a tissue decellularized fluid

A decellularization and tissue technology, applied in the field of tissue decellularization fluid for the preparation of acellular matrix, can solve the problems of destroying the components of omentum protein and affecting biological functions, so as to be beneficial to survival, reduce oxidative damage, and increase biological phase. capacitive effect

Active Publication Date: 2018-01-09
北京帝康医药投资管理有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the conventional decellularization treatment will generate a large amount of active oxygen, which will destroy the protein components in the acellular matrix of the omentum to a certain extent, and affect its biological function.

Method used

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Examples

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Embodiment 1

[0029] The preparation of embodiment 1 omentum decellularization liquid

[0030] Omentum tissue decellularization solution containing SDS and ascorbic acid was prepared, wherein the concentration of SDS was 1.5% by weight and volume ratio, and the concentration of ascorbic acid (vitamin C) was 0.2% by weight and volume ratio, prepared with sterile phosphate buffered saline (PBS).

Embodiment 2

[0031] Example 2 Decellularization of omentum derived from miniature pigs

[0032] Fresh minipig peritoneal omentum tissues (purchased from slaughterhouses) were taken and washed 3 times in PBS. After the degreasing treatment, soak it in 1L omentum decellularization solution for 18 hours, and wash it in PBS for 3 times, freeze-dry the omentum tissue treated with the decellularization solution, sterilize it by irradiation, and store it at room temperature .

Embodiment 3

[0033] Example 3 Immunohistochemical staining of omentum acellular matrix

[0034] The omentum acellular matrix prepared in Example 2 was fixed with 4% paraformaldehyde solution, dehydrated with concentration gradient alcohol, embedded in paraffin, and slices with a thickness of 4 μm were prepared. The slices were dried on a pathological tissue drying apparatus for 2 hours. Subsequently, the tissue sections were hydrated with graded alcohol (from high to low concentration), and the sections were rinsed with distilled water. Add 3% hydrogen peroxide for 10 minutes to remove endogenous peroxidase; rinse three times with PBS, 5 minutes each time; use antigen retrieval solution for microwave repair, cool to room temperature after repair, rinse three times with PBS, 5 minutes each time; 1% bovine serum white Proteins were blocked at 37°C for 30 min; anti-Collagen I (1:200) and anti-Collagen IV (1:200) primary antibodies were added dropwise respectively, and overnight at 4°C. Wash...

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Abstract

The invention discloses a tissue accellular solution. The tissue accellular solution contains lauryl sodium sulfate and ascorbic acid which are soluble in a buffer solution. The invention further discloses omentum majus tissue processed through the tissue accellular solution. Through antioxidation of the ascorbic acid (vitamin C), oxidative damage to acellular matrix in the accellular process is reduced, extracellular metrix protein in the acellular matrix is protected, and the biocompatibility of a decellularized scaffold is improved. The method has the advantages that operation is easy, cost is low, and the feasibility is high.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a tissue acellular liquid for preparing an acellular matrix. Background technique [0002] Decellularization technology mainly refers to the use of decellularization technology (physical, chemical, enzymatic and other methods) to remove various cellular components and genetic materials in tissues to obtain natural biological scaffold materials. Because it retains the three-dimensional structure and natural components of the extracellular matrix, it is conducive to cell adhesion, proliferation, differentiation and biological functions. Therefore, it has incomparable advantages in tissue repair and regeneration with other scaffold materials. At present, people have been able to obtain acellular matrix scaffold materials from a variety of tissues, such as small intestine, skin, blood vessels, cornea and more complex heart, lung, liver, kidney and so on. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 饶义伟蓝德宾
Owner 北京帝康医药投资管理有限公司
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