The invention relates to the field of
aerospace biology and
cell biology, in particular to an in-vitro preparation method of myocardial cells under a
supergravity condition. Comprising the following steps: step 1, performing
adherent culture on myocardial cells on a two-dimensional matrix; 2, mixing the myocardial cells subjected to two-dimensional culture in the step 1 with
matrigel to form a three-dimensional culture
system; step 3, placing the three-dimensional culture
system obtained in the step 2 in a
supergravity environment for culture; wherein the gravity intensity of the
supergravity environment is 3G, and the
treatment time is 12-96 hours. The invention provides a complete and repeatable
myocardial cell in-vitro preparation method under the supergravity condition for the first time. A stable 3G supergravity environment can be provided, the damage of high
shear force to cells is effectively reduced, the core pain point that a traditional supergravity experiment depends on expensive and rare parabola flight or a large centrifugal
machine is solved, and the research cost and the technical threshold are greatly reduced.