The invention discloses a tortoise jelly with sweet aftertaste and elasticity and a preparation method thereof. The tortoise jelly comprises the following raw materials in parts by weight: 30000-50000 parts of water, 1000-1500 parts of tortoise jelly powder, 800-1200 parts of mauremys mutica, 80-120 parts of chrysanthemum, 50-70 parts of fructus lycii, 30-50 parts of poria cocos, 30-50 parts of liquorice, 20-40 parts of honeysuckle, 10-20 parts of momordica grosvenori, 20-40 parts of radix polygonati officinalis, 20-50 parts of mulberry, 20-50 parts of longan pulp, 20-40 parts of black date, 15-30 parts of dandelion, 20-40 parts of couch grass root, 15-30 parts of pericarpium citri reticulatae and 15-30 parts of sophora flower. The momordica grosvenori, the radix polygonati officinalis, the mulberry, the dandelion, the couch grass root and other cold heat-clearing and detoxifying medicinal materials are added to the traditional tortoise jelly formula, and the longan pulp and the black date with mild nature are matched so as to neutralize the cold and cool of the tortoise jelly, and the mauremys mutica shell of more than eight years old is selected, and the component proportion of the turtle shell is increased so as to prepare the tortoise jelly by using the ancient method; and the prepared tortoise jelly has the effects of clearing heat, reducing internal heat, enriching yin, nourishing kidney, eliminating pimples, relaxing bowel, maintaining beauty and keeping young, also has unique sweet aftertaste taste, does not have smelly medicinal materials, and is more popular with consumers.