The invention discloses a pilose antler moisturizing mask and a preparation method thereof. The pilose antler moisturizing mask is prepared from the following raw materials: butanediol, trehalose, 1,2-hexanediol, hydrolyzed royal jelly protein, a helichrysum italicum extract, p-hydroxyacetophenone, a ganoderma sinensis extract, bis-diethoxy diethylene glycol cyclohexane 1,4-dicarboxylic ester, phenoxyethanol, an acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, sclerotium gum, arginine, PEG-60 hydrogenated castor oil, lactobacillus/ginseng root fermentation product filtrate, ethylhexylglycerin, methylparaben, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sodium hyaluronate, sodium hyaluronate, a wild soybean germ extract, hydrogenated lecithin, lecithin, fitosterole, tocopherol, essence, a pilose antler extract, glycerol and water. By adopting plant essence such as the pilose antler extract, ginseng and ganoderma sinensis, the skin is nourished and tender, and water replenishment and moisturizing are achieved. The wild soybean germ extract, the lecithin and the like are matched, so that moisture is firmly locked while the skin is nourished.