14 results about "Acellular scaffold" patented technology

The invention relates to a preparation method and application of acellular extracellular matrix hydrogel for organoid culture, and the method comprises the following steps: step (1): preparing a small intestine acellular scaffold: sequentially putting pig small intestines without adipose tissues into a first solution, a second solution, a third solution and a fourth solution, stirring, and freezing and freeze-drying to obtain the small intestine acellular scaffold; step (2): preparing small intestine acellular matrix hydrogel, cutting a small intestine acellular scaffold into small pieces or grinding the small intestine acellular scaffold into powder, preparing a small intestine acellular scaffold solution with a hydrochloric acid solution and a pepsase solution, centrifuging, adding a PBS solution to terminate digestion, and adjusting the pH value to 6.0-8.0 to obtain solution state small intestine acellular matrix hydrogel; and gelling the solution-state small intestine extracellular matrix hydrogel at the temperature of 37 DEG C for 20-60 minutes to obtain the gel-state small intestine extracellular matrix hydrogel which is used for culturing alimentary canal organs.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

The present invention relates to decellularized plant tissue, methods for producing decellularized plant tissue, and the use of decellularized plant tissue in wound treatment. The method comprises the steps of: providing plant tissue, treating the plant tissue with an organic solvent, rinsing the plant tissue with a first wash buffer comprising an aqueous solution of a maximum of 3 wt. % of at least one surfactant, and treating the plant tissue with a second wash buffer comprising an aqueous solution of at least one bleaching agent, characterized in that the rinsing takes place in an adaxial-abaxial or abaxial-adaxial direction.

The invention provides a functionalized cornea stroma lens acellular scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. A functional cornea-derived natural extracellular matrix (ECM) scaffold is used as a core, a decellularized freeze-drying technology is combined to prepare a decellularized freeze-dried lens (LDL) convenient to store, and an oxidized heparin interface modification technology is used to enrich active factors through an electron capture principle so as to construct a functional ECM platform. And the safety and effectiveness are evaluated in vivo and in vitro. Research results show that the system has long-term storage capacity and low immunogenicity, can prolong the action duration of a nerve growth factor (NGF), effectively inhibits postoperative inflammation and fibrosis in a rabbit cornea stroma lens transplantation model, supports stroma cell colonization and nerve reinnervation, and remarkably promotes cornea tissue repair and regeneration. The novel functional ECM platform developed by the invention provides a new thought and reference for the ECM stent in the field of cornea regeneration treatment.

The present disclosure provides a bioengineered kidney construction method based on organoids and acellular scaffolds, which comprises first injecting pro-vascular stem cells into a kidney acellular scaffold material, and then culturing for several days through fluid circulation perfusion, and then injecting kidney organoids (KIO) into the scaffold material for perfusion culture after the kidney acellular scaffold material is recellularized. The bioengineered kidney obtained by the method of the present disclosure can be used as a graft for replacing kidney function. The present disclosure also provides a kidney organoid culture and expansion method, and the organoids obtained by the method contain not only kidney parenchymal cells such as glomerular cells and renal tubular cells, but also blood vessels and immune cells, which provides a good source of kidney parenchymal cells for the preparation of tissue-engineered kidneys.

This application relates to a decellularized scaffold for cartilage repair in the biomedical field, its preparation method, and application. The preparation method comprises: preparing a decellularized matrix from animal cartilage; chemically bonding the decellularized matrix with a lipid compound to obtain a double-bond-modified decellularized matrix; copolymerizing and cross-linking the double-bond-modified decellularized matrix to obtain a decellularized matrix hydrogel; and immersing the decellularized matrix hydrogel in a dipeptide solution for modification to obtain the decellularized scaffold. The decellularized scaffold of this application introduces both mechanical and electrical stimulation effects into the scaffold, addressing deficiencies in previous research and demonstrating good biocompatibility and repair effectiveness in both in vivo and in vitro experiments.

This invention relates to the field of biomedical materials technology, providing a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold material, its preparation method, and its applications. The preparation method includes: decellularizing adipose tissue to obtain adipose tissue decellularized scaffold; heparinizing the scaffold to obtain a heparinized adipose tissue decellularized scaffold; loading hepatocyte growth factor and vascular endothelial cell growth factor onto the scaffold to obtain a heparinized dual-growth factor adipose tissue decellularized scaffold; seeding induced pluripotent stem cell-derived hepatocytes onto the scaffold, and culturing to obtain a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold (RAT). This invention uses rat inguinal adipose tissue to prepare RAT material, which is low-cost, readily available, has a simple preparation process, and is easy to scale up. The RAT material of this invention can achieve liver tissue damage repair and hepatocyte regeneration, providing a new treatment strategy for acute liver failure.

The application relates to the field of tissue engineering and regenerative medicine, and particularly discloses a cell-extracellular matrix complex for hair follicle regeneration and a preparation method thereof. The complex comprises autologous hair follicle stem cells, a human scalp-derived acellular ECM scaffold, time-sequentially added growth factors and an anti-scarring component. In preparation, the autologous hair follicle stem cells are first induced by BMP4 and TGF-beta 1 in cooperation, then inoculated into the human scalp-derived acellular ECM scaffold, and the complex is constructed under dynamic culture conditions, and VEGF is added to promote vascularization. The complex can efficiently form a structure-intact hair papilla-like tissue, has good biocompatibility, low immunogenicity and anti-scarring characteristics, and is significantly superior to a traditional collagen scaffold system. The application provides a new regeneration treatment strategy with clear clinical transformation prospects for scaleable preparation for androgenetic alopecia and other hair follicle deficiency diseases.

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

The present invention provides a method of producing a thymic construct suitable for implantation into a subject, the method comprising the steps of: (i) providing a decellularised scaffold; (ii) seeding the decellularised scaffold with thymic epithelial cells having mesenchymal properties; and (iii) culturing the seeded scaffold to produce the construct. The thymic epithelial cells are preferably CD49f + , also VIM + , TE-7 + , and / or CD90 + . The present invention further provides pharmaceutical compositions, uses and therapies using thymic epithelial cells having mesenchymal properties.

The invention provides a bioengineering kidney construction method based on an organoid and a decellularized scaffold, and the method comprises the following steps: injecting blood vessel promoting stem cells into a kidney decellularized scaffold material, carrying out fluid circulation perfusion culture for several days, and after the kidney decellularized scaffold material is re-cellularized, injecting a kidney organoid (KIO) into the scaffold material for perfusion culture. The bioengineering kidney obtained by the method disclosed by the invention can be used as a graft for replacing renal functions. The invention also provides a kidney organoid culture amplification method, the organoid obtained by the method not only contains kidney parenchymal cells such as glomerular cells and renal tubule cells, but also contains blood vessels and immune cells, and a good kidney parenchymal cell source can be provided for tissue engineering kidney preparation.

The present invention relates to a method for producing a highly functional artificial organ using aptamers, comprising blood vessels capable of transporting perfusate. When used as a coating agent for a decellularized scaffold, the aptamer according to the present invention enhances vascular adhesion, activity, and angiogenic potential, thereby enabling more effective vascular reconstruction than existing antibodies. Consequently, a vascularized artificial liver prepared using this aptamer reduces thrombosis in vivo and enhances liver function, and is therefore expected to be applicable to methods for treating diseases using artificial organs prepared using the aptamers according to the present invention.

The invention discloses a thymus decellularized scaffold as well as a preparation method, a kit and application thereof. The method comprises the following steps: 1) flushing thymus with a cleaning agent; 2) washing the cleaned thymus with a detergent A; 3) washing the washed thymus with a detergent B; 4) washing the washed thymus with a preservative; 5) cleaning the flushed thymus by using a cleaning agent; and 6) adding a preservative to prepare the thymus decellularized scaffold. The cleaning agent contains EDTA, antibiotics and PBS; the detergent A contains lauryl sodium sulfate and antibiotics; the detergent B contains anhydrous magnesium sulfate, anhydrous calcium chloride, Triton X-100 and antibiotics; and the preservative contains antibiotics and PBS. The method for amplifying thymus stromal cells in vitro based on the thymus decellularized scaffold is beneficial to directional induction of development and differentiation of hematopoietic stem progenitor cells into T cells, and has wide application potentials in large-scale culture of T cells in the future and in the field of T cell immunotherapy.

The present invention provides compositions and methods for producing insulin beta cells from pluripotent stem cells. The compositions and methods of the invention relate to stepwise differentiation when differentiated cells are cultured on lung tissue-derived decellularized scaffolds.