42 results about "Mauremys mutica" patented technology

The invention discloses health mauremys mutica feed. The health mauremys mutica feed is prepared from 15-25% by weight of imported white fish meal, 10-15% by weight of imported red fish meal, 5-10% by weight of euphausiid powder, 5-10% by weight of imported meat and bone meal, 4-10% by weight of fermented soybean meal, 3-5% by weight of soybean meal, 2-5% by weight of pumpkin powder, 3-5% by weight of wall-broken beer yeast, 16-23% by weight of wheat powder, 1-3% by weight of mixed oil, 1-3% by weight of spiral seaweed powder, 0.3-0.5% by weight of pearl powder, 0.1-0.3% by weight of composite probiotics, 1-3% by weight of a traditional Chinese medicine regulator, 1-2% by weight of a vitamin premix, 0.5-1% by weight of a mineral matter premix, 1-2% by weight of calcium dihydrogen phosphate and 0.2-0.3% by weight of choline chloride. The health mauremys mutica feed has comprehensive nutrition, can be easily digested and absorbed, promotes mauremys mutica growth and improves a bodily form, a body color and immunity.

The invention discloses a microsatellite molecular marker of mauremys mutica. The nucleotide sequence of the microsatellite molecular marker is SEQ ID NO:1. The invention also discloses a primer of the microsatellite molecular marker of the mauremys mutica. The microsatellite molecular marker of the mauremys mutica can be used for carrying out genetic structure analysis, genetic map construction, gene location, auxiliary marking seed selection or auxiliary marking seed breeding on the mauremys mutica.

The invention discloses an artificial indoor feeding method for Asian yellow pond turtles in the south. A natural temperature and artificially controlled humidity incubation technology is adopted, and the incubation time is shortened; spatial frame erection and layered pond arrangement for three-dimensional aquaculture are utilized, the method is simple and feasible, and various spaces are sufficiently utilized; a hibernation-free aquaculture technology is adopted, the Asian yellow pond turtles do not have the growing hibernation stage, grow fast, are good in quality and high in benefit and are suitable for being cultured in rooms of different families, and a foundation is laid for industrialization; an aquaculture income-increasing method is provided for people in all ages, particularly office workers in urban and rural families with water and electricity.

The invention discloses a simple method for hatching the male larva of mauremys mutica, belonging to the technical field of control on the male gender of terrapin animals. The method comprises the following steps: 1) during the mating season of the mauremys mutica, putting the long diameter of fertilized ovum into a hatching matrix, enabling embryos to be upward, and covering a hatching matrix with same humidify on the fertilized ovum; 2) horizontally arranging a hatching box on a bracket in an artificial cellar, controlling the temperature and the air humidity in the cellar, and hatching for 55-65 days in the cellar; and 3) when 23 circles of developmental duration for hatching the ovum of the mauremys mutica into the embryos in the cellar finishes, horizontally moving the hatching box into a common hatching room to continue to hatch, and obtaining the larva of the mauremys mutica by hatching for 10-20 days. According to the method, the gender of the newly born larva of the mauremys mutica can be controlled effectively, the ovum hatching rate is increased, and the larva quality is enhanced.

The invention discloses application of a mauremys mutica polypeptide mixture in the field of antitumor drug preparation and further discloses an antitumor drug which contains the mauremys mutica polypeptide mixture. The mauremys mutica polypeptide mixture has an excellent antitumor effect, and according to experimental verification, the mauremys mutica polypeptide mixture is capable of evidently inhibiting proliferation of breast cancer cells MDA-MB-231 and MCF-7, retarding a G2-M period of MDA-MB-231 and an S period of MCF-7 and evidently inhibiting proliferation of breast cancer cells MCF-7 in BALB / c-Nude mice bodies. Therefore, the mauremys mutica polypeptide mixture has a promising application prospect in the field of antitumor drug preparation.

The invention discloses a compounded edible fungus meat product and a processing method thereof. The method comprises the following steps: by taking fresh edible fungi and mauremys mutica as raw materials, firstly carrying out rinsing, color-protecting, slicing, precooking, salting, desalting and the like on the edible fungi so as to prepare a semi-product; carrying out rinsing, fishy smell removing, precooking, bone soup flavoring, cooling, slitting and the like on the mauremys mutica so as to prepare a semi-product; proportionally combining the semi-product of the edible fungi with the semi-product of the mauremys mutica, adding spices, seasonings and auxiliary materials and evenly mixing so as to obtain the compounded edible fungus meat product. The compounded edible fungus meat product has the advantages of being green and natural, reasonable in nutritional matching, excellent in flavor, good in taste, convenient to eat, long in shelf life and the like.

The invention discloses a preparation method of mauremys mutica polypeptide. The preparation method comprises the following steps: adding ultra-pure water to minced mauremys mutica meat according to a solid-to-liquid ratio of 1 to (5-10), so as to obtain minced meat liquid; adding neutral protease to the minced meat liquid, and conducting enzymatic hydrolysis for 8-12h so as to obtain enzymatic hydrolysate; conducting an enzyme deactivating operation on the enzymatic hydrolysate so as to obtain a mauremys mutica polypeptide solution; and centrifuging the mauremys mutica polypeptide solution and removing a precipitate, and freeze-drying the mauremys mutica polypeptide solution so as to obtain mauremys mutica polypeptide. The extracted mauremys mutica polypeptide provided by the invention, which is 2000-3000 in mean molecular mass range, belongs to small-molecular bioactive polypeptide, and the mauremys mutica polypeptide has an anti-oxidation effect and is capable of resisting oxygen free radicals. The invention also discloses a mauremys mutica polypeptide facial mask.

The invention discloses a hatching method of Mauremys mutica. In propagation season, ventilate plastic box is taken as hatching box. Vermiculite layer of 3-6cm thickness is laid on the bottom of the hatching box as hatching medium. Fertilized eggs are put on the hatching medium with the space of 0.3-0.8cm, and then another layer of vermiculite of 2-3cm thickness is covered on the fertilized eggs. The humidity of the vermiculite layers in the whole hatching period is maintained 15 percent-20 percent, and the temperature in the whole hatching period is controlled 25-33 centigrade. Young tortoises are hatched 55-63 days. The invention adopts the vermiculite as the hatching medium, makes use of the preferable warmth retention property, moisture retention property and permeability of the vermiculite, improves the hatching success rate of the Mauremys mutica, shortens the hatching period and enhances the quality of newborn young tortoises.

The invention discloses application of mauremys mutica polypeptide mixtures to the field of preparation of fatty liver prevention and treatment healthcare products or fatty liver prevention and treatment medicines, a fatty liver prevention and treatment healthcare product or a fatty liver prevention and treatment medicine with the mauremys mutica polypeptide mixtures. The application, the fatty liver prevention and treatment healthcare product or the fatty liver prevention and treatment medicine have the advantages that the mauremys mutica polypeptide mixtures have excellent liver healthcare functions; as verified by fatty liver model tests implemented by HepG2 cells induced by different inducers, the contents of TG (triglyceride) and LDL-C (low-density lipoprotein-cholesterol) in the HepG2 fatty liver cells induced by the different inducers including oleic acid, glucose and alcohol can be effectively reduced by the mauremys mutica polypeptide mixtures, the content of lipid in fatty livers of the HepG2 cells induced by the different inducers including the oleic acid, the glucose and the alcohol can be obviously reduced by the mauremys mutica polypeptide mixtures and oil red O staining mauremys mutica polypeptide mixtures which are combined with one another, and accordingly the mauremys mutica polypeptide mixtures have excellent application prospects in the field of preparation of the fatty liver prevention and treatment healthcare products or the fatty liver prevention and treatment medicines.

The invention discloses a preparation method for a mauremys mutica feed, which comprises the following components: 9-11wt% of fresh chicken liver, 7-11wt% of duck entrails, 5-7wt% of sheep entrails, 6-9wt% of fresh trash fish, 11-15wt% of fresh freshwater shrimp, 2-4wt% of fresh whitefish blocks, 6-8wt% of fresh shrimp, 6-8wt% of fresh yellow mealworm, 2-4wt% of fresh earthworm, 8-11wt% of fresh soybean granules, 4-6wt% of fresh carrot blocks, 2-4wt% of fresh loofah blocks, 6-8wt% of bone powder, 1-3wt% of vitamins, 1-3wt% of salt, 0.5-1.5wt% of pipewort powder, 0.5wt%-1.5wt% of meadowrue root and rhizome powder, 0.5-1.5wt% of honeysuckle powder, 0.5-1.5wt% of sargentg loryvine powder, 5-1.5wt% of rabdosia rubescens powder, and 5-1.5wt% of starwort root powder.

The invention discloses a method of hatching Yellow throat quasi-water tortoise, in the breeding season, employing ventilating plastic box as hatching box, laying a 3-6cm -thick sand grain layer on the box bottom as hatching base material, putting the fertilized ovum into the box, 2-3 fertilized ovum for every 100cm2, covering a 1-3cm-thick sand grain layer, the water content of the hatching bed during the whole hatching period is maintained 5-10%; and the temperature is controlled in 28-30 Deg C, then getting the infant tortoise with balanced sex ratio after 65-70 days later. The invention can increase the success rate of hatching Yellow throat quasi-water tortoise, and the survival rate of the infant tortoise.

The invention relates to the field of aquaculture, and particularly relates to compound feed of Mauremys mutica. The compound feed comprises the following components in parts by weight: 15-20 parts of imported steamed fish meal, 18-25 parts of domestic fish meal, 10-15 parts of domestic meat and bone meal, 2-5 parts of spray-dried blood cells, 25-40 parts of de-hulled soybean meal, 17-23 parts of special second-class flour, 1.0-2.0 parts of calcium biphosphate, 0.13-0.30 part of choline chloride, 0.03-0.05 part of an antioxidant and 1.0-3.0 parts of soybean oil as well as multiple vitamin additives and multiple mineral additives.

The invention discloses a breeding method for yellow pond turtles, and relates to the technical field of turtle breeding. The breeding method is characterized by including the following steps: in the young turtle period, before the young turtles enter a pool, immersion disinfection through 1.5 mg / L of high manganese potassium is needed, then the young turtles are placed into the young turtle pool with the water temperature ranging from 25 DEG C to 30 DEG C to be fed, are fed with a mixture of cooked silkworm chrysalis, millets, eggs and the like after 3 days, and can be transferred into an adult turtle pool to be fed after 90 days; in the adult turtle period, the water temperature is kept to range from 23 DEG C to 30 DEG C, hibernation of the adult turtles can be helped, the adult turtles can hibernate by themselves when the temperature drops to 15 DEG C at the end of October each year, a person needs to pay attention to warm water bathing before hibernation, feces is thoroughly removed, a sorting box with humid soil is prepared, the adult turtles can dig holes by themselves for hibernation, the humidity needs to the controlled in the hibernation period, and turtle dehydration is prevented. The breeding method enables feeding to be reasonable, the temperature control temperature difference is smaller, feeding in the state similar to the temperature constant state is achieved, growth of the yellow pond turtles is facilitated, and the growth speed of the young turtles is high.

A functional aquaculture net cage for yellow pond turtle comprises a cage top, a cage side and a cage bottom and is characterized in that the cage top is a circular net sewn with bite-proof small-meshed superhigh molecular weight polyethylene nets, the cage side which is circular is formed by sequentially connecting copper alloy woven meshes in a semi-flexible manner, foam floats disposed on middle ropes of the copper alloy woven meshes forming a ring form a floating system, the cage bottom in a two-layer structure is formed by binding a plastic board and a copper alloy woven mesh, upper ropes of the cage side nets are sewn to an outer circular rope of the cage top net, lower ropes of the cage side nets are sewn to the edge of the cage bottom, four sinkers are hung at four corners of a cage body respectively, a floating rest plate is disposed in the cage, the cross section of the floating rest plate is in the shape of an equilateral trapezium, an upper line of the equilateral trapezium is 30cm away from a lower line, submerged part of the floating rest plate is 5cm-8cm in height, and the middle ropes of the cage are connected with a floating rope frame of a cage mooring system in an aquaculture water through mooring ropes.

The invention discloses a compound preparation for enhancing immunity of postoperative tumor patients. The compound preparation mainly comprises the following component raw materials in parts by weight: 100-400 parts of a mauremys mutica cantor protein peptide extract, 50-400 parts of a Chinese wolfberry fruit polysaccharide extract and 50-400 parts of an anoectochilus roxburghii aqueous extract. The compound preparation disclosed by the invention is used for enabling the postoperative tumor patients to recover and improving the organism immunity of the postoperative tumor patients.

The invention discloses a breeding method for yellow pond turtles, and relates to the technical field of turtle culture. The breeding method is characterized by comprising the following steps: selecting water quality: pool water is green brown, the pH value is 7.2-8.5, and the transparency is 35-50cm; selecting baits: the yellow pond turtles are omnivorous animals, and fishes, shrimps, earthworms, flour weevils, spiral shells, clams and animal giblets are used as animal fodder; feeding: in April-May and September-October, feeding once at about nine o'clock in the morning every day, and in June-August, respectively feeding once at about seven o'clock in the morning and at five o'clock in the afternoon, wherein the baits are thrown to bait platforms or a feeding yard, and the feeding amount accounts for about 5 percent of the total body weight of the turtles; performing daily management: patrolling a turtle pool for at least two times every day so as to mainly observe the feeding and movement conditions of the turtles and check whether the turtles escape and injured or not; cleaning the bait platforms every day, and planting shade plants at pool sides and building a shade shed at the upper part of the pool in summer. The breeding method has the advantages that treatment is convenient, the death rate is reduced, feeding is fixed in amount, position and time, and large-scale breeding is facilitated.

The invention discloses a maca brown rice capable of improving body fatigue resistance, which comprises the following components in parts by weight: shelled brown rice, soybean protein powder, giant rice germ, buckwheat dietary fiber, maca, black fruit wolfberry, sunflower pollen , Shigui active peptide essence, taurine, creatine, vitamins, fungal amylase, glucoamylase, etc. The invention prepares maca brown rice by using broken maca, black fruit wolfberry, sunflower pollen, shelled brown rice, soybean protein powder, buckwheat powder and nutrient substance active peptide to prepare maca brown rice, and the product has good quality, good taste and flavor , Maca, black fruit wolfberry, sunflower pollen and active peptides contained in tortoise can resist fatigue, quickly replenish physical strength, relieve stress, improve sleep quality, comprehensively adjust nutritional balance, improve immunity, strengthen the body, and stay away from sub-health, black fruit wolfberry Rich in black fruit pigment natural proanthocyanidins, it can effectively reduce fatigue by reducing the level of free radicals and oxidative stress in the body under fatigue.

The invention relates to a feed capable of increasing the laying rate of mauremys mutica. The feed is prepared from the following raw materials in parts by weight: 24 to 41 parts of chicken testis powder, 17 to 20 parts of Chinese yam powder, 20 to 24 parts of corn flour, 7 to 9 parts of vitamin powder, 8 to 11 parts of cod-liver oil, 1 to 2 parts of pig bag powder, 0.8 to 1.2 parts of mussel powder, 1.0 to 1.8 parts of amino acid, 1 to 2 parts of calcium dihydrogen phosphate, 1.2 to 2.0 parts of mineral matter, and 2 to 3 parts of traditional Chinese medicine powder, wherein the traditional Chinese medicine powder is composed of the following components in parts by weight: 0.5 to 0.7 part of bighead atractylodes rhizome, 0.2 to 0.4 part of medicated leaven, 0.3 to 0.5 part of malt, 0.4 to 0.6 part of rice sprout, 0.3 to 0.4 part of rhodomyrtus tomentosa powder, and 0.3 to 0.4 part of stephania sinica. The feed is developed on the basis of nutrient needs of mauremys mutica, is rich in nutrients, does not contain any hormone or chemical residue, and is capable of increasing the laying rate of mauremys mutica.

The invention belongs to the technical field of nutrition, and in particular relates to a health care product for constitution with Yin-deficiency and a preparation method of the health care product. The health care product consists of the following materials in parts by dry weight: 15-25 parts of herba dendrobii, 5-15 parts of American ginseng, 5-15 parts of radix glehniae, 5-25 parts of mauremys mutica shell and 10-20 parts of turtle shell. According to the health care product disclosed by the invention, the adopted materials are derived from special regions, the materials not only are safe and free from toxic effects but also contain chemical components at special proportions, the materials are mixed according to a certain proportion and are matched in accordance with the function and efficacy of each material so as to cause a synergistic effect; and the health care product has a significant curative effect on the Yin-deficiency constitution with the symptoms of soreness and weakness of waist and knees, dizziness and tinnitus, skinny body form, night sweat, dry lips and dry throat, short and yellow urine, insomnia and dreaminess, emaciation and poor appetite, eye dryness, constipation, irregular menstruation or hypomenorrhea and even amenorrhoea, premature ejaculation, impotence, three high (high blood fat, high blood pressure and high blood sugar), being upset, fatigue and weakness, acne in face, lusterless complexion and the like.

The invention provides a method for incubating mauremys mutica eggs and relates to the technical field of turtle cultivation. The method is characterized by comprising the following steps that a wooden box specially manufactured is adopted as an incubator, the size is 60*30*10 cm, water holes are drilled in the bottom of the incubator or around the incubator, a layer of clean sandy soil is placed on the bottom of the incubator, the thickness is 5 cm, the turtle eggs are placed on the soil, the distance between every two turtle eggs is 1 cm, then the eggs are covered with a layer of sandy soil with the thickness of 2 cm, and then covered with a piece of clean wet cloth, after being placed well, the turtle eggs can not be moved at will, the incubating temperature is controlled to range from 28 DEG C to 35 DEG C, the water content of the sandy soil is 5-10%, the sandy soil is held in hands to form balls, and gets loose after being released, small turtles are incubated out after 45-55 days, 5% of saline solution is used for disinfecting the small turtles coming out of shells, and the small turtles can be fed on food such as boiled egg yolk, daphnia, shrimps and tubifex hattai. The setting conditions are processed reasonably, the wooden box is utilized as the incubator, large-scale processing is carried out, and the incubating rate can be increased easily.

The invention belongs to the technical field of aquaculture, and particularly discloses a hybridization breeding method of yellow sand turtle and Modi turtle. The hybrid breeding method of the yellowsand turtle and the Modi turtle comprises the following steps of: (1) using a pure yellow sand turtle with sexual maturity as a male parent and a pure Modi turtle with sexual maturity as a female parent to mate so as to obtain a first filial generation; (2) using the first filial generation with sexual maturity as a male parent with a pure Modi turtle with sexual maturity as a female parent to mate so as to obtain a second filial generation; (3) using the first filial generation with sexual maturity as a male parent with the second filial generation with sexual maturity as a female parent mateso as to obtain a third filial generation; and (4) using the third filial generation with sexual maturity as a male parent and a female parent to mate so as to obtain a hybrid turtle species. The yellow sand turtle and the Modi turtle are hybridized according to a specific method, and the bred hybrid turtle species has the advantages of high meat content rate, strong reproductive capacity and disease resistance, and greatly improves the commodity quality and breeding benefit of turtle breeding.

The invention discloses a formula of a feed for mauremys mutica and a preparation method thereof. The feed is prepared from following raw materials, by weight, 40-50 parts of pig viscera, 25-30 parts of chicken viscera, 25-28 parts of viscera of big fish, 35-40 parts of small fish, 20-24 parts of marine shrimp, 16-18 parts of earthworms, 10-14 parts of snails, 12-16 parts of spiral shells, 8-12 parts of shells, 15-20 parts of chicken bone, 15-18 parts of fish bone, 12-16 parts of yeast powder, 20-24 parts of corn flour, 2-4 parts of composite vitamin, 5-7 parts of mineral salt, 20-24 parts of aquatic plants, 16-18 parts of vegetables, 1-2 parts of honeysuckle powder and 1-2 parts of bupleurum falcatum powder. The feed contains abundant proteins and vitamins and is reasonable in nutrient combination. The feed contains the honeysuckle powder and the bupleurum falcatum powder so that the feed reduces disease rate of the mauremys mutica and reinforces body quality, increases growth speed and anti-disease capability of the mauremys mutica. The feed is low in cost and employs easy-to-obtain materials, is simple in formula and processing method, is low in pollution rate on water and is reduced in pollution on water body.

The invention provides a method for judging mauremys mutica embryonic development phase without dissection, and belongs to the field of temperature-dependent sex determination animal sex differentiation and sex regulation and control. In a mauremys mutica incubation season, mauremys mutica zygotes are collected for incubation; the change condition of fertilization spots is observed in the whole incubation process; the change condition of blood streaks is observed through light illumination and photos are taken for recording; in a first week of the incubation, the phase change is fast, and embryos are too small to be accurately judged by naked eye observation; embryo dissection observation is not performed in the first week of the incubation; 4 to 6 zygotes are taken every day for appearance observation from the eighth day of the incubation; then, the dissection is performed; the embryo development condition is observed in a stereoscopic microscope; text description and photo taking recording are performed; the embryonic development phase of Chinese softshell turtles is divided according to the division standard of the mauremys mutica embryonic development phase. The zygote dissection is not needed; through the appearance observation on the zygotes, the accurate development phase of the whole process before the starting of a mauremys mutica temperature sensitive period to the embryonic development completion can be obtained.

The invention discloses an asian yellow pond turtle feed and relates to the technical field of turtle culture. The asian yellow pond turtle feed is characterized by comprising the following components in percentage by weight: 40-45% of aquatic insects, 25-30% of fishes, 8-12% of tadpoles, 5-7% of yellow mealworm, 3-5% of water algae, 2-4% of earthworms, 1-3% of shrimp and 2-5% of fruits. A preparation method of the asian yellow pond turtle feed comprises the following steps: removing hard shells and sharp burs of the aquatic insects, the fishes and the shrimps, then mixing the aquatic insects, the fishes and the shrimps with the tadpoles, the yellow mealworm, the water algae, the earthworms, the fruits and the vitamin C, crushing and stirring the mixture to prepare insect powder. The asian yellow pond turtle feed is reasonable in nutrition cooperation and comprehensive in nutrition; the foods are similar to the food types and component ratios in the wild environment; the growth of the asian yellow pond turtle can be accelerated; the immunity of the asian yellow pond turtle can be improved.

The invention discloses a mauremys mutica egg hatching method. According to the method, no medium such as sand or vermiculite is needed, and mauremys mutica eggs are hatched directly. The biggest advantage of the method is that the method is simple. With the method, observation is convenient; hatching manpower can be reduced; hatching cost can be reduced; air can be ventilated; and pathogen infection changes are reduced. The hatching temperature is higher by 2-3 DEG C than that of a method adopting a medium. The hatching time is approximately 60 days. A hatching rate reaches 98%.

The invention provides a special feed for mauremys mutica. The special feed consists of the following raw materials in parts by weight of 20-30 parts of corn flour, 20-30 parts of soybean meal, 20-40parts of wheat, 10-16 parts of shrimp meat, 20-26 parts of fresh fish meat, 12-16 parts of pumpkins, 8-12 parts of carrot meal, 10-16 parts of pork livers, 30-40 parts of oat, 20-30 parts of sweet potatoes, 1-5 parts of fish oil, 8-12 parts of protein powder, 8-16 parts of locust powder, 16-20 parts of spirulina and 10-20 parts of rapeseed meal. The special feed is reasonable in formula, rich in nutrients, and comprehensive in components, meets growth requirements of the mauremys mutica, promotes healthy growth of the mauremys mutica, and also has the effects of strengthening constitutions andimproving immunity.

The invention discloses a Chinese herbal medicinal composition for feeding Asian yellow pond turtles, and belongs to the technical field of breeding of Asian yellow pond turtles with Chinese medicinal herbs. The Chinese herbal medicinal composition is prepared from the following raw materials: Chinese herbal medicinal powder, corn flour, glucose, shrimp meal, calcium powder, minced fish meat and bioactive polypeptides. The invention further discloses preparation and use methods of the Chinese herbal medicinal composition. The Chinese herbal medicinal powder used in the Chinese herbal medicinal composition is combined from Chinese medicinal herbs according to the principle of monarch, minister, assistant and guide, so that the healthy growth and development of the Asian yellow pond turtles is promoted, the Asian yellow pond turtles are uniform in shapes and strong in muscles and bones, diseases of the Asian yellow pond turtles can be prevented at the same time, and the disease prevention cost is lowered. Moreover, the polypeptides are supplemented reasonably in the Chinese herbal medicinal composition, so that the tortoise plastrons of the Asian yellow pond turtles become thicker and harder. In the preparation method disclosed by the invention, plant cells are subjected to full wall breaking through an ultrafine smashing technology, so that the Asian yellow pond turtles can absorb nutritional ingredients of the Chinese medicinal herbs better. The Chinese herbal medicinal composition disclosed by the invention can be applied to breeding of small water turtles and Annam turtles.

The invention relates to an indoor culture pond for Mauremys mutica. The culture pond is divided into three ponds, wherein intercommunicated space is formed among the three ponds; and water injection, water discharge, cleaning and disinfection can be conducted separately in each pond. A gentle treatment method is used for sterilization and disinfection of a Mauremys mutica culture environment and the Mauremys mutica; diseases are prevented; and stress responses are avoided.