Fermentation production method for citric acid

A production method and technology of citric acid, which is applied in the field of continuous fermentation production of citric acid, can solve the problems of unstable batch fermentation production, large fluctuations in fermentation results, and low utilization rate of fermentation tanks, so as to shorten the fermentation cycle and improve conversion rate, the effect of improving the utilization rate

Active Publication Date: 2011-11-23
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the non-fermentation time, that is, the auxiliary fermentation time takes a long time, the utilization rate of the fermentation tank is not high, and the batch fermentation production is unstable, and the fermentation results between batches fluctuate greatly.

Method used

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  • Fermentation production method for citric acid
  • Fermentation production method for citric acid

Examples

Experimental program
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Effect test

Embodiment 1

[0041] at 50m 3 Add seed medium containing 12.5% ​​total sugar (m / m) and Aspergillus niger spores into the seed tank, maintain pH 3-7, temperature 40°C, and cultivate for 12-24 hours to form Aspergillus niger balls for use.

[0042] Add corn fermentation medium containing 12.5% ​​(m / m) of total sugar to 250m 3 In the first-stage fermenter of the tank capacity, when the fermentation medium accounts for 70% of the volume of the fermenter, the ventilation, stirring and cooling systems are activated to maintain the temperature of the material liquid in the fermenter at 35-37°C and inoculate according to 10% of the inoculation amount. Inject Aspergillus niger balls from the seed tank and maintain an air volume of 8.6 m 3 / m 3 h, pH2.9-4.5;

[0043] According to 13.5m 3 The flow rate of / h continuously adds the fermentation medium and the Aspergillus niger balls from the seed tank at a rate of 10% to the primary fermenter, according to 14.85m 3 The flow rate of / h flows out fro...

Embodiment 2

[0049] at 50m 3 Add seed medium containing 13.0% total sugar (m / m) and Aspergillus niger spores into the seed tank, maintain pH 3-7, temperature 38°C, and cultivate for 12-24 hours to form Aspergillus niger balls for use.

[0050] Add corn fermentation medium containing 13.5% (m / m) of total sugar to 250m 3 In the first-stage fermenter of the tank capacity, when the fermentation medium accounts for 80% of the volume of the fermenter, the ventilation, stirring and cooling systems are started to maintain the temperature of the feed liquid in the fermenter at 35-37°C and the inoculation amount of 8% is inoculated. Inject Aspergillus niger balls from the seed tank and maintain an air volume of 9.8 m 3 / m 3 h, pH2.9-4.5;

[0051] Press 13m 3 The flow rate of / h is continuously added to the primary fermenter with fermentation medium and aspergillus niger pellets from the seed tank at a rate of 8%, 14.85m 3 The flow rate of / h flows out from the primary fermentation tank or extra...

Embodiment 3

[0057] at 75m 3 Add seed medium containing 12.5% ​​total sugar (m / m) and Aspergillus niger spores into the seed tank, maintain pH 3-7, temperature 40°C, and cultivate for 12-24 hours to form Aspergillus niger balls for use.

[0058] Add cassava fermentation medium containing 12.5% ​​(m / m) of total sugar to 550m 3 In the first-stage fermenter of the tank capacity, when the fermentation medium accounts for 75% of the volume of the fermenter, the ventilation, stirring and cooling systems are started to maintain the temperature of the material liquid in the fermenter at 35-37°C and the inoculation amount is 10%. Inject Aspergillus niger balls from the seed tank and maintain an air volume of 8.5 m 3 / m 3 h, pH2.9-4.5;

[0059] Press 31m 3 The flow rate of / h is continuously added to the primary fermentation tank with fermentation medium and aspergillus niger balls from the seed tank at a rate of 10%, according to 34.1m 3 The flow rate of / h flows out from the primary fermentat...

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Abstract

The invention discloses a fermentation production method for citric acid, and in particular relates to a continuous fermentation production method for citric acid. A citric acid continuous fermentation system consists of a culture medium storage tank, a seed culture tank, two or more serially connected fermentation tanks and a fermentation mash storage tank. By the citric acid continuous fermentation system, a fermentation period can be saved, the conversion rate of saccharic acid and the utilization rate of the fermentation tanks are improved, and stages such as growth and development and saccharification effects of aspergillus niger on citric acid production bacteria, fermentation and acid production and the like are controllable sectionally.

Description

technical field [0001] The invention belongs to a production method of citric acid, in particular to a continuous fermentation production method of citric acid. Background technique [0002] Citric acid is one of the most used organic acids in the world today. So far, the citric acid consumed in the world is mainly produced by the fermentation method with Aspergillus niger as the strain. The existing citric acid fermentation technology adopts the intermittent batch fermentation mode. Every time a batch of fermentation is completed, it is necessary to discharge and clean, sterilize, feed and re-inoculate the fermenter before proceeding to the next batch. fermentation. As a result, the non-fermentation time, that is, the auxiliary fermentation time, takes a long time, the utilization rate of the fermentation tank is not high, and the batch fermentation production is unstable, and the fermentation results between batches fluctuate greatly. Contents of the invention [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12R1/685
Inventor 寇光智楼良旺李昌涛丁兆堂张灵燕
Owner RIZHAO JINHE BOYUAN BIOCHEM
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