20 results about "Heme iron" patented technology

The present application belongs to the field of biological medicine and molecular biology technology, and particularly relates to a marker for predicting the occurrence of repeated implantation failure and application thereof. The present application finds that abnormal lipid peroxidation in uterine epithelium can cause implantation defects, and the lipid peroxidation level of the uterus in patients with repeated implantation failure or repeated spontaneous abortion is enhanced, wherein the content of active aldehyde substance MDA and non-heme iron in serum can be used as a new non-invasive clinical index for predicting RIF patients; further, eliminating uterine lipid ROS to improve endometrial receptivity can be used as a potential new treatment strategy to improve embryo implantation and clinical pregnancy rate, and therefore has good practical application value.

This invention provides a high-recovery heme iron extraction method and apparatus using enzymatic hydrolysis-multi-stage centrifugal coupling, relating to the fields of biopharmaceuticals and fluid dynamics processing technology. The method first constructs a polyanionic electrolyte shielding system, utilizing electrostatic potential energy to induce conformational instability of globin and pre-exposure of hydrophobic cavities, reducing binding energy at the molecular level and solving the problem of heme's encapsulation resistance in high-concentration systems. Then, high-shear coupled enzymatic hydrolysis is performed within a micron-sized liquid film generated by a hypergravity field, utilizing the instantaneous synergy of mechanical shear stress and enzymatic degradation to achieve directional exfoliation of heme, eliminating secondary re-adhesion effects. Finally, directional phase transition regulation is performed based on density gradient drive, and a high-frequency pulsed pressure field is introduced for in-situ exchange washing, distinguishing and retaining impurities and crystal cores, outputting a high-purity, flavor-neutral heme iron product. This invention effectively improves the controllability of the extraction process and the stability of industrial continuous production.

The application relates to the technical field of meat processing, in particular to a vacuum circulation pickling method for meat. The method comprises the following steps: submerging meat pieces to be pickled in pickling liquid added with 0.1% hematin iron; first, applying a transient high-voltage electromagnetic pulse to precisely perforate the cell membrane of the meat; then, applying an alternating vortex magnetic field with a specific frequency; utilizing the high-frequency micro-vibration of the hematin iron in the magnetic field to generate a magnetohydrodynamic effect, expand the micro-pore channel and pump the pickling material molecules into the cell interior; finally, combining vacuum circulation alternating treatment to further press the pickling material into the tissue gap. The added hematin iron significantly enhances the promotion effect of the magnetic field on the pickling material delivery, and the pickling time is shortened to 30 minutes or less. It is proved through measurement that the pickling liquid absorption rate of the process is high, the penetration reaches the geometric center of the meat pieces, and the integrity and water holding capacity of the meat structure are perfectly preserved.

The application provides a low-temperature vacuum-multicavity evaporation linkage heme iron preparation method and device, relates to the technical field of biological medicine and functional food deep processing, and solves the problems of local overheating and fouling caused by traditional wall heat transfer.

This invention relates to the technical field of pet food, and more particularly to a functional pet nutritional supplement and pet nutritional food and its preparation method, comprising 10%–15% beef liver chunks, 20%–30% chicken breast strips, 35%–45% a mixture of cooked rice flour / corn / rice, 0.3%–1.0% chicken oil, 0.3%–1.0% fish oil, 2%–5% pumpkin puree, 1%–3% chopped carrots, 3%–7% egg yolk powder, 3%–7% a mixture of traditional Chinese medicine extracts, 0.5%–1.5% amino acid and mineral premix, 0.5%–1.5% vitamin and antioxidant premix, 0.0001%–0.001% probiotic mixture, 0.05%–0.3% potassium sorbate, 0.05%–0.3% sodium benzoate, 0.05%–0.2% citric acid, and BHA / BHT. 0.05%–0.2%, with the remainder being chicken broth. Beef liver is rich in heme iron, which, combined with lecithin in egg yolk powder, promotes iron absorption. Traditional Chinese medicine extracts significantly reduce IL-6, IgG, and IgM levels, regulating hyperimmune states. Meanwhile, ingredients such as astragalus enhance normal immunity, achieving a balance of "strengthening the body's resistance and eliminating pathogens."

The invention discloses application of an iron-containing substance. The invention finds that the iron-containing substance can be used for preparing the medicine for treating leucopenia and / or thrombocytopenia; the iron-containing substance is selected from myoglobin and a derivative thereof, hemoglobin and a derivative thereof, ferrous sulfate, ferrous fumarate, ferrous gluconate, a polyferose compound, ferrous succinate, iron amino acid chelate, heme, heme iron polypeptide, ferric hydroxide polymaltose, ferrous lactate, ferric pyrophosphate and iron dextran; and one or more of iron hydroxymaltose. The iron-containing substance can effectively increase the number of leukocytes and / or platelets.

The present invention discloses a heme iron – glycine chelated iron supplement preparation, belonging to the technical field of nutritional and pharmaceutical preparations. The preparation employs a four-source composite of heme iron, glycine chelated iron, ferrous gluconate, and ferrous lactate, enabling iron supplementation via heme receptor absorption, amino acid chelation pathways, and ionic diffusion. This design overcomes the limitations of single iron sources, including gastrointestinal irritation and low absorption of inorganic iron, dietary inhibition of conventional organic iron, and the high cost or slow onset of novel iron sources. The four iron sources act synergistically to reduce free iron ions and integrate multiple absorption pathways. As a result, gastrointestinal irritation is minimized, and the inhibitory effects of phytic acid, oxalic acid, and tannins are reduced, enabling stable and efficient intestinal absorption. The preparation achieves non-irritating, highly bioavailable, and sustained iron supplementation.

1. Name of the designed product: packing box (curved baby hematin iron red ginseng plant beverage). 2. Use of the designed product: packing for solid beverage. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view.

The invention discloses ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and a preparation method thereof, and belongs to the technical field of milk powder, the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder comprises the following components in parts by mass: a wet process part: 420-455 parts of whole milk powder, 306-345 parts of dried skim milk, 185-205 parts of isomaltooligosaccharide (type 50) powder, 35-40 parts of polydextrose, 11.5-14.5 parts of a milk powder nutrition enhancer III, 0.3-0.5 part of compound L-carnitine, and 0.1-0.3 part of heme chloride; according to the ruddy heme iron red ginseng and donkey-hide gelatin nutritional milk powder and the preparation method thereof, the heme chloride is adopted as an iron source, the bioavailability of the red heme iron red ginseng and donkey-hide gelatin nutritional milk powder is remarkably higher than that of ferrous sulfate used in traditional iron-supplementing milk powder, meanwhile, through beta-cyclodextrin embedding treatment, the fishy smell problem of an iron agent is solved, gastrointestinal tract stimulation is avoided, and L-sodium ascorbate is matched, so that the nutrition of the milk powder is improved. And the iron absorption rate can be further improved.

The invention relates to a mononuclear non-heme iron enzyme catalytic product indole compound and a preparation method thereof, and relates to the technical field of enzyme engineering. According to the method, a natural substrate is obtained through fermentation of a bean dreg culture medium, target protein is induced, expressed and purified by using an escherichia coli chassis, and then large-scale biochemical reaction of the protein, the substrate and cofactors is carried out in vitro. The compound is prepared by combining modern chromatographic separation and purification technologies such as analytical, semi-preparative and preparative high performance liquid chromatographs, medium-pressure liquid chromatographs and the like. The structure is determined to be a novel enzymatic product of OkaE in-vitro catalysis by adopting a spectroscopy structure identification method. According to the invention, the molecular structure of okaramine is modified by adopting an enzymatic modification strategy, an important way is provided for widening the structural diversity of okaramine alkaloids, and the method has remarkable scientific significance and application value.

The invention relates to the technical field of biological medicine, in particular to an ovary protection oral liquid containing heme iron composite peptide and a preparation method and application thereof. The oral liquid comprises the following core components: 0.05-0.2 part of heme iron, 1.0-4.0 parts of peptide components, and preferably 0.5-2 parts of natto powder, it is found and proved for the first time that the heme iron and the specific peptide components have a synergistic effect on improvement of the ovarian function, ovarian oxidative damage can be remarkably reduced, the ovarian hypoxia state can be improved, and the oral liquid can be used for treating ovarian diseases. The ovarian blood perfusion amount and the uterine ovarian artery blood flow speed are increased. Meanwhile, the natto powder and the carob bean extract are added to generate a dual synergistic effect with the core component, the stability and bioavailability of the active components are ensured through an optimized preparation process, and animal experiments prove that the oral liquid can comprehensively and effectively protect the ovarian function and improve the ovarian function. The invention provides a safe and effective solution for solving the problems of single effect and unobvious synergistic effect of oral products in the prior art.

The application discloses a kind of tonifying qi and nourishing blood compositions, belong to food field.The composition provided in the application includes plant fermentation product, heme iron, folic acid, ascorbic acid, wherein plant fermentation product is made of angelica sinensis powder, ginseng powder, red date powder, red bean powder, red skin peanut powder, wolfberry powder, is filtered after fermentation by aspergillus oryzae, lactobacillus crispatus, sterilized, freeze-dried and prepared.The multi-strain mixed fermentation process is used in the application, and the synergistic effect between different strains can effectively improve the qi and blood nourishing effect of the composition.

This invention provides a method and apparatus for extracting heme iron based on a dual-membrane cascade and oxygen isolation protection, belonging to the field of biochemical separation and purification technology. The method first constructs a pneumatically suspended hemolytic environment, utilizing a metal sintered microporous distributor to generate a micron-sized inert bubble fluidized bed, achieving red blood cell disruption and deep deoxygenation under zero mechanical shear conditions, solving the molecular damage and oxidation problems caused by traditional processes. Then, a gas-liquid co-flow ultrafiltration mechanism is employed, introducing pulsed plug flow drive to physically peel off the protein gel layer on the membrane surface online, achieving dynamic anti-clogging and long-term stability in the macromolecular separation process. Finally, transmembrane partial pressure extraction is performed based on a reverse gas-scan nanofiltration model, distinguishing the migration paths of water molecules and dissolved oxygen, outputting a highly active concentrate and a closed-loop circulating gas flow. This invention effectively improves the extraction efficiency of the product Fe. 2+ Retention rate, purity, and continuity of industrial production.

The invention belongs to the field of feed additives, and particularly relates to a compound feed additive for preventing verruca of goose soles and a goose feed thereof. The compound feed additive can be directly added into growth fattening feed of geese, and the health of goose foot pads is improved through the synergistic effect of multiple components. In the formula, the wheat bran / rice bran promotes generation of intestinal short-chain fatty acid through dietary fibers and enhances synthesis of a biological membrane of an epidermal structure; the garlic powder provides sulfur-containing amino acid and strengthens a keratin cross-linked structure; linoleic acid in DDGS reduces the level of foot inflammatory factors by regulating and controlling metabolism of arachidonic acid; the vitamin B group maintains epidermal differentiation, and the vitamin E / K3 repairs the lipid barrier; the blood powder provides high digestible protein and heme iron, and tissue regeneration is accelerated; zinc sulfate and yeast selenium cooperate to remove oxidation products and inhibit cutin abnormal hyperplasia. According to the scheme, through triple mechanisms of nutrient enrichment, inflammation inhibition and oxidation balance, the occurrence rate of verrucosis of the foot pad is effectively reduced, and meanwhile, the drug residue risk and the physical protection cost are avoided.

The invention provides a compound dietary nutrient for tonifying qi and blood and a preparation method thereof, and relates to the field of health food. The invention relates to a compound dietary nutrient for tonifying qi and blood and a preparation method thereof. The compound dietary nutrient capable of tonifying qi and blood is prepared from the following raw materials in percentage by mass: 23.9% of plant extract, 22.334% of maltodextrin, 10% of vitamin C, 8% of ferrous glycinate, 6% of fish collagen peptide, 3% of sodium hyaluronate, 1.8% of zinc glycinate, 1% of heme iron, 0.06% of copper gluconate, 0.6% of 2-oxoglutaric acid, 0.2% of vitamin B2, 0.006% of vitamin B12, 0.2% of vitamin B6, 1.4% of vitamin E and the balance of water. 0.3% of pyrroloquinoline quinone disodium salt, 1.2% of silicon dioxide and 20% of gelatin capsules. Through mutual cooperation of the components, the nutrient elements can promote qi and blood of a human body.

This invention provides a mutant of heme iron cytochrome P450 enzyme with enhanced electrocatalytic rate and its application. By performing site-directed mutagenesis on the heme iron cytochrome enzyme with the amino acid sequence shown in SEQ ID NO:1 as the parent, leucine at position 82 of SEQ ID NO:1 is mutated to glutamine, phenylalanine at position 167 is mutated to methionine, or valine at position 280 is mutated to threonine, a mutant with enhanced electrocatalytic rate is obtained, thereby solving the technical problem of low electrocatalytic rate of heme iron cytochrome enzyme in the prior art.

The application discloses a method for extracting and detecting heme iron in pilose antler and belongs to the technical field of food and Chinese medicinal material detection. In view of the problems that heme iron in pilose antler is easy to attenuate and chromatographic columns are easy to be blocked in a pyridine sodium hydroxide strong alkaline extraction system, the application constructs a stable detection scheme from sample pretreatment to instrument analysis by means of the following measures: nitrogen charging and oxygen removing of the extraction liquid, addition of a combination protective agent of sodium thiosulfate or sodium sulfite and disodium ethylenediaminetetraacetate, whole-process low-temperature light-avoiding operation, time restraint of sample injection within 1 hour after sample treatment, and protection of a chromatographic system by means of a continuous-use reverse-phase C18 chromatographic column under an alkaline condition with a pH value of 10 or more, a pre-column and a neutral gradient cleaning program. The peak area attenuation rate of heme iron within 1 hour is not more than 1.0% by the method, the column pressure of the chromatographic column can be controlled after batch sample injection, and the column pressure can be basically recovered after cleaning, so the method is suitable for quantitative determination of low-content heme iron in pilose antler and pilose antler powder, pilose antler extract, pilose antler blood products and other pilose antler related products.

The invention relates to the technical field of food processing, and discloses a blood clam instant three-belachan sauce and a preparation method of the blood clam instant three-belachan sauce. A blood clam body fluid concentrated solution; 3, Blachan sauce; fishing out the seasoning liquid; a blood clam shell extract; a color protection stabilizer; the blood clam instant three-belachan sauce comprises the following components in percentage by weight: 25-30% of blood clam meat granules, 18-20% of blood clam body fluid concentrated solution, 35-40% of three-belachan sauce, 8-10% of fishing seasoning liquid and 0.3-0.5% of blood clam shell extract. The blood clam body fluid concentrated solution contains a color protection stabilizer, and the color protection stabilizer comprises disodium ethylene diamine tetraacetate, a blood clam shell extract and heme iron collagen peptide; disodium ethylene diamine tetraacetate in the color protection stabilizer is beneficial to chelating Fe < 2 + >, so that an oxidation chain reaction is blocked; the blood clam shell extract is beneficial to forming a calcium film and blocking permeation and diffusion of oxygen; the heme iron collagen peptide can inhibit protein denaturation by wrapping heme, and a color protection system formed by compounding the three components effectively blocks a Fe < 2 + > oxidation path, so that the mechanism of active components is reserved.

To provide a novel method for producing heme iron-containing cytochrome c peroxidase.SOLUTION: A method for producing cytochrome c peroxidase includes: a PHB accumulation step of aerobically culturing a halophilic bacterium belonging to the genus Halomonas in the medium for 10 hours or more to cause the halophilic bacterium to accumulate poly-3-hydroxybutyrate; a 3HB secretion step of microaerobically culturing the halophilic bacterium in the medium while adjusting the pH to cause the poly-3-hydroxybutyrate accumulated within the halophilic bacterium to be secreted into the medium as 3-hydroxybutyrate; and a recovery step of recovering the cytochrome c peroxidase from the medium. The pH during microaerobic culture is adjusted to 9.0 or more and 12.0 or less.SELECTED DRAWING: None

The invention provides a heme iron cytochrome P450 enzyme mutant with improved enzyme electrocatalysis rate and application thereof. The heme iron cytochrome P450 enzyme mutant is obtained by performing site-directed mutagenesis on heme iron cytochrome enzyme with an amino acid sequence as shown in SEQ ID NO: 1 as a parent; according to the present invention, the 82nd leucine of SEQ ID NO: 1 is subjected to site-specific mutagenesis to obtain glutamine, the 167th phenylalanine of SEQ ID NO: 1 is subjected to site-specific mutagenesis to obtain methionine or the 280th valine of SEQ ID NO: 1 is subjected to site-specific mutagenesis to obtain threonine, such that the mutant with the improved enzyme electro-catalysis rate is obtained so as to solve the technical problem of low enzyme electro-catalysis rate of the heme iron cytochrome enzyme in the prior art;