76 results about "Saccharic acid" patented technology

The invention discloses a preparation method of magnesium threonate. The preparation method comprises the following steps: S1, preparing a stable glucose-magnesium six-membered ring compound; s2, concentrating the reaction liquid, crystallizing, and drying in vacuum to obtain a glucose-magnesium six-membered ring compound; s3, dissolving the compound in water, and adding Fe < 2 + > to adjust the pH value to 2.5-3.0; s4, the solution obtained in S3 is heated, H2O2 is dropwise added, Fe < 2 + > in the solution catalyzes H2O2 to generate hydroxyl radicals, the glucose-magnesium compound is selectively cracked, and magnesium threonate is generated; s5, adding Na2S2O3 to terminate the reaction, and filtering to remove Fe (OH) 3 precipitate; concentrating the filtrate under reduced pressure until the filtrate is sticky, standing at low temperature, and separating out a magnesium threonate crude product; according to the method, the glucose-magnesium six-membered ring compound is used as a raw material and is converted into magnesium threonate through Fenton reaction oxidative cracking, and the purified magnesium threonate is high in purity, low in Fe residue, mild in process condition and suitable for large-scale production.

The invention discloses a synthesis method of magnesium L-threonate. The synthesis method comprises the following steps: S1, preparing food-grade calcium L-threonate into an aqueous solution; s2, oxalic acid is added into the aqueous solution according to the molar ratio of 1: 1, and ultrasonic treatment is conducted for 30 min or above; s3, performing water bath treatment on the reaction mixed solution in a water bath at 0-4 DEG C, and then filtering to remove precipitates to obtain an L-threonic acid solution; s4, adding excessive magnesium oxide micro powder into the L-threonic acid solution, reacting, naturally cooling to room temperature, and filtering to remove precipitate to obtain an L-magnesium threonate solution; and S5, adding absolute ethyl alcohol into the magnesium L-threonate solution, then filtering to obtain a precipitate, washing the precipitate with absolute ethyl alcohol, and carrying out vacuum drying until the weight is constant, thereby obtaining the magnesium L-threonate. The invention further discloses the synthesis method and application in the pharmaceutical field. The synthesis efficiency is relatively high, the overall yield is also high, and it can be ensured that the purity of the synthesized magnesium L-threonate is high.

The invention provides a shikimic acid pathway artificial operon and a construction method and application thereof.According to the artificial operon, shikimic acid pathway related genes are integrated and connected in different modes, a trc promoter and an RBS ribose binding site sequence are optimized, and the artificial operon formed through combination has the advantages of being stable, efficient and wide in applicability; different from a relatively extensive shikimic acid way transformation mode in the past, the operon can obviously improve the accumulation capacity of aromatic amino acid, and when the operon is applied to an L-phenylalanine production strain, the yield of L-phenylalanine reaches 105 g / L, and the saccharic acid conversion rate reaches 26.5%.

The invention provides a preparation method and application of magnesium L-threonate, and belongs to the technical field of medicines. The preparation method comprises the following steps: dissolving L-ascorbic acid in water, adding magnesium hydroxide and alkaline magnesium carbonate, stirring, dropwise adding hydrogen peroxide, heating, stirring and reacting, filtering a product, purifying and concentrating by temperature-sensitive molecularly imprinted hydrogel while the product is hot, adding an ethanol water solution into a concentrated solution, standing, crystallizing, filtering, washing and drying to obtain the magnesium L-threonate. And cooling the temperature-sensitive molecularly imprinted hydrogel after adsorption to room temperature, washing and repeatedly using the temperature-sensitive molecularly imprinted hydrogel. The magnesium L-threonate is simple in preparation method, high in purity and high in yield, meanwhile, the slow-release magnesium L-threonate is prepared, and the magnesium L-threonate is low in toxicity, good in biocompatibility, large in drug loading capacity, low in economic cost and good in drug effect and has wide application prospects in preparation of anti-depression drugs.

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention provides a liquid fertilizer for improving tomato fruit saccharic acid quality and application, and belongs to the technical field of fertilizers. The liquid fertilizer for improving the tomato fruit saccharic acid quality provided by the invention is prepared from the following components in percentage by weight: 42 to 47 percent of organic nutrient solution, 10 to 15 percent of water-soluble macroelement compound, 1.5 to 2.5 percent of natural organic acid, 3.5 to 4.5 percent of plant source amino acid compound, 3 to 5 percent of compound biostimulant, 0.02 to 0.07 percent of polyglutamic acid and 0.2 to 0.4 percent of sugar alcohol medium trace element compound liquid, and 28-33% of sterile water. Through optimized formula design, the organic liquid fertilizer is rich in plant nutrition and bioactive substances, can effectively improve the healthy growth state of tomatoes, and meanwhile, remarkably improves the fruit quality.

A detergent building composition, comprising (i) one or more sugar oxidation products; and (ii) at least one performance augmenting additive selected from the group consisting of aluminates, silicic acid, sodium silicate, potassium silicate, monosilicates, carbonates, silanes, siloxanes, amino acids, urea, boric acid, borate derivatives, molybdates, titanates, chelating agents, sugar alcohols, carboxylates, acids, and combination thereof. A detergent building composition, comprising (i) glucaric acid, gluconic acid, salts thereof, lactones thereof or combinations thereof; and (ii) at least one performance augmenting additive selected from the group consisting of aluminates, silicic acid, sodium silicate, potassium silicate, monosilicates, carbonates, silanes, siloxanes, amino acids, urea, boric acid, borate derivatives, molybdates, titanates, chelating agents, sugar alcohols, carboxylates, acids, and combination thereof.

The invention discloses a method for producing L-arginine through escherichia coli fermentation. The escherichia coli has the characteristics of high L-arginine yield and low heteroacid content. In the fermentation process, fed-batch sugar and sodium glutamate are adopted according to the thallus concentration OD600 and the fermentation period; the concentration of glutamic acid is controlled by feeding sodium glutamate or glutamic acid precursor sodium succinate and sodium citrate, the arginine acid production and sugar acid conversion rate is increased, the content of heteroacid is reduced, the final acid production can reach 13.9% at most, the sugar acid ratio is 57.2%, and the heteroacid content is not higher than 0.3%. The process solves the control difficulty in the industrial process of arginine, and has great commercial value.

The invention relates to a polyacid composite oil-controlling and acne-removing gel and a preparation method thereof, and belongs to the technical field of skin care products. The polyacid composite oil-controlling and acne-removing gel is prepared from the following components in percentage by mass: 2% of salicylic acid, 3%-4% of glycolic acid, 0.5%-1.5% of mandelic acid, 0.5%-1.0% of lactobionic acid, 0.5%-1.0% of gluconolactone, 1.2%-1.6% of microcrystalline cellulose (and) sphingomonas fermentation product extract (and) cellulose gum, 2%-5% of butanediol, 0.3%-0.5% of asiaticoside-II, 0.5%-1.5% of centella asiatica, 0.5%-1.5% of vitamin E and the balance of water. And the balance of deionized water. By adding micro-crystalline cellulose (and) sphingomonas fermentation extract (and) cellulose gum, system stability under compounding of five organic acids is realized, semitransparent gelatinous texture is obtained, the composition is suitable for point coating use mode, impression is not affected, oil-controlling and acne-removing effects are improved under the condition of low organic acid content, and the composition belongs to skin-care polyacid products and has a broad market prospect. The mild oil control and acne removal effects are realized, and the cream is suitable for oil-sensitive skin crowds.

The invention discloses development and application of a biosensor for detecting L-threonine, and belongs to the field of genetic engineering. The invention provides a construction method of a novel threonine biosensor and an application method for screening L-threonine high-yield mutants in an auxiliary manner through the biosensor. The biosensor can sensitively detect the concentration change of L-threonine. By combining with a high-throughput screening system, L-threonine high-yield strain mutants with improved production performance can be rapidly screened. By means of the biosensor, an L-threonine high-yield mutant LMTM35 is successfully screened from an L-threonine production strain LMT4 mutant library, 166 g / L of L-threonine is accumulated in a 5L fermentation tank after 45 hours, and the saccharic acid conversion rate is 0.67 g / g.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The invention discloses a genetically engineered bacterium for high yield of L-alanine as well as a construction method and application of the genetically engineered bacterium, a dominant strain is obtained by knocking out an alanine racemase gene dadX and integrating coding sequences of alanine dehydrogenase from different sources, and the shake flask yield is 10.8 g / L; the activity of a corresponding mutant M18L / E75A / A78S / I266V is 4.3 times that of a wild type; 6 by-product related genes are knocked out, meanwhile, a multi-copy strain integrated with alanine dehydrogenase is constructed, and the yield of E.coli A-LB6 in a 5L fermentation tank is increased to 133.3 g / L. Through a dynamic regulation and control strategy, an oxygen-sensitive promoter Pomp * is adopted to replace a phosphoenolpyruvate carboxylase promoter, so that succinic acid byproducts are reduced by 85.8%, and the yield reaches 143.5 g / L after 48 hours of aerobic and anaerobic two-stage fermentation. And finally, by regulating and controlling the ATP synthetase gene cluster atp promoter, the yield of L-alanine in a 50 L fermentation tank reaches 151.3 g / L, and the saccharic acid conversion rate reaches 95.2%. The combination of polygene knockout and dynamic metabolism regulation effectively enhances the biosynthesis ability of alanine, and provides an efficient engineering strain for industrial production.

The invention belongs to the technical field of daily-use chemistry, and particularly relates to a keratin regulating composition containing mandelic acid and having skin smoothing and brightening effects and application of the keratin regulating composition. The composition provided by the invention comprises mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate. According to the application, mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate are compounded, so that the activity of KLK family proteases, especially kallikrein 5 (KLK5), can be obviously promoted, the decomposition of desmosome between keratinocytes is promoted, epidermal cells lose cohesion and finally naturally fall off, and the effect of promoting the activity of KLK family proteases is achieved. The cutin exfoliation promoting effect is achieved, so that the problems of skin darkness, roughness, pore blockage, acne and the like are solved, the glossiness and fineness of the skin are improved, and healthy and bright skin is achieved.

A hydrogel for treating body cavity injury is derived from a chitosan derivative obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan by dehydration condensation. The hydrogel for treating the body cavity injury is granular, can be injected into a body cavity by utilizing an injector, and is used for treating the wound of the body cavity. A body cavity injury treatment kit is provided with a syringe, a tube connected to the syringe, and a granular body cavity injury treatment hydrogel filled into the syringe, the body cavity injury treatment hydrogel being derived from a chitosan derivative, the chitosan derivative is obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan through dehydration condensation.

The invention discloses recombinant escherichia coli for producing butyric acid and a construction method of the recombinant escherichia coli. The invention belongs to the technical field of biology, and particularly relates to recombinant escherichia coli for producing butyric acid and a construction method of the recombinant escherichia coli. The recombinant enterobacter disclosed by the invention is a recombinant bacterium obtained by enabling receptor enterobacter to enhance expression of a coding gene of pantothenic acid transport protein, the receptor bacterium is escherichia coli NZ-B016, and the obtained recombinant bacterium JH014 is recombinant escherichia coli obtained by integrating a promoter RBSL14 into an initiation codon ATG of a panF gene of the recombinant bacterium NZ-B016. After the recombinant strain JH014 is fermented for 96 hours, the strain OD550nm reaches 2.41, the yield of butyric acid reaches 4.22 g / L, the saccharic acid conversion rate reaches 0.81 mol / mol, and the recombinant strain JH014 has a wide application prospect.

The invention relates to the technical field of bioengineering, in particular to a protein mutant containing a DUF2283 structural domain and application of the protein mutant. Compared with a wild type DUF2283 structural domain-containing protein of corynebacterium glutamicum, the protein mutant containing the DUF2283 structural domain, provided by the invention, contains mutation of which the 6th aspartic acid is mutated into amino acids except the aspartic acid. The mutant can significantly improve the lysine synthesis efficiency of the strain, and further significantly improve the lysine yield and / or saccharic acid conversion rate of the strain. The mutant can be used for construction and screening of lysine or lysine derivative production strains, is beneficial to improvement of lysine yield and saccharic acid conversion rate of the lysine production strains, and has important significance in improvement of fermentation production performance of the strains and reduction of production cost.

The application uses cheap and easily available hexose acid as a starting material, first obtains two precursors of 2,5-furan dicarboxylic acid synthesis, namely hexahydroxy acid and 4-deoxy-5-dehydro-hexahydroxy acid through enzyme catalytic reaction, and then 4-deoxy-5-dehydro-hexahydroxy acid is dehydrated under acidic conditions to obtain 2,5-furan dicarboxylic acid.

The invention discloses a method for preparing calcium threonate by using calcium galactobionate as a raw material and application of the calcium threonate. The method comprises the following steps: S1, adding the calcium galactobionate into hydrochloric acid to generate galactobionic acid and calcium chloride; s2, under the protection of nitrogen, soluble ferrite is added into the mixed solution obtained in the S2, H2O2 is dropwise added, Fe < 2 + > catalyzes H2O2 based on the Fenton reaction principle to generate hydroxyl free radicals, galactobionic acid C4-C5 sites are directionally attacked to break, and erythritol is generated; s3, adjusting the pH value of the reaction system, and separating Fe (OH) 3 colloidal particles; s4, adjusting the pH value of the erythritol dispersion liquid to 9-10, and performing alkali-induced ring opening and epimerization on the erythritol to obtain calcium ions in the threonic acid complexing solution; s5, crystallizing and purifying the calcium threonate obtained in S4 to obtain a target product. The calcium threonate obtained by the invention is applied to a calcium supplement; the method is high in catalytic selectivity, low in byproduct content and convenient to separate and purify.

To provide an acidic oil-in-water emulsified seasoning that exhibits superior melt-in-the-mouth and form retention after baking, and has superior richness of taste after baking.SOLUTION: The present invention provides an acidic oil-in-water emulsified seasoning, comprising enzyme-treated egg yolk, a saccharide of disaccharide or lower, an acidulant, a thickener, and edible oil and fat, wherein the content of the saccharide of disaccharide or lower is 10 mass% or more relative to the total amount of the acidic oil-in-water emulsified seasoning, the content of the saccharide of disaccharide or lower is 14.0 pts.mass or more relative to 1 pt.mass of the acidulant, the thickener includes a cold-water swelling tamarind seed gum, the content of the cold-water swelling tamarind seed gum is 0.05 mass% or more and 1.2 mass% or less relative to the total amount of the acidic oil-in-water emulsified seasoning, and the content of the edible oil and fat is more than 40 mass% relative to the total amount of the acidic oil-in-water emulsified seasoning.SELECTED DRAWING: None

The invention discloses a preparation method of ferrous L-threonate, and relates to the technical field of organic chemical synthesizing.The preparation method comprises the steps that vitamin C serves as a raw material and is oxidized through hydrogen peroxide to obtain threonic acid, residual hydrogen peroxide is removed through high temperature, and the threonic acid reacts with ferrous carbonate under the nitrogen protection condition to generate the ferrous L-threonate; and filtering and concentrating under reduced pressure to obtain a ferrous L-threonate crude product, and recrystallizing and purifying the crude product to obtain a ferrous L-threonate pure product. The process has the advantages of good process stability, high purity of pure products, cheap and easily available raw materials, simplicity in operation, high safety, environmental friendliness and the like.

The invention discloses a method for producing lactic acid by efficiently utilizing lignocellulose pretreatment liquid through fermentation. The method comprises the steps of preparing lignocellulose detoxification saccharification liquid and producing lactic acid through fermentation. The preparation process of the lignocellulose detoxified saccharified liquid mainly comprises the following steps: adding hydrogen peroxide under an acidic condition to react for a period of time, then adding manganese dioxide to remove excessive hydrogen peroxide, and then adding cellulase to obtain the lignocellulose saccharified liquid; the process for producing lactic acid by fermentation comprises the following steps: activating seeds, preparing a seed solution, introducing gas for a period of time after inoculation, and then keeping anaerobic fermentation to produce lactic acid. According to the method, most of aldehydes such as furfural, 5-hydroxymethylfurfural and vanillin and phenolic inhibitors such as acid-soluble lignin in the pretreatment liquid are removed. Divalent manganese ions generated in the detoxification step and a proper amount of ventilation operation in the initial stage of fermentation are beneficial to growth metabolism and fermentation production of lactic acid bacteria, and the sugar-acid conversion rate reaches up to 96% or above.

A beta-glucan compound capsule comprising: a capsule body, wherein the capsule body comprises a first capsule shell and a second capsule shell, the first capsule shell having a first opening at one end and a rounded first closure at the other end, with a first snap-in structure on the outer edge; the second capsule shell is transparent, with a second opening at one end and a circular second closed end at the other end, with a second snap-in structure on the inner edge, wherein the first opening of the first capsule shell and the second opening of the second capsule shell interlock, and wherein the first snap-in structure and the second snap-in structure interlock to form a closed receiving space;and a content housed in the receiving chamber, the composition of which comprises β-polydextrose, glutamic acid, Lactobacillus paracasei, zinc glycosaminoglycate, selenium yeast, magnesium stearate and silicon dioxide.

The application discloses genetically engineered bacteria for synthesizing L-tryptophan and application thereof. The application realizes the following through two key gene regulation strategies (serA down-regulation and serB up-regulation): the accumulation amount of glutamic acid by-products is significantly reduced from 8-20 g / L to below 1.14 g / L, and the reduction ranges are 87.74% and 90.3% respectively; the sugar acid conversion rate (glucose / L-tryptophan) of L-tryptophan is increased from 19.2% to 20.9% and 21.3% respectively; without additional addition of expensive cofactors or inducers, the cost-effectiveness is significant.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol by catalyzing hemicellulose hydrolysate through a biological enzyme, which comprises two stages: in the first stage, adding hemicellulose hydrolysate, xylose reductase, arabinose dehydrogenase and glucose dehydrogenase to form a fermentation system, adjusting the pH value of the system to 8.0-9.5, and fermenting, adding L-arabinose until no L-arabinose residue exists in the fermentation liquid to obtain a fermentation reaction liquid I; in the second stage, the pH value of the fermentation reaction liquid I is adjusted to 6.5-7.5, fermentation continues to be conducted, and a fermented product containing xylitol is obtained. According to the method for preparing the xylitol through the biological enzyme catalysis method, cheap hemicellulose hydrolysate can be directly adopted as a raw material, and high-purity xylose does not need to be used as the raw material; the xylose reductase, the arabinose dehydrogenase and the glucose dehydrogenase used in the method are wide in gene source, the three-enzyme one-pot catalytic process is simple and efficient, the conversion rate of xylose and the yield of xylitol both can exceed 99%, the content of impurity arabitol in the product is low, other impurity saccharic acid is easy to separate, and the method is suitable for industrial production. The problems of complex process, high separation difficulty and high cost in the xylitol refining process are successfully solved.

A method of preparing polylactic acid (PLA) microsphere and polylactic-co-glycolic acid (PLGA) microsphere is provided, including the following steps. A first solution is provided, including polylactic acid or polylactic-co-glycolic acid and an organic solvent. A second solution is provided, including polyvinyl alcohol, sodium carboxymethyl cellulose and an aqueous solution. The first solution is added to the second solution and, at the same time, the second solution is agitated until polylactic acid is solidified to form a plurality of polylactic acid microspheres, or until polylactic-co-glycolic acid is solidified to form a plurality of polylactic-co-glycolic acid microspheres. The polylactic acid microspheres or polylactic-co-glycolic acid microspheres are collected. The polylactic acid microsphere and the polylactic-co-glycolic acid microsphere are respectively the accumulation of the polylactic acid and polylactic-co-glycolic acid, having a smooth surface and a mean number particle size distribution (MN) from 10 μm to 80 μm.

The invention relates to the field of microorganisms, and discloses corynebacterium glutamicum and application thereof. Corynebacterium glutamicum with the preservation number of CGMCC No.35081 can tolerate the environment with the pH value of 5 and still has glutamic acid fermentation performance, the strain can still have high glutamic acid yield and sugar-acid conversion rate under the acidic condition with the pH value of 5.7-5.9, the performance of the strain is not reduced compared with that under the neutral fermentation condition, and even can be maintained at a considerable level, and the strain can be applied to the field of fermentation. By means of the characteristic, the adding amount of liquid ammonia in the later period of fermentation can be greatly reduced, and therefore raw material consumption and comprehensive production cost in the production process are remarkably reduced.

The invention discloses a double-target nerve conduction supporting nutritional composition and a preparation method thereof, and belongs to the technical field of nutritional products. The composition is prepared by compounding DHA algal oil, phosphatidylserine, EGCG and B vitamins with xanthoceras sorbifolia bunge oil nervonic acid and magnesium L-threonate which serve as dual-core targets in parts by mass, a'myelin sheath repair + signal transduction acceleration 'dual-target synergistic system is formed, membrane structure maintenance, oxidation resistance, inflammation resistance and neurotransmitter synthesis support are achieved at the same time, and the composition has the advantages of being simple in preparation process, low in cost and easy to popularize and apply. Neural conduction efficiency is systematically improved and instruction-execution latency is shortened. The problems of non-uniform mixing and caking of liquid oil and powder are solved through microencapsulation, the process is mature, the safety is high, and the stability is good. Animal experiments show that the composition can shorten the time of simple reaction by 30.5% (plt, 0.01) and shorten the time of selective reaction by 29.9% (plt, 0.01), has a non-obvious and unexpected technical effect, and is suitable for long-term nutritional supplement of middle-aged and elderly people and stroke convalescent people.

The invention discloses a metabolic antagonist solution which is obtained by dissolving a metabolic antagonist in water, the metabolic antagonist is L-2-enol hexolactone and / or 2-deoxy-D-glucose, and the invention also discloses an application of the metabolic antagonist solution in storage of crude rosin. The metabolism antagonist solution provided by the invention aims at glycolysis and energy metabolism of impurities such as pine needles and barks, and discoloration and degradation of the crude rosin in the storage process are blocked from the source; the L-2-enol hexanoic acid lactone oxidation product enters cells through glucose transporters such as GLUT1 / 3 / 4, competes for binding sites with glucose, and interferes with glycolysis and energy metabolism of impurities such as pine needles and barks; the 2-deoxy-D-glucose has a pyranoid ring conformation highly similar to that of glucose, is acidified into 2-deoxyglucose-6-phosphoric acid by hexokinase, and hijacks a glycolytic pathway, resulting in metabolic blocking and energy depletion.

The application discloses a preparation method and application of chitooligosaccharide alduronic acid and belongs to the technical field of marine bioengineering. The preparation method comprises the following steps: after beta-chitin and a cleaving polysaccharide monooxygenase SmLPMO10 are dissolved in a Tris-hydrochloric acid buffer solution, a reducing agent is added, and an enzymatic reaction is carried out; after the reaction is carried out for 24 hours, the reducing agent is supplemented, the reaction is continuously carried out, the reducing agent is supplemented once every 24 hours, after the reaction is carried out for 96 hours, supernatant is obtained by filtration, and the chitooligosaccharide alduronic acid is obtained after inactivation, centrifugal removal of supernatant, desalination and freeze-drying. The chitin can be cleaved by using a single cleaving polysaccharide monooxygenase, without additional addition of chitinase, and the reaction condition is mild, so that the generation of by-products and environmental pollution are avoided. The application provides an environment-friendly preparation method of chitooligosaccharide alduronic acid, and the research and development of a green conversion technology of chitin and chitin-like biomass resources are greatly promoted.

To provide processes for preparing glucaric acid and / or lactones thereof.SOLUTION: Various processes for preparing aldaric acids, aldonic acids, uronic acids, and / or lactones thereof are described. For example, processes for preparing a C2-C7 aldaric acid and / or lactones thereof by the catalytic oxidation of a C2-C7 aldonic acid and / or lactones thereof and / or a C2-C7 aldose are described.SELECTED DRAWING: None