The compositions and methods of the invention relate to stepwise differentiation when differentiated cells are cultured on lung tissue-derived decellularized scaffolds.