45 results about "Decellularized scaffold" patented technology

The invention discloses a method for linking a RGD improved acellular tissue engineering valve / intravascular stent with epoxy chloropropane. The method, by using epoxy groups of the epoxy chloropropane, cross links and fixes the functional domain RGD (YGRGDSP) polypeptide of cell adhesion and recognition receptors on an acellular tissue engineering valve / intravascular stent material; meanwhile, the chemical crosslinking function of the epoxy chloropropane can fully cross link the collagen protein which is the main component of the acellular stent material so as to achieve the purposes of anti-calcification and reducing the zymolysis speed inside the stent material, and finally proving the improved acellular tissue engineering valve / intravascular stent with better biological and mechanical properties, higher adhesion rate toward seed cell, and better property of delaying enzyme digestion and anti-calcification, therefore, the acellular tissue engineering valve / intravascular stent can actually meet the requirements of tissue engineering valves.

The invention discloses a kit for obtaining a multiple-organ and tissue extracellular matrix and a using method of the kit. The kit for obtaining the multiple-organ and tissue extracellular matrix comprises a nonionic detergent I, an ionic detergent I, an ionic detergent II, a hypertonic solution, a hypotonic solution, an isotonic solution, a nucleic acid removal agent and a tissue bulking agent. According to the kit and a method for using the kit provided by the invention, acellularized scaffolds of multiple organs and tissues can be obtained in a short time, and the structure and most of structure of the extracellular matrix are maintained, so that product materials are provided for research and application of regenerative medicines and tissue engineering.

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g / L of EDTA (ethylene diamine tetraacetic acid), 10,000 U / L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g / L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

The invention discloses a construction method of a three-dimensional liver microtissue unit for a bio-artificial liver support system. The construction method is characterized by comprising the steps that decellularized scaffold components of a pig liver are encapsulated by microcapsules prepared from sodium alginate-polycations, liver cells, vascular endothelial cells and fibroblasts are composited, and the functional three-dimensional liver microtissue unit is constructed through cell self-assembly and applied to the bio-artificial liver support system. According to the method, operation is easy, cost is low, the liver cells, the vascular endothelial cells and the fibroblasts are self-assembled into three-dimensional liver microtissue by simulating interaction between the cells and an extracellular matrix, interaction between the cells and soluble factors and interaction between the cells and the cells in a liver tissue microenvironment, the three-dimensional liver microtissue has good synthesis, secretion and detoxification functions, and therefore the three-dimensional liver microtissue can serve as the microunit to be applied to the bio-artificial liver support system.

The invention relates to an artificial nerve graft constructed based on a chip-type acellularized scaffold and a preparation method of the artificial nerve graft, belonging to the field of tissue engineering. According to the artificial nerve graft and the preparation method, heterogeneous animal medulla spinalis, skeletal muscle or tendon acellularized tissue slices are adopted as a bracket, genipin is adopted for crosslinking cytokines, autologous ecto mesenchymal stem cells are planted on the bracket, and fibrous protein is taken as an adhesive for constructing the artificial nerve graft, so that the repairing of the nervous tissue is promoted. The artificial nerve graft and the preparation method have the advantages that the preparation cost is low, the period is short, the preparation raw materials are easily available, and the preparation is simple and is easy to operate. The constructed artificial nerve graft has low immunogenicity, contains a large number of autologous stem cells and cell factors, and is beneficial for adjusting the nerve injury microenvironment, and promoting the nerve regeneration and myelinogenesis. The artificial nerve graft can be used for preparing peripheral nerve and medulla spinalis defect, and has the broad application prospect.

The invention discloses a preparation method of acellular matrix hydrogel. The preparation method comprises the following steps: obtaining a tissue block; transferring the tissue block into a solutioncontaining 0.02% of pancreatin and 0.05% of ethylenediamine tetraacetic acid, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 3% of Triton X, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 4% of sodium deoxycholate, and performing stirring for 2-3 h; moving the tissue block into deionized water to be soaked for 12-18 h to obtain a decellularized scaffold; transferring the decellularized scaffold into 75% alcohol to be soaked for 30 min, transferring the decellularized scaffold into a solution containing 0.1% peracetic acid, and carrying out stirring for 2-3 h; freeze-drying the decellularized scaffold, grinding the decellularized scaffold into matrix powder, transferring the matrix powder into a solution containing pepsase hydrochloride, and performing stirring for digestion for 2-3 d to obtain pre-gel; and diluting the pre-gel to a preset concentration, and performing standing to obtain the acellular matrix hydrogel. The acellular matrix hydrogel is efficiently extracted from umbilical cord tissue, and the integrity of the internal structure of the acellular matrix is reserved to the maximum extent.

The invention provides a decellularized collagen gel microsphere scaffold preparation method, and belongs to the technical field of decellularized scaffold tissue engineering. The decellularized collagen gel microsphere scaffold preparation method comprises preparation of a cell-collagen mixing liquid, preparation of cell-collagen gel microspheres, and preparation of a decellularized collagen gel microsphere scaffold. According to the present invention, by directly preparing the cell coating collagen gel microspheres, the micrometer-scale tissue block is obtained through in vitro culture, such that the decellularization operation can be directly performed while a large amount of the micrometer-scale tissue can be obtained, the subsequent breaking process is avoided, and the matrix and the micro-structure in the tissue are well retained; the decellularized collagen gel microsphere scaffold does not have DNA residue so as to minimize the rejection reaction; and the size of the decellularized collagen gel microsphere scaffold is micrometer-scale, and after the decellularized collagen gel microsphere scaffold is used in cartilage and subchondral bone defect repair, the cell adhesion and the tissue healing are easily achieved, the uniformly distributed tissue is formed at the late stage, the hollowing can be avoided, and the probability of surgical trauma and late stage infections can be reduced.

The invention discloses a preparation method of novel tissue engineering skin. The method includes steps of jointly culturing an amniotic membrane decellularized scaffold and a mesenchymal stem cell to be a carrier, inoculating horn cell; culturing by a serum-free culturing system, and acquiring a complete tissue engineering skin membrane. Through the method, the method can culture a complete skin membrane and the skin membrane can be applied to the skin scar repair and leucoderma treatment. The horn cell is originated from body skin or prepuce of the newborn. The carrier is low in immunogenicity while also has biological activity; the serum-free culturing system can realize the in-vitro serum-free cultivation and amplification of the horn cell, and improve cell growth, multiplication, cell form, activity and function instead of bad influence; the skin membrane cultivated by the method is approached to natural skin; after the skin-grafting culturing, it can be applied to the scar repair of scaled and other injured skins as well as treatment of skin disease such as leucoderma.

The invention discloses an injectable decellularized scaffold for cartilage repair as well as a preparation method and application of the injectable decellularized scaffold, and relates to the technical field of medical materials. According to the method, a double-injection mode is adopted, and the gelated gradient injectable decellularized scaffold is obtained by injecting a pre-gel solution and a viscosity modifier simultaneously. The decellularized scaffold can load MSCs to be implanted into a body, is hydrolyzed under a physiological pH value, and slowly releases a medicine at an injured part, so that the differentiation of the loaded MSCs to cartilage cells is promoted, and the injured cartilage tissue is repaired; Due to the gradient property, original biological characteristics of the cartilage tissue can be better simulated, and a better repairing effect is achieved. According to the decellularized scaffold and the technology, the raw materials are cheap and wide in source, after clinical popularization, the pain of a patient can be remarkably relieved, the operation frequency is reduced, the treatment effect is improved, and remarkable social and economic benefits are achieved.

Belonging to the field of biological tissue engineering and medical materials, the invention relates to a decellularized cerebellar extracellular matrix regeneration biological scaffold containing multiple neurotrophic factors and a preparation method thereof. As a cerebellum regeneration scaffold material, the natural cerebellar decellularized scaffold adopted by the invention is a three-dimensional network structure, and the spatial configuration conforms to the cerebellar physiological structure spatial configuration. The regeneration biological scaffold and its biomechanical properties are similar to normal cerebellus, and can provide physiologically similar channels for reconstruction of nerve pathways so as to provide correct guidance for growth. The scaffold surface has cell-membrane receptor recognition sites and partially active factors, thus being conducive to adhesion growth of cells and functioning of physiological functions. Engineered seed cells can secrete a variety of neurotrophic / growth factors, also can promote the formation of new extracellular matrixes, and provides regeneration guidance signals. With good biocompatibility, the regeneration biological scaffold can be used for preparation of neural repair preparations for treatment of nerve injuries.

The invention provides a bacteriostatic hydrogel dressing material for wound repair and a preparation method thereof. The bacteriostatic hydrogel dressing material provided by the invention is solid-state fine powder consisting of epsilon-polylysine, gamma-polyglutamic acid, acellularized scaffold powder and cross-linking agents; and the bacteriostatic hydrogel dressing can be directly spread on the wound surface or can be pasted onto the wound surface after absorbing certain water to become gel. According to composition ingredients, the mass ratio of the epsilon-polylysine to the gamma-polyglutamic acid is (100:1) to (1:10); the acellularized scaffold powder is 0.01 to 20 percent of the total mass of the epsilon-polylysine and the gamma-polyglutamic acid; and the cross-linking agents account for 0.01 percent to 40 percent of the weight of the hydrogel dry powder dressing. The wound repair material has good hygroscopicity; the water absorption capability reaches 200 times of the self weight; the wound repair material also has good bacteriostatic capability, so that the additional addition of antibiotics or silver-containing bacteriostatic ingredients is not needed; and meanwhile, good mechanical performance is also realized. In addition, the bacteriostatic hydrogel dressing material has the advantages that the preparation method is simple; the repeatability is good; and the large-scale production can be easily realized.

A preparing method of a small-intestine decellularized scaffold with a complete three-dimensional structure and a vascular network is disclosed. The method is characterized by comprising following steps: a first step of selecting a healthy adult mammal, anesthetizing, injecting with an anticoagulant, opening the abdominal cavity, subjecting the small intestine section to cannula fixation for artery and vein vessels, and ligating at the proximal end to break the vessels; a second step of clipping two ends of the small intestine section, breaking the two ends of the small intestine section, respectively performing cannula fixation, separating a mesentery, taking down a complete intestinal tube and a complete mesenteric vessel which are needed, putting into a low-temperature environment, and repeatedly washing with normal saline; a third step of perfusing the artery, the vein and the intestinal tube with a phosphate buffer, a trypsin solution containing ethylenediaminetetraacetic acid, the phosphate buffer, a sodium dodecylsulfate solution, the phosphate buffer, a polyethylene glycol octylphenol ether solution and the phosphate buffer in order; and a fourth step of disinfecting after perfusion. The small-intestine decellularized scaffold prepared by the method can maintain a complete scaffold structure.

The invention provides a biological culture device for culturing a tissue engineered lung. The biological culture device comprises a main vessel, an auxiliary vessel and a breathing simulation device, wherein the main vessel is used for storing a tissue culture solution and a biological material, and the biological material can be subjected to cell regenerative culture in the tissue culture solution; the auxiliary vessel is used for storing a small airway culture solution and is connected with the main vessel through a first guide pipe to form a closed airway breathing loop; the breathing simulation device is connected to the main vessel through a second guide pipe and is used for simulating the negative-pressure ventilation for the biological material. The biological culture device provided by the invention is simple in operation and can be used for simulating the characteristics (blood perfusion and liquid ventilation) of fetal lung development, creating culture and development environments and a physical stimulation environment for culturing the tissue engineered lung and ensuring that an acellularized scaffold is cultured in a simulated physiological environment with similar mechanical tension and necessary components for similar normal fetal blood circulation, breathing movement and fetal lung growth and development.

The invention discloses a tissue accellular solution. The tissue accellular solution contains lauryl sodium sulfate and ascorbic acid which are soluble in a buffer solution. The invention further discloses omentum majus tissue processed through the tissue accellular solution. Through antioxidation of the ascorbic acid (vitamin C), oxidative damage to acellular matrix in the accellular process is reduced, extracellular metrix protein in the acellular matrix is protected, and the biocompatibility of a decellularized scaffold is improved. The method has the advantages that operation is easy, cost is low, and the feasibility is high.

The invention discloses use of salidroside and analogue thereof in dynamically filling regenerated pancreas islet in a pancreatic decellularized scaffold, specifically application of salidroside and analogue thereof in preparing reagents for resisting pancreas islet mechanical damages and center anoxia, and promoting a pancreas islet function in dynamically filling regenerated pancreas islet in athree-dimensional mode in the pancreatic decellularized scaffold. The effect is exact.

The invention relates to an artificial nerve graft constructed based on a chip-type acellularized scaffold and a preparation method of the artificial nerve graft, belonging to the field of tissue engineering. According to the artificial nerve graft and the preparation method, heterogeneous animal medulla spinalis, skeletal muscle or tendon acellularized tissue slices are adopted as a bracket, genipin is adopted for crosslinking cytokines, autologous ecto mesenchymal stem cells are planted on the bracket, and fibrous protein is taken as an adhesive for constructing the artificial nerve graft, so that the repairing of the nervous tissue is promoted. The artificial nerve graft and the preparation method have the advantages that the preparation cost is low, the period is short, the preparation raw materials are easily available, and the preparation is simple and is easy to operate. The constructed artificial nerve graft has low immunogenicity, contains a large number of autologous stem cells and cell factors, and is beneficial for adjusting the nerve injury microenvironment, and promoting the nerve regeneration and myelinogenesis. The artificial nerve graft can be used for preparing peripheral nerve and medulla spinalis defect, and has the broad application prospect.

The invention belongs to the technical field of repairing of injury of bone tendon interfaces, in particular to a double-bionic page-shaped scaffold for a bone tendon interface. A preparation method of the double-bionic page-shaped scaffold for the bone tendon interface comprises the following steps: step 1, acquiring normal bone tendon interface tissue samples; and step 2, preparing the double-bionic page-shaped scaffold for the bone tendon interface, exactly, (1), decalcifying the bone tendon interface tissues, (2) preparing the scaffold by adopting page-shaped multilayered tissue slices, and (3), performing decellularization treatment on the bone tendon interface scaffold. By adopting the double-bionic scaffold, structures and matrix components of the tissue can be maintained, and lossof extracellular matrix components also can be avoided to a certain extent, particularly, the loss of biological active ingredients; and moreover, the phenomenon that a decellularization scaffold hasthe excellent traction mechanical performance also can be ensured, and quick and firm anchoring of bones and tendons can be realized. In addition, a decellularization process of the tissues can be effectively quickened, the decellularization time is reduced, and morphological structures and the matrix components of the tissues are maintained preferably.

The invention discloses a platelet-rich plasma(PRP)-loaded pancreatic decellularized scaffold and a preparation method thereof and belongs to the technical field of pancreatic tissue engineering. PRPrich in various vascularization promoting growth factors is assembled in vessels of the pancreatic decellularized scaffold while human umbilical vein endothelial cell is planted in the vessels of thepancreatic decellularized scaffold, the loaded PRP is activated slowly by collagenous fiber of the pancreatic decellularized scaffold to releases a great quantity of growth factors slowly continuouslylocally, thereby providing an ideal environment for adhesion and proliferation of the human umbilical vein endothelial cell surrounding the inherent vascular networks in the scaffold; the PRP loadedpancreatic decellularized scaffold keeps the natural structure, biological characteristics and low immunogenicity of the pancreatic tissue; the growth factors which are slowly released continuously locally after the scaffold is transplanted in vivo have a remarkable vascularization promoting function; the scaffold is convenient to operate and has good controllability; generation of new vessels inthe scaffold is facilitated.

The invention discloses a reendothelialization pancreas decellularized scaffold, and a preparation method thereof. According to the preparation method of the reendothelialization pancreas decellularized scaffold, assembling of a sulfuric acid polysaccharide with anticoagulation effect in vessels of pancreas decellularized scaffold is carried out so as to obtain the reendothelialization pancreas decellularized scaffold. The anticoagulation effect of the reendothelialization pancreas decellularized scaffold is achieved at local parts, and is controllable, so that it is beneficial for capturing of endothelial progenitor cells and promoting of scaffold reendothelialization.

The invention discloses a decellularized scaffold and a preparation method thereof.The preparation method comprises the steps that decellularized bone powder is prepared, the decellularized bone powder, hydroxyl calcium polyphosphate and PLGA are mixed and then subjected to electrostatic spinning, and a carrier scaffold is obtained; soaking the carrier scaffold in a basic fibroblast growth factor solution to obtain a basic fibroblast growth factor-loaded carrier scaffold; and inoculating osteoblasts on the carrier scaffold loaded with the basic fibroblast growth factors to obtain the decellularized scaffold. According to the preparation method, cells and fat can be efficiently removed through a specific decellularization method, then decellularized bone particle powder, hydroxyl calcium polyphosphate and PLGA are subjected to electrostatic spinning to prepare the biological scaffold, the scaffold is made to have good biocompatibility, pore diameter structure and mechanical strength, and then the biological scaffold can be used for preparing the biological scaffold. And basic fibroblast growth factors and osteoblasts are loaded, so that proliferation and differentiation of the osteoblasts can be promoted, and an osteogenesis promoting effect is achieved.

The invention discloses a tissue accellular solution. The tissue accellular solution contains lauryl sodium sulfate and ascorbic acid which are soluble in a buffer solution. The invention further discloses omentum majus tissue processed through the tissue accellular solution. Through antioxidation of the ascorbic acid (vitamin C), oxidative damage to acellular matrix in the accellular process is reduced, extracellular metrix protein in the acellular matrix is protected, and the biocompatibility of a decellularized scaffold is improved. The method has the advantages that operation is easy, cost is low, and the feasibility is high.

The invention provides a cellularization biological liver stent with an anticoagulation property and a preparation method of the cellularization biological liver stent. The preparation method comprises the following steps: (a) pouring a solution containing heparin and / or heparinate into the cellularization biological liver stent, wherein the pouring speed is 80mL / min-150mL / min, and the pouring time is 5-12 hours; and (b) carrying out sterilization. The prepared cellularization biological liver stent is strong in anticoagulation property and good in cytocompatibility and plays a very important support role in the survival, differentiation and proliferation of cells planted on the cellularization biological liver stent, and a convenient and cheap source and the preparation method are provided for the construction of a heterologous tissue-engineered biological anti-coagulation stent with a clinical application value.

The invention provides a decellularized cartilage support. The cartilage support is prepared from the following main components: silk fibroin, snail mucin, sterol and decellularized scaffold component of cartilage of toads or frogs; the mechanical strength and degradation speed of the cartilage support are controlled by changing the component proportion; the decellularized cartilage support is in a three-dimensional solid shape and has good mechanical property; tiny and dense holes are distributed in the surface of the decellularized cartilage support, so that the decellularized cartilage support is beneficial to adhesion and regeneration of cartilage cells. In a preparation process of the decellularized cartilage support, heating is not carried out, and a chemical or enzyme crosslinking agent is not used, so that the activities of all the components are maintained. The decellularized cartilage support gives play to the collaborative advantage of all the components, and is applied to substitute transplants of cartilage tissues.

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction,such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

The invention provides a method for preparing a porous extracellular matrix scaffold for tissue engineering. In the method, a nanometer or micrometer polymer scaffold is implanted into the subcutaneous or peritoneal cavity of a host, and the polymer scaffold is used as a template to prepare a tissue-engineered porous extracellular matrix scaffold using the immune protection mechanism of the host. Controlled pore structure, and non-immunogenic tissue engineered extracellular matrix scaffold implanted in vivo. The invention solves the problem of pore formation of extracellular matrix scaffold materials; the porous scaffold is derived from the animal itself and is used for repairing autologous damaged tissues or organs without immune rejection; at the same time, since there is no complicated decellularization step for autologous repair, the scaffold The mechanical strength of the cell matrix is ​​obviously better than that of the decellularized scaffold material; the cell matrix porous scaffold can be further decellularized for allogeneic tissue or organ repair, and has a good application prospect in the field of organ repair.

The invention discloses a preparation method of novel tissue engineering skin. The method includes steps of jointly culturing an amniotic membrane decellularized scaffold and a mesenchymal stem cell to be a carrier, inoculating horn cell; culturing by a serum-free culturing system, and acquiring a complete tissue engineering skin membrane. Through the method, the method can culture a complete skin membrane and the skin membrane can be applied to the skin scar repair and leucoderma treatment. The horn cell is originated from body skin or prepuce of the newborn. The carrier is low in immunogenicity while also has biological activity; the serum-free culturing system can realize the in-vitro serum-free cultivation and amplification of the horn cell, and improve cell growth, multiplication, cell form, activity and function instead of bad influence; the skin membrane cultivated by the method is approached to natural skin; after the skin-grafting culturing, it can be applied to the scar repair of scaled and other injured skins as well as treatment of skin disease such as leucoderma.

The invention provides a preparation method of a tissue engineering liver based on a plant decellularized scaffold. The preparation method comprises the following steps: S1, cutting out a stem part of hollow oenanthe javanica; s2, immersing the stem part of the celery into a lauryl sodium sulfate solution, and then rinsing the stem part of the celery by utilizing trition x-100 containing hypochlorous acid, so as to obtain a decellularized scaffold; and S3, planting the hepatocytes on the acellular scaffold and culturing the hepatocytes.

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g / L of EDTA (ethylene diamine tetraacetic acid), 10,000 U / L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g / L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

The invention discloses a method for constructing a three-dimensional micro-liver tissue unit used in a bioartificial liver support system. It is characterized by adopting microcapsules prepared by sodium alginate-polycation to encapsulate porcine liver decellularized scaffold components, compounding hepatocytes, vascular endothelial cells and fibroblasts, and constructing functional three-dimensional micro-liver tissue units through cell self-assembly. And applied to the bioartificial liver support system. The invention is simple in operation and low in cost, and by simulating the interaction between cells and extracellular matrix, cells and soluble factors, and cells in the microenvironment of liver tissue in vivo, the self-assembly of liver cells, vascular endothelial cells and fibroblasts into The three-dimensional micro-liver tissue has good synthesis, secretion and detoxification functions, so it can be used as a micro-unit in a bioartificial liver support system.