54 results about "Neuraminic acid" patented technology

The invention relates to the technical field of biology, and particularly discloses phosphatidylserine nervonic acid gel candies capable of improving concentration and a preparation method of the phosphatidylserine nervonic acid gel candies. The invention relates to a phosphatidylserine nervonic acid gel candy for improving concentration. Comprising the following raw materials in parts by weight: 50 to 200 parts of phosphatidylserine, 10 to 30 parts of cis-15-tetracosenoic acid, 1 to 10 parts of a balm extract, 1 to 10 parts of a salvia officinalis extract, 1 to 10 parts of N-acetylneuraminic acid, 1 to 10 parts of tea theanine, 100 to 400 parts of medium chain triglyceride, 5 to 40 parts of beeswax, 5 to 40 parts of phospholipid and the balance of purified water. The composition can be used for improving concentration and has the advantages of being good in stability, good in taste and high in acceptability.

The invention aims to disclose a special dietary preparation based on alpha-ketoglutaric acid as well as a preparation process and application thereof, and relates to the technical field of dietary supplements. Every 800 mg of the dietary preparation comprises 50 mg to 100 mg of alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, 5 mg to 12 mg of N-acetylneuraminic acid or N-acetylneuraminic acid salt, 30 mg to 60 mg of ergothioneine, 100 mg to 150 mg of a desert cistanche deserticola water extract or 50 mg to 80 mg of desert cistanche deserticola total glycosides, and auxiliary materials. The alpha-ketoglutaric acid salt is any one or more of alpha-ketoglutaric acid calcium, alpha-ketoglutaric acid sodium and alpha-ketoglutaric acid magnesium; the N-acetylneuraminic acid salt is any one or more of N-acetylneuraminic acid calcium, N-acetylneuraminic acid sodium and N-acetylneuraminic acid magnesium; the special dietary preparation has the beneficial effects that the special dietary preparation simultaneously contains alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, N-acetylneuraminic acid or N-acetylneuraminic acid salt, ergothioneine and the cistanche deserticola aqueous extract, and has the effects of delaying senescence, resisting fatigue, improving the symptom of kidney-yang deficiency and enhancing immunity for males aged more than 45 years old.

The invention discloses recombinant escherichia coli with high yield of N-acetylneuraminic acid and application of the recombinant escherichia coli, and relates to the technical field of biological genetic engineering. The invention relates to a recombinant escherichia coli, which is characterized in that the escherichia coli is taken as a host, and free expression of an N-acetylmannosamine epimerase gene yihS from Streptomyces xiamenensis or an N-acetylmannosamine epimerase gene ce3 from Bacteroides polymorpha and an exogenous N-acetylneuraminic acid lyase gene nano A is carried out; and carrying out recombinant expression on N-acetyl hexosamine 1-kinase nahK, a UDP-N-acetyl glucosamine pyrophosphorylase gene glmU and a UDP-N-acetyl glucosamine-2-epimerase gene neuC in the other synthetic route of the ManNAc. According to the recombinant escherichia coli with high yield of N-acetylneuraminic acid, the yield of N-acetylneuraminic acid can reach 23.08 g / L under a shake flask fermentation condition; a two-stage batch feeding strategy is adopted, the yield of N-acetylneuraminic acid in a 5L fermentation tank reaches 71.25 g / L, the molar conversion rate of GlcNAc reaches up to 57.60%, and the method has the potential of industrial application.

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for producing N-acetylneuraminic acid as well as construction and application of the genetically engineered bacterium. Accumulation of acetic acid is controlled by combining and applying different strategies: rational transformation is performed on an escherichia coli metabolic pathway, and acetic acid synthesis pathway related genes poxB, ackA and pta are knocked out to reduce acetic acid synthesis; the glyoxylic acid cycle is activated by knocking out the coding gene iclR of the glyoxylic acid cycle transcriptional regulation factor, and acetic acid overflow is reduced; the fermentation temperature is adjusted to control overall metabolism of cells to reduce acetic acid accumulation. Through comprehensive application of the strategy, the Neu5Ac production method capable of effectively controlling acetic acid accumulation is obtained, and the method has a good industrial application prospect.

The invention relates to nervonic acid-loaded near-infrared light response type injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological engineering. According to the invention, P (NIPAM-co-NHMAM) and a modified natural polymer material are combined to prepare a temperature-responsive hydrogel capable of realizing reversible volume shrinkage / expansion at a specific temperature, near-infrared light physiotherapy and irradiation are combined to damage local skin, and the hydrogel is stimulated to realize reversible volume deformation by controlling tissue temperature, so that the temperature-responsive hydrogel can be used for preparing the hydrogel with the reversible volume shrinkage / expansion at the specific temperature. Therefore, accurate and wireless space-time regulation and control of nervonic acid release behaviors are realized. Therefore, the nervonic acid loaded near-infrared light response type injectable hydrogel disclosed by the invention can realize efficient carrying and light response controllable release of nervonic acid, promotes functional recovery after spinal cord injury, and has a relatively good application prospect and research significance.

The invention relates to an N-acetylneuraminic acid fermentation method capable of reducing fermentation byproducts, and belongs to the technical field of fermentation. Escherichia coli is used as a chassis strain for fermentation synthesis of N-acetylneuraminic acid, sodium hexametaphosphate is added in the fermentation process to reduce generation of byproducts in the synthesis process of N-acetylneuraminic acid, and fermentation conditions are further optimized to improve the synthesis yield of N-acetylneuraminic acid. According to the fermentation method, the N-acetylneuraminic acid can be efficiently synthesized, the yield of the N-acetylneuraminic acid is increased, meanwhile, by-products difficult to separate are reduced, and a technical basis is provided for subsequent fermentation amplification and industrial production of the N-acetylneuraminic acid.

The present invention relates to a method for producing α-sialyl-β-D-galactoside saccharides, particularly α-sialyl-(2→3)-β-D-galactoside saccharides and α-sialyl-(2→6)-β-D-galactoside saccharides, from a β-D-galactoside saccharide, a sialic acid donor, and an enzyme with β-galactoside α-sialyltransferase activity. The enzymes with β-galactoside α-sialyltransferase activity used herein do not exhibit catalytic activity towards the hydrolysis of cytidine 5'-monophospho-N-acetyl-neuraminic acid to cytidine and N-acetyl-neuraminic acid. The method can be performed in vitro and in vivo using a genetically engineered cell comprising a nucleic acid encoding said enzyme. Further, said process may be adapted to produce the sialic acid donor CMP-Neu5Ac from low-cost substrates N-acetyl-D-glucosamine (GlcNAc), pyruvate, a cytidine phosphate (CMP, CDP or CTP) and polyphosphate in a single reaction mixture with a set of optionally immobilized or optionally co-immobilized enzymes comprising N-acylglucoamine 2-epimerase (AGE), an N-acetylneuraminate lyase (NAL), an N-acylneuraminate cytidylyltransferase (CSS), optional a uridine kinase (UDK), a uridine monophosphate kinase and a polyphosphate kinase 3 (PPK3).

This invention relates to the field of cosmetic technology, specifically disclosing a synergistic exfoliating composition and a method for preparing a controlled-release carrier containing the composition. The synergistic exfoliating composition is a compound of adenosine, N-acetylneuraminic acid, and lactobionic acid. This invention has the advantage of maintaining good exfoliating efficacy while reducing skin irritation.

The invention belongs to the field of gene engineering, and particularly relates to an N-acetyl glucosamine isomerase mutant, a recombinant expression vector, a recombinant strain and application thereof. According to the invention, modification is carried out based on amino acid of wild type N-acetyl glucosamine isomerase from Anabaena sp.CH1, the modified mutant comprises two mutation sites, namely K84D and F349Y, and the activity is improved by 1.84 times. The invention further constructs a double-enzyme co-expression strain of N-acetylglucosamine isomerase and N-acetylneuraminic acid aldolase, and the strain is used for producing N-acetylneuraminic acid, so that the dosage of thalli is reduced, the conversion efficiency is improved, a more economical enzyme catalysis scheme is provided for industrial catalytic preparation of N-acetylneuraminic acid, and the method has a wide application prospect. The method has an important industrial application prospect.

The invention relates to the technical field of nervonic acid extraction, in particular to a device and method for separating nervonic acid from malania oleifera oil for biological medicine manufacturing, the device comprises a distiller, the distiller comprises a sealing tank and a dismounting cylinder, and a film scraping mechanism and a plurality of negative pressure tanks are arranged in the sealing tank and the dismounting cylinder respectively. According to the device and method for separating nervonic acid from malania oleifera oil for biological medicine manufacturing, a spiral scraping plate of a lifting part is linked with a reciprocating lead screw through a clamping ring, synchronous rotation and up-down reciprocating motion are achieved, the whole surface of a condensation pipe is covered, a condensate liquid film is actively scraped away, heat resistance of a retained liquid film is eliminated, the condensation efficiency is improved, and the efficiency of separation of nervonic acid from malania oleifera oil is improved. The nervonic acid fraction is prevented from staying on a condensation wall for a long time, and the condition of thermal degradation or backmixing is prevented.

The invention relates to the technical field of food science and analytical chemistry, in particular to a method for analyzing carbohydrates in sialic acid lactose and application of the method. The analysis method disclosed by the invention comprises the following steps: separating carbohydrates through ultra-high performance liquid chromatography, wherein in the ultra-high performance liquid chromatography, a mobile phase A comprises acetonitrile; the mobile phase B comprises 10-30 mmol / L of an ammonium formate aqueous solution; the elution procedure is a specific gradient elution procedure. According to the analysis method, lactose, N-acetylneuraminic acid, 3 '-sialic acid lactose, 6'-sialic acid lactose, 3 '-sialic acid lactulose, 6'-sialic acid lactulose and other carbohydrates in sialic acid lactose can be completely separated, and the problem that the substances cannot be effectively separated in the prior art is solved.

For the purpose of determination for the selection of intravesical BCG instillation therapy, the present invention comprises measuring 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitine, lauric acid, myristic acid, octanoylcarnitine, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acid, pentanoic acid, 1-methyl-4-imidazoleacetic acid, ADMA, alanine, argininosuccinic acid, choline, citrulline, creatinine, guanidoacetic acid, lysine, methionine sulfoxide, myristic acid, N-(1-deoxy-1-fructosyl)leucine, N-acetylneuraminic acid, N6,N6,N6-trimethyllysine, propionylcarnitine, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, and gamma-glutamyl-valine in blood, serum, or plasma.

The application relates to the technical field of medicines, in particular to application of N-acetylneuraminic acid in preparation of medicines for preventing and / or treating ulcerative colitis. The application finds that N-acetylneuraminic acid can effectively prevent or treat ulcerative colitis and promote tissue repair by regulating macrophage metabolism, inhibiting glycolysis pathways and M1 type polarization, and simultaneously repairing intestinal mucosal barrier, thereby providing a safe, simple and clear mechanism of action new treatment strategy for clinic.

The present invention relates to the field of genetic engineering technology, and in particular to an engineered Escherichia coli strain that produces N-acetylneuraminic acid, as well as its construction method and application. Based on existing engineered bacteria, the present invention further optimizes the metabolic pathway from glucose to N-acetylmannosamine and knocks out the glmM gene to reduce the production of non-related metabolites. The resulting genetically engineered bacteria significantly enhance the Escherichia coli's ability to produce Neu5Ac in fermentation experiments, demonstrating promising prospects for industrial fermentation applications.

The invention provides a preparation method of an antibody conjugate containing an sLeX tetrasaccharide structure, which comprises the following steps: S1, sequentially adding Endo S2 enzyme and alpha-L-fucosidase into a target antibody, and respectively analyzing glycosylation to avoid modification site conflict or carbohydrate chain structure interference; s2, adding the accurately represented antibody in the S1, B4GalT1 and UDP-Gal into a Tris-HCl system containing Mn < 2 + >, and incubating to obtain a reaction solution I; s3, the reaction liquid I, ST3Gal3, CMP-Neu5Ac and Tris-HCl containing Mg < 2 + > are taken and mixed, and incubation is carried out; adding CMP-N-acyl neuraminic acid phosphodiesterase for reaction, and purifying Protein A to obtain a purified antibody I; s4, the purified antibody I, alpha1, 3 FucT-2HR, GDP-Fuc and Tris-HCl containing Mg < 2 + > are taken and mixed, and incubation is performed; and purifying Protein A to obtain a target product. According to the modified antibody obtained by the invention, while the targeting property is improved, the binding affinity of the antibody and a tumor cell surface antigen can be enhanced, so that the immune blocking activity of the antibody is further enhanced, and dual improvement of the targeting property and the activity is realized.

The invention discloses a GlycoRNA detection platform of a nonlinear hybrid chain reaction circuit based on proximity coding and application of the GlycoRNA detection platform, the GlycoRNA detection platform is composed of a recognition module and an assembly module, the recognition module comprises a glycan probe for triggering a nonlinear HCR reaction and an RNA specific probe, the glycan probe comprises an aptamer for selectively binding N-acetylneuraminic acid, a spacer region, a binding region and a trigger region, the RNA specific probe contains a DNA (Deoxyribose Nucleic Acid) sequence complementary with an RNA sequence of GlycoRNA, a spacer region, a hybridization region and a trigger region; the assembly module comprises two double-stranded substrates and two single-stranded assistants; each double-stranded substrate is composed of a DNA chain marked by a quenching agent and a DNA chain marked by a fluorophore. The method does not need enzyme, solves the actual limitation related to enzymatic reaction, saves time, simplifies the process, and has high sensitivity and high specificity.

The invention discloses an application of ionic liquid functionalized super-crosslinked resin in nervonic acid adsorption. The imidazole functionalized super-crosslinked resin HCl-M has high specific surface area and optimized pore size distribution, is matched with nervonic acid molecular size, remarkably improves the adsorption capacity and selectivity of nervonic acid, enhances the interaction force between the resin and nervonic acid through ionic liquid functional modification, and further improves the adsorption performance. The adsorption capacity of the imidazole functionalized super-crosslinked resin to nervonic acid is remarkably improved, and the adsorption process is controlled by multiple mechanisms such as screening action, hydrogen bond and pi-pi interaction and the like. The invention provides theoretical guidance and technical support for efficiently adsorbing nervonic acid from shiny-leaved yellowhorn oil, and has important application value.

The present invention relates to a novel and efficient method for sialylation of a glycoside comprising mixing said glycoside with sialic acid, cytidine monophosphate, nucleoside triphosphate and one or more cell-free extracts of a microorganism, the present invention relates to a microorganism comprising one or more endogenous polypeptides having inorganic diphosphatase activity and one or more endogenous polypeptides having phosphotransferase activity, and wherein the one or more cell-free extracts comprise: at least one polypeptide having cytidine monophosphate kinase activity, at least one polypeptide having N-acyl neuraminic acid cytidyltransferase activity, and at least one polypeptide having sialyltransferase activity, thereby sialylating the glycoside.

The application discloses an immunity-enhancing composition, a freeze-dried composition, a freeze-dried milk block and a preparation method thereof, and belongs to the field of food. The immunity-enhancing composition is prepared by reasonably compounding lactoferrin, N-acetylneuraminic acid, PQQ, oat beta-glucan and taurine, fully volatilizing the synergistic effect among the components, and has excellent immunity-enhancing effect on the body. The immunity-enhancing composition can be used for preparing freeze-dried food such as freeze-dried milk blocks and freeze-dried powder, and can endow the food with additional functions and help to improve the added value of the food and develop new markets.

The invention discloses a method for quantitatively analyzing the content of alpha2-3 acetylneuraminic acid in glycoprotein, which comprises the following steps: firstly, treating a sample containing glycoprotein by using streptococcus pneumoniae acetylneuraminidase, and continuously treating the sample containing glycoprotein by using clostridium perfringens acetylneuraminidase in the same solution; centrifugally separating and taking a separating solution containing two acetylneuraminic acid products outside the centrifugal tube; and detecting and collecting two hydrolysates 2, 7-dehydrated acetylneuraminic acid and the signal intensity of acetylneuraminic acid in a negative ion mode, and calculating the percentage content of alpha2-3 linked acetylneuraminic acid by using a formula. According to the method, the sensitivity of quantitative analysis of alpha2-3 acetylneuraminic acid can be improved, and the time consumption of detection and analysis is reduced.

Disclosed are methods for the fermentative production of a sialylated saccharide and genetically engineered microbial cells for use in said method, wherein the genetically engineered microbial cells comprise (i) a sialic acid biosynthesis pathway comprising a glucosamine-6-phosphate N-acetyltransferase, (ii) a cytidine 5′-monophospho-(CMP)-N-acetylneuraminic acid synthetase; and (iii) a sialyitransferase, for producing sialylated saccharides, as well as the use of said sialylated oligosaccharides for providing nutritional compositions.

The present invention addresses the problem of providing a complex for obtaining a monoclonal antibody that specifically recognizes both a sugar chain and a peptide constituting a glycopeptide as a dynamic epitope at the same time. The present invention relates to a complex represented by formula (1). In formula (1), R1 is a neuraminic acid group or a neuraminic acid derivative group, DE is an antigenic site selected from glycoproteins and glycopeptides, CP is a vector site, and X1 and X2 are each independently a single bond or a linking group.

The invention relates to an elsholtzia and ginseng composite peptide special diet for improving cardiovascular and cerebrovascular diseases and microcirculation disturbance and a preparation method thereof. The special diet comprises the following components: 5-15% of small molecule peptide; 18-65% of a plant extract; 0.1 to 3 percent of gamma-aminobutyric acid; 0.01 to 1 percent of N-acetylneuraminic acid; 0.005% to 0.1% of thiamine; 0.001 to 0.05 percent of riboflavin; and the balance of auxiliary materials. Wherein the plant extract comprises at least one of elsholtzia, rhizoma zingiberis and ginseng. According to the scheme provided by the invention, the elasticity and permeability of blood capillaries can be improved, microcirculation can be improved, blood fat can be reduced, and cardiovascular and cerebrovascular diseases can be effectively prevented and relieved.

The invention relates to the technical field of biological medicines, in particular to application of N-acetylneuraminic acid in preparation of a medicine for treating methamphetamine addiction. The key effect of N-acetylneuraminic acid (Neu5Ac) in methamphetamine addiction treatment is found for the first time, the CPP score of a methamphetamine addiction mouse can be obviously reduced, it is proved that the N-acetylneuraminic acid (Neu5Ac) has a good treatment effect on methamphetamine addiction, and Neu5Ac can be subjected to endogenous synthesis and has the advantages of being good in biocompatibility and small in toxic and side effect.

The invention discloses a composite peptide product with functions of improving sleep and brain health and a preparation method thereof, and relates to the field of health food, the composite peptide product comprises the following components in percentage by mass: a functional peptide group: 5-20% of walnut oligopeptide, 0.5-5% of casein phosphopeptide, 2-10% of collagen peptide, 0.5-2% of spina date seed peptide and 0.5-2% of cattle spleen peptide; a nerve activity group: 0.1%-6% of phosphatidylserine, 0.5%-5% of N-acetylneuraminic acid and 0.1%-5% of tea leaf theanine; a vitamin group, a carrier matrix and an auxiliary material group; according to the composite peptide product with the functions of improving sleep and brain health and the preparation method of the composite peptide product, the functional peptide group, the nerve activity group, the vitamin group, the carrier matrix and other components are scientifically proportioned, so that the limitation of a single active component is overcome, and the overall bioavailability and the targeted release capacity of the product are also remarkably improved.

Provided herein is a DNA-based nanostructure at least a first plurality of virus-binding moieties, wherein each virus-binding moiety of the at least first plurality of virus-binding moiety is neuraminic acid or a derivative thereof. Also provided herein is a self-assembling DNA-based building block formed by a single-stranded DNA template strand and a set of oligonucleotides complementary to said single-stranded DNA template, wherein: each of the oligonucleotides is either complementary to one contiguous DNA sequence stretch or to at least two non-contiguous DNA sequence stretches on said single-stranded DNA template; and the self-assembling DNA-based building block comprises at least one first virus-binding moiety, wherein the first virus-binding moiety is neuraminic acid or a derivative thereof. Also provided herein is a DNA-based nanostructure wherein the DNA-based nanostructure comprises at least five self-assembling DNA-based building blocks; each of the self-assembling DNA-based building blocks is formed by a single-stranded DNA template strand and a set of oligonucleotides that are at least partially complementary to said single-stranded DNA template; and wherein each of the oligonucleotides is either complementary to one contiguous DNA sequence stretch or to at least two non-contiguous DNA sequence stretches on said single-stranded DNA template. Also provided herein are associated methods, uses, compositions, and kits.

The invention provides an anti-aging composition containing collagen and application of the anti-aging composition. The anti-aging composition containing the collagen is prepared from the following components in percentage by mass: 2 to 5 percent of protein substances, 0.05 to 0.2 percent of aldehyde compounds, 4 to 6 percent of N-acetylneuraminic acid, 0.5 to 2 percent of retinol substances, 1 to 3 percent of phosphatidylcholine and the balance of a basic matrix. The collagen-containing anti-aging composition prepared by the invention has the advantages that the surface wrinkle area reduction amount is obviously reduced, the wrinkle fading period is obviously shortened, the skin resilience is good, the DPPH free radical scavenging rate is higher, the skin surface wrinkles can be obviously reduced after long-term use, the skin resilience is improved, the wrinkle fading period is short, and the anti-aging effect is good. The antioxidant effect and the anti-aging effect are better.

This invention relates to the field of cosmetic technology, disclosing the application of N-acetylneuraminic acid in synergistically enhancing the skincare effects of acetyl hexapeptide-8. By combining N-acetylneuraminic acid and acetyl hexapeptide-8 in the mass ratios provided in this application, a synergistic effect on the anti-aging effects of acetyl hexapeptide-8 can be achieved, increasing the content of collagen and elastin in the skin. However, when the ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is adjusted outside the range provided in this application, the synergistic effect disappears. Therefore, by using N-acetylneuraminic acid and acetyl tetrapeptide-2 synergistically in cosmetics and / or skincare products according to the compounding ratios provided in this application, significant anti-aging effects can be achieved at lower application amounts, improving skin firmness and elasticity, and reducing the formation of wrinkles.

The present invention relates to a novel AGEs-decomposing agent, which is characterized by containing a compound selected from the group consisting of a rose cv.Plena extract, an Acetylneuraminic acid (NANA, Acetylneuraminic acid), a Camellia japonica flower extract, a Crocus sativus L. extract, a Citrus aurantium L. powder, a nicotinamide mononucleotide (NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN The invention relates to a compound which is prepared from at least one of the following raw materials: an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae and an extract of radix