204 results about "Saccharic acid" patented technology

The invention discloses a method for preparing activated carbon for a biomass-based super capacitor, in particular relates to a new method for preparing activated carbon with high specific surface area and high specific capacitance by the steps of hydrolysis of biomass with concentrated acid, in-situ polycondensation and carbonization of saccharic acid solution and activation under certain conditions. The method comprises the following specific steps of: firstly, hydrolyzing biomasses with inorganic acid with a certain concentration, and filtering to obtain a saccharic acid solution and filter residues; adjusting the concentration of the saccharic acid solution, performing polycondensation and carbonization to prepare hydrothermal carbon; mixing the hydrothermal carbon with an activating agent in a certain mass ratio for activating to finally obtain the activated carbon for the super capacitor. The method for preparing activated carbon for the super capacitor by using biomasses is a pollution-free process route meeting the requirement of sustainable development.

A compound of ferrous L-threonate with structure (I), its compostions and methods useful for iron supplementation for mammals, particularly for human body to improve and treat nutritional iron-deficiency anemia, blood loss anemia and hemolytic anemia.

The invention discloses a preparation method of biomass-based colloidal carbon and in particular relates to a colloidal carbon ball using xylose or glucose as a precursor. The colloidal carbon ball is prepared by hydrolyzing biomass by using dilute acid or concentrated acid respectively to obtain saccharic acid solution of xylose and glucose and performing in-situ polycondensation and carbonization on the saccharic acid solution. The preparation method particular comprises the following steps of: hydrolyzing the biomass by using dilute acid at a certain concentration; filtering to obtain xylosic acid solution and filter residues; adjusting the concentration of the saccharic acid solution and performing polycondensation and carbonization to prepare the colloidal carbon ball with grain size of 160 to 1,800 nm; performing alkali boiling on the residues to remove lignin; performing hydrolysis reaction on the residues and the concentrated sulfuric acid; filtering to obtain acid solution of the glucose; and performing in-situ polycondensation and carbonization to prepare the colloidal carbon ball with grain size of 180 to 2,000 nm. The method for preparing the colloidal carbon from the biomass is a green production route of sustainable development.

The invention provides a genetically engineered bacterium for high-yielding L-valine. A construction method of the genetically engineered bacterium comprises the steps that starting from an escherichia coli W3110, an acetolactate synthase gene alsS of a bacillus subtilis is integrated on a genome of the escherichia coli W3110 and subjected to high expression; an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolytic enzyme mutant R290E/K292D gene spoT is integrated on the genome of the escherichia coli W3110 and subjected to high expression; genes of frdA, frdB, frdC and frdD of four subunits of a lactic dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB and fumaric reductase on the genome of the escherichia coli W3110 are knocked out; a branched chain amino acid transaminasegene ilvE of the escherichia coli is replaced with leucine dehydrogenase gene bcd of the bacillus subtilis; and an acetyl-hydroxyl acid isomerized reductase gene ilvC of the escherichia coli is replaced with an encoding gene of a mutant L67E/R68F/K75E. According to the genetically engineered bacterium for the high-yielding L-valine, an L-valine fermentation method is further modified. Double-phasedissolved oxygen control is adopted, and the L-valine yield and the saccharic acid conversion rate are improved.

The invention discloses jujube-acetic acid beverage and a processing technic thereof. The processing technic comprises the following steps: pretreatment of raw materials, pulp preparation, ultra-high-pressure enzymolysis, saccharic acid adjustment, vaccination, alcohol fermentation, acetic acid fermentation, clarification, sterilization, mixng and bottling. Based on the traditional process, the invention adopts a new technique and the ultra-high-pressure assisted enzymolysis, thereby not only improving the yield of jujube juice, but also reducing the working time; and due to the liquid-state shaker fermentation, the method shortens the fermentation time, facilitates the reduction of the production cost of the enterprise, and improves the economic efficiency. In addition, with the optimized mixing scheme, the invention solves the problem of single taste. The jujube-acetic acid beverage produced in the invention has the advantages of good taste, moderate and refreshing sweetness and sourness, high clarity and transparency, fruity palate and delicate flavor.

The invention discloses a method for co-producing a plurality of saccharic acids from cellulose fuel ethanol. The method comprises the following steps: performing dilute acid hydrolysis pretreatment or dilute acid steam explosion pretreatment on plant fiber raw materials to obtain a hemicellulose pre-hydrolysate and cellulose-enriched solid material through solid-liquid separation, wherein an enzyme hydrolysate is obtained by neutralizing and performing cellulose enzyme hydrolysis on the solid material, and fuel ethanol is produced by anaerobically fermenting the enzyme hydrolysate through saccharomyces cerevisiae or zymomonas mobilis; and a plurality of saccharic acids are produced by whole-cell co-catalyzing through gluconobacter oxydans or pseudomonas fragi under an aerobiotic condition after the hemicellulose pre-hydrolysate is concentrated and neutralized. The plurality of saccharic acids can be co-produced from fuel ethanol prepared from the plant fiber raw materials, the inhibiting effect of hemicellulose pre-hydrolysate sugar fermentation can be effectively overcome, the utilization efficiency of various carbohydrate substances in the plant fiber raw material can be obviously improved, the pollution load in industrial wastewater is effectively reduced, thereby improving the economic benefit of bio-refinery of the plant fiber raw materials. The utilization rate of total carbohydrate in the raw materials is over 80%, and the carbohydrate conversion rate is more than 90%.

Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

The invention pertains to the technical field of lactic acid preparation, and relates to a bacillus coagulans used to preparing L-lactic acid and application methods thereof. Pentose or hexose is taken as raw material, or agricultural and industrial wastes containing the pentose, the hexose or combinations thereof are taken as raw materials. Ferment is performed on the raw materials to obtain theL-lactic acid. By applying the method of the invention, the highest yield can reach 195g/L, optical purity is greater than 99%, the highest conversion rate of saccharic acid can reach 0.98, and throughput of the ferment process is 2.7g/L/h. By using the bacillus coagulans XZL4(DSM No.23183) and the bacillus coagulans XZL9(DSM No.23184) provided in the invention, high concentration of L-lactic acid can be produced by directly fermenting various reducing sugar in by-products produced in xylitol productions. According to the invention, low cost is achieved, and production efficiency is improved at the same time. The bacillus coagulans and the methods are applicable to popularization and application in industrial productions.

The invention discloses a preparation method of azithromycin intermediate, in particular to a preparation method of azithromycin intermediate 9- deoxidation-alpha- heterocyclic nitrogen-9a-erythromycin A, belonging to the field of pharmaceutical chemical synthesis. The preparation method comprises the steps of: in acid aqueous solution, under the action of potassium borohydride or sodium borohydride, carrying out reduction reaction on erythromycin A 6, 9-imine ether, adding organic saccharic acid for hydrolysis reaction, and obtaining the 9-deoxidation-alpha-heterocyclic nitrogen-9a-erythromycin A. By adopting the method, the hydrolysis of boric acid ester is thorough, the products of acid degradation are less, the yield is high, the production cost of azithromycin is remarkably reduced, and the industrialized production can be very smoothly carried out.

The invention discloses a fermentation production method for citric acid, and in particular relates to a continuous fermentation production method for citric acid. A citric acid continuous fermentation system consists of a culture medium storage tank, a seed culture tank, two or more serially connected fermentation tanks and a fermentation mash storage tank. By the citric acid continuous fermentation system, a fermentation period can be saved, the conversion rate of saccharic acid and the utilization rate of the fermentation tanks are improved, and stages such as growth and development and saccharification effects of aspergillus niger on citric acid production bacteria, fermentation and acid production and the like are controllable sectionally.

The invention discloses a method for producing polymer grade L-lactic acid. The method comprises the following steps: inoculating bacillus sp. into a fermentation culture medium taking maize straw hydrolysate as an only carbon source for fermentation culturing to obtain the L-lactic acid; the mass ratio of glucose, xylose and arabinose in the maize straw hydrolysate is (27-29): (8-10):1; and the bacillus sp. is bacillus coagulans XZL4DSM No.23183. With adoption of the method, the glucose in the maize straw hydrolysate can be efficiently utilized; the xylose also can be utilized; the yield of the L-lactic acid can reach 80g/liter; the conversion rate of saccharic acid can reach 94-98%; the condition that D-lactic acid exists in a fermentation liquid cannot be detected; and the optical purity can reach 100%; and in addition, the method also can lower the cost of the fermenting the raw materials and is beneficial for industrial production.

The invention discloses a method for preparing glycosyl amide modified polysiloxane. The method comprises the following steps of: reacting cyclosiloxane, hexamethyldisiloxane and aminosilane serving as raw materials under the action of a catalyst at the temperature of between 30 and 220 DEG C for 4 to 8 hours, wherein the molar ratio of the cyclosiloxane to the aminosilane to the hexamethyldisiloxane is 0.25-2.25:3-5:1; the molar ratio of the catalyst to the sum of the cyclosiloxane, the aminosilane and the hexamethyldisiloxane is 0.1 to 1; inactivating the catalyst after the reaction is finished; performing reduced pressure distillation to obtain the amino-polysiloxane; reacting the amino-polysiloxane and glycosyl lactone or saccharic acid in low-carbon alcohol serving as a solvent at the temperature of between 30 and 200 DEG C for 5 to 10 hours, wherein the molar ratio of primary amine of the amino-polysiloxane to the glycosyl lactone or saccharic acid is 1-5:1; and evaporating off the solvent to obtain the glycosyl amide modified polysiloxane after the reaction is finished. The method has the advantages of low cost, no toxicity and large-scale industrialization.

The invention relates to the field of fermentation, in particular to an L-lysine fermenting method. The L-lysine fermenting method comprises the following steps: inoculating a lysine first stage seed medium with a lysine shake flask seed, culturing the seed till the seed is mature, then, inoculating the seed in a lysine second stage seed medium for culturing till the seed reaches a mature standard of a lysine second stage seed, inoculating a lysine fermenting medium with the second stage seed, and under conditions of a fed-batch carbon source and a fed-batch nitrogen source, performing fermentation culture till the end of fermentation, wherein the nitrogen source in the lysine primary seed medium, the lysine secondary seed medium and the lysine fermenting medium is ammonium chloride, and during fermentation culture, the fed-batch nitrogen source is an ammonium chloride solution. By the L-lysine fermenting method, the final content of lysine, the total acid amount and the conversion ratio of saccharic acid can be significantly increased.

The invention discloses a novel black and red Chinese wolfberry fruit compound fermented beverage and a preparation method thereof and belongs to the processing field of microbial fermentation technologies of foods. The preparation method mainly comprises the steps of pretreatment of raw materials, juicing, ultrasonic assisted chitosan immobilized complex enzyme treatment, filtration, pasteurization, primary fermentation of a lactic acid bacterium composite leavening agent, secondary fermentation with active dry yeast and acetic acid bacteria, saccharic acid blending, centrifugation, pasteurization, filling and finished beverage. The preparation method is simple in process and low in processing cost, the prepared beverage has the flavor of Chinese wolfberry fruits and also has the smell produced through Chinese wolfberry fruit, a pleasant mellow aroma and soft sour, so that the beverage has more delicate taste, has no bad odor, homogeneous color and luster and good nutritional health-care functions; the total acid of the beverage is 2.83%, pH is 3.70, the aqueous protein of plants is 2.83g/L, the total phenol is 38.17mg/L, the carotenoid is 35.67ug/L, the alcoholic strength is 1% or below, and bacteria and escherichia coli are not detected out.

The invention relates to the field of fermentation, and particularly provides a method for fermenting L-lysine. Acetate is added into lysine primary seed culture media, lysine secondary seed culture media and lysine fermentation culture media, and formulas and fermentation processes for the various culture media are optimized. The method has the advantages that growth of lysine producing thalli and synthesis of lysine can be promoted, accordingly, the endpoint lysine content, the total acid content and the saccharic acid conversion rate can be obviously increased, and the fermentation periods can be obviously shortened.

The invention discloses a method for increasing the yield of L-arginine synthesized from corynebacterium crenatum, and belongs to the technical field of bioengineering. By means of the method, recombinant corynebacterium crenatum of which a nitrogen transcriptional regulation factor AmtR is knocked out and ammonium transport protein is over-expressed is successfully constructed. By adopting a strategy of batch fermentation by a 5-L fermentation tank and optimizing fermentation conditions, finally the recombinant corynebacterium crenatum Cc5-5/pXMJ19-amtB is fermented for 96 h, the L-arginine yield of the recombinant corynebacterium crenatum reaches 60.9+/-1.31 g/L, the yield is increased by 30.56% compared with an original strain of corynebacterium crenatum original strain SYPA5-5, the production intensity reaches 0.634 g/L.h, and the saccharic acid conversion rate is 0.36+/-0.018 g/g, so that high yield of the L-arginine is realized. Meanwhile, utilization of NH4+ is increased in therecombinant corynebacterium crenatum, so that it is shown that knockout of the nitrogen transcriptional regulation factor and overexpression of the ammonium transport protein have a remarkable effecton the yield of the L-arginine.

The invention discloses escherichia coli capable of producing L-alanine through fermentation and application of the escherichia coli and belongs to the technical field of biological engineering. According to the escherichia coli, aspartase AspA and L-aspartic acid-beta-decarboxylase AsD are introduced into the escherichia coli capable of producing fumaric acid through the fermentation; furthermore, a YgaW gene is over-expressed to strengthen the transport ability of the L-alanine in recombinant bacteria, so that a novel strain capable of fermenting glucose to produce the L-alanine is constructed; when the recombinant strain is fermented in a fermentation tank for 45h, the yield of the L-alanine reaches 147g/L and the saccharic acid conversion rate is 79.0 percent.

The invention discloses actinobacillus succionogenes capable of producing succinic acid with high yield and a method for screening and producing the succinic acid by a fermentation method. The actinobacillus succinogenes takes CGMCC1593 as the original strain and is obtained by blending a plurality of turns of protoplast in a progressive way through a method of '96-pore plate culture, HPLC concentration detection and then anaerobic bottle re-screening'. The actinobacillus succinogenes has already been preserved on February 26, 2012 in China Center for Type Culture Collection with the preservation number CCTCCNO: M2012036. The actinobacillus succinogenes adopts fed-batch culture in a 5 to 15 L fermentation tank, 95.6 g/L succinic acid is produced in 48 hours, the production intensity is 1.99 g/(L.h), and the saccharic acid transformation rate is 0.71 g/g. Compared with other bacterial strains at home and abroad, the actinobacillus succinogenes has the advantages of high yield and lowerproduction cost.

The invention discloses a corynebacterium-glutamicum recombinant strain for increasing the conversion rate of L-phenylalanine saccharic acid and belongs to the field of metabolic engineering. The recombinant strain is characterized in that genes such as aroP, aceE, ldh and pstI are deficient, iolT2-ppgk gene is integrated on the original pstI site, and simultaneously regulatory expression plasmid containing eight key enzymes in the L-Phe synthesis path is transferred. The corynebacterium-glutamicum recombinant strain disclosed by the invention has the advantages that the accumulated amount of PEP (phosphoenolpyruvate) of an L-Phe intermediate product, the yield of the L-Phe and the conversion rate of the L-Phe for saccharic acid of glucose are obviously increased, the flowing direction of a carbon source is changed to reduce or block accumulation of side products, and the means of the metabolic engineering can be applied in synthesis of other metabolites adopting PEP as a precursor.

The invention discloses a method of co-catalytically synthesizing various saccharic acids by virtue of the synergism of metal ions and a selective regulation whole-cell, and relates to the technical field of synthesizing the saccharic acids by biologically catalyzing sugar. The method is mainly characterized in that in a mixed sugar solution or lignocelluloses hydrolysate containing glucose and xylose, 1g/L to 10g/L of gluconobacter oxydans is used as a biological catalyst to co-catalyze the glucose and the xylose under an oxygen-supply condition so as to synthesize the saccharic acids. Metal salt of a given concentration containing zinc ions and trivalent iron ions is added so as to selectively inhibit the catabolism of a cell on the gluconic acid, but a dehydrogenation catalytic reaction of the xylose and the glucose is hardly affected, thus an effect of the cell for co-catalyzing and high-efficiently synthesizing a gluconic acid (salt) and xylonic acid (salt) product can be further achieved, and the reaction time is effectively shortened. By adopting the method, the utilization rate of the glucose and the xylose reaches 100 percent, and the yield of the gluconic acid is more than 70 percent, and the yield of the xylonic acid is more than 92 percent, and the total concentration (mass concentration) of the product gluconic acid (salt) and xylonic acid (salt) can be more than 30 percent.

The invention belongs to the technical field of feed processing, particularly relates to a method for preparing a piglet syrup feed without fish meal and plasma proteins. After insoluble constituents are subjected to coarse grinding, water, chicken egg pulp or duck egg pulp, lactic acid and other constituents are added to be stirred, mixed and evenly prepared, and wet process superfine grinding and pulp grinding are performed through a ball grinding mill or a colloid mill to prepare starch proteolipid pulp. Soluble constituents are added into the water to prepare saccharic acid calcium micro-water pulp. The saccharic acid calcium micro-water pulp and starch proteolipid pulp are mixed, coarse residues are removed through filter pressing of a plate frame, emulsified liquid is prepared through homogeneity and emulsification of a high-pressure homogenizer and directly packed into a paste or liquid feed, or is manufactured into a powder feed through absorption of extruded corns and drying of a tube bundle dryer, or is manufactured into a small-pellet feed through the drying of a spray dryer after tempering is performed by adding the water. The feed does not contain the fish meal and the plasma proteins, has the advantages of being high in nutrition, easy to digest, good in palatability, low in antinutritional factor content and the like, and can remarkably increase food consumption of piglets and stimulate the growth of the piglets.