40 results about "Saccharic acid" patented technology

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The invention discloses a genetically engineered bacterium for high yield of L-alanine as well as a construction method and application of the genetically engineered bacterium, a dominant strain is obtained by knocking out an alanine racemase gene dadX and integrating coding sequences of alanine dehydrogenase from different sources, and the shake flask yield is 10.8 g / L; the activity of a corresponding mutant M18L / E75A / A78S / I266V is 4.3 times that of a wild type; 6 by-product related genes are knocked out, meanwhile, a multi-copy strain integrated with alanine dehydrogenase is constructed, and the yield of E.coli A-LB6 in a 5L fermentation tank is increased to 133.3 g / L. Through a dynamic regulation and control strategy, an oxygen-sensitive promoter Pomp * is adopted to replace a phosphoenolpyruvate carboxylase promoter, so that succinic acid byproducts are reduced by 85.8%, and the yield reaches 143.5 g / L after 48 hours of aerobic and anaerobic two-stage fermentation. And finally, by regulating and controlling the ATP synthetase gene cluster atp promoter, the yield of L-alanine in a 50 L fermentation tank reaches 151.3 g / L, and the saccharic acid conversion rate reaches 95.2%. The combination of polygene knockout and dynamic metabolism regulation effectively enhances the biosynthesis ability of alanine, and provides an efficient engineering strain for industrial production.

A hydrogel for treating body cavity injury is derived from a chitosan derivative obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan by dehydration condensation. The hydrogel for treating the body cavity injury is granular, can be injected into a body cavity by utilizing an injector, and is used for treating the wound of the body cavity. A body cavity injury treatment kit is provided with a syringe, a tube connected to the syringe, and a granular body cavity injury treatment hydrogel filled into the syringe, the body cavity injury treatment hydrogel being derived from a chitosan derivative, the chitosan derivative is obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan through dehydration condensation.

The application uses cheap and easily available hexose acid as a starting material, first obtains two precursors of 2,5-furan dicarboxylic acid synthesis, namely hexahydroxy acid and 4-deoxy-5-dehydro-hexahydroxy acid through enzyme catalytic reaction, and then 4-deoxy-5-dehydro-hexahydroxy acid is dehydrated under acidic conditions to obtain 2,5-furan dicarboxylic acid.

The invention discloses a preparation method of ferrous L-threonate, and relates to the technical field of organic chemical synthesizing.The preparation method comprises the steps that vitamin C serves as a raw material and is oxidized through hydrogen peroxide to obtain threonic acid, residual hydrogen peroxide is removed through high temperature, and the threonic acid reacts with ferrous carbonate under the nitrogen protection condition to generate the ferrous L-threonate; and filtering and concentrating under reduced pressure to obtain a ferrous L-threonate crude product, and recrystallizing and purifying the crude product to obtain a ferrous L-threonate pure product. The process has the advantages of good process stability, high purity of pure products, cheap and easily available raw materials, simplicity in operation, high safety, environmental friendliness and the like.

The application discloses genetically engineered bacteria for synthesizing L-tryptophan and application thereof. The application realizes the following through two key gene regulation strategies (serA down-regulation and serB up-regulation): the accumulation amount of glutamic acid by-products is significantly reduced from 8-20 g / L to below 1.14 g / L, and the reduction ranges are 87.74% and 90.3% respectively; the sugar acid conversion rate (glucose / L-tryptophan) of L-tryptophan is increased from 19.2% to 20.9% and 21.3% respectively; without additional addition of expensive cofactors or inducers, the cost-effectiveness is significant.

The invention relates to the field of microorganisms, and discloses corynebacterium glutamicum and application thereof. Corynebacterium glutamicum with the preservation number of CGMCC No.35081 can tolerate the environment with the pH value of 5 and still has glutamic acid fermentation performance, the strain can still have high glutamic acid yield and sugar-acid conversion rate under the acidic condition with the pH value of 5.7-5.9, the performance of the strain is not reduced compared with that under the neutral fermentation condition, and even can be maintained at a considerable level, and the strain can be applied to the field of fermentation. By means of the characteristic, the adding amount of liquid ammonia in the later period of fermentation can be greatly reduced, and therefore raw material consumption and comprehensive production cost in the production process are remarkably reduced.

The invention discloses a double-target nerve conduction supporting nutritional composition and a preparation method thereof, and belongs to the technical field of nutritional products. The composition is prepared by compounding DHA algal oil, phosphatidylserine, EGCG and B vitamins with xanthoceras sorbifolia bunge oil nervonic acid and magnesium L-threonate which serve as dual-core targets in parts by mass, a'myelin sheath repair + signal transduction acceleration 'dual-target synergistic system is formed, membrane structure maintenance, oxidation resistance, inflammation resistance and neurotransmitter synthesis support are achieved at the same time, and the composition has the advantages of being simple in preparation process, low in cost and easy to popularize and apply. Neural conduction efficiency is systematically improved and instruction-execution latency is shortened. The problems of non-uniform mixing and caking of liquid oil and powder are solved through microencapsulation, the process is mature, the safety is high, and the stability is good. Animal experiments show that the composition can shorten the time of simple reaction by 30.5% (plt, 0.01) and shorten the time of selective reaction by 29.9% (plt, 0.01), has a non-obvious and unexpected technical effect, and is suitable for long-term nutritional supplement of middle-aged and elderly people and stroke convalescent people.

The application discloses a preparation method and application of chitooligosaccharide alduronic acid and belongs to the technical field of marine bioengineering. The preparation method comprises the following steps: after beta-chitin and a cleaving polysaccharide monooxygenase SmLPMO10 are dissolved in a Tris-hydrochloric acid buffer solution, a reducing agent is added, and an enzymatic reaction is carried out; after the reaction is carried out for 24 hours, the reducing agent is supplemented, the reaction is continuously carried out, the reducing agent is supplemented once every 24 hours, after the reaction is carried out for 96 hours, supernatant is obtained by filtration, and the chitooligosaccharide alduronic acid is obtained after inactivation, centrifugal removal of supernatant, desalination and freeze-drying. The chitin can be cleaved by using a single cleaving polysaccharide monooxygenase, without additional addition of chitinase, and the reaction condition is mild, so that the generation of by-products and environmental pollution are avoided. The application provides an environment-friendly preparation method of chitooligosaccharide alduronic acid, and the research and development of a green conversion technology of chitin and chitin-like biomass resources are greatly promoted.

Compositions and methods are described for improving brain health and cognitive function and for preventing and treating Alzheimer's disease and other age-related dementia diseases and conditions for intake as a dietary or nutritional supplement, for example, as a powdered drink mix. Compositions for providing an energy source to the brain during exercise are also described. The composition includes a source of choline, a source of magnesium, at least one taurine-containing composition, a source of ashwagandha root, and phosphatidylserine. The source of choline can be alpha-glycerylphosphorylcholine or citicoline and the source of magnesium can be magnesium-L-threonate or magnesium glycinate. The composition can further include at least one of oleic acid, oleanolic acid, ursolic acid, curcumin, piperine, galantamine, huperzine-A, a source of vitamin B5, L-tyrosine, and beta-hydroxybutyrate.

This present disclosure provides various diamine centered poly(amino acid)-saccharide-lipid conjugates. In various embodiments, the conjugates can safely be used as water solubility or bioavailability enhancers. In one aspect, the present disclosure provides a conjugate having the structural formula wherein B is a residue of a compound having three or four available binding positions, e.g., an alkylene diamine; S is a saccharide, for example, a residue of a mono-, di- or trisaccharide group, in which each saccharide unit is a sugar, a sugar alcohol, an amino sugar or a sugar acid; L is a lipophilic carrier residue selected from fatty acid residues (for example, residues of lauric acid, myristic acid, linoleic acid, palmitic acid, oleic acid and elaidic acid), steroid acid and sterol residues, and residues of retinoids carotenoids, tocopherols, and tocotrienols; Q is a residue of ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, pentaethylene glycol or hexaethylene glycol; P is a residue of a polyaminoacid homopolymer; R2 is a terminal group selected from H, methyl, ethyl and maleimide; D is a secondary group that is as defined above for L or is a methyl-capped poly(ethylene glycol) residue; and d is 0, or d is 1.

The invention relates to the field of microorganisms, and discloses recombinant escherichia coli, a construction method and application thereof and a method for producing L-leucine. The recombinant escherichia coli simultaneously contains a coding gene of an overexpressed pyruvic acid transport protein, a coding gene of an overexpressed glucose transport protein and a coding gene of an overexpressed 6-phosphofructokinase, and does not contain a coding gene of fructose-1, 6-diphosphatase. The recombinant Escherichia coli provided by the invention can significantly improve the utilization rate of the raw material glucose, improve the yield of L-leucine and inhibit the generation of byproducts. In a preferred embodiment of the invention, when the recombinant escherichia coli is fermented for 20 hours in a 5L fermentation tank, the yield of L-leucine reaches 70 g / L, the saccharic acid conversion rate reaches 36%, the daily yield is remarkably increased, and the production cost is greatly saved.

The invention relates to an alpha-glucosidase inhibitor and application thereof. The alpha-glucosidase inhibitor is vitamin C, dehydrogenated vitamin C, and 2, 3-diketone-L-gulonic acid. The influence of vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid on alpha-glucosidase and HepG2 cell glucose consumption is researched through an in-vitro model, and the result shows that the vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid can remarkably inhibit the activity of alpha-glucosidase and promote uptake and utilization of glucose by cells, so that the effect of improving insulin resistance is achieved.

An efficient method for producing L-threonic acid from L-xylonic acid is disclosed.

The application discloses a novel brown algae polysaccharide SARP for promoting healing of diabetic ulcers and a preparation and application thereof. A novel SARP is obtained through Sephacryl S-300 / HR gel column separation and purification, and the molecular weight of the SARP is about 45.4 kDa, which is composed of mannuronic acid (76.56%), guluronic acid (18.89%) and a small amount of glucuronic acid (4.55%). Compared with alginate separated from other brown algae, the SARP has a higher content of mannuronic acid. The SARP shows a better in-vitro antioxidant capacity, and can obviously reverse the cell damage of HUVECs caused by H2O2 and high sugar in the HUVECs. In addition, the SARP can promote the migration and tubular formation of the HUVECs. In-vivo experiments further show that the SARP can promote the healing of diabetic ulcer in rats, and can reduce the oxidative stress, inflammation level and lipid disorder in the rats. The SARP has a simple purification route, can be prepared in a large amount, and is a very promising novel drug for treating diabetic foot ulcers.

This invention discloses a method for the biosynthesis of high-purity L-threonate magnesium, belonging to the field of food fortification agents and biochemical synthesis technology. The invention uses L-xylorate calcium solution as a substrate, and obtains a purified L-threose solution through oxidative cleavage. Then, a complex dehydrogenase system composed of arabinose dehydrogenase, xylose dehydrogenase, and aldose dehydrogenase synergistically catalyzes the oxidation of L-threose to L-threonate. Finally, L-threonate magnesium is prepared by salt formation with magnesium hydroxide, crystallization, and drying. The purity of the obtained product is greater than 99.0%, the oxalic acid residue is less than 0.01%, the process is safe and environmentally friendly, and suitable for industrial production.

The invention belongs to the technical field of agricultural hydrogel development, and particularly relates to hydrogel for slowly releasing plant biostimulant as well as a preparation method and application of the hydrogel. The hydrogel is obtained by crosslinking the polyglutamic acid and the 2-keto-L-gulonic acid fermentation liquor. The hydrogel disclosed by the invention is relatively high in mechanical strength, has relatively good adsorption performance on soil moisture and shows good water storage and retention capacity, so that the use amount of irrigation water is effectively reduced; besides, the hydrogel can continuously release glutamic acid, 2-keto-L-gulonic acid, D-gulonic acid-gamma-lactone, citric acid and other micromolecular organic matters with the plant biostimulant function in the soil microenvironment, the content of quick-acting organic nutrients in soil is effectively increased while the water retention effect is achieved, the microbial activity is enhanced, and the soil remediation effect is improved. The soil conditioner can improve crop rhizosphere soil and strengthen the synergistic effect of water and fertilizer, thereby effectively promoting crop growth, yield increase and vitamin C accumulation, and has good application value in the fields of agriculture and environment.

This invention relates to a method for preparing fermented deer antler extract, a fermented deer antler extract prepared by the above method, and a method for increasing the content of sulfated glycosaminoglycans, N-acetylneuraminic acid, and free amino acids in the fermented deer antler extract. The method for preparing fermented deer antler extract is characterized by comprising: step (1) treating pulverized deer antler under high temperature and pressure conditions; step (2) adding a complex enzyme of protease, cellulase, β-glucanase, and hemicellulase to a deer antler liquid containing water treated in step (1) for reaction; step (3) sterilizing the deer antler liquid reacted in step (2), inoculating the sterilized deer antler liquid with Bacillus coagulans culture for fermentation, and then filtering; and step (4) sterilizing the filtrate filtered in step (3), concentrating, and drying it.

The invention belongs to the field of food processing, and particularly relates to application of a sulfurous acid-ascorbic acid synergistic system to increase hardness of hawthorn pectin with high gel property, pectin and application. According to the technical scheme, the method comprises the following steps: adding L-ascorbic acid and sulfurous acid into a hawthorn powder suspension to obtain a mixed solution, adjusting the pH value of the mixed solution to be 1.5-2.5 by using sulfurous acid, heating and extracting the mixed solution in a closed container, cooling and centrifuging the heated and extracted mixed solution, taking supernate, adding absolute ethyl alcohol, standing, taking precipitate, washing, and drying to obtain the hawthorn extract. The hawthorn pectin is obtained. The method can prevent easily oxidized polyphenol substances and procyanidine in the hawthorn from being oxidized to form quinones compounds to influence the pectin quality in the hawthorn pectin extraction process, can improve the pectin gel hardness, and improves the reduction effect of sulfurous acid in the pectin preparation process.

The present disclosure provides PEG-saccharide-lipid conjugates centered around various diamines. In various embodiments, these conjugates can be safely used as water-soluble or bioavailability enhancers. In one aspect, the present disclosure provides a PEG-saccharide-lipid conjugate having the following structural formula. JPEG2026528703000080.jpg27170Here The number average value of m ranges from 2 to 10. S is a monosaccharide, disaccharide or trisaccharide group, and each sugar unit is a sugar, sugar alcohol, amino sugar or sugar acid. L is -C(O)-R 1 where R 1 is an alkanoyl or alkenoyl group having an average carbon number of 6 to 22, and / or a steroid acyl group. P is -(CH2-CH2-O)nR 2 where "n" has a number average value in the range of 5 to 50 (e.g., 8 to 45), and R 2 is hydrogen and / or an alkane and has a number average carbon number in the range of 0 to 4.

The present invention relates to a composition for caring for keratinous materials, comprising: (i) at least one hydroxyl-containing acid selected from the group consisting of α-hydroxyl acids, gluconic acid, lactobionic acid, salicylic acid, precursors thereof, and mixtures thereof; (ii) at least one short-chain surfactant, each lipophilic chain of which contains 14 or fewer carbon atoms; (iii) at least two fatty alcohols having 20 or more carbon atoms; (iv) at least one non-fatty alcohol surfactant other than the short-chain surfactant; The present invention relates to a composition comprising:

The invention provides a glycosyltransferase high-throughput screening method based on p-nitrophenol, and belongs to the technical field of biology. According to the method, yellow p-nitrophenol disappears after glycosylation is carried out on yellow p-nitrophenol to form glycoside, and the change of absorbance is measured at 400 nm through an ultraviolet-visible spectrophotometer or a microplate reader so as to determine the conversion amount of p-nitrophenol, so that the enzyme activity of glycosyltransferase is measured. Compared with the existing glycosyl transferase activity determination and screening method, the method disclosed by the invention does not need expensive equipment such as liquid chromatography, flow cytometry and the like, and does not need expensive fluorescent substrates, radioactive nucleotide saccharic acid, antibodies and the like. The method has the characteristics of simplicity and convenience in operation, rapidness, high flux and the like.

The invention provides a non-distillative process for manufacturing amphetamine and substituted amphetamines, comprising obtaining a highly pure phosphoramidate compound, converting the highly pure phosphoramidate compound to an amphetamine sulfate compound, concentrating the amphetamine sulfate compound in isopropanol, and then salting out the amphetamine compound directly to obtain an amphetamine salt, the amphetamine salt selected from amphetamine saccharate, amphetamine sulfate, amphetamine aspartate, alkyl-amphetamine saccharate, alkyl-amphetamine sulfate, alkyl-amphetamine aspartate, aryl-amphetamine saccharate, aryl-amphetamine sulfate, aryl-amphetamine aspartate, and mixtures thereof.

This invention belongs to the field of biodegradable material modification, specifically relating to a basic magnesium sulfate whisker-reinforced polylactide composite, its preparation method, and its applications. The basic magnesium sulfate whisker-reinforced polylactide composite of this invention comprises the following components by mass fraction: 80-97.5% gluconolactone-modified polylactide and 2.5-20% basic magnesium sulfate whiskers. The basic magnesium sulfate whisker-reinforced polylactide composite provided by this invention possesses high modulus and rigidity, exhibiting early defect support and ligation fixation functions. It gradually degrades and is absorbed and replaced by tissue. The degradation components provide various organic components that promote tissue regeneration, enhancing tissue regeneration and reconstruction. Its degradation rate can be controlled according to the composition to match tissue regeneration and reconstruction. During use, it reduces surgical infections caused by bacterial infection and inflammation, such as redness and swelling, and exudation. It has wide applications in surgical ligation fixation, defect repair, short-term fixation, and isolation.

The application relates to the field of biomass conversion, and discloses a noble metal catalyst, a preparation method and application thereof, and a preparation method of a biomass-based carboxylic acid polymer monomer. The catalyst comprises a carrier and an active component loaded on the carrier, the active component comprises noble metal and rhenium, and the carrier is a carbon nanotube; wherein the dispersion degree of the noble metal is 40-60%. The noble metal catalyst has the advantages that the dispersion degree of the noble metal is high, the hydrogen reduction condition is mild, the catalyst is used for preparing adipic acid from saccharic acid under a water system, the saccharic acid conversion rate is high, the selectivity of the adipic acid is high, and the reaction is green and environment-friendly.

The invention belongs to the technical field of biology, and particularly relates to an L-isoleucine high-yield strain and application thereof. The L-isoleucine high-yield strain is obtained through molecular modification, mutagenesis and resistance screening, and compared with an original strain, the fermentation yield of L-isoleucine and the saccharic acid conversion rate are greatly increased, so that the production cost can be reduced, and the strain has practical value.

COSMETIC COMPOSITION FOR THE CARE OF KERATINIC MATERIALS The present invention relates to a composition for the care of keratinous materials, comprising (i) at least one hydroxyl-containing acid, selected from the group consisting of α-hydroxy acids, gluconic acid, lactobionic acid, salicylic acid, their precursors and mixtures thereof; (ii) at least one short-chain surfactant, each oleophilic chain containing 14 carbon atoms or fewer; (iii) at least two fatty alcohols having 20 carbon atoms or more; and (iv) at least one non-fatty alcoholic surfactant other than said short-chain surfactant. Figure for the abstract: none

A problem addressed is to provide a useful means for increasing muscle mass. A composition containing threonic acid, one or more bacteria selected from Bifidobacterium spp., Lactobacillus spp., Lacticaseibacillus spp., Lactiplantibacillus spp., Limosilactobacillus spp., Levilactobacillus spp., Ligilactobacillus spp., and Latilactobacillus spp., and one or more selected from cultures of the aforementioned bacteria is used to increase muscle mass. Such a composition is produced through, e.g., a method that includes a step for culturing one or more bacteria selected from the aforementioned bacteria in ascorbic-acid-containing medium and a step for recovering a threonic-acid-containing fraction from the culture after culturing.