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Decellularized scaffold and preparation method thereof

A decellularized scaffold and decellularized bone technology, applied in the field of biomaterials, can solve problems such as pain and fractures, donor site infection, infection, etc., and achieve the effect of promoting value-added and differentiation, and good biocompatibility

Pending Publication Date: 2022-08-09
万绵水
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both of these two treatment methods have certain shortcomings. The sources of autologous bone grafting are very limited, and there are complications such as infection, pain, and fracture at the donor site; allograft bone has risks such as infection, disease transmission, and immune rejection, and there are also risks. ethical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment is a preparation method of acellular scaffold, and the preparation method includes the following steps:

[0032] (a) take a fresh pig femur, remove the surrounding tissue, then wash, and then pulverize to a particle size of not more than 5 mm to obtain bone particles;

[0033] (b) placing the bone particles in a chloroform methanol solution and stirring for 6h, filtering, collecting the bone particles and precipitation therein, and the volume ratio of chloroform and methanol is 2:1;

[0034] (c) Under stirring conditions, the bone precipitate was placed in the ammonia water eluent containing SDS for elution for 25 days, the eluent was replaced every 30h, and after elution was completed, washing and freeze-drying were performed to obtain decellularized cells. Bone particle precipitation, wherein the concentration of SDS in the eluate is 0.8%, and the concentration of ammonia water is 0.2%;

[0035] (d) grinding the precipitation of the decellularized bon...

Embodiment 2

[0040] This embodiment is a preparation method of acellular scaffold, and the preparation method includes the following steps:

[0041] (a) take a fresh pig femur, remove the surrounding tissue, then wash, and then pulverize to a particle size of not more than 5 mm to obtain bone particles;

[0042] (b) placing the bone particles in a chloroform methanol solution, stirring for 4-6 hours, filtering, collecting the bone particles and precipitation therein, and the volume ratio of chloroform and methanol is 6:1;

[0043] (c) Under stirring conditions, the bone precipitate was placed in the ammonia water eluent containing SDS for elution for 15 days, the eluent was replaced every 24 hours, and after elution was completed, washing and freeze drying were performed to obtain decellularized cells. Bone particle precipitation, wherein the concentration of SDS in the eluate is 2%, and the concentration of ammonia water is 0.1%;

[0044] (d) grinding the precipitation of the decellulari...

Embodiment 3

[0049] This embodiment is a preparation method of acellular scaffold, and the preparation method includes the following steps:

[0050] (a) take a fresh pig femur, remove the surrounding tissue, then wash, and then pulverize to a particle size of not more than 5 mm to obtain bone particles;

[0051] (b) placing the bone particles in a chloroform-methanol solution, stirring for 5h, filtering, collecting the bone particles and precipitation therein, and the volume ratio of chloroform and methanol is 4:1;

[0052] (c) Under stirring conditions, the bone precipitate was placed in the ammonia water elution solution containing SDS for elution for 20 days, and the eluent was replaced every 25 h. After the elution was completed, washing and freeze-drying were performed to obtain decellularized cells. Bone particle precipitation, wherein the concentration of SDS in the eluate is 1%, and the concentration of ammonia water is 0.15%;

[0053] (d) grinding the precipitation of the decellu...

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Abstract

The invention discloses a decellularized scaffold and a preparation method thereof.The preparation method comprises the steps that decellularized bone powder is prepared, the decellularized bone powder, hydroxyl calcium polyphosphate and PLGA are mixed and then subjected to electrostatic spinning, and a carrier scaffold is obtained; soaking the carrier scaffold in a basic fibroblast growth factor solution to obtain a basic fibroblast growth factor-loaded carrier scaffold; and inoculating osteoblasts on the carrier scaffold loaded with the basic fibroblast growth factors to obtain the decellularized scaffold. According to the preparation method, cells and fat can be efficiently removed through a specific decellularization method, then decellularized bone particle powder, hydroxyl calcium polyphosphate and PLGA are subjected to electrostatic spinning to prepare the biological scaffold, the scaffold is made to have good biocompatibility, pore diameter structure and mechanical strength, and then the biological scaffold can be used for preparing the biological scaffold. And basic fibroblast growth factors and osteoblasts are loaded, so that proliferation and differentiation of the osteoblasts can be promoted, and an osteogenesis promoting effect is achieved.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to an acellular scaffold and a preparation method thereof. Background technique [0002] Bone defects caused by trauma, tumors, infections and congenital factors have always been a difficult problem to solve in clinical practice. At present, autologous bone and allogeneic bone transplantation are mainly used in clinical treatment of bone defects. However, both of these two treatment methods have certain shortcomings. The source of autologous bone transplantation is very limited, and there are complications such as infection, pain and fracture in the donor site. In addition to the risks of infection, transmission of diseases and immune rejection, allogeneic bone still exists ethical issues. Therefore, clinicians have been working on developing a bone graft scaffold with stable structure and performance, and osteoconductive and osteoinductive effects. [0003] In view o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/18A61L27/54A61L27/38A61L27/56A61L27/50
CPCA61L27/3633A61L27/3608A61L27/365A61L27/3691A61L27/3687A61L27/12A61L27/18A61L27/54A61L27/3821A61L27/3847A61L27/56A61L27/50A61L2430/02A61L2300/414A61L2430/40C08L67/04
Inventor 万绵水
Owner 万绵水
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