36 results about "Acellular scaffold" patented technology

A keratin hydrogel matrix serves as an effective acellular scaffold for axonal regeneration and facilitates functional nerve recovery.

The invention relates to a method for preparing the conspecific allo vessel de-cell support, which comprises that using the abdominal aorta of rabbit of New Zealand, using surface activator Triton X-100 to extract the liposome of cell membrane, and digesting the parenzyme, using nuclease DNAse and RNAse to degrade the DNA and RNA of cell, to obtain the support. The invention uses the similar structures of conspecific allo materials, with better histocompatibility, to support healing after surgery, with simple preparation and high efficiency. And the invention can be transplanted with low caused immunity reaction of host, to supply physical strength and tension. The invention can be used to replace blood vessel.

The invention discloses a construction method of a three-dimensional liver microtissue unit for a bio-artificial liver support system. The construction method is characterized by comprising the steps that decellularized scaffold components of a pig liver are encapsulated by microcapsules prepared from sodium alginate-polycations, liver cells, vascular endothelial cells and fibroblasts are composited, and the functional three-dimensional liver microtissue unit is constructed through cell self-assembly and applied to the bio-artificial liver support system. According to the method, operation is easy, cost is low, the liver cells, the vascular endothelial cells and the fibroblasts are self-assembled into three-dimensional liver microtissue by simulating interaction between the cells and an extracellular matrix, interaction between the cells and soluble factors and interaction between the cells and the cells in a liver tissue microenvironment, the three-dimensional liver microtissue has good synthesis, secretion and detoxification functions, and therefore the three-dimensional liver microtissue can serve as the microunit to be applied to the bio-artificial liver support system.

The invention provides a complex hernia patch and a preparing method and application thereof. The complex hernia patch comprises three layer structures which are an outer biological layer, an inner supporting layer and an outer biological layer from bottom to top respectively, wherein the outer biological layers are accellular stent layers, and the inner supporting layer is a polylactone mesh layer. The complex hernia patch prepared through the preparing method comprises the three layer structures, accellular stents in contact with tissue are located on the outer layers, cells can be induced to grow, and thus infection of a hernia coloboma part and formation of scars can be reduced; the polylactone mesh layer serves as the inner supporting layer so that the mechanical strength of the complex hernia patch can be maintained, the stability of the patch can be improved, and high abdomen expansion pressure can be borne; furthermore, the binding force of the inner layer and the outer layers is good, and thus the problems that due to the fact that the strength of an existing product is not stable, high abdomen expansion pressure cannot be borne, a stent is gradually degraded after cells grow into the stent, consequently tardive pain of hernia repair is brought, and relapse is likely to occur can be effectively solved; in addition, the preparing method is simple and capable of being applied to industrial production.

A composition of matter is provided comprising a devitalized, acellular tissue-derived scaffold seeded with differentiated cells, particularly pancreatic islet cells, wherein the cells can maintain cell-specific function or structure in culture on the scaffold. Methods of generating same and uses thereof are also provided.

The invention discloses a preparation method of acellular matrix hydrogel. The preparation method comprises the following steps: obtaining a tissue block; transferring the tissue block into a solutioncontaining 0.02% of pancreatin and 0.05% of ethylenediamine tetraacetic acid, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 3% of Triton X, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 4% of sodium deoxycholate, and performing stirring for 2-3 h; moving the tissue block into deionized water to be soaked for 12-18 h to obtain a decellularized scaffold; transferring the decellularized scaffold into 75% alcohol to be soaked for 30 min, transferring the decellularized scaffold into a solution containing 0.1% peracetic acid, and carrying out stirring for 2-3 h; freeze-drying the decellularized scaffold, grinding the decellularized scaffold into matrix powder, transferring the matrix powder into a solution containing pepsase hydrochloride, and performing stirring for digestion for 2-3 d to obtain pre-gel; and diluting the pre-gel to a preset concentration, and performing standing to obtain the acellular matrix hydrogel. The acellular matrix hydrogel is efficiently extracted from umbilical cord tissue, and the integrity of the internal structure of the acellular matrix is reserved to the maximum extent.

The invention provides a complex hernia patch and a preparing method and application thereof. The complex hernia patch comprises three layer structures which are an outer biological layer, an inner supporting layer and an outer biological layer from bottom to top respectively, wherein the outer biological layers are accellular stent layers, and the inner supporting layer is a polypropylene mesh layer. The complex hernia patch prepared through the preparing method comprises the three layer structures, accellular stents in contact with tissue are located on the outer layers, cells can be induced to grow, and thus infection of a hernia coloboma part and formation of scars can be reduced; the polypropylene mesh layer serves as the inner supporting layer so that the mechanical strength of the complex hernia patch can be maintained, the stability of the patch can be improved, and high abdomen expansion pressure can be borne; furthermore, the binding force of the inner layer and the outer layers is good, and thus the problems that due to the fact that the strength of an existing product is not stable, high abdomen expansion pressure cannot be borne, a stent is gradually degraded after cells grow into the stent, consequently tardive pain of hernia repair is brought and relapse is likely to occur can be effectively solved; in addition, the preparing method is simple and capable of being applied to industrial production.

The invention discloses an injectable decellularized scaffold for cartilage repair as well as a preparation method and application of the injectable decellularized scaffold, and relates to the technical field of medical materials. According to the method, a double-injection mode is adopted, and the gelated gradient injectable decellularized scaffold is obtained by injecting a pre-gel solution and a viscosity modifier simultaneously. The decellularized scaffold can load MSCs to be implanted into a body, is hydrolyzed under a physiological pH value, and slowly releases a medicine at an injured part, so that the differentiation of the loaded MSCs to cartilage cells is promoted, and the injured cartilage tissue is repaired; Due to the gradient property, original biological characteristics of the cartilage tissue can be better simulated, and a better repairing effect is achieved. According to the decellularized scaffold and the technology, the raw materials are cheap and wide in source, after clinical popularization, the pain of a patient can be remarkably relieved, the operation frequency is reduced, the treatment effect is improved, and remarkable social and economic benefits are achieved.

The invention discloses a tissue accellular solution. The tissue accellular solution contains lauryl sodium sulfate and ascorbic acid which are soluble in a buffer solution. The invention further discloses omentum majus tissue processed through the tissue accellular solution. Through antioxidation of the ascorbic acid (vitamin C), oxidative damage to acellular matrix in the accellular process is reduced, extracellular metrix protein in the acellular matrix is protected, and the biocompatibility of a decellularized scaffold is improved. The method has the advantages that operation is easy, cost is low, and the feasibility is high.

The present invention relates, in various embodiments, to methods of producing a tissue-mimetic construct having a basement membrane, methods of producing an acellular scaffold containing an extracellular matrix (ECM), methods of producing a scaffold comprising a hydrogel that is enriched in ECM components, methods of treating a condition in a subject in need thereof with a tissue-mimetic construct having a basement membrane, and methods of assessing whether an agent is suitable for administering to a tissue. The invention further relates to tissue-mimetic constructs and scaffolds produced in accordance with the methods of the invention.

The invention relates to a liver acellular stent construction method based on an irreversible electroporation technology. The method comprises the following steps of 1.1) weighing a rat, carrying outgas inhalation anesthesia by using isoflurane, maintaining the gas inhalation anesthesia, after the anesthesia is effective, disinfecting abdominal skin of the SD rat in a supine position by using 75%alcohol, and opening the abdomen layer by layer to expose the liver; 1.2) clamping a left lateral lobe area of the rat liver by using a caliper type electrode, and performing high-voltage pulsed electric field treatment; 1.3) after the irreversible electroporation treatment is finished, performing transhepatic portal vein catheterization, perfusing with heparinized PBS (Phosphate Buffer Solution), cutting off the subhepatic inferior vena cava until the liver is full and the color is gradually lightened, gradually dissociating tissues and ligaments around the liver, taking down the liver, andimmersing the liver into the heparinized PBS; 1.4) putting the taken liver into a clean container, and performing one-way perfusion with deionized water, and 1.5) after the deionized water perfusion is finished, performing one-way perfusion with PBS for 30 minutes to complete the preparation of the stent. By the method disclosed by the invention, the construction efficiency of the in-vitro acellular stent can be effectively improved under the condition of not applying harmful chemical preparations.

A method for effectively preparing a cornea acellular matrix tissue engineering scaffold by enzymatic method is as follows: (1) a porcine corneal matrix plate layer is put into diluted Myroilysin for decellularization, and flushed respectively with normal saline and distilled water to obtain a porcine corneal acellular scaffold board layer; (2) the porcine corneal acellular scaffold is put into a drill floor, a riboflavin solution is added dropwise, and the porcine corneal acellular scaffold is irradiated by an UV lamp, and then flushed respectively with the normal saline and the distilled water to obtain a riboflavin-crosslinked porcine corneal acellular scaffold; and (3) the riboflavin-crosslinked porcine corneal acellular scaffold obtained by the step (2) is dehydrated, sterilized and stored at 4 DEG C for standby use. The prepared acellular scaffold still retains enough mechanical strength, certain pores are formed, and after dehydration, the acellular scaffold has high transparency and is favorable for cell growth and migration.

The invention particularly relates to a tendon repair material, a preparation method and application in preparation of tendon repair products. The existing tendon repair material also has the defects that the induction regeneration effect is insufficient, the mechanical property of the repaired tendon tissue is poor, the ideal release effect is difficult to realize by adding an activity induction factor and the like. Aiming at the problems existing in the prior art, the periostin biologically-crosslinked tendon repair product is provided, periostin is promoted to be crosslinked with an acellular matrix and an acellular scaffold through addition of monoamine oxidase, so that a good slow-release effect is achieved, and after the periostin is implanted into a body, the periostin can be quickly released. The repair material can stimulate the activity of in-vivo amine oxidase, further improves the tendon repair effect, and has good industrialization and treatment prospects.

The invention discloses a decellularized scaffold and a preparation method thereof.The preparation method comprises the steps that decellularized bone powder is prepared, the decellularized bone powder, hydroxyl calcium polyphosphate and PLGA are mixed and then subjected to electrostatic spinning, and a carrier scaffold is obtained; soaking the carrier scaffold in a basic fibroblast growth factor solution to obtain a basic fibroblast growth factor-loaded carrier scaffold; and inoculating osteoblasts on the carrier scaffold loaded with the basic fibroblast growth factors to obtain the decellularized scaffold. According to the preparation method, cells and fat can be efficiently removed through a specific decellularization method, then decellularized bone particle powder, hydroxyl calcium polyphosphate and PLGA are subjected to electrostatic spinning to prepare the biological scaffold, the scaffold is made to have good biocompatibility, pore diameter structure and mechanical strength, and then the biological scaffold can be used for preparing the biological scaffold. And basic fibroblast growth factors and osteoblasts are loaded, so that proliferation and differentiation of the osteoblasts can be promoted, and an osteogenesis promoting effect is achieved.

The invention provides a preparation method of a tissue engineering liver based on a plant decellularized scaffold. The preparation method comprises the following steps: S1, cutting out a stem part of hollow oenanthe javanica; s2, immersing the stem part of the celery into a lauryl sodium sulfate solution, and then rinsing the stem part of the celery by utilizing trition x-100 containing hypochlorous acid, so as to obtain a decellularized scaffold; and S3, planting the hepatocytes on the acellular scaffold and culturing the hepatocytes.

The present invention provides a preparation method of a brain acellular stent. The preparation method is characterized by comprising the following steps: (1) adopting a detergent soaking rinsing method to obtain a brain acellular stent as a cell carrier; (2) prevascularizing the brain acellular stent in the step (1) to obtain a prevascularized brain acellular stent; and (3) transplanting PC12 cells into the prevascularized stent prepared in the step (2) and verifying formation of a vascular network in the prevascularized brain acellular stent and importance of seed cell transplantation in tissue engineering. The preparation method provides experimental basis for the brain acellular stent as the cell carrier for repairing central nervous system injury, and simultaneously, the preliminarilyconstructed brain acellular stent prevascularizing provides a new treatment strategy for transplanting the brain acellular stent-carrying seed cells after prevascularization to tissue and nerve regeneration and repair after traumatic brain injury.

The invention provides a method for preparing a cartilage tissue engineering scaffold from human umbilical cord Wharton jelly and the cartilage tissue engineering scaffold. The method for preparing the cartilage tissue engineering scaffold from the human umbilical cord Wharton jelly comprises the following steps: (1) collecting and pretreating of an umbilical cord specimen; (2) preparation of Wharton jelly tissue blocks; (3) freeze thawing of Wharton jelly tissue blocks; (4) chemical decellularization of Wharton jelly tissue blocks; and (5) freeze-drying of the Wharton jelly tissue blocks. The scaffold prepared by the invention can retain extracellular matrix components of Wharton jelly, especially related proteins for promoting cartilage differentiation and original structure characteristics, to the maximum extent, and at the same time effectively removes cell components. The Wharton jelly decellularized scaffold has the structural characteristics of being loose and porous, good in biocompatibility, conforming to the biodegradation law and highly similar to the cartilage extracellular matrix structure and component, and a new material system is provided for construction of cartilage tissue engineering scaffolds.

The invention relates to a tissue engineering patch and a preparation method thereof, and the preparation method comprises the following steps: (1) inoculating target cells on the surface of one sideof an acellular scaffold, placing a pyroptosis product on the other side of the acellular scaffold, and separating the pyroptosis product from the surface of the other side of the acellular scaffold;culturing the decellularized scaffold inoculated with the target cells and a pyroptosis product in a culture solution together; (2) when the target cells migrate from one side of the decellularized scaffold to the other side of the decellularized scaffold, removing the target cells which are not migrated on the surface of one side of the decellularized scaffold to obtain a tissue engineering patch; wherein the cell pyroptosis product is at least one of cell pyroptosis secreta, a cell pyroptosis extract and pyroptosis cells generated after cell pyroptosis. The tissue engineering patch preparedby the method has high cell loading capacity, target cells are uniformly distributed in the scaffold, and the tissue engineering patch has good biological activity.