18 results about "Ossification" patented technology

The application relates to the technical fields of medical image processing and artificial intelligence, and provides a bone age evaluation method and system based on an AI model and a bone age instrument. The left wrist X-ray image of a subject detected in the bone age instrument is acquired, and the demographic information of the subject is recorded. The detected left wrist X-ray image is pretreated through gray standardization, noise filtering and a self-supervised learning model. The Vision Transformer is used to extract the distal radius epiphysis and metaphysis width ratio, the third metacarpal metaphysis length and the auxiliary ossification point maturity grading skeletal features, and the multi-modal data fusion is carried out with the demographic information. The multi-layer perception model is used to predict the fused multi-dimensional bone age related data, and a bone age evaluation report is output, so that the error in the bone age prediction of children and adolescents is effectively reduced, and the detection efficiency is improved.

The invention discloses an application of a JAM3 modified engineered vesicle in preparation of a medicine for treating bone injury, the JAM3 modified engineered vesicle is prepared, and the osteogenesis promoting effect of the JAM3 modified engineered vesicle on bone marrow mesenchymal stem cells (BMSCs) is proved through ALP staining, RT-qPCR, WB and other experiments at the cellular level, so that the osteogenesis promoting effect of the JAM3 modified engineered vesicle on the bone marrow mesenchymal stem cells (BMSCs) is improved, and the osteogenesis promoting effect of the JAM3 modified engineered vesicle on the bone marrow mesenchymal stem cells (BMSCs) is improved. Meanwhile, the effect of JAM3 modified engineered vesicles on promoting osteogenesis of bone defect mice is defined through in-vivo experiments. According to the invention, osteogenesis direction differentiation and bone formation capability of BMSC can be promoted, and bone formation capability of bone defect mice can be promoted in vivo; the compound can be used for treating related diseases such as bone injury.

The invention relates to the technical field of bone repair materials, in particular to a horse antler powder support material preparation device and system, and the device comprises an ossification degree pretreatment unit, a crushing unit, a mixing unit, a printing unit and a regulation and control unit; the ossification degree pretreatment unit comprises a transmission table and a controller, a positioning conveying assembly is arranged on the surface of a transmission part of the transmission table, and a detection assembly and a cutting assembly are arranged on a support part of the transmission table; the preparation system comprises an ossification degree pretreatment module, an air pressure crushing module, a proportioning and mixing module, a 3D printing module and a degradation rate regulation and control module. According to the method, ossification degree grading pretreatment is carried out on the raw material antler, precise recognition and directional cutting are carried out on a cancellous bone region, a compact bone mass core is removed, and the problems of active ingredient fluctuation and non-uniform pore structure caused by individual difference of ossification degrees are solved.

The application discloses a traditional Chinese medicine active activated bone growth type support implant material and a preparation method thereof, and relates to the technical field of medical materials.The traditional Chinese medicine active activated bone growth type support implant material is composed of an organic-inorganic composite porous antibacterial support and a medicinal efficacy filler.Co hollow spheres loaded with carbon nanotubes, sodium alginate, nano-hydroxyapatite and transition metal carbide nanosheets are cross-linked and compounded to form the organic-inorganic composite porous antibacterial support, which not only has good mechanical properties and antibacterial properties, but also can stably bear more medicinal efficacy fillers and slowly release the medicinal efficacy fillers, and the medicinal efficacy fillers further improve the stability of the support structure.The synergistic effect of the two can significantly enhance the antibacterial property and osteogenesis of the implant material, and excellent bone repair effect is achieved.

The application discloses an oral implant with a surface provided with an osteogenesis-promoting and antibacterial coating and a preparation method of the oral implant, wherein the coating comprises chitosan and an antibacterial osteogenesis-promoting fusion polypeptide, and the amino acid sequence of the chitosan and the antibacterial osteogenesis-promoting fusion polypeptide is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The application further provides a method for preparing the oral implant by using the coating of the implant. The oral implant has obvious bacteriostatic and osteogenesis-promoting effects, has good biocompatibility, and has a wide application prospect in the field of oral implantation.

The application discloses a piezoelectric porous scaffold with high efficient osteogenesis and a preparation method thereof. The piezoelectric porous scaffold is obtained by mixing a biological component and a piezoelectric component through an adhesive, sintering and then performing polarization treatment. The piezoelectric porous scaffold can efficiently load and adsorb bioactive protein factors, realizes effective release of the protein, and has piezoelectric performance and osteogenesis activity. Under the multiple effects of the piezoelectric effect, the porous structure and the bioactivity of the protein of the scaffold, the piezoelectric porous scaffold can quickly start early response of bone marrow stromal cells in a synergistic manner, and then promote cell adhesion, proliferation and osteogenic differentiation. The piezoelectric porous scaffold has important significance for efficient and rapid repair of large bone defects, bone nonunion and other bone defects.

The application discloses a preparation method and application of an injectable high-strength bone tissue adhesive, and belongs to the technical field of biomaterials. The hydrogel tissue adhesive comprises micron or nanometer organic particles carrying amino groups, water-soluble polymer which can be chemically crosslinked with the amino groups, and micron or nanometer inorganic particles which can promote osteogenesis; under the action of water, the hydrogel tissue adhesive can form a continuous and porous particle network, and the chemical reaction between the particles and the polymer can provide good adhesive performance, and the adhesive strength can reach 5Mpa. In addition, the non-covalent interaction between the particles can make the material better stay in the defect site, and realize the filling of the bone defect. The inorganic component in the material component of the application can release ions which can promote osteogenesis, and promote the repair of the bone defect. In addition, the hydrogel tissue adhesive can also be used as a bone wound hemostatic material and a cell scaffold material, and has a wide application prospect in the field of bone repair.

The invention belongs to the field of tissue regeneration materials, and relates to a 3D printing triboelectric material and application thereof in bone and cartilage defect repair. The material can be used as 3D printing ink for manufacturing a triboelectric bracket. The scaffold is prepared by taking biodegradable PGS as a matrix, PEDOT: PSS as a conductive component and sodium chloride as a pore-forming agent through combination of 3D printing and a salt particle pore-forming method, and has a multi-stage porous structure and excellent triboelectric properties. The wire diameter, interlayer spacing and micropore distribution of the stent are accurately regulated and controlled through the 3D printing technology, so that in-situ self-energized electrical stimulation of the stent is realized in an in-vivo dynamic mechanical environment; the scaffold can promote the BMSCs to differentiate into cartilage cells, construct tissue engineering cartilage and accelerate the process of ossification in cartilage, finally realizes efficient repair of bone and cartilage defects, is excellent in biocompatibility and biodegradable, does not need an external power supply device, and has a wide clinical transformation prospect in the field of bone and cartilage defect repair.

To provide methods for treating disease and disorders associated with pathological calcification or pathological ossification.SOLUTION: The method comprises administering a composition comprising at least one agent selected from the group consisting of Ecto-Nucleotide pyrophosphate / Phosphodiesterase-1 (NPP1) polypeptide and a fragment, derivative, mutant or mutant fragment thereof, and an activator of NPP1 polypeptide and a fragment, mutant or mutant fragment thereof. Preferably, the NPP1 polypeptide or a fragment, mutant or mutant fragment thereof is a soluble recombinant NPP1 polypeptide or a fragment, mutant or mutant fragment thereof.SELECTED DRAWING: Figure 1

The invention discloses a customized tibia prosthesis, and relates to the technical field of skeleton prostheses, the customized tibia prosthesis comprises a tibia prosthesis main body, the two opposite ends of the tibia prosthesis main body are both provided with positioning bosses, the section of each positioning boss is the same as the shape of a marrow cavity of a tibia backbone, and the positioning bosses are used for being embedded into the marrow cavity of the tibia backbone; the tibia artificial bone body further comprises a pore support and a solid support, the pore support is of a porous structure, and the solid support is fixedly connected with the pore support. And the dense pore structures of the positioning boss and the pore bracket are beneficial to promoting the formation of new bone tissues, so that the bone tissues can grow in each pore, and the tibia prosthesis has enough mechanical stability and long-term ossification support. The bone prosthesis has the technical effect of improving the adaptive positioning performance and stability of the bone prosthesis and the original bone.

Aspects of the disclosure relate to compositions and methods for modulating bone growth, for example by increasing osteogenesis and / or decreasing osteoclastogenesis. The disclosure is based, in part, on recombinant adeno-associated virus (rAAV) vectors encoding microRNAs or miRNA inhibitors that inhibit endogenous miR-214-3p and / or mediate overexpression of miR-34a-5p in osteoblasts and osteoclasts. In some embodiments, compositions described by the disclosure are useful for treating certain bone diseases or disorders, such as osteoporosis.

The invention discloses a preparation method of a double-drug-loading antibacterial bone repair scaffold capable of inducing bone and blood vessel generation, and aims to provide a preparation method of a novel 3D printing multifunctional sodium alginate scaffold. The preparation method is characterized in that copper peptide molecules are grafted on the surfaces of the dopamine surface modified cellulose acetate microspheres through an EDC cross-linking effect, so that effective loading of copper peptide drugs is realized. The obtained cellulose acetate drug-loaded microspheres are dispersed in a sodium alginate solution for low-temperature 3D printing, and the printed stent is subjected to in-situ grafting with an alendronate sodium drug under the action of coordinate bonds in the Zn < 2 + > crosslinking process to obtain the double-drug-loaded stent. The preparation method has the characteristics that the prepared 3D printing double-drug-loading sodium alginate stent embedded with the cellulose acetate drug-loading microspheres can slowly release copper peptide, alendronate sodium drugs and Zn < 2 + > in a lasting manner, and is a multifunctional bone repair stent which has enhanced biological activity, mechanical properties and osteogenesis and excellent angiogenesis promoting and anti-infection capabilities at the same time.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a composition with an osteogenesis effect, which is prepared from the following raw materials in parts by weight: 15-45 parts of magnetic nanoparticles and 2-10 parts of aspartatide. According to the invention, the magnetic nanoparticles and the aspartatide are combined, and the nanoparticles magnetized in a magnetic field are coupled with the aspartatide, so that thickening of local alveolar bone can be promoted, and an excellent alveolar bone osteogenesis effect is achieved. The magnetic nanoparticles and the aspartatide are coupled and then act on the alveolar bone through hydrogel wrapping, the slow release effect can be achieved, the concentration of active ingredients in a treatment window is maintained, the curative effect is improved, the unstable curative effect caused by medicine concentration fluctuation is reduced, meanwhile, the administration frequency is reduced, the medication compliance of a patient is improved, and the treatment effect of the alveolar bone is improved. The practical popularization and application value is realized.

The present invention relates to a novel lappaconitine derivative and a pharmaceutically acceptable salt thereof, a preparation method therefor, and a medical use thereof using osteogenesis-promoting activity. The lappaconitine derivative induces the differentiation of stem cells into preosteoblasts, and increases bone density when administered to an osteoporosis animal model, and induces osteogenesis, and thus can be effectively used for preventing, alleviating, or treating bone-related diseases such as osteoporosis.

This invention discloses a guidewire-connected negative electrode spinal fusion device system, comprising a fusion device body for mounting between the upper and lower vertebrae, supporting the upper and lower vertebrae and providing bone graft space; a screw-rod fixation mechanism for connecting to and fixing the upper and lower vertebrae on their sides; a positive electrode component for generating positive charge and located outside the bone graft space within the screw-rod fixation mechanism; a guidewire, with its two ends electrically connected to the fusion device body and the positive electrode component respectively; and a guidewire sleeve fitted over the guidewire. The system of this invention places the positive electrode component on the top wire and connects it to the fusion device body via the guidewire. The positive electrode component carries a negative charge on its surface, allowing it to directly contact the upper and lower vertebrae, effectively attracting calcium and phosphate ion deposition, activating osteoblast proliferation and differentiation, accelerating bone ingrowth and fusion, and promoting osteogenic growth. By placing the positive electrode component outside the bone graft space and not contacting the bone graft area, the problem of positive charge inhibiting osteogenic growth near the bone graft area is solved.

The application discloses a method for screening sika deer bone peptide segments and application of the peptide segments, and belongs to the technical field of peptide screening and application. According to the screening method, sika deer cancellous bone samples in five different development periods, i.e., an initial stage of tray shedding, a two-culm early stage, a two-culm stage, a four-culm stage and a complete ossification stage, are collected respectively; the sika deer cancellous bone samples in the five different development periods are mechanically broken respectively, protein extraction is carried out by using a lysis buffer, the extracted protein samples in the five different development periods are subjected to enzyme cutting treatment respectively, five peptide segment samples in the five different development periods are obtained, liquid chromatography-mass spectrometry is used for peptide segment separation and identification, six common difference peptide segments in comparative development periods are obtained, and specific difference peptide segments conforming to the periodic osteoporosis phenomenon of sika deer are screened out. According to the screening method, sika deer bone active peptides for treating osteoporosis can be obtained, and bone formation can be promoted and bone resorption can be inhibited.

The application provides application of a composition in preparation of a bone repair material, wherein the composition comprises, based on the total dry matter of the composition: 70-90% of coral hydroxyapatite particles, 5-30% of recombinant human collagen, and 0-7% of excipients, and the bone repair material is a cancellous bone-like material with a porosity of 85-99%. The composition has high porosity and a longitudinal gradient structure, can simulate the characteristics of natural bone tissue, has excellent biological compatibility, biodegradability, hydrophilicity, osteogenic performance and shape memory function, has excellent clinical use convenience, hemostatic and healing promotion effects, excellent osteogenesis, and has a wide application prospect in preparation of the bone repair material for filling and / or repairing bone defects.

The invention belongs to the technical field of biological medicine, and particularly relates to a biological switchable siRNA delivery composite material based on RNAi and a preparation method and application thereof. The invention designs a novel biological switchable siRNA delivery system (BiRDS) based on RNAi (Ribonucleic Acid Interference) by combining good endocytosis performance and stability of tetrahedral framework nucleic acid and gene targeting specificity of siRNA, the system consists of three siRNAs (three magic birds) and a nucleic acid core (central sun), and by loading small interfering RNA to target casein kinase 2 interacting protein 1 (siCkip-1), the novel biological switchable siRNA delivery system (BiRDS) based on RNAi is obtained. The siRNA is specifically delivered to the bone formation surface, the loading capacity is improved to the maximum extent, the kip gene is efficiently silenced, cell proliferation and migration are promoted, the good osteogenesis induction effect is achieved, and a new thought is provided for treatment of maxillofacial bone defects.